Kyushu University Academic Staff Educational and Research Activities Database
List of Papers
Hidenori Tachida Last modified date:2018.06.07

Professor / dynamic biology / Department of Biology / Faculty of Sciences

1. Etsuko Moritsuka, Phourin Chhang, Shuichiro Tagane, Hironori Toyama, Heng Sokh, Tetsukazu Yahara, Hidenori Tachida, Genetic variation and population structure of a threatened timber tree Dalbergia cochinchinensis in Cambodia, Tree Genetics and Genomes,, 13, 6, 2017.12, Dalbergia cochinchinensis Pierre ex Laness. (Fabaceae) is a commercially important tree in Southeast Asia. Although this species is under legal protections, illegal logging and disorderly developments have reduced its populations, and the conservation of this species is currently of much concern. In this study, we determined nucleotide sequences at six chloroplasts and ten nuclear loci in four populations of D. cochinchinensis in Cambodia, followed by population genetic analyses. The average silent nucleotide diversity over the nuclear loci, excluding one with an exceptionally high value, was 0.0057 in the entire population, and the mean FST across the nuclear loci between each population pair was between 0.135 and 0.467. Thus, the nucleotide diversity in the studied populations was not low compared with that in other tree species, and the level of population differentiation was high. Neutrality test statistics indicated a recent reduction of population size and a subdivision of the population within this species. The divergence times and migration rates were estimated with a likelihood-based method assuming the isolation with migration model. Based on the results, the three populations split 68,000–138,000 years ago, possibly corresponding to the start of the last glacial period, and the level of gene flow among the populations was very low thereafter. Moreover, after the split, population sizes were reduced considerably. Notably, the nucleotide diversity in an insertion sequence in a noncoding region of nuclear C4H was much higher than the mean nucleotide diversity in silent sites across other nuclear genes, indicating that the region was affected by selection..
2. Makiko Mimura, Tetsukazu Yahara, Daniel P. Faith, Ella V�zquez-Dom�nguez, Robert I. Colautti, Hitoshi Araki, Firouzeh Javadi, Juan N��ez-Farf�n, Akira S. Mori, Shiliang Zhou, Peter M. Hollingsworth, Linda E. Neaves, Yuya Fukano, Gideon F. Smith, Yo Ichiro Sato, Hidenori Tachida, Andrew P. Hendry, Understanding and monitoring the consequences of human impacts on intraspecific variation, Evolutionary Applications,, 10, 2, 121-139, 2017.02, Intraspecific variation is a major component of biodiversity, yet it has received relatively little attention from governmental and nongovernmental organizations, especially with regard to conservation plans and the management of wild species. This omission is ill-advised because phenotypic and genetic variations within and among populations can have dramatic effects on ecological and evolutionary processes, including responses to environmental change, the maintenance of species diversity, and ecological stability and resilience. At the same time, environmental changes associated with many human activities, such as land use and climate change, have dramatic and often negative impacts on intraspecific variation. We argue for the need for local, regional, and global programs to monitor intraspecific genetic variation. We suggest that such monitoring should include two main strategies: (i) intensive monitoring of multiple types of genetic variation in selected species and (ii) broad-brush modeling for representative species for predicting changes in variation as a function of changes in population size and range extent. Overall, we call for collaborative efforts to initiate the urgently needed monitoring of intraspecific variation..
3. Yuka Ikezaki, Yoshihisa Suyama, Beth A. Middleton, Yoshihiko Tsumura, Kosuke Teshima, Hidenori Tachida, Junko Kusumi, Inferences of population structure and demographic history for Taxodium Distichum, a coniferous tree in North America, based on Amplicon sequencing analysis, American Journal of Botany,, 103, 11, 1937-1949, 2016.11, PREMISE OF THE STUDY: Studies of natural genetic variation can elucidate the genetic basis of phenotypic variation and the past population structure of species. Our study species, Taxodium distichum, is a unique conifer that inhabits the flood plains and swamps of North America. Morphological and ecological differences in two varieties, T. distichum var. distichum (bald cypress) and T. distichum var. imbricarium (pond cypress), are well known, but little is known about the level of genetic differentiation between the varieties and the demographic history of local populations. METHODS: We analyzed nucleotide polymorphisms at 47 nuclear loci from 96 individuals collected from the Mississippi River Alluvial Valley (MRAV), and Gulf Coastal populations in Texas, Louisiana, and Florida using high-throughput DNA sequencing. Standard population genetic statistics were calculated, and demographic parameters were estimated using a composite-likelihood approach. KEY RESULTS: Taxodium distichum in North America can be divided into at least three genetic groups, bald cypress in the MRAV and Texas, bald cypress in Florida, and pond cypress in Florida. The levels of genetic differentiation among the groups were low but significant. Several loci showed the signatures of positive selection, which might be responsible for local adaptation or varietal differentiation. CONCLUSIONS: Bald cypress was genetically differentiated into two geographical groups, and the boundary was located between the MRAV and Florida. This differentiation could be explained by population expansion from east to west. Despite the overlap of the two varieties’ ranges, they were genetically differentiated in Florida. The estimated demographic parameters suggested that pond cypress split from bald cypress during the late Miocene..
4. Junko Kusumi, Yoshihiko Tsumura, Hidenori Tachida, Evolutionary rate variation in two conifer species, taxodium distichum (L.) rich. var. distichum(baldcypress) and cryptomeria Japonica(Thunb. ex L.F.) D. Don (Sugi, Japanese Cedar), Genes and Genetic Systems,, 90, 5, 305-315, 2016.03, With the advance of sequencing technologies, large-scale data of expressed sequence tags and full-length cDNA sequences have been reported for several conifer species. Comparative analyses of evolutionary rates among diverse taxa provide insights into taxon-specific molecular evolutionary features and into the origin of variation in evolutionary rates within genomes and between species.Here, we estimated evolutionary rates in two conifer species, Taxodium distichumand Cryptomeria japonica, to illuminate the molecular evolutionary features of these species, using hundreds of genes and employing Chamaecyparis obtusa as an outgroup. Our results show that the mutation rates based on synonymous substitution rates (dS) of T. distichum and C. japonica are approximately 0.67 × 10–9and 0.59 × 10–9/site/year, respectively, which are 15–25 times lower than those of annual angiosperms. We found a significant positive correlation between dS and GC3. This implies that a local mutation bias, such as context dependency of the mutation bias, exists within the genomes of T. distichum and C. japonica, and/or that selection acts on synonymous sites in these species. In addition, the means of the ratios of synonymous to nonsynonymous substitution rate in the two species are almost the same, suggesting that the average intensity of functional constraint is constant between the lineages. Finally, we tested the possibility of positive selection based on the site model, and detected one candidate gene for positive selection..
5. Miho Tamura, Yosuke Hisataka, Etsuko Moritsuka, Atsushi Watanabe, Kentaro Uchiyama, Norihiro Futamura, Kenji Shinohara, Yoshihiko Tsumura, Hidenori Tachida, Analyses of random BAC clone sequences of Japanese cedar, Cryptomeria japonica, Tree Genetics and Genomes,, 11, 3, 2015.06, Conifers have larger genomes than most angiosperms, long generation times, and undergone relatively few chromosome duplications during their evolution. Thus, conifers are interesting targets for molecular evolutionary studies. Despite this, there have been few studies regarding their genome structure, and these studies are mostly limited to the Pinaceae. Our target species, Cryptomeria japonica, belongs to the Cupressaceae family, which is phylogenetically separated from the Pinaceae family by a few hundred million years, and is the most important timber tree in Japan, making investigation of its genome structure both interesting and worthwhile. We analyzed the sequences of eight random bacterial artificial chromosome (BAC) clones from C. japonica and compared them with sequences of comparable size from eight other model plants, including Arabidopsis thaliana and Pinus taeda. From this analysis, we identified several features of the C. japonica genome. First, the genome of C. japonica has many divergent repetitive sequences, similar to those of Physcomitrella patens and P. taeda. Additionally, some C. japonica transposable elements (TEs) seem to have been active until recently, and some might be unidentified novel TEs. We also found a putative protein-coding gene with a very long intron (approximately 70 kb). The three Pinaceae species whose genome sequences have been determined share these features, despite the few hundred million years of independent evolution separating the Pinaceae species from C. japonica..
6. Tomotaka Matsumoto, Akiko A. Yasumoto, Kozue Nitta, Shun K. Hirota, Tetsukazu Yahara, Hidenori Tachida, Difference in flowering time can initiate speciation of nocturnally flowering species, Journal of Theoretical Biology,, 370, 61-71, 2015.04, Isolation mechanisms that prevent gene flow between populations prezygotically play important roles in achieving speciation. In flowering plants, the nighttime flowering system provides a mechanism for isolation from diurnally flowering species. Although this system has long been of interest in evolutionary biology, the evolutionary process leading to this system has yet to be elucidated because of the lack of good model species. However, the genetic mechanisms underlying the differences in flowering times and the traits that attract pollinators between a pair of diurnally and nocturnally flowering species have recently been identified in a few cases. This identification enables us to build a realistic model for theoretically studying the evolution of a nocturnally flowering species. In this study, based on previous experimental data, we assumed a model in which two loci control the flowering time and one locus determines a trait that attracts pollinators. Using this model, we evaluated the possibility of the evolution of a nocturnally flowering species from a diurnally flowering ancestor through simulations. We found that a newly emerging nighttime flowering flower exhibited a sufficiently high fitness, and the evolution of a nocturnally flowering species from a diurnally flowering species could be achieved when hybrid viability was intermediate to low, even in a completely sympatric situation. Our results suggest that the difference in flowering time can act as a magic trait that induces both natural selection and assortative mating and would play an important role in speciation between diurnally and nocturnally flowering species pairs..
7. Tomotaka Matsumoto, Yohey Terai, Norihiro Okada, Hidenori Tachida, Sensory drive speciation and patterns of variation at selectively neutral genes, Evolutionary Ecology, 10.1007/s10682-014-9697-8, 28, 591-609, 2014.04.
8. T. Yoshida, M. Tamekuni, Tetsukazu Yahara, N. Inomata, Hidenori Tachida, Demographic history of a common pioneer tree, zanthoxylum ailanthoides, reconstructed using isolation-with-migration model, Tree Genetics and Genomes,, 10, 5, 1213-1223, 2014.01, The Japanese prickly ash, Zanthoxylum ailanthoides Siebold & Zucc. (Rutaceae), is one of the most common pioneer tree species that grow in disturbed areas, such as canopy gaps, in Japanese warm-temperate evergreen oak forests. The strong genetic structure of its current population might suggest long-term isolation of subpopulations by demographic events, in addition to ecological features of this species. Analyses of genome-wide nucleotide variation using model-based approaches are necessary to achieve a good understanding of the demographic history of a species. In this study, we analyzed the nucleotide variation in natural populations of Z. ailanthoides using a computer program that applied the isolation-with-migration model to quantitatively infer the demographic history. Nucleotide variations at 10 or 26 nuclear loci in six populations from a wide range of the species distribution were analyzed. The maximum likelihood estimate of the divergence time between the current populations in the two hypothetical refugia of Z. ailanthoides was approximately 24,000 years.Unexpectedly, the estimated size of the ancestral population was larger than the sizes of the two current populations. The results suggested a relatively recent divergence of these two populations and rapid formation of strong genetic structure among subpopulations. The large ancestral population may indicate a more complex demographic history during or after the last glacial period than the simple isolation-withmigration model implies..
9. Kazumasa Shirai, Nobuyuki Inomata, Shinji Mizoiri, Mitsuto Aibara, Yohey Terai, Norihiro Okada, Hidenori Tachida, High prevalence of non-synonymous substitutions in mtDNA of cichlid fishes from Lake Victoria, Gene,, 552, 2, 239-245, 2014.01, When a population size is reduced, genetic drift may fix slightly deleterious mutations, and an increase in nonsynonymous substitution is expected. It has been suggested that past aridity has seriously affected and decreased the populations of cichlid fishes in Lake Victoria, while geographical studies have shown that the water levels in Lake Tanganyika and Lake Malawi have remained fairly constant. The comparably stable environments in the latter two lakes might have kept the populations of cichlid fishes large enough to remove slightly deleterious mutations. The difference in the stability of cichlid fish population sizes between Lake Victoria and the Lakes Tanganyika and Malawi is expected to have caused differences in the nonsynonymous/synonymous ratio, ω (=. dN/. dS), of the evolutionary rate. Here, we estimated ω and compared it between the cichlids of the three lakes for 13 mitochondrial protein-coding genes using maximum likelihood methods. We found that the lineages of the cichlids in Lake Victoria had a significantly higher ω for several mitochondrial loci. Moreover, positive selection was indicated for several codons in the mtDNA of the Lake Victoria cichlid lineage. Our results indicate that both adaptive and slightly deleterious molecular evolution has taken place in the Lake Victoria cichlids' mtDNA genes, whose nonsynonymous sites are generally conserved..
10. Miyuki Takeda, Junko Kusumi, Shinji Mizoiri, Mitsuto Aibara, Semvua Isa Mzighani, Tetsu Sato, Yohey Terai, Norihiro Okada, Hidenori Tachida, Genetic Structure of Pelagic and Littoral Cichlid Fishes from Lake Victoria, PLoS One,, 8, 9, 2013.09, The approximately 700 species of cichlids found in Lake Victoria in East Africa are thought to have evolved over a short period of time, and they represent one of the largest known examples of adaptive radiation. To understand the processes that are driving this spectacular radiation, we must determine the present genetic structure of these species and elucidate how this structure relates to the ecological conditions that caused their adaptation. We analyzed the genetic structure of two pelagic and seven littoral species sampled from the southeast area of Lake Victoria using sequences from the mtDNA control region and 12 microsatellite loci as markers. Using a Bayesian model-based clustering method to analyze the microsatellite data, we separated these nine species into four groups: one group composed of pelagic species and another three groups composed mainly of rocky-shore species. Furthermore, we found significant levels of genetic variation between species within each group at both marker loci using analysis of molecular variance (AMOVA), although the nine species often shared mtDNA haplotypes. We also found significant levels of genetic variation between populations within species. These results suggest that initial groupings, some of which appear to have been related to habitat differences, as well as divergence between species within groups took place among the cichlid species of Lake Victoria..
11. Hitomi Kawaida, Kohki Ohba, Yuhki Koutake, Hiroshi Shimizu, Hidenori Tachida, Yoshitaka Kobayakawa, Symbiosis between hydra and chlorella: Molecular phylogenetic analysis and experimental study provide insight into its origin and evolution, Molecular Phylogenetics and Evolution, 66, 906-914, 2013.03.
12. Tomotaka Matsumoto, AkikoAYasumoto, 新田 梢, Tetsukazu Yahara, Hidenori Tachida, Difference in flowering time as an isolating barrier, Journal of Theoretical Biology, 317, 161-167, 2013.02.
13. Nakatada Wachi, Yoshihisa Abe, Nobuyuki Inomata, ALFRED EDWARD SZMIDT, Hidenori Tachida, Speciation history of three closely related oak gall wasps, Andricus mukaigawae, A. kashiwaphilus, and A. pseudoflos (Hymenoptera: Cynipidae) inferred from nuclear and mitochondrial DNA sequences
, Molecular Ecology, 21, 4681-4694, 2012.10.
14. Ayako Tanaka, Masato Ohtani, Yoshihiko Suyama, Yoshihiko Tsumura, Nobuyuki Inomata, Beth. A. Middleton, Hidenori Tachida, Junko Kusumi, Population genetic structure of a widespread coniferous tree Taxodium distichum [L.] Rich. (Cupressaceae) in the Mississippi River Alluvial Valley and Florida., Tree Genetics & Genomes, 10.1007/s11295-012-0501-z, 8, 1135-1147, 2012.08, Studies of the genetic variation can elucidate the structure of present and past populations, as well as the genetic basis of phenotypic variability of species. Taxodium distichum is a coniferous tree dominant in lowland river flood plains and swamps of the southeastern U. S. A., which exhibit morphological variability and adaption to stressful habitats. This study provides a survey of the Mississippi River Alluvial Valley (MAV) and Florida to elucidate their population structure and the extent of genetic differentiation between the two regions and sympatric varieties including baldcypress (var. distichum) and pondcypress (var. imbricatum). We determined the genotypes of 12 simple sequence repeat (SSR) loci totaling 444 adult individuals from 18 natural populations. Bayesian clustering analysis revealed high levels of differentiation between the MAV and the Florida regions. Within the MAV region, there was a significant correlation between genetic and geographical distances. In addition, we found that there was almost no genetic differentiation between the varieties. Most genetic variation was found within individuals (76.73 %), 1.67 % among individuals within population, 15.36 % among populations within the regions, and 9.23 % between regions within the variety. Our results suggest that i) the populations of the MAV and the Florida regions are divided into two major genetic groups, which might originate from different glacial refugia, and ii) the patterns of genetic differentiation and phenotypic differentiation were not parallel in this species..
15. Hidenori Tachida, Linkage disequilibrium in a population undergoing periodic fragmentation and admixture, Genes & Genetic System, 87, 2, 125-135, 2012.04.
16. E. Moritsuka, Y. Hisataka, M. Tamura, K. Uchiyama, A. Watanabe, Y. Tsumura, and H. Tachida, Extended linkage disequilibrium in non-coding regions in a conifer, Cryptomeria japonica., Genetics, 190, 45-48, 2012.03.
17. M. Tamura and H. Tachida, Evolution of the number of LRRs in plant disease resistance genes, Molecular Genetics and Genomics, 10.1007/s00438-011-0615-2, 285, 393–402, 2011.05.
18. J. Kusumi, L. Zidong, T. Kado, Y. Tsumura,B. A. Middleton, and H. Tachida, Multilocus patterns of nucleotide polymorphism and demographic change in Taxodium distichum (Cupressaceae) in the lower Mississippi River Alluvial Valley. , American Journal of Botany, 97, 11, 1–10, 2010.11.
19. H. Kawaida, H. Shimizu, T. Fujisawa, H. Tachida, Y. Kobayakawa, Molecular phylogenetic study in genus Hydra., Gene, 468, 30–40., 2010.11.
20. S. I. Mzighani, M. Nikaido, M. Takeda, O. Seehausen, Y. L. Budeba, B. P. Ngatunga, E. F.B. Katunzi, M. Aibara, S. Mizoiri, T. Sato, H. Tachida, N. Okada, Genetic variation and demographic history of the Haplochromis laparogramma group of Lake Victoria—An analysis based on SINEs and mitochondrial DNA., Gene, 450, 39-47, 2010.05.
21. T. Yoshida, H. Nagai, T. Yahara, and H. Tachida, Genetic structure and putative selective sweep in pioneer tree, Zanthoxylum ailanthoides., J. Plant Res., 123, 607-616, 2010.05.
22. Mikiko Soejima, Hidenori Tachida and Yoshiro Koda, Sequence analysis of human TRPV6 suggests positive selection outside Africa gene., Biochem. Genet., 47:147–153., 2009.05.
23. K. Maeda, M. Takeda, K. Kamiya, M. Aibara, S. I. Mzighani, M. Nishida, S. Mizoiri, T. Sato, Y. Terai, N. Okada, H. Tachida, Population structure of two closely related pelagic cichlids in Lake Victoria, Haplochromis pyrrhocephalus and H. laparogramma. , Gene, 441, 67-73, 2009.05.
24. H. Nagai, T. Yoshida, K. Kamiya, T. Yahara and H. Tachida, Development and characterization of microsatellite markers in Zanthoxylum ailanthoides (Rutaceae). , Mol. Ecol. Res., 9: 667–669., 2009.01.
25. Akihiro Fujimoto, Tomoyuki Kado, Hiroshi Yoshimaru, Yoshihiko Tsumura and Hidenori Tachida, Adaptive and Slightly Deleterious Evolution in a Conifer, Cryptomeria japonica. , Journal of Molecular Evolution, 67: :201-210, 2008.10.
26. Ole Seehausen, Yohey Terai, Isabel S. Magalhaes, Karen L. Carleton, Hillary D. J. Mrosso, Ryutaro Miyagi, Inke van der Sluijs, Maria V. Schneider, Martine E. Maan, Hidenori Tachida, Hiroo Imai, and Norihiro Okada, Speciation through sensory drive in cichlid fish. , Nature, 455: 620-627., 2008.10.
27. K. Kamiya, E. Moritsuka, T. Yoshida, T. Yahara, and H. Tachida, High population differentiation and unusual haplotype structure in a shade-intolerant pioneer tree species, Zanthoxylum ailanthoides (Rutaceae) revealed by analysis of DNA polymorphism at four nuclear loci., Mol. Ecol., 17: 2329-2338., 2008.05.
28. K. Maeda, H. Takeshima, S. Mizoiri, N. Okada, M. Nishida and H. Tachida, Isolation and characterization of microsatellite loci in the cichlid fish in Lake Victoria, Haplochromis chilotes., Mol. Ecol. Resources, 8, 428-430., 2008.02.
29. Yohey Terai, Ole Seehause, Takeshi Sasaki, Kazuhiko Takahashi, Shinji Mizoiri, Tohru Sugawara, Tetsu Sato1,Masakatsu Watanabe1, Nellie Konijnendijk, Hillary D. J. Mrosso, Hidenori Tachida, Hiroo Imai, Yoshinori Shichida, Norihiro Okada, Divergent Selection on Opsins Drives Incipient Speciation in Lake Victoria Cichlids., PLoS Biology, 4: e433, 2006.12.
30. Junko Kusumi, Aya Sato and Hidenori Tachida, Relaxation of functional constraint on light-independent protochlorophyllide oxidoreductase in Thuja., Mol. Biol. Evol., 23 (5) 941-948., 2006.05.
31. Yasuyuki Hashiguchi, Tomoyuki Kado, Seiro Kimura and Hidenori Tachida, Comparative phylogeography of closely related freshwater fishes, Tanakia lanceolata and T. limbata (Teleostei, Cyprinidae) in western Japan, based on mitochondrial DNA analysis., Zool. Sci., 23 (4): 309-322., 2006.04.
32. T. Kado, Y. Ushio, H.Yoshimaru, Y. Tsumura and H. Tachida, Contrasting patterns of DNA variation in natural populations of closely related conifers, Cryptomeria japonica and Taxodium distichum (Cupressaceae sensu lato)., Genes & Genetic System, 82 (2): 103-113., 2006.03.
33. Mikiko Soejima, Hidenori Tachida, Takafumi Ishida, Akinori Sano, and Yoshiro Koda, Evidence for recent positive selection at the human AIM1 locus in a European population., Mol. Biol. Evol., 23: 179-188., 2006.01.
34. K. Kitamura, H. Tachida, K. Takenaka, K. Furubayashi and S. Kawano, Demographic genetics of Siebold's beech (Fagaceae, Fagus crenata Blume) populations in the Tanzawa Mountains, central Hoshu, Japan. II. Spatial differentiation and estimation of immigration rates using a stepping-stone structure., Plant Species Biology, 20, 2, 133-144, 20: 133-144., 2005.08.
35. Koich Kamiya, Ko Harada, Hidenori Tachida, and Peter Shaw Ashton, Phylogeny of PgiC gene in Shorea and its closely related genera (DIPTEROCARPACEAE), the dominant trees in South East Asian tropical rainforests., American Journal of Botany, 10.3732/ajb.92.5.775, 92, 5, 775-788, 92(5): 775-788., 2005.05.
36. M. Soejima, H. Tachida, M. Tsuneoka, O. Takenaka, H. Kimura, and Y. Koda, Evidence for Balancing Selection from Nucleotide Sequence Analyses of Human Complement 6 (C6) Gene., Ann. Hum. Genet., 69(3): 239-252., 2005.05.
37. Junko Kusumi and Hidenori Tachida, Compositional properties of green-plant plastid genomes., J. Mol. Evol., 10.1007/s00239-004-0086-8, 60, 4, 417-425, 60 (4) : 417-425., 2005.04.
38. A. Sano and H. Tachida, Gene Genealogy and Properties of Test Statistics of Neutrality Under Population Growth., GENETICS, 10.1534/genetics.104.032797, 169, 3, 1687-1697, 169: 1687-1697., 2005.03.
39. T, Kado, A. Fujimoto, L.H. Giang, M. Tuanm P. N. Hong, K. Harada, H. Tachida, Genetic structures of natural populations of three mangrove species, Avicennia marina, Kandelia candel and Lumnitzera racemosa, in Vientnam revealed by maturase sequences of plastid., Plant Species Biology, 19, 91-99, 2004.08.
40. Y. Koda, H. Tachida, M. Soejima, O. Takenaka and H. Kimura, Population differences in DNA sequence variation and linkage disequilibrium at the PON1 gene., Ann Hum Genet., 10.1046/j.1529-8817.2003.00077.x, 68, 110-119, 68(2):110-119., 2004.03.
41. Y. Koda, T. Ishida, H. Tachida, B. Wang, H. Pang, M. Soejima, and H. Kimura, DNA sequence variation of the human ABO-Secretor locus (FUT2) in New Guinean populations: possible early human migration from Africa., Human Genetics, 10.1007/s00439-003-1013-6, 113, 6, 534-541, 113: 534-541., 2003.11.
42. T. Kado, H. Yoshimaru, Y. Tsumura and H. Tachida., DNA variation in a conifer tree, Cryptomeria japonica (Cupressaceae sensu lato)., Genetics, 164: 1547-1559., 2003.08.
43. J. Kusumi, Y. Tsumura, H. Yoshimaru and H. Tachida, Molecular evolution of nuclear genes in Cupressacea, a group of conifer trees., Mol. Biol. Evol., 19, 5, 736-747, 19(5):736-747., 2002.05.
44. M. Iizuka, H. Tachida and H. Matsuda, Neutral Model under Random Environments., Genetics, 161: 381-388., 2002.01.
45. Y. Koda, H. Tachida, M. Soejima, O. Takenaka and H. Kimura, The very old (ancient) origin of a null allele se428 of the human ABO-secretor locus (FUT2)., J. Mol. Evol., 50(3) : 243-248., 2000.03.
46. A. Koga, A. Shimada, A. Shima, M. Sakaizumi, H. Tachida and H. Hori, Evidence of recent invasion of Medaka fish genome by the Tol2 transposable element., Genetics, 155: 273-281., 2000.01.
47. J. Kusumi, Y. Tsumura, H. Yoshimaru and H. Tachida, Phylogenetic relationships in Taxodiaceae and Cupressaceae based on the matK, chlL, trnL-trnF IGS region and trnL intoron sequences., Am. J. Bot., 10.2307/2656874, 87, 10, 1480-1488, 87: 1480-1488., 2000.01.
48. H. Tachida, DNA evolution under weak selection., Gene, 10.1016/S0378-1119(00)00475-3, 261, 1, 3-9, 261: 3-9, 2000.12.
49. K. Miyake, H. Tachida, Y. Ohshima, R. Arai, S. Kimura, N. Imada and T. Honjo, Genetic variation at the cytochrome b locus in the rosi bittering, Rhodeus ocellatus (Cyprinidae) in Japan., Ichthyol. Res., 48: 105-110., 2001.01.
50. Y. Koda, H. Tachida, H. Pang, Y. Liu, M. Soejima, A. A. Ghaderi, O. Takenaka and Hi. Kimura, Contrasting Patterns of Polymorphisms at ABO-Secretor Gene (FUT2) and Plasma a(1,3)Fucosyltransferase Gene (FUT6)., Genetics, 158, 2, 747-756, 158: 747-756., 2001.01.
51. H. Tachida, Molecular evolution in multisite nearly neutral mutation model., J. Mol. Evol., 50, 1, 69-81, 50(1): 69-81., 2000.01.
52. T. Kajita, K. Kamiya, K. Nakamura, H. Tachida, R. Wcikneswari, Y. Tsumura, H. Yoshimaru and T. Yamazaki, Molecular phylogeny of Diperocarpaceae in Southeast Asia based on nucleotide sequences of matK, trnL intron and trnL-trnF intergenic spacer region in chrolplast DNA., Mol. Phyl. Evol., 10.1006/mpev.1998.0516, 10, 2, 202-209, 10(2): 202-209, 1998.10.
53. H. Tachida and T. Kuboyama, Evolution of Multigene familes by gene duplication: a haploid model., Genetics, 149, 4, 2147-2158, Genetics 149: 2147-2158, 1998.01.