Kyushu University Academic Staff Educational and Research Activities Database
Search by Keyword How ro search
Keyword
Search Criteria
 
Detailed Search

Researcher information (To researchers) Need Help? How to update
takahiro kusakabe Last modified date：2023.11.27

Professor / Agricultural Bioresource Sciences
Department of Bioresource Sciences
Faculty of Agriculture


Graduate School
Department of Bioresource Sciences
Graduate School of Bioresource and Bioenvironmental Sciences
Undergraduate School
Department of Bioresource and Bioenvironment
School of Agriculture


Homepage
https://kyushu-u.elsevierpure.com/en/persons/takahiro-kusakabe
　Reseacher Profiling Tool　Kyushu University Pure
Academic Degree
Ph.D
Country of degree conferring institution (Overseas)
No
Field of Specialization
Insect Molecular Genetics
Total Priod of education and research career in the foreign country
02years07months
Research
Research Interests
  • Analysis of DNA damage and homologous recombination repair in insect cells
    keyword : Silkworm, Homologous recombination, DSB, DNA repair
    1999.04A double-strand break (DSB) is caused by a variety of exogenous and endogenous DNA damaging agents, including ionizing radiation and free radicals. If damage is left unrepaired or is misrepaired, it may lead cells to genetic disorder or cell death. Therefore, precise repair of DSBs is essential for maintaining the viability and genomic integrity of cells. All organisms, from bacteria to insects, and mammals, evolved to have at least two pathways for repairing DSBs; homologous recombination (HR) and non-homologous end joining (NHEJ). HR ensures accurate DSB repair using genetic information from an intact chromosome as a template. At the present study, HR repair system in insect cells is analyzed by using transgenic silkworm. Silkworm, Bombyx mori, has many unique characters related to HR. For example, female silkworm lacks meiotic recombination, but not male. The DNA repair system of an organism's genome is an extraordinarily complex process whereby a multiple of synchronized proteins reproduce the genetic material accurately as well as possible after damage. Thus, the silkworm is one of the most useful and interesting model insect for studying HR systems in molecular level because the whole genome sequence of silkworm was disclosed in this year, and more than 90,000 ESTs are available from public databases. We had already developed the sensitive assay systems for HR in cultured silkworm cells. As a next study, we plan to establish the similar systems in transgenic insects, and using these systems, we would like to reveal whether DSBs were repaired by tissue- or stage-specific manner after ionizing irradiation or artifical enzymatic induction of DSBs. In these processes, we particularly focus on the roles of HR-related genes in tissue- or stage-specific HR pathways by assessing the effects of gene knockdown using RNA interference methods. I hope that the results obtained from this proposal would make a great contribution to our understanding for DNA repair system and to application for genomic drug discovery and biotechnology.
Academic Activities
Reports
 Show All Reports >>
Papers
 Show All Papers >>
1. Hiroaki Mon, Masanao Sato, Jae Man Lee, Takahiro Kusakabe, Construction of gene co-expression networks in cultured silkworm cells and identification of previously uncharacterized lepidopteran-specific genes required for chromosome dynamics., Insect biochemistry and molecular biology, 10.1016/j.ibmb.2022.103875, 151, 103875-103875, 2022.12, Advances in sequencing technology and bioinformatics have accelerated gene discovery and homology-based functional annotation in many species, and numerous targeted gene studies have greatly expanded the understanding of gene functions. Nevertheless, there are still many genes that lack homology with genes in other evolutionary lineages and are left as genes with unknown functions. We constructed a gene co-expression network from the Bombyx mori ovary-derived cell line, BmN4, and attempted to infer the biological roles of uncharacterized genes based on the correlation between the function-known and unknown genes. Within this network, we focused on the co-expression modules involved in chromosome architecture, dynamics, and integrity, and selected the uncharacterized genes for subsequent RNAi-based phenotypic screening. This approach enabled the identification of 5 genes whose knockdown led to abnormalities in chromosome dynamics and spindle morphology in mitosis. One of them was a recently characterized gene, BmCenp-T, which plays a central role in building the kinetochore protein complex on the silkworm holocentric chromosomes. In this study, we suggest a method for constructing the gene co-expression network and selecting candidate genes for small-scale RNAi screening. This approach is complementary to homology-based annotation and may be useful for the analysis of lineage-specific uncharacterized genes such as orphan genes..
2. Akihiro Morio, Jae Man Lee, Tsuguru Fujii, Hiroaki Mon, Akitsu Masuda, Kohei Kakino, Jian Xu, Yutaka Banno, Takahiro Kusakabe, The biological role of core 1β1-3galactosyltransferase (T-synthase) in mucin-type O-glycosylation in Silkworm, Bombyx mori, Insect Biochemistry and Molecular Biology, 10.1016/j.ibmb.2023.103936, 156, 103936-103936, 2023.05.
3. Akitsu Masuda, Jae Man Lee, Takeshi Miyata, Shintaro Sato, Atsushi Masuda, Masahiro Taniguchi, Ryosuke Fujita, Hiroshi Ushijima, Keisuke Morimoto, Takeru Ebihara, Masato Hino, Kohei Kakino, Hiroaki Mon, Takahiro Kusakabe, High yield production of norovirus GII.4 virus-like particles using silkworm pupae and evaluation of their protective immunogenicity., Vaccine, 10.1016/j.vaccine.2022.12.015, 41, 3, 766-777, 2023.01, Noroviruses (NoVs) are one of the major causes of acute viral gastroenteritis in humans. Virus-like particles (VLPs) without genomes that mimic the capsid structure of viruses are promising vaccine candidates for the prevention of NoVs infection. To produce large amounts of recombinant protein, including VLPs, the silkworm-expression vector system (silkworm-BEVS) is an efficient and powerful tool. In this study, we constructed a recombinant baculovirus that expresses VP1 protein, the major structural protein of NoV GII.4. Expression analysis showed that the baculovirus-infected silkworm pupae expressed NoV VP1 protein more efficiently than silkworm larval fat bodies. We obtained about 4.9 mg of purified NoV VP1 protein from only five silkworm pupae. The purified VP1 protein was confirmed by dynamic light scattering and electron microscopy to form VLPs of approximately 40 nm in diameter. Antisera from mice immunized with the antigen blocked NoV VLPs binding to histo-blood group antigens of pig gastric mucin and also blocked NoV infection in intestinal epithelial cells derived from human induced pluripotent stem (iPS) cells. Our findings demonstrated that NoV VLP eliciting protective antibodies could be obtained in milligram quantities from a few silkworm pupae using the silkworm-BEVS..
4. Akitsu Masuda, Jae Man Lee, Takeshi Miyata, Hiroaki Mon, Keita Sato, Kosuke Oyama, Yasuteru Sakurai, Jiro Yasuda, Daisuke Takahashi, Tadashi Ueda, Yuri Kato, Motohiro Nishida, Noriko Karasaki, Kohei Kakino, Takeru Ebihara, Takumi Nagasato, Masato Hino, Ayaka Nakashima, Kengo Suzuki, Yoshino Tonooka, Miyu Tanaka, Takato Moriyama, Hirokazu Nakatake, Ryosuke Fujita, Takahiro Kusakabe, Optimization of SARS-CoV-2 Spike Protein Expression in the Silkworm and Induction of Efficient Protective Immunity by Inoculation With Alum Adjuvants., Frontiers in immunology , 10.3389/fimmu.2021.803647, 12, 803647, 2021.08, カイコ-バキュロウイルス発現系を用いて新型コロナウイルスワクチン抗原の大量生産に成功し、人への投与が認可されているAlumアジュバントを用いて十分な中和抗体誘導のうがあることを動物実験等で確認した。.
5. Akitsu Masuda, Jae Man Lee, Takeshi Miyata, Takeru Ebihara, Kohei Kakino, Masato Hino, Ryosuke Fujita, Hiroaki Mon, Takahiro Kusakabe, Stable trimer formation of spike protein from porcine epidemic diarrhea virus improves the efficiency of secretory production in silkworms and induces neutralizing antibodies in mice., Veterinary research, 10.1186/s13567-021-00971-5, 52, 1, 102-102, 2021.07, Porcine epidemic diarrhea virus (PEDV) is a highly infectious pathogen of watery diarrhea that causes serious economic loss to the swine industry worldwide. Especially because of the high mortality rate in neonatal piglets, a vaccine with less production cost and high protective effect against PEDV is desired. The intrinsically assembled homotrimer of spike (S) protein on the PEDV viral membrane contributing to the host cell entry is a target of vaccine development. In this study, we designed trimerized PEDV S protein for efficient production in the silkworm-baculovirus expression vector system (silkworm-BEVS) and evaluated its immunogenicity in the mouse. The genetic fusion of the trimeric motif improved the expression of S protein in silkworm-BEVS. A small-scale screening of silkworm strains to further improve the S protein productivity finally achieved the yield of about 2 mg from the 10 mL larval serum. Mouse immunization study demonstrated that the trimerized S protein could elicit strong humoral immunity, including the S protein-specific IgG in the serum. These sera contained neutralizing antibodies that can protect Vero cells from PEDV infection. These results demonstrated that silkworm-BEVS provides a platform for the production of trimeric S proteins, which are promising subunit vaccines against coronaviruses such as PEDV..
6. takahiro kusakabe, Proteasome inhibitor MG132 impairs autophagic flux through compromising formation of autophagosomes in Bombyx cells, 479, 690-696, 2016.11.
7. takahiro kusakabe, Characterization of the roles of DNA polymerases, clamp, and clamp loaders during S-phase progression and cell cycle regulation in the silkworm, Bombyx mori, 85, 21-29, 2016.06.
8. Daisuke Morokuma, 門 宏明, YUTAKA BANNO, takahiro kusakabe, JAE MAN LEE, Differential N-Glycan Modifications of Human Alpha 1-Acid Glycoprotein (α1AGP) Produced in Different Silkworm Strains using the Baculovirus Expression System, 84, 2, 49-53, 2016.05.
9. takahiro kusakabe, Comparative proteomic analysis of hemolymph proteins from Autographa californica multiple nucleopolyhedrovirus (AcMNPV)-sensitive or -resistant silkworm strains during infections, COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS, 10.1016/j.cbd.2015.07.003, 16, 36-47, 2015.12.
10. takahiro kusakabe, Roles of silkworm endoplasmic reticulum chaperones in the secretion of recombinant proteins expressed by baculovirus system, MOLECULAR AND CELLULAR BIOCHEMISTRY, 10.1007/s11010-015-2529-5, 409, 1-2, 255-262, 2015.11.
11. Li Zhu, Tsuneyuki Tatsuke, Jian Xu, Zhiqing Li, 門 宏明, JAE MAN LEE, takahiro kusakabe, Loqs depends on R2D2 to localize in D2 body-like granules and functions in RNAi pathways in silkworm cells, 64, 78-90, 2015.09.
12. takahiro kusakabe, Mass Production of an Active Peptide-N-Glycosidase F Using Silkworm-Baculovirus Expression System, MOLECULAR BIOTECHNOLOGY, 10.1007/s12033-015-9866-1, 57, 8, 735-745, 2015.08.
13. takahiro kusakabe, Improvement of Endo-β-N-acetylglucosaminidase H production using silkworm–baculovirus protein expression system, Journal of Asia-Pacific Entomology, 18, 2, 175-180, 2015.06.
14. takahiro kusakabe, Human alpha 1-acid glycoprotein as a model protein for glycoanalysis in baculovirus expression vector system, JOURNAL OF ASIA-PACIFIC ENTOMOLOGY, 10.1016/j.aspen.2015.03.006, 18, 2, 303-309, 2015.06.
15. 日下部 宜宏, Soaking RNAi-mediated modification of Sf9 cells for baculovirus expression system by ectopic expression of Caenorhabditis elegans SID-1, 10.1007/s00253-013-4785-1, 97, 13, 5921-5931, 2013.07.
16. Lee, J., Mon, H., Banno, Y., Iiyama, K., Kusakabe, T., Bombyx mori strains useful for efficient recombinant protein production using a baculovirus vector., J. Biotech. Biomater., 2012.06.
17. Zhu, L., Tatsuke, T., Li, Z., Mon, H., Xu, Z., Lee, J., and Kusakabe T.,, Molecular cloning of BmTUDOR-SN and analysis of its role in RNAi pathway in the silkworm, Bombyx mori (Lepidoptera: Bombycidae). , Appl. Entomol. Zool., 2012.06.
18. Sugahara, R., Mon, H., Lee, J., and Kusakabe, T.,, Monoubiquitination-Dependent Chromatin Loading of FancD2 in Silkworms, a Species Lacking the FA Core Complex., Gene, 2012.05.
19. Li, Z., Cheng, D., Mon, H., Tatsuke, T., Zhu, L., Xu, J., Lee, J., Xia, Q., and Kusakabe, T., Genome-wide identification of Polycomb target genes reveals a functional association of Pho with Scm in Bombyx mori., PLoS ONE, , 7, e34330, 2012.04.
20. Nagata, Y., Sakashita, K., Imanishi, S., Lee, J., and Kuskabe T. , Expression of glycosylated mucin-like domain using baculovirus expression system in silkworm, Bombyx mori., J. Fac. Agr. Kyushu Univ., , 57, 83-86, 2012.04.
21. Fujii, M., Takahashi, M., Mon, H., Tatsuke, T., Lee, J., and Kuskabe T., Molecular cloning of the silkworm p53R2 homolog gene. , J. Fac. Agr. Kyushu Univ., , 57, 79-82, 2012.04.
22. Li, Z., Tatsuke, T., Sakashita, K., Zhu, L., Xu, J., Mon, H., Lee, J., and Kusakabe, T., Identification and characterization of Polycomb group genes in the silkworm, Bombyx mori., Mol. Biol. Report, , 39, 5575-5588, 2012.01.
23. Mitsunobu, H., Izumi, H., Mon, H., Tatsuke, T., Lee, J., Kusakabe, T., Molecular Characterization of heterochromatin proteins 1a and 1b from the Silkworm, Bombyx mori., Insect Mol. Biol. , 21, 9-20, 2012.01.
24. Mon, H., Kobayashi, I., Ohkubo, S. Tomita, S., Lee, J., Sezutsu, H., Tamura, T., Kusakabe, T., Effective RNA interference in cultured silkworm cells mediated by overexpression of Caenorhabditis elegans SID-1., RNA Biol., , 9, 40-46, 2012.01.
25. Mon, H., Lee, J., Kawaguchi, Y., Kusakabe, T., Double-strand breaks repair by gene conversion in silkworm holocentric chromosomes, Mol. Genet. Genomics, 2011.09.
26. Mon, H., Izumi, M., Mitsunobu, H., Tatsuke, T., Iiyama, K., Jikuya, H., Lee, J., Kusakabe, T., Post-translational modifications of the N-terminal tail of histone H3 in holocentric chromosomes of bombyx mori. Insect Biochem. , Mol. Genet. Genomics, 2011.09.
27. Mitsunobu, H., Izumi, H., Mon, H., Tatsuke, T., Lee, J., Kusakabe, T., Molecular Characterization of heterochromatin proteins 1a and 1b from the Silkworm, Bombyx mori. , Insect Mol. Biol. , 2011.04.
28. Mitsunobu, H., Izumi, M., Iiyama, K., Jikuya, H., Lee, J., Mon, H., Kawaguchi, Y., Kusakabe, T. , Molecular Characterization of Core Histones in the Silkworm, Bombyx mori. , J. Insect Biotech. Seric., 79, 75-83, 2010.10.
29. Hong, S.M., Yamashita, J., Mitsunobu, H., Uchino, K., Kobayashi, I., Tamura, T., Nakajima, H., Miyagawa, H., Lee, J.M., Mon, H., Miyata, T., Kawaguchi, Y., Kusakabe, T., Efficient Soluble Protein Production on Transgenic Silkworms Expressing Cytoplasmic Chaperones using Baculovirus Expression System. , Appl. Microbiol. Biotech, 2010.04.
30. Hong, S.M., Noh, S.K., Kim, K.A., Mitsunobu, H., Mon, H., Lee, J.M., Kawaguchi, Y., Kusakabe, T. , Molecular Characterization, Localization, and Distribution of Innexins in the Silkworm, Bombyx mori. , Mol. Biotechnol. , 43, 52-58., 2009.09.
31. Mon, H., Sugahara, R., Hong, S.M., Lee, J., Kamachi, Y., Kawaguchi, Y., Kusakabe, T. , Analysis of protein interactions with two-hybrid system in cultured insect cells. , Anal. Biochem. , 392, 180-182., 2009.09.
32. Karasaki, N., Mon, H., Takahashi, M., Lee, J., Koga, K., Kawaguchi, Y., Kusakabe, T. , Establishment of Tetracycline-inducible Gene Expression Systems in the Silkworm, Bombyx mori. , Biotechnol. Lett. , 31, 495-500., 2009.06.
33. Sakashita, K., Tatsuke, T., Lee, J., Kawaguchi, Y., and Kusakabe, T. , Sequence-Nonspecific Suppression of Gene Expression by Double-stranded RNA in Silkworm Cultured Cells. , J. Insect Biotech. Seric. , 78, 33-37., 2009.06.
34. Sakashita, K., Tatsuke, T., Masaki, Y., Lee, J., Kawaguchi, Y., Kusakabe, T. , dsRNA Binding Activity of Silkworm Larval Hemolymph is Mediated by Lipophorin Complex , J. Fac. Agr., Kyushu Univ. , 54, 401-406, 2009.04.
35. Tatsuke, T., Hong, S.M., Tobata, H., Mon, H., Lee, J., Kawaguchi, Y., Kusakabe, T., Construction of piggyBac-based Vectors Using Visible and Drug-resistance Marker for Introducing Foreign Genes into Silkworm Cultured Cells. , J. Fac. Agr., Kyushu Univ. , 54, 397-400, 2009.04.
36. Lee, J., Takahashi, M., Mon, H., Nakajima, Y., Koga, K., Kawaguchi, Y., Kusakabe, T., , Construction of gene expression systems in insect cell lines using promoters from the silkworm, Bombyx mori. , J. Biotechnol. , 133, 9-17., 2008.06.
37. Kawakami, N., Lee, J., Mon, H., Kubo, Y., Banno, Y., Kawaguchi, Y., Maenaka, K., Park, E.Y., Koga, K., Kusakabe, T., , Efficient Protein Expression in Bombyx mori Larvae of the Strain d17 Highly Sensitive to B. mori Nucleopolyhedrovirus., Mol. Biotechnol. , 40, 180-185., 2008.06.
38. Lee, J., Mon, H., Takahashi, M., Kawakami, N. Yoshida, Y., Banno, Y., Koga, K., Kawaguchi, Y., Kusakabe, T., , Screening of high-permissive silkworm strains for efficient recombinant protein production in Autographa californica nuclear polyhedrosis virus (AcNPV). , J. Insect Biotech. Seric., 76, 101-106., 2007.06.
39. Yamashita, J., Miyagawa, Y., Sugahara, R., Mon, H., Mitsunobu, H., Lee, J., Kawaguchi, Y., and Kusakabe, T., , Molecular Cloning of Silkworm Cdc37 and its Interaction with Hsp90 Chaperon. , J. Insect Biotech. Seric., 76, 137-143., 2007.06.
40. Sugahara, R., Mon, H., Yamashita, J., Mitsunobu, H., Lee, J., Kawaguchi, Y., Koga, K., and Kusakabe, T.,, Heterotrimeric Complex of Replication Protein A, a Single-Stranded DNABinding Protein, from the Silkworm, Bombyx mori., J. Insect Biotech. Seric., 76, 129-135., 2007.06.
41. Takahashi M., Lee J., Mon H., Yoshida H., Kawaguchi Y., Maekawa H., Koga K., and Kusakabe T. , Radiation resistance and its inheritance in the silkworm, Bombyx mori., J. Fac. Agr., Kyushu Univ. , 51, 261-264., 2006.10.
42. Mitsunobu H., Sakashita K., Mon H., Lee J., Kawaguchi Y., Koga K., and Kusakabe T. , Construction of gateway-based destination vectors for detecting subcellular localization of proteins in the silkworm, Bombyx mori. , J. Insect Biotech. Seric. , 75, 141-146., 2006.10.
43. Tsukioka H., Takahashi M., Mon H., Okano K., Mita K., Shimada T., Lee J., Kawaguchi Y., Koga K., and Kusakabe T. , Role of the Silkworm Argonaute2 Homologue Gene in Double-Strand Break Repair of Extrachromosomal DNA. , Nucleic Acids Res. , 34. 1092-1101., 2006.02.
44. Nakayama G., Kawaguchi Y., Koga K., and Kusakabe T. , Site-specific gene integration in cultured silkworm cells mediated by φC31 integrase., Mol. Genet. Genomics , 275, 1-8., 2006.01.
45. Kawaguchi, Y., Doira, H., Ohta, K., Kusakabe, T., and Koga, K., Inheritance of gon, a body color mutation of newly hatched larvae in Bombyx mori., J. Insect Biotech. Seric., 74, 35-37., 2005.01.
46. Chieda,Y., Iiyama,K., Yasunaga-Aoki, C., Lee, J., Kusakabe, T., Shimizu, S., Pathogenicity of gacA mutant of Pseudomonas aeruginosa PA01 in the silkworm, Bombyx mori., FEMS Microbiol. Lett., 10.1016/j.femsle.2005.01.032, 244, 1, 181-186, 244, 181?186., 2005.01.
47. Shinohara H., Iwasaki T., Miyazaki Y., Matsuo K., Aoki T., Matsumoto M., Oka T., Kurisaki J.I., Mizumachi K., Kusakabe T., Koga K., and Sugimoto Y., Thermostabilized ovalbumin that occurs naturally during development accumulates in embryonic tissues., Biochim. Biophys. Acta, 10.1016/j.bbagen.2005.02.016, 1723, 1-3, 106-113, 1723, 106-113., 2005.01.
48. Miyagawa Y., Lee J., Maeda T., Koga K. Kawaguchi Y., Kusakabe T., Differential expression of a Bombyx mori AHA1 homologue during spermatogenesis., Insect Mol. Biol., 10.1111/j.1365-2583.2004.00553.x, 14, 3, 245-253, 14, 245-253., 2005.01.
49. Maeda T., Kusakabe T., Lee J., Miyagawa Y., Kawaguchi Y., Koga K., Cloning and Characterization of a Ribonuclease L Inhibitor from the Silkworm, Bombyx mori., DNA Sequence, 10.1080/10425170400028871, 16, 1, 21-27, 16, 21-27., 2005.01.
50. Ohsaki A., Iiyama K., Miyagawa Y., Kawaguchi Y., Koga K., and Kusakabe T., Nonhomologous end-joining in a cell-free extract from the cultured silkworm cell line BmN4., Mol. Biol. Reports, 10.1007/s11033-004-2474-y, 32, 1, 25-34, 32, 25-34., 2005.01.
51. Lee J., Takahashi M., Mon H., Koga K., Kawaguchi Y., and Kusakabe T., Efficient gene transfer into silkworm larval tissues by a combination of sonoporation and lipofection., Cell Biol. Int., 10.1016/j.cellbi.2005.07.007, 29, 11, 976-979, 2005.01.
52. Lee J., Kusakabe T., Kawaguchi Y., Takahashi M., Mon H., Nho S., and Koga K., Molecular cloning and characterization of the translationally controlled tumor protein (TCTP) gene in Bombyx mori., Comp. Biochem. Phys., 10.1016/j.cbpc.2004.06.004, 139, 1, 35-43, 139, 35-43., 2004.01.
53. Mon H., Kusakabe, T., Lee, J., Kawaguch, Y., and Koga, K., A method for measuring the efficiency of gene targeting in cultured silkworm cells., Comp. Biochem. Phys., 139, 99-106., 2004.01.
54. Mon, H., Kusakabe, T., Bando, H., Kojima, K., Kawaguch, Y., and Koga, K., Analysis of extrachromosomal homologous recombination in cultured silkworm cells, Biochem. Biophys. Res. Commun., 10.1016/j.bbrc.2003.10.169, 312, 3, 684-690, 312, 684-690., 2003.12.
55. Kawaguchi, Y., Kusakabe, T., and Koga, K., Influence of N-metyl-N-nitrosourea treatment on embryogenesis of Bombyx mori, J. Fac. Agr., Kyushu Univ., 48, 1-2, 59-64, 48, 59-64., 2003.09.
56. Kawaguchi, Y., Ichida, M., Kusakabe, T., and Koga, K., Chorion architecture in the Japanese giant silkmoth, Caligula japonica japonica moore, Sericologia, 43, 29-39., 2003.06.
57. Fujikawa, T., Kawaguchi, Y., Kusakabe, T., and Koga, K., Histological studies on the yolk granule formation in the egg character mutant, vit, of Bombyx mori, J. Insect Biotech. Seric., 72, 111-115., 2003.05.
58. Lee, J., Kusakabe, T., Kawaguchi, Y., Aoki, C., Nho, S., and Koga, K., Molecular characterization of a Heat Shock Cognate 70-4 promoter from the silkworm, Bombyx mori, J. Insect Biotech. Seric., 72, 33-39., 2003.02.
59. Ota, A., Kusakabe, T., Yasushi Sugimoto, Takahashi, T., Nakajima, Y., Y Kawaguchi, Y., and Koga, K., Cloning and characterization of testis-specific tektin in Bombyx mori, Comp. Biochem. Phys., 10.1016/S1096-4959(02)00153-7, 133, 3, 371-382, 133, 371-382., 2002.03.
60. Yamamoto, T., Fujii, H., Kusakabe, T., Koga, K., Aso, Y., and Ishiguro, M., Change in phenoloxidase and its precursor during silkworm (a80 strain) development., J. Fac. Agr., Kyushu Univ., 45, 2, 487-493, 45, 487-493., 2001.01.
61. Xia Q., Fujii H., Kusakabe T., and Banno Y., Identification of three annexin IX isoforms generated by alternative splicing of the carboxyl-terminal exon in silkworm, Bombyx mori., Insect Biochem. Mol. Biol., 10.1016/S0965-1748(01)00074-1, 32, 1, 9-14, 32, 9-14., 2001.01.
62. Miyano, M., Okabe, T., Kusakabe, T., and Ohyama, T., Effect of upstream DNA architecture on transcription of a human LINE-1., J. Adv. Sci., 13, 1-6., 2001.01.
63. Tone, S., Kubo, T., Ohyama, T., Kusakabe, T., and Minatogawa, Y., Quantitation of mRNA using in vitro RNA amplification and Northern hybridization., Anal. Biochem., 10.1006/abio.2000.4745, 284, 2, 420-422, 284, 420-422., 2000.01.
64. Kawaguchi, Y., Kusakabe, T., Banno, Y., and Koga, K., Effect of ooplasmic size on the larval development as observed in the small egg mutant emi of Bombyx mori., J. Sericult. Sci. JPN., 68, 245-250., 1999.01.
65. Kawaguchi, Y., Kusakabe, T., and Koga, K., Unique chorion structure of the mottled gray (mgr) egg, an egg-character mutation of Bombyx mori (Lepidoptera: Bombycidae)., Appl. Entomol. Zool., 34, 3, 359-364, 34, 359-364., 1999.01.
66. Kusakabe, T., Baradaran, K., Lee, J., and Richardson, C. C., Roles of the helicase and primase domain of the gene 4 protein of bacteriophage T7 in accessing primase recognition site., EMBO J., 17, 1542-1552., 1998.01.
67. Lee, J., Chastain, P. D., Kusakabe, T., Griffith, J. D., and Richardson, C. C., Coordinated leading and lagging strand DNA synthesis on a mini-circular template., Mol. Cell, 1, 1001-1010., 1998.01.
68. Kusakabe, T., and Richardson, C. C., Template recognition and ribonucleotide specificity of the DNA primase of bacteriophage T7., J. Biol. Chem., 272, 5943-5951., 1997.01.
69. Inoue, T., Yatsuki, H., Kusakabe, T., Joh, K., Takasaki, Y., Nikoh, N., Miyata, T., and Hori, K., Caenorhabditis elegans has two isozymic forms, CE-1 and CE-2, of fructose-1,6-bisphosphate aldolases which are encoded by different genes., Arch. Biochem. Biophys., 339, 226-234., 1997.01.
70. Kusakabe, T., and Richardson, C. C., Gene 4 DNA primase of bacteriophage T7 mediates the annealing and extension of oligonucleotides at primase recognition sites., J. Biol. Chem., 272, 12446-12453., 1997.01.
71. Kusakabe, T., Motoki, K., and Hori, K., Mode of interactions of human aldolase isozymes with cytoskeletons., Arch. Biochem. Biophys., 344, 184-193., 1997.01.
72. Kuba, M, Yatsuki, H., Kusakabe. T., Takasaki. Y., Nikoh. N., Miyata. T., Yamaguchi. T., and Hori, K., Molecular evolution of amphioxus fructose-1,6-bisphosphate aldolases., Arch. Biochem. Biophys., 348, 329-336., 1997.01.
73. Kaihara, R., Matsuhashi, S., Kusakabe, T., Kondo, T., Iwanaga, A., and Hori, K., Monoclonal anti-human aldolase C antibodies that react to the isozyme group-specific sequences and generally conserved sequences of human aldolase C., J. Biochem., 119, 281-290., 1996.01.
74. Sugimoto, Y., Kusakabe, T., Kai, T., Okamura, T., Koga, K., and Hori, K., Analysis of the in vitro translation products of a novel type Drosophila melanogaster aldolase mRNA in which two carboxyl-terminal exon remain unspliced., Arch. Biochem. Biophys., 10.1006/abbi.1995.9953, 323, 2, 361-366, 323, 361-366, 1995.01.
75. Kusakabe, T., Motoki, K., Sugimoto, Y., Takasaki, Y., and Hori, K., Human aldolase B: Liver-specific properties of the isozyme depend on type-B isozyme group-specific sequences., Protein Enginieer., 7, 1387-1393., 1994.01.
Presentations
 Show All Presentations >>
1. 門 宏明, JAE MAN LEE, takahiro kusakabe, Novel kinetochore protein complex from silkworm holocentric chromosomes, 29th Annual Symposiumof the Protein Society, 2015.07.
2. 季明明, 李　在萬, 門 宏明, 李志清, 徐剣, 祝力, 日下部 宜宏, Lipidation, delipidation and degradation of Atg8 in Bombyx cells, 日本蚕糸学会, 2016.03.
3. Jian Xu, 門 宏明, JAE MAN LEE, takahiro kusakabe, RNA-Seq-based transcriptome analysis of p38, ERK and JNK MAPK gene suppressions in cultured Bombyx mori cells
, 日本分子生物学会, 2015.12.
4. Masato Hino, JAE MAN LEE, Mami Yamashita, Kazuma Hirata, Daisuke Morokuma, 門 宏明, takahiro kusakabe, Expression and Purification of IL-1brelated proteins Using Silkworm Baculovirus Protein Expression System
, International Symposium on Basic and Applied Research on Sericulture and Insect Sciences, 2015.04.
5. Masato Hino, Takumi Mitsudome, 門 宏明, Masanao Sato, Yoshitaka Suetsugu, Kimiko Yamamoto, Zhiqing Li, Jian Xu, Li Zhu, JAE MAN LEE, takahiro kusakabe, DNA methyltransferase DNMT-1 from Bombyx mori, International Symposium on Basic and Applied Research on Sericulture and Insect Sciences, 2015.04.
6. Kazuma Hirata, JAE MAN LEE, Jian Xu, Masato Hino, Mami Yamashita, 門 宏明, takahiro kusakabe, Expression and Purification of Fibroblast Growth Factors 1, 7, and 10 from Silkworm-Baculovirus Expression Vector System, International Symposium on Basic and Applied Research on Sericulture and Insect Sciences, 2015.04.
7. Daisuke Morokuma, 門 宏明, JAE MAN LEE, takahiro kusakabe, Expression, purification, and characterization of human alpha 1 acid glycoprotein (α1AGP) for glycobiological research in baculovirus expression system, International Symposium on Basic and Applied Research on Sericulture and Insect Sciences, 2015.04.
8. Daisuke Morokuma, 門 宏明, JAE MAN LEE, takahiro kusakabe, YUTAKA BANNO, Expression and characterization of short-type human alpha 1 acid glycoprotein (α1AGP∆) for glycobiological research in baculovirus expression system, International Symposium on Basic and Applied Research on Sericulture and Insect Sciences, 2015.04.
9. Kazuma Hirata, Atsushi Masuda, 門 宏明, JAE MAN LEE, takahiro kusakabe, Efficient Endo H expression by silkworm baculovirys expression system, International Symposium on Basic and Applied Research on Sericulture and Insect Sciences, 2015.04.
10. Mami Yamashita, Jian Xu, 門 宏明, JAE MAN LEE, takahiro kusakabe, Improvement of Caenorhabditis elegans SID-1-Dependent DNA Delivery System in Cultured Silkworm Cells, International Symposium on Basic and Applied Research on Sericulture and Insect Sciences, 2015.04.
11. Mami Yamashita, Masato Hino, Kazuma Hirata, Jian Xu, Mingming Ji, Daisuke Morokuma, 門 宏明, JAE MAN LEE, takahiro kusakabe, Expression, purification, and characterization of streptavidin mutants using Baculovirus-mediated silkworm protein expression system, International Symposium on Basic and Applied Research on Sericulture and Insect Sciences, 2015.04.
12. Masato Hino, 門 宏明, Li Zhu, Mami Yamashita, Kazuma Hirata, Jian Xu, Mingming Ji, Dan Zheng, Daisuke Morokuma, JAE MAN LEE, takahiro kusakabe, Characterization of silkworm replication related proteins for making an artificial chromosome, The 4th Asia-Pacific Congress of Sericulture and Insect Biotechnology, 2015.04.
13. Mami Yamashita, Masato Hino, Kazuma Hirata, Jian Xu, Mingming Ji, Daisuke Morokuma, 門 宏明, JAE MAN LEE, takahiro kusakabe, Characterization of BmNPV Promoters for Improving Exogenous Genes Expression in Bombyx Cells, The 4th Asia-Pacific Congress of Sericulture and Insect Biotechnology, 2015.04.
14. Kazuma Hirata, Jian Xu, 門 宏明, JAE MAN LEE, takahiro kusakabe, Masato Hino, Mami Yamashita, Establishiment of New insect Cell Lines by Using Cell Fusion Method, The 4th Asia-Pacific Congress of Sericulture and Insect Biotechnology, 2015.04.
15. Daisuke Morokuma, Jian Xu, 門 宏明, JAE MAN LEE, takahiro kusakabe, Synthesis of Complex-Type N-Glycans on Recombinant Proteins by Modifying N-Glycosylation Pathway in Cultured Silkworm Cells, The 4th Asia-Pacific Congress of Sericulture and Insect Biotechnology, 2015.04.
16. Ming-Ming Ji, Jian Xu, Li Zhu, 門 宏明, JAE MAN LEE, takahiro kusakabe, Zhiqing Li, GFP-p62 degradation is an excellent marker of autophagic flux in Bombyx, The 4th Asia-Pacific Congress of Sericulture and Insect Biotechnology, 2015.04.
17. Jian Xu, Jianping Chen, Li Zhu, 門 宏明, JAE MAN LEE, takahiro kusakabe, Engineering of silkworm-baculovirus expression system for efficient production of G protein -coupled receptors, The 4th Asia-Pacific Congress of Sericulture and Insect Biotechnology, 2015.04.
18. 日下部 宜宏, 李　在萬, 門 宏明, 徐剣, 祝力, 李志清, Role of silkworm RISC components in the basal defense against Bombyx mori nucleopolyhedrovirus (BmNPV), 日本蚕糸学会, 2014.03.


Unauthorized reprint of the contents of this database is prohibited.

Copyright © 2006, Kyushu University. All rights reserved.