Kyushu University Academic Staff Educational and Research Activities Database
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takahiro kusakabe Last modified date:2018.06.08

Professor / Agricultural Bioresource Sciences
Department of Bioresource Sciences
Faculty of Agriculture


Graduate School
Undergraduate School


Academic Degree
Ph.D
Field of Specialization
Insect Molecular Genetics
Research
Research Interests
  • Analysis of DNA damage and homologous recombination repair in insect cells
    keyword : Silkworm, Homologous recombination, DSB, DNA repair
    1999.04A double-strand break (DSB) is caused by a variety of exogenous and endogenous DNA damaging agents, including ionizing radiation and free radicals. If damage is left unrepaired or is misrepaired, it may lead cells to genetic disorder or cell death. Therefore, precise repair of DSBs is essential for maintaining the viability and genomic integrity of cells. All organisms, from bacteria to insects, and mammals, evolved to have at least two pathways for repairing DSBs; homologous recombination (HR) and non-homologous end joining (NHEJ). HR ensures accurate DSB repair using genetic information from an intact chromosome as a template. At the present study, HR repair system in insect cells is analyzed by using transgenic silkworm. Silkworm, Bombyx mori, has many unique characters related to HR. For example, female silkworm lacks meiotic recombination, but not male. The DNA repair system of an organism's genome is an extraordinarily complex process whereby a multiple of synchronized proteins reproduce the genetic material accurately as well as possible after damage. Thus, the silkworm is one of the most useful and interesting model insect for studying HR systems in molecular level because the whole genome sequence of silkworm was disclosed in this year, and more than 90,000 ESTs are available from public databases. We had already developed the sensitive assay systems for HR in cultured silkworm cells. As a next study, we plan to establish the similar systems in transgenic insects, and using these systems, we would like to reveal whether DSBs were repaired by tissue- or stage-specific manner after ionizing irradiation or artifical enzymatic induction of DSBs. In these processes, we particularly focus on the roles of HR-related genes in tissue- or stage-specific HR pathways by assessing the effects of gene knockdown using RNA interference methods. I hope that the results obtained from this proposal would make a great contribution to our understanding for DNA repair system and to application for genomic drug discovery and biotechnology.
Academic Activities
Papers
1. takahiro kusakabe, Proteasome inhibitor MG132 impairs autophagic flux through compromising formation of autophagosomes in Bombyx cells, 479, 690-696, 2016.11.
2. takahiro kusakabe, Characterization of the roles of DNA polymerases, clamp, and clamp loaders during S-phase progression and cell cycle regulation in the silkworm, Bombyx mori, 85, 21-29, 2016.06.
3. takahiro kusakabe, Comparative proteomic analysis of hemolymph proteins from Autographa californica multiple nucleopolyhedrovirus (AcMNPV)-sensitive or -resistant silkworm strains during infections, COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS, 10.1016/j.cbd.2015.07.003, 16, 36-47, 2015.12.
4. takahiro kusakabe, Roles of silkworm endoplasmic reticulum chaperones in the secretion of recombinant proteins expressed by baculovirus system, MOLECULAR AND CELLULAR BIOCHEMISTRY, 10.1007/s11010-015-2529-5, 409, 1-2, 255-262, 2015.11.
5. Li Zhu, Tsuneyuki Tatsuke, Jian Xu, Zhiqing Li, 門 宏明, JAE MAN LEE, takahiro kusakabe, Loqs depends on R2D2 to localize in D2 body-like granules and functions in RNAi pathways in silkworm cells, 64, 78-90, 2015.09.
6. takahiro kusakabe, Mass Production of an Active Peptide-N-Glycosidase F Using Silkworm-Baculovirus Expression System, MOLECULAR BIOTECHNOLOGY, 10.1007/s12033-015-9866-1, 57, 8, 735-745, 2015.08.
7. takahiro kusakabe, Human alpha 1-acid glycoprotein as a model protein for glycoanalysis in baculovirus expression vector system, JOURNAL OF ASIA-PACIFIC ENTOMOLOGY, 10.1016/j.aspen.2015.03.006, 18, 2, 303-309, 2015.06.
8. takahiro kusakabe, Improvement of Endo-β-N-acetylglucosaminidase H production using silkworm–baculovirus protein expression system, Journal of Asia-Pacific Entomology, 18, 2, 175-180, 2015.06.
9. Daisuke Morokuma, 門 宏明, YUTAKA BANNO, takahiro kusakabe, JAE MAN LEE, Differential N-Glycan Modifications of Human Alpha 1-Acid Glycoprotein (α1AGP) Produced in Different Silkworm Strains using the Baculovirus Expression System, 84, 2, 49-53, 2016.05.
10. 日下部 宜宏, Soaking RNAi-mediated modification of Sf9 cells for baculovirus expression system by ectopic expression of Caenorhabditis elegans SID-1, 10.1007/s00253-013-4785-1, 97, 13, 5921-5931, 2013.07.
11. Zhu, L., Tatsuke, T., Li, Z., Mon, H., Xu, Z., Lee, J., and Kusakabe T.,, Molecular cloning of BmTUDOR-SN and analysis of its role in RNAi pathway in the silkworm, Bombyx mori (Lepidoptera: Bombycidae). , Appl. Entomol. Zool., 2012.06.
12. Sugahara, R., Mon, H., Lee, J., and Kusakabe, T.,, Monoubiquitination-Dependent Chromatin Loading of FancD2 in Silkworms, a Species Lacking the FA Core Complex., Gene, 2012.05.
13. Lee, J., Mon, H., Banno, Y., Iiyama, K., Kusakabe, T., Bombyx mori strains useful for efficient recombinant protein production using a baculovirus vector., J. Biotech. Biomater., 2012.06.
14. Fujii, M., Takahashi, M., Mon, H., Tatsuke, T., Lee, J., and Kuskabe T., Molecular cloning of the silkworm p53R2 homolog gene. , J. Fac. Agr. Kyushu Univ., , 57, 79-82, 2012.04.
15. Nagata, Y., Sakashita, K., Imanishi, S., Lee, J., and Kuskabe T. , Expression of glycosylated mucin-like domain using baculovirus expression system in silkworm, Bombyx mori., J. Fac. Agr. Kyushu Univ., , 57, 83-86, 2012.04.
16. Li, Z., Cheng, D., Mon, H., Tatsuke, T., Zhu, L., Xu, J., Lee, J., Xia, Q., and Kusakabe, T., Genome-wide identification of Polycomb target genes reveals a functional association of Pho with Scm in Bombyx mori., PLoS ONE, , 7, e34330, 2012.04.
17. Mon, H., Kobayashi, I., Ohkubo, S. Tomita, S., Lee, J., Sezutsu, H., Tamura, T., Kusakabe, T., Effective RNA interference in cultured silkworm cells mediated by overexpression of Caenorhabditis elegans SID-1., RNA Biol., , 9, 40-46, 2012.01.
18. Mitsunobu, H., Izumi, H., Mon, H., Tatsuke, T., Lee, J., Kusakabe, T., Molecular Characterization of heterochromatin proteins 1a and 1b from the Silkworm, Bombyx mori., Insect Mol. Biol. , 21, 9-20, 2012.01.
19. Li, Z., Tatsuke, T., Sakashita, K., Zhu, L., Xu, J., Mon, H., Lee, J., and Kusakabe, T., Identification and characterization of Polycomb group genes in the silkworm, Bombyx mori., Mol. Biol. Report, , 39, 5575-5588, 2012.01.
20. Mon, H., Izumi, M., Mitsunobu, H., Tatsuke, T., Iiyama, K., Jikuya, H., Lee, J., Kusakabe, T., Post-translational modifications of the N-terminal tail of histone H3 in holocentric chromosomes of bombyx mori. Insect Biochem. , Mol. Genet. Genomics, 2011.09.
21. Mon, H., Lee, J., Kawaguchi, Y., Kusakabe, T., Double-strand breaks repair by gene conversion in silkworm holocentric chromosomes, Mol. Genet. Genomics, 2011.09.
22. Mitsunobu, H., Izumi, H., Mon, H., Tatsuke, T., Lee, J., Kusakabe, T., Molecular Characterization of heterochromatin proteins 1a and 1b from the Silkworm, Bombyx mori. , Insect Mol. Biol. , 2011.04.
23. Mitsunobu, H., Izumi, M., Iiyama, K., Jikuya, H., Lee, J., Mon, H., Kawaguchi, Y., Kusakabe, T. , Molecular Characterization of Core Histones in the Silkworm, Bombyx mori. , J. Insect Biotech. Seric., 79, 75-83, 2010.10.
24. Hong, S.M., Yamashita, J., Mitsunobu, H., Uchino, K., Kobayashi, I., Tamura, T., Nakajima, H., Miyagawa, H., Lee, J.M., Mon, H., Miyata, T., Kawaguchi, Y., Kusakabe, T., Efficient Soluble Protein Production on Transgenic Silkworms Expressing Cytoplasmic Chaperones using Baculovirus Expression System. , Appl. Microbiol. Biotech, 2010.04.
25. Tatsuke, T., Hong, S.M., Tobata, H., Mon, H., Lee, J., Kawaguchi, Y., Kusakabe, T., Construction of piggyBac-based Vectors Using Visible and Drug-resistance Marker for Introducing Foreign Genes into Silkworm Cultured Cells. , J. Fac. Agr., Kyushu Univ. , 54, 397-400, 2009.04.
26. Sakashita, K., Tatsuke, T., Masaki, Y., Lee, J., Kawaguchi, Y., Kusakabe, T. , dsRNA Binding Activity of Silkworm Larval Hemolymph is Mediated by Lipophorin Complex , J. Fac. Agr., Kyushu Univ. , 54, 401-406, 2009.04.
27. Mon, H., Sugahara, R., Hong, S.M., Lee, J., Kamachi, Y., Kawaguchi, Y., Kusakabe, T. , Analysis of protein interactions with two-hybrid system in cultured insect cells. , Anal. Biochem. , 392, 180-182., 2009.09.
28. Hong, S.M., Noh, S.K., Kim, K.A., Mitsunobu, H., Mon, H., Lee, J.M., Kawaguchi, Y., Kusakabe, T. , Molecular Characterization, Localization, and Distribution of Innexins in the Silkworm, Bombyx mori. , Mol. Biotechnol. , 43, 52-58., 2009.09.
29. Sakashita, K., Tatsuke, T., Lee, J., Kawaguchi, Y., and Kusakabe, T. , Sequence-Nonspecific Suppression of Gene Expression by Double-stranded RNA in Silkworm Cultured Cells. , J. Insect Biotech. Seric. , 78, 33-37., 2009.06.
30. Karasaki, N., Mon, H., Takahashi, M., Lee, J., Koga, K., Kawaguchi, Y., Kusakabe, T. , Establishment of Tetracycline-inducible Gene Expression Systems in the Silkworm, Bombyx mori. , Biotechnol. Lett. , 31, 495-500., 2009.06.
31. Kawakami, N., Lee, J., Mon, H., Kubo, Y., Banno, Y., Kawaguchi, Y., Maenaka, K., Park, E.Y., Koga, K., Kusakabe, T., , Efficient Protein Expression in Bombyx mori Larvae of the Strain d17 Highly Sensitive to B. mori Nucleopolyhedrovirus., Mol. Biotechnol. , 40, 180-185., 2008.06.
32. Lee, J., Takahashi, M., Mon, H., Nakajima, Y., Koga, K., Kawaguchi, Y., Kusakabe, T., , Construction of gene expression systems in insect cell lines using promoters from the silkworm, Bombyx mori. , J. Biotechnol. , 133, 9-17., 2008.06.
33. Sugahara, R., Mon, H., Yamashita, J., Mitsunobu, H., Lee, J., Kawaguchi, Y., Koga, K., and Kusakabe, T.,, Heterotrimeric Complex of Replication Protein A, a Single-Stranded DNABinding Protein, from the Silkworm, Bombyx mori., J. Insect Biotech. Seric., 76, 129-135., 2007.06.
34. Yamashita, J., Miyagawa, Y., Sugahara, R., Mon, H., Mitsunobu, H., Lee, J., Kawaguchi, Y., and Kusakabe, T., , Molecular Cloning of Silkworm Cdc37 and its Interaction with Hsp90 Chaperon. , J. Insect Biotech. Seric., 76, 137-143., 2007.06.
35. Lee, J., Mon, H., Takahashi, M., Kawakami, N. Yoshida, Y., Banno, Y., Koga, K., Kawaguchi, Y., Kusakabe, T., , Screening of high-permissive silkworm strains for efficient recombinant protein production in Autographa californica nuclear polyhedrosis virus (AcNPV). , J. Insect Biotech. Seric., 76, 101-106., 2007.06.
36. Mitsunobu H., Sakashita K., Mon H., Lee J., Kawaguchi Y., Koga K., and Kusakabe T. , Construction of gateway-based destination vectors for detecting subcellular localization of proteins in the silkworm, Bombyx mori. , J. Insect Biotech. Seric. , 75, 141-146., 2006.10.
37. Takahashi M., Lee J., Mon H., Yoshida H., Kawaguchi Y., Maekawa H., Koga K., and Kusakabe T. , Radiation resistance and its inheritance in the silkworm, Bombyx mori., J. Fac. Agr., Kyushu Univ. , 51, 261-264., 2006.10.
38. Tsukioka H., Takahashi M., Mon H., Okano K., Mita K., Shimada T., Lee J., Kawaguchi Y., Koga K., and Kusakabe T. , Role of the Silkworm Argonaute2 Homologue Gene in Double-Strand Break Repair of Extrachromosomal DNA. , Nucleic Acids Res. , 34. 1092-1101., 2006.02.
39. Nakayama G., Kawaguchi Y., Koga K., and Kusakabe T. , Site-specific gene integration in cultured silkworm cells mediated by φC31 integrase., Mol. Genet. Genomics , 275, 1-8., 2006.01.
40. Lee J., Takahashi M., Mon H., Koga K., Kawaguchi Y., and Kusakabe T., Efficient gene transfer into silkworm larval tissues by a combination of sonoporation and lipofection., Cell Biol. Int., 10.1016/j.cellbi.2005.07.007, 29, 11, 976-979, 2005.01.
41. Ohsaki A., Iiyama K., Miyagawa Y., Kawaguchi Y., Koga K., and Kusakabe T., Nonhomologous end-joining in a cell-free extract from the cultured silkworm cell line BmN4., Mol. Biol. Reports, 10.1007/s11033-004-2474-y, 32, 1, 25-34, 32, 25-34., 2005.01.
42. Maeda T., Kusakabe T., Lee J., Miyagawa Y., Kawaguchi Y., Koga K., Cloning and Characterization of a Ribonuclease L Inhibitor from the Silkworm, Bombyx mori., DNA Sequence, 10.1080/10425170400028871, 16, 1, 21-27, 16, 21-27., 2005.01.
43. Miyagawa Y., Lee J., Maeda T., Koga K. Kawaguchi Y., Kusakabe T., Differential expression of a Bombyx mori AHA1 homologue during spermatogenesis., Insect Mol. Biol., 10.1111/j.1365-2583.2004.00553.x, 14, 3, 245-253, 14, 245-253., 2005.01.
44. Shinohara H., Iwasaki T., Miyazaki Y., Matsuo K., Aoki T., Matsumoto M., Oka T., Kurisaki J.I., Mizumachi K., Kusakabe T., Koga K., and Sugimoto Y., Thermostabilized ovalbumin that occurs naturally during development accumulates in embryonic tissues., Biochim. Biophys. Acta, 10.1016/j.bbagen.2005.02.016, 1723, 1-3, 106-113, 1723, 106-113., 2005.01.
45. Chieda,Y., Iiyama,K., Yasunaga-Aoki, C., Lee, J., Kusakabe, T., Shimizu, S., Pathogenicity of gacA mutant of Pseudomonas aeruginosa PA01 in the silkworm, Bombyx mori., FEMS Microbiol. Lett., 10.1016/j.femsle.2005.01.032, 244, 1, 181-186, 244, 181?186., 2005.01.
46. Kawaguchi, Y., Doira, H., Ohta, K., Kusakabe, T., and Koga, K., Inheritance of gon, a body color mutation of newly hatched larvae in Bombyx mori., J. Insect Biotech. Seric., 74, 35-37., 2005.01.
47. Mon H., Kusakabe, T., Lee, J., Kawaguch, Y., and Koga, K., A method for measuring the efficiency of gene targeting in cultured silkworm cells., Comp. Biochem. Phys., 139, 99-106., 2004.01.
48. Lee J., Kusakabe T., Kawaguchi Y., Takahashi M., Mon H., Nho S., and Koga K., Molecular cloning and characterization of the translationally controlled tumor protein (TCTP) gene in Bombyx mori., Comp. Biochem. Phys., 10.1016/j.cbpc.2004.06.004, 139, 1, 35-43, 139, 35-43., 2004.01.
49. Mon, H., Kusakabe, T., Bando, H., Kojima, K., Kawaguch, Y., and Koga, K., Analysis of extrachromosomal homologous recombination in cultured silkworm cells, Biochem. Biophys. Res. Commun., 10.1016/j.bbrc.2003.10.169, 312, 3, 684-690, 312, 684-690., 2003.12.
50. Kawaguchi, Y., Ichida, M., Kusakabe, T., and Koga, K., Chorion architecture in the Japanese giant silkmoth, Caligula japonica japonica moore, Sericologia, 43, 29-39., 2003.06.
51. Kawaguchi, Y., Kusakabe, T., and Koga, K., Influence of N-metyl-N-nitrosourea treatment on embryogenesis of Bombyx mori, J. Fac. Agr., Kyushu Univ., 48, 1-2, 59-64, 48, 59-64., 2003.09.
52. Fujikawa, T., Kawaguchi, Y., Kusakabe, T., and Koga, K., Histological studies on the yolk granule formation in the egg character mutant, vit, of Bombyx mori, J. Insect Biotech. Seric., 72, 111-115., 2003.05.
53. Lee, J., Kusakabe, T., Kawaguchi, Y., Aoki, C., Nho, S., and Koga, K., Molecular characterization of a Heat Shock Cognate 70-4 promoter from the silkworm, Bombyx mori, J. Insect Biotech. Seric., 72, 33-39., 2003.02.
54. Ota, A., Kusakabe, T., Yasushi Sugimoto, Takahashi, T., Nakajima, Y., Y Kawaguchi, Y., and Koga, K., Cloning and characterization of testis-specific tektin in Bombyx mori, Comp. Biochem. Phys., 10.1016/S1096-4959(02)00153-7, 133, 3, 371-382, 133, 371-382., 2002.03.
55. Miyano, M., Okabe, T., Kusakabe, T., and Ohyama, T., Effect of upstream DNA architecture on transcription of a human LINE-1., J. Adv. Sci., 13, 1-6., 2001.01.
56. Xia Q., Fujii H., Kusakabe T., and Banno Y., Identification of three annexin IX isoforms generated by alternative splicing of the carboxyl-terminal exon in silkworm, Bombyx mori., Insect Biochem. Mol. Biol., 10.1016/S0965-1748(01)00074-1, 32, 1, 9-14, 32, 9-14., 2001.01.
57. Yamamoto, T., Fujii, H., Kusakabe, T., Koga, K., Aso, Y., and Ishiguro, M., Change in phenoloxidase and its precursor during silkworm (a80 strain) development., J. Fac. Agr., Kyushu Univ., 45, 2, 487-493, 45, 487-493., 2001.01.
58. Tone, S., Kubo, T., Ohyama, T., Kusakabe, T., and Minatogawa, Y., Quantitation of mRNA using in vitro RNA amplification and Northern hybridization., Anal. Biochem., 10.1006/abio.2000.4745, 284, 2, 420-422, 284, 420-422., 2000.01.
59. Kawaguchi, Y., Kusakabe, T., and Koga, K., Unique chorion structure of the mottled gray (mgr) egg, an egg-character mutation of Bombyx mori (Lepidoptera: Bombycidae)., Appl. Entomol. Zool., 34, 3, 359-364, 34, 359-364., 1999.01.
60. Kawaguchi, Y., Kusakabe, T., Banno, Y., and Koga, K., Effect of ooplasmic size on the larval development as observed in the small egg mutant emi of Bombyx mori., J. Sericult. Sci. JPN., 68, 245-250., 1999.01.
61. Lee, J., Chastain, P. D., Kusakabe, T., Griffith, J. D., and Richardson, C. C., Coordinated leading and lagging strand DNA synthesis on a mini-circular template., Mol. Cell, 1, 1001-1010., 1998.01.
62. Kusakabe, T., Baradaran, K., Lee, J., and Richardson, C. C., Roles of the helicase and primase domain of the gene 4 protein of bacteriophage T7 in accessing primase recognition site., EMBO J., 17, 1542-1552., 1998.01.
63. Kuba, M, Yatsuki, H., Kusakabe. T., Takasaki. Y., Nikoh. N., Miyata. T., Yamaguchi. T., and Hori, K., Molecular evolution of amphioxus fructose-1,6-bisphosphate aldolases., Arch. Biochem. Biophys., 348, 329-336., 1997.01.
64. Kusakabe, T., Motoki, K., and Hori, K., Mode of interactions of human aldolase isozymes with cytoskeletons., Arch. Biochem. Biophys., 344, 184-193., 1997.01.
65. Kusakabe, T., and Richardson, C. C., Gene 4 DNA primase of bacteriophage T7 mediates the annealing and extension of oligonucleotides at primase recognition sites., J. Biol. Chem., 272, 12446-12453., 1997.01.
66. Inoue, T., Yatsuki, H., Kusakabe, T., Joh, K., Takasaki, Y., Nikoh, N., Miyata, T., and Hori, K., Caenorhabditis elegans has two isozymic forms, CE-1 and CE-2, of fructose-1,6-bisphosphate aldolases which are encoded by different genes., Arch. Biochem. Biophys., 339, 226-234., 1997.01.
67. Kusakabe, T., and Richardson, C. C., Template recognition and ribonucleotide specificity of the DNA primase of bacteriophage T7., J. Biol. Chem., 272, 5943-5951., 1997.01.
68. Kaihara, R., Matsuhashi, S., Kusakabe, T., Kondo, T., Iwanaga, A., and Hori, K., Monoclonal anti-human aldolase C antibodies that react to the isozyme group-specific sequences and generally conserved sequences of human aldolase C., J. Biochem., 119, 281-290., 1996.01.
69. Sugimoto, Y., Kusakabe, T., Kai, T., Okamura, T., Koga, K., and Hori, K., Analysis of the in vitro translation products of a novel type Drosophila melanogaster aldolase mRNA in which two carboxyl-terminal exon remain unspliced., Arch. Biochem. Biophys., 10.1006/abbi.1995.9953, 323, 2, 361-366, 323, 361-366, 1995.01.
70. Kusakabe, T., Motoki, K., Sugimoto, Y., Takasaki, Y., and Hori, K., Human aldolase B: Liver-specific properties of the isozyme depend on type-B isozyme group-specific sequences., Protein Enginieer., 7, 1387-1393., 1994.01.
Presentations
1. 門 宏明, JAE MAN LEE, takahiro kusakabe, Novel kinetochore protein complex from silkworm holocentric chromosomes, 29th Annual Symposiumof the Protein Society, 2015.07.
2. 季明明, 李 在萬, 門 宏明, 李志清, 徐剣, 祝力, 日下部 宜宏, Lipidation, delipidation and degradation of Atg8 in Bombyx cells, 日本蚕糸学会, 2016.03.
3. Jian Xu, 門 宏明, JAE MAN LEE, takahiro kusakabe, RNA-Seq-based transcriptome analysis of p38, ERK and JNK MAPK gene suppressions in cultured Bombyx mori cells
, 日本分子生物学会, 2015.12.
4. Masato Hino, JAE MAN LEE, Mami Yamashita, Kazuma Hirata, Daisuke Morokuma, 門 宏明, takahiro kusakabe, Expression and Purification of IL-1brelated proteins Using Silkworm Baculovirus Protein Expression System
, International Symposium on Basic and Applied Research on Sericulture and Insect Sciences, 2015.04.
5. Masato Hino, Takumi Mitsudome, 門 宏明, Masanao Sato, Yoshitaka Suetsugu, Kimiko Yamamoto, Zhiqing Li, Jian Xu, Li Zhu, JAE MAN LEE, takahiro kusakabe, DNA methyltransferase DNMT-1 from Bombyx mori, International Symposium on Basic and Applied Research on Sericulture and Insect Sciences, 2015.04.
6. Kazuma Hirata, JAE MAN LEE, Jian Xu, Masato Hino, Mami Yamashita, 門 宏明, takahiro kusakabe, Expression and Purification of Fibroblast Growth Factors 1, 7, and 10 from Silkworm-Baculovirus Expression Vector System, International Symposium on Basic and Applied Research on Sericulture and Insect Sciences, 2015.04.
7. Daisuke Morokuma, 門 宏明, JAE MAN LEE, takahiro kusakabe, Expression, purification, and characterization of human alpha 1 acid glycoprotein (α1AGP) for glycobiological research in baculovirus expression system, International Symposium on Basic and Applied Research on Sericulture and Insect Sciences, 2015.04.
8. Daisuke Morokuma, 門 宏明, JAE MAN LEE, takahiro kusakabe, YUTAKA BANNO, Expression and characterization of short-type human alpha 1 acid glycoprotein (α1AGP∆) for glycobiological research in baculovirus expression system, International Symposium on Basic and Applied Research on Sericulture and Insect Sciences, 2015.04.
9. Kazuma Hirata, Atsushi Masuda, 門 宏明, JAE MAN LEE, takahiro kusakabe, Efficient Endo H expression by silkworm baculovirys expression system, International Symposium on Basic and Applied Research on Sericulture and Insect Sciences, 2015.04.
10. Mami Yamashita, Jian Xu, 門 宏明, JAE MAN LEE, takahiro kusakabe, Improvement of Caenorhabditis elegans SID-1-Dependent DNA Delivery System in Cultured Silkworm Cells, International Symposium on Basic and Applied Research on Sericulture and Insect Sciences, 2015.04.
11. Mami Yamashita, Masato Hino, Kazuma Hirata, Jian Xu, Mingming Ji, Daisuke Morokuma, 門 宏明, JAE MAN LEE, takahiro kusakabe, Expression, purification, and characterization of streptavidin mutants using Baculovirus-mediated silkworm protein expression system, International Symposium on Basic and Applied Research on Sericulture and Insect Sciences, 2015.04.
12. Masato Hino, 門 宏明, Li Zhu, Mami Yamashita, Kazuma Hirata, Jian Xu, Mingming Ji, Dan Zheng, Daisuke Morokuma, JAE MAN LEE, takahiro kusakabe, Characterization of silkworm replication related proteins for making an artificial chromosome, The 4th Asia-Pacific Congress of Sericulture and Insect Biotechnology, 2015.04.
13. Mami Yamashita, Masato Hino, Kazuma Hirata, Jian Xu, Mingming Ji, Daisuke Morokuma, 門 宏明, JAE MAN LEE, takahiro kusakabe, Characterization of BmNPV Promoters for Improving Exogenous Genes Expression in Bombyx Cells, The 4th Asia-Pacific Congress of Sericulture and Insect Biotechnology, 2015.04.
14. Kazuma Hirata, Jian Xu, 門 宏明, JAE MAN LEE, takahiro kusakabe, Masato Hino, Mami Yamashita, Establishiment of New insect Cell Lines by Using Cell Fusion Method, The 4th Asia-Pacific Congress of Sericulture and Insect Biotechnology, 2015.04.
15. Daisuke Morokuma, Jian Xu, 門 宏明, JAE MAN LEE, takahiro kusakabe, Synthesis of Complex-Type N-Glycans on Recombinant Proteins by Modifying N-Glycosylation Pathway in Cultured Silkworm Cells, The 4th Asia-Pacific Congress of Sericulture and Insect Biotechnology, 2015.04.
16. Ming-Ming Ji, Jian Xu, Li Zhu, 門 宏明, JAE MAN LEE, takahiro kusakabe, Zhiqing Li, GFP-p62 degradation is an excellent marker of autophagic flux in Bombyx, The 4th Asia-Pacific Congress of Sericulture and Insect Biotechnology, 2015.04.
17. Jian Xu, Jianping Chen, Li Zhu, 門 宏明, JAE MAN LEE, takahiro kusakabe, Engineering of silkworm-baculovirus expression system for efficient production of G protein -coupled receptors, The 4th Asia-Pacific Congress of Sericulture and Insect Biotechnology, 2015.04.
18. 日下部 宜宏, 李 在萬, 門 宏明, 徐剣, 祝力, 李志清, Role of silkworm RISC components in the basal defense against Bombyx mori nucleopolyhedrovirus (BmNPV), 日本蚕糸学会, 2014.03.