| 1. |
Tomoya Noma, Hiroyuki Hamada, Taizo Hanai, Analysis of cell density-dependent gene expression systems for microbial chemical production, 日台韓化学工学会議, 2019.11. |
| 2. |
Taizo Hanai, Synthetic metabolic pathways in cyanobacteria for chemical production, 2018.11. |
| 3. |
Yuki Soma, Yuri Fujiwara, Kohsuke Hata, Yoshihiro Izumi, Takeshi Bamba, Taizo Hanai, Dynamic metabolic engineering harnessing synthetic biological tools and metabolome analysis, Metabolic Engineering 12, 2019.06. |
| 4. |
広川 安孝, Taizo Hanai, Engineering synthetic pathways in cyanobacteria for chemical production directly from carbon dioxide and light, iBioP2016, 2016.12. |
| 5. |
広川 安孝, Taizo Hanai, Photosynthetic chemical production by engineered cyanobacteria, YABEC2016, 2016.10. |
| 6. |
広川 安孝, Taizo Hanai, Iso-propanol production by engineered cyanobacteria, Synechococcus elongatus PCC 7942, Metabolic Engineering XI, 2016.06. |
| 7. |
Yuki Soma, Taizo Hanai, Synthetic quorum sensing as a tunable cell density sensor-regulator for dynamic metabolic engineering, Metabolic Engineering XI, 2016.06. |
| 8. |
Yuki Soma, Taizo Hanai, Autonomous gene expression by a tunable cell density sensor-regulator system for microbial isopropanol production, 「細胞を創る」研究会 「Japan - Korea Synthetic Biology Symposium」, 2015.11. |
| 9. |
Yuki Soma, Taizo Hanai, A Tunable Cell Density Sensor for Bioproduction, 日本生物工学会 国際シンポジウム「アジアにおける最新バイオリファイナリー研究」 "Recent advances in biorefinery research in Asian countries", 2015.10. |
| 10. |
Taizo Hanai, Application of Synthetic Biology to Bioproduction, Symposium of Biochemical Systems Theory, 2015.09. |
| 11. |
Yuki Soma, Keigo Tsuruno, Taizo Hanai, Synthetic Genetic Circuit for Improvement of iso-Propanol Production, KMB, 2015.06. |
| 12. |
Taizo Hanai, Synthetic Biology for application to bioproduction, SBJシンポジウム, 2015.05. |
| 13. |
Yuki Soma, Keigo Tsuruno, Taizo Hanai, Metabolic toggle switch for improvement of productivity in synthetic pathway, AOAIS (Asia-Oceania Algae Innovation Summit), 2014.11. |
| 14. |
Yuki Soma, Keigo Tsuruno, Taizo Hanai, Metabolic flux redirection for productivity improvement by metabolic toggle switch, iBio-T (Asian Symposium On Innovative BIO-Production and Biorefinery), 2014.11. |
| 15. |
Yuki Soma, Keigo Tsuruno, Masaru Wada, Atsushi Yokota, Taizo Hanai, Metabolic flux redirection from a central metabolic pathway toward a synthetic pathway using a metabolic toggle switch, Annual Meeting & Exhibition Society for Industrial Microbiology and Biotechnology, 2014.07. |
| 16. |
Yasutaka Hirokawa, Tamami Kusakabe, Tsuneyuki Tatsuke, Keigo Tsuruno, Shota Atsumi, James C. Liao, Taizo Hanai, Engineering a synthetic pathway in cyanobacteria for isopropanol production, International Conference on Algal Biomass, Biofuels and Bioproducts, 2014.06. |
| 17. |
Yuki Soma, Keigo Tsuruno, Taizo Hanai, Productivity Improvement in Synthetic Pathway by Metabolic Toggle Switch, UCLA workshop on metabolomics and metabolic engineering, 2014.06. |
| 18. |
Yuki Soma, Yohei Motomura, Mai Murakami, Shunsuke Yasutake, Keigo Tsuruno, Masahiro Okamoto, Taizo Hanai, Mathematical modeling and theoretical analysis for the quantitative control of the target gene expression of synthetic genetic circuit, CBI学会, 2013.10. |
| 19. |
Yuki Soma, Kentaro Inokuma, Tsutomu Tanaka, Akihiko Kondo, Chiaki Ogino, Taizo Hanai, Isopropanol production from cellobiose by E. coli, SIMB Annual Meeting, 2013.08. |
| 20. |
花井 泰三, Synthetic pathway for C3 or C4 alcohol production, Japan-UK meeting systems microbiology symposium, 2013.04. |
| 21. |
Taizo Hanai, Metabolic Engineering for isopropanol Production, IEcA(The International E. Coli Alliance), 2012.12, fficient bio-production from lignocellulosic biomass is required for the purpose of developing an inexpensive, practical bio-refinery process. As one approach to address this problem, we genetically engineered E. coli to produce isopropanol directly from cellobiose via the cellobiose degradation by Beta-Glucosidase (BGL) on the cell surface. We introduced the synthetic pathway for isopropanol production and the BGL protein from Thermobifida fusca YX (Tfu0937) fused to the anchor protein Blc (Tfu-Blc) into E. coli and compared their isopropanol production in the presence of cellobiose. This strain consumed cellobiose and produced 69.0±11.6 mM isopropanol at 21 h of fermentation. To our knowledge, this is the first report of the production of a bioproduct from cellobiose using E. coli.. |
| 22. |
Taizo Hanai, Metabolic Engineering for isopropanol Production, IBS, 2012.09, We investigated the production of the isopropanol in E. coli by heterologous expression of the Clostridial metabolic pathway which utilizes the metabolite acetyl-CoA. The synthetic pathway was constructed using combinations of genes from Clostridium acetobutylicum ATCC824, E. coli K-12 MG1655, Clostridium beijerinckii NRRL B593 and Thermoanaerobacter brockii HTD4. The acetone and isopropanol productions from glucose using these combinations of genes were compared. For acetone production, the best strain had the combination of genes thl (thiolase from C. acetobutylicum), atoAD (CoA transferase from E. coli) and adc (acetoacetate decarboxylase from C. acetobutylicum). Using this strain, the maximum production rate was 12.1 mM/h with 73.5 % maximum theoretical yield and the maximum concentration after exhausting the carbon source in a glucose fed batch shake flask experiment was 148.3 mM. For isopropanol production, the strain with the combination of genes thl, atoAD, adc and cbadh (secondary alcohol dehydrogenase from C. beijerinckii) achieved the highest titer. This strain produced 81.6 mM isopropanol in a glucose fed batch shake flask experiment. The maximum production rate of this strain was 6.9 mM/h with 43.5 % maximum theoretical yield. The strains presented in this study are the best acetone and isopropanol production strains yet reported, including Clostridia hosts containing the native production pathway. After the optimization of fermentation condition and using the gas stripping method, this strain produced 2,378 mM (143 g/L) of isopropanol after 240 h with a yield of 67.4% (mol/mol).
Efficient bio-production from lignocellulosic biomass is required for the purpose of developing an inexpensive, practical bio-refinery process. As one approach to address this problem, we genetically engineered E. coli to produce isopropanol directly from cellobiose via the cellobiose degradation by Beta-Glucosidase (BGL) on the cell surface. We introduced the synthetic pathway for isopropanol production and the BGL protein from Thermobifida fusca YX (Tfu0937) fused to the anchor protein Blc (Tfu-Blc) into E. coli and compared their isopropanol production in the presence of cellobiose. This strain consumed cellobiose and produced 69.0±11.6 mM isopropanol at 21 h of fermentation. To our knowledge, this is the first report of the production of a bioproduct from cellobiose using E. coli.
. |
| 23. |
Taizo Hanai, Synthetic pathway for isopropanol production in E. coli, iBioP, 2011.12, We investigated the production of the isopropanol in E. coli by heterologous expression of the Clostridial metabolic pathway which utilizes the metabolite acetyl-CoA. The pathway was constructed using combinations of genes from Clostridium acetobutylicum ATCC824, E. coli K-12 MG1655, Clostridium beijerinckii NRRL B593 and Thermoanaerobacter brockii HTD4. The acetone and isopropanol productions from glucose using these combinations of genes were compared. For acetone production, the best strain had the combination of genes thl (thiolase from C. acetobutylicum), atoAD (CoA transferase from E. coli) and adc (acetoacetate decarboxylase from C. acetobutylicum). Using this strain, the maximum production rate was 12.1 mM/h with 73.5 % maximum theoretical yield and the maximum concentration after exhausting the carbon source in a glucose fed batch shake flask experiment was 148.3 mM. For isopropanol production, the strain with the combination of genes thl, atoAD, adc and cbadh (secondary alcohol dehydrogenase from C. beijerinckii) achieved the highest titer. This strain produced 81.6 mM isopropanol in a glucose fed batch shake flask experiment. The maximum production rate of this strain was 6.9 mM/h with 43.5 % maximum theoretical yield. The strains presented in this study are the best acetone and isopropanol production strains yet reported, including Clostridia hosts containing the native production pathway. After the optimization of fermentation condition and using the gas stripping method, this strain produced 2,378 mM (143 g/L) of isopropanol after 240 h with a yield of 67.4% (mol/mol).. |
| 24. |
Metabolic engineering for C3, C4 bioalcohol production by E.coli. |
| 25. |
Metabolic Eingineering for isopropanol production by E. coli. |
| 26. |
Metabolic engineering of engineered E. coli for bioalocohol production. |
| 27. |
Study on culture condition of isopropanol production by engineered E. coli. |
