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Taizo Hanai Last modified date:2019.06.14

Associate Professor / Synthetic Biology
Department of Bioscience and Biotechnology
Faculty of Agriculture


Graduate School
Undergraduate School


E-Mail
Homepage
http://www.brs.kyushu-u.ac.jp/~taizo
Homepage of Associate Professor Hanai .
Phone
092-802-4730
Academic Degree
Doctor of Engineering
Country of degree conferring institution (Overseas)
No
Field of Specialization
Biochemical Engineering
Total Priod of education and research career in the foreign country
01years10months
Outline Activities
Research and Education

Improvement of Bio-fuel production by metabolic engineering
Creation of artificial genetic circuit by synthetic biology
Bioinformatics for bioengineering and medical application

Social activities
Editorial Board, Journal of Bioscience and Bioengineering (2009-2013)
Editorial Advisory Board, Bioprocess and Biosystems Engineering (2006-2013)
Member of Electrical Information Committee in The Society for Biotechnology, Japan (2006-2011)
Editor for Biomedia in The Society for Biotechnology, Japan (1998-2000)


Research
Research Interests
  • Application of AI to Biotechnology
    keyword : Deep Learning, Statistics, Biological Data
    2018.06~2019.06.
  • Construction of artifical gene network by synthetic biology
    keyword : Synthetic Biology, Artificial Genetic Circut, System Biology, Molecular Biology, Simulation, System Design, System Analysis
    2007.06Recently, small number of research groups started to study named "artificial genetic circuit" or "synthetic biology" in systems biology research area. This study aims to realize oscillation or complicated phenomenon of gene expression by analysis of interactions or control of genes or proteins and by combination of them artificially. In order to realize such kind complicated phenomenon, we must estimate dynamisms of large number of bio-molecules. For this purpose, not only gene manipulation, but also simulation and system analysis are needed. Now, we are tying to create biological systems to realize oscillator of gene expression..
  • Improvement of biofuel production by metabolic engineering
    keyword : Metabolic Engineering, Bioalcohol, Metabolic Flux Analysis, Simulation, Bioinformatics, Transcriptome, Proteome, Metabolome
    2006.08Recently, rapid increasing of oil price has made us pay attention to biofuel production. We have already succeeded to produce some types of alcohols by unnatural producer E.coli. It is well known that E.coli has a fast growth rate and one of most user-friendly organisms for gene manipulation. These merits can help improvement of the biofuel production. We use the simulation technology to estimate the flux of metabolite. Based on the simulation result, we can decide the enzyme to be modified or over expressed or deleted. Produced alcohols are poison for E.coli, so it is also important to create the stronger E.coli against alcohols. For this purpose, we are using the analysis result of the data from transcriptome, proteome and metabolome by bioinformatics techniques..
Current and Past Project
  • Recently, rapid increasing of oil price has made us pay attention to biofuel production. We have already succeeded to produce some types of alcohols by unnatural producer E.coli. It is well known that E.coli has a fast growth rate and one of most user-friendly organisms for gene manipulation. These merits can help improvement of the biofuel production. We use the simulation technology to estimate the flux of metabolite. Based on the simulation result, we can decide the enzyme to be modified or over expressed or deleted. Produced alcohols are poison for E.coli, so it is also important to create the stronger E.coli against alcohols. For this purpose, we are using the analysis result of the data from transcriptome, proteome and metabolome by bioinformatics techniques.
  • In order to produce bioalcohol from carbon dioxide and solar energy, engineered cynobacteria is created.
  • Recently, small number of research groups started to study named "artificial genetic circuit" or "synthetic biology" in systems biology research area. This study aims to realize oscillation or complicated phenomenon of gene expression by analysis of interactions or control of genes or proteins and by combination of them artificially. In order to realize such kind complicated phenomenon, we must estimate dynamisms of large number of bio-molecules. For this purpose, not only gene manipulation, but also simulation and system analysis are needed. Now, we are tying to create biological systems to realize oscillator of gene expression.
Academic Activities
Books
1. Taizo Hanai, Hiroyuki Honda, Takeshi Kobayashi, Soft Computing in Chemistry, Springer-Verlag, p.135-159, 2003.03.
Reports
1. Taizo Hanai, Hiroyuki Hamada, Masahiro Okamoto, Application of bioinformatics for DNA microarray data to Bioscience, 2006.05.
Papers
1. Yasutaka Hirokawa, Yuki Maki, Taizo Hanai, Improvement of 1,3-propanediol production using an engineered cyanobacterium, Synechococcus elongatus by optimization of the gene expression level of a synthetic metabolic pathway and production conditions, METABOLIC ENGINEERING, 39, 192-199, 2017.03.
2. Yuki Soma, Keigo Tsuruno, Masaru Wada, Atsushi Yokota, Taizo Hanai, Metabolic flux redirection from a central metabolic pathway toward a synthetic pathway using a
metabolic toggle switch, Metabolic Engineering, 10.1016/j.ymben.2014.02.008, 23, 175-184, 2014.02, Overexpression of genes in production pathways and permanent knockout of genes in competing pathways are often employed to improve production titer and yield in metabolic engineering. However, the deletion of a pathway responsible for growth and cell maintenance has not previously been employed, even if its competition with the production pathway is obvious. In order to optimize intracellular metabolism at each fermentation phase for bacterial growth and production, a
methodology employing conditional knockout is required. We constructed a metabolic toggle switch in E. coli as a novel conditional knockout approach and applied it to isopropanol production. The resulting redirection of excess carbon flux caused by interruption of the TCA cycle via switching gltA OFF improved isopropanol production titer and yield up to 3.7 and 3.1 times, respectively. This approach is a useful tool to redirect carbon flux responsible for bacterial growth and/or cell
maintenance toward a synthetic production pathway..
3. Tamami Kusakabe, Tsuneyuki Tatsuke, Keigo Tsuruno, Shota Atsumi, James C. Liao, Taizo Hanai, Engineering synthetic pathway in cyanobacteria for isopropanol production directly from carbon dioxide and light, Metabolic Engineering, 10.1016/j.ymben.2013.09.007, 20, 101-108, 2013.09, Production of alternate fuels or chemicals directly from solar energy and carbon dioxide using engineered cyanobacteria is an attractive method to reduce petroleum dependency and minimize carbon emission. Here, a synthetic pathway using thl (acetyl-CoA acetyltransferase) and adc (acetoacetate decarboxylase) from Clostridium acetobutylicum ATCC824, and atoAD (acetoacetyl-CoA transferase) from Escherichia coli K-12 MG1655, and adh (secondary alcohol dehydrogenase) from Clostridium beijerinckii NRRL B593 were integrated into a genome of Synechococcus elongatus strain PCC7942 to produce isopropanol. Under optimized production conditions, the engineered cyanobacteria produced 26.5 mg/L of isopropanol after 9
days. While nitrogen starving conditions improved isopropanol production 7.2-fold, dark conditions lead to a 27-fold improvement over light conditions..
4. Shota Atsumi, Anthony F. Cann, Michael R. Connor, Claire R. Shen, Kevin M. Smith, Mark P. Brynildsen, Katherine J.Y. Chou, Taizo Hanai, James C. Liao, Metabolic engineering of Escherichia coli for 1-butanol production, Metabolic Engineering, 10(6), 305-311, 2008.11.
5. Shota Atsumi, Taizo Hanai, James C. Liao, Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels, Nature, 451, 7174, 86-89, 2008.01, 再生可能資源であるバイオマスから、代替燃料イソブタノールを効率的に生産する方法を開発することは重要である。本研究では、組換えが容易な大腸菌に、Ehrlich経路(合成代謝経路)を外部から導入し、本来生産されないイソブタノールを生産することに成功した。また、この生産大腸菌のもつ目的生産物との競合代謝経路遺伝子を破壊、または必要な反応酵素遺伝子を増強し、20g/Lまでの生産性の向上を達成した。.
6. Taizo Hanai, Shota Atsumi, James C. Liao, Engineered synthetic pathway for isopropanol production in Escherichia coli, Applied and Environmental Microbiology, 73, 24, 7814-7818 , 2007.12.
7. Koujiro Nishida, Shinji Mine, Tohru Utsunomiya, Hiroshi, Inoue, Masahiro Okamoto, Harushi Udagawa, Taizo Hanai, Masaki Mori, Global analysis of altered gene expressions during the process esophageal squamous cell carcinogenesis in the rat: a study combined with a laser microdissection and cDNA microarray, Cancer Research, 65, 2, 401-409, 65, 2, 401-409, 2005.02.
8. Shuta Tomida, Taizo Hanai, Hiroyuki Honda and Takeshi Kobayashi, Gene expression analysis using fuzzy ART model, Bioinformatics, 18, 8, 1073-1083, 2002.08.
Presentations
1. Yuki Soma, Taizo Hanai, Synthetic quorum sensing as a tunable cell density sensor-regulator for dynamic metabolic engineering, Metabolic Engineering XI, 2016.06.
Awards
  • Direct isopropanol production from cellobiose by engineered Escherichia coli using a synthetic pathway and a cell surface display system
Social
Professional and Outreach Activities
NO.