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Michiya Matsuyama Last modified date:2019.06.15

Professor / Animal and Marine Bioresource Sciences
Department of Bioresource Sciences
Faculty of Agriculture


Graduate School
Undergraduate School
Other Organization
Administration Post
Vice President


Homepage
http://www.agr.kyushu-u.ac.jp/asweb/sui1/lmb.html
Our laboratory focuses on science for basic mechanism of fish gametogenesis and effective production of fish. .
Academic Degree
Doctor of Agriculture
Country of degree conferring institution (Overseas)
No
Field of Specialization
Fisheries Science
Outline Activities
The purpose of our laboratory is an elucidation of reproductive physiology of marine organism, especially fishes, and its application for fishery. The present main research themes are as follows.
1. Endocrinological mechanism of fish gametogenesis
2. Development of techniques in fish seed production
3. Population ecology of fish
As a student, seven master courses and five graduation-thesis students are on the register now, and they are working in one of the above-mentioned research themes, respectively. It always strives for the newest analysis technology and ingestion of theory through a seminar (1 time per week), and a progress report (1 time per month). While letting joint research with other laboratories pass and calculating the increase in efficiency of research, the responsibility as students' researcher and consciousness are urged. Through joint research with other laboratories calculating the increase in efficiency of research, and the responsibility as researcher and consciousness are urged. Furthermore, students are made to strive for acquisition of a cosmopolitan outlook by making them participate to international research meetings. As social activity, we mainly promote the industrial, administrative and academic sectors research by cooperation with some local self-governing bodies and private corporations.
Research
Research Interests
  • Endocrine mechanism of reproduction in Fish, Sex determination, differentiation and sex change in fish, Fisheries biology of small pelagic fish, Development of seed production technique in fish, Genome editing and breeding
    keyword : gametogenesis, sex differentiarion and change, small pelegic fish, seed production, genome editing
    2000.03In parallel to basic research of molecular and cellular mechanism on gametogenesis, sex change, sex differentiation, and egg quality of fish, research on the biological characteristic of wild fish for resource management and on development of seed production technique of useful fishes are performed..
Academic Activities
Books
1. Michiya Matsuyama, Egg Production, 109 pp. In “HANDBOOK OF APPLIED FISHERIES REPRODUCTIVE BIOLOGY FOR STOCK ASSESSMENT AND MANAGEMENT” (R. Domínguez-Petit, H. Murua, F. Saborido-Rey and E. Trippel, ed), 2014.12.
2. Michiya Matsuyama, Sethu Selvaraj, Mitsuo Nyuji, Hirofumi Ohga, Involvement of Brain-Pituitary-Gonadal Axis on Regulation of the Reproductive Cycle in Female Chub mackerel. In “Sexual Plasticity and Gametogenesis in Fishes” (Senthilkumaran B, ed), Nova Science Publishers, 251-274, 2013.08.
3. M. Matsuyama, Follicular Steroidogenesis in Fish, Science Publishers (USA), In “Fish Reproduction” (Rocha MJ, Arukwe A, Kapoor BG, eds.), 171-199.
, 2007.12.
4. Reproductive Physiology in "Biology, Stock Enhancement and Aquaculture of Buri (Japanese yellowtail, Seriola quinqueradiata)".
5. Sex steroid hormones.
6. Gametogenesis in Fish -Basic and Applied Science in Fisheries-.
Reports
1. Application of Reproductive Physiology to the Advanced Study of Stock Productivity of Small Pelagic Fishes.
Papers
1. Sakaguchi K, Yoneda M, Sakai N, Nakashima K, Kitano H, Matsuyama M, Comprehensive Experimental System for a Promising Model Organism Candidate for Marine Teleosts, Scientific Reports, org/10.1038/ s41598-019-41468-8, 9, Article number 4948, 2019.03, A comprehensive experimental system for Japanese anchovy, a promising candidate model organism for marine teleosts, was established. Through the design of a rearing/spawning facility that controls the photoperiod and water temperature, one-cell eggs were continuously obtained shortly after spawning throughout the rearing period. The stages of eggs are indispensable for microinjection experiments, and we developed an efficient and robust microinjection system for the Japanese anchovy. Embryos injected with GFP mRNA showed strong whole-body GFP fluorescence and the survival rates of injected- and non-injected embryos were not significantly different, 87.5% (28 in 32 embryos) and 90.0% (45 in 50 embryos), respectively. We verified that the Tol2 transposon system, which mediates gene transfer in vertebrates, worked efficiently in the Japanese anchovy using the transient transgenesis protocol, with GFP or DsRed as the reporter gene. Finally, we confirmed that genome-editing technologies, namely Transcription Activator-Like Effector Nucleases (TALEN) and Clustered Regulatory Interspaced Short Palindromic Repeats (CRISPR)/Cas9, were applicable to the Japanese anchovy. In practice, specific gene-disrupted fishes were generated in the F1 generation. These results demonstrated the establishment of a basic, yet comprehensive, experimental system, which could be employed to undertake experiments using the Japanese anchovy as a model organism for marine teleost fish..
2. Ohga H, Selvaraj S, Matsuyama M, The Roles of Kisspeptin System in the Reproductive Physiology of Fish with Special Reference to Chub Mackerel Studies as Main Axis, Frontiers in Endocrinology, 10.3389/fendo.2018.00147, 9, 1-15, 2018.10, Kisspeptin, a novel neuropeptide product of the Kiss1 gene, activates the G protein-coupled membrane receptor G protein-coupled receptor 54 (now termed Kiss1r). Over the last 15 years, the importance of the kisspeptin system has been the subject of much debate in the mammalian research field. At the heart of the debate is whether kisspeptin is an absolute upstream regulator of gonadotropin-releasing hormone secretion, as it has been proposed to be the master molecule in reproductive events and plays a special role not only during puberty but also in adulthood. The teleostean kisspeptin system was first documented in 2004. Although there have been a number of kisspeptin studies in various fish species, the role of kisspeptin in reproduction remains a subject of controversy and has not been widely recognized. There is an extensive literature on the physiological and endocrinological bases of gametogenesis in fish, largely derived from studying small, model fish species, and reports on non-model species are limited. The reason for this discrepancy is the technical difficulty inherent in developing rigorous experimental systems in many farmed fish species. We have already established methods for the full life-cycle breeding of a commercially important marine fish, the chub mackerel (cm), and are interested in understanding the reproductive function of kisspeptins from various perspectives. Based on a series of experiments clarifying the role of the brain-pituitary-gonad axis in modulating reproduction in cm, we theorize that the kisspeptin system plays an important role in the reproduction of this scombroid species. In this review article, we provide an overview of kisspeptin studies in cm, which substantially aids in elucidating the role of kisspeptins in fish reproduction..
3. Hirofumi Ohga, Hayato Adachi, Hajime Kitano, Akihiko Yamaguchi, Michiya Matsuyama, Kiss1 hexadecapeptide directly regulates gonadotropin-releasing hormone 1 in the scombroid fish, chub mackerel, Biology of Reproduction, 10.1095/biolreprod.116.142083, 96, 2, 376-388, 2017.02, Here we report that the Kiss1 hexadecapeptide (Kiss1-16) directly regulates the functional form of gonadotropin-releasing hormone (GnRH) in the preoptic area (POA) of a scombroid fish model. In this study, we analyzed the localization of two kisspeptin (kiss1 and kiss2) neurons and two kisspeptin receptors (kissr1 and kissr2) in the brain of adult chub mackerel using in situ hybridization to determine whether the kisspeptin receptors co-localize with GnRH1 neurons. The kiss1-and kiss2-expressing neurons were mainly localized in the nucleus recessus lateralis (NRL) and the nucleus of the posterior recess (NRP) in the hypothalamus. Kissr1 was present in the anterior POA and the habenular nucleus. Kissr2 was widely distributed, including in the POA, lateral tuberal nucleus, NRL, and NRP. Notably, GnRH1 was expressed in neurons in the POA, and these neurons co-expressed kissr1. In contrast, kissr2 was expressed abundantly in the vicinity of GnRH1 neurons, but their co-expression did not seem to occur. We also characterized the endogenous mature form of the Kiss1 peptide. An in vitro reporter gene assay clearly showed that Kiss1-16 (HQDMSSYNFNSFGLRY-NH2) was more potent at receptor activation than Kiss1 pentadecapeptide (Kiss1-15), which is the form of Kiss1 found in other fish species. This study strongly suggests that kisspeptin signaling, especially Kiss1 signaling, is important for regulating reproduction in scombroid fish..
Presentations
1. Immunocytochemical identification of two different GtH cells and changes of GtH activities during reproductive cycles in the pituitary of chub mackerel, Scomber japonicus.
2. Brain type aromatase expression in gonad and brain and its role in germ cell proliferation in Japanese pufferfish, Takifugu rubripes..
Membership in Academic Society
  • The Japanese Society of Fisheries Science
  • Japanese Society for Aquaculture Research
  • Japanese Society of Fisheries Oceanography
  • The Japan Society for Comparative Endocrinology
Awards
  • Studies on the Reproductive Physiology of Fish for Aquaculture and Management of Fishery Resources