Kyushu University Academic Staff Educational and Research Activities Database
Researcher information (To researchers) Need Help? How to update
Toshiki Tsurimoto Last modified date:2016.09.27

Professor / Integrative Biology
Department of Biology
Faculty of Sciences


Graduate School
Undergraduate School
Other Organization


Homepage
http://seibutsu.biology.kyushu-u.ac.jp/~chromosome/
The Homepage of the Research Team for Chromosomal DNA Replication in Department of Biology, Faculty of Sciences, Kyushu University .
Academic Degree
PhD in Science
Field of Specialization
Molecular Biology, Biochemistry
Outline Activities
I have been studying molecular mechanism of DNA replication in human cells focusing on its functional links with cellular factors involved in maintenance of the genome integrity during cell proliferation.
Through these researches, I offered basic knowledge for molecular biology and advanced technology for studies on protein functions to undergraduate- and graduate- students.
Since my research subjects also have tight relation with mechanism of cancer development, it can be expected to provide a new idea to develop anti-cancer drug. Therefore, I have actively advanced collaborative researches with cancer researchers.
Research
Research Interests
  • Molecular mechanism of eukaryotic chromosomal DNA replication and its regulation
    keyword : replication/cell cycle/ DNA polymearse/ clamp/ clamp loader/ AAA+ ATPase
    1991.07~2020.03I have been studying molecular mechanism of DNA replication in human cells focusing on its functional links with cellular factors involved in maintenance of the genome integrity during cell proliferation..
Academic Activities
Reports
1. Tsurimoto T., PCNA interacting proteins., Proliferating Cell Nuclear Antigen (PCNA). ed. Hoyun Lee, Research Signpost, Kerala, India, 2006.09.
2. Tsurimoto, T., PCNA and RFC proteins in Genomic Stability., DNA Replication and Human Disease ed. DePamphilis, M. L, Cold Spring Harbor Press NY, 2006.09.
Papers
1. Shiomi, Y., Masutani, C., Hanaoka, F., Kimura H. and Tsurimoto, T. , A second PCNA loader complex, Ctf18-RFC, stimulates DNA polymerase η activity. , J. Biol. Chem., 282, 20906-20914 , 2007.07.
2. Ohta, S., Shiomi, Y., Sugimoto, K., Obuse, C., Tsurimoto, T., A proteomics approach to identify PCNA binding proteins in human cell lysates: identification of the human CHL12/RFCs2-5 complex as a novel PCNA binding protein., J. Biol. Chem., 277, 40362-40367, 2002.01.
3. Shiomi, Y., Usukura, J., Masamura, Y., Takeyasu, K., Nakayama, Y., Obuse, C., Yoshikawa, H. Tsurimoto, T., ATP-dependent structural change of the eukaryotic clamp loader protein, RFC., Proc. Natl. Acad. Sci. USA, 97, 14127-14132, 2000.01.
Presentations
1. Toshiki Tsurimoto, Fujisawa R, Eiji Ohashi, Seiji Tanaka, Hiroyuki Araki, Sun Q, Kaye K, Novel mechanisms of PCNA loader complexes to specify their functions. , The 9th 3R Symposium, 2014.11.20.
2. Hiroko Okimoto, Seiji Tanaka, Hiroyuki Araki, Eiji Ohashi, Toshiki Tsurimoto, Roles of loader proteins for coupling between replication and chromosome maintenance, 新学術領域研究「ゲノム普遍的制御」主催、International Conference"Coupling of replication, repair and transcription, and their common mechanism of chromatin remodeling"公開シンポジウム, 2014.02.05.
3. Hiroko Okimoto, Seiji Tanaka, Hiroyuki Araki, Eiji Ohashi, Toshiki Tsurimoto, Functions of the C-terminal of the chromosome cohesion PCNA loader, Ctf18-RFC, Cold Spring Harbor Lab. Eukaryotic DNA Replication & Genome Maintenanceワークショップ, 2013.09.11.
4. Function of the holocomplex between DNA polymerase e and PCNA loader Ctf18-RFC.
5. Molecular crosstalk between DNA synthesis and chromosome cohesion apparatuses.
6. 釣本 敏樹, Okimoto H, Tanaka S, Araki H, 大橋 英治, Two functional structures of the chromosome cohesion PCNA loader, Ctf18-RFC , 第8回3Rシンポジウム, 2012.11.25.
7. The second PCNA loader Ctf18-RFC interacts with DNA polymerase ε in human cells..
8. Analysis of intrinsic DNA binding activity of 9-1-1 complex.
9. Roles of the 9-1-1/TopBP1 interaction for DNA damage responses in human cells..
10. Studies on molecular interactions between clamp loader complexes and MCM subcomplexes.
11. Studies on functional domains of human DNA polymerase epsilon catalytic subunit p261.
12. Studies on molecular interactions between clamp loader complexes and MCM subcomplexes.
13. Stimulation DNA polymerase activity with ubiquitianted PCNA.
14. Studies on molecular interactions between clamp loader complexes and DNA replication proteins.
15. Analyses on interaction between loader complexes and replication proteins in human cells.
16. STUDIES ON MOLECULAR INTERACTIONS BETWEEN LOADER COMPLEXES AND REPLICATION FORK COMPONENTS.
17. Studies on molecular interactions of clamp loader complexes with DNA polymerase e .
18. Studies on molecular interactions of clamp loader complexes with DNA polymerase e and RPA.
19. Studies on molecular interactions between clamp loader complexes and DNA replication proteins.
20. Stimulation DNA polymerase activity with ubiquitianted PCNA.
Educational
Other Educational Activities
  • 2009.03.
  • 2008.08.
  • 2008.03.
  • 2007.03.
  • 2007.07.