Kyushu University Academic Staff Educational and Research Activities Database
List of Papers
Keiji Takasu Last modified date:2018.12.24

Professor / Agronomy and Environmental Sciences / Department of Bioresource Sciences / Faculty of Agriculture

1. Keiji Takasua, Jemimah N. Ndabaruab, Hieu Thi Pham, Shun-ichiro Takanoa, Pupal-adult parasitism of the coconut hispine beetle by the koinobiont pupal parasitoid Tetrastichus brontispae, Biological Control, 2018.07.
2. Shunichiro Takano, Keiji Takasu, Matias Tavares, Marcal Gusmao, Acacio Cardoso Amaral, Differences in invasiveness between two cryptic species of the coconut beetle Brontispa longissima in Timor-Leste, Biological Invasions, 10.1007/s10530-017-1394-4, 19, 6, 1839-1851, 2017.06, Brontispa longissima (Coleoptera: Chrysomelidae) is a serious pest of coconut palm, and the species contains two cryptic species: the “Asian clade” is distributed over a wide area, including Asia and the Pacific region, whereas the “Pacific clade” is distributed in a limited area. Recent invasions and outbreaks have only been reported for the Asian clade, suggesting that invasive ability may differ between the clades. To reveal differences in invasiveness, we investigated the damage potential on coconut palm and range expansion of the two clades in Timor-Leste, where both clades are present. Distribution of the clades and of severely damaged trees indicated that range expansion and outbreaks have occurred for the Asian clade but not for the Pacific clade. The Asian clade attacked trees taller than 10 m, whereas the Pacific clade seldom attacked these trees. The preference for the taller trees, which are more abundant, can facilitate range expansion and outbreaks of the Asian clade. The beetle has spread through areas where coconut palms are continuously available, but have not expanded their distribution where coconut palms are separated by large gaps. This indicates that areas free of coconut palm could serve as buffer zones to prevent spread of this beetle..
3. Keisuke Hoshino, Taro Adati, Dawn M. Olson, Keiji Takasu, Seasonal occurrence and interspecific interactions of egg parasitoids of megacopta cribraria (heteroptera
Plataspidae) in Japan, Environmental Entomology, 10.1093/ee/nvx060, 46, 3, 487-493, 2017.06, We conducted a field study to determine seasonal egg parasitism rates of the kudzu bug Megacopta cribraria (F.) on the kudzu plant, Pueraria Montana (Lour.) Merr. var. lobata (Willd.) Maesen et Almeida ex Sanjappa and Pradeep, in Tokyo, Japan, during the period from May 2014 to September 2014. The eggs of M. cribraria per 1 m2 of kudzu at four locations in Tokyo were collected weekly and parasitism rates were assessed. Eggs of M. cribraria were laid on the kudzu plant from May to September. Megacopta cribraria eggs were parasitized by two parasitoid species, Paratelenomus saccharalis (Dodd) and Ooencyrtus nezarae Ishii. Paratelenomus saccharalis first appeared in May, and its parasitism rates peaked in July and September. Ooencyrtus nezarae first appeared in June and its parasitism rates peaked in July. Except for one location which could not be statistically analyzed because of the small sample size, occurrence of parasitism by P. saccharalis and O. nezarae in M. cribraria egg masses was independent at one location and positively associated at two locations, suggesting that the use of host egg masses by P. saccharalis and O. nezarae is not mutually exclusive. Parasitism rates by P. saccharalis and O. nezarae were significantly lower for egg masses parasitized by both species than for those parasitized by a single species. The proportion of males among O. nezarae progeny was significantly higher for egg masses parasitized by O. nezarae together with P. saccharalis than for those parasitized by O. nezarae alone. These results suggest that parasitism of host egg masses by the two species is influenced by their interspecific interactions..
4. Shunichiro Takano, Midori Tuda, Keiji Takasu, Naruto Furuya, Yuya Imamura, Sangwan Kim, Kosuke Tashiro, Kazuhiro Iiyama, Matias Tavares, Acacio Cardoso Amaral, Unique clade of alphaproteobacterial endosymbionts induces complete cytoplasmic incompatibility in the coconut beetle, Proceedings of the National Academy of Sciences of the United States of America, 10.1073/pnas.1618094114, 114, 23, 6110-6115, 2017.06, Maternally inherited bacterial endosymbionts in arthropods manipulate host reproduction to increase the fitness of infected females. Cytoplasmic incompatibility (CI) is one such manipulation, in which uninfected females produce few or no offspring when they mate with infected males. To date, two bacterial endosymbionts, Wolbachia and Cardinium, have been reported as CI inducers. Only Wolbachia induces complete CI, which causes 100% offspring mortality in incompatible crosses. Here we report a third CI inducer that belongs to a unique clade of Alphaproteobacteria detected within the coconut beetle, Brontispa longissima. This beetle comprises two cryptic species, the Asian clade and the Pacific clade, which show incompatibility in hybrid crosses. Different bacterial endosymbionts, a unique clade of Alphaproteobacteria in the Pacific clade and Wolbachia in the Asian clade, induced bidirectional CI between hosts. The former induced complete CI (100% mortality), whereas the latter induced partial CI (70% mortality). Illumina MiSeq sequencing and denaturing gradient gel electrophoresis patterns showed that the predominant bacterium detected in the Pacific clade of B. longissima was this unique clade of Alphaproteobacteria alone, indicating that this endosymbiont was responsible for the complete CI. Sex distortion did not occur in any of the tested crosses. The 1,160 bp of 16S rRNA gene sequence obtained for this endosymbiont had only 89.3% identity with that of Wolbachia, indicating that it can be recognized as a distinct species. We discuss the potential use of this bacterium as a biological control agent..
5. Jean Philippe Parent, Keiji Takasu, Jacques Brodeur, Guy Boivin, Time perception-based decision making in a parasitoid wasp, Behavioral Ecology, 10.1093/beheco/arw171, 28, 3, 640-644, 2017.05, The capacity of animals to measure time and adjust their behaviors accordingly has been a topic of interest in vertebrates, but little evidence is currently available for insects. This capacity has yet to be properly investigated in parasitoid wasps, even though they are frequently used to test ecological models. Here, using associative learning between odors and time intervals, we show that the parasitoid wasp Microplitis croceipes (Hymenoptera: Braconidae) has the capacity to measure time. When released in a wind tunnel, females flew toward an odor associated with the time interval they had just experienced. We also found that reducing energy expenditure by restraining parasitoid wasp movement during the training interval prevented time perception. This serves as experimental evidence of time perception in a parasitoid wasp, provides both a rare example of learning associated to a time interval in an insect and a mechanism by which these animals could optimize their behaviors, as well as suggesting a role for energy expenditure in its time perception mechanism..
6. William A. Overholt, Purnama Hidayat, Bruno Le Ru, Keiji Takasu, John A. Goolsby, Alex Racelis, A. Millie Burrell, Divina Amalin, Winnifred Agum, Mohamed Njaku, Beatrice Pallangyo, Patricia E. Klein, James P. Cuda, Potential Biological Control Agents for Management of Cogongrass (Cyperales
Poaceae) in the Southeastern USA, Florida Entomologist, 10.1653/024.099.0425, 99, 4, 734-739, 2016.12, Cogongrass, Imperata cylindrica (L.) Palisot de Beauvois (Cyperales: Poaceae), is a serious invasive weed in the southeastern USA. Surveys for potential biological control agents of cogongrass were conducted in Asia and East Africa from 2013 to 2016. Several insect herbivores were found that may have restricted host ranges based on field collection data and life histories. Stemborers in the genus Acrapex (Lepidoptera: Noctuidae) were collected from cogongrass in Tanzania, Uganda, and Japan. In the Philippines, larvae of Emmalocera sp. (Lepidoptera: Pyralidae) and Chilo sp. (Lepidoptera: Crambidae) were found boring in cogongrass. Cecidomyiid midges were found in both Japan and Indonesia. A Japanese midge identified as a Contarinia sp. (Diptera: Cecidomyiidae) caused deformation of the stem, whereas the Indonesian midge Orseolia javanica Kieffer & van Leeuwen-Reijinvaan (Diptera: Cecidomyiidae) induced the formation of a basal stem gall. Previous research suggested that the host range of O. javanica was restricted to cogongrass..
7. Shunichiro Takano, Keiji Takasu, Ability of Megacopta cribraria (Hemiptera
Plataspidae) to Cling to Different Surfaces against Extreme Wind, Journal of Insect Behavior, 10.1007/s10905-016-9557-1, 29, 3, 256-261, 2016.05, The kudzu bug, Megacopta cribraria (Fabricius), is native to Asia but recently invaded the US and is expanding its distribution rapidly. To assess the probability of this bug traveling by attaching to the exteriors of fast-moving vehicles, we investigated the ability of M. cribraria adults to cling to stages with different surfaces (cloth, metal, or glass) against extreme airflows in a transparent acrylic tube connected to a vacuum cleaner. On the stages with cloth and metal, insects remained on the stages at 100 km/h wind speed. Estimated wind velocities required to blow 50 % of insects from the stages within 1 min were more than 100 km/h (cloth); 60 km/h (metal); and 40 km/h (glass) for males and 100 km/h (cloth); 50 km/h (metal); and 30 km/h (glass) for females. Together with frequent observations of attached M. cribraria on vehicle exteriors in the field, our results indicate a fairly high probability of range expansion of this species by attachment to vehicles..
8. K. Takasu, Y. Yoshiyasu, A. M. Burrell, P.E. Klein, A. Racelis, J. A. Goosby, W. A. Overholt, Acrapex azumai Sugi (Lepidoptera: Noctuidae) as a possible biological control agent of the invasive weed Imperata cylindrica (L.) Beauv. (Poaceae) in the United States, Lepidoptera Science, 65, 1, 30-35, 2014.04.
9. J. K. Makatiani, H. K. Le, D. M. Olson, F. L. Wäckers, Keiji Takasu, An acquired distaste
Sugar discrimination by the larval parasitoid Microplitis croceipes (Hymenoptera: Braconidae) is affected by prior sugar exposure, Journal of Experimental Biology, 10.1242/jeb.091843, 217, 10, 1692-1700, 2014.01, We examined gustatory responses of the larval parasitoid Microplitis croceipes to determine whether the adults discriminate among common sugars, including fructose, glucose, maltose and sucrose, found in plants. When given single sugar solutions of sucrose, glucose, fructose and maltose at concentrations of 0.008-2.0 mol l-1, the estimated concentrations at which 50% of wasps initiated feeding ranged between 0.054 and 0.085 mol l-1 for sucrose, glucose and fructose, which was significantly lower than for maltose. Wasps showed a strong decrease in feeding time for maltose or fructose following a brief exposure to other sugars, suggesting that wasps can distinguish maltose and fructose from the other sugars tested. The higher acceptance threshold and short feeding time in the case of maltose appears adaptive in light of the relatively poor nutritional quality of the sugar in the longevity trial. The pronounced feeding inhibition seen for fructose following exposure to other sugars is not linked with lower nutritional performance. This feeding inhibition was even seen in wasps that had fed on glucose at the lowest acceptance threshold (0.031 mol l-1) and persisted for 24 h. This study is the first to show feeding inhibition of otherwise phagostimulant sugars such as maltose and fructose after gustatory stimulation on other sugars..
10. J. K. Makatiani, A. Y. Bruce, F. Schulthess, Keiji Takasu, Reproductive strategies of the larval parasitoid Microplitis croceipes
, Entomologia Experimentalis et Applicata, 10.1111/eea.12099, 148 , 3, 223-233, 2013.09.
11. Shun-ichiro Takano, Atsushi Mochizuki, Keiji Takasu, Kazuhiko Konishi, J. C. Alouw, D. S. Pandin, Satoshi Nakamura, Rapid discrimination of two cryptic species within Brontispa
longissima (Gestro) (Coleoptera: Chrysomelidae) by PCR–RFLP, Journal of Pest Science, 10.1007/s10340-012-0474-6, 86, 2, 151-155, 2013.06.
12. Ruberson, John R., Keiji Takasu, Buntin, G. David, From Asian curiosity to eruptive American pest: Megacopta cribraria (Hemiptera: Plataspidae) and prospects for its biological control, Applied Entomology and Zoology, 10.1007/s13355-012-0146-2, 48, 1, 3-13, 2013.02.
13. Takano, S., Takasu, K., Ichiki, R. T., Nakamura, S, Cold tolerance of the coconut hispine beetle Brontispa longissima (Coleoptera: Chrysomelidae) in Japan, Applied Entomology and Zoology, 10.1007/s13355-012-0105-y, 47, 3, 173-180, 2012.08.
14. H.T. Nguyen, T.T. Oo, R.T. Ichiki, S. Takano, M. Murata, K. Takasu, K. Konishi, S. Tunkumthong, N. Chomphookhiaw & S. Nakamura, Parasitisation of Tetrastichus brontispae (Hymenoptera: Eulophidae), a biological control agent of the coconut hispine beetle Brontispa
longissima (Coleoptera: Chrysomelidae), Biocontrol Science and Technology,, 22, 8, 955-968, 2012.07.
15. Shun-ichiro TAKANO, Keiji TAKASU, Tsutomu FUSHIMI, Ryoko T. ICHIKI and Satoshi NAKAMURA, Life history traits and damage potential of an invasive pest Brontispa longissima (Coleoptera: Chrysomelidae) on
Satakentia liukiuensisens, Entomological Science, 10.1111/j.1479-8298.2011.00506.x, 15, 238–245, 2012.04.
16. Takano, S., Takasu, K., Ichiki, RT., Fushimi, T., and Nakamura, S, Induction of host-plant preference in Brontispa longissima (Gestro) (Coleoptera: Chrysomelidae), Journal of Applied Entomology, 135, 1-7, 2011.04.
17. Takano, S., Mochizuki, A., Konishi, K., Takasu, K., and Nakamura, S. , Two cryptic species in Brontispa longissima (Colenoptera: Chrysomelidae): evidence from mitochondrial DNA analysis and crosses between the two nominal species., Annals of Entomological Society of America, 104, 121-131, 2011.03.
18. Takano, S., Sugeno, S., Mochizuki, A., Nakamura, S. and Takasu, K., Occurrence and distribution of the coconut hispine beetle Brontispa longissima in the Southwest islands of Japan, Proceedings of 7th International AFAS Joint Symposium between Korea and Japan, 149-154, 2010.11.
19. Ai Yamashita and Keiji Takasu, Suitability of potential host plants in Japan for immature development of the coconut hispine beetle, Brontispa longissima Gestro (Coleoptera: Chrysomelidae) , JARQ, 44, 2, 2010.04.
20. Andrei Alyokhin , Jacqueline Makatiani and Keiji Takasu, Insecticide Odour Interference with Food-Searching Behaviour of Microplitis croceipes (Hymenoptera: Braconidae) in a Laboratory Arena, Biocontrol Science and Technology, 2010.04.
21. Kyawt San Dar Aung, Keiji Takasu, Takatoshi Ueno and Masami Takagi, Effect of temperature on egg maturation and longevity of the egg parasitoid Ooencyrtus nezarae (Ishii) (Hymenoptera: Encyrtidae), J. Fac. Agr. Kyushu Univ., 55, 1, 87-89, 2010.04.
22. Takasu, K., Takano, S., Konishi, K., and Nakamura, S., An invasive pest Brontispa longissima (Gestro) (Coleoptera: Chrysomelidae) attacks an endemic palm in the Yaeyama Islands, Japan, Applied Entomology and Zoology, 2010.01.
23. Ai Yamashita, Amporn Winotai, Keiji Takasu, Use of mature leaves of coconut palm and the cattail for laboratory rearing of the coconut leaf beetle Brontispa longissima, Journal of Agricultural Science and Technology at CNU, 2009.06.
24. Huong Lan Bui, Keiji Takasu, Learning of narcotic odors by a parasitoid , Journal of Agricultural Science and Technology at CNU, 2009.06.
25. Ah Nge Htwe, Masami Takagi and Keiji Takasu, Development of Myanmar strain of Cotesia vestalis (Hymenoptera: Braconidae) on its host plutella xylostella (Lepidoptera: Plutellae) at different temperatures, J. Fac. Agr. Kyushu Univ., 53 (2) 441-446, 2009.03.
26. Ah Nge Htwe, Keiji Takasu and Masami Takagi, Laboratory rearing of the diamondback moth Plutella xylostella (L.) (Lepidoptera: Plutellidae) with artificial diet , J. Fac. Agr. Kyushu Univ., 54 (1) 147-151, 2009.03.
27. Ai Yamashita, Keiji Takasu, Suitability of potential host plants in Japan for immature development of the coconut hispine beetle, Brontispa longissima Gestro (Coleoptera: Chrysomelidae) , Bulletin of the Institute of Tropical Agriculture Kyushu University, vol. 32, 2009.03.
28. Keiji Takasu and Satoshi Nakamura, Life history of the tick parasitoid Ixodiphagus hookeri (Hymenoptera: Encyrtidae) in Kenya, Biological Control, 2008.05.
29. Keiji Takasu, Kazuo Ogata, Ah Nge Htwe, Giang Thi Thu Ho and Masami Takagi, Winter crop problems associated with the introduction of hybrid rice: protection of crucifer crops against DBM, Proceedings of the JSPS International Seminar 2007, Hybrid rice and agroecosystem, 205-209, 2007.11.
30. Konishi K, Nakamura S, and Takasu K , Invasion of the coconut hispine beetle, Brontispa longissima: Current situation and control measures in Asia., Proceedings of International Symposium for Invasive Alien Species in Monsoon Asia: , pp. 1-7., 2007.10.
31. Keiji Takasu and Le K. H., The larval parasitoid Microplitis croceipes oviposits in conspecific adults., Naturwissenschaften 94:200-206., Naturwissenschaften 94:200-206., 2007.03.
32. Keiji Takasu, Rains R. C. and Lewis, W. J., Comparison of detection ability of learned odors between males and females in the larval parasitoid Microplitis croceipes (Hymenoptera: Braconidae)., Entomologia experimentalis et applicata, Entomologia. Exp. Appli. 122: 247-251., 2007.03.
33. D.M. Olson, G. C. Rains, T. Meiners, K. Takasu, M. Tertuliano, J. H. Tumlinson, F. L. Wackers, and W. J. Lewis, Parasitic wasps learn and report diverse chemicals with unique conditionable beehaviors, Chemical Senses, 10.1093/chemse/28.6.545, 28, 6, 545-549, 2003.08.
34. Keiji Takasu, Helicoverpa armigera as an alternative host of the larval parasitoid Microplitis croceipes (Hymenoptera: Braconidae), Applied Entomology and Zoology, 10.1303/aez.2005.679, 40, 4, 679-686, 2005.11.
35. Keiji Takasu, Importance of multidisciplinary research for insect pest management., Inst. Trop. Agric. Kyushu Univ., 28:43-49., 2005.12.
36. Olson, D. M., Lewis, W. J., and Keiji Takasu, Interactive-web of factors governing effective natural enemy foraging behavior. Overview of food resources as a critical component., International Symposium on Biological Control of Arthropods., pp389-397..