Kyushu University Academic Staff Educational and Research Activities Database
List of Papers
Kunio Ishikawa Last modified date:2018.06.22

Professor / Oral Rehabilitation / Department of Dental Science / Faculty of Dental Science


Papers
1. Yukie Shibata, Yoshihisa Yamashita, Kanji Tsuru, Kazuhiko Ishihara, Kyoko Fukazawa, Kunio Ishikawa, Preventive effects of a phospholipid polymer coating on PMMA on biofilm formation by oral streptococci, Applied Surface Science, 10.1016/j.apsusc.2016.08.108, 390, 602-607, 2016.12, The regulation of biofilm formation on dental materials such as denture bases is key to oral health. Recently, a biocompatible phospholipid polymer, poly(2-methacryloyloxyethyl phosphorylcholine-co-n-butyl methacrylate) (PMB) coating, was reported to inhibit sucrose-dependent biofilm formation by Streptococcus mutans, a cariogenic bacterium, on the surface of poly(methyl methacrylate) (PMMA) denture bases. However, S. mutans is a minor component of the oral microbiome and does not play an important role in biofilm formation in the absence of sucrose. Other, more predominant oral streptococci must play an indispensable role in sucrose-independent biofilm formation. In the present study, the effect of PMB coating on PMMA was evaluated using various oral streptococci that are known to be initial colonizers during biofilm formation on tooth surfaces. PMB coating on PMMA drastically reduced sucrose-dependent tight biofilm formation by two cariogenic bacteria (S. mutans and Streptococcus sobrinus), among seven tested oral streptococci, as described previously [N. Takahashi, F. Iwasa, Y. Inoue, H. Morisaki, K. Ishihara, K. Baba, J. Prosthet. Dent. 112 (2014) 194–203]. Streptococci other than S. mutans and S. sobrinus did not exhibit tight biofilm formation even in the presence of sucrose. On the other hand, all seven species of oral streptococci exhibited distinctly reduced glucose-dependent soft biofilm retention on PMB-coated PMMA. We conclude that PMB coating on PMMA surfaces inhibits biofilm attachment by initial colonizer oral streptococci, even in the absence of sucrose, indicating that PMB coating may help maintain clean conditions on PMMA surfaces in the oral cavity..
2. Kunio Ishikawa, Development of carbonate apatite as artificial bone substitute, Japanese Magazine of Mineralogical and Petrological Sciences, 10.2465/gkk.161217, 46, 1, 42-46, 2017, Carbonate apatite (CO3Ap) that contains 6-9 wt% carbonate in apatitic structure is the composition of human bone. Unfortunately, CO3Ap cannot be sintered due to the presence of CO3. CO3Ap block was found to be fabricated based on a compositional transformation though dissolution-precipitation reaction using a precursor such as calcite block. CO3Ap block thus fabricated up-regulate the differentiation of osteoblastic cells and resorbed by the osteoclasts. As results of these response to cells, CO3Ap block demonstrate much better osteoconductivity than hydroxyapatite [HAp: Ca10(PO4)6(OH)2)] which is currently used artificial bone substitute. Also, CO3Ap block was found to be replace to new bone similar to autograft even though HAp would not replace to bone. Clinical trial of CO3Ap is now in progress, and CO3Ap will be available for its clinical use next year..
3. Naoyuki Fukuda, Kanji Tsuru, Yoshihide Mori, Kunio Ishikawa, Effect of citric acid on setting reaction and tissue response to β-TCP granular cement, Biomedical Materials (Bristol), 10.1088/1748-605X/aa5aea, 12, 1, 2017.02, We recently reported that when an acidic calcium phosphate solution is mixed with β-tricalcium phosphate (β-TCP) granules, the resulting dicalcium phosphate dihydrate (DCPD) crystals form bridges between the β-TCP granules, creating a set interconnected porous structure in approximately 1 min. Although this self-setting β-TCP granular cement (β-TCPGC) is useful for clinical applications, the short setting time is a key drawback for handling. In this study, the setting time of β-TCPGC was adjusted with the addition of citric acid, which is a known inhibiter of DCPD crystal growth. As the concentration of citric acid in the acidic calcium phosphate solution increased, the amount of DCPD formation in the set β-TCPGC decreased, and the crystal morphology of DCPD became elongated. β-TCPGC prepared with various citric acid concentrations were used as grafting material in rat calvarial bone defects to evaluate bone regeneration in vivo. Four weeks after implantation, no inflammatory reaction and approximately 20% new bone formation were observed, regardless of the presence or absence of citric acid in the liquid phase of β-TCPGC. We concluded, therefore, that citric acid might be a useful retarder of β-TCPGC setting times..
4. Kanji Tsuru, Ayami Yoshimoto, Masayuki Kanazawa, Yuki Sugiura, Yasuharu Nakashima, Kunio Ishikawa, Fabrication of carbonate apatite block through a dissolution-precipitation reaction using calcium hydrogen phosphate dihydrate block as a precursor, Materials, 10.3390/ma10040374, 10, 4, 2017.03, Carbonate apatite (CO3Ap) block, which is a bone replacement used to repair defects, was fabricated through a dissolution-precipitation reaction using a calcium hydrogen phosphate dihydrate (DCPD) block as a precursor. When the DCPD block was immersed in NaHCO3 or Na2CO3 solution at 80 °C, DCPD converted to CO3Ap within 3 days. β-Tricalcium phosphate was formed as an intermediate phase, and it was completely converted to CO3Ap within 2 weeks when the DCPD block was immersed in Na2CO3 solution. Although the crystal structures of the DCPD and CO3Ap blocks were different, the macroscopic structure was maintained during the compositional transformation through the dissolution-precipitation reaction. CO3Ap block fabricated in NaHCO3 or Na2CO3 solution contained 12.9 and 15.8 wt % carbonate, respectively. The diametral tensile strength of the CO3Ap block was 2 MPa, and the porosity was approximately 57% regardless of the carbonate solution. DCPD is a useful precursor for the fabrication of CO3Ap block..
5. Kunio Ishikawa, Giichiro Kawachi, Kanji Tsuru, Ayami Yoshimoto, Fabrication of calcite blocks from gypsum blocks by compositional transformation based on dissolution–precipitation reactions in sodium carbonate solution, Materials Science and Engineering C, 10.1016/j.msec.2016.11.093, 72, 389-393, 2017.03, Calcium carbonate (CaCO3) has been used as a bone substitute, and is a precursor for carbonate apatite, which is also a promising bone substitute. However, limited studies have been reported on the fabrication of artificial calcite blocks. In the present study, cylindrical calcite blocks (ϕ6 × 3 mm) were fabricated by compositional transformation based on dissolution–precipitation reactions using different calcium sulfate blocks as a precursor. In the dissolution–precipitation reactions, both CaSO4·2H2O and CaSO4 transformed into calcite, a polymorph of CaCO3, while maintaining their macroscopic structure when immersed in 1 mol/L Na2CO3 solution at 80 °C for 1 week. The diametral tensile strengths of the calcite blocks formed using CaSO4·2H2O and CaSO4 were 1.0 ± 0.3 and 2.3 ± 0.7 MPa, respectively. The fabrication of calcite blocks using CaSO4·2H2O and CaSO4 proposed in this investigation may be a useful method to produce calcite blocks because of the self-setting ability and high temperature stability of gypsum precursors..
6. Khairul Anuar Shariff, Kanji Tsuru, Kunio Ishikawa, Fabrication of dicalcium phosphate dihydrate-coated β-TCP granules and evaluation of their osteoconductivity using experimental rats, Materials Science and Engineering C, 10.1016/j.msec.2017.03.004, 75, 1411-1419, 2017.06, β-Tricalcium phosphate (β-TCP) has attracted much attention as an artificial bone substitute owing to its biocompatibility and osteoconductivity. In this study, osteoconductivity of β-TCP bone substitute was enhanced without using growth factors or cells. Dicalcium phosphate dihydrate (DCPD), which is known to possess the highest solubility among calcium phosphates, was coated on β-TCP granules by exposing their surface with acidic calcium phosphate solution. The amount of coated DCPD was regulated by changing the reaction time between β-TCP granules and acidic calcium phosphate solution. Histomorphometry analysis obtained from histological results revealed that the approximately 10 mol% DCPD-coated β-TCP granules showed the largest new bone formation compared to DCPD-free β-TCP granules, approximately 2.5 mol% DCPD-coated β-TCP granules, or approximately 27 mol% DCPD-coated β-TCP granules after 2 and 4 weeks of implantation. Based on this finding, we demonstrate that the osteoconductivity of β-TCP granules could be improved by coating their surface with an appropriate amount of DCPD..
7. Masayuki Kanazawa, Kanji Tsuru, Naoyuki Fukuda, Yuta Sakemi, Yasuharu Nakashima, Kunio Ishikawa, Evaluation of carbonate apatite blocks fabricated from dicalcium phosphate dihydrate blocks for reconstruction of rabbit femoral and tibial defects, Journal of Materials Science: Materials in Medicine, 10.1007/s10856-017-5896-5, 28, 6, 2017.06, Abstract: This study aimed to evaluate in vivo behavior of a carbonate apatite (CO3Ap) block fabricated by compositional transformation via a dissolution–precipitation reaction using a calcium hydrogen phosphate dihydrate [DCPD: CaHPO4·2H2O] block as a precursor. These blocks were used to reconstruct defects in the femur and tibia of rabbits, using sintered dense hydroxyapatite (HAp) blocks as the control. Both the CO3Ap and HAp blocks showed excellent tissue response and good osteoconductivity. HAp block maintained its structure even after 24 weeks of implantation, so no bone replacement of the implant was observed throughout the post-implantation period in either femoral or tibial bone defects. In contrast, CO3Ap was resorbed with increasing time after implantation and replaced with new bone. The CO3Ap block was resorbed approximately twice as fast at the metaphysis of the proximal tibia than at the epiphysis of the distal femur. The CO3Ap block was resorbed at an approximately linear change over time, with complete resorption was estimated by extrapolation of data at approximately 1−1.5 years. Hence, the CO3Ap block fabricated in this study has potential value as an ideal artificial bone substitute because of its resorption and subsequent replacement by bone..
8. Wee Keat Cheah, Kunio Ishikawa, Radzali Othman, Fei Yee Yeoh, Nanoporous biomaterials for uremic toxin adsorption in artificial kidney systems
A review, Journal of Biomedical Materials Research - Part B Applied Biomaterials, 10.1002/jbm.b.33475, 105, 5, 1232-1240, 2017.07, Hemodialysis, one of the earliest artificial kidney systems, removes uremic toxins via diffusion through a semipermeable porous membrane into the dialysate fluid. Miniaturization of the present hemodialysis system into a portable and wearable device to maintain continuous removal of uremic toxins would require that the amount of dialysate used within a closed-system is greatly reduced. Diffused uremic toxins within a closed-system dialysate need to be removed to maintain the optimum concentration gradient for continuous uremic toxin removal by the dialyzer. In this dialysate regenerative system, adsorption of uremic toxins by nanoporous biomaterials is essential. Throughout the years of artificial kidney development, activated carbon has been identified as a potential adsorbent for uremic toxins. Adsorption of uremic toxins necessitates nanoporous biomaterials, especially activated carbon. Nanoporous biomaterials are also utilized in hemoperfusion for uremic toxin removal. Further miniaturization of artificial kidney system and improvements on uremic toxin adsorption capacity would require high performance nanoporous biomaterials which possess not only higher surface area, controlled pore size, but also designed architecture or structure and surface functional groups. This article reviews on various nanoporous biomaterials used in current artificial kidney systems and several emerging nanoporous biomaterials..
9. Yuki Sugiura, Kanji Tsuru, Kunio Ishikawa, “Fabrication of arbitrarily shaped carbonate apatite foam based on the interlocking process of dicalcium hydrogen phosphate dihydrate”, Journal of Materials Science: Materials in Medicine, 10.1007/s10856-017-5937-0, 28, 8, 2017.08, Carbonate apatite (CO3Ap) foam with an interconnected porous structure is highly attractive as a scaffold for bone replacement. In this study, arbitrarily shaped CO3Ap foam was formed from α-tricalcium phosphate (α-TCP) foam granules via a two-step process involving treatment with acidic calcium phosphate solution followed by hydrothermal treatment with NaHCO3. The treatment with acidic calcium phosphate solution, which is key to fabricating arbitrarily shaped CO3Ap foam, enables dicalcium hydrogen phosphate dihydrate (DCPD) crystals to form on the α-TCP foam granules. The generated DCPD crystals cause the α-TCP granules to interlock with each other, inducing an α-TCP/DCPD foam. The interlocking structure containing DCPD crystals can survive hydrothermal treatment with NaHCO3. The arbitrarily shaped CO3Ap foam was fabricated from the α-TCP/DCPD foam via hydrothermal treatment at 200 °C for 24 h in the presence of a large amount of NaHCO3..
10. I. Grigoraviciute-Puroniene, K. Tsuru, E. Garskaite, Z. Stankeviciute, A. Beganskiene, Kunio Ishikawa, A. Kareiva, A novel wet polymeric precipitation synthesis method for monophasic β-TCP, Advanced Powder Technology, 10.1016/j.apt.2017.06.014, 28, 9, 2325-2331, 2017.09, β-Tricalcium phosphate (β-Ca3(PO4)2, β-TCP) powders were synthesized using wet polymeric precipitation method for the first time to our best knowledge. The results of X-ray diffraction analysis showed the formation of almost single a Ca-deficient hydroxyapatite (CDHA) phase of a poor crystallinity already at room temperature. With continuously increasing the calcination temperature up to 800 °C the crystalline β-TCP was obtained as the main phase. It was demonstrated that infrared spectroscopy is very effective method to characterize the formation of β-Ca3(PO4)2. The SEM results showed that β-Ca3(PO4)2 solids were homogeneous having a small particle size distribution. The β-TCP powders consisted of spherical particles varying in size from 100 to 300 nm. Fabricated β-TCP specimens were placed to the bones of the rats and maintained for 1–2 months. The histological properties of these samples will be also investigated..
11. Tya Indah Arifta, Melvin L. Munar, Kanji Tsuru, Kunio Ishikawa, Fabrication of interconnected porous calcium-deficient hydroxyapatite using the setting reaction of α tricalcium phosphate spherical granules, Ceramics International, 10.1016/j.ceramint.2017.05.162, 43, 14, 11149-11155, 2017.10, Interconnected porous calcium-deficient hydroxyapatite (cdHAp) blocks may be an ideal biomaterial to repair bone defects because of their greater similarity to human bone than that of sintered hydroxyapatite (HAp) with respect to calcium content and crystallinity. In particular, the interconnected pores in cdHAp may provide pathways for cell migration and tissue ingrowth. In this study, the feasibility of fabricating interconnected porous cdHAp blocks through the setting reaction of alpha-tricalcium phosphate (αTCP) spherical granules was investigated. It was found that regulation of cdHAp formation was important to fabricate interconnected porous cdHAp blocks. That is, cdHAp needed to precipitate preferentially at the contacting areas between αTCP spherical granules. Exposure of αTCP spherical granules to steam under appropriate pressure was effective for this purpose. When αTCP spherical granules were immersed in water at 100 °C, the setting reaction resulted in dense cdHAp blocks because of the free crystal growth of cdHAp in water. Therefore, steam was used to localize the water at the contacting areas between αTCP spherical granules, which was driven by the surface tension of the water. Without an applied load, no setting reaction was observed when αTCP spherical granules were exposed to steam at 100 °C for 12 h. In contrast, under a load of 20 MPa, cdHAp precipitated to bridge spherical granules, providing an interconnected porous cdHAp block. The porosity and diametral tensile strength of this block were approximately 63% and 1.5 MPa, respectively..
12. Naoyuki Fukuda, Kanji Tsuru, Yoshihide Mori, Kunio Ishikawa, Fabrication of self-setting β-tricalcium phosphate granular cement, Journal of Biomedical Materials Research - Part B Applied Biomaterials, 10.1002/jbm.b.33891, 106, 2, 800-807, 2018.02, Bone defect reconstruction would be greatly improved if β-tricalcium phosphate (β-TCP) granules had the ability to self-set without sacrificing their osteoconductivity potential. This study aimed to identify a method to permit β-TCP self-setting whilst maintaining good osteoconductivity. When mixed with acidic calcium phosphate solution, β-TCP granules were found to readily set, forming a fully interconnected porous structure. On mixing, dicalcium phosphate dihydrate crystals formed on the surface of β-TCP granules, bridging the granules and resulting in the setting reaction. The setting time of the β-TCP granular cement (β-TCP GC) was approximately 1 min and its mechanical strength, in terms of diametral tensile strength, was approximately 0.8 MPa. The β-TCP GC and β-TCP granules both showed the same level of osteoconductivity within rat calvaria bone defects. At 2 and 4 weeks post-implantation, new bone formation was comparable between the two β-TCP based bone substitutes. We conclude that β-TCP GC has excellent potential for use as a cement in bone defect reconstruction..
13. Yuki Sugiura, Melvin L. Munar, Kunio Ishikawa, Fabrication of octacalcium phosphate foam through phase conversion and its histological evaluation, Materials Letters, 10.1016/j.matlet.2017.10.051, 212, 28-31, 2018.02, Octacalcium phosphate (OCP) foam with an interconnected porous structure was fabricated through phase conversion via a dissolution–precipitation reaction using calcium sulfate hemihydrate (CSH: CaSO4·1/2H2O) granules as precursors in a sodium dihydrogen phosphate (NaDP: NaH2PO4) solution. The diametral tensile strength and porosity of the OCP foam were 0.15 ± 0.04 MPa and 69.4% ± 0.04%, respectively. When the OCP foam was implanted into bone defects in a rabbit femur, the OCP foam showed an excellent tissue response, and the bone penetrated into the porous structure. The osteoconductivity and bone-replacement rate were significantly higher than those of an OCP compact..
14. Kunio Ishikawa, Tansza Setiana Putri, Akira Tsuchiya, Keisuke Tanaka, Kanji Tsuru, Fabrication of interconnected porous β-tricalcium phosphate (β-TCP) based on a setting reaction of β-TCP granules with HNO3 followed by heat treatment, Journal of Biomedical Materials Research - Part A, 10.1002/jbm.a.36285, 106, 3, 797-804, 2018.03, β-Tricalcium phosphate [β-TCP] is the typical bone substitute due to its excellent osteoconductivity and bioresorbability. One of the keys to improve its potential as bone substitute is to introduce porous structure and its regulation. In this study, interconnected porous β-TCP blocks were fabricated through a setting reaction of β-TCP granules and subsequent heat treatment. First, β-TCP granules were mixed with HNO3. Upon mixing, β-TCP granules were bridged with dicalcium phosphate dihydrate [DCPD: CaHPO4·2H2O] containing Ca(NO3)2. Then, the DCPD-bridged β-TCP was heated at 1100°C. During the heating process, DCPD containing Ca(NO3)2 transformed into β-TCP and bonded with β-TCP granules. As a result, an interconnected porous β-TCP block formed. The diametral tensile strength and porosity of the interconnected porous β-TCP block fabricated from 200–300-μm β-TCP granules and 5 N HNO3 and then heated at 1,100°C were 1.4 ± 0.2 MPa and 57% ± 2%, respectively..
15. Toshiyuki Suge, Shingo Shibata, Kunio Ishikawa, Takashi Matsuo, Fluoride activity of antibacterial ammonium hexafluorosilicate solution for the prevention of dentin caries, American Journal of Dentistry, 31, 2, 103-106, 2018.04, Purpose: To evaluate the acid resistance of various antibacterial ammonium hexafluorosilicate (SiF) solutions. Methods: Antibacterial SiF solutions were prepared with the addition of chlorhexidine (CHX), cetylpyridinium chloride (CPC), isopropyl methylphenol (IPMP), or epigallocatechin gallate (EGCG). Hydroxyapatite pellets were treated with SiF solution with or without antibacterial agents for 3 minutes. The demineralized depth of hydroxyapatite pellets after SiF treatment was measured using a surface roughness analyzer. Results: SiF+CPC solution showed equivalent acid resistance to SiF and AgF treatment. In contrast, the original acid resistance activity of SiF solution was diminished by the addition of other antibacterial agents (CHX, IPMP and EGCG). SiF with the addition of CPC was the most effective for reducing the demineralized depth, showing the same levels as those of SiF and AgF..
16. Sunarso, Akira Tsuchiya, Naoyuki Fukuda, Riki Toita, Kanji Tsuru, Kunio Ishikawa, Effect of micro-roughening of poly(ether ether ketone) on bone marrow derived stem cell and macrophage responses, and osseointegration, Journal of Biomaterials Science, Polymer Edition, 10.1080/09205063.2018.1461448, 1-14, 2018.04, Poly(ether ether ketone) (PEEK) has emerged as a candidate to replace metal implants because of its satisfactory mechanical properties, radiolucency, and lack of metal allergy. However, PEEK lacks osseointegration ability limiting its clinical applications. To overcome this problem, we prepared PEEK with a micro-rough surface using the sandblast method to modulate its osseointegration property; the sandblast method is simple, cost-effective, and is already applied to clinical metal implants. The surface roughness of the sandblasted PEEK was about 2.3 μm, whereas that of mirror-polished PEEK was 0.06 μm. Rat bone marrow-derived mesenchymal stem cells (RMSCs) showed higher proliferation, osteocalcin (OC) expression and bone-like nodule formation on micro-roughened PEEK compared with those cultured on mirror-polished PEEK, suggesting that micro-roughening facilitated RMSCs proliferation and differentiation. The micro-roughened surface slightly mitigated secretion of inflammatory C-C motif chemokine 2 (CCL-2) from lipopolysaccharide (LPS)-stimulated macrophages, but not of tumor necrosis factor α (TNFα) and interleukin-6 (IL-6). Finally, to compare osseointegration, specimens were implanted in rat femur bone marrow cavities, and then the pull-out force was measured. The pull-out force of micro-roughened PEEK was about four times higher than that of the mirror-polished PEEK. These results showed that micro-roughening of PEEK using the sandblast method was able to improve osseointegration, partly through elevating proliferation and differentiation of RMSCs..
17. Yuki Sugiura, Kunio Ishikawa, Effect of calcium and phosphate on compositional conversion from dicalcium hydrogen phosphate dihydrate blocks to octacalcium phosphate blocks, Crystals, 10.3390/cryst8050222, 8, 5, 2018.05, Octacalcium phosphate (OCP) has attracted much attention as an artificial bone substitute because of its excellent osteoconductive and bone replacement properties. Although numerous studies have investigated OCP powder fabrication, there are only a few studies on OCP block fabrication. Therefore, in this study, the feasibility of optimizing dicalcium hydrogen phosphate dihydrate (DCPD) blocks, as a precursor for OCP block fabrication, under a pH 6 adjusted acetate buffer solution at 70 C for 2 days was investigated. When a DCPD block was immersed in acetate buffer, the block was partially converted to OCP, with a large amount of dicalcium hydrogen phosphate anhydrate (DCPA), and its macroscopic structure was maintained. When the DCPD block was immersed in a Ca-containing solution, it was converted to mainly hydroxyapatite (HAp) with DCPA. On the other hand, when the DCPD block was immersed in a PO4-containing solution, the block was converted to OCP, and its macroscopic structure was maintained. In other words, the PO4-induced calcium phosphate with a Ca/P molar ratio lower than 1.0 may represent an intermediate phase during the compositional transformation from a DCPD block to an OCP block through the dissolution–precipitation reaction..
18. Sunarso, Riki Toita, Kanji Tsuru, Ishikawa Kunio, A superhydrophilic titanium implant functionalized by ozone gas modulates bone marrow cell and macrophage responses., J Mater Sci: Mater Med, 10.1007/s10856-016-5741-2, 68, 291-298, 2016.08.
19. Sunarso, Riki Toita, Kanji Tsuru, Ishikawa Kunio, Immobilization of calcium and phosphate ions improves the osteoconductivity of titanium implants., Mater Sci Eng C, org/10.1016/j.msec.2016.05.090, 68, 291-298, 2016.11.
20. Yuki Sugiura, Kanji Tsuru, Ishikawa Kunio, Fabrication of carbonate apatite pseudomorph from highly soluble acidic calcium phosphate salts through carbonation., J Ceram Soc Jpn, 10.2109/jcersj2.16046, 124, 8, 827-832, 2016.08.
21. Ishikawa Kunio, Koga Noriko, Kanji Tsuru, Ichiro Takahashi, Fabrication of interconnected porous calcite by bridging calcite granules with dicalcium phosphate dihydrate and their histological evaluation., J Biomed Mater Res: Part A, 10.1002/jbm.a.35604, 104, 3, 652-658, 2016.05.
22. Masaaki Takechi, Yoshiaki Ninomiya, Kouji Ohta, Misato Tada, Kazuki Sasaki, Mohammad Zeshaan Rahman, Akira Ohta, Kanji Tsuru, Ishikawa Kunio, Effects of apatite cement containing atelocollagen on attachment to, and proliferation and differentiation of MC3T3-E1 osteoblastic cells., Materials, 10.3390/ma9040283, 9, 4, 283-283, 2016.04.
23. Koga Noriko, Ishikawa Kunio, Kanji Tsuru, Ichiro Takahashi, Effects of acidic calcium phosphate concentration on mechanical strength of porous calcite fabricated by bridging with dicalcium phosphate dihydrate., Ceram Int, org/10.1016/j.ceramint.2015.12.017, 42, 7, 7912-7917, 2016.05.
24. 176. Siti Noorzidah Mohd Sabri, Synzana Abu Bakar, Abdul Yazid Abdul Manaf, Siti Frahana Hisham, Mohd Azmirrudin Ahmed, K. Jamuna Thevi, Kartini Norrsal, Hairul Anuar Shafiff, Ishikawa Kunio, Phase transformation of biphasic granules of gypsum and carbonated apatite at low temperatures., Adv Mater Res, 10.4028/www.scientific.net/AMR.1133.50 , 1133, 50-54, 2016.01.
25. Yuki Sugiura, Kanji Tsuru, Ishikawa Kunio, Fabrication of carbonate apatite foam based on the setting reaction of α-tricalcium phosphate foam granules., Ceram Int, org/10.1016/j.ceramint.2015.08.081, 42, 204-210, 2016.01.
26. Shi Xingling, Xu L, Le Bang Thi, Zhou G, Zheng C, Kanji Tsuru, Ishikawa Kunio, Partial oxidation of TiN coating by hydrothermal treatment and ozone treatment to improve its osteoconductivity, Materials Science and Engineering: C, 10.1016/j.msec.2015.10.024, 59, 542-548, 2016.02.
27. Khairul Anuar Shariff, Kanji Tsuru, Ishikawa Kunio, Fabrication of interconnected pore forming α-tricalcium phosphate foam granules cement., J Biomat Appl, 10.1177/0885328215601939, 30, 6, 838-845, 2016.06.
28. Riki Toita, Sunarso, Ahmed Nafis Rashid, Kanji Tsuru, Ishikawa Kunio, Modulation of the osteoconductive property and immune response of poly(ether ether ketone) by modification with calcium ions., J Mater Chem B, 10.1039/C5TB01679G, 3, 44, 8738-8746, 2015.10.
29. Shi Xing Ling, Xu L, MUNAR MELVIN DE LEON, Ishikawa Kunio, Hydrothermal treatment for TiN as abrasion resistant dental implant coating and its fibroblast response., Materials Science and Engineering: C, 10.1016/j.msec.2014.12.059, 49, 1-6, 2015.04.
30. Kanji Tsuru, Ruslin, Shigeki Matsuya, Ishikawa Kunio, Effects of the method of apatite seed crystals addition on setting reaction of α-tricalcium phosphate based apatite cement., J Mater Sci: Mater Med, 10.1007/s10856-015-5570-8, 26, 244-451, 2015.09.
31. Yasunori Ayukawa, Yumiko Suzuki, Takamori Y, Atsuta I, Nakamura H, Sawase T, Koyano K, Hara Y., Ishikawa Kunio, Kanji Tsuru, Histological comparison in rats between carbonate apatite fabricated from gypsum and sintered hydroxyapatite on bone remodeling., BioMed Res Int, 10.1155/2015/579541, Article ID 579541, 2015.07.
32. Trung-Kien P, Ishikawa Kunio, Kanji Tsuru, Setting reaction of α-TCP spheres and an acidic calcium phosphate solution for the fabrication of fully interconnected macroporous calcium phosphate., Ceram Int, 10.1016/j.ceramint.2015.07.146, 41, 10, 13525-13531, 2015.12.
33. ALIREZA VALANEZHAD, Kanji Tsuru, Ishikawa Kunio, Fabrication of strongly attached hydroxyapatite coating on titanium by hydrothermal treatment of Ti-Zn-PO4 coated titanium in CaCl2 solution., J Mater Sci: Mater Med, 10.1007/s10856-015-5548-6, 26, 7, 5548-5557, 2015.07.
34. Taro Nikaido, Kanji Tsuru, MUNAR MELVIN DE LEON, Michito Maruta, Shigeki Matsuya, Seiji Nakamura, Ishikawa Kunio, Fabrication of β-TCP foam: effects of magnesium oxide as phase stabilizer on its properties., Ceram Int, 41, 14245-14250, 2015.07.
35. Cahyanto A, Maruta M, Kanji Tsuru, Matsuya S, Ishikawa Kunio, Fabrication of bone cement that fully transforms to carbonate apatite., Dent Mater J, 34, 3, 394-401, 2015.06.
36. Noriko Koga, Kanji Tsuru, Ichiro Takahashi, Ishikawa Kunio, Effects of humidity on calcite block fabrication using calcium hydroxide compact. , Ceram Int, 41, 8, 9482-9487, 2015.09.
37. Ishikawa Kunio, Nagai H, Fujioka-Kobayashi M, Fujisawa K, Ohe G, Takamaru N, Hara K, Uchida D, Tamatani T, Miyamoto Y, Effects of low crystalline carbonate apatite on proliferation and osteoblastic differentiation of human bone marrow cells., J Mater Sci: Mater Med, 26, 2, 99-107, 2015.02.
38. Ishikawa Kunio, Nguyen Xuan Thanh Tram, Kanji Tsuru, Toita Riki, Fabrication of porous calcite using chopped nylon fiber and its evaluation using rats., J Mater Sci: Mater Med, 26, 2, 94-101, 2015.02.
39. Rashid RN, Tsuru Kanji, Ishikawa Kunio, Effect of calcium-ozone treatment on chemical and biological properties of polyethylene terephthalate., J Biomed Mater Res: Part B, 3, 853-860, 2015.08.
40. Munar GM, MUNAR MELVIN DE LEON, Tsuru Kanji, Ishikawa Kunio, Effects of PLGA reinforcement methods on the mechanical property of carbonate apatite foam. , Bio-Medical Materials and Engineering, 24, 5, 1817-1825, 2014.05.
41. Nomura S, Tsuru Kanji, Matsuya S, Ichiro Takahashi, Ishikawa Kunio, Fabrication of carbonate apatite block from set gypsum based on dissolution-precipitation reaction in phosphate-carbonate mixed solution., Dent Mater J, 33(2): 166-172,, 33, (2): , 166-172, 2014.03.
42. Ishikawa Kunio, Tsuru Kanji, Ichiro Takahashi, Fabrication of carbonate apatite block from set gypsum based on dissolution-precipitation reaction in phosphate-carbonate mixed solution., Dental Materials Journal, 33, (2), 166-172, 2014.03.
43. Munar GM, Munar ML, Tsuru Kanji, Ishikawa Kunio, Influence of PLGA concentrations on structural and mechanical properties of carbonate apatite foam., Dent Mater J, 32(4):608-614, 32, (4):, 608--614, 2013.07.
44. Bang LT, Tsuru Kanji, Munar M, Ishikawa Kunio, Othman R, Mechanical behavior and cell response of PCL coated α-TCP foam for cancellous-type bone replacement. , Ceram Int, 39(5): 5631-5637, 39, (5): , 5631--5637, 2013.07.
45. Xingling Shi, Tsuru Kanji, Lingli Xu, Giichiro Kawachi, Ishikawa Kunio, Effects of solution pH on the structure and biocompatibility of Mg-containing TiO2 layer fabricated on titanium by hydrothermal treatment. , Applied Surface Science Vols.270, 270, 445-451, 2013.04.
46. Toshiyuki Suge, Kunio Ishikawa, Takashi Matsuo, Changes in the crystallinity of hydroxyapatite powder and structure of enamel treated with several concentrations of ammonium hexafluorosilicate., American Journal of Dentistry, 25(5): 299-302, 2012., 2012.10.
47. Akihiro Otsu, Kanji Tsuru, Michito Maruta, Melvin L. Munar, Shigeki Matsuya, Kunio Ishikawa: , Fabrication of microporous calcite block from calcium hydroxide compact under carbon dioxide atmosphere at high temperature., Dental Materials Journal, 31(4): 593-600, 2012., 2012.07.
48. Nurazreena Ahmad, Kanji Tsuru, Melvin L. Munar, Michito Maruta, Shigeki Matsuya, Kunio Ishikawa, Effect of precursor’s solubility on the mechanical property of hydroxyapatite formed by dissolution-precipitation reaction of tricalcium phosphate. , Dental Materials Journal, 31(4): 593-600, 2012., 2012.07.
49. Kazuhiro Sunouchi, Kanji Tsuru, Michito Maruta, Giichiro Kawachi, Shigeki Matsuya, Yoshihiro Terada, Kunio Ishikawa, Fabrication of solid and hollow carbonate apatite microspheres as bone substitutes using calcite microspheres as a precursor., Dental Materials Journal, 31(4): 549-557, 2012., 2012.07.
50. Kawashita M, Taninai K, Li Z, Ishikawa K,•Yoshida Y, Preparation of low-crystalline apatite nanoparticles and their coating onto quartz substrates., J Mater Sci: Mater Med, 23: 1355-1362, 2012., 2012.06.
51. Shi X, Nakagawa M, Kawachi G, Xu L, Ishikawa K, Surface modification of titanium by hydrothermal treatment in Mg-containing solution and early osteoblast responses., J Mater Sci: Mater Med, 23:1281-1290, 2012., 2012.06.
52. Sakai A, Valanezahad A, Ozaki M, Ishikawa K, Matsuya S, Preparation of Sr-containing carbonate apatite as a bone substitute and its properties., Dent Mater J, 31(2): 197-205, 2012., 2012.04.
53. Shibata S, Suge T, Kimura T, Ishikawa K, Matsuo T, Antibacterial activity of ammonium hexafluorosilicate solution with antimicrobial agents for the prevention of dentin caries., Am J Dent, 31(2): 197-205, 2012., 2012.02.
54. Ishikawa Kunio, Kanji Tsuru, Fabrication of solid and hollow carbonate apatite microspheres as bone substitutes using calcite microspheres as a precursor., Dental Materials Journal, 31(4): 549-557, 2012., 2012.01.
55. Valanezahad A, Tsuru K, Maruta M, Kawachi G, Matsuya S, Ishikawa K, A new biocompatible coating layer applied on titanium substrates using a modified zinc phosphatizing method., Surafce & Coating Tech, 206: 2207-2212, 2012., 2012.01.
56. Bang LT, Ishikawa K, Othman R, Effect of silicon and heat-treatment temperature on the morphology and mechanical properties of silicon - substituted hydroxyapatite., Ceram Int, 37(8): 3637-3642, 2011., 2011.12.
57. Valanezahad A, Ishikawa K, Tsuru K, Maruta M, Matsuya S, Hydrothermal calcium modification of 316L stainless steel and its apatite forming ability in simulated body fluid., Dent Mater J, 30(5), 749–753, 2011., 2011.10.
58. Mano T, Ishikawa K, Harada K, Umeda H, Ueyama Y, Comparison of apatite-coated titanium prepared by blast coating and flame spray methods -Evaluation using simulated body fluid and initial histological study, Dent Mater J, 30(4), 431-437, 2011., 2011.07.
59. Shibata S, Suge T, Ishikawa K, Matsuo T, Occlusion of dentin tubules with antibacterial ammonium hexafluorosilicate solution for the prevention of dentin caries., Am J Dent, 24: 148-152, 2011., 2011.06.
60. Maruta M, Matsuya S, Nakamura S, Ishikawa K, Fabrication of low-crystalline carbonate apatite foam bone replacement based on phase transformation of calcite foam., Dent Mater J, 30(1): 14-20, 2011., 2011.02.
61. Kien PT, Maruta M, Tsuru K, Matsuya S, Ishikawa K, Effect of phosphate solution on setting reaction of α-TCP spheres. , J Austrarian Ceram Soc, 46(2):63-67, 2010., 2010.10.
62. Valanezhad A, Tsuru K, Maruta M, Kawachi G, Matsuya S, Ishikawa K, Zinc phosphate coating on 316L-type stainless steel using hydrothermal treatment., Surafce & Coating Tech, 205: 2538–2541, 2010., 2010.10.
63. Daitou F, Maruta M, Kawachi G, Tsuru K, Matsuya S, Terada Y, Ishikawa K, Fabrication of carbonate apatite block based on internal dissolution-precipitation reaction of dicalcium phosphate and calcium carbonate., Dent Mater J, 29(3): 303-308, 2010, 2010.06.
64. Matsumoto K, Tsuru K, Kawachi G, Maruta M, Matsuya S, Takahashi I, Ishikawa K, Reinforcement of carbonate apatite bone substitutes with carbonate apatite by Ca salt introduction., J Ceram Soc Jpn, 118(6): 521-524,2010., 2010.06.
65. Ishikawa K, Matsuya S, Lin X, Zhang L, Yuasa T, Miyamoto Y, Fabrication of low crystalline B-type carbonate apatite block from low crystalline calcite block, J Ceram Soc Jpn, 118(5): 341-344, 2010, 2010.05.
66. Tarique AAM, Tsuru K, Maruta M, Takeuchi A, Matsuya S, Terada Y, Ishikawa K, In vitro osteoconductivity evaluation of alumina treated hydrothermally in CaCl2 solution., J Ceram Soc Jpn, 118(6): 512-515, 2010, 2010.05.
67. Zhang L, Ayukawa Y, LeGeros RZ, Matsuya S, Koyano K, Ishikawa K, Tissue-response to calcium-bonded titanium surface, J Biomed Mater Res: Part A, in press, 2010.05.
68. Udoh K, Munar ML, Maruta M, Matsuya S, Ishikawa K, Effects of sintering temperature on physical and compositional properties of α-tricalcium phosphate foam, Dent Mater J, 29(2): 154-159, 2010., 2010.03.
69. Suge T, Kawasaki A, Ishikawa K, Matsuo T, Ebisu S, Effects of ammonium hexafluorosilicate concentration on dentin tubule occlusion and composition of the precipitate, Dent Mater, 26(1): 29-34, 2010, 2010.01.
70. Shimogoryo R, Eguro T, Kimura E, Maruta M, Matsuya S, Ishikawa K, Effects of added mannitol on the setting reaction and mechanical strength of apatite cement, Dent Mater J, 28(5): 627-633, 2009., 2009.09.
71. Lowmunkong R, Sohmura T, Suzuki Y, Matsuya S, Ishikawa K, Fabrication of freeform bone-filling calcium phosphate ceramics by gypsum 3D printing method, J Biomed Mater Res B: Appl Biomater, 90(2), 531-539, 2009., 2009.08.
72. Takeuchi A, Munar ML, Wakae H, Maruta M, Matsuya S, Tsuru K, Ishikawa K, Effect of temperature on crystallinity of carbonate apatite foam prepared from α-tricalcium phosphate by hydrothermal treatment, Bio-Med Mater Eng, 19(2-3), 205–211, 2009, 2009.07.
73. Karashima S, Takeuchi A, Matsuya S, Udoh K, Koyano K, Ishikawa K, Fabrication of low-crystallinity hydroxyapatite foam based on the setting reaction of alpha-tricalcium phosphate foam, J Biomed Mater Res A. 88(3):628-633, 2009, 2009.03.
74. Zaman CT, Takeuchi A, Matsuya S, Zaman Q. H. M. S, Ishikawa K, abrication of B-type carbonate apatite blocks by the phosphorization of free-molding gypsum-calcite composite, Dent Mater J, 27(5): 710-715, 2008., 2008.05.
75. Kunio Ishikawa, Shigeki Matsuya, Masaharu Nakagawa, Koh-ichi Udoh and Kazuomi Suzuki, Basic properties of apatite cement containing spherical tetracalcium phosphate made with plasma melting method., Journal of Materials Science: Materials in Medicine, 10.1023/B:JMSM.0000010092.01661.a8, 15, 1, 13-17, 15(1), 13-17, 2004.01.
76. Ika Devi Ana, Shigeki Matsuya, Mihio Ohta, Kunio Ishikawa, Effects of added bioactive glass on the setting and mechanical properties of resin-modified glass ionomer cement., Biomaterials, 10.1016/S0142-9612(03)00151-0, 24, 18, 3061-3067, 24(18), 3061-3067, 2003.01.
77. Makoto Oda, Toshio Kaku, Atsushi Ookubo, Shigeki Matsuya, Kunio Ishikawa, The effects of hardening enviroment on the conversion to apatite and the mechanical strength of apatite cement., Journal of Japanese Society of Oral Implantology, 16(3), 400-408, 2003.01.
78. Yasuhiro Torii, Kousuke Itou, Yoshihiro Nishitani, Masahiro Yoshiyama, Kunio Ishikawa, Kazuomi Suzuki, Effect of self-setting primer containing N-acryloyl asparatic acid on enamel adhesion., Dental Materials, 19(4), 253-258, 2003.01.
79. Satoru Tanaka, Tamisuke Kishi, Ryoji Shimogoryo, Shigeki Matsuya, Kunio Ishikawa, Biopex® acquires anti-washout properties by adding sodium alginate into its liquid phase., Dental Materials Journal, 22, 3, 301-312, 22(3), 301-312, 2003.01.
80. Kunio Ishikawa, Youji Miyamoto, Taketomo Toh, Tetsuya Yuasa, Atsuo Ito, Masaru Nagayama, Kazuomi Suzuki, Fabrication of Zn containing apatite cement and its initial evaluation using human osteoblastic cells., Biomaterials, 23(2), 423-428, 2002.01.
81. Masaaki Takechi, Youji Miyamoto, Yukihiro Momota, Tetsuya Yuasa, Seikou Tatehara, Masaru Nagayama, Kunio Ishikawa, Kazuomi Suzuki, The in vitro antibiotic release from anti-washout apatite cement using chitosan., Journal of Materials Science: Materials in Medicine, 13(10), 973 ? 978, 2002.01.
82. Satoshi Nakabo, Yasuhiro Torii, Toshiyuki Itota, Kunio Ishikawa, Kazuomi Suzuki, Regulation of NaF release from bis-GMA/TEGDMA resin using γ-methacryloxypropyltrimethoxysilane, Dental Materials, 18, 81-87, 2002.01.
83. Satoshi Nakabo, Yasuhiro Torii, Toshiyuki Itota, Masahiro Yoshiyama, Kunio Ishikawa, Kazuomi Suzuki, Regulation of fluoride ion release from Na2SiF6 contained in resin based on hydrophobic siloxane layer coating., Journal of Oral Rehabilitation, 29 (7), 675-681, 2002.01.
84. Takamitsu Mano, Yoshiya Ueyama, Kunio Ishikawa, Kazuomi Suzuki, Tomohiro Matsumura, Initial tissue response to a titanium implant coated with apatite at room temperature using a blast coating method, Biomaterials, 23(9), 1931-1936, 2002.01.
85. Toshiyuki Suge, Kunio Ishikawa, Akiko Kawasaki, Satoru Imazato, Yuichiro Noiri, Kazuomi Suzuki, Takashi Matsuo, Shigeyuki Ebisu, Calcium phosphate precipitation method for the treatment of dentin hypersensitivity., American Journal of Dentistry, 15, 4, 220-226, 15(4), 220-226, 2002.01.
86. Yasuhiro Torii, Kousuke Itou, Yoshihiro Nishitani, Kunio Ishikawa, Kazuomi Suzuki, Effect of phosphoric acid etching prior to self-etching primer application on adhesion of resin composite to enamel and dentin., American Journal of Dentistry, 15(5): 305-308, 2002.01.
87. Yoshiya Ueyama, Kunio Ishikawa, Takamitsu Mano, Takahiro Koyama, Hitoshi Nagatsuka, Kazuomi Suzuki, Kazuo Ryoke, Usefulness as guided bone regeneration membrane of the alginate membrane., Biomaterials, 23(9), 2027-2033, 2002.01.
88. Yukihiro Momota, Youji Miyamoto, Kunio Ishikawa, Masaaki Takechi, Tetsuya Yuasa, Seiko Tatehara, Masaru Nagayama, Kazuomi Suzuki, Evaluation of feasibility of hydroxyapatite putty as a local hemostatic agent for bone., Journal of Biomedical Materials Research: Applied biomaterials, 10.1002/jbm.10332, 63, 5, 542-547, 63(5), 542 ? 547, 2002.01.
89. Atsuo Ito, Haruo Kawamura, Makoto Otsuka, Masako Ikeuchi, Hajime Ohgushi, Kunio Ishikawa, Kazuo Onuma, Nariko Kanzaki, Yu Sogo, Noboru Ichinose, Zinc-releasing calcium phosphate for stimulating bone formation., Materials Science and Engineering C, 10.1016/S0928-4931(02)00108-X, 22, 1, 21-25, 22(1),1-25, 2002.01.
90. Satoshi Nakabo, Yasuhiro Torii, Toshiyuki Itota, Kunio Ishikawa, Koji Miyazaki, Kazuomi Suzuki, Masahiro Yoshiyama, Effects of fluoride release from bis-GMA/TEGDMA resin regulated by γ-methacryloxypropyltrimethoxysilane on demineralization of bovine enamel., Biomaterials, 10.1016/S0142-9612(02)00079-0, 23, 16, 3503-3508, 23(16), 3503-3508, 2002.01.
91. Akiko Kawasaki, Kunio Ishikawa, Toshiyuki Suge, Hirotoshi Simizu, Kazuomi Suzuki, Takashi Matsuo, Shigeyuki Ebisu, Effects of plaque control on the occlusion of dentin tubule in situ., Journal of Oral Rehabilitation, 28(5), 439-449, 2001.01.
92. Kousuke Itou, Yasuhiro Torii, Takehiko Takimura, Kana Chikami, Kunio Ishikawa, Kazuomi Suzuki, Effect of priming time on tensile bond strength to bovine tooth and morphological structure of interfaces created by self-etching primers, The International Journal of Prosthodontics, 14, 225-230, 2001.01.
93. Masaaki Takechi, Kunio Ishikawa, Youji Miyamoto, Masaru Nagayama, Kazuomi Suzuki, Tissue responses to anti-washout apatite cement using chitosan when implanted in the rat tibia., Journal of Materials Science: Materials in Medicine, 12(7), 597-602, 2001.01.
94. Satoshi Nakabo, Yasuhiro Torii, Toshiyuki Itota, Masahiro Yoshiyama, Kunio Ishikawa, Kazuomi Suzuki, Effects of polysiloxane coating of NaF on the release profile of fluoride ion from bis-GMA/TEGDMA resin containing NaF, Dental Materilas Journal, 20(1), 53-62, 2001.01.
95. Tetsuya Yuasa, Youji Miyamoto, Kunio Ishikawa, Masaaki Takechi, Masaru Nagayama, Kazuomi Suzuki, In vitro resorption of three apatite cements with osteoclasts., Journal of Biomedical Materials Research, 54(3), 344-350, 2001.01.
96. Yoshiya Ueyama, Kunio Ishikawa, Takamitsu Mano, Takahiro Koyama, Tomohiro Matsumura, Kazuomi Suzuki, Initial tissue response to anti-washout apatite cement in the rat palatal region: Comparison with conventional apatite cement., Journal of Biomedical Materials Research, 55(4), 652-660, 2001.01.
97. Youji Miyamoto, Taketomo Toh, Kunio Ishikawa, Tetsuya Yuasa, Masaru Nagayama, Kazuomi Suzuki, Effect of added NaHCO3 on the basic properties of apatite cement., Journal of Biomedical Materials Research, 54(3), 311-319, 2001.01.
98. Elisete Kazumi Watanabe, Hirofumi Yatani, Kunio Ishikawa, Kazuomi Suzuki, Atsushi Yamashita, Pilot study of conditioner/primer effects on resin-dentin bonding after provisional cement contamination using SEM, energy dispersive x-ray spectroscopy, and bond strength evaluation measures., The Journal of Prosthetic Dentistry, 83(3), 349-355, 2000.01.
99. Guoqiang Guan, Teruko Takano-Yamamoto, Manabu Miyamoto, Tetsuo Hattori, Kunio Ishikawa, Kazuomi Suzuki, Shear bond strengths of orthodontic plastic brackets., American Journal of Orthodontics and Dentofacial Orthopedics, 117(4), 438-443, 2000.01.
100. Kazuomi Suzuki, Kunio Ishikawa, Katsumi Sugiyama, Hiroaki Furuta, Fumio Nishimura, Content and release of bisphenol A from polycarbonate dental products., Dental Materials Journal, 19(4), 389-395, 2000.01.
101. Kazuomi Suzuki, Kunio Ishikawa, Yoshihiro Nishitani, Kousuke Itou, Yasuhiro Torii, Norihiko Nishiyama, Kimiya Nemoto, Adhesion of restrative resin to teeth: Effects of functional groups of dentin primer to collagen, Dentistry in Japan, 36, 93-97, 2000.01.
102. Kousuke Itou, Yasuhiro Torii, Yoshihiro Nishitani, Kunio Ishikawa, Kazuomi Suzuki, Kiyoshi Inoue, Effect of self-etching primers containing N-acryloyl aspartic acid on dentin adhesion., Journal of Biomedical Materials Research, 51(4), 569-574, 2000.01.
103. Teruo Kasai, Kunio Ishikawa, Kazuomi Suzuki, Hirofumi Yatani, Initial evaluation of a ceramic form as a reconstructive material for bone defect., Dental Materials Journal, 19(4), 381-388, 2000.01.
104. Elisete Kazumi Watanabe, Hirofumi Yatani, Atsushi Yamashita, Kunio Ishikawa, Kazuomi Suzuki, Effects of thermocycling on the tensile bond strength between resin cement and dentin surfaces after temporary cement application, International Journal of Prosthodontics, 12(3), 230-235, 1999.01.
105. Jiro Tanaka, Kunio Ishikawa, Hirofumi Yatani, Atsushi Yamashita, Kazuomi Suzuki, Correlation of dentin bond durability with water absorption of bonding layer, Dental Materials Journal, 18(1), 11-18, 1999.01.
106. Jiro Tanaka, Kunio Ishikawa, Hirofumi Yatani, Atsushi Yamashita, Kazuomi Suzuki, Effect of carboxyl group in O-methacryloyl-N-acyl tyrosines on adhesiveness to unetched human dentin, Dental Materials Journal, 18(1), 87-95, 1999.01.
107. Kunio Ishikawa, Shozo Takagi, Laurence C Chow, Kazuomi Suzuki, Reaction of calcium phosphate cements with different amounts of tetracalcium phosphate and dicalcium phosphate anhydrous., Journal of Biomedical Materials Research, 46(4), 504-510, 1999.01.
108. Kunio Ishikawa, Yoshiya Ueyama, Takamitsu Mano, Takahiro Koyama, Kazuomi Suzuki, Tomohiro Matsumura, Self-setting barrier membrane for guided tissue regeneration method: Initial evaluation of alginate membrane made with sodium alginate and calcium chloride aqueous solutions., Journal of Biomedical Materials Research, 47(2), 111-115, 1999.01.
109. Kunio Ishikawa, Youji Miyamoto, Masaaki Takechi, Yoshiya Ueyama, Kazuomi Suzuki, Masaru Nagayama, Tomohiro Matsumura, Effects of neutral sodium hydrogen phosphate on setting reaction and mechanical strength of hydroxyapatite putty, Journal of Biomedical Materials Research, 44(3), 322-329, 1999.01.
110. Toshiyuki Suge, Kunio Ishikawa, Akiko Kawasaki, Kazuomi Suzuki, Takashi Matsuo, Shigeyuki Ebisu, Evaluation of post-treatment solutions for clinical use with the calcium phosphate precipitation method., Journal of Dentistry, 27, 487-496, 1999.01.
111. Youji Miyamoto, Kunio Ishikawa, Masaaki Takechi, Taketomo Toh, Tetsuya Yuasa, Masaru Nagayama, Kazuomi Suzuki, Histological and compositional evaluations of three types of calcium phosphate cements when implanted in subcutaneous tissue immediately after mixing. Journal of Biomedical Materials, Research: Applied Biomaterials, 48(1), 36-42, 1999.01.
112. Elisete Kazumi Watanabe, Atsushi Yamashita, Hirofumi Yatani, Kunio Ishikawa, Kazuomi Suzuki, Improvement in the tensile bond strength between resin cement and dentin surfaces after temporary cement application., The International Journal of Prosthodontics, 11(3), 203-211, 1998.01.
113. Jiro Tanaka, Kunio Ishikawa, Hirofumi Yatani, Atsushi Yamashita, Kazuomi Suzuki, Steric effects of N-acryl group in O-methacryloyl-N-acyl tyrosines on the adhesiveness of unetched human dentin, Dental Materials Journal, 17(4), 233-238, 1998.01.
114. Masaaki Takechi, Youji Miyamoto, Kunio Ishikawa, Masaru Nagayama, Masayuki Kon, Kenzo Asaoka, Kazuomi Suzuki:, Effects of added antibiotics on the basic properties of anti-washout-type fast-setting calcium phosphate cement., Journal of Biomedical Materials Research, 39(2), 308-316, 1998.01.
115. Masaaki Takechi, Youji Miyamoto, Kunio Ishikawa, Taketomo Toh, Tetsuya Yuasa, Masaru Nagayama, Kazuomi Suzuki, Initial histological evaluation of anti-washout type fast-setting calcium phosphate cement following subcutaneous implantation, Biomaterials, 19(22), 2057-2063, 1998.01.
116. Masayuki Kon, Youji Miyamoto, Kenzo Asaoka, Kunio Ishikawa, Hae-Hyong Lee, Development of calcium phosphate cement for rapid crystallization to apatite, Dental Materials Journal, 17(4), 223-232, 1998.01.
117. Shozo Takagi, Laurence C Chow, Kunio Ishikawa, Formation of hydroxyapatite in new calcium phosphate cements, Biomaterials, 19(17), 1593-1599, 1998.01.
118. Youji Miyamoto, Kunio Ishikawa, Masaaki Takechi, Taketomo Toh, Tetsuya Yuasa, Masaru Nagayama, Kazuomi Suzuki, Basic properties of calcium phosphate cement containing atelocollagen in its liquid or powder phases., Biomaterials, 19(8), 707-715, 1998.01.
119. Ishikawa Kunio, Tsuru Kanji, Ichiro Takahashi, Effects of humidity on calcite block fabrication using calcium hydroxide compact., Ceramics International.
120. Ishikawa Kunio, Effects of low crystalline carbonate apatite on proliferation and osteoblastic differentiation of human bone marrow cells., Journal of Materials Science: Materials in Medicine.
121. Ishikawa Kunio, Tsuru Kanji, Toita Riki, Fabrication of porous calcite using chopped nylon fiber and its evaluation using rats., Journal of Materials Science: Materials in Medicine.
122. Ishikawa Kunio, Tsuru Kanji, Toita Riki, Fabrication of porous calcite using chopped nylon fiber and its evaluation using rats., Journal of Materials Science: Materials in Medicine.
123. Ishikawa Kunio, Effect of calcium-ozone treatment on chemical and biological properties of polyethylene terephthalate. , Journal of Biomedical Materials Research.
124. Ishikawa Kunio, Tsuru Kanji, Effects of PLGA reinforcement methods on the mechanical property of carbonate apatite foam., Bio-Medical Materials and Engineering, in press..