, Drosophila transglutaminase (dTG) regulates transcriptional activity by incorporating polyamines into the NF-kB-like transcription factor Relish
||Shibata, T., Maki, K., Fujikawa, T., and Kawabata, S., Transglutaminase-catalyzed crosslinking of peritrophic matrix proteins maintains the gut epithelial immunity in Drosophila. , Entomology 2017 in the Annual meeting of Entomological Society of America., 2017.11.
||hibata, T., Maki, K., Fujikawa, T., and Kawabata, S., Transglutaminase-catalyzed crosslinking of peritrophic matrix proteins maintains the gut epithelial immunity in Drosophila., European Drosophila Research Conference, 2017.09.
||Shun-ichiro Kawabata, Transglutaminase-catalyzed relish crosslinking suppresses innate immune signaling in the Drosophila., Gordon Research Conference on Transglutaminase in Human Disease Processes, 2014.06.
||Shun-ichiro Kawabata, Transglutaminase-Catalyzed Protein-Protein Crosslinking Maintains the Gut Epithelial Immunity in Drosophila., The 55th Annual Drosophila Research Conference, 2014.03.
||Shun-ichiro Kawabata, Transglutaminase-catalyzed crosslinking suppresses the activity of the NF-κB-like transcription factor relish in Drosophila., The First Asian Invertebrate Immunology Symposium, 2014.02.
||Shun-ichiro Kawabata, Transglutaminase-catalyzed protein crosslinking suppresses innate immune signaling in the Drosophila gut, 国際エンドトキシン自然免疫学会, 2012.09, Mammalian transglutaminases (TGs) play important roles in numerous physiological phenomena such as blood coagulation and skin formation via protein-protein crosslinking. Recently, we reported that the RNAi of the TG gene in Drosophila causes a pupal semi-lethal phenotype and abnormal morphology, including abnormal wing formation and abdominal melanization (PLoS ONE, 2010). TG-RNAi flies had a shorter life span than their wild-type counterparts, and approximately 90% of flies died within 30 days after eclosion under conventionally reared conditions. We show that Drosophila cytoplasmic TG suppresses innate immune signaling in the gut. TG-RNA-mediated interference (TG-RNAi) caused a short life span under non-sterile conventionally reared conditions, but not under germ-free conditions. Under conventionally reared conditions, TG-RNAi enhanced the expression of immune deficiency (IMD) pathway-controlled antimicrobial peptide genes. Ingestion of gut lysates prepared from conventionally reared TG-RNAi flies into non-TG-RNAi flies resulted in the short life span of the recipients. TG-RNAi under conventionally reared conditions triggered severe apoptosis in the gut and induced the translocation of Relish, the NF-κB-like transcription factor of the IMD pathway, from the cytoplasm to the nucleus. Ingestion of synthetic amine donors by non-TG-RNAi flies, which inhibits the TG-catalyzed protein-protein crosslinking reaction, also induced the nuclear translocation of Relish and enhanced the expression of IMD-controlled antimicrobial peptide genes in the gut. We conclude that TG-catalyzed Relish crosslinking suppresses the IMD-signaling pathway to maintain a buffered threshold required for immune tolerance against commensal microbes..
||Shun-ichiro Kawabata, Pattern recognition for β-1,3-D-glucans by factor G is accomplished via a carbohydrate-binding cleft that is evolutionally conserved between horseshoe crab and bacteria., STINT, 2010.03.
||Shun-ichiro Kawabata , Horseshoe crab factor G utilizes a carbohydrate-binding cleft that is conserved among invertebrates and bacteria for the recognition of β-1,3-glucans. , International Society of Developmental and Comparative Immunology, 2009.07.
||Shun-ichiro Kawabata, A lipopolysaccharide-responsive C3 convertase in horseshoe crab complement activation, Jacques-Monod conference, 2009.05.
||Shun-ichiro Kawabata, A structural perspective on the interaction between lipopolysaccharide and horseshoe crab factor C, Regulation in Innate Immunity, 2008.10.
||Shun-ichiro Kawabata, Amplification of the horseshoe crab innate immune reaction by an antimicrobial peptide, 5th World Fisheries Congress, 2008.10.
||Shun-ichiro Kawabata, Chitin-binding proteins involved in the horseshoe crab innate immunity, Jacques-Monod Conference, 2006.06.
||Shun-ichiro Kawabata, A serine protease zymogen on the horseshoe crab hemocyte functions as a pattern-recognition protein for lipopolysaccharides., The 16th Naito Conference on Innate Immunity in Medicine and Biology [I]., 2003.10.
||Shun-ichiro Kawabata, Molecular basis of non-self recognition by horseshoe crab lectins. NEC soft-Kyushu University Seminar. The front-line of protein structure-function studies, NEC soft-Kyushu University Seminar, 2003.10.
||Shun-ichiro Kawabata, Molecular basis of the horseshoe crab innate immunity., 2003.05.