Kyushu University Academic Staff Educational and Research Activities Database
List of Papers
kazunori Nakagawa Last modified date:2019.12.20

Lecturer / Pathobiology / Department of Basic Medicine / Faculty of Medical Sciences

1. Kazunori Nakagawa, Yutaka Nakashima, Pathologic intimal thickening in human atherosclerosis is formed by extracellular accumulation of plasma-derived lipids and dispersion of intimal smooth muscle cells, Atherosclerosis, 10.1016/j.atherosclerosis.2018.03.039, 274, 235-242, 2018.07, Background and aims: Pathologic intimal thickening (PIT) is an important stage of atherosclerosis that leads to atheroma. The present study aimed to clarify the pathogenesis of PIT in humans. Methods: Coronary arteries were obtained from 43 autopsy subjects aged 15–49 years. Non-atherosclerotic intima and atherosclerotic intimal lesions were classified into four groups, i.e. diffuse intimal thickening, fatty infiltration, fatty streak, and PIT, and the number and density of macrophages and smooth muscle cells (SMCs) were determined. Components of the lesions and proliferative and apoptotic activities of macrophages and SMCs were investigated by immunohistochemistry and TUNEL assay. Results: Extracellular lipids accumulated mildly in the fatty infiltration and fatty streak, and abundantly in the PIT to form the lipid pool. The extracellular lipids co-localized with apolipoprotein B and fibrinogen. Macrophage foam cells accumulated in the fatty streak and PIT, but no TUNEL-positive macrophages were detected in any lesion. No significant difference in the number of SMCs was found between the four groups, but the density of SMCs decreased in the fatty streak and PIT. The decrease correlated with an increase in the number of macrophages, and the accumulation of extracellular lipids in the lipid pool. Neither Ki-67-positive nor TUNEL-positive SMCs were detected in any lesion. Conclusions: In PIT in human atherosclerosis, the lipid pool is formed by infiltration and deposition of plasma-derived lipids. Intimal SMCs are dispersed in association with macrophage infiltration and lipid pool formation without undergoing significant proliferation or death..
2. Tsukube T, agi N, Hoshino M, Nakashima Y, Nakagawa K, Okita Y, Impact of synchrotron radiation-based X-ray phase-contrast tomography on understanding various cardiovascular surgical pathologies., Gen Thorac Cardiovasc Surg, 10.1007/s11748-015-0565-4, [Epub ahead of print], 2015.09, At SPring-8, synchrotron radiation-based X-ray phase-contrast tomography (PCXI) has been developed to measure the inner structures of biological soft tissue without destroying them. To resolve the three-dimensional (3D) morphology, we have applied PCXI to various cardiovascular tissue samples, including the thoracic aorta, ductus arteriosus, and cardiac conduction system. In the aortic walls, PCXI demonstrated differences in 3D structures of tunica media of aortic dissection. These findings correlated well with the irregularity of the structure of the media. In the surgically excised sample of coarctation of the aorta, PCXI showed 3D morphological changes in transition from the ductus arteriosus to the descending aorta. PCXI is also useful for examining abnormalities of the cardiac conduction system in congenital heart defects. Synchrotron radiation-based X-ray phase-contrast tomography has strong modality for analyzing 3D morphology and is useful for understanding the pathophysiology of various cardiovascular surgical pathologies..
3. Onimaru M, Yonemitsu Y, Fujii T, Tanii M, Nakano T, Nakagawa K, Kohno RI, Hasegawa M, Nishikawa SI, Sueishi K., VEGF-C regulates lymphangiogenesis and capillary stability by regulation of PDGF-B., Am J Physiol Heart Circ Physiol., [Epub ahead of print], 2009.09.
4. Murakami Y, Ikeda Y, Yonemitsu Y, Onimaru M, Nakagawa K, Kohno R, Miyazaki M, Hisatomi T, Nakamura M, Yabe T, Hasegawa M, Ishibashi T, Sueishi K., Inhibition of nuclear translocation of apoptosis-inducing factor is an essential mechanism of the neuroprotective activity of pigment epithelium-derived factor in a rat model of retinal degeneration., Am J Pathol., 173(5):1326-38., 2008.11.
5. Ikeda Y, Yonemitsu Y, Onimaru M, Nakano T, Miyazaki M, Kohno RI, Nakagawa K, Ueno A, Sueishi K, Ishibashi T., The regulation of vascular endothelial growth factors (VEGF-A, -C, and -D) expression in the retinal pigment epithelium., Exp Eye Res., 83(5):1031-1040.
, 2006.05.
6. Nagata S, Okano S, Yonemitsu Y, Yoshida K, Nagata H, Nakagawa K, Tomita Y, Yoshikai Y, Shimada M, Maehara Y, Sueishi K., The critical roles of memory T cells and anti-donor immunoglobulin in rejection of allogeneic bone marrow cells in sensitized recipient mice., Transplantation, 82(5):689-98., 2006.05.
7. Kuroda J, Nakagawa K, Yamasaki T, Nakamura K, Takeya R, Kuribayashi F, Imajoh-Ohmi S, Igarashi K, Shibata Y, Sueishi K, Sumimoto H., The superoxide-producing NAD(P)H oxidase Nox4 in the nucleus of human vascular endothelial cells., Genes to Cells, 10.1111/j.1365-2443.2005.00907.x, 10, 12, 1139-1151, 10(12):1139-51, 2005.12.
8. Shikada Y, Yonemitsu Y, Koga T, Onimaru M, Okano S, Sata S, Nakagawa K, Yoshino I, Maehara Y, Sueishi K., PDGF-AA is an essential and autocrine regulator of VEGF expression in non-small cell lung carcinomas., Cancer Research, 65(16):7241-8, 2005.01.
9. Sassa Y, Hata Y, Murata T, Yamanaka I, Honda M, Hisatomi T, Fujisawa K, Sakamoto T, Kubota T, Nakagawa K, Sueishi K, Ishibashi T., Functional role of Egr-1 mediating VEGF-induced tissue factor expression in the retinal capillary endothelium., Graefes Arch Clin Exp Ophthalmol, 10.1007/s00417-002-0576-6, 240, 12, 1003-1010, 240(12):1003-10, 2002.01.
10. Yamashita A, Yonemitsu Y, Okano S, Nakagawa K, Nakashima Y, Irisa T, Iwamoto Y, Nagai Y, Hasegawa M, Sueishi K, Fibroblast growth factor-2 determines severity of joint disease in adjuvant-induced arthritis in rats., J Immunol, 168, 1, 450-457, 168(1),pp450-457, 2002.01.
11. Masaki I, Yonemitsu Y, Komori K, Ueno H, Nakashima Y, Nakagawa K, Fukumura M, Kato A, Hasan M, Nagai Y, Sugimachi K, Alton EWFW, Hasegawa M, Sueishi K., Recombinant Sendai virus-mediated gene transfer to vasculature: a new class of efficient gene transfer vector to the vascular system., FASEB J, 15, 7, 1294-1296, 15(7): 1294-1296, 2001, 2001.01.
12. Ishibashi H, Shiratuchi T, Nakagawa K, Onimaru M, Sugiura T, Sueishi K, Shirasuna K, Hypoxia-induced angiogenesis of cultured human salivary gland carcinoma cells enhances vascular endothelial growth factor production and basic fibroblast growth factor release, Oral Oncology, 10.1016/S1368-8375(00)00062-2, 37, 1, 77-83, 37(1): 77-83, 2001.01.