Kyushu University Academic Staff Educational and Research Activities Database
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Masahiro Oike Last modified date:2019.05.27

Associate Professor / Science for Biological Information
Department of Basic Medicine
Faculty of Medical Sciences


Graduate School
Undergraduate School
Administration Post
Other


E-Mail
Homepage
http://hyoka.ofc.kyushu-u.ac.jp/search/details/K000908/index.html
Phone
092-642-6076
Fax
092-642-6079
Academic Degree
PhD
Country of degree conferring institution (Overseas)
No
Field of Specialization
Pharmacology
Total Priod of education and research career in the foreign country
02years00months
Outline Activities
Research:
(1) Vascular endothelium: My main research interest is endothelial signal transduction. I have clarified mechanosensitive endothelial responses, focusing on ATP release mechanisms. Furthermore, I have examined the effects of various hazardous environments, such as hypoxia, glucose overload and hyperlipidemia, on endothelial functions.
(2) I have developed an in vitro reconstruction system of smooth muscle contraction, using cultured smooth muscle cells, and examined the detailed contractile mechanisms of vascular and airway smooth muscle cells. Especially I have established the in vitro model system of airway hyperresponsiveness and revealed the possible involvement of RhoA and MMP-1 in hyperresponsiveness.
(3) in silico drug discovery: I have been using in sillico screening for discovering anti-cancer metastatic drugs.
(4) I have developed in silico drug repositioning system and have been conducting experiments to prove the usefulness of this system.

Education:
I give lectures of introduction to pharmacology, autonomic pharmacology, CNS pharmacology, endocrine pharmacology, pulmonary pharmacology and gastrointestinal pharmacology to undergraduate medical students (3rd grade), and conduct students' practice of autonomic nerve drugs and cardiovascular drugs. I use in silico simulation programs in students' practice as an alternative of in vivo experiments.

Social activities:
I have been involved in the 'Ground Research Announcement for Space Utilization" promoted by NASDA , JAXA and Japan Space Forum.
Research
Research Interests
  • Development of peptide drugs targeting protein-protein interface.
    keyword : protein-protein interface, in silico drug development
    2018.04~2022.03.
  • Drug repositioning using in silico screening.
    keyword : in silico screening, drug repositioning
    2016.04.
  • Prediction of drug-target proteins using computational chemistry.
    keyword : computational chemistry, drug target, protein, in silico screening
    2015.10~2018.03.
  • Drug discovery using in silico screening.
    keyword : in silico screening, cancer metastasis, TGFβ
    2013.04.
  • Interaction between vascular endothelial cells and tumor cells.
    keyword : endothelial cells, tumor cells, protein
    2008.09~2014.12Angiogenesis is the proliferation and migration of vascular endothelial cells, and plays a key role in tumor growth and wound healing. We have found that supernatant of a cirtain cell contains protein(s) that markedly suppress the proliferation of endothelial cells. This study aims to identify this protein..
  • Exploration of endogenous anti-angiogenic substance.
    keyword : angiogenesis, endothelial cells, anti-angiogenic factor, protein
    2007.09Angiogenesis is the proliferation and migration of vascular endothelial cells, and plays a key role in tumor growth and wound healing. We have found that supernatant of a cirtain cell contains protein(s) that markedly suppress the proliferation of endothelial cells. This study aims to identify this protein..
  • Possible role of MMP-1 in the Th2 cytokines-induced development of airway hyperresponsiveness.
    keyword : Th2 cytokines, MMP-1, airway hyperresponsiveness, collagen
    2005.04~2012.12Possible role of MMP-1 in the Th2 cytokines-induced development of airway hyperresponsiveness..
  • Identification of endogenous smooth muscle organization factor.
    keyword : vascular smooth muscle cells, intercellular information
    2005.04~2015.03I am clarifying the possible invovelment of endothelium-derived factor(s) in the arrangement of vascular smooth muscle cells into vessel-like structure..
  • Molecular mechanisms of endothelial mechanosensitivity.
    keyword : vascular endothelial cells, small G protein, ATP, Dbl protein, integrin
    1998.04~2014.04Molecular mechanisms of endothelial mechanosensitivity. I have examined how vascular endothelium converts physical forces into chemical signals and how these signals ultimately lead to endothelial functions including ATP release..
  • Molecular mechanisms of airway hyperresponsiveness and exploration of anti-hyperresponsive drugs.
    keyword : airway smooth muscle cells, airway hyperresponsiveness, theophylline, cAMP, small G protein.
    2000.04~2006.12Cellular mechanisms of airway hyperresponsiveness and search for drugs that ameliorate it. I have developed a novel in vitro method to simulate airway hyperresponsiveness, using cultured airway smooth muscle cells. I have found that theophylline reverses airway hyperresponsiveness by inhibiting small G protein Rho with cAMP. I am now examining other possibilities to efficiently ameliorate hyperresponsiveness..
  • ATP release pathway in vascular endothleial cells.
    keyword : vascular endothelial cells, ATP release, chloride channels
    1996.04~2002.12ATP release pathway in vascular endothleial cells. ATP is released from vascular endothelium in response to mechanical stress. I have clarified that mechanosensitive ATP release is obtained through volume-regulated anion channels..
Current and Past Project
  • This study aims to develop an efficient method for cell culture in space station.
  • The purpose of this poject was to clarify the effects of gravity change on endothelial functions and their molecular emchanisms.
Academic Activities
Books
1. Biology of airway smooth muscles..
2. Oike M, Kimura C, Ito Y, Histamine Research in the New Millenium. "Glucose overload attenuates histamine H2 receptor-mediated Ca2+ mobilization due to protein kinase C production in bovine cerebral endothelial cells.", Elsevier, 415-416, 2001.01.
Reports
1. Computer simulation as an alternative to animal use in pharmacology education .
2. ATP release pathway in vascular endothelial cells.
3. Mechanism of nitric oxide production in vascular endothelium and the significance as therapeutic target--special reference to the relationship with superoxides.
Papers
1. Chiwaka Kimura, Masayuki Hayashi, Yuri Mizuno, Masahiro Oike, Endothelium-dependent epithelial-mesenchymal transition of tumor cells: Exclusive roles of transforming growth factor β1 and β2., Biochim Biophys Acta, 1830, 10, 4470-4481, 2013.10.
2. Kenji Miki, Hiromitsu Tanaka, Yoko Nagai, Chiwaka Kimura, Masahiro Oike, Transforming growth factor β1 alters calcium mobilizing properties and endogenous ATP release in A549 cells: possible implications for cell migration., Journal of Pharmacological Sciences, 113, 4, 113, 4, 387-394, 2010.08.
3. Ohta Y, Hayashi M, Kanemaru T, Abe K, Ito Y, Oike M., Dual modulation of airway smooth muscle contraction by Th2 cytokines via matrix metalloproteinase-1 production. , Journal of Immunology, 180(6):4191-4199, 2008.03.
4. Kimura C, Oike M, Watanabe M, and Ito Y, Proapoptotic nitric oxide production in amyloid beta protein-treated cerebral microvascular endothelial cells, Microcirculation, 14(2):89-97, 2007.02.
5. Hirakawa M, Oike M, Watanabe M, Karashima Y, Ito Y, Pivotal role of integrin alpha5beta1 in hypotonic stress-induced responses of human endothelium., FASEB Journal, 20(12):1992-1999, 2006.12.
6. Hirakawa M, Oike M, Karashima Y, Ito Y, Sequential activation of RhoA and FAK/paxillin leads to ATP release and actin reorganization in human endothelium, Journal of Physiology, 10.1113/j.physiol.2004.065334, 558, 2, 479-488, 558(Pt2):479-488, 2004.07.
7. Kimura C, Oike M, Ohnaka K, Nose Y, Ito Y., Constitutive nitric oxide production in bovine aortic and brain microvascular endothelial cells: a comparative study., Journal of Physiology, 10.1113/jphysiol.2003.057059, 554, 3, 721-730, 554(Pt3):721-30., 2004.02.
8. Sakai J, Oike M, Hirakawa M, Ito Y., Theophylline and cAMP inhibit lysophosphatidic acid-induced hyperresponsiveness of bovine tracheal smooth muscle cells., Journal of Physiology, 10.1113/jphysiol.2003.039024, 549, 1, 171-180, 549(Pt1):171-80, 2003.05.
9. Hisadome K, Koyama T, Kimura C, Droogmans G, Ito Y, Oike M., Volume-regulated anion channels serve as an auto/paracrine nucleotide release pathway in aortic endothelial cells., Journal of General Physiology, 10.1085/jgp.20028540, 119, 6, 511-520, 119(6):511-20, 2002.07.
10. Hirakawa M, Oike M, Masuda K, Ito Y., Tumor cell apoptosis by irradiation-induced nitric oxide production in vascular endothelium., Cancer Research, 62, 5, 1450-1457, 62(5):1450-7, 2002.03.
11. Koyama T, Oike M, Ito Y., Involvement of Rho-kinase and tyrosine kinase in hypotonic stress-induced ATP release in bovine aortic endothelial cells., Journal of Physiology, 10.1111/j.1469-7793.2001.0759e.x, 532, 3, 759-769, 532(Pt 3):759-69., 2001.05.
12. Kimura C, Oike M, Koyama T, Ito Y., Impairment of endothelial nitric oxide production by acute glucose overload., American Journal of Physiology, 280, 1, E171-E178, 280(1):E171-8., 2001.01.
13. Oike M, Kimura C, Koyama T, Yoshikawa M, Ito Y., Hypotonic stress-induced dual Ca2+ responses in bovine aortic endothelial cells., American Journal of Physiology, 279, 2, H630-H638, 279(2):H630-8., 2000.08.
14. Kimura C, Oike M, Ito Y., Acute glucose overload abolishes Ca2+ oscillation in cultured endothelial cells from bovine aorta: a possible role of superoxide anion., Circulation Research, 82, 6, 677-685, 82(6):677-85., 1998.04.
Presentations
1. Significance of educational use of drug development program suite myPresto for medical students. .
2. In silico screening and development of small molecule compounds for novel TGFβ1 inhibitors..
3. Constitutional release of TGFβs from vascular and lymphatic endothelial cells and its possible contribution to epithelial-mesenchymal transition of tumor cells..
4. Computer-based alternative to the use of experimental animals in pharmacological education., [URL].
Membership in Academic Society
  • The Japanese Pharmacological Society
  • The Chem-Bio Informatics Society
Educational
Educational Activities
Postgraduates (graduate school of medical sciences): I have supervised nine postgraduate students, one research students and three researchers from China and Korea. Eight students have been awarded Ph.D. or M.Sc. degree already.
Undergraduate class (faculty of medicine, department of medicine): I give lectures of pharmacology to undergeraduate medical students (14 hours), and conduct practice of pharmacology (10 hours).
(faculty of medicine, department of biomedical science): I give lecture of biological response and regulation (1 hour).
Other Educational Activities
  • 2019.05, I introduced a use of computer simulation programs as an alternative to animal use in pharmacology education (Folia Pharmacologica Japonica. 144(2), 95-97, 2014). .
  • 2004.08.
  • 2004.06.
Social
Professional and Outreach Activities
There would be various changes in vascular functions in microgravity, and therefore clinical courses of cardiovascular diseases and malignancy of astronauts may be different from those on earth, especially in a long term. I have examined the effects of gravity on endothelial functions especially mechanosensitivity as research commissions with Japan Aerospace Exploration Agency..