メカノバイオマテリアル：細胞の機能・行動を操作する微視的細胞外力学場設計
キーワード：ナノバイオメカニクス、メカノバイオロジー、メカノバイオマテリアル、メカノタクシス
2001.06.
| 木戸秋 悟（きどあき さとる） | データ更新日：2024.04.20 |
主な研究テーマ
蛋白質バイオメカニクス：蛋白質吸着・蛋白質間相互作用を制御する力学・熱力学研究
キーワード：分子間相互作用力測定
1997.04.
キーワード：分子間相互作用力測定
1997.04.
マトリックス工学：電界紡糸法による組織工学人工マトリックス・骨格・デバイスの開発研究
キーワード：細胞操作材料
2002.06.
キーワード：細胞操作材料
2002.06.
DNA分子の高次構造制御および溶液・マトリックス中での移動・拡散過程の研究
キーワード：生体高分子直接観察、自己秩序分子集合体形成
1993.04.
キーワード：生体高分子直接観察、自己秩序分子集合体形成
1993.04.
従事しているプロジェクト研究
エントロピー増大に逆らう革新材料「力学極性ゲル」による物質・エネルギー・生物の整流化
2022.11～2028.03, 代表者：石田 康博, 理化学研究所, 科学技術振興機構.
2022.11～2028.03, 代表者：石田 康博, 理化学研究所, 科学技術振興機構.
AMED-CREST 『幹細胞の品質保持培養のためのメカノバイオマテリアルの開発』
2015.12～2021.03, 代表者：木戸秋 悟, 先導物質化学研究所, 国立研究開発法人日本医療研究開発機構（AMED）
.
2015.12～2021.03, 代表者：木戸秋 悟, 先導物質化学研究所, 国立研究開発法人日本医療研究開発機構（AMED）
.
幹細胞系統決定に関与する細胞内レドックスタンパク質の発現解析
2009.12.
2009.12.
力場ー化学場共役系における細胞運動制御
2010.08.
2010.08.
弾性基材表面への細胞接着力解析
2008.06.
2008.06.
弾性基材を用いた幹細胞プロテオミクス解析
2009.01～2012.09.
2009.01～2012.09.
附置研究所間アライアンスによる ナノとマクロをつなぐ物質・システム創製戦略プロジェクト (ナノマクロ物質・デバイス・システム創製アライアンス)：G3 医療・デバイスシステム
2010.04～2015.03, 大阪大学.
2010.04～2015.03, 大阪大学.
最先端研究開発支援プロジェクト 『一分子解析技術を基盤とした革新的ナノバイオデバイスの開発研究（川合最先端PJ）』
2010.04～2014.03, 代表者：川合 知二, 大阪大学.
2010.04～2014.03, 代表者：川合 知二, 大阪大学.
科学技術振興機構 さきがけ 『細胞運動・機能を操作するナノ・マイクロメカニカルシステムの構築 』
2009.10～2013.03, 代表者：木戸秋 悟, 九州大学.
2009.10～2013.03, 代表者：木戸秋 悟, 九州大学.
科学技術振興機構 戦略的基礎研究推進事業『ゲノムレベルの生体分子相互作用探索と医療に向けたナノレゴ開発』
2002.11～2008.03, 代表者：林崎 良英, 理化学研究所, 科学技術振興機構.
2002.11～2008.03, 代表者：林崎 良英, 理化学研究所, 科学技術振興機構.
科学技術振興機構 戦略的基礎研究推進事業 『自己生成するナノ秩序体：高次構造制御と機能発現』
1999.10～2004.03, 代表者：吉川研一, 京都大学, 科学技術振興機構.
1999.10～2004.03, 代表者：吉川研一, 京都大学, 科学技術振興機構.
研究業績
主要著書
主要原著論文
| 1. | H. Miyoshi*, M, Yamazaki, H. Fujie, S. Kidoaki, Guideline for design of substrate stiffness for mesenchymal stem cell culture based on heterogeneity of YAP and RUNX2 responses, Biophysics and Physicobiology, 2023.02. |
| 2. | S. Masaike, Y. Tsuji, and S.Kidoaki, Local pH mapping in the cell adhesion nano-interfaces on a pH-responsive fluorescence-dye-immobilized substrate, Anal. Sci., 39, 347–355 (2023), 2023.03. |
| 3. | H. Ebata and S.Kidoaki,, Interplay among cell migration, shaping, and traction force on a matrix with cell-scale stiffness heterogeneity, Biophysics and Physicobiology, Volume 19 Article ID: e190036 , 2022.12. |
| 4. | Sayaka Masaike, Saori Sasaki, Hiroyuki Ebata, Kosuke Moriyama, Satoru Kidoaki, Adhesive-ligand-independent cell-shaping controlled by the lateral deformability of a condensed polymer matrix, POLYMER JOURNAL, 10.1038/s41428-021-00577-w, 2021.11, Cell adhesion on biomaterial surfaces has been extensively studied from the perspective of the adsorption properties of adhesive ligands, while recent research on mechanobiology has been revealing a critical role of the mechanical properties of the extracellular milieu in the control of cell adhesion, such as the stiffness and viscoelasticity of the matrix. Although the effects of the lateral mobility of an adhesive ligand have been intensively investigated in a model substrate with water-soluble polymer layers, less is known about those in the setting of lateral deformability of hydrophobic condensed polymer layers. In this study, to help clarify this issue, we used PNIPAAm-grafted substrates with a well-controlled degree of graft-polymerization (DGP) as a typical hydrophobic condensed polymer surface at a cell culture temperature of 37 degrees C. We observed a clear negative correlation between cell spreading and DGP of PNIPAAm regardless of the amount of fibronectin adsorbed on the substrates, which was found to be attributable to the lateral deformability of a condensed PNIPAAm layer based on lateral force microscopic analysis. The surface-lateral-deformation-induced modulation in stability and maturation of focal adhesion of the cells is discussed in relation to the matrix-strain-induced alteration of the density distribution of adsorbed adhesive ligands.. |
| 5. | H. Ebata and S. Kidoaki, Avoiding tensional equilibrium in cells migrating on a matrix with cell-scale stiffness-heterogeneity, Biomaterials, 120860, 2021., 2021.05. |
| 6. | S. Masuda,T. Kuboki, S. Kidoaki, S-T. Lee, S. Ryuzaki, K. Okamoto, Y. Arima, K. Tamada, High axial and lateral resolution on self-assembled gold nanoparticle metasurfaces for live-cell imaging, ACS Appl. Nano Mater., 3, 11, 11135-11142, 2020.10. |
| 7. | M. Yamazaki, S. Kidoaki, H. Fujie, H. Miyoshi, Designing elastic modulus of cell culture substrate to regulate YAP and RUNX2 localization for controlling differentiation of human mesenchymal stem cells, Anal. Sci., in press, 2020.10. |
| 8. | T. Fukuyama, H. Ebata, Y. Kondo, S. Kidoaki, K. Aoki, Y. T. Maeda, Why epithelial cells collectively move against a traveling signal wave, arXiv, 2008.12955, 2020.08. |
| 9. | Hiroyuki Ebata, Kousuke Moriyama, Thasaneeya Kuboki, Satoru Kidoaki, General cellular durotaxis induced with cell-scale heterogeneity of matrix-elasticity, Biomaterials, 10.1016/j.biomaterials.2019.119647, 230, 2020.02, [URL], Stiffness-gradient-induced cellular taxis, so-called durotaxis, has been extensively studied on a substrate with a single broad or steep stiffness gradient. However, in actual living tissues, cells should sense cell-scaled heterogeneous elasticity distribution in the extracellular matrix. In this study, to clarify the effect of the cell-scale heterogeneity of matrix-elasticity on durotaxis, we examined the motility of different types of cells on microelastically-striped patterned gels with different cell-sized widths. We found that cells accumulated in stiff regions with specific width on cell-type-dependency, even when a stiffness gradient is too small to induce usual durotaxis with a monotonic stiffness gradient. Fibroblast cells accumulated in a wide stiff region of multicellular size, while mesenchymal stem cells localized in a narrow stiff region of single-cell size. It was revealed that durotactic activity is critically affected not only with the cell type but also with the cell-scale heterogeneity of matrix-elasticity. Based on the shape-fluctuation-based analysis of cell migration, the dynamics of the pseudopodia were found to play a key role in determining the behaviors of general durotaxis. Our results suggest that design of cell-scale heterogeneity of matrix-elasticity is pivotal in controlling directional cell migration, the spontaneous cell-patterning, and development of the tissue on the biomaterials surfaces.. |
| 10. | D. Huang and S. Kidoaki, Stiffness-optimized drug-loaded matrix for selective capture and elimination of cancer cells, J. Drug Deliv. Sci. Technol., 55, 10414, 2020.02. |
| 11. | T. Kuboki, H. Ebata, T. Matsuda, Y. Arai, T. Nagai, S. Kidoaki, Hierarchical development of motile polarity in durotactic cells just crossing an elasticity boundary, Cell Struct. Funct., 45, 33-43, 2020.02. |
| 12. | Kei Sugihara, Saori Sasaki, Akiyoshi Uemura, Satoru Kidoaki, Takashi Miura, Mechanisms of endothelial cell coverage by pericytes computational modelling of cell wrapping and in vitro experiments, Journal of the Royal Society, Interface, 10.1098/rsif.2019.0739, 17, 162, 2020.01, [URL], Pericytes (PCs) wrap around endothelial cells (ECs) and perform diverse functions in physiological and pathological processes. Although molecular interactions between ECs and PCs have been extensively studied, the morphological processes at the cellular level and their underlying mechanisms have remained elusive. In this study, using a simple cellular Potts model, we explored the mechanisms for EC wrapping by PCs. Based on the observed in vitro cell wrapping in three-dimensional PC-EC coculture, the model identified four putative contributing factors: preferential adhesion of PCs to the extracellular matrix (ECM), strong cell-cell adhesion, PC surface softness and larger PC size. While cell-cell adhesion can contribute to the prevention of cell segregation and the degree of cell wrapping, it cannot determine the orientation of cell wrapping alone. While atomic force microscopy revealed that PCs have a larger Young's modulus than ECs, the experimental analyses supported preferential ECM adhesion and size asymmetry. We also formulated the corresponding energy minimization problem and numerically solved this problem for specific cases. These results give biological insights into the role of PC-ECM adhesion in PC coverage. The modelling framework presented here should also be applicable to other cell wrapping phenomena observed in vivo.. |
| 13. | Saori Sasaki, Satoru Kidoaki, Precise design of microwrinkles through the independent regulation of elasticity on the surface and in the bulk of soft hydrogels, Polymer Journal, 10.1038/s41428-019-0299-8, 2019.12, [URL], Abstract: It is still difficult to precisely control microscopic wrinkles on the surface of functional materials, especially biomimetic soft hydrogels with an elastic modulus lower than 100 kPa. This is due to the difficulty in realizing the systematic and independent regulation of elasticity on the top surface and in the bulk of hydrogels, which is essential for the generation of surface microwrinkles. To overcome this problem, using a two-step photocrosslinking process with VIS and UV irradiation of a photocurable gelatinous sol, we developed a method for independently regulating the elastic modulus on the surface and in the bulk to obtain wrinkles on a biomimetic soft gel. Photocurable gelatin was first irradiated and crosslinked with VIS light in the presence of the water-soluble radical generator sulfonyl camphorquinone, which is effective in forming thick bulk gels. Next, the top surface of these precrosslinked gels was irradiated with UV light in the presence of surface-coated water-insoluble camphorquinone. As a result, this two-step photocrosslinking process enabled to independently control the elastic moduli of the surface and the bulk lower than 100 kPa and to generate several-micron-scale wrinkles on the soft hydrogel surface.. |
| 14. | Daoxiang Huang, Yu Nakamura, Aya Ogata, Satoru Kidoaki, Characterization of 3D matrix conditions for cancer cell migration with elasticity/porosity-independent tunable microfiber gels, Polymer Journal, 10.1038/s41428-019-0283-3, 2019.10, [URL], The mechanics and architectures of the extracellular matrix (ECM) critically influence 3D cell migration processes, such as cancer cell invasion and metastasis. Understanding the roles of mechanical and structural factors in the ECM could provide an essential basis for cancer treatment. However, it is generally difficult to independently characterize these roles due to the coupled changes in these factors in conventional ECM model systems. In this study, to solve this problem, we developed elasticity/porosity-tunable electrospun fibrous gel matrices composed of photocrosslinked gelatinous microfibers (nanometer-scale-crosslinked chemical gels) with well-regulated bonding (tens-of-micron-scale fiber-bonded gels). This system enables independent modulation of microscopic fiber elasticity and matrix porosity, i.e., the mechanical and structural conditions of the ECM. The elasticity of fibers was tuned with photocrosslinking conditions. The porosity was regulated by changing the degree of interfiber bonding. The influences of these factors of the fibrous gel matrix on the motility of MDA-MB-231 tumorigenic cells and MCF-10A nontumorigenic cells were quantitatively investigated. MDA-MB-231 cells showed the highest degree of MMP-independent invasion into the matrix composed of fibers with a Young’s modulus of 20 kPa and a low degree of interfiber bonding, while MCF-10A cells did not show invasive behavior under the same matrix conditions.. |
| 15. | Satoru Kidoaki, Frustrated differentiation of mesenchymal stem cells, Biophysical Reviews, 10.1007/s12551-019-00528-z, 11, 3, 377-382, 2019.06, [URL], Mesenchymal stem cells (MSCs) are one of the most useful cell resources for clinical application in regenerative medicine. However, standardization and quality assurance of MSCs are still essential problems because the stemness of MSCs depends on such factors as the collection method, individual differences associated with the source, and cell culture history. As such, the establishment of culture techniques which assure the stemness of MSCs is of vital importance. One important factor affecting MSCs during culture is the effect of the mechanobiological memory of cultured MSCs built up by their encounter with particular mechanical properties of the extracellular mechanical milieu. How can we guarantee that MSCs will remain in an undifferentiated state? Procedures capable of eliminating effects related to the history of the mechanical dose for cultured MSCs are required. For this problem, we have tried to establish the design of microelastically patterned cell-culture matrix which can effectively induce mechanical oscillations during the period of nomadic migration of cells among different regions of the matrix. We have previously observed before that the MSCs exposed to such a growth regimen during nomadic culture keep their undifferentiated state—with this maintenance of stemness believed due to lack of a particular regular mechanical dosage that is likely to determine a specific lineage. We have termed this situation as “frustrated differentiation”. In this minireview, I introduce the concept of frustrated differentiation of MSCs and show possibility of purposeful regulation of this phenomenon.. |
| 16. | Misato Iwashita, Hatsumi Ohta, Takahiro Fujisawa, Minyoung Cho, Makoto Ikeya, Satoru Kidoaki, Yoichi Kosodo, Brain-stiffness-mimicking tilapia collagen gel promotes the induction of dorsal cortical neurons from human pluripotent stem cells, Scientific reports, 10.1038/s41598-018-38395-5, 9, 1, 2018.12, [URL], The mechanical properties of the extracellular microenvironment, including its stiffness, play a crucial role in stem cell fate determination. Although previous studies have demonstrated that the developing brain exhibits spatiotemporal diversity in stiffness, it remains unclear how stiffness regulates stem cell fate towards specific neural lineages. Here, we established a culture substrate that reproduces the stiffness of brain tissue using tilapia collagen for in vitro reconstitution assays. By adding crosslinkers, we obtained gels that are similar in stiffness to living brain tissue (150–1500 Pa). We further examined the capability of the gels serving as a substrate for stem cell culture and the effect of stiffness on neural lineage differentiation using human iPS cells. Surprisingly, exposure to gels with a stiffness of approximately 1500 Pa during the early period of neural induction promoted the production of dorsal cortical neurons. These findings suggest that brain-stiffness-mimicking gel has the potential to determine the terminal neural subtype. Taken together, the crosslinked tilapia collagen gel is expected to be useful in various reconstitution assays that can be used to explore the role of stiffness in neurogenesis and neural functions. The enhanced production of dorsal cortical neurons may also provide considerable advantages for neural regenerative applications.. |
| 17. | Atsushi Sakai, Yoshihiro Murayama, Kei Fujiwara, Takahiro Fujisawa, Saori Sasaki, Satoru Kidoaki, Miho Yanagisawa, Increasing Elasticity through Changes in the Secondary Structure of Gelatin by Gelation in a Microsized Lipid Space, ACS Central Science, 10.1021/acscentsci.7b00625, 4, 4, 477-483, 2018.04, [URL], Even though microgels are used in a wide variety of applications, determining their mechanical properties has been elusive because of the difficulties in analysis. In this study, we investigated the surface elasticity of a spherical microgel of gelatin prepared inside a lipid droplet by using micropipet aspiration. We found that gelation inside a microdroplet covered with lipid membranes increased Young's modulus E toward a plateau value E∗ along with a decrease in gel size. In the case of 5.0 wt % gelatin gelled inside a microsized lipid space, the E∗ for small microgels with R ≤ 50 μm was 10-fold higher (35-39 kPa) than that for the bulk gel (∼3 kPa). Structural analysis using circular dichroism spectroscopy and a fluorescence indicator for ordered beta sheets demonstrated that the smaller microgels contained more beta sheets in the structure than the bulk gel. Our finding indicates that the confinement size of gelling polymers becomes a factor in the variation of elasticity of protein-based microgels via secondary structure changes.. |
| 18. | H. Ebata, A. Yamamoto, Y. Tsuji, S. Sasaki, K. Moriyama, T. Kuboki, S. Kidoaki, Persistent random deformation model of cells crawling on a gel surface, Sci. Rep., 8, 5153, 2018.03. |
| 19. | Kousuke Moriyama, Satoru Kidoaki, Cellular Durotaxis Revisited Initial-Position-Dependent Determination of the Threshold Stiffness Gradient to Induce Durotaxis, Langmuir, 10.1021/acs.langmuir.8b02529, 2018.01, [URL], Directional cell movement from a softer to a stiffer region on a culture substrate with a stiffness gradient, so-called durotaxis, has attracted considerable interest in the field of mechanobiology. Although the strength of a stiffness gradient has been known to influence durotaxis, the precise manipulation of durotactic cells has not been established due to the limited knowledge available on how the threshold stiffness gradient (TG) for durotaxis is determined. In the present study, to clarify the principles for the manipulation of durotaxis, we focused on the absolute stiffness of the soft region and evaluated its effect on the determination of TG required to induce durotaxis. Microelastically patterned gels that differed with respect to both the absolute stiffness of the soft region and the strength of the stiffness gradient were photolithographically prepared using photo-cross-linkable gelatins, and the TG for mesenchymal stem cells (MSCs) was examined systematically for each stiffness value of the soft region. As a result, the TG values for soft regions with stiffnesses of 2.5, 5, and 10 kPa were 0.14, 1.0, and 1.4 kPa/μm, respectively, i.e., TG markedly increased with an increase in the absolute stiffness of the soft region. An analysis of the area and long-axis length for focal adhesions revealed that the adhesivity of MSCs was more stable on a stiffer soft region. These results suggested that the initial location of cells starting durotaxis plays an essential role in determining the TG values and furthermore that the relationship between the position-dependent TG and intrinsic stiffness gradient (IG) of the culture substrate should be carefully reconsidered for inducing durotaxis; IG must be higher than TG (IG ≥ TG). This principle provides a fundamental guide for designing biomaterials to manipulate cellular durotaxis.. |
| 20. | K. Tamada, E. Usukura, Y. Yanase, A. Ishijima, T. Kuboki, S. Kidoaki, K. Okamoto., LSPR-mediated high axial-resolution fluorescence imaging on a silver nanoparticle sheet, PLOS One, 12, 12, e0189708, 2017.12. |
| 21. | S. Masuda, Y. Yanase, E. Usukura, S. Ryuzaki, P. Wang, K. Okamoto, T. Kuboki, S. Kidoaki, and K. Tamada, High-Resolution Imaging of a Cell-Attached Nanointerface Using a Gold-Nanoparticle Two-Dimensional Sheet, Scientific Reports, 7, 3720, 2017.06. |
| 22. | Tomo Kurimura, Yoshiko Takenaka, Satoru Kidoaki, Masatoshi Ichikawa, Fabrication of Gold Microwires by Drying Gold Nanorods Suspensions, Adv. Mater. Interf., DOI: 10.1002/admi.201601125, 1601125, 2017.04. |
| 23. | Naohiko Shimada, Minako Saito, Sayaka Shukuri, Sotaro Kuroyanagi, Thasaneeya Kuboki, Satoru Kidoaki, Takeharu Nagai, Atsushi Maruyama, Reversible monolayer/spheroid cell culture switching by UCST-type thermoresponsive ureido polymers, ACS Applied Mater. Interf., DOI: 10.1021/acsami.6b07614, 8, 31524-31529, 2016.11. |
| 24. | T. Kuboki, S. Kidoaki, Fabrication of elasticity-tunable gelatinous gel for mesenchymal stem cell culture, Methods Mol. Biol., DOI 10.1007/978-1-4939-3584-0_25, 1416, 425-441, 2016.04. |
| 25. | Fahsai Kantawong, Thasaneeya Kuboki, Satoru Kidoaki, Redox gene expression of adipose-derived stem cells in response to soft hydrogel, Turkish Journal of Biology, 39, 682-691, 2015.06. |
| 26. | Ayaka Ueki, Satoru Kidoaki, Manipulation of cell mechanotaxis by designing curvature of the elasticity boundary on hydrogel matrix, Biomaterials, 41, 45-52, 2015, 2014.12. |
| 27. | Naohiko Shimada, Satoru Kidoaki, Atsushi Maruyama, Smart hydrogels exhibiting UCST-type volume changes under physiologically relevant conditions , RSC Advances, 4, 52346, 2014, 2014.10. |
| 28. | Thasaneeya Kuboki, Wei Chen, Satoru Kidoaki, Time-dependent migratory behaviors in the long-term studies of fibroblast durotaxis on a hydrogel substrate fabricated with a soft band, Langmuir, 30, 6187-6196., 2014.06. |
| 29. | Hiroyuki Sakashita, Satoru Kidoaki, Rectified cell migration on saw-like micro-elastically patterned hydrogels with asymmetric gradient ratchet teeth, PLOS One, 8, 10, e78067, 2013.10. |
| 30. | Hiroshi Yoshikawa, Takahito Kawano, Takehisa Matsuda, Satoru Kidoaki, Motomu Tanaka, Morphology and adhesion strength of myoblast cells on photocurable gelatin under native and non-native micromechanical environments, J. Phys. Chem. Part B, 117, 4081-4088, 2013.05. |
| 31. | T. Okuda, Y. Tahara, N. Kamiya, M. Goto, and S. Kidoaki, S/O-nanodispersion electrospun fiber mesh effective for sustained release of healthy plasmid DNA with the structural and functional Integrity, Journal of Biomaterials Science: Polymer Edition, 24, 1277-1290, 2013.01. |
| 32. | M. Horning, S. Kidoaki, T. Kawano, K. Yoshikawa, Rigidity-matching between cells and the extracellular matrix leads to the stabilization of cardiac conduction, Biophys. J., 102, 379-387, 2012.02. |
| 33. | T. Okuda and S. Kidoaki, Multidrug delivery systems with single formulation ~current status and future perspective~, Journal of Biomaterials and Nanobiotechnology, 3, 50-60, 2012.01. |
| 34. | T. Kawano and S. Kidoaki, Elasticity boundary conditions required for cell mechanotaxis on microelastically-patterned gels, Biomaterials, 32: 2725-2733 (2011)., 2011.01, 細胞は弾性基材表面の硬い領域を指向して運動する性質を示す（メカノタクシス）ことが知られていたが、その駆動のための表面弾性勾配の定量的条件は確立されておらず、メカノタクシスを系統的に誘導し制御することは不可能であった。本論文ではこの問題に対して、独自の弾性率可変ヒドロゲルのマイクロ弾性パターニング技術を確立することにより、細胞のメカノタクシスの誘導条件を初めて明確にした。その技術は細胞運動を操作する培養基材設計の一般的な基礎となるものである。. |
| 35. | N. Chen, A. Zinchenko, S. Kidoaki, M. Murata, K. Yoshikawa, Thermo-Switching of the Conformation of Genomic DNA in Solutions of Poly-(N-isopropylacrylamide), Langmuir, 26, 2995-2998 (2010)., 2010.03. |
| 36. | T. Okuda, K. Tominaga, S. Kidoaki, Time-programmed dual release formulation by multilayered drug-loaded nanofiber meshes, Journal of Controlled Release, 143, 2, 258-564, 143(2), 258-564 (2010)., 2010.02. |
| 37. | F. Ito, K. Usui, D. Kawahara, A. Suenaga, T. Maki, S. Kidoaki, H. Suzuki, M. Taiji, M. Itoh, Y. Hayashizaki, T. Matsuda, Protein-peptide specific interaction-driven hydrogel formation, hydrodynamic shear stress-dependent gel-to-sol reversibility and its potential application to injectable cartilage tissue, Biomaterials, 31, 58-66 (2009)., 2009.09. |
| 38. | K. Usui, T. Maki, F. Ito, A. Suenaga, S. Kidoaki, M. Itoh, M. Taiji, T. Matsuda, Y. Hayashizaki, H. Suzuki, Nanoscale elongating control of the self-assembled protein filament with the cysteine-introduced building blocks, Protein Science, 18, 960-969, 18, 960-969 (2009)., 2009.02. |
| 39. | S. Kidoaki and T. Matsuda, Microelastic gradient gelatinous gels to induce cellular mechanotaxis, Journal of Biotechnology, 133, 225-230 (2008)., 2008.01. |
| 40. | S. Kidoaki and T. Matsuda, Shape-engineered fibroblasts: cell elasticity and actin cytoskeletal features characterized by fluorescence and atomic force microscopy, Journal of Biomedical Materials Research: Part A, 81, 728-735, 2007.06. |
| 41. | S. Kidoaki and T. Matsuda, Shape-engineered vascular endothelial cells: nitric oxide production, cell elasticity, and actin cytoskeletal features, Journal of Biomedical Materials Research: Part A, 81, 803-810, 81, 803-810 (2007)., 2007.06. |
| 42. | T. Maki, S. Kidoaki, K. Usui, H. Suzuki, M. Ito, F. Ito, Y. Hayashizaki, T. Matsuda, Dynamic force spectroscopy of the specific interaction between PDZ-domain and its recognition peptides, Langmuir, 23, 2668-2673 (2007)., 2007.01. |
| 43. | S. Kidoaki, T. Matsuda, K. Yoshikawa, Relationship between apical membrane elasticity and stress fiber organization in fibroblasts analyzed by fluorescence and atomic force microscopy, Biomechan Model Mechanobiol, 5, 263-272 (2006)., 2006.11. |
| 44. | S. Kidoaki, I.K. Kwon, T. Matsuda, Mesoscopic spatial designs of nano- and micron-fiber meshes for tissue-engineering matrix and scaffold based on newly devised multilayering and mixing electrospinning techniques, Biomaterials, 10.1016/j.biomaterials.2004.01.063, 26, 1, 37-46, 26(1), 37-46 (2005)., 2005.01. |
| 45. | S. Ohya, S. Kidoaki, T. Matsuda, Poly(N-isopropylacrylamide) (PNIPAAM)-grafted hydrogel surfaces: Interrelationship between microscopic structures and mechanical property of surface regions and cell adhesiveness, Biomaterials, 10.1016/j.biomaterials.2004.08.006, 26, 16, 3105-3111, 26, 3105-3111 (2005)., 2005.01. |
| 46. | I.K. Kwon, S. Kidoaki, T. Matsuda, Electrospun nano- to microfiber fabrics made of biodegradable copolyesters: structural characteristics, mechanical properties and cell adhesion potential, Biomaterials, 10.1016/j.biomaterials.2004.10.007, 26, 18, 3929-3939, 26(18), 3929-3939 (2005)., 2005.01. |
| 47. | T. Matsuda, M. Ihara, H. Inoguchi, I.K. Kwon, K. Takamizawa, S. Kidoaki, Mechano-active scaffold design of small-diameter artificial graft made of electrospun segmented polyurethane mesh fabrics, J. Biomed. Mater. Res. A, 10.1002/jbm.a.30260, 73A, 1, 125-131, 73, 125-131 (2005)., 2005.01. |
| 48. | S. Kidoaki, I.K. Kwon, T. Matsuda, Structural feature and mechanical property of in situ-bonded meshes of segmented polyurethane electrospun from mixed solvents, J. Biomed. Mater. Res. B, 10.1002/jbm.b.30336, 76B, 1, 219-229, 76, 219-229 (2005)., 2005.01. |
| 49. | A. Idiris, S. Kidoaki, K. Usui, T. Maki, H. Suzuki, M. Ito, M. Aoki, Y. Hayashizaki, T. Matsuda, Force measurement on antigen-antibody interaction by atomic force microscopy using photograft-polymer spacer, Biomacromolecules, 10.1021/bm0502617, 6, 5, 2776-2784, 6, 2776-2784 (2005)., 2005.01. |
| 50. | S. Kato, S. Kidoaki, T. Matsuda, Substrate-dependent Cellular Behaviors of Swiss 3T3 Fibroblasts and Activation of Rho Family during Adhesional and Spreading Processes, J. Biomed. Mater. Res, 10.1002/jbm.a.20012, 68A, 2, 314-324, 68, 314-324 (2004)., 2004.01. |
| 51. | T. Iwataki, S. Kidoaki, T. Sakaue, K. Yoshikawa, and S. S. Abramuchuk, Competition Between Compaction of Single Chains and Bundling of Multiple Chains in Giant DNA Molecules, J. Chem. Phys, 120、4004−4011 (2004)., 2004.01. |
| 52. | T. Matsuda, I.K. Kwon, S. Kidoaki, Photocurable biodegradable liquid copolymer: synthesis of acrylate-endcapped trimethylene carbonate-based prepolymers, photocuring and hydrolysis, Biomacromolecues, 10.1021/bm034231k, 5, 2, 295-305, 5、295-305 (2004)., 2004.01. |
| 53. | S. Kidoaki and K. Yoshikawa, Folding and Unfolding of a Giant Duplex-DNA in a Mixed Solution with Polycations, Polyanions, and Crowding Neutral Polymers, Biophys. Chem, 76, 133-143 (1999)., 1999.01. |
| 54. | S. Kidoaki and T. Matsuda, Adhesion Forces of the Blood Plasma Proteins on Self-Assembled Monolayer Surfaces of Alkanethiolates with Different Functional Groups Measured by an Atomic Force Microscope, Langmuir, 15, 7639-7646 (1999)., 1999.01. |
| 55. | T. Iwataki, Y. Yoshikawa, S. Kidoaki, D. Umeno, M. Kiji, M. Maeda, Cooperativity vs. Phase Transition in a Giant Single DNA Molecules, J. Am. Chem. Soc, 10.1021/ja000230d, 122, 41, 9891-9896, 122, 9891-9896 (2000)., 2000.01. |
| 56. | S. Kidoaki, Y. Nakayama, and T. Matsuda, Measuerment of Interaction Forces Between Proteins and Iniferter-Based Graft-Polymerized Surfaces with an Atomic Force Microscope in an Aqueous Media, Langmuir, 17, 1080-1087 (2001)., 2001.01. |
| 57. | S. Kidoaki, S. Ohya, Y. Nakayama, and T. Matsuda, Thermo-Responsive Property of N-isopropylacrylmide Graft-Polymerized Surfaces Measured with an Atomic Force Microscope, Langmuir, 17, 2402-2407 (2001)., 2001.01. |
| 58. | N. Yoshinaga, K. Yoshikawa, and S. Kidoaki, Multi-scaling in a long semi-flexible polymer chain in 2D, J. Chem. Phys., 10.1063/1.1475759, 116, 22, 9926-9929, 116, 9926-9929 (2002)., 2002.01. |
| 59. | S.G.Starodoubtsev, S.Kidoaki, K.Yoshikawa, Interaction of Double-stranded T4 DNA with Cationic Gel of Poly(Diallyldimethylammonium Chloride), Biomacromolecules, 10.1021/bm025583e, 4, 1, 32-37, 4, 32-37 (2003)., 2003.01. |
| 60. | T. Okuda, S. Kidoaki , M. Ohsakia, Y. Koyama, K. Yoshikawa, Time-dependent complex formation of dendritic poly(L-lysine)s with plasmid DNA and correlation with in vitro transfection efficiencies, Org. Biomol. Chem., 1, 1270-1273 (2003)., 2003.01. |
| 61. | Y. Nakayama, A. Furumoto, S. Kidoaki and T. Matsuda, Photocontrol of Cell Adhesion and Proliferation by a Photoinduced Cationic Polymer Surface, Photochem. Photobiol., 10.1562/0031-8655(2003)0772.0.CO;2, 77, 5, 480-486, 77(5), 480-486 (2003)., 2003.01. |
| 62. | T. Matsuda, J. Nagase, A. Gouda, Y. Hirano, S. Kidoaki, and Y. Nakayama, Phosphorylcholine-endcapped oligomer and block co-oligomer and surface biological reactivity, Biomaterials, 10.1016/S0142-9612(03)00344-2, 24, 24, 4517-4527, 24, 4517-4527 (2003)., 2003.01. |
| 63. | S. Kidoaki and K. Yoshikawa, The Folded State of Long Duplex-DNA Chain Reflects Its Solution History, Biophys. J., 71, 932-939 (1996)., 1996.01. |
| 64. | K. Yoshikawa, S. Kidoaki, M. Takahashi, V. V. Vasilevskaya and A. R. Khokhlov, Marked Discreteness on The Coil-Globule Transition of Single Duplex-DNA, Ber. Bunsen-Ges. Phys. Chem, 100, 876-880 (1996)., 1996.01. |
| 65. | H. Noguchi, S. Saito, S. Kidoaki and K. Yoshikawa, Self Organized Nanostructure Constructed with a Single Polymer Chain., Chem. Phys. Lett, 261, 527-533 (1996)., 1996.01. |
| 66. | V. V. Vasilevskaya, A. R. Khokhlov, S. Kidoaki and K. Yoshikawa, Structure of Collapsed Persistent Macromolecule: Toroid vs. Spherical Globule, Biopolymers, 41, 51-60 (1997)., 1997.01. |
| 67. | N. Emi, S. Kidoaki, K. Yoshikawa and H. Saito, Gene Delivery Mediated by Polyarginine Requires a Formation of Big Carrier-Complex of DNA Aggregate, Biochem. Biophys. Res. Commun, 231, 421-424 (1997)., 1997.01. |
| 68. | T. Kuboki, F. Kantawong, R. Burchmore, M.J. Dalby, and S. Kidoaki, 2D-DIGE proteomic analysis of mesenchymal stem cell cultured on the elasticity-tunable hydrogels, Cell Structure and Function, 37, 127-139,2012.. |
主要総説, 論評, 解説, 書評, 報告書等
主要学会発表等
学会活動
所属学会名
日本分子生物学会
日本メカノバイオロジー学会
日本バイオマテリアル学会
高分子学会
生物物理学会
Biophysical Society
日本化学会
細胞生物学会
日本生体医工学会
日本再生医療学会
バイオミメティックス研究会
国際メカノバイオロジー学会
学協会役員等への就任
2021.04～2022.03, 日本メカノバイオロジー学会, 理事.
2017.01～2018.12, 日本生物物理学会, 分野別専門委員（E-19. 医用生体工学）.
2018.05～2022.03, 日本生体医工学会, 代議員.
2016.06～2018.06, 日本細胞生物学会, 代議員.
2013.11～2015.11, 日本バイオマテリアル学会, 理事.
2013.04～2015.03, 日本機械学会, 分科会委員（P-SCC12 高度物理刺激と生体応答に関する研究分科会）.
2012.04, 高分子学会バイオミメティックス研究会, 運営委員.
2011.04, 日本生物物理学会, 分野別専門委員（D32 培養細胞）.
2012.04～2018.12, 日本バイオマテリアル学会, 評議員.
学会大会・会議・シンポジウム等における役割
2017.09.19～2017.09.21, 第55回日本生物物理学会年会, シンポジウムオーガナイザー.
2013.07.03～2013.07.07, 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, セッションオーガナイザー.
2010.03.11～2010.03.12, 文部科学省 科学研究費補助金 「特定領域研究」 マルチスケール操作によるシステム細胞工学（バイオ操作） 第8回公開シンポジウム, オーガナイザー.
2009.09.16～2009.09.18, 第58回高分子討論会, セッションオーガナイザー.
学術論文等の審査
| 年度 | 外国語雑誌査読論文数 | 日本語雑誌査読論文数 | 国際会議録査読論文数 | 国内会議録査読論文数 | 合計 |
|---|---|---|---|---|---|
| 2024年度 | 1 | 1 | |||
| 2023年度 | 8 | 8 | |||
| 2022年度 | 14 | 14 | |||
| 2021年度 | 13 | 13 | |||
| 2020年度 | 6 | 6 | |||
| 2019年度 | 15 | 20 | 35 | ||
| 2018年度 | 11 | 11 | |||
| 2017年度 | 17 | 17 | |||
| 2016年度 | 22 | 22 | |||
| 2015年度 | 13 | 19 | 32 | ||
| 2014年度 | 19 | 19 | |||
| 2013年度 | 23 | 23 | |||
| 2012年度 | 11 | 11 | |||
| 2011年度 | 21 | 21 | |||
| 2010年度 | 13 | 13 | |||
| 2009年度 | 11 | 11 | |||
| 2008年度 | 13 | 13 | |||
| 2007年度 | 13 | 1 | 14 | ||
| 2006年度 | 12 | 12 | |||
| 2005年度 | 5 | 5 | |||
| 2004年度 | 1 | 1 | |||
| 2003年度 | 6 | 6 | |||
| 2002年度 | 1 | 1 | |||
| 2001年度 | 1 | 1 |
その他の研究活動
外国人研究者等の受入れ状況
2013.01～2013.03, 1ヶ月以上, Chiang Mai University, Thailand, 先導物質化学研究所 招聘外国人研究員制度.
2010.03～2010.05, 1ヶ月以上, Chiang Mai University, Thailand, 日本学術振興会.
研究資金
科学研究費補助金の採択状況(文部科学省、日本学術振興会)
2021年度～2024年度, 挑戦的研究（開拓）, 代表, 細胞メカノ活性化効果を最適化する非一様力学場培養技術の開発.
2018年度～2021年度, 基盤研究(A), 代表, 流動性足場・曲面足場設計に基づくオルガノイドの精密誘導技術の開発.
2015年度～2016年度, 挑戦的萌芽研究, 代表, 弾性率可変マイクロゲルファイバーマトリックスを用いた異種細胞の自発的機能的局在化.
2015年度～2017年度, 基盤研究(B), 代表, 弾性パターニングゲルを用いたヒトiPS細胞のフィーダーフリー高速増殖技術の開発.
2012年度～2016年度, 新学術領域研究, 分担, 生物規範メカニクス・システム.
2012年度～2014年度, 基盤研究(B), 代表, 分化フラストレーション誘導基材を用いた幹細胞の未分化維持大量培養技術の開発.
2005年度～2007年度, 若手研究(A), 代表, 細胞外力学環境の微細設計と細胞のメカノタキシス制御のナノバイオメカニクス研究.
2005年度～2009年度, 特定領域研究, 分担, 組織形成のメカノバイオロジーと機能組織のロボット加工技術.
2004年度～2004年度, 萌芽研究, 代表, ナノ・マイクロファイバー骨格基材の電界紡糸設計：コンプライアント人工血管への応用.
2003年度～2004年度, 若手研究(A), 代表, 細胞形態・機能調節のための細胞一材料相互作用のナノバイオメカニクス研究.
2003年度～2003年度, 萌芽研究, 代表, エレクトロスピニングによるナノファイバー形成を応用した組織骨格基材の機能的設計.
競争的資金(受託研究を含む)の採択状況
2015年度～2020年度, 革新的先端研究開発支援事業（AMED-CREST), 代表, 幹細胞の品質保持培養のためのメカノバイオマテリアルの開発.
2010年度～2013年度, 最先端研究開発支援プロジェクト（川合最先端PJ）, 分担, 一分子解析技術を基盤とした革新的ナノバイオデバイスの開発研究.
2009年度～2012年度, 科学技術振興機構さきがけ研究, 代表, 細胞運動・機能を操作するナノ・マイクロメカニカルシステムの構築.
2003年度～2007年度, 戦略的創造研究推進事業 (文部科学省), 分担, ゲノムレベルのタンパク相互作用探索と医療に向けたナノレゴ開発（林崎CREST） 分担テーマ『リガンドーレセプタ力を活用したタンパク質分子のナノ秩序アーキテクチャー』.
1999年度～2004年度, 戦略的創造研究推進事業 (文部科学省), 分担, 自己生成するナノ秩序体：高次構造制御と機能発現(吉川CREST)
分担テーマ『高分子ナノ秩序体の光化学反応による構築と機能制御』
.
分担テーマ『高分子ナノ秩序体の光化学反応による構築と機能制御』
.
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