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Hidefumi Maeda Last modified date:2019.05.30



Graduate School


Academic Degree
DDS, Ph.D.
Country of degree conferring institution (Overseas)
No
Field of Specialization
Endodontology and Opereative Dentistry
Total Priod of education and research career in the foreign country
02years00months
Research
Research Interests
  • Development of artificial periodontal ligament using 3D printer.
    keyword : Artificial periodonatl ligament, 3D printer
    2017.04~2022.03.
  • Analysis of the mechanism of maintaining the stemness of periodontal ligament stem cells
    keyword : priodontal ligament, stemness
    2003.04.
  • Development of novel dental pulp capping materials
    keyword : direct pulp capping
    2015.04.
  • Analysis of the mechanism of senescence of dental pulp cells.
    keyword : dental pulp cells, senescence, aging
    2014.04.
  • Development of the artificial tooth root with periodontal ligament tissue.
    keyword : artificial tooth root, periodontal ligament tissue
    2017.04~2022.03.
  • Development of periodontal ligament stem cells from human iPS cells.
    keyword : periodontal ligament stem cells, iPS cells
    2017.04~2022.03.
  • Development of regenerative therapy of periodontal tissue using iPS cells.
    keyword : iPS cells
    2012.04~2022.03.
  • Development of bioactive resin with the potency of regeneration of periodontal ligament tissue
    keyword : Periodontal ligament tissue, Bioactive resin
    2013.05~2017.03.
  • Development of regenerative therapy of periodontal tissue
    keyword : periodontal stem cell
    2003.04.
  • Development of immortalized human periodontal ligament cells, cloning of them, and detection of gene specific for these cells
    keyword : periodontal cell
    2003.04~2006.03The establishment of immortal cell line of human periodontal cells, their cloning, and the detection of the gene specific for them.
  • Evaluation of the effects of human periapical granulation tissue on bone metabolism
    keyword : periapical granuloma, osteoblast
    1998.04~1999.03The elucidation about whether fibroblasts derived from humanperiapical granulation tissue have the potentials to calcify..
Current and Past Project
  • Mechanism of Transforming Growth Factor β1 to Promotes Migration of Human Periodontal Ligament Cells
  • Mineral Trioxide Aggregate (MTA) induces the osteoblastic/cementoblastic differentiation of human PDL cells to activate mineralization. We aim to disclose the mechanism of bioactive effects of MTA, and to develope the new therapy for the regeneration of human periodontal ligament.
Academic Activities
Books
1. Naohisa Wada, Atsushi Tomokiyo, Hidefumi Maeda, Future Perspectives in Dental Stem Cell Engineering and the Ethical Considerations. , Springer, Dental Stem Cells. pp 289-307, 2016.06.
2. Atsushi Tomokiyo, Naohisa Wada, Hidefumi Maeda, Contribution of Stem Cells to Dental Tissue Regeneration: Isolation, Function, and Application., Frontiiers, Frontiers in Stem Cell and Regenerative Medicine Research, Vol. 2, pp3-38, 2016.12.
3. Hidefumi Maeda, naohisa wada, Atsushi Tomokiyo, Monnouchi Satoshi, Akifumi Akamine, Prospective Potency of TGF-1 on Maintenance and Regeneration of Periodontal Tissue, Elsevier Academic Press, 283-367, 2013.06.
4. Maeda H, Tomokiyo A, Wada N, Akamine A, INDUCTION OF BMP-2 IN PERIODONTAL LIGAMENT CELLS BY CALCIUM-BASED BIOMATERIAL. In: Anja Nohe (ed) Bone Morphogenetic Proteins: New Research., Nova Science Publishers, Inc., pp187-202., 2012.02.
5. Hidefumi Maeda, Shinsuke Fujii, Satoshi Monnouchi, Naohisa Wada and Akifumi Akamine , Differentiation of Periodontal Stem/Progenitor Cells: Roles of TGF-beta1 In: M.A. Hayat (ed) Stem Cells and Cancer Stem Cells : Therapeutic Applications in Disease and Injury, Volume 4., Springer, pp51-58, 2012.05.
6. Hidefumi Maeda, Naohisa Wada, Shinsuke Fujii, Atsushi Tomokiyo & Akifumi Akamine, Periodontal ligament stem cells, InTech, In Press, 2011.08.
Reports
1. Atsushi Tomokiyo, Naohisa Wada, Hidefumi Maeda, Contribution of stem cells to dental tissue regeneration; isolation, function, and application. , Frontiers in Stem Cell and Regenerative Medicine Research Vol. 2, 2016, 3-38, 2016.07.
2. Naohisa Wada, Atsushi Tomokiyo, Hidefumi Maeda, Future Perspectives in Dental Stem Cell Engineering and the Ethical Considerations. , Dental Stem Cells. , 2016.06.
3. Hidefumi Maeda, Akifumi Akamine, Quest for the development of tooth root/periodontal ligament complex by tissue engineering. , Integr Mol Med. , 1(2): 22-25, 2014. Doi: 10.15761/IMM.1000106, 2014.10, The life-span of the tooth is intimately-associated with healthiness of periodontal ligament (PDL) which is a connective tissue situated between bone and cementum that covers tooth root surface. However, once this tissue is severely damaged by deep caries, periodontitis, and trauma, this leads to severe difficulty in its regeneration, resulting in tooth loss and decreased quality of life. The development of the therapy for generation and regeneration of the periodontal tissue is an urgent issue. Therefore, researchers have tried to improve efficiently-generative and regenerative medicine using stem cells, signal molecules, and scaffolds, requisite for tissue regeneration. In recent studies, a dental follicle tissue that is composed of stem cell population potentially differentiating into PDL tissue, cementum, and alveolar bone is of current interest. More recently a revolutionary and attractive study reporting the development of bio-hybrid implant that reserved newly-formed cementum/PDL tissue complex on its surface was introduced. In this review, we describe comprehensive reports that tried to develop the cementum/PDL complex by tissue engineering and future prospects..
4. Hidefumi Maeda, Atsushi Tomokiyo, naohisa wada, Koori Katsuaki, Giichiro Kawachi, Akifumi Akamine, Regeneration of the periodontium for preservation of the damaged tooth, Hindawi Publishing Corporation, 2014.10, The population of the world grows every year, and life expectancy tends to increase. Thus, longterm preservation of teeth in aged individuals is an urgent issue. The main causes of tooth loss are well known to be periodontitis, caries, fractures, and orthodontic conditions. Although implant placement is a widely accepted treatment for tooth loss, most patients desire to preserve their own teeth. Many clinicians and researchers are therefore challenged to treat and preserve
teeth that are irreversibly affected by deep caries, periodontitis, fractures, and trauma. Tissue engineering techniques are beneficial in addressing this issue; stem
cells, signal molecules, and scaffolds are the main elements of such techniques. In this review, we describe these three elements with respect to their validation for regeneration of the periodontium and focus particularly on the potency of diverse scaffolds. In addition, we provide a short overview of the ongoing studies of 4-methacryloxyethyl trimellitate anhydride/methyl methacrylate-tri-n-butyl-borane resin including calcium chloride or hydroxyapatite for periodontium regeneration..
5. Hidefumi Maeda, naohisa wada, Atsushi Tomokiyo, Monnouchi Satoshi, Akifumi Akamine, Prospective Potency of TGF-1 on Maintenance and Regeneration of Periodontal Tissue., Elsevier Adadmic Press, 2013.06.
6. Hidefumi Maeda, Shinsuke Fujii, Atsushi Tomokiyo, Naohisa Wada and Akifumi Akamine, Potentials of periodontal ligament stem/progenitor cell lines in regeneration studies, Quintessence, 2011.12.
7. Hidefumi Maeda, Atsushi Tomokiyo, Shinsuke Fujii, Naohisa Wada and Akifumi Akamine, Promise of periodontal ligament stem cells, BioMed Central, 2011.07.
Papers
1. Arima M, Hasegawa D, Yoshida S, Mitarai H, Tomokiyo A, Hamano S, Sugii H, Wada N, Maeda H., R-spondin 2 promotes osteoblastic differentiation of immature human periodontal ligament cells through the Wnt/-catenin signaling pathway., J Periodont Res., 10.1111/jre.12611, 54, 2, 143-153, 2019.02.
2. Nozu A, Hamano S, Tomokiyo A, Hasegawa D, Sugii H, Yoshida S, Mitarai H, Taniguchi S, Wada N, Maeda H., Senescence and odontoblastic differentiation of dental pulp cells., J Cell Physiol, 10.1002/jcp.26905, 234, 1, 849-859, ??, 2019.01.
3. Tomokiyo A, Yoshida S, Hamano S, Hasegawa D, Sugii H, Maeda H., Detection, Characterization, and Clinical Application of Mesenchymal Stem Cells in Periodontal Ligament Tissue, Stem Cells International, ??, 2018.08.
4. Hamano S, Tomokiyo A, Hasegawa D, Yoshida S, Sugii H, Mitarai H, Fujino S, Wada N, Maeda H., Extracellular Matrix from Periodontal Ligament Cells Could Induce the Differentiation of Induced Pluripotent Stem Cells to Periodontal Ligament Stem Cell-Like Cells., Stem Cells Dev., 10.1089/scd.2017.0077, 15;27, 2, 100-111, 2018.01, The periodontal ligament (PDL) plays an important role in anchoring teeth in the bone socket. Damage to the PDL, such as after severe inflammation, can be treated with a therapeutic strategy that uses stem cells derived from PDL tissue (PDLSCs), a strategy that has received intense scrutiny over the past decade. However, there is an insufficient number of PDLSCs within the PDL for treating such damage. Therefore, we sought to induce the differentiation of induced pluripotent stem (iPS) cells into PDLSCs as an initial step toward PDL therapy. To this end, we first induced iPS cells into neural crest (NC)-like cells. We then captured the p75 neurotrophic receptor-positive cells (iPS-NC cells) and cultured them on an extracellular matrix (ECM) produced by human PDL cells (iPS-NC-PDL cells). These iPS-NC-PDL cells showed reduced expression of embryonic stem cell and NC cell markers as compared with iPS and iPS-NC cells, and enrichment of mesenchymal stem cell markers. The cells also had a higher proliferative capacity, multipotency, and elevated expression of PDLrelated markers than iPS-NC cells cultured on fibronectin and laminin (iPS-NC-FL cells) or ECM produced by human skin fibroblast cells (iPS-NC-SF cells). Overall, we present a culture method to produce high number of PDLSC-like cells from iPS cells as a first step toward a strategy for PDL regeneration..
5. Hasegawa D, Wada N, Yoshida S, Mitarai H, Arima M, Tomokiyo A, Hamano S, Sugii H, Maeda H., Wnt5a Suppresses Osteoblastic Differentiation of Human Periodontal Ligament Stem Cell-like Cells Via Ror2/JNK signaling., J Cell Physiol., 10.1002/jcp.26086., 233, 2, 1752-1762-1762, 2018.02.
6. Hiroyuki Mizumachi, Shinichiro Yoshida, Atsushi Tomokiyo, Daigaku Hasegawa, Sayuri Hamano, Asuka Yuda, 杉井 英樹, Suguru Serita, Hiromi Mitarai, Koori Katsuaki, Naohisa Wada, Hidefumi Maeda, Calcium-sensing receptor-ERK signaling promotes odontoblastic differentiation of human dental pulp cells., 10.1016/j.bone.2017.05.012, 101, 191-201, 2017.05.
7. Hiromi Mitarai, Naohisa Wada, Daigaku Hasegawa, Shinichiro Yoshida, Mai Arima, Atsushi Tomokiyo, Sayuri Hamano, Suguru Serita, Hiroyuki Mizumachi, Hidefumi Maeda, Transgelin mediates TGF-β1-induced proliferation of human periodontal ligament cells., J Peirodont Res, 2017.06.
8. Suguru Serita, Atsushi Tomokiyo, Daigaku Hasegawa, Sayuri Hamano, 杉井 英樹, Shinichiro Yoshida, Hiroyuki Mizumachi, Hiromi Mitarai, Monnouchi Satoshi, Naohisa Wada, Hidefumi Maeda, Transforming growth factor-β-induced gene product-h3 inhibits odontoblastic differentiation of dental pulp cells., Arch Oral Biol., 10.1016/j.archoralbio.2017.02.018, 78, 135-143, 2017.05.
9. Atsushi Tomokiyo, Naohisa Wada, Sayuri Hamano, Daigaku Hasegawa, Hideki Sugii, Shinichiro Yoshida, Hidefumi Maeda, Periodontal Ligament Stem Cells in Regenerative Dentistry for Periodontal Tissues. , 10.15406/jsrt.2015.01.00019, 1, 3, 00019, 2016.07.
10. Shinichiro Yoshida, Naohide Yamamoto, Naohisa Wada, Atsushi Tomokiyo, Daigaku Hasegawa, Sayuri Hamano, Hiromi Mitarai, Monnouchi Satoshi, Asuka Yuda, Hidefumi Maeda, Glial cell line-derived neurotrophic factor from human periodontal ligament cells treated with proinflammatory cytokines promotes neurocytic differentiation of PC12 cells., J Cell Biochem., 10.1002/jcb.25662, 118, 4, 699-708, 2017.04.
11. Shinichiro Yoshida, Naohisa Wada, Daigaku Hasegawa, Miyaji, Hiromi Mitarai, Atsushi Tomokiyo, Sayuri Hamano, Hidefumi Maeda, Semaphorin 3A Induces Odontoblastic Phenotype in Dental Pulp Stem Cells. , J Dent Res., 10.1177/0022034516653085, 95, 11, 1282-1290, 2016.09, In cases of pulp exposure due to deep dental caries or severe traumatic injuries, existing pulp-capping materials have a limited ability
to reconstruct dentin-pulp complexes and can result in pulpectomy because of their low potentials to accelerate dental pulp cell
activities, such as migration, proliferation, and differentiation. Therefore, the development of more effective therapeutic agents has been
anticipated for direct pulp capping. Dental pulp tissues are enriched with dental pulp stem cells (DPSCs). Here, the authors investigated
the effects of semaphorin 3A (Sema3A) on various functions of human DPSCs in vitro and reparative dentin formation in vivo in a rat
dental pulp exposure model. Immunofluorescence staining revealed expression of Sema3A and its receptor Nrp1 (neuropilin 1) in rat
dental pulp tissue and human DPSC clones. Sema3A induced cell migration, chemotaxis, proliferation, and odontoblastic differentiation
of DPSC clones. In addition, Sema3A treatment of DPSC clones increased β-catenin nuclear accumulation, upregulated expression of
the FARP2 gene (FERM, RhoGEF, and pleckstrin domain protein 2), and activated Rac1 in DPSC clones. Furthermore, in the rat dental
pulp exposure model, Sema3A promoted reparative dentin formation with dentin tubules and a well-aligned odontoblast-like cell layer at
the dental pulp exposure site and with novel reparative dentin almost completely covering pulp tissue at 4 wk after direct pulp capping.
These findings suggest that Sema3A could play an important role in dentin regeneration via canonical Wnt/β-catenin signaling. Sema3A
might be an alternative agent for direct pulp capping, which requires further study..
12. Monnouchi Satoshi, Hidefumi Maeda, Asuka Yuda, Suguru Serita, Naohisa Wada, Atsushi Tomokiyo, Akifumi Akamine, Benzo[a]pyrene/aryl hydrocarbon receptor signaling inhibits osteoblastic differentiation and collagen synthesis of human periodontal ligament cells., J Periodont. Res., 10.1111/jre.12355, 51, 6, 779-788, 2016.06, Background and Objective: Cigarette smoking have detrimental effects on periodontal tissue, and is known to be a risk factor for periodontal disease, including the loss of alveolar bone and ligament tissue. However, the direct effects of cigarette smoking on periodontal tissue remain unclear. Recently, we demonstrated that benzo(a)pyrene (BaP), which is a prototypic member of polycyclic aryl hydrocarbons and forms part of the content of cigarettes, attenuated the expression of extracellular matrix remodeling-related genes in human periodontal ligament (PDL) cells (HPDLCs). Thus, we aimed to examine the effects of BaP on the osteoblastic differentiation and collagen synthesis of HPDLCs.
Materials and Methods: HPDLCs were obtained from healthy molars of three patients, and quantitative RT-PCR were performed for gene expression analyses of cytochrome P450 1A1 and 1B1, alkaline phosphatase (ALP), bone sialoprotein, and aryl hydrocarbon receptor (AhR), a receptor for polycyclic aryl hydrocarbons. We have also analyzed the role of the AhR, using 2-methyl-2H-pyrazole-3-carboxylic acid (2-methyl-4-o-tolylazo-phenyl)-amide (CH-223191), which is an AhR antagonist.
Results: The treatment of HPDLCs with BaP reduced mRNA expression of osteogenic genes, ALP activity, mineralization, and collagen synthesis. The treatment with CH-223191 subsequently restored the observed suppressive effects of BaP on HPDLCs.
Conclusions: The present results suggest that BaP exerts inhibitory effects on the maintenance of homeostasis in human PDL tissue, such as osteoblastic differentiation and collagen synthesis of HPDLCs, and that this signaling pathway could be suppressed by preventing the transactivity of AhR. Future studies may unveil a role for the inhibition of AhR as a promising therapeutic agent for periodontal disease caused by cigarette smoking..
13. Daigaku Hasegawa, Naohisa Wada, Hidefumi Maeda, Shinichiro Yoshida, Hiromi Mitarai, Atsushi Tomokiyo, Monnouchi Satoshi, Sayuri Hamano, Asuka Yuda, Akifumi Akamine, Wnt5a Induces Collagen Production by Human Periodontal Ligament Cells through Transforming Growth Factor β1-mediated Upregulation of Periostin Expression., J Cell Physiol., 10.1002/jcp.24950., 230, 11, 2647-2660, 2015.11, Wnt5a, a member of the noncanonicalWnt proteins, is known to play important roles in the development of various organs and in postnatal cell functions. However, little is known about the effects of Wnt5a on human periodontal ligament (PDL) cells. In this study, we examined the localization and potential function of Wnt5a in PDL tissue. Immunohistochemical analysis revealed that Wnt5a was expressed predominantly in rat PDL tissue. Semi-quantitative reverse-transcription polymerase chain reaction and western blotting analysis demonstrated that human PDL cells (HPDLCs) expressed Wnt5a and its receptors (Ror2, Fzd2, Fzd4, and Fzd5). Removal of occlusal pressure by extraction of opposing teeth decreased Wnt5a expression in rat PDL tissue, and the expression of Wnt5a and its receptors in HPDLCs was upregulated by exposure to mechanical stress. Stimulation with Wnt5a significantly enhanced the proliferation and migration of HPDLCs. Furthermore, Wnt5a suppressed osteoblastic differentiation of HPDLCs cultivated in osteogenic induction medium, while it significantly enhanced the expression of PDL-related genes, such as periostin, type-I collagen, and fibrillin-1 genes, and the production of collagen in HPDLCs cultivated in normal medium. Both knockdown of periostin gene expression by siRNA and inhibition of TGFβ1 function by neutralizing antibody suppressed the Wnt5a-induced PDL-related gene expression and collagen production in HPDLCs. Interestingly, in HPDLCs cultured with Wnt5a, TGFβ1 neutralizing antibody significantly suppressed periostin expression, while periostin siRNA had no effect on TGFβ1 expression. These results suggest that Wnt5a expressed in PDL tissue plays specific roles in inducing collagen production by PDL cells through TGFβ1-mediated upregulation of periostin expression. This article is protected by copyright. All rights reserved..
14. Myna N. Zakaria, Toru Takeshita, Yukie Shibata, Hidefumi Maeda, Naohisa Wada, Akifumi Akamine, Yoshihisa Yamashita, Microbial community in persistent apical periodontitis: a 16S rRNA gene clone library analysis., Int Endod J., 10.1111/iej.12361, 48, 8, 717-728, 2015.08, AIM:

To characterize the microbial composition of persistent periapical lesions of root filled teeth using a molecular genetics approach.
METHODOLOGY:

Apical lesion samples were collected from 12 patients (23-80 years old) who visited the Kyushu University Hospital for apicectomy with persistent periapical lesions associated with root filled teeth. DNA was directly extracted from each sample and the microbial composition was comprehensively analysed using clone library analysis of the 16S rRNA gene. Enterococcus faecalis, Candida albicans and specific fimA genotypes of Porphyromonas gingivalis were confirmed using polymerase chain reaction (PCR) analysis with specific primers.
RESULTS:

Bacteria were detected in all samples, and the dominant findings were P. gingivalis (19.9%), Fusobacterium nucleatum (11.2%) and Propionibacterium acnes (9%). Bacterial diversity was greater in symptomatic lesions than in asymptomatic ones. In addition, the following bacteria or bacterial combinations were characteristic to symptomatic lesions: Prevotella spp., Treponema spp., Peptostreptococcaceae sp. HOT-113, Olsenella uli, Slackia exigua, Selemonas infelix, P. gingivalis with type IV fimA, and a combination of P. gingivalis, F. nucleatum, and Peptostreptococcaceae sp. HOT-113 and predominance of Streptococcus spp. On the other hand, neither Enterococcus faecalis nor C. albicans were detected in any of the samples.
CONCLUSION:

Whilst a diverse bacterial species were observed in the persistent apical lesions, some characteristic patterns of bacterial community were found in the symptomatic lesions. The diverse variation of community indicates that bacterial combinations as a community may cause persistent inflammation in periapical tissues rather than specific bacterial species..
15. Hidefumi Maeda, Akifumi Akamine, Quest for the development of tooth root/periodontal ligament complex by tissue engineering., Integr Mol Med., 10.15761/IMM.1000106, 1, 2, 22-25, 2014.10, The life-span of the tooth is intimately-associated with healthiness of periodontal ligament (PDL) which is a connective tissue situated between bone and cementum
that covers tooth root surface. However, once this tissue is severely damaged by deep caries, periodontitis, and trauma, this leads to severe difficulty in its regeneration,
resulting in tooth loss and decreased quality of life. The development of the therapy for generation and regeneration of the periodontal tissue is an urgent issue.
Therefore, researchers have tried to improve efficiently-generative and regenerative medicine using stem cells, signal molecules, and scaffolds, requisite for tissue
regeneration. In recent studies, a dental follicle tissue that is composed of stem cell population potentially differentiating into PDL tissue, cementum, and alveolar
bone is of current interest. More recently a revolutionary and attractive study reporting the development of bio-hybrid implant that reserved newly-formed cementum/
PDL tissue complex on its surface was introduced. In this review, we describe comprehensive reports that tried to develop the cementum/PDL complex by tissue
engineering and future prospects..
16. Asuka Yuda, Hidefumi Maeda, Fujii Shinsuke, Monnouchi Satoshi, Naohide Yamamoto, naohisa wada, Koori Katsuaki, Atsushi Tomokiyo, Sayuri Hamano, Daigaku Hasegawa, Akifumi Akamine, Effect of CTGF/CCN2 on osteo/cementoblastic and fibroblastic differentiation of a human periodontal ligament stem/progenitor cell line., J Cell Physiol, 10.1002/jcp.24693., 230, 1, 150-159, in press, 2015.01.
17. Hideki Sugii, Hidefumi Maeda, Atsushi Tomokiyo, Naohide Yamamoto, naohisa wada, Koori Katsuaki, Daigaku Hasegawa, Sayuri Hamano, Asuka Yuda, Monnouchi Satoshi, Akifumi Akamine, Effects of Activin A on the phenotypic properties of human periodontal ligament cells., Bone, 10.1016/j.bone.2014.05.021, 66, 62-71, 2014.07.
18. Monnouchi Satoshi, Hidefumi Maeda, Asuka Yuda, Sayuri Hamano, naohisa wada, Atsushi Tomokiyo, Koori Katsuaki, Hideki Sugii, Suguru Serita, Akifumi Akamine, Mechanical induction of interleukin-11 regulates osteo/cementoblastic differentiation of human periodontal ligament stem/progenitor cells., J Periodont Res, 10.1111/jre.12200., 50, 2, 231-239, 2015.02.
19. Hidefumi Maeda, Atsushi Tomokiyo, naohisa wada, Koori Katsuaki, GIICHIRO KAWACHI, Akifumi Akamine, Regeneration of the periodontium for preservation of the damaged tooth, Histol Histopathol, 29, 10, 1249-1262, 2014.10.
20. Yoko Teramatsu, Hidefumi Maeda, Hideki Sugii, Atsushi Tomokiyo, Sayuri Hamano, naohisa wada, Asuka Yuda, Naohide Yamamoto, Koori Katsuaki, Akifumi Akamine, Expression and effects of epidermal growth factor on human periodontal ligament cells, Cell Tissue Res., 10.1007/s00441-014-1877-x., 357, 3, 633-643, 2014.03.
21. Koori Katsuaki, Hidefumi Maeda, Fujii Shinsuke, Atsushi Tomokiyo, GIICHIRO KAWACHI, Daigaku Hasegawa, Sayuri Hamano, Hideki Sugii, naohisa wada, Akifumi Akamine, The roles of calcium-sensing receptor and calcium channel in osteogenic differentiation of undifferentiated periodontal ligament cells. , Cell Tissue Res, 10.1007/s00441-014-1918-5, 357, 3, 707-718, 2014.03.
22. naohisa wada, Hidefumi Maeda, Daigaku Hasegawa, Gronthos S, Bartold PM, Menicanin D, Fujii Shinsuke, Shinichiro Yoshida, Atsushi Tomokiyo, Monnouchi Satoshi, Akifumi Akamine, Semaphorin 3A induces mesenchymal stem-like properties in human periodontal ligament cells, Stem Cell Dev., 2014.05.
23. Takashi Tstsumi, Hiroshi Kajiya, Teruhisa Fukawa, Mina Sasaki, Tetsuomi Nemoto, Takashi Tsuzuki, Yutaka Takahashi, Fujii Shinsuke, Hidefumi Maeda, Koji Okabe, The Potential Role of TRPA1 as a Mechanoreceptor in Human Periodontal Ligament Cells. , Eur J Oral Sci, 121, 6, 538-544, 2013.06.
24. 河野 清美, 前田 英史, 藤井 慎介, 友清 淳, 山本直秀, 和田 尚久, 門野内 聡, 寺松陽子, 濱野さゆり, 郡勝明, 赤峰 昭文, Exposure to transforming growth factor-β1 after basic fibroblast growth factor promotes the fibroblastic differentiation of human periodontal ligament stem/progenitor cell lines., Cell Tissue Res., 352, 2, 249-263, 2013.02.
25. Tomokiyo A, Maeda H, Fujii S, Monnouchi S, Wada N, Hori K, Koori K, Yamamoto N, Teramatsu Y, Akamine A., Alternation of extracellular matrix remodeling and apoptosis by activation of the aryl hydrocarbon receptor pathway in human periodontal ligament cells. , J Cell Biochem., 113, 10, 3093-3103, 2012.10.
26. Yamamoto N, Maeda H, Tomokiyo A, Fujii S, Wada N, Monnouchi S, Kono K, Koori K, Teramatsu Y, Akamine A., Expression and effects of glial cell line-derived neurotrophic factor on periodontal ligament cells. , J Clin Periodontol., 39, 6, 556-564, 2012.06, AIM:

To investigate Glial cell line-derived neurotrophic factor (GDNF) expression in normal and wounded rat periodontal ligament (PDL) and the effects of GDNF on human PDL cells (HPDLCs) migration and extracellular matrix expression in HPDLCs.

MATERIAL AND METHODS:

The expression of GDNF and GDNF receptors was examined by immunocyto/histochemical analyses. Gene expression in HPDLCs treated with GDNF, interleukin-1 beta (IL-1β), or tumour necrosis factor-alpha (TNF-α) was quantified by quantitative RT-PCR (qRT-PCR). In addition, we examined the migratory effect of GDNF on HPDLCs.

RESULTS:

GDNF was expressed in normal rat PDL and cultured HPDLCs. HPDLCs also expressed GDNF receptors. In wounded rat PDL, GDNF expression was up-regulated. QRT-PCR analysis revealed that IL-1β and TNF-α significantly increased the expression of GDNF in HPDLCs. Furthermore, GDNF induced migration of HPDLCs, which was blocked by pre-treatment with the peptide including Arg-Gly-Asp (RGD) sequence, or neutralizing antibodies against integrin αVβ3 or GDNF. Also, GDNF up-regulated expression of bone sialoprotein (BSP) and fibronectin in HPDLCs.

CONCLUSIONS:

GDNF expression is increased in rat wounded PDL tissue and HPDLCs treated with pro-inflammatory cytokines. GDNF enhances the expression of BSP and fibronectin, and migration in an RGD-dependent manner via the integrin αVβ3. These findings suggest that GDNF may contribute to wound healing in PDL tissue..
27. 前田 英史, 藤井 慎介, 友清 淳, 和田 尚久, 赤峰 昭文, Periodontal tissue engineering: defining the triad., Int J Oral Maxillofac Implants., 28, 6, e461-e471, 2013.11, The idea that somatic stem cells are localized in periodontal ligament (PDL) tissues as PDL stem
cells (PDLSCs) responsible for construction and reconstruction of the periodontium has been
widely accepted. Many dental scientists have attempted to clarify the identity of these PDLSCs,
but the number of PDLSCs localized in PDL tissues is too small to be routinely and conveniently
analyzed. Therefore, researchers have been attempting to develop undifferentiated PDL cell
lines by transducing them with genes that are suitable for immortalization. The present authors
were the first to succeed in establishing two clonal human PDL stem/progenitor cell lines that
possessed multipotency derived from PDL tissues and that expressed PDL-related molecules as
well as neural crest– and embryonic stem–related markers. The differentiation stages of these
cell lines appeared to vary based on their potential to differentiate into other lineage cells, their
response to tissue regeneration–related cytokines, and their behavior when transplanted into
immunodeficient rats. This review describes the phenotypes of these cell lines compared with
reported PDLSCs or other MSCs and discusses contemporary circumstances related to PDL
regenerative medicine. Differential analyses between these two clones will reveal the mechanism
of differentiation of PDLSCs as well as their phenotypes. The results will also allow for the
acquisition of a mass population of PDLSCs or other stem cells directed toward PDL-lineage
cells and to develop an unmet treatment needed for construction and reconstruction of PDL
tissues based on tissue engineering techniques..
28. Tomokiyo A, Maeda H, Fujii S, Monnouchi S, Wada N, Kono K, Koori K, Yamamoto N, Teramatsu Y, Akamine A., A multipotent clonal human periodontal ligament cell line with neural crest cell phenotypes promotes neurocytic differentiation, migration, and survival. , J Cell Physiol., 227, 5, 2040-2050, 2012.02, Repair of injured peripheral nerve is thought to play important roles in tissue homeostasis and regeneration. Recent
experiments have demonstrated enhanced functional recovery of damaged neurons by some types of somatic stem
cells. It remains unclear, however, if periodontal ligament (PDL) stem cells possess such functions. We recently
developed a multipotent clonal human PDL cell line, termed cell line 1-17. Here, we investigated the effects of this
cell line on neurocytic differentiation, migration, and survival. This cell line expressed the neural crest cell marker
genes Slug, SOX10, Nestin, p75NTR, and CD49d and mesenchymal stem cell–related markers CD13, CD29, CD44,
CD71, CD90, CD105, and CD166. Rat adrenal pheochromocytoma cells (PC12 cells) underwent neurocytic
differentiation when co-cultured with cell line 1-17 or in conditioned medium from cell line 1-17 (1-17CM).
ELISA analysis revealed that 1-17CM contained approximately 50 pg/mL nerve growth factor (NGF). Cell line
1-17 induced migration of PC12 cells, which was inhibited by a neutralizing antibody against NGF. Furthermore,
1-17CM exerted antiapoptotic effects on differentiated PC12 cells as evidenced by inhibition of neurite retraction,
reduction in annexin V and caspase-3/7 staining, and induction of Bcl-2 and Bcl-xL mRNA expression. Thus, cell
line 1-17 promoted neurocytic differentiation, migration, and survival through secretion of NGF and possibly
synergistic factors. PDL stem cells may play a role in peripheral nerve reinnervation during PDL regeneration..
29. Maeda H, Tomokiyo A, Fujii S, Wada N, Akamine A., Promise of periodontal ligament stem cells in regeneration of periodontium. , Stem Cell Res Ther. 2(4):33, 2011., 2, 4, 1-3, 2011.07, A great number of patients around the world
experience tooth loss that is attributed to irretrievable
damage of the periodontium caused by deep caries,
severe periodontal diseases or irreversible trauma.
The periodontium is a complex tissue composed
mainly of two soft tissues and two hard tissues; the
former includes the periodontal ligament (PDL) tissue
and gingival tissue, and the latter includes alveolar
bone and cementum covering the tooth root. Tissue
engineering techniques are therefore required for
regeneration of these tissues. In particular, PDL is
a dynamic connective tissue that is subjected to
continual adaptation to maintain tissue size and width,
as well as structural integrity, including ligament
fi bers and bone modeling. PDL tissue is central in
the periodontium to retain the tooth in the bone
socket, and is currently recognized to include somatic
mesenchymal stem cells that could reconstruct the
periodontium. However, successful treatment using
these stem cells to regenerate the periodontium
effi ciently has not yet been developed. In the present
article, we discuss the contemporary standpoints
and approaches for these stem cells in the fi eld of
regenerative medicine in dentistry..
30. Biocompatibility of resin-based sealers- Comparison of Superbond sealer with AH Plus -.
31. Maeda H, Tomokiyo A, Koori K, Monnouchi S, Fujii S, Wada N, Kono K, Yamamoto N, Saito T, Akamine A. , An in vitro evaluation of two resin-based sealers on proliferation and differentiation of human periodontal ligament cells., Int Endod J, 44, 5, 425-431, 2011.05.
32. Kwon SM, Kim SA, Fujii S, Maeda H, Ahn SG, Yoon JH. , Transforming Growth Factor β1 Promotes Migration of Human Periodontal Ligament Cells through Heat Shock Protein 27 Phosphorylation., Biol Pharm Bull, 34, 4, 486-489, 2011.04.
33. Monnouchi S, Maeda H, Fujii S, Tomokiyo A, Hori K, Akamine A, The roles of angiotensin II in stretched periodontal ligament cells., J Dent Res, 90, 2, 181-185, 2011.02.
34. Fujii S, Maeda H, Tomokiyo A, Satoshi M, Hori K, Wada N, Akamine A., The effects of TGF-β1 on the proliferation and differentiation of human periodontal ligament cells and a human PDL stem/progenitor cell line., Cell Tissue Res, 342, 2, 233-242, 2010.12.
35. Maeda H, Nakano T, Tomokiyo A, Fujii S, Wada N, Monnouchi S, Hori K, Akamine A., Mineral Trioxide Aggregate Induces Bone Morphogenetic Protein-2 Expression and Calcification in Human Periodontal Ligament Cells., J Endod, 36巻, 4号, 647-652, 2010.04.
36. Yasuda Y, Tatematsu Y, Fujii S, Maeda H, Akamine A, Torabinejad M, Saito T., Effect of MTAD on the differentiation of osteoblast-like cells., J Endod, 36巻, 2号, 260-263, 2010.02.
37. The Effects of MTA on Human Periodontal Ligament Cells..
38. Yasuda Y, Inuyama H, Maeda H, Akamine A, Nör JE, Saito T., Cytotoxicity of one-step dentin-bonding agents toward dental pulp and odontoblast-like cells., J Oral Rehabil., 35(12):940-6, 2008, 2008.12.
39. Kano Y, Horie N, Doi S, Aramaki F, Maeda H, Hiragami F, Kawamura K, Motoda H, Koike Y, Akiyama J, Eguchi S, Hashimoto K., Artepillin C derived from propolis induces neurite outgrowth in PC12m3 cells via ERK and p38 MAPK pathways, Neurochem Res, 33(9):1795-803 2008, 2008.09.
40. Tomokiyo A, Maeda H, Fujii S, Wada N, Shima K, Akamine A., Development of a multipotent clonal human periodontal ligament cell line., Differentiation, 76(4):337-347, 2008.04.
41. Fujii S, Maeda H, Wada N, Tomokiyo A, Saito M, Akamine A, Investigating a clonal human periodontal ligament progenitor/stem cell line in vitro and in vivo., J Cell Physiol , 215(3):743-749, 2008.03.
42. SEM images of root canal dentin irrigated with EDTA and NaOCl - Comparison with ultrasonic irrigation -.
43. Effects of root-end filling materials on the osteoblast-like differentiation of human periodontal ligament cells.
44. Lu G, Maeda H, Reddy SV, Kurihara N, Leach R, Anderson JL, Roodman GD., Cloning and characterization of the annexin II receptor on human marrow stromal cell., J Biol Chem, 281(41): 30542-30550, 2006.01.
45. Fujii S, Maeda H, Wada N, Kano Y, Akamine A., Establishing and characterizing human periodontal ligament fibroblasts immortalized by SV40T-antigen and hTERT gene transfer., Cell Tissue Res., 324(1): 117-125, 2006.01.
46. Maeda H, Wada N, Fujii S, Akamine A., Fibroblastic cells from human periapical granulation tissue preferentially form calcified matrices in decalcified and boiled rat bone., Cell Tissue Res., 10.1007/s00441-004-1052-x, 320, 1, 135-140, 324(1): 117-125, 2005.04.
47. Wada N, Maeda H, Yoshimine Y, Akamine A., Lipopolysaccharide stimulates osteoprotegerin and receptor activator of NF-kappa B ligand in periodontal ligament fibroblasts through the induction IL-1 beta and TNF-alpha., Bone, 10.1016/j.bone.2004.04.023, 35, 3, 629-635, 35 (3) 629-635., 2004.09.
48. Maeda H, Wada N, Nakamuta H, Akamine A., Human periapical granulation tissue contains osteogenic cells., Cell and Tissue Research, 10.1007/s00441-003-0832-z, 315, 2, 203-208, 315 (2): 203-208, 2004.02.
49. Koide M, Maeda H, Roccisana JL, Kawanabe N, Reddy SV., Cytokine regulation and the signaling mechanism of osteoclast inhibitory peptide-1 (OIP-1/hSca) to inhibit osteoclast formation., Journal of Bone and Mineral Research, 18 (3): 458-465, 2003.01.
50. Goto T, Maeda H, Tanaka T., A selective inhibitor of matrix metalloproteases inhibits the migration of isolated osteoclasts by increasing the life span of podosomes., Journal of Bone and Mineral Metabolism, 10.1007/s007740200013, 20, 2, 98-105, 20 (2): 98-105, 2002.01.
51. Koide M, Kurihara N, Maeda H, Reddy SV., Identification of the Functional Domain of Osteoclast Inhibitory Peptide-1/hSca., Journal of Bone and Mineral Research, 17 (1): 111-118, 2002.01.
52. Kurihara N, Menaa C, Maeda H, Haile DJ, Reddy SV., Osteoclast-stimulating Factor Interacts with the Spinal Muscular Atrophy Gene Product to Stimulate Osteoclast Formation., Journal of Biological Chemistry, 276 (44): 41035-41039, 2001.01.
53. Wada N, Maeda H, Tanabe K, Tsuda E, Yano K, Nakamuta H, and Akamine A., Periodontal ligament cells secrete the factor that inhibits osteoclastic differentiation and function: the factor is osteoprotegerin / osteoclastogenesis inhibitory factor., Journal of Periodontal Research, 10.1034/j.1600-0765.2001.00604.x, 36, 1, 56-63, 36 (1): 56-63, 2001.01.
54. Menaa C, Reddy SV, Kurihara N, Maeda H, Anderson D, Cundy T, Cornish J, Singer FR, Bruder JM, Roodman GD., Enhanced RANK ligand expression and responsivity of bone marrow cells in Paget's disease of bone., Journal of Clinical Investigation, 105 (12): 1833-1838, 2000.01.
55. Kukita A, Kukita T, Ouchida M, Maeda H, Yatsuki H, and Kohashi O., Osteoclast-derived zinc finger (OCZF) protein with POZ domain, a possible transcriptional repressor, is involved in osteoclastogenesis., Blood, 94, 6, 1987-1997, 94 (6): 1987-1997, 1999.01.
56. Tanabe K, Nakanishi H, Maeda H, Nishioku T, Hashimoto K, Liou SY, Akamine A, and Yamamoto K., A Predominant Apoptotic Death Pathway of Neuronal PC12 Cells Induced by Activated Microglia Is Displaced by A Non-apoptotic Death Pathway Following Blockage of Caspase-3-dependent Cascade., Journal of Biological Chemistry, 10.1074/jbc.274.22.15725, 274, 22, 15725-15731, 274 (22): 15725-15731, 1999.01.
57. Maeda H, Akasaki K, Yoshimine Y, Akamine A, and Yamamoto K., Limited and Selective Localization of the Lysosomal Membrane Glycoproteins LGP85 and LGP96 in Rat Osteoclasts., Histochemistry and Cell Biology, 10.1007/s004180050354, 111, 4, 245-251, 111 (4): 245-251, 1999.01.
58. Maeda H, Hashiguchi I, Nakamuta H, Toriya Y, Wada N, and Akamine A., Histological Study of Periapical Tissue Healing in the Rat Molar after Retrofilling with Various Materials., Journal of Endodontics, 10.1016/S0099-2399(99)80397-5, 25, 1, 38-42, 25 (1): 38-42, 1999.01.
59. Kukita T, Kukita A, Xu L, Maeda H, and Iijima T, Successful Detection of Active Osteoclasts In Situ by Systemic Administration of an Osteoclast-Specific Monoclonal Antibody., Calcified Tissue International, 10.1007/s002239900506, 63, 2, 148-153, 63 (2): 148-153, 1998.01.
60. Tsukuba T, Sakai H, Yamada M, Maeda H, Hori H, Azuma T, Akamine A, and Yamamoto K., Biochemical Properties of the Monomeric Mutant of Human Cathepsin E Expressed in Chinese Hamster Ovary Cells: Comparison with Dimeric Forms of the Natural and Recombinant Cathepsin E., J Biochemistry, 119, 1, 126-134, 119 (1): 126-134, 1996.01.
61. Kukita T, Kukita A, Nagata K, Maeda H, Kurisu K, Watanabe T, and Iijima T, Novel Cell-Surface Ag Expressed on Rat Osteoclasts Regulating the Function of the Calcitonin Receptor., J Immunology, 153, 11, 5265-5273, 153 (11): 5265-5273, 1995.01.
62. Maeda H, Kukita T, Akamine A, Kukita A, and Iijima T, Localization of Osteopontin in Resorption Lacunae Formed by Osteoclast-like Cells: A Study by a Novel Monoclonal Antibody which Recognizes Rat Osteopontin., Histochemistry, 10.1007/BF00269160, 102, 4, 247-254, 102 (4): 247-254, 1994.01.
Presentations
1. Aoi Nozu, Sayuri Hamano, Atsushi Tomokiyo, Daigaku Hasegawa, Shinichiro Yoshida, Hideki Sugii, Hiromi Mitarai, Keita Ipposhi, Naohisa Wada, Hidefumi Maeda., Effects of TNF-alpha on Senescent human dental pulp cells., Kyudai Oral Bioscience & OBT Research Center Joint International Symposium 2019, 2019.03.
2. Fujino S, Hamano S, Tomokiyo A, Hasegawa D, Yoshida Y, Sugii H, Washio A, Mitarai H, Nozu A, Arima M, Wada N, KitamuraC, Maeda H, Effects of dopamine on odontoblastic differentiation. , The IFEA 11th World Endodontic Congress 2018, 2018.10.
3. Arima M, Hasegawa D, Yoshida S, Mitarai H, Tomokiyo A, Hamano S, Sugii H, Wada N, Maeda H. , R-spondin2 Enhances Osteoblastic Differentiation of Immature Human Periodontal Ligament Cells., The 96th General Session & Exhibition of the IADR., 2018.07.
4. Tomokiyo A, Hamano S, Hasegawa D, Sugii H, Yoshida S, Mitarai H, Sonoda M, Nozu A, Wada N, Maeda H., Discoloration of White Mineral Trioxide Aggregate Immersed in Various Solutions., The 96th General Session & Exhibition of the IADR., 2018.07.
5. Sugii H, Grimaldi A, Li J, Parada C, Ho T-V, Feng J, Jing J, Yuan Y, Guo Y, Maeda H, Chai Y., Dlx5 Plays a Critical Role During Soft Palate Muscle Development., The 96th General Session & Exhibition of the IADR., 2018.07.
6. Nozu A, Hamano S, Tomokiyo A, Hasegawa D, Sugii H, Yoshida S, Mitarai H, Taniguchi S, Wada N, Maeda H., Odontoblastic differentiation of senescence dental pulp cells treated by TNF-α., The 96th General Session & Exhibition of the IADR., 2018.07.
7. Arima M, Hasegawa D, Yoshida S, Mitarai H, Tomokiyo A, Hamano S, Sugii H, Wada N, Maeda H., R-spondin2 enhances osteogenesis of immature human periodontal ligament cells through the canonical Wnt signaling pathway., Kyudai Oral Bioscience 2018, 2018.02.
8. Hidefumi Maeda, Differentiation and Aging of Pulp Cells., The 19th Scientific Congress of Asia Pacific Endodontic Confederation and 18th IACDE & IES PG Convention, 2017, 2017.04.
9. Hiromi Mitarai, Naohisa Wada, Daigaku Hasegawa, Shinichiro Yoshida, Mai Arima, Atsushi Tomokiyo, Sayuri Hamano, Hidefumi Maeda, Transgelin mediates TGF-beta1-induced human periodontal ligament cell proliferation., 95th General Session & Exhibition of the IADR, 2017.03.
10. Hiromi Mitarai, Naohisa Wada, Daigaku Hasegawa, Shinichiro Yoshida, Mai Arima, Atsushi Tomokiyo, Sayuri Hamano, Suguru Serita, Hiroyuki Mizumachi, Hidefumi Maeda, Transgelin mediates the proliferation of human periodontal ligament cells induced by TGF-β1., Kyudai Oral Bioscience 2017, 2017.02.
11. Shinichiro Yoshida, Naohisa Wada, Daigaku Hasegawa, Atsushi Tomokiyo, Sayuri Hamano, Hiromi Mitarai, Hideki Sugii, Hidefumi Maeda, Semaphorin 3A Induces Odontoblastic Phenotype in Dental Pulp Stem Cells., 94th General Session & Exhibition of the IADR., 2016.06.
12. Daigaku Hasegawa, Naohisa Wada, Sayuri Hamano, Atsushi Tomokiyo, Shinichiro Yoshida, Hiromi Mitarai, Mai Sonoda, Hideki Sugii, Hidefumi Maeda, Identification of a Novel Periodontal Ligament Stem Cell Marker., 94th General Session & Exhibition of the IADR., 2016.06.
13. Sayuri Hamano, Atsushi Tomokiyo, Naohisa Wada, Daigaku Hasegawa, Hideki Sugii, Shinichiro Yoshida, Suguru Serita, Hiroyuki Mizumachi, Hiromi Mitarai, Hidefumi Maeda, Directing Human iPS Cells Toward PDL Stem Cells., 94th General Session & Exhibition of the IADR., 2016.06.
14. Hideki Sugii, Atsushi Tomokiyo, Sayuri Hamano, Daigaku Hasegawa, 吉田 晋一郎, Suguru Serita, Hiroyuki Mizumachi, Hiromi Mitarai, Aoi Nozu, Marina Ogawa, Mai Sonoda, Naohisa Wada, Hidefumi Maeda, Activin A reversely works between human pre-osteoblastic cells and periodontal ligament cells on their osteoblastic differentiation. , 2nd symposium of Program for Advancing Strategic International Networks to Accelerate the Circulation of Talented Researchers, 2016.02.
15. Hidefumi Maeda, How can we save a severely-damaged tooth?, Kyudai Oral Bioscience 2016, 2016.02.
16. 吉田 晋一郎, Naohisa Wada, Daigaku Hasegawa, Atsushi Tomokiyo, Sayuri Hamano, Hiromi Mitarai, Suguru Serita, Hiroyuki Mizumachi, Hidefumi Maeda, Semaphorin 3A induces odontoblastic phenotype in dental pulp stem cells., Kyudai Oral Bioscience 2016, 2016.02.
17. Glial cell line-derived neurotrophic factor (GDNF) from human periodontal ligament cells treated with proinflammatory cytokines promotes neurocytic differentiation of PC12 cells..
18. The contribution of Calcium-sensing receptor on mineralization of human dental pulp cells..
19. Fluid analysis of laser-activated irrigation in apical ramification of the simulated canal model..
20. Transgelin is involved in the expression of alpha-SMA in periodontal ligament cells..
21. Generation of neural crest like-cells from human periodontal ligament- and foreskin-derived induce pluripotent stem cells..
22. Myna N. Zakaria, Toru Takeshita, Michiko Furuta, Hidefumi Maeda, Akifumi Akamine, Yoshihisa Yamashita, Oral Mycobiome in Japanese Elderly Adults., 93rd General Session & Exhibition of the IADR. , 2015.03.
23. Daigaku Hasegwa, Naohisa Wada, Hidefumi Maeda, Shinichiro Yoshida, Hiromi Mitarai, Atsushi Tomokiyo, Monnouchi Satoshi, Sayuri Hamano, Asuka Yuda, Akifumi Akamine, The Effects of Wnt5a on Human Periodontal Ligament Cells., 93rd General Session & Exhibition of the IADR., 2015.03.
24. Sayuri Hamano, Hidefumi Maeda, Daigaku Hasegawa, Monnouchi Satoshi, Naohisa Wada, Atsushi Tomokiyo, Asuka Yuda, Hideki Sugii, Shinichiro Yoshida, Akifumi Akamine, Effect of β2 Adrenergic Receptor on Human Periodontal Ligament Cells. , 93rd General Session & Exhibition of the IADR., 2015.03.
25. Asuka Yuda, Hidefumi Maeda, Fujii Shinsuke, Monnouchi Satoshi, Naohisa Wada, Atsushi Tomokiyo, Sayuri Hamano, Daigaku Hasegawa, Hideki Sugii, Shinichiro Yoshida, Akifumi Akamine, Collaborative Activity of CTGF/CCN2 and TGF-β1 on Osteogenesis and Fibrogenesis., 93rd General Session & Exhibition of the IADR., 2015.03.
26. Daigaku Hasegawa, naohisa wada, Hidefumi Maeda, Shinichiro Yoshida, Monnouchi Satoshi, Koori Katsuaki, Sayuri Hamano, Hiromi Mitarai, Akifumi Akamine, The Effects of Wnt5a on Human Periodontal Ligament Cells. , Kyudai Oral Bioscience 2014 —8th International Symposium —, 2014.02.
27. Hidefumi Maeda, To seek a longer conservation method of tooth, 1st International Conference on Dental & Oral Health, 2013.08, In Japan, we meet the aging of society, and along with this, people desire to retain their teeth as long as possible. Periodontitis, caries, fracture, and orthodontic issue are known to be as major causes of tooth loss. To correct these problems, clinicians and researchers have made efforts to develop novel and unique therapies. In this context, we have focused on treatment for caries and fracture. To address this issue, we have been attempting to clarify the mechanism of the regeneration of periodontal ligament (PDL) tissue and to develop functional filling materials to permit this event. For the former agenda, we have investigated to define PDL stem cells, signaling and scaffolds based on tissue engineering standpoint. Firstly we have established and characterized two undifferentiated human PDL clonal cell lines that had been immortalized by gene transfer, and then examined the effects of various cytokines and calcium on these cell lines. For the latter agenda, we have prepared calcium-modified 4-META/MMA-TBB resin, and evaluated its bioactive features, such as biocompatibility, formation of hydroxyapatite on its surface, and differentiation-inducing activity for human PDL cells. Thus to elucidate above-described three elements optimal to allow periodontal regeneration, and to develop functional filling materials with characteristics as a scaffold for that will lead us to pioneer a new dental therapy for longer conservation of tooth. This technique may be also prospective in further application for developing the implant retaining PDL tissue or a treatment of periodontitis..
28. ZAKARIA MN, Hidefumi Maeda, naohisa wada, Akifumi Akamine, Molecular Assessment of Bacterial Community in Persistent Apical Periodontitis Granulomatous Tissue: 16S rDNA Clone Library Analysis. , The 9th World Endodontic Congress of IFEA., 2013.05.
29. 吉田晋一郎, naohisa wada, 前田 英史, 長谷川大学, 佐藤浩美, Monnouchi Satoshi, Akifumi Akamine, Dental Pulp Stem Cells on Bone Tissue Express Periodontal Ligament-related gene., The 9th World Endodontic Congress of IFEA., 2013.05.
30. 門野内 聡, 前田 英史, 祐田明香, 赤峰 昭文, Localization of βig-h3 and Collagen Type I in Dental Pulp., The 9th World Endodontic Congress of IFEA., 2013.05.
31. 前田 英史, 郡 勝明, 和田 尚久, 友清 淳, 門野内 聡, 山本直秀, 赤峰 昭文, Evaluation of calcium-modified 4-META/MMA-TBB resin on its bioactive feature. , The 9th World Endodontic Congress of IFEA., 2013.05.
32. 郡勝明, 前田 英史, 和田 尚久, 友清 淳, 門野内 聡, 山本直秀, 赤峰 昭文, Extracellular Calcium Regulates Osteoblastic Differentiation of Undifferentiated PDL Cells., 91st General Session & Exhibition of the IADR, 2013.03.
33. 山本直秀, 前田 英史, 友清 淳, 和田 尚久, 門野内 聡, 郡勝明, 赤峰 昭文, Roles of Glial Cell-Derived Neurotrophic Factor in Periodontal Ligament Cells., 91st General Session & Exhibition of the IADR, 2013.03.
34. 和田 尚久, 前田 英史, 長谷川大学, S. GRONTHOS, P.M. BARTOLD, D. MENICANIN, 藤井 慎介, Hiroko Wada, Atsushi Tomokiyo, Monnouchi Satoshi, Akifumi Akamine, Conversion of periodontal ligament cells into multipotent stem-like cells., 91st General Session & Exhibition of the IADR., 2013.03.
35. 前田 英史, 友清 淳, 郡勝明, 山本直秀, 和田 尚久, 門野内 聡, 濱野さゆり, 祐田明香, 赤峰 昭文, CaCl2-added Resin Exerts Bioactive Effects on Periodontal Ligament Cells. , 91st General Session & Exhibition of the IADR., 2013.03.
36. 和田 尚久, 前田 英史, 長谷川大学, Gronthos S, Bartold PM, Menicanin D, 藤井 慎介, 和田 裕子, 友清 淳, 門野内 聡, 赤峰 昭文, Induction of stem/undifferentiated cells from periodontal ligament cells by Sema3A. , The Australian Health and Medical Research Congress 2012, 2012.11.
37. The effects of Dioxins on osteoblastic differentiation of human periodontal ligament cells.
38. The effects of Sema3A on stemness induction of human periodontal ligament cells.
39. Exposure to transforming growth factor-beta1 after basic fibroblast growth factor promotes the fibroblastic differentiation of human periodontal ligament stem/progenitor cell lines. .
40. Potency of Mechanical Load in Differentiation of Periodontal Ligament Stem Cells..
41. Stretched periodontal ligament cells up-regulate Interleukin-11 to regenerate osteoblastic metabolism..
42. The effects of passive ultrasonic irrigation on root irrigation -SEM observation-.
43. Stretch loading induces the expression of interleukin-11 in human periodontal ligament cells..
44. Resin-based sealers exhibit high sealing ability; thus, they are useful for endodontic
treatment. Some reports concerning cytotoxicity of the eluent from these sealers,
however, have been presented. The purpose of this study was to evaluate the effects
of a polymethyl methacrylate resin-based sealer (Superbond Sealer™, SB) on
proliferation and osteogenic differentiation of human periodontal ligament cells
(HPLCs) in vitro, compared with an epoxy resin-based sealer (AH Plus™, AH). HPLCs
were obtained from two patients with informed consent. SB or AH discs washed for 7
days after setting (washed) or unwashed discs just after setting for 24 hr (unwashed)
were prepared. Cells cultured on these discs were evaluated by scanning electron
microscopy (SEM) and WST-1 proliferation assays. The osteogenic differentiation
of HPLCs cultured on these discs was examined by gene expression analysis of
osteopontin (OPN) and osteocalcin (OCN) by quantitative RT-PCR. HPLCs exhibited
growth on washed SB discs, whereas unwashed SB and AH discs and washed AH discs
inhibited their proliferation. SEM observation revealed that HPLCs tightly attached
onto the surface of washed SB discs, although few cells were observed on other discs.
HPLCs significantly upregulated gene expression of OPN and OCN when cultured
on washed SB discs in osteogenic differentiation medium for 2 weeks whereas other
discs did not induce their differentiation. These results indicate that, although SB
initially exerted cytotoxic effects on HPLCs, these effects reduced during washing for
7 days compared to AH. Washed SB might allow HPLCs to differentiate into osteogenic
cells under the osteogenic environment. This study was supported by the Ministry of
Education, Culture, Sports, Science and Technology in Japan..
45. The roles of stretch loading in human periodontal ligament cells..
46. Contribution of mechanical load to the regeneration of human periodontal ligament tissues..
47. The effects of calcium on differentiation of human periodontal ligament stem/progenitor cell lines..
48. The effects of basic fibroblast growth factor on the fibroblastic differentiation of human undifferentiated periodontal ligamen cell lines..
49. The effects of glial cell-derived neurotrophic factor on chemotaxis of human periodontal ligament cells..
50. Immunomodulatory properties of human periodontal ligament cells and dental pulp cells..
51. Characteristics of Superbond sealer –Its effects on proliferation and differentiation of human periodontal ligament cells-
.
52. The roles of angiotensin II in stretched periodontal ligament cells. .
53. Fibroblastic cells from human periapical granulation tissue preferentially form calcified matrices in boiled decalcified rat bone..
54. Angiotensin II mediates the loading signal in human periodontal ligament cells..
55. What are the characteristics of PDL stem cells?.
56. Angiotensin II is involved in the loading signal in stretched human PDL cells..
57. What characteristics do PDL stem cells have?.
58. MTA stimulates BMP2 expression in human PDL cells. .
59. Calcium-releasing Agent Exhibits Bioactive Effects in Endodontic Therapy..
60. A Multipotent hPDL Cell Line Promotes Neurocytic Differentiation and Migration..
61. MTA induces mineralization of human periodontal ligament cells..
Membership in Academic Society
  • Japanese Dental Education Association
  • Japan Association of Microscopic Dentistry
  • American Association of Endodontists
  • Japan Society for Regenerative Medicine
Awards
  • The effect of TNF-α on the aging and odontoblast-like differentiation of human dental pulp cells.
  • Generation of neural crest like-cells from human periodontal ligament- and foreskin-derived induced pluripotent stem cells.
  • Effect of agonist of antagonist of beta2 adrenergic receptor on human periodontal ligament cells.
  • Evaluation of the regenerative mechanism of periodontal ligament tissue
  • Biocompatibility of resin-based sealers- Comparison of Superbond sealer with AH Plus -
  • Characteristics of Superbond sealer –Its effects on proliferation and differentiation of human periodontal ligament cells-
  • The effects of TGF-β1 on proliferation and differentiation of human PDL fibroblasts and a human PDL stem/ progenitor cell line.
  • Effects of root-end filling materials on the osteoblast-like differentiation of human periodontal ligament cells
  • Influence of EMD and bFGF on mineralization in human periodontal ligament cell lines.
Educational
Other Educational Activities
  • 2019.02.
  • 2017.12.
  • 2011.05.
  • 2011.03.
  • 2010.08.
  • 2009.11.
  • 2008.11.
  • 2007.11.
  • 2006.01.
  • 2005.01.
  • 2004.01.
  • 2003.01.
  • 2002.01.
  • 2001.01.
  • 2000.01.