Kyushu University Academic Staff Educational and Research Activities Database
List of Papers
Yasunori AYUKAWA Last modified date:2019.07.05

Associate Professor / Division of Oral Rehabilitation / Department of Dental Science / Faculty of Dental Science


Papers
1. Yoko Takemura, Yasuko Moriyama, Yasunori Ayukawa, Kosaku Kurata, Yunia D. Rakhmatia, Kiyoshi Koyano, Mechanical loading induced osteocyte apoptosis and connexin 43 expression in three-dimensional cell culture and dental implant model, Journal of Biomedical Materials Research - Part A, 10.1002/jbm.a.36597, 107, 4, 815-827, 2019.04, Osteocytes are thought to act as stress sensors, and are known to display a gap junction-mediated stress-transfer mechanism. To demonstrate the stress-related function of osteocytes, cells of an osteocyte-like cell line derived from murine long bone osteocyte Y4 (MLO-Y4) were cultivated in a three-dimensional culture and subjected to cyclic loading from a titanium plate. This application of physiological loading using a titanium plate significantly increased connexin 43 (Cx43) expression, the number of dead and apoptotic cells, and receptor activator of nuclear factor κB ligand expression. Furthermore, the conditioned medium from the loaded osteocytes induced alkaline phosphatase activity in bone marrow cell culture. In addition, we immunohistologically determined whether bone metabolism increased as a result of the occlusal force in the bone surrounding the titanium implants in a rat model. Increased Cx43 expression and apoptotic osteocytes were observed in the loading group as well as a significantly increased number of tartrate-resistant acid phosphatase-positive cells. These findings indicate that stress from the implant adversely affected the osteocytes, which may promote osteoclastic and osteoblastic cell formation around the implants..
2. Mikio Imai, Yasunori Ayukawa, Noriyuki Yasunami, Akihiro Furuhashi, Yoko Takemura, Naomi Adachi, Jiangqi Hu, Xudiyang Zhou, Yasuko Moriyama, Atsuta Ikiru, Kosaku Kurata, Kiyoshi Koyano, Effect of a Single Injection of Benidipine-Impregnated Biodegradable Microcarriers on Bone and Gingival Healing at the Tooth Extraction Socket, Advances in Wound Care, 10.1089/wound.2018.0834, 8, 3, 108-117, 2019.03, Objective: A dihydropyridine-type calcium channel blocker, benidipine (BD), is extensively used in hypertension therapy. In vitro study reported BD promoting bone metabolism. We evaluated the effect of sustained release of BD-loaded poly(lactic-co-glycolic acid) (PLGA) microcarriers on the promotion of bone and gingival healing at an extraction socket in vivo. In addition, the effect of BD on osteoblasts, osteocytes, fibroblasts, and epithelial cells was evaluated in vitro. Approach: The maxillary first molar of rats was extracted. Next, PLGA microcarriers containing BD were directly injected into the gingivobuccal fold as a single dose. After injection, bone and soft-tissue healing was histologically evaluated. Effect of BD on proliferation, migration, and gene expression of gingival and bone cell was also examined in vitro. Results: After tooth extraction, BD significantly augmented bone volume and density, and also epithelial wound healing. During in vitro studies, BD promoted significant proliferation and migration of fibroblasts and epithelial cells. Real-time RT-PCR revealed that BD upregulated messenger RNA expression of Ahsg (alpha 2-HS glycoprotein) and Csf2 (colony-stimulating factor 2) in osteoblasts. Innovation: The prevention of bone and soft-tissue reduction associated with tooth extraction has been eagerly anticipated in the field of dentistry. This study first reported the effect of BD on extraction socket healing. Conclusion: A single dose of topically administered BD-loaded PLGA microcarriers promoted bone and soft-tissue healing at the extraction site of tooth..
3. Haomiao Zhang, Yasuko Moriyama, Yasunori Ayukawa, Yunia Dwi Rakhmatia, Yoko Takemura, Noriyuki Yasunami, Kiyoshi Koyano, Generation and histomorphometric evaluation of a novel fluvastatin-containing poly(lactic-co-glycolic acid) membrane for guided bone regeneration, Odontology, 10.1007/s10266-018-0376-z, 107, 1, 37-45, 2019.01, The purpose of this study was to evaluate the effects of a poly(lactic-co-glycolic acid) (PLGA) membrane containing fluvastatin on bone regeneration at bone defects in rat calvaria and tibia for possible use as a guided bone regeneration (GBR) membrane. PLGA and fluvastatin-containing PLGA (PLGA–fluvastatin) membranes were prepared and mechanical properties were evaluated. Standardized bony defects were created in rat calvaria and the right tibia, and covered with a PLGA or PLGA–fluvastatin membrane. Bone regeneration was evaluated using image analysis based on histologic examination. At 4 and 8 weeks after membrane implantation, the PLGA–fluvastatin group displayed enhanced new bone formation around the edge of the defect compared with the PLGA membrane group in the calvarial model. Thick bone regeneration was observed in tibia-defect sites in the PLGA–fluvastatin membrane group. These results suggest that the PLGA-containing fluvastatin membrane prepared in this study may potentially be used as a GBR membrane..
4. Atsuta Ikiru, Yasunori Ayukawa, Akihiro Furuhashi, Ikue Narimatsu, Ryosuke Kondo, Wakana Oshiro, Kiyoshi Koyano, Epithelial sealing effectiveness against titanium or zirconia implants surface, Journal of Biomedical Materials Research - Part A, 10.1002/jbm.a.36651, 2019.01, The aims of implant treatment now involve not only restoration of mastication function, but also recovery of esthetics. Currently, zirconia is widely used as an esthetic material for implant abutment. Therefore, it is very important to understand the efficacy of zirconia for epithelial sealing as an implant material. We compared the effects of materials on the sealing of the peri-implant epithelium (PIE) to titanium (Ti) or zirconia (Zr) implants, for application to clinical work. Maxillary first molars were extracted from rats and replaced with Ti or Zr implants. The sealing of the PIE to the implants was evaluated with immunohistochemistry observation and HRP analysis. The morphological and functional changes in rat oral epithelial cells (OECs) cultured on Ti or Zr plates were also evaluated. After 4 weeks, the PIE on the Ti and Zr implants showed similar structures. The Zr implants appeared to form a weak epithelial seal at the tissue–implant interface, and exhibited markedly less adhesive structures than the Ti implants under electron microscopic observation. In the in vitro experiments, decreased expression levels of adhesion proteins were observed in OECs cultured on Zr plates compared with those cultured on Ti plates. In addition, the cell adherence on Zr plates was reduced, while the cell migration was low on Ti plates. Zr is a better choice for an esthetic implant material, but needs further improvement for integration with the epithelial wound healing process around a dental implant..
5. Yunia Dwi Rakhmatia, Yasunori Ayukawa, Akihiro Furuhashi, Kiyoshi Koyano, Carbonate apatite containing statin enhances bone formation in healing incisal extraction sockets in rats, Materials, 10.3390/ma11071201, 11, 7, 2018.07, The purpose of this study was to evaluate the feasibility of using apatite blocks fabricated by a dissolution-precipitation reaction of preset gypsum, with or without statin, to enhance bone formation during socket healing after tooth extraction. Preset gypsum blocks were immersed in a Na3PO4 aqueous solution to make hydroxyapatite (HA) low crystalline and HA containing statin (HAFS), or in a mixed solution of Na2HPO4 and NaHCO3 to make carbonate apatite (CO) and CO containing statin (COFS). The right mandibular incisors of four-week-old male Wistar rats were extracted and the sockets were filled with one of the bone substitutes or left untreated as a control (C). The animals were sacrificed at two and four weeks. Areas in the healing socket were evaluated by micro-computed tomography (micro-CT) and histological analyses. The bone volume, trabecular thickness, and trabecular separation were greatest in the COFS group, followed by the CO, HAFS, HA, and C groups. The bone mineral density of the COFS group was greater than that of the other groups when evaluated in the vertical plane. The results of this study suggest that COFS not only allowed, but also promoted, bone healing in the socket. This finding could be applicable for alveolar bone preservation after tooth extraction..
6. Tsuruta K, Ayukawa Y, Matsuzaki T, Kihara M, Koyano K., The influence of implant—abutment connection on the screw loosening and microleakage , International Journal of Implant Dentistry, 4, 11, 2018.06.
7. Mieda M, Atsuta I, Morita T, Ayukawa Y, Tsukiyama Y, Sawae Y, Koyano K. , The effective design of zirconia coping on titanium base in dental implant superstructure., Dent Mater J, 2018.06.
8. Zhang H, Moriyama Y, Ayukawa Y, Rakhmatia YD, Tomita Y, Yasunami N, Koyano K., Generation and histomorphometric evaluation of a novel fluvastatin-containing poly(lactic-co-glycolic acid) membrane for guided bone regeneration, Odontology, 2018.06.
9. Mariko Urata, Shoichiro Kokabu, Takuma Matsubara, Goro Sugiyama, Chihiro Nakatomi, Hiroshi Takeuchi, Shizu Hirata-Tsuchiya, Kazuhiro Aoki, Yukihiko Tamura, Yasuko Moriyama, Yasunori Ayukawa, Miho Matsuda, Min Zhang, Kiyoshi Koyano, Chiaki Kitamura, Eijiro Jimi, A peptide that blocks the interaction of NF-κB p65 subunit with Smad4 enhances BMP2-induced osteogenesis, Journal of Cellular Physiology, 10.1002/jcp.26571, 233, 7356-7366, 2018.01.
10. Tomoko Nishimura, Yoichiro Ogino, Yasunori Ayukawa, Kiyoshi Koyano, Influence of the wettability of different titanium surface topographies on initial cellular behavior, dental materials journal, 10.4012/dmj.2017-334, 37, 4, 650-658, 2018.01, This study examined the influence of the time-dependent wettability of different surface topographies on initial cellular behavior. Titanium disks with smooth topography (SM) and three kinds of rough topography (sandblasted (SA), microtopography (M) and nanotopography (N)) were prepared. Time-dependent changes in surface wettability were observed in all surfaces as shown in previous studies. On SM surfaces, hydrophobic alteration influenced cell spreading and the activity of RhoA (a small GTPase protein of the Rho family), while no alterations were observed on rough surfaces except for the number of adherent cells. Serum adsorption could recover these functional deteriorations by hydrophobic alteration. These findings suggest that surface topography is a more potent regulator in initial cellular behaviors such as cell spreading and RhoA activation than surface wettability..
11. Kanazawa M,Atsuta I,Ayukawa Y,Yamaza T,Kondo R,Matsuura Y,Koyano K, The influence of systemically or locally administered mesenchymal stem cells on tissue repair in a rat oral implantation model, International Journal of Implant Dentistry, 4, 2, 2018.01.
12. Norio Goto, Ken Okazaki, Yukio Akasaki, Kohei Ishihara, Koji Murakami, Kiyoshi Koyano, Yasunori Ayukawa, Noriyuki Yasunami, Tomohiro Masuzaki, Yasuharu Nakashima, Single intra-articular injection of fluvastatin-PLGA microspheres reduces cartilage degradation in rabbits with experimental osteoarthritis, Journal of Orthopaedic Research, 10.1002/jor.23562, 35, 11, 2465-2475, 2017.11, Statins are cholesterol-lowering drugs that inhibit 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase, a rate-limiting enzyme of the mevalonate pathway. The anti-inflammatory effect of statins has been reported in recent years. The present study investigated therapeutic effects of the local administration of statin in osteoarthritis (OA). We assessed clinically used statins and selected fluvastatin for further experimentation, as it showed potent anabolic and anti-catabolic effects on human OA chondrocytes. To achieve controlled intra-articular administration of statin, we developed an intra-articular injectable statin using poly(lactic-co-glycolic acid) (PLGA) as a drug delivery system (DDS). The release profile of the statin was evaluated in vitro. Finally, therapeutic effects of fluvastatin-loaded PLGA microspheres (FLU-PLGA) were tested in a rabbit OA model. Rabbit knees were divided into four subgroups: group 1-A, PLGA-treated group; group 1-B, PLGA contralateral saline control group; group 2-A, FLU-PLGA-treated group; and group 2-B, FLU-PLGA contralateral saline control group. Histological analysis 5 weeks after intra-articular injection revealed that OARSI scores were lower in group 2-A. No significant differences in OARSI scores were observed between groups 1-A, 1-B, and 2-B. This study indicates that a single intra-articular injection of fluvastatin-loaded PLGA microspheres could be a novel therapeutic approach for treating patients with OA..
13. Yunia Dwi Rakhmatia, Yasunori Ayukawa, Yohei Jinno, Akihiro Furuhashi, Kiyoshi Koyano, Micro-computed tomography analysis of early stage bone healing using micro-porous titanium mesh for guided bone regeneration
preliminary experiment in a canine model, Odontology, 10.1007/s10266-017-0298-1, 105, 4, 408-417, 2017.10, The aim of this study was to evaluate the amount of bone formation beneath a defect area after treatment with titanium mesh membranes with different thicknesses and pore sizes alone or in combination with bone graft to induce bone formation during the early stage of healing time. The mandibular premolars were extracted bilaterally from three adult beagle dogs, and 8-mm-diameter bone defects were created on the buccal site of the premolar regions. Hydroxyapatite bone graft substitute was applied in the defect site unilaterally, and other site was left empty. Then, a novel micro-porous mesh (50 μm in pore diameter) or commercially available macro-porous titanium mesh (1700 μm in pore diameter) was placed on the defect and secured with screws. After 4 weeks, the mandibles were harvested, imaged using micro-computed tomography, and prepared for histological and morphometric evaluation. Higher new bone volumes (mm3), percentage of new bone volumes in the total defect volumes (bone ratio: %), and new bone area (mm2) through morphometric evaluation were found on the novel membranes with 50-μm-diameter pores compared to the commercial titanium mesh. Moreover, experiment sites without bone graft were observed with higher new bone volume and bone ratio compared with sites with bone graft. However, bone mineral density of novel mesh was observed to be lower compared with other experimental sites. Under the experimental condition, the result of this study suggests that titanium meshes with 50-μm-diameter pores were effective for guided bone regeneration in the early stage of healing..
14. Hideo Shimizu, Yohei Jinno, Yasunori Ayukawa, Ikiru Atsuta, Takaaki Arahira, Mitsugu Todo, Kiyoshi Koyano, Tissue reaction to a novel bone substitute material fabricated with biodegradable polymer-calcium phosphate nanoparticle composite, Implant Dentistry, in press, 2016.10, Purpose: The aim of this study was to evaluate the effectiveness of a novel bone substitute material fabricated using a biodegradable polymer-calcium phosphate nanoparticle composite. Methods: Porous structured poly-l-lactic acid (PLLA) and hydroxyapatite (HA) nanoparticle composite, which was fabricated using solid-liquid phase separation and freeze-drying methods, was grafted into bone defects created in rat calvarium or tibia. Rats were killed 4 weeks after surgery, and histological analyses were performed to evaluate new bone formation. Results: Scanning electron microscopic observation showed the interconnecting pores within the material and the pore diameter was approximately 100 to 300 m. HA nanoparticles were observed to be embedded into the PLLA beams. In the calvarial implantation model, abundant blood vessels and fibroblastic cells were observed penetrating into pores, and in the tibia model, newly formed bone was present around and within the composite. Conclusions: The PLLA-HA nanoparticle composite bone substitute developed in this study showed biocompatibility, elasticity, and operability and thus has potential as a novel bone substitute..
15. Shinichiro Kuroshima, Masaru Kaku, Takashi Matsuura, Ikiru Atsuta, Yasunori Ayukawa, Takashi Sawase, Medication-Related Osteonecrosis of the Jaw; What Should We Do as Prosthodontists?, Journal of Prosthodontic Research, in press, 2016.10.
16. Matsuzaki M, Ayukawa Y, Sakai N, Matsuzaki T, Yasuyuki Matsushita, Koyano K, A comparison of the peri-implant bone stress generated by the preload with screw tightening between 'bonded' and 'contact' model., Computer methods in biomechanics and biomedical engineering, 20, 393-402, 2016.09.
17. Yuri Matsuura, Atsuta Ikiru, Yasunori Ayukawa, Takayoshi Yamaza, Ryosuke Kondo, akira takahashi, Nobuyuki Ueda, Wakana Oshiro, Yoshihiro Tsukiyama, Kiyoshi Koyano, Therapeutic interactions between mesenchymal stem cells for healing medication-related osteonecrosis of the jaw, Stem Cell Research and Therapy, 10.1186/s13287-016-0367-3, 7, 1, 2016.08, Background: Mesenchymal stem cells (MSCs) have been isolated from a variety of tissues, including bone marrow, adipose, and mucosa. MSCs have the capacity for self-renewal and differentiation. Reports have been published on the systemic administration of MSCs leading to functional improvements by engraftment and differentiation, thus providing a new strategy to regenerate damaged tissues. Recently, it has become clear that MSCs possess immunomodulatory properties and can therefore be used to treat diseases. However, the therapeutic effect mechanisms of MSCs are yet to be determined. Here, we investigated these mechanisms using a medication-related osteonecrosis of the jaw (MRONJ)-like mouse model. Methods: To generate MRONJ-like characteristics, mice received intravenous zoledronate and dexamethasone two times a week. At 1 week after intravenous injection, maxillary first molars were extracted, and at 1 week after tooth extraction, MSCs were isolated from the bone marrow of the mice femurs and tibias. To compare "diseased MSCs" from MRONJ-like mice (d-MSCs) with "control MSCs" from untreated mice (c-MSCs), the isolated MSCs were analyzed by differentiation and colony-forming unit-fibroblast (CFU-F) assays and systemic transplantation of either d-MSCs or c-MSCs into MRONJ-like mice. Furthermore, we observed the exchange of cell contents among d-MSCs and c-MSCs during coculture with all combinations of each MSC type. Results: d-MSCs were inferior to c-MSCs in differentiation and CFU-F assays. Moreover, the d-MSC-treated group did not show earlier healing in MRONJ-like mice. In cocultures with any combination, MSC pairs formed cell-cell contacts and exchanged cell contents. Interestingly, the exchange among c-MSCs and d-MSCs was more frequently observed than other pairs, and d-MSCs were distinguishable from c-MSCs. Conclusions: The interaction of c-MSCs and d-MSCs, including exchange of cell contents, contributes to the treatment potential of d-MSCs. This cellular behavior might be one therapeutic mechanism used by MSCs for MRONJ..
18. Noriyuki Yasunami, Yasunori Ayukawa, Akihiro Furuhashi, Ikiru Atsuta, Rakhmatia Yunia D, Yasuko Moriyama, Masuzaki Tomohiro, Kiyoshi Koyano, Acceleration of hard and soft tissue healing in the oral cavity by a single transmucosal injection of fluvastatin-impregnated poly (lactic-co-glycolic acid) microspheres. An in vitro and rodent in vivo study., Biomedical Materials, 11, 1, 015001, 2015.12.
19. Hideaki Tanaka, Takeshi Toyoshima, Ikiru Atsuta, Yasunori Ayukawa, Masanori Sasaki, Yasuyuki Matsushita, Ryu Hiraoka, Kiyoshi Koyano, Seiji Nakamura, Additional effects of platelet rich fibrin on bone regeneration in sinus augmentation with deproteinized bovine bone mineral: preliminary results , Implant Dentistry, 24, 6, 669-674, 2015.12.
20. Takeshi Toyoshima, Hideaki Tanaka, Yasunori Ayukawa, Miori Howashi, Masuzaki Tomohiro, Takahiro Kiyosue, Kiyoshi Koyano, Seiji Nakamura, Primary Stability of a Hybrid Implant Compared with Tapered and Cylindrical Implants in an Ex Vivo Model, Clinical Implant Dentistry andRelated Research, 10.1111/cid.12205, 17, 5, 950-956, 2015.10.
21. Wakana Oshiro, Yasunori Ayukawa, Ikiru Atsuta, Akihiro Furuhashi, Junichi Yamazoe, Ryosuke Kondo, Mami Sakaguchi, Yuri Matsuura, Yoshihiro Tsukiyama, Kiyoshi Koyano, Promoting effect of CaCl2 hydrothermal treatment to titanium implant surface for early epithelial sealing., Colloids and Surfaces B: Biointerfaces, 131, 1, 141-147, 2015.10.
22. Yasunori Ayukawa, Yumiko Suzuki, Kanji Tsuru, Kiyoshi Koyano, Kunio Ishikawa, Histological comparison in rats between carbonate apatite fabricated from gypsum and sintered hydroxyapatite on bone remodeling., Biomed Research International, 579541, 2015.07.
23. Yunia Dwi Rakhmatia, Yasunori Ayukawa, Akihiro Furuhashi, Ikiru Atsuta, Kiyoshi Koyano, Fibroblast attachment onto novel titanium mesh membranes for guided bone regeneration, Odontology, 10.1007/s10266-014-0151-8, 103, 2, 218-226, 2015.05.
24. Hideaki Tanaka, Takeshi Toyoshima, Atsuta Ikiru, Yasunori Ayukawa, Masanori Sasaki, Yasuyuki Matsushita, Ryu Hiraoka, Kiyoshi Koyano, Seiji Nakamura, Additional effects of platelet-rich fibrin on bone regeneration in sinus augmentation with deproteinized bovine bone mineral
Preliminary results, Implant Dentistry, 10.1097/ID.0000000000000306, 24, 6, 669-674, 2015.01, Purpose: Platelet-rich fibrin (PRF) has been reported to contribute to bone regeneration; however, little is known about details with deproteinized bovine bone mineral (DBBM). This study aims to evaluate additional effects of PRF on bone regeneration in sinus augmentation with DBBM. Materials and Methods: Sinus augmentations were made with DBBM/PRF mixture as lateral approach for 4 sinuses from 4 patients. Bone biopsies were obtained from posterior maxilla at the implant placements 7 or 10 months after sinus augmentations. Histological observations and histomorphometric analyses from augmented areas were performed. Results: The new bone formation was found around the DBBMs with very good contact while surfaces of DBBMs were partly resorbed. Osteoclasts recognized the DBBMs for remodeling, followed by new bone running. The histomorphometric analyses revealed that mean percentages of newly formed bone were 31.7 ± 1.2%, 21.0 ± 1.0%, 38.0 ± 0.6%, and 47.0 ± 0.6%, respectively (mean 34.5 ± 5.7%). Conclusions: Additional effects of PRF could be found because of higher percentages of newly bone formation by DBBM/PRF mixture than those by DBBM individual in previous reports..
25. Yunia Dwi Rakhmatia, Yasunori Ayukawa, Akihiro Furuhashi, Kiyoshi Koyano, Micro-CT and histomorphometric analyses of novel titanium mesh membranes for guided bone regeneration: A study on rat calvarial defects, International Journal of Oral and Maxillofacial Implants, 2014.12.
26. Miori Howashi, Yoshihiro Tsukiyama, Yasunori Ayukawa, Kei Isoda-Akizuki, Masafumi Kihara, Yu Imai, Motofumi Sogo, Kiyoshi Koyano, Relationship between the CT Value and Cortical Bone Thickness at Implant Recipient Sites and Primary Implant Stability with Comparison of Different Implant Types, Clinical Implant Dentistry andRelated Research, 18, 1, 107-116, 2014.11.
27. Yoichiro Ogino, Yasunori Ayukawa, Yoko TOMITA, Kiyoshi Koyano, Prosthetic aspects in adult osteoporosis, Journal of Prosthetic Dentistry, 2014.10.
28. Ikiru Atsuta, Yasunori Ayukawa, Akihiro Furuhashi, Yoichiro Ogino, Moriyama Yasuko, Yoshihiro Tsukiyama, Kiyoshi Koyano, In vivo and in vitro studies of epithelial cell behavior around titanium implants with machined and rough surface, Clinical Implant Dentistry andRelated Research, 10.1111/cid.12043, 16, 5, 772-781, 2014.10.
29. Akinari Nakagawa, Naoki Shitara, Yasunori AYUKAWA, Kiyoshi Koyano, Kenji Nishimura, Implant treatment followed by living donor lung transplant: A follow-up case report, Journal of Prosthodontic Research, http://dx.doi.org/10.1016/j.jpor.2013.11.001, 58, 2, 127-131, 2014.04.
30. Toshio Takano, Yin-Ji Li, Akiko Kukita, Takayoshi Yamaza, Yasunori Ayukawa, Kanako Moriyama, Norihisa Uehara, Hisayuki Nomiyama, Kiyoshi Koyano, Toshio Kukita, Mesenchymal Stem Cells Markedly Suppress Inflammatory Bone Destruction in Rats with Adjuvant-Induced Arthritis, Laboratory Investigation, 94, 286-296, 2014.03.
31. Ryosuke Kondo, Ikiru Atsuta, Yasunori Ayukawa, Takayoshi Yamaza, Yuri Matsuura, Akihiro Furuhashi, Yoshihiro Tsukiyama, Kiyoshi Koyano, Therapeutic Interaction of Systemically-administered Mesenchymal Stem Cells with Peri-implant Mucosa, PLoS One, 10.1371/journal.pone.0090681, 9, 3, e90681, 2014.03.
32. Koichi Yamane, Yasunori Ayukawa, Toru Takeshita, Akihiro Furuhashi, Yoshihisa Yamashita, Kiyoshi Koyano, Bacterial adhesion affinities of various implant abutment materials, Clinical Oral Implants Research, 24, 1310-1315, 2013.12.
33. Ikiru Atsuta, Yasunori Ayukawa, Takayoshi Yamaza, Akihiro Furuhashi, Kiyoshi Koyano, The role of phosphoinositide 3-kinase in adhesion of oral epithelial cells to titanium, Archives of Oral Biology, 2013.11.
34. Ikiru Atsuta, Yasunori Ayukawa, Akihiro Furuhashi, Takayoshi Yamaza, Hideyuki Okwachi, Kiyoshi Koyano, Promotive effect of insulin-like growth factor-1 for epithelial sealing to titanium implants, Journal of Biomedical MAterials Research A, 101, 2896-2904, 2013.10.
35. Daisuke Kondo, YOICHIRO OGINO, Yasunori AYUKAWA, Shinji Sakai, KOEI KAWAKAMI, kiyoshi koyano, Bone regeneration of rat tibia defects with enzymatically hydrogelation of gelatin derivative and recombinant human platelet-derived growth factor-BB complex, Int J Oral Maxillofac Implants, 2013.08.
36. Akira Takahashi, Akiko Kukita, Li YinJi, Jing-qi Zhang, Hisayuki Nomiyama, Takayoshi Yamaza, Yasunori AYUKAWA, kiyoshi koyano, Toshio Kukita, Tunneling Nanotube Formation is Essential for the Regulation of Osteoclastogenesis, J Cellular Biochem, 114, 6, 1238-1247, 2013.06.
37. Akihiro Furuhashi, Yasunori AYUKAWA, ATSUTA IKIRU, Rakhmatia YD, Noriyuki Yasunami, kiyoshi koyano, Influence of titanium surface topography on peri-implant soft tissue integration, Key Engineering Materials, 529-530, 559-564, 2013.04.
38. ATSUTA IKIRU, Yasunori AYUKAWA, Takayoshi Yamaza, Akihiro Furuhashi, Ryosuke Kondo, kiyoshi koyano, Expression of Integrin alpha-3 and beta-4 subunits on the process of peri-implant epithelium formation, Key Engineering Materials, 529-530, 407-412, 2013.04.
39. Daisuke Esaki, Yasuyuki Matsushita, Yasunori AYUKAWA, NOBUO SAKAI, Yoshinori Sawae, kiyoshi koyano, Relationship between the magnitude of immediate loading and peri-implant bone strength in dogs., Clinical Oral Implants Research, 10.1111/j.1600-0501.2011.02305.x, 23, 11, 1290-1296, 2012.11.
40. Takashi Kono, Yasunori AYUKAWA, Moriyama Yasuko, Kosaku Kurata, Hiroshi Takamatsu, kiyoshi koyano, The Effect of Low-Magnitude, High-Frequency Vibration Stimuli on the Bone Healing of Rat Incisor Extraction Socket., Journal of Biomechanical Engineering, 134, 9, 091001-1-091001-6, 2012.09.
41. Koichi Yamane, Yasunori AYUKAWA, Toru Takeshita, Akihiro Furuhashi, Yoshihisa Yamashita, kiyoshi koyano, Bacterial adhesion affinities of various implant abutment materials, Clinical Oral Implants Research, 10.1111/j.1600-0501.2012.02574.x, in press, 2012.08.
42. Isoda K, Ayukawa Y, Tsukiyama Y, Sogo M, Matsushita Y, Koyano K., Relationship between the bone density estimated by cone-beam computed tomography and the primary stability of dental implants, Clinical Oral Implants Research, 10.1111/j.1600-0501.2011.02203.x, 23, 7, 832-836, 2012.07.
43. Okawachi H, Ayukawa Y, Atsuta I, Furuhashi A, Sakaguchi M, Yamane K, Koyano K., Effect of titanium surface calcium and magnesium on adhesive activity of epithelial-like cells and fibroblasts, Biointerphases, 10.1007/s13758-012-0027-9, 7, 27, 2012.04.
44. Atsuta I, Ayukawa Y,Ogino Y, Moriyama Y, Koyano K., Evaluations of epithelial sealing and peri-implant epithelial down-growth around “step-type” implants, Clinical Oral Implants Research, 10.1111/j.1600-0501.2011.02163.x, 23, 4, 459-466, 2012.04.
45. Akihiro Furuhashi, Yasunori AYUKAWA, ATSUTA IKIRU, Hideyuki Okawachi, kiyoshi koyano, The difference of fibroblast behavior on titanium substrata with different surface characteristics, Odontology, 100, 2, 199-205, 2012.04.
46. Zhang L, Ayukawa Y, LeGeros RZ, Matsuya S, Koyano, K, Ishikawa K., Tissue-response to calcium-bonded titanium surface., Journal of Biomedical Materials Research , 95A, 1, 33-39, 2010.05.
47. Moriyama Y, Ayukawa Y, Ogino Y, Atsuta I, Todo M, Takao Y, Koyano K., Local application of fluvastatin improves peri-implant bone quantity and mechanical property. A rodent study., Acta Biomaterialia, 6, 4, 1610-1618, 2010.04.
48. Masuzaki T, Ayukawa Y, Moriyama Y, Jinno Y, Atsuta I, Ogino Y, Koyano K., The effect of a single remote injection of statin-impregnated poly (lactic-co-glycolic acid) microspheres on osteogenesis around titanium implants in rat tibia., Biomaterials, 31, 12, 3327-3334, 2010.04.
49. Ayukawa Y, Ogino Y, Moriyama Y, Atsuta I, Jinno Y, Kihara M, Tsukiyama Y, Koyano K., Simvastatin enhances bone formation around titanium implants in rat tibiae., Journal of Oral Rehabilitation, 37, 2, 123-130, 2009.11.
50. Moriyama Y, Ayukawa Y, Kabemura K, Matsushita Y, Kihara M, Tsukiyama Y, Todo M, Takao Y, Koyano K., The alteration of peri-implant bone response exposed to static lateral load, Journal of Biomechanical Science and Engineering, 4, 3, 326-335, 2009.07.
51. Jinno Y, Ayukawa Y, Ogino Y, Atsuta I, Tsukiyama Y, Koyano K., Vertical bone augmentation with fluvastatin in an injectable delivery system: a rat study. , Clinical Oral Implants Research, 20, 8, 756-760, 2009.05.
52. Ogino Y, Ayukawa Y, Kukita T, Atsuta I, Koyano K., Platelet-rich plasma suppresses osteoclastogenesis by promoting the secretion of osteoprotegerin in vitro., Journal of Periodontal Research, 44, 2, 217-224, 2009.04.
53. Ayukawa Y, Yasukawa E, Moriyama Y, Ogino Y, Wada H, Atsuta I, Koyano K., Local application of statin promoted bone repair through the suppression of osteoclasts and the enhancement of osteoblasts at bone-healing sites in rats. , Oral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontology, 107, 3, 336-342, 2009.03.
54. Moriyama Y, Ayukawa Y, Ogino Y, Atsuta I, Koyano K., Topical application of statin affects the bone healing around implants, Clinical Oral Implants Research, 19, 6, 600-605, 2008.05.
55. Ogino Y, Ayukawa, Y, Kukita T, Koyano K., The contribution of platelet-derived growth factor, transforming growth factor-beta1 and insulin-like growth factor-I in platelet-rich plasma (PRP) to the proliferation of osteoblast-like cells., Oral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontology, 101:724-729, 2006.06.
56. Ogino Y, Ayukawa, Y, Tsukiyama Y, Koyano K., The effect of platelet-rich plasma on the cellular response of rat bone marrow cells in vitro., Oral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontology, 10.1016/j.tripleo.2005.03.007, 100, 3, 302-307, 100:302-307, 2005.09.
57. Okamura A, Ayukawa Y, Iyama S, Koyano K, Effect of the difference of bone turnover on peri-titanium implant osteogenesis in ovariectomized rats, Journal of Biomedical Materials Research, 10.1002/jbm.a.30110, 70A, 3, 497-505, 70A: 497-505, 2004.07.
58. Ayukawa Y, Okamura A, Koyano K, Simvastatin promotes osteogenesis around titanium implants. A histological and histometrical study in rats, Clinical Oral Implants Research, 10.1111/j.1600-0501.2004.01015.x, 15, 3, 346-350, 15(3):346-350, 2004.06.
59. H. Ikeda, T. Yamaza, M. Yoshinari, Y. Ohsaki, Y. Ayukawa, M.A. Kido, T. Inoue, M. Shimono, K. Koyano, T. Tanaka, Ultrastructural and immunoelectron microscopic studies of the peri-implant epitherium-implant (Ti-6Al-4V) interface of rat maxilla, J Periodontol, 10.1902/jop.2000.71.6.961, 71, 6, 961-973, Vol.71,pp.961-973, 2000.01.
60. Y. Ayukawa, F. Takeshita, T. Inoue, M. Yoshinari, M. Shimono, T. Suetsugu, T. Tanaka, An immunmoelectron microscopic localization of noncollagenous bone proteins (osteocalcin and osteopontin) at the bone-titanium interface, J Biomed Mater Res, 10.1002/(SICI)1097-4636(199807)41:1<111::AID-JBM14>3.0.CO;2-Q, 41, 1, 111-119, Vol.41,pp.111-119, 1998.01.
61. F. Takeshita, K. Murai, S. Iyama, Y. Ayukawa, T. Suetsugu, Uncontrolled diabetes hinders bone formation around titanium implants in rat tibiae, A study using light, fluorescence microscopies and image processing, J Periodontol, 69, 3, 314-320, Vol.69,pp.314-320, 1998.01.
62. Y. Ayukawa, F. Takeshita, M. Yoshinari, T. Inoue, Y. Ohtsuka, M. Shimono, T. Suetsugu, T. Tanaka, An immunocytochemical study for lysosomal cathepsins B and D related to the intracellular degradation of titanium at the bone-titanium interface, J Periodontol, 69, 1, 62-68, Vol.69,pp.62-68, 1998.01.
63. F. Takeshita, Y. Ayukawa, K. Murai, T. Suetsugu, Long-term evaluation of bone-titanium interface in rat tibiae using light microscopy, transmission electron microscopy and image processing, J Biomed Mater Res, 10.1002/(SICI)1097-4636(199711)37:2<235::AID-JBM13>3.0.CO;2-D, 37, 2, 235-242, Vol.37,pp.235-242, 1997.01.
64. F. Takeshita, K. Murai, Y. Ayukawa, T. Suetsugu, Effects of aging on titanium implants inserted into the tibiae of rats using light microscopy, SEM, and image processing,, J Biomed Mater Res, 34, 1, 1-8, Vol.34,pp.1-8, 1997.01.
65. S. Iyama, F. Takeshita, Y. Ayukawa, M. A. Kido, T. Suetsugu, T. Tanaka, A study of regional distribution of bone formed around hydroxyapatite implants in the tibiae of streptozotocin-induced diabetic rats using multiple fluorescent labeling and confocal laser scanning microscopy, J Periodontol, Vol.68,pp.1169-1175, 1997.01.
66. F. Takeshita, Y. Ayukawa, S. Iyama, T. Suetsugu, M. Oishi, Histological comparison of early wound healing following dense HA granule grafting and barrier placement in surgically-created bone defects neighboring implants, J Periodontol, 68, 10, 924-932, Vol.68,pp.924-932, 1997.01.
67. F. Takeshita, H. Takata, Y. Ayukawa, T. Suetsugu, Histomorphometric analysis of the response of rat tibiae to shape memory alloy (nitinol), Biomaterials, 10.1016/S0142-9612(96)00051-8, 18, 1, 21-25, Vol.18,pp.21-25, 1997.01.
68. Y. Ayukawa, F. Takeshita, T. Inoue, M. Yoshinari, Y. Ohtsuka, K. Murai, M. Shimono, T. Suetsugu, T. Tanaka, An ultrastructural study of the bone-titanium interface using pure titanium plastic and pure titanium rod implants, Acta Histochem Cytochem, 29, 3, 243-254, Vol.29,pp.243-254, 1996.01.
69. F. Takeshita, Y. Ayukawa, S. Iyama, T. Suetsugu, M. A. Kido, A histologic evaluation of retrieved hydroxyapatite-coated blade form implants using scanning electron, light, and confocal laser scanning microscopies, J Periodontol, 67, 10, 1034-1040, Vol.67,pp.1034-1040, 1996.01.
70. F. Takeshita, Y. Matsushita, Y. Ayukawa, T. Suetsugu, Fractures of hydroxyapatite-coated blade implants connected with natural teeth. A histological study using SEM, light microscopy, and an image processing system, J Periodontol, 67, 2, 86-92, Vol.67,pp.86-92, 1996.01.
71. K. Murai, F. Takeshita, Y. Ayukawa, T. Kiyoshima, T. Suetsugu, T. Tanaka, Light and electron microscopic studies of bone-titanium interface in the tibiae of young and mature rats, J Biomed Mater Res, 10.1002/(SICI)1097-4636(199604)30:4<523::AID-JBM11>3.0.CO;2-I, 30, 4, 523-533, Vol.30,pp.523-533, 1996.01.