Kyushu University [Koji Nakano (Associate Professor) Faculty of Engineering, Department of Applied Chemistry]

Department of Chemical Systems and Engineering
Graduate School of Engineering

Department of Materials Science and Engineering
School of Engineering

092-802-2890

Doctor of Engineering

Bioanalytical Chemistry

Research Interests

Biosnsesing and bioimaging of odor molecules using nanoreporter particle
keyword : Biosensor, Bioimsging, Nanoreporter particle. Odor molecule
2011.10.
DNA Supramolecular Conjugate for Nanobiosensing Application
keyword : Biosensor, Nanobiodevice, DNA, Supramolecular Architecture
2000.10.
Atomic Force Microscopy Imaging of Biomacromolecules for Chemical Sensing Applications based on Single-Molecule Strategy
keyword : AFM, Bioimaging, Analytical Chemistry, Single molecule
2000.10.
Development of DNA biosensor and gene sensor
keyword : DNA, Gene, Biosensor, Scanning Electrochemical Microscopy, High-Throughput Assay
1992.04.
Direct Electrochemistry of Proteins and Enzymes Attachmed on Self-Assembled Monolayer Electrode and Biosensor Application.
keyword : Bioelectrochemistry, Proteins, Enzymes, Biosensor
1995.10.
Supramolecular Organizate for Chemical Analysisi Applications
keyword : Molecular Organizate, Synthetic Bilayer Membrane, Self-Assembled Monolayer, Multi-Phase Polymer Material
1985.04.

Current and Past Project

The 21st Centry COE, "Functional Innovations of Molecular Informatics"
DNA Ligand-A new DNA Probe for Single Copy Dection of Specific Genes
Investigation of DNA-Based Single Molecular Electrical Devices

Academic Activities

Books

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1.	Koji Nakano,Scanning Electrochemical Microscopy Imaging of DNA Arrays for High Throughput Analysis Applications, in Modern Aspects of Electrochemistry. Applications of Electrochemistry and Nanotechnology in Biology and Medicine, Ed. by N. Eliaz.,Springer,105-142,2011.12. .

Reports

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1.	Koji Nakano,Commentary in the general discussion in Faraday Discussion Vol. 149 Analysis for Healthcare Diagnostics and Theranostics,2011.01. .
2.	Biosensors for Application to Medical Diagnostics. .
3.	RuiQi Zhang, Koji Hirakawa, Masaaki Katayama, Hizuru Nakajima, Nobuaki Soh, Koji Nakano, Toshihiko Imato,Flow Immunoassay Based on Sequential Injection Using Microbeads,Vol. 23, No. 2. pp. 117 - 122,J. Flow Injection Analysis,2006.12. .
4.	DNA Conjugate for Single-Molecular Electronic, Bioelectrochemical Devices. .
5.	DNA Immobilization and Its Application to Gene Sensors. .
6.	Can A DNA Double-Helix Be Applicable To Electroconductive Material?. .

Papers

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1.	Koji Nakano, Hirokazu Yamanouchi, Hisao Yoshinaga, Nobuaki Soh, Toshihiko Imato,Label-free DNA Detection Platform Based on Atomic Force Microscopy Visualisation: Characterising the Molecular-Recognition-Triggered Conformational Changes of an Immobilised Receptor Oligonucleotide Probe,Chem. Commun.,Vol.46,No.13,pp.5683-5685,2010.06. .
2.	Koji Nakano, Yosuke Katsumi, Nobuaki Soh, Toshihiko Imato,An Atomic Force Microscopy Assay of Intercalation Binding, Unwinding and Elongation of DNA, Using a Water-Soluble Psoralen Derivative as a Covalent Binding Probe Molecule,Bull. Chem. Soc. Jpn,Vol.2010,No.3,pp.273-275,2010.03. .
3.	Koji NAKANO, Hideshi MATSUNAGA, Masaharu MURATA, Nobuaki SOH, Toshihiko IMATO,Synthesis Of Circular Double-Stranded DNA Having Single-Stranded Recognition Sequence As Molecular-Physical Probe For Nucleic Acid Hybridization Detection Based On Atomic Force Microscopy Imaging,Anal. Sci.,2009.08. .
4.	K. Nakano, K. Nakamura, K. Iwamoto, N. Soh, T. Imato,Positive-feedback-mode scanning electrochemical microscopy imaging of redox-active DNA-poly(1,4-benzoquinone) conjugate deposited on carbon electrode for micrometer-sized hybridization biosensor applications,J. Electroanal. Chem.,Vol. 628, No. 1, pp. 113–118 ,2009.02. .
5.	K. Nakano, K. Ohkubo, H. Taira, M. Takagi, N. Soh, T. Imato,Synthesis of 1,4-hydroquinone-terminated alkanethiol and self-assembly on gold as characterized by interfacial electrochemistry, electrocatalysis application and ab initio calculation based on comparison with catechol-presenting analogue.,J. Electroanal. Chem.,Vol. 623, No. 1, pp. 49-53,2008.11. .
6.	K. Nakano, K. Ohkubo, H. Taira, M. Takagi, T. Imato,Electrocatalytic oxidation of dihydronicotineamide adenine dinucleotide on gold electrode modified with catechol-terminated alkanethiol self-assembly,Anal. Chim. Acta,Vol. 619, No. 1, pp. 30–36,2008.06. .
7.	N. Soh, K. Makihara, T. Ariyoshi, D. Seto, T. Maki, H. Nakajima, K. Nakano, T. Imato,Phospholipid-linked Coumarin: A Fluorescent Probe for Sensing Hydroxyl Radicals in Lipid Membrane,Analytica Sciences,Vol. 24, No. 2, pp. 293–296,2008.02. .
8.	N. Soh, K. Yoshida, H. Nakajima, K. Nakano, T. Imato, T. Fukaminato, M. Irie,A fluorescent photochromic compound for labeling biomolecules,Chemical Communications,Vol. 2007, No. 28, pp. 5206–5208,2007.12. .
9.	N. Soh, T. Ariyoshi, T. Fukaminato, H. Nakajima, K. Nakano, T. Imato,Swallow-tailed perylene derivative: a new tool for fluorescent imaging of lipid hydroperoxides,Organic Bimolecular Chemistry,Vol. 5, No. 23, pp. 3762–3768,2007.12. .
10.	RuiQi Zhang, Hizuru Nakajima, Nobuaki Soh, Koji Nakano, Takashi Masadome, Kazumi Nagata, Kazuhira Sakamoto, Toshihiko Imato,Sequential Injection Chemiluminescence Immunoassay for Nonionic Surfactants Using Magnetic Microbeads,Analytica Chimica Acta,Vol. 600, No. 1, pp. 105–113,2007.09. .
11.	Koji Nakano, Tadateru Yoshitake, Yasunori Yamashita, Edmond F. Bowden,Cytochrome c Self-Assembly on Alkanethiol Monolayer Electrodes as Characterized by AFM, IR, QCM, and Direct Electrochemistry,Langmuir,Vol.23,No.11,pp.6270-6275,2007.05. .
12.	Yan LI, Jujie REN, Hizuru NAKAJIMA, Nobuaki SOH, Koji NAKANO, Toshihiko IMATO,Surface Plasmon Resonance Immunosensor for IgE Analysis Using Two Types of Anti-IgE Antibodies with Different Active Recognition Sites,Analytical Sciences,Vol. 23, No. 1, pp. 31 ? 38.,2007.01. .
13.	Koji Nakano,Electrical Transport Through DNA-Ferrocene Conjugate as Characterized by Conductive AFM Measurement,Proceedings of The 2nd International Symposium on Functional Innovation of Molecular Informatics,pp. 27,2006.11. .
14.	K. Nakano, G. Hirayama, M. Toguchi, K. Nakamura, K. Iwamoto, N. Soh, T. Imato,Poly(hydroquinone)-coated electrode for immobilizing of 5’-amine functioned capture probe DNA and electrochemical response to DNA hybridization,Science and Technology of Advanced Materials,Vol. 7, No. 7, pp. 718 - 725.,2006.10. .
15.	K. Nakano, H. Matsunaga, K. Sai, N. Soh, T. Imato,Photoactive, covalent attachment of DNA on gold with double-strand specificity using self-assembled monolayers containing psoralen,Anal. Chim. Acta,Vol. 578, No. 1, pp.93 - 99,2006.09. .
16.	Y. LI, M. KOBAYASHI, K. FURUI, N. SOH, K. NAKANO, T. IMATO,Surface plasmon resonance immunosensor for histamine based on an indirect competitive immunoreaction,Analytica Chimica Acta,Vol. 576, No. 1, pp. 77 - 83,2006.08. .
17.	Nobuaki Soh, Daisuke Seto, Koji Nakano, Toshihiko Imato,Novel fuorescent probe for detecting hydroperoxides with strong emission in the visible range,Molecular Biosystems,Vol. 2, No. 2, pp. 81 - 84,2006.06. .
18.	K. Hirakawa, M. Katayama, N. Soh, K. Nakano, H. Ohura, S. Yamasaki, T. Imato,Electrochemical Sandwich Immunoassay for Vitellogenin by Sequential Injection Analysis Using Antibody Immobilized Magnetic Microbeads,Electroanalysis,Vol. 18, No. 13-14, pp. 1297 - 1305,2006.06. .
19.	N. SOH, D. SETO, K. NAKANO, T. IMATO,Methodology of reversible protein labeling for ratiometric fluorescent measurement,Molecular BioSystems,Vol. 2, No. 2, pp. 128 - 131,2006.02. .
20.	K. HIRAKAWA, M. KATAYAMA, N. SOH, K. NAKANO, T. IMATO,Electrochemical Immunoassay for Vitellogenin Based on Sequential Injection Using Antigen-immobilized Magnetic Microbeads,Analytical Sciences,Vol 22, No. 2, pp.81 - 86,2006.01. .
21.	Koji NAKANO, Hideshi MATSUNAGA, Keisuke SAI,Covalent attachment of DNA with double-strand specificity using self-assembled monolayers from psoralen,Proceedings of The 2005 International Chemical Congress of Pacific Basin Societies,pp. 366,2005.12. .
22.	Hideshi MATSUNAGA, Koji NAKANO, Nobuaki SOH, Toshihiko IMATO,Preparation, Characterization and Atomic Force Microscopic Imaging of New DNA Ligand for Single Molecular Hybridization Assay,Proceedings of The 2005 International Chemical Congress of Pacific Basin Societies,pp. 968,2005.12. .
23.	Koji NAKANO, Go HIRAYAMA, Mikiko TOGUCHI, Kaori NAKAMURA, Kaori IWAMOTO, Nobuaki SOH, Toshihiko IMATO,DNA Conjugate Electrode Having DNA/Oligo(1,4-hydroquinone)/PG Interfacial Structure for Biosensor Applications: Characterization by Electrochemical Measurements, QCM Analysis, and AFM Imaging,Program & Abstracts for the 8th Asian Conference on Analytical Sciences,pp. 138,2005.10. .
24.	R-Q. Zhang, K. Hirakawa, D. Seto, N. Soh, K. Nakano, T. Masadome, K. Nagata, K. Sakamoto, T. Imato,Sequential injection chemiluminescence immunoassay for anionic surfactants using magnetic microbeads immobilized with an antibody.,Talanta,VOL. 68, No. 2, pp. 231 - 238,2005.10. .
25.	T. TSURUTA, Y. KATSUMI, S. SHIRAKAWA, S. TAGUCHI, K. NAKANO,Synthesis of New Psoralen Derivatives for Chemical Modification and Functionalization of DNA Duplexes as Characterized by Gel Electrophoresis, IR, QCM, Electrochemistry, and AFM,Abstract Book of International Symposium on Nano-organization and Function,2004.11. .
26.	H. MATSUNGAE, K. SAI, K. NAKANO,Covalent Attachment of Double-Stranded DNA using Self-Assembled Monolayers Containing Psoralen as Characterized by XPS, IR, QCM, Electrochemistry, and AFM,Abstract Book of International Symposium on Nano-organization and Function,pp. 48,2004.11. .
27.	K. NAKANO, K. DOI, K. TAMURA, Y. KATSUMI,Monolayer Formation of Glucose Oxidase Covalently Attached on ω-Aminoalkanethiol/Au via Activation of Carbohydrate Residue of GOx as Characterized by AFM, IR, QCM, and Biosensor Applications,Abstract of The 7th Asian Conference on Analytical Sciences,pp. 308,2004.10. .
28.	T. Anada, H. Matsunaga, R. Karinaga, K. Koumoto, M. Mizu, K. Nakano, S. Shinkai, and K. Sakuraia,Proposal of new modification technique for linear double-stranded DNAs using the polysaccharide schizopyllan,Bioorganic & Medicinal Chemistry Letters,Vol.14, pp.5655 - 5659,2004.09. .
29.	K. Nakano, S. Shirakawa, S. Taguchi,A psoralen Derivative Containing Ferrocene for Redox-labeling of DNA and Electrochemical Gene Sensor Applications,Chemcial Sensors,Vol. 20, Supplement B, pp. 778 - 779.,2004.07. .
30.	Koji Nakano,DNA-Ferrocene Conjugate for Nanoelectronic Material Applications. Atomic Force Microscope-Based, Direct Measurement of Electrical Transport through DNA Molecules,Second 21st Century COE “Towards Creating New Industries Based on Inter-Nanoscience” and 7th SANKEN International Symposium on “Hybridization of Chemistry, Biology, and Material Science”,pp. 38 - 39,2004.01. .
31.	K. Nakano, K. Doi, K. Tamura, Y. Katsumi,Self-Assembling Monolayer Formation of Glucose Oxidase and Cytochrome c Covalently Attached on Alkanethiol Monolayers on Gold,Biomolecular Chemistry. Proceedings of the ISBC 2003,pp. 284 - 285,2003.12. .
32.	K. NAKANO, K. DOI, K. TAMURA, Y. KATSUMI,Self-assembling Monolayer Formation of Glucose Oxidase Covalently Attached on 11-Aminoundecanethiol Monolayers on Gold,Chemical Communications,Vol. 2003, No. 13, pp. 1544 - 1545,2003.06. .

Works, Software and Database

1.	Book cover of Bunseki for 2005 volume..

Presentations

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1.	AFM Imaging of Molecular Beacon DNA Sequence and Single-Molecular Detection of DNA Hybridization.
2.	AFM Imaging of Molecular Beacon DNA Sequence and Single-Molecular Detection of DNA Hybridization.
3.	AFM Imaging of DNA for Application to Single-Molecular Biosaasy.
4.	Development of Molecular Probe for NO Radical Monitoring in Biosystem.
5.	Electrochemical DNA Sensor and its 2D Imaging by Scanning Electrochemical Microscope.
6.	DNA Immobilization on Self-Assembling Monolayer Membrane Prepared by Chemical Vapor Adsorption Method: Characterization by XPS and Application to Hybridization Assay using AFM.
7.	DNA Ligand - A New Strategy of DNA Probe for Single-Molecular Gene Assay.
8.	Single-Molecular Imaging of DNA Conjugate for Nano-, Bio-Science Applications.
9.	Single-Molecular Imaging and Direct Electrochemistry of Cytochrome c Immobilized on Self-Assembled Monolayer Electrode.
10.	Single-Molecular Imaging for Proteins and Enzyme Molecules that Covalently Immobilized on Alkanthiols Monolayer Electrodes.
11.	Single-Molecular Imaging for Proteins and Enzyme Molecules that Covalently Immobilized on Alkanthiols Monolayer Electrodes and Application to Biosensors.
12.	Characterization for Self-Assembly of Glucose Oxidase Molecules upon Covalent Immobilization on Alkanethiol Monolayer Electrode usign Partial Oxidation of Suger Residue and Shiff-Base Formation..

Membership in Academic Society

The Chemical Society of Japan
The Japan Society for Analytical Chemistry
The Electrochemical Society of Japan
The Society of Polymer Science, Japan
The Surface Science Society of Japan
Americal Chemica Society
Royal Society of Chemistry

Awards

Study of Methods for Electrochemcial Analysis based on Molecular Organizates as Chemical Transducer Material
Study of Methods for Chemcial Analysis based on Molecular Organizates as Transducer Material

http://www.imatolab.cstm.kyushu-u.ac.jp/index-j.html.