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Masahiro Goto Last modified date:2018.06.11



Graduate School
Undergraduate School
Other Organization
Administration Post
Other


Homepage
http://www.bioeng.cstm.kyushu-u.ac.jp/ksu_re_center/index.html
TDDS Center .
http://www.bioeng.cstm.kyushu-u.ac.jp/
Goto Lab. .
Academic Degree
Doctor of Engineering
Field of Specialization
Biochemical Engineering, Interfacial Chemistry, Chemical Engineering, Drug Delivery Systems, Cosmetics
Outline Activities
Research
1. Drug delivery systems
2. Cosmetic engineering
3. Immunization by DDS
4. Separation technology (Solvent Extraction &Ion exchange)
5. Bionanotechnology
6. Surfactant chemistry

Education
Biochemical Engineering
Research
Research Interests
  • Novel transdermal systems using bionanocoating techniques
    keyword : DDS, Transdermal delivery, Protein delivery, S/O
    2004.12~2028.03Drug delivery systems using novel S/O techniques.
  • Development of functional cosmetics
    keyword : cosmetics
    2009.01~2028.03.
  • Efficient recovery of precious metal ions using solvent extraction and ion exchange methods
    keyword : Metal recycling, rare metal, solvent extraction, ion exchange
    2007.04~2028.03.
  • Drug Delivery Systems using molecular assembly, emulsion, liposome , micelles.
    keyword : gel, enzyme, DDS, liposome
    1990.06~2028.03Creation of novel function by combining nanotechnology with biotechnology.
  • Biological application of ionic liquids
    keyword : DDS, Transdermal delivery, Protein delivery, microemulsion
    2006.04~2028.03Enzyme reaction in ionic liquids.
Current and Past Project
  • Rare metal recycling project
  • Goto Nano-Bio Project
  • Creation of novel function by combining nanomolecular assembly with biomolecules
Academic Activities
Books
1. Uju, Agung Tri Wijayanta, Masahiro Goto, Noriho Kamiya, High yield hydrolysis of seaweed-waste biomass using peracetic acid and ionic liquid treatments, American Institute of Physics Inc., https://doi.org/10.1063/1.5024058, 1931, 2018.02, Seaweed is one of the most promising bioethanol feedstocks. This water plant has high carbohydrate content but low lignin content, as a result it will be easier to be hydrolysed. This paper described hydrolysis of seaweed-waste biomass from the carrageenan (SWBC) industry using enzymatic saccharification or ionic liquids-HCl hydrolysis. In the first work, SWBC pretreated by peracetic acid (PAA) followed by ionic liquid (IL) caused enhance the cellulose conversion of enzymatic saccharification. At 48h saccharification, the value conversion almost reached 100%. In addition, the untreated SWBC also produced the cellulose conversion 77%. In the second work, SWBC or Bagasse with or without pretreated by PAA was hydrolyzed using ILs-HCl hydrolysis. The ILs used were 1-buthyl-3-methylpyridium chloride, [Bmpy][Cl] and 1-butyl-3-metyl imidazolium chloride ([Bmim][Cl]). [Bmpy][Cl]-HCl hydrolysis produced higher cellulose conversion than [Bmim][Cl]-HCl hydrolysis. The phenomenon was clearly observed on the Bagasse, which without pretreated by PAA. Furthermore, SWBC hydrolyzed by both ILs in the presence low concentration of HCl produced cellulose conversion 70-98% at 60-90 min of hydrolysis time. High cellulose conversion of SWBC on the both hydrolysis was caused by SWBC had the low lignin (4%). Moreover, IL treatments caused lowering of cellulose hydrogen bonds or even changed the cellulose characteristics from cellulose I to cellulose II which easily to be hydrolyzed. In the case of [Bmpy][Cl], this IL may reduce the degree polymerization of celluloses..
2. Momoko Kitaoka, Masahiro Goto, Related topic
Solid-in-oil technique to increase skin permeation
, Springer Japan, https://doi.org/10.1007/978-4-431-56526-0_18, 225-232, 2017.11, Solid-in-oil (S/O) nanodispersion systems represent a new technology that has been developed in the last decade. Nano-sized, solid-state, hydrophilic drug molecules can be dispersed in an oil vehicle by coating the drug with hydrophobic surfactants; these materials are designated here as S/O nanodispersions. Conventional S/O nanodispersion systems were devised to maintain hydrophilic enzymes in their active form in organic solvents. Because the coated molecules are stable in non-aqueous media, S/O nanodispersion systems can be successfully applied in oral and skin drug deliveries. It is known that the permeability of hydrophilic drugs through the skin decreases when the molecular weight of the drug exceeds 500 Da. In addition, hydrophobic molecules tend to permeate through the skin preferentially, compared with hydrophilic molecules, because the outermost layer of the skin is hydrophobic. In our experiments, the permeation of hydrophilic biomolecules such as peptides and proteins through the skin increased by 4-7 times when their molecules were coated with lipophilic surfactants and dispersed in an oil vehicle. Here, we introduce an efficient method for drug delivery through the skin, using the S/O nanodispersion technique..
3. M. Moniruzzaman, H. Mahmood, Masahiro Goto, Ionic Liquid Based Nanocarriers for Topical and Transdermal Drug Delivery, Royal Society of Chemistry, <core:doi>https://doi.org/10.1039/9781788011839-00390, 390-403, 2018.01, In the pharmaceutical industry, there are challenges in topical and transdermal administration of drugs, which are sparingly soluble in water and most organic solvents. Ionic liquids (ILs) have been found to be very effective for dissolution of sparingly soluble drugs. However, hydrophilic IL-borne drugs cannot penetrate into or across the skin because of the highly hydrophobic barrier function of the outer skin. In this chapter we report a novel IL-in-oil (IL/o) microemulsion (ME) that is able to dissolve a significant amount of sparingly soluble drug, acyclovir, in the IL core while the continuous oil phase can provide the desired features for topical/transdermal transport through the skin. The ME is composed of a blend of the nonionic surfactants polyoxyethylene sorbitan monooleate (Tween 80) and sorbitan laurate (Span 20), isopropyl myristate (IPM) as an oil phase, and the IL [C<sub>1</sub>mim][(MeO)<sub>2</sub>PO<sub>2</sub>] (dimethylimidazolium dimethylphosphate) as a dispersed phase. The size and size distribution of the aggregates in the MEs were characterized by dynamic light scattering, showing formation of the nanocarrier in the size range 8-34 nm. In vitro drug permeation studies into and across the skin showed that the IL/o ME increased drug administration compared with other formulations. The safety profile of the new carrier was evaluated using a cytotoxicity assay on the human epidermal model LabCyte. We believe that these IL-assisted nonaqueous MEs can serve as a versatile and efficient nanodelivery system for sparingly soluble drug molecules..
4. Masahiro Goto, Transcutaneous Immunization Using Nano-sized Drug Carriers, Nanomaterials in Pharmacology, Humana Press, 2016.12.
5. Masahiro Goto, Application of Ionic Liquids on Rare Earth Green Separation and Utilization, 2016.04.
6. Development & Applications of Transdermal Drug Delivery Systems.
7. Rare metal separation by solvent extraction using ionic liquids.
8. Muhammad Moniruzzaman and Masahiro Goto, Nanoscale Biocatalysis, Humana Press, Chapter 4, Molecular Assembly Assisted Biocatalytic Reactions in Ionic Liquids, pp37-50, 2011.05.
9. Masahiro Goto, Ion Exchange and Solvent Extraction, Marcel Dekker, Volume 14, 2001.10.
Reports
1. Masahiro Goto, Solid-in-oil nanodispersions for transdermal drug delivery systems, Biotechnol. J., , 2016.11.
2. Masahiro Goto, Ionic liquids as a potential tool for drug delivery systems, Med.Chem.Comm., 2016.09.
3. Masahiro Goto, Recent advances in exploiting ionic liquids for biomolecules: Solubility, stability, and applications, Biotechnol. J., , 2016.08.
4. Masahiro Goto, Ionic liquids pretreatment combined with enzymatic hydrolysis of lignocellulosic biomass for production of fermentable sugar, Biochemical Eng. J, 2016.07.
5. Masahiro Goto, Solid-in-Oil Dispersion: A Novel Core Technology for Drug Delevery Systems, Int. J. Pharm., 438, 249-257, 2012.11.
6. Masahiro Goto, Application of Ionic Liquids for Separation of Rare Earth Metals, Solv. Extr. Res. Dev. Jpn, 19, 17-28, (2012), 2012.05.
7. M. Moniruzzaman, M. Goto, Application of ionic liquids: Future Solvents and Reagents for Pharmaceuticals, J. Chem. Eng. Japan, Vol.44, No.6, 2011.06.
8. Y. Baba, F.Kubota, N. Kamiya and M. Goto, Recent Advances in Extraction and Separation of Rare Earth Metals Using Ionic Liquids, J. Chem. Eng. Japan, 2011.11.
9. M. Moniruzzaman, N. Kamiya and M. Goto, Activation and stabilization of enzymes in ionic liquids, Org. Biomol. Chem., 8, 2887-2899, 2010.05.
10. M. Moniruzzaman, K. Nakashima, N. Kamiya and M. Goto, Recent advances of enzymatic reactions in ionic liquids, Biochem. Eng. J., 48, 295-314, 2010.04.
11. Ionic Liquids for novel biocatalysis reactions.
Papers
1. M. Takano, Y. Ozaki, S. Asano, Y. Baba, F. Kubota, M. Goto, Separation of Cobalt by Solvent Extraction Using Oxide-Amine Mixture System, J. MMIJ., 136, 8, 188-192, 2017.08.
2. Meysam Lotfi, Muhammad Moniruzzaman, Magaret Sivapragasam, Shalini Kandasamy, M. I. Abdul Mutalib, Noorjahan Banu Alitheen, Masahiro Goto, Solubility of acyclovir in nontoxic and biodegradable ionic liquids
COSMO-RS prediction and experimental verification, Journal of Molecular Liquids, https://doi.org/10.1016/j.molliq.2017.08.020, 243, 124-131, 2017.10, The pharmaceutical industry faces a challenge to find potential solvents for drug molecules that are sparingly soluble in water and conventional organic solvents. Recently, ionic liquids (ILs) have attracted great attention as pharmaceutical solvents owing to their unique physicochemical and biological properties. In this study, the solubility of the sparingly soluble drug molecule acyclovir (ACV) in a wide variety of ILs was investigated by conductor-like screening model for real solvents (COSMO-RS) calculations. The predicted solubilities were validated by experimental measurements, and good agreement was found between the predicted and experimental results. The solubility of ACV was greatly affected by the structure of ILs, particularly the anionic moiety. Among the various ILs tested, ACV showed excellent solubility in ammonium-based ILs with an acetate anion. In vitro cytotoxicity of ILs to the MCF-10 normal breast epithelial cell line and cancer cell lines (MDA MB 231 and MCF 7) was investigated. The ammonium-based ILs showed higher IC50 values than the imidazolium-based ILs with the acetate anion. Biodegradability results showed that diethylammonium acetate, triethylammonium acetate, and choline acetate ILs have high levels of biodegradation under aerobic conditions and can be classified as readily biodegradable. These findings will be useful for the design of IL-based drug delivery carriers that can act as versatile and efficient drug delivery systems for sparingly soluble drug molecules..
3. Wataru Yoshida, Yuzo Baba, Fukiko Kubota, Noriho Kamiya, Masahiro Goto, Extraction and stripping behavior of platinum group metals using an amic-acid-type extractant, Journal of Chemical Engineering of Japan, https://doi.org/10.1252/jcej.16we335, 50, 7, 521-526, 2017.07, The recovery and separation of platinum group metals (PGMs) are of considerable significance for metal sustainability. To establish an efficient extraction process for PGMs, the novel extractant N-[N,N-di(2-ethylhexyl)aminocarbonylmethyl] phenylalanine (D2EHAF), containing a phenylalanine moiety as the metal-affinity group, was synthesized. The extraction of PGMs from aqueous HCl and HNO3 solutions with D2EHAF, or our previously developed N-[N,N-di(2-ethylhexyl) aminocarbonylmethyl]glycine (D2EHAG), in n-dodecane was investigated. Both D2EHAF and D2EHAG exhibited high extraction affinities for Os4+, Pt4+, and Pd2+ in aqueous HCl. In a HNO3 solution, D2EHAF extracted Pd2+ more efficiently than D2EHAG, and the extraction of Pd2+ from a 1 mol dm-3 HNO3 solution with D2EHAF proceeded quantitatively. The extracted metal ions were stripped from the organic solution using a highly acidic solution, or thiourea..
4. Yoshiro Tahara, Masahiro Goto, Transdermal protein delivery and immunization by a solid-in-oil nanodispersion technique, Drug Delivery System, https://doi.org/10.2745/dds.32.176, 32, 3, 176-183, 2017.04, Transcutaneous vaccination is an attractive strategy that delivers antigen molecules topically into the skin to induce protective or therapeutic immune responses. In the last two decades, the skin has been regarded as a potential administration site for vaccines due to the abundant antigen presenting cells in the skin such as Langerhans cells and dermal dendritic cells, which are found in the epidermis or dermis. To create an efficient transcutaneous vaccine, the antigen needs to be penetrated across the stratum corneum, which is the outermost layer of the skin and possesses a high barrier function. To overcome this issue, various types of drug delivery systems have been proposed such as ultrasound, jet immunization and microneedles as physical methods, and penetration peptides, liposomes and nanoparticles as chemical methods. In this review, a solid-in-oil (S/O) nanodispersion, which is an oil-based drug carrier for proteins and peptides, and its application for transcutaneous protein delivery and vaccination are summarized. Along with comparing the S/O nanodispersion with the other oil-based drug carriers, the transdermal delivery of proteins such as insulin is introduced. Finally, basic immunological responses via transcutaneous administration with the S/O nanodispersion containing a model antigen, and researches on protect and therapeutic applications are summarized..
5. Mansoor Ul Hassan Shah, Magaret Sivapragasam, Muhammad Moniruzzaman, Md Mahabubur Rahman Talukder, Suzana Bt Yusup, Masahiro Goto, Production of sophorolipids by Starmerella bombicola yeast using new hydrophobic substrates, Biochemical Engineering Journal, https://doi.org/10.1016/j.bej.2017.08.005, 127, 60-67, 2017.04, Sophorolipids (SLs) are surface active compounds that have excellent surface-lowering properties. Typically, SLs are produced using different hydrophobic substrates, such as alkanes, vegetable oils, and industrial effluents. The properties of the SLs are highly dependent on the hydrophobic substrate used for their production. The aim of the present study is to investigate the properties of SLs produced using three new hydrophobic substrates: Tapis oil, Melita oil, and Ratawi oil. The structures of the SLs were determined by Fourier transform infrared spectroscopy. The SLs yields using Tapis, Melita, and Ratawi oil were 26, 21, and 19 g L−1, respectively. The SLs produced using Tapis, Melita, and Ratawi oil reduced the surface tension of pure water to 36.38, 37.84, and 38.92 mN/m, respectively, corresponding to critical micelle concentrations (CMCs) of 54.39, 55.68, and 58.34 mg L−1. These values are comparable with SLs produced using palm oil (surface tension 35.38 mN/m and CMC 48.76 mg L−1). The SLs produced using Tapis oil show a maximum emulsification activity of 71.2%. The produced SLs are active over the pH range 2–10 and for salinity up to 20% (w/v) NaCl. Thus, they show potential for various applications, including microbial enhanced oil recovery and oil spill remediation..
6. Fukiko Kubota, Eiko Shigyo, Wataru Yoshida, Masahiro Goto, Extraction and separation of Pt and Pd by an imidazolium-based ionic liquid combined with phosphonium chloride, Solvent Extraction Research and Development, https://doi.org/10.15261/serdj.24.97, 24, 2, 97-104, 2017.05, Extraction and separation of Pt(IV) and Pd(II) from a hydrochloric acid solution was examined with a mixture of undiluted ionic liquids, 1-methyl-3-octylimidazolium bis(trifluoromethylsulfonyl)imide ([C8mim][Tf2N]) and trihexyltetradecylphosphonium chloride ([P6,6,6,14][Cl]). Imidazolium-based [C8mim][Tf2N] shows a high selectivity for Pt(VI), whereas [P6,6,6,14][Cl] has a high extraction ability for both Pt(IV) and Pd(II). The addition of [P6,6,6,14][Cl] to [C8mim][Tf2N] improved the extraction efficiency for Pt and the separation factor between Pt and Pd also increased. This improvement was attributed to the presence of Cl- being more hydrophilic than Tf2N- in the [C8mim][Tf2N] extraction phase. Stripping of the metals was possible with HNO3 solution without any degradation of the ionic liquid extraction phase. The ionic liquid mixture was shown to be reusable for at least five extraction cycles..
7. Yuji Matsumoto, Atsuomi Shundo, Masashi Ohno, Nobutomo Tsuruzoe, Masahiro Goto, Keiji Tanaka, Evolution of heterogeneity accompanying sol-gel transitions in a supramolecular hydrogel, Soft Matter, https://doi.org/10.1039/c7sm01612c, 13, 40, 7433-7440, 2017.04, When a peptide amphiphile is dispersed in water, it self-assembles into a fibrous network, leading to a supramolecular hydrogel. When the gel is physically disrupted by shaking, it transforms into a sol state. After aging at room temperature for a while, it spontaneously returns to the gel state, called sol-gel transition. However, repeating the sol-gel transition often causes a change in the rheological properties of the gel. To gain a better understanding of the sol-gel transition and its reversibility, we herein examined the thermal motion of probe particles at different locations in a supramolecular hydrogel. The sol obtained by shaking the gel was heterogeneous in terms of the rheological properties and the extent decreased with increasing aging time. This time course of heterogeneity, or homogeneity, which corresponded to the sol-to-gel transition, was observed for the 1st cycle. However, this was not the case for the 2nd and 3rd cycles; the heterogeneity was preserved even after aging. Fourier-transform infrared spectroscopy, small-angle X-ray scattering, and atomic force and confocal laser scanning microscopies revealed that, although the molecular aggregation states of amphiphiles both in the gel and sol remained unchanged with the cycles, the fibril density diversified to high and low density regions even after aging. The tracking of particles with different sizes indicated that the partial mesh size in the high density region and the characteristic length scale of the density fluctuation were smaller than 50 nm and 6 μm, respectively..
8. Norasikin Othman, Norul Fatiha Mohamed Noah, Lim Yin Shu, Zing Yi Ooi, Norela Jusoh, Mariani Idroas, Masahiro Goto, Easy removing of phenol from wastewater using vegetable oil-based organic solvent in emulsion liquid membrane process, Chinese Journal of Chemical Engineering, https://doi.org/10.1016/j.cjche.2016.06.002, 25, 1, 45-52, 2017.01, Phenol is considered as pollutant due to its toxicity and carcinogenic effect. Thus, variety of innovative methods for separation and recovery of phenolic compounds is developed in order to remove the unwanted phenol from wastewater and obtain valuable phenolic compound. One of potential method is extraction using green based liquid organic solvent. Therefore, the feasibility of using palm oil was investigated. In this research, palm oil based organic phase was used as diluents to treat a simulated wastewater containing 300 × 10-6of phenol solution using emulsion liquid membrane process (ELM). The stability of water-in-oil (W/O) emulsion on diluent composition and the parameters affecting the phenol removal efficiency and stability of the emulsion; such as emulsification speed, emulsification time, agitation speed, surfactant concentration, pH of external phase, contact time, stripping agent concentration and treat ratio were carried out. The results of ELM study showed that at ratio 7 to 3 of palm oil to kerosene, 5 min and 1300 r·min− 1of emulsification process the stabile primary emulsion were formed. Also, no carrier is needed to facilitate the phenol extraction. In experimental conditions of 500 r·min− 1of agitation speed, 3% Span 80, pH 8 of external phase, 5 min of contact time, 0.1 mol·L− 1NaOH as stripping agent and 1:10 of treat ratio, the ELM process was very promising for removing the phenol from the wastewater. The extraction performance at about 83% of phenol was removed for simulated wastewater and an enrichment of phenol in recovery phase as phenolate compound was around 11 times..
9. Rie Wakabayashi, Kensuke Yahiro, Kounosuke Hayashi, Masahiro Goto, Noriho Kamiya, Protein-Grafted Polymers Prepared Through a Site-Specific Conjugation by Microbial Transglutaminase for an Immunosorbent Assay, Biomacromolecules, https://doi.org/10.1021/acs.biomac.6b01538, 18, 2, 422-430, 2017.02, Protein-polymer conjugates have been developed in many fields. Most hybrids are composed of one protein attached to one or several polymer chains. The other form of hybrid involves the construction of multiple proteins on one polymer chain, thereby facilitating protein assemblies that provide multivalent effects. Unfortunately, synthetic methods for production of these types of hybrids are limited and challenging because precise control of the conjugation sites is needed. Herein, a novel synthetic polymer that can enzymatically assemble multiple proteins was developed. Polyacrylamide grafted with multiple microbial transglutaminase (MTG)-recognizable peptide derivatives was synthesized, and MTG-catalyzed site-specific conjugation of proteins with the polymer was achieved. The application for immunological biosensing was demonstrated using the assembly of a fusion protein composed of antibody-binding and enzyme moieties. This enzymatic method to synthesize a one-dimensional protein assembly on a synthetic polymer is versatile and can be expanded to a wide range of applications..
10. Qingliang Kong, Momoko Kitaoka, Rie Wakabayashi, Noriho Kamiya, Masahiro Goto, Transcutaneous immunotherapy of pollinosis using solid-in-oil nanodispersions loaded with T cell epitope peptides, International Journal of Pharmaceutics, https://doi.org/10.1016/j.ijpharm.2017.07.020, 529, 1-2, 401-409, 2017.08, Pollinosis, a typical seasonal allergy, is a serious public health problem. Limited numbers of patients receive curative immunotherapy instead of symptomatic therapy; however, there are still some concerns about the inconvenience and side effects of subcutaneous injections and sublingual administration caused by immunotherapy. Here, we propose a simple and safe transcutaneous immunotherapy using solid-in-oil (S/O) nanodispersions loaded with vaccine T cell epitope peptides derived from pollen allergen. S/O nanodispersions are oil-based dispersions of antigens coated with hydrophobic surfactants. They have a high potential to deliver biomolecules including peptides or proteins to immune cells in the skin, and to induce an immune response. The result of quantitative and qualitative analysis by in vitro permeation experiments demonstrated the effective permeation of T cell epitope peptides into the skin. Furthermore, in vivo experiments using a pollinosis mouse model indicated that the S/O nanodispersions loaded with T cell epitopes suppressed serum antibody IgE and cytokine production, and alleviated allergic symptoms to a similar therapeutic level to that observed for subcutaneous injection. These results indicate the potential of transcutaneous immunotherapy using S/O nanodispersions for the future treatment of pollinosis..
11. Momoko Kitaoka, Masahiro Goto, Related topic
Solid-in-oil technique to increase skin permeation, Skin Permeation and Disposition of Therapeutic and Cosmeceutical Compounds, https://doi.org/10.1007/978-4-431-56526-0_18, 225-232, 2017.11, Solid-in-oil (S/O) nanodispersion systems represent a new technology that has been developed in the last decade. Nano-sized, solid-state, hydrophilic drug molecules can be dispersed in an oil vehicle by coating the drug with hydrophobic surfactants; these materials are designated here as S/O nanodispersions. Conventional S/O nanodispersion systems were devised to maintain hydrophilic enzymes in their active form in organic solvents. Because the coated molecules are stable in non-aqueous media, S/O nanodispersion systems can be successfully applied in oral and skin drug deliveries. It is known that the permeability of hydrophilic drugs through the skin decreases when the molecular weight of the drug exceeds 500 Da. In addition, hydrophobic molecules tend to permeate through the skin preferentially, compared with hydrophilic molecules, because the outermost layer of the skin is hydrophobic. In our experiments, the permeation of hydrophilic biomolecules such as peptides and proteins through the skin increased by 4-7 times when their molecules were coated with lipophilic surfactants and dispersed in an oil vehicle. Here, we introduce an efficient method for drug delivery through the skin, using the S/O nanodispersion technique..
12. Mari Takahara, Rie Wakabayashi, Kosuke Minamihata, Masahiro Goto, Noriho Kamiya, Primary Amine-Clustered DNA Aptamer for DNA-Protein Conjugation Catalyzed by Microbial Transglutaminase, Bioconjugate Chemistry, https://doi.org/10.1021/acs.bioconjchem.7b00594, 28, 12, 2954-2961, 2017.12, DNA-protein conjugates are promising biomolecules for use in areas ranging from therapeutics to analysis because of the dual functionalities of DNA and protein. Conjugation requires site-specific and efficient covalent bond formation without impairing the activity of both biomolecules. Herein, we have focused on the use of a microbial transglutaminase (MTG) that catalyzes the cross-linking reaction between a glutamine residue and a primary amine. In a model bioconjugation, a highly MTG-reactive Gln (Q)-donor peptide (FYPLQMRG, FQ) was fused to enhanced green fluorescent protein (FQ-EGFP) and a primary amine-clustered DNA aptamer was enzymatically synthesized as a novel acyl-acceptor substrate of MTG, whose combination leads to efficient and convenient preparation of DNA-protein conjugates with high purity. Dual functionality of the obtained DNA-EGFP conjugate was evaluated by discrimination of cancer cells via c-Met receptor recognition ability of the DNA aptamer. The DNA aptamer-EGFP conjugate only showed fluorescence toward cells with c-Met overexpression, indicating the retention of the biochemical properties of the DNA and EGFP in the conjugated form..
13. Fukiko Kubota, Riho Kono, Wataru Yoshida, Maha Sharaf, Spas D. Kolev, Masahiro Goto, Recovery of gold ions from discarded mobile phone leachate by solvent extraction and polymer inclusion membrane (PIM) based separation using an amic acid extractant, Separation and Purification Technology, https://doi.org/10.1016/j.seppur.2018.04.031, 2018.01, This paper reports on the selective separation and recovery of gold ions from leachates of discarded mobile phones using liquid-liquid extraction and a polymer inclusion membrane (PIM) transport system. The collected mobile phones were crushed by a mill and the obtained powder was calcinated. After leaching with aqua regia, the metal composition of the leachate was analyzed by inductively coupled plasma atomic emission spectrometry (ICP-AES). The analysis results confirmed that the mobile phone waste contained 397 g/ton of gold. Liquid-liquid extraction and PIM-based separation procedures for the selective recovery of gold(III) from synthetic and actual leachates were developed. The extracting organic solution and the PIM incorporated the newly synthesized extractant N-[N,N-di(2-ethylhexyl)aminocarbonylmethyl]glycine (D2EHAG) which exhibited high selectivity for the gold(III) ion over the other metal ions present in much higher concentrations in the leachates. The compositions of the feed and receiving solutions in both the liquid-liquid and PIM based extraction and back-extraction of the gold(III) ions were optimized. It was established that optimal extraction required a HCl concentration in the feed solution of 2 mol/L and that a receiving solution containing 0.1 M thiourea in 1 M HCl was capable of back-extracting gold(III) quantitatively. Membrane transport experiments with a synthetic leachate as the feed solution demonstrated that 96% of the gold(III) ions was selectively transported into the receiving solution of the transport cell thus leaving all other metal ions in the leachate..
14. M. Moniruzzaman, H. Mahmood, Masahiro Goto, Ionic Liquid Based Nanocarriers for Topical and Transdermal Drug Delivery, Ionic Liquid Devices, https://doi.org/10.1039/9781788011839-00390, 390-403, 2018.01, In the pharmaceutical industry, there are challenges in topical and transdermal administration of drugs, which are sparingly soluble in water and most organic solvents. Ionic liquids (ILs) have been found to be very effective for dissolution of sparingly soluble drugs. However, hydrophilic IL-borne drugs cannot penetrate into or across the skin because of the highly hydrophobic barrier function of the outer skin. In this chapter we report a novel IL-in-oil (IL/o) microemulsion (ME) that is able to dissolve a significant amount of sparingly soluble drug, acyclovir, in the IL core while the continuous oil phase can provide the desired features for topical/transdermal transport through the skin. The ME is composed of a blend of the nonionic surfactants polyoxyethylene sorbitan monooleate (Tween 80) and sorbitan laurate (Span 20), isopropyl myristate (IPM) as an oil phase, and the IL [C1mim][(MeO)2PO2] (dimethylimidazolium dimethylphosphate) as a dispersed phase. The size and size distribution of the aggregates in the MEs were characterized by dynamic light scattering, showing formation of the nanocarrier in the size range 8-34 nm. In vitro drug permeation studies into and across the skin showed that the IL/o ME increased drug administration compared with other formulations. The safety profile of the new carrier was evaluated using a cytotoxicity assay on the human epidermal model LabCyte. We believe that these IL-assisted nonaqueous MEs can serve as a versatile and efficient nanodelivery system for sparingly soluble drug molecules..
15. Maha Sharaf, Wataru Yoshida, Fukiko Kubota, Spas D. Kolev, Masahiro Goto, A polymer inclusion membrane composed of the binary carrier PC-88A and Versatic 10 for the selective separation and recovery of Sc, RSC Advances, https://doi.org/10.1039/c7ra12697b, 8, 16, 8631-8637, 2018.01, This study reports on the selective separation of scandium (Sc) from other rare earth metals (REMs) using a polymer inclusion membrane (PIM). The PIM prepared with PC-88A (2-ethylhexyl hydrogen-2-ethylhexylphosphonate) alone as the carrier showed high extractability but the poor back-extraction of the extracted Sc3+ ions did not allow the transport of these ions to the receiving solution of a membrane transport system. To overcome this problem, a novel approach was introduced using a mixture of carriers that allowed Sc3+ transport into the receiving solution. A cellulose triacetate (CTA) based PIM containing both PC-88A and Versatic 10 (decanoic acid) as carriers and dioctyl phthalate (DOP) as a plasticizer was prepared for the selective separation of Sc3+ from other REM ions in nitrate media. The membrane composition was optimized and the effect of operational parameters such as pH of the feed solution and composition of the receiving solution was explored. The flux at the membrane/feed solution interface was found to depend significantly on the carrier concentration in the PIM, pH of the feed solution and the receiving solution acidity. The newly developed PIM allowed quantitative and selective transport of Sc3+ thus demonstrating its suitability for the selective recovery of this metal..
16. Rie Wakabayashi, Masato Sakuragi, Shuto Kozaka, Yoshiro Tahara, Noriho Kamiya, Masahiro Goto, Solid-in-Oil Peptide Nanocarriers for Transcutaneous Cancer Vaccine Delivery against Melanoma, Molecular Pharmaceutics, https://doi.org/10.1021/acs.molpharmaceut.7b00894, 15, 3, 955-961, 2018.03, Cancer vaccines represent a prophylactic or therapeutic method of suppressing cancer by activating the adaptive immune system. The immune response is initiated by the delivery of tumor antigens to antigen presenting cells (APCs). The use of peptides as vaccine antigens is advantageous, especially in the availability and productivity of pure and defined antigens. However, their limited immunogenicity remains a major drawback, and therefore, the utilization of nanocarriers as a means of delivering antigens to target cells and/or the addition of immune stimulants have been investigated as an efficient peptide-based cancer vaccine. We have developed a solid-in-oil (S/O) nanodispersion as a transcutaneous nanocarrier for hydrophilic molecules. This system has attractive features as a peptide nanocarrier for cancer vaccines, including transcutaneous targeting of professional APCs in the skin, high encapsulation efficacy of hydrophilic molecules, and capacity for coloading with a variety of immune stimulants such as adjuvants. We therefore sought to utilize the developed S/O nanodispersion for the delivery of the tyrosine-related protein 2 peptide, TRP-2180-188, as a peptide antigen against melanoma. Transcutaneous vaccination of the S/O nanodispersion coloaded with adjuvant R-848 was associated with a significant inhibition of melanoma growth and suppression of lung metastasis in tumor-bearing mice. Our findings indicate the potential of S/O nanodispersions as an endogenous peptide carrier for cancer vaccines..
17. Muhamad Alif Razi, Rie Wakabayashi, Yoshiro Tahara, Masahiro Goto, Noriho Kamiya, Genipin-stabilized caseinatehitosan nanoparticles for enhanced stability and anti-cancer activity of curcumin, Colloids and Surfaces B: Biointerfaces, https://doi.org/10.1016/j.colsurfb.2018.01.041, 164, 308-315, 2018.03, Nanoparticles formed by the assembly of protein and polysaccharides are of great interest for the delivery of hydrophobic molecules. Herein, the formation of genipin-crosslinked nanoparticles from caseinate (CS) and chitosan (CH) is reported for the delivery of curcumin, a polyphenolic compound from turmeric, to cells. Genipin-crosslinked CS-CH nanoparticles (G-CCNPs) having a diameter of ∼250 nm and a low polydispersity index showed excellent stability over a wide pH range, as indicated by dynamic light scattering and transmission electron microscopic measurements. Cellular uptake of curcumin loaded into G-CCNPs by HeLa cells was improved, as measured by confocal laser scanning microscopy (CLSM) and fluorescence-activated cell-sorting analysis. Cell proliferation assays indicated that G-CCNPs were nontoxic and that curcumin's anticancer activity in vitro was also improved by G-CCNPs. Stability of curcumin at neutral pH was enhanced by G-CCNPs. CLSM study revealed that G-CCNPs were poorly internalized by HeLa cells, possibly because of strong cell membrane interactions and a negative zeta potential. Overall, our results suggested that the enhanced curcumin cytotoxicity might be associated with the enhanced stability of curcumin by G-CCNPs and free curcumin released from G-CCNPs into the cell. These biocompatible NPs might be suitable carriers for enhancing curcumin's therapeutic potential..
18. Mochamad L. Firmansyah, Fukiko Kubota, Masahiro Goto, Solvent extraction of Pt(IV), Pd(II), and Rh(III) with the ionic liquid trioctyl(dodecyl) phosphonium chloride, Journal of Chemical Technology and Biotechnology, https://doi.org/10.1002/jctb.5544, 93, 6, 1714-1721, 2018.04, BACKGROUND: Recycling of spent platinum group metals (PGMs) has attracted much attention in order to overcome the problems associated with the low natural abundance of these resources. To recycle such metals, efficient hydrometallurgical processes are required. To improve the efficiency of these processes, we have designed a new phosphonium-based ionic liquid as an extraction solvent. This paper reports on the potential use of the ionic liquid for extraction and separation of PGMs, namely Pt(IV), Pd(II), and Rh(III). RESULTS: An ionic liquid, trioctyl(dodecyl)phosphonium chloride, P88812Cl, which was newly synthesized, showed highly efficient extraction for Pt(IV), Pd(II), and Rh(III). P88812Cl features several advantages as an extraction solvent, such as high hydrophobicity and low viscosity compared with those features of a commercial analogue, trihexyl(tetradecyl)phosphonium chloride (P66614Cl). The favorable features of our novel ionic liquid were reflected by its high extraction efficiency and low release of phosphorus from the ionic liquid into the aqueous feed solution. Stripping operations were possible, and the high reusability of the ionic liquid was confirmed. CONCLUSION: The novel ionic liquid P88812Cl is potentially useful as an extraction solvent for PGMs. Results highlight the strengths of ionic liquids and the tuneability of their properties through design of their molecular structure..
19. Safrina Dyah Hardiningtyas, Rie Wakabayashi, Momoko Kitaoka, Yoshiro Tahara, Kosuke Minamihata, Masahiro Goto, Noriho Kamiya, Mechanistic investigation of transcutaneous protein delivery using solid-in-oil nanodispersion
A case study with phycocyanin, European Journal of Pharmaceutics and Biopharmaceutics, https://doi.org/10.1016/j.ejpb.2018.01.020, 127, 44-50, 2018.03, Phycocyanin (PC), a water-soluble protein-chromophore complex composed of hexameric (αβ)6 subunits, has important biological functions in blue-green algae as well as pharmacological activities in biomedicine. We have previously developed a solid-in-oil (S/O) nanodispersion method to deliver biomacromolecules through the skin, although the transcutaneous mechanism has not yet been fully elucidated. To study the mechanism of transcutaneous protein delivery, we therefore enabled S/O nanodispersion by coating PC with hydrophobic surfactants and evaluated how the proteinaceous macromolecules formulated in an oil phase might permeate the skin. The extent of S/O nanodispersion of PC was dependent on the type of surfactant, suggesting that the selection of a suitable surfactant is crucial for encapsulating a large protein having a subunit structure. By measuring the intrinsic fluorescence of PC, we found that S/O nanodispersion facilitated the accumulation of PC in the stratum corneum (SC) of Yucatan micropig skin. Furthermore, after crossing the SC layer, the fluorescent recovery of PC was evident, indicating the release of the biologically active form of PC from the SC into the deeper skin layer..
20. Rahman Md Moshikur, Md Raihan Chowdhury, Rie Wakabayashi, Yoshiro Tahara, Muhammad Moniruzzaman, Masahiro Goto, Characterization and cytotoxicity evaluation of biocompatible amino acid esters used to convert salicylic acid into ionic liquids, International Journal of Pharmaceutics, https://doi.org/10.1016/j.ijpharm.2018.05.021, 546, 1-2, 31-38, 2018.03, The technological utility of active pharmaceutical ingredients (APIs) is greatly enhanced when they are transformed into ionic liquids (ILs). API-ILs have better solubility, thermal stability, and the efficacy in topical delivery than solid or crystalline drugs. However, toxicological issue of API-ILs is the main challenge for their application in drug delivery. To address this issue, 11 amino acid esters (AAEs) were synthesized and investigated as biocompatible counter cations for the poorly water-soluble drug salicylic acid (Sal) to form Sal-ILs. The AAEs were characterized using 1H and 13C NMR, FTIR, elemental, and thermogravimetric analyses. The cytotoxicities of the AAE cations, Sal-ILs, and free Sal were investigated using mammalian cell lines (L929 and HeLa). The toxicities of the AAE cations greatly increased with inclusion of long alkyl chains, sulfur, and aromatic rings in the side groups of the cations. Ethyl esters of alanine, aspartic acid, and proline were selected as a low cytotoxic AAE. The cytotoxicities of the Sal-ILs drastically increased compared with the AAEs on incorporation of Sal into the cations, and were comparable to that of free Sal. Interestingly, the water miscibilities of the Sal-ILs were higher than that of free Sal, and the Sal-ILs were miscible with water at any ratio. A skin permeation study showed that the Sal-ILs penetrated through skin faster than the Sal sodium salt. These results suggest that AAEs could be used in biomedical applications to eliminate the use of traditional toxic solvents for transdermal delivery of poorly water-soluble drugs..
21. Momoko Kitaoka, Masahiro Goto, Transcutaneous pollinosis immunotherapy using a solid-in-oil nanodispersion system carrying T cell epitope peptide and R848, Bioengineering & Translational Medicine, 10.1002/btm2.10048, 2, 1, 102-108, 2017.04.
22. Zhigang Zhao, F. Kubota, Masahiro Goto, Development of novel adsorbent bearing aminocarbonylmethylglycine and its application to scandium separation, JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, 10.1002/jctb.4884, 91, 11, 2779-2784, 2016.11.
23. Water Yoshida, Masahiro Goto, Cu(II)-Imprinted Chitosan Derivative Containing Carboxyl Groups for the Selective Removal of Cu(II) from Aqueous Solution, JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 10.1252/jcej.15we293, 49, 7, 630-634, 2016.07.
24. Sivapragasam, Magaret, Moniruzzaman, Muhammad, Masahiro Goto, Recent advances in exploiting ionic liquids for biomolecules: Solubility, stability and applications, BIOTECHNOLOGY JOURNAL, 10.1002/biot.201500603, 11, 8, 1000-1013, 2016.08.
25. Tatsuo Maruyama, Masahiro Goto, Liquid-liquid extraction of enzymatically synthesized functional RNA oligonucleotides using reverse micelles with a DNA-surfactant, CHEMICAL COMMUNICATIONS, 10.1039/c6cc06985a, 52, 83, 12376-12379, 2016.12.
26. Lotfi, Meysam, Moniruzzaman, Muhammad, Masahiro Goto, Analysis of Multiple Solvation Interactions of Methotrexate and Ammonium Based Ionic Liquids Using COSMO-RS, PROCEEDING OF 4TH INTERNATIONAL CONFERENCE ON PROCESS ENGINEERING AND ADVANCED MATERIALS (ICPEAM 2016), 10.1016/j.proeng.2016.06.464, 148, 459-466, 2016.11.
27. Momoko Kitaoka, Rie Wakabayashi, Norah Kamiya, Masahiro Goto, Solid-in-oil nanodispersions for transdermal drug delivery systems, BIOTECHNOLOGY JOURNAL, 10.1002/biot.201600081, 11, 11, 1375-1385, 2016.11.
28. Yukio Hosomomi, Masahiro Goto, Diglycolic amic acid-modified E. coli as a biosorbent for the recovery of rare earth elements, BIOCHEMICAL ENGINEERING JOURNAL, 10.1016/j.bej.2016.06.005, 113, 102-106, 2016.09.
29. Yuzo Baba, Masahiro Goto, Mutual Separation of Indium, Gallium, and Zinc with the Amic Acid-type Extractant D2EHAG Containing Glycine and Amide Moieties, SOLVENT EXTRACTION RESEARCH AND DEVELOPMENT-JAPAN, 23, 9-18, 2016.05.
30. Adawiyah, Noorul, Moniruzzaman, Muhammad, Masahiro Goto, Ionic liquids as a potential tool for drug delivery systems, MEDCHEMCOMM, 10.1039/c6md00358c, 7, 10, 1881-1897, 2016.10.
31. Elgharbawy, Amal A., Moniruzzaman, Muhammad, Masahiro Goto, Ionic liquid pretreatment as emerging approaches for enhanced enzymatic hydrolysis of lignocellulosic biomass, BIOCHEMICAL ENGINEERING JOURNAL, 10.1016/j.bej.2016.01.021, 109, 252-267, 2016.05.
32. Masahiro Goto, Transcutaneous immunization against cancer using solid-in-oil nanodispersions, MEDCHEMCOMM, 10.1039/c5md00168d, 6, 7, 1387-1392, 2015.05, 特殊なナノコーティングによって、ガン抗原タンパク質をナノカプセル化し、担ガンマウスを用いて、ガンのワクチン効果を検証した。その結果、経皮投与によって、注射に匹敵するガンの抑制効果が観察された。.
33. 後藤 雅宏, Ionic liquid-mediated transcutaneous protein delivery with solid-in-oil nanodispersions, MEDCHEMCOMM, 10.1039/c5md00378d, 6, 12, 2124-2128, 2015.04.
34. Tatsuo Maruyama, Masahiro Goto, Cancer Cell Death Induced by the Intracellular Self-Assembly of an Enzyme-Responsive Supramolecular Gelator, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 10.1021/ja510156v, 137, 2, 770-775, 2015.04.
35. Zhigang Zhao, Masahiro Goto, Synergistic Extraction of Rare-Earth Metals and Separation of Scandium Using 2-Thenoyltriuoroacetone and Tri-n-octylphosphine Oxide in an Ionic Liquid System, JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 10.1252/jcej.14we360, 47, 8, 656-662, 2014.08.
36. Jian Yan, Masahiro Goto, Separation of Gold(III) in Acidic Chloride Solution Using Porous Polymeric Ionic Liquid Gel, JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 10.1252/jcej.14we162, 48, 3, 197-201, 2015.04.
37. Jian Yan, Masahiro Goto, Application of cellulose acetate to the selective adsorption and recovery of Au(III), CARBOHYDRATE POLYMERS, 10.1016/j.carbpol.2014.05.003, 111, 768-774, 2014.10.
38. Rie Wakabayashi, Masahiro Goto, The self-assembly and secondary structure of peptide amphiphiles determine the membrane permeation activity, RSC ADVANCES, 10.1039/c4ra02901a, 4, 58, 30654-30657, 2014.04.
39. Rie Wakabayashi, Masahiro Goto, A novel surface-coated nanocarrier for efficient encapsulation and delivery of camptothecin to cells, MEDCHEMCOMM, 10.1039/c4md00179f, 5, 10, 1515-1519, 2014.10.
40. Kojiro Shimojo, Masahiro Goto, Highly Efficient Extraction Separation of Lanthanides Using a Diglycolamic Acid Extractant, ANALYTICAL SCIENCES, 30, 2, 263-269, 2014.02.
41. Tatsuo Maruyama, Masahiro Goto, Selective adsorption and recovery of precious metal ions using protein-rich biomass as efficient adsorbents, PROCESS BIOCHEMISTRY, 10.1016/j.procbio.2014.02.016, 49, 5, 850-857, 2014.05.
42. Momoko Kitaoka, Masahiro Goto, Transdermal Immunization using Solid-in-oil Nanodispersion with CpG Oligodeoxynucleotide Adjuvants, PHARMACEUTICAL RESEARCH, 10.1007/s11095-014-1554-5, 32, 4, 1486-1492, 2015.04.
43. Tkahiko Kakoi, Masahiro Goto, Separation of Platinum and Palladium from Hydrochloric Acid Solutions with 1-Octyl-3-methylimidazolium Hexafluorophosphate as an Extractant, JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 10.1252/jcej.14we052, 47, 8, 666-670, 2014.08.
44. Hiromu Yoshiura, Noriho Kamiya, Masahiro Goto, Ionic Liquid-in-Oil Microemulsions as Potential Carrier for the Transdermal Delivery of Methotrexate, J. Chem. Eng. Japan, 46, 11, 794-796, 2013.11.
45. Yukiho Hosomomi, Fukiko Kubota, Masahiro Goto, Biosorption of Rare Earth Elements by Escherichia coli, J. Chem. Eng. Japan, 46, 7, 450-454, 2013.09.
46. Fan Yang, Fukiko Kubota, Masahiro Goto, A Comparative Study of Ionic Liquids and a Conventional Organic Solvent on the Extraction of Rare-earth Ions with TOPO, Solvent Extraction Research and Development, Japan, 20, 1, 225-232, 2013.05.
47. Fan Yang, Fukiko Kubota, Masahiro Goto, Extraction of Rare-Earth Ions with an 8-Hydroxyquinoline Derivative in an Ionic Liquid, Solvent Extraction Research and Development, Japan, 20, 1, 123-129, 2013.05.
48. Yuzo Baba, Fukiko Kubota, Masahiro Goto, Development of Novel Extractants with Amino Acid Structure for Efficient Separation of Nickel and Cobalt from Manganese Ions, INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH, 10.1021/ie403524a, 53, 2, 812-818, 2014.01.
49. 北岡 桃子, Kana Imamura, Noriho Kamiya, Masahiro Goto, Sucrose laurate-enhanced transcutaneous immunization with a solid-in-oil nanodispersion, MEDCHEMCOMM, 10.1039/c3md00164d, 5, 1, 20-24, 2014.01.
50. 北岡 桃子, Kana Imamura, Noriho Kamiya, Masahiro Goto, Needle-free immunization using a solid-in-oil nanodispersion enhanced by a skin-permeable oligoarginine peptide, INTERNATIONAL JOURNAL OF PHARMACEUTICS, 10.1016/j.ijpharm.2013.10.006, 458, 2, 334-339, 2013.12, 注射を不要とするワクチン投与技術野開発に成功した。.
51. Kojiro Shimojo, Fukiko Kubota, Masahiro Goto, Highly Efficient Extraction Separation of Lanthanides Using a Diglycolamic Acid Extractant, ANALYTICAL SCIENCES, 30, 2, 263-269, 2014.02.
52. Uju, Masahiro Goto, Noriho Kamiya, Low melting point pyridinium ionic liquid pretreatment for enhancing enzymatic saccharification of cellulosic biomass, BIORESOURCE TECHNOLOGY, 10.1016/j.biortech.2012.06.096, 135, 103-108, 2013.05.
53. Fan Yang, Fukiko Kubota, Masahiro Goto, Selective extraction and recovery of rare earth metals from phosphor powders in waste fluorescent lamps using an ionic liquid system, JOURNAL OF HAZARDOUS MATERIALS, 10.1016/j.jhazmat.2013.03.026, 254, 79-88, 2013.06.
54. Yoshiro Tahara, Noriho Kamiya, Masahiro Goto, Transdermal delivery of insulin using a solid-in-oil nanodispersion enhanced by arginine-rich peptides, MEDCHEMCOMM, 10.1039/c2md20059g, 3, 12, 1496-1499, 2012.12.
55. Fang Yang, Noriho Kamiya, Masahiro Goto, Transdermal delivery of the anti-rheumatic agent methotrexate using a solid-in-oil nanocarrier, EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, 10.1016/j.ejpb.2012.05.016, 82, 1, 158-163, 2012.09.
56. Masahiro Goto, Yoshiro Tahara, Noriho Kamiya, A novel double-coating carrier produced by solid-in-oil and solid-in-water nanodispersion technology for delivery of genes and proteins into cells, J. Controlled Release, 16, 713-721, 2012.08.
57. Fang Yang, Fukiko Kubota, Noriho Kamiya, Masahiro Goto, Extraction and Separation of Rare Earth Metal Ions with DODGAA in Ionic liquids, Solv. Extr. Res. Dev. Jpn,, 19, 1, 69-76, 2012.05.
58. K. Nose, Masahiro Goto, Yoshiki Katayama, Gold nanorods in an oil-base formulation for transdermal treatment of type 1 diabetes in mice, Nanoscale, 4, 3776-3780, 2012.06.
59. Masahiro Goto, Josui Shimada, Noriho Kamiya, Programmable protein protein conjugation via DNA-based self-assembly, Chem. Commun, 48, 6226-6228, 2012.05.
60. Masahiro Goto, Josui Shimada, Noriho Kamiya, Microplate assay for aptamer-based thrombin detection using a DNA-enzyme conjugate based on His-tag chemistry, Anal. Biochem., 421, 541-546, 2012.05.
61. Eiichi Toorisaka, Masahiro Goto, Intestinal patches with an immobilized solid-in-oil formulation for oral protein delivery, Acta Biomaterialia, 8, 653-658, 2012.04.
62. Y. Baba, F. Kubota, N. Kamiya, M. Goto, Selective Recovery of Dysprosium and Neodymium Ions by a Supported Liquid Membrane Based on Ionic Liquids, Solvent Extraction Research and Development, Japan, 18, 193-198, 2011.05.
63. F.Kubota, Y. Shimobori, Y. Baba, Y. Koyanagi, K. Shimojo, N. Kamiya, M. Goto, Application of ionic liquids to extraction separation of rare earth metals with an effective diglycol amic acid extractant, J. Chem. Eng. Japan, 51, 878-882, 2011.06.
64. J. Shimada, T. Maruyama, M. Kitaoka, N. Kamiya, M. Goto, DNA-enzyme conjugate with a weak inhibitor that can specifically detect thrombin in a homogeneous medium, Anal. Biochem., 414, 103-108, 2011.06.
65. D. Pissuwan, K. Nose, R. Kurihara, K. Kaneko, Y. Tahara, N. Kamiya, M. Goto, Y. Katayama, T. Niidome, A solid-in-oil dispersion of gold nanorods can enhance trandermal protein delivery and skin vaccination, Small, 7, 215-220, 2011.04.
66. M. Sakono, K. Motomura, T. Maruyama, N. Kamiya, M. Goto, Alpha casein micelles show not only molecular chaperone-like aggregation inhibition properties but also protein refolding activity from the denatured state, Biochem. Biophys. Res. Commu, 26, 494-497, 2011.04.
67. S. Egusa, T. Kitaoka, K. Igarashi, M. Samejima, M. Goto, H. Wariishi, Preparation and enzymatic behavior of surfactant-enveloped enzymes for glycosynthesis in nonaqueous aprotic media, Journal of Molecular Catalysis B: Enzymatic, 67, 225-230, 2010.12.
68. Y. Tahara, K. Namatsu, N. Kamiya, M. Hagimori, S. Kamiya, M. Arakawa, M. Goto, Transcutaneous immunization by a solid-in-oil nanodispersion, Chem. Comm., 33, 341-343, 2010.11.
69. M. Moniruzzaman, M. Tamura, Y. Tahara, N. Kamiya, M. Goto, Ionic liquid-in-oil microemulsion as a potential carrier of sparingly soluble drug: Characterization and cytotoxicity evaluation, Int. J. Pharm., 400, 243-250, 2010.10.
70. H. Abe, M. Goto, N. Kamiya, Enzymatic single-step preparation of multifunctional proteins, Chem. Commun., 33, 213-215, 2010.08.
71. M. Kitaoka, T. Wada, T. Nishio, M. Got, Fluorogenic Ribonuclease Protection (FRIP) Analysis of Single Nucleotide Polymorphisms (SNPs) in Japanese Rice (Oryza sativa L.) DNA for Cultivar Discrimination, Biosci. Biotech. Biochem., 45, 256-261, 2010.07.
72. Y. Okutani, S. Egusa, Y. Ogawa, T. Kitaoka, M. Goto, H. Wariishi, One-Step Lactosylation of Hydrophobic Alcohols by Nonaqueous Biocatalysis, Chem. Cat. Chem., 2, 950-952, 2010.06.
73. F. Kubota, Y. Shimobori, Y. Koyanagi, K. Shimojo, N. Kamiya, M. Goto, Uphill Transport of Rare Earth Metals through Highly Stable Supported Liquid Membrane Based on Ionic Liquid, Anal. Sci., 26, 289-290, 2010.05.
74. T. Niide, H. Shiraki, T. Oshima, Y. Baba, N. Kamiya, M. Goto, Quaternary ammonium bacterial cellulose for adsorption of proteins, Solvent Extr. Res. Dev., Jpn, 17, 73-81, 2010.05.
75. K. Sung, N. Kamiya, N. Kawata, S. Kamiya and M. Goto, Functional glass surface displaying a glutamyl donor substrate for transglutaminase-mediated protein immobilization, Biotechnol. J., 5, 456-462, 2010.04.
76. M. Moniruzzaman, Y. Tahara, M. Tamura, N. Kamiya and M. Goto, Ionic liquid-assisted transdermal delivery of sparingly soluble drugs, Chem. Commun.,, 46, 1452-1454, 2010.01.
77. D. Koda, T. Maruyama, N. Minakuchi, K. Nakashima, and M. Goto, Proteinase-mediated drastic morphological change of peptide-amphiphile to induce supramolecular hydrogelation, Chem. Commun. , 46, 978-981, 2010.01.
78. J. Okada, T. Maruyama, K. Motomura, K. Kuroki, K. Maenaka, M. Sakono, M. Goto, Enzyme-Mediated Protein Refolding, Chem. Comm., 2009, 7197-7199, 2010.05.
79. M. Kukisaki, M. Goto, A Comparative Study of SPG Membrane Emulsification in the Presence and Absence of Continuous-Phase Flow, J. Chem. Eng. Japan, 42, 7, 520-530, 2009.10.
80. K. Esaka, S. Yokota, S. Egusa, Y. Okutani, Y. Ogawa, T. Kitaoka, M. Goto, Preparation of Lactose-Modified Cellulose Films by a Nonaqueous Enzymatic Reaction and their Biofunctional Characteristics as a Scaffold for Cell Culture, Biomacromolecules, 10, 1265-1269, 2009.08.
81. F. Kubota, Y. Shimobori, Y. Koyanagi, K. Nakashima, K. Shimojo, N. Kamiya, M. Goto, Extraction Behavior of Indium with TOPO into Ionic Liquids, Solvent Extraction Research Development, Japan, 16, 142-146, 2009.07.
82. M. Kitaoka, H. Ichinose, M. Goto, Simultaneous visual detection of single-nucleotide variations in tuna DNA using DNA/RNA chimeric probes and ribonuclease A, Analytical Biochem., , 389, 6-11, 2009.07.
83. K. Minamihata, M. Tokunaga, N. Kamiya, S. Kiyoyama, H. Sakuraba, T. Ohshima, M. Goto, Development of a novel immobilization method for enzymes from hyperthermophiles, Biotechnology Letters, 21, 125, 128, 2009.06.
84. T. Mouri, T. Shimizu, N. Kamiya, H. Ichinose, M. Goto, Design of a cytochrome P450BM3 reaction system linked by two-step cofactor regeneration catalyzed by a soluble transhydrogenase and glycerol dehydrogenase, Biotechnol. Prog., , 25, 1372-1378, 2009.05.
85. K. Esaka, S. Yokota, S. Egusa, Y. Okutani, Y. Ogawa, T. Kitaoka, M. Goto, H. Wariishi,, Preparation of Lactose-Modified Cellulose Films by a Nonaqueous Enzymatic Reaction and their Biofunctional Characteristics as a Scaffold for Cell Culture, Biomacromolecules, 10, 1265-1269(2009), 2009.04.
86. M. Kitaoka, H. Ichinose, M. Goto, Simultaneous visual detection of single-nucleotide variations in tuna DNA using DNA/RNA chimeric probes and ribonuclease A, Analytical Biochem., 389, 6-11(2009) , 2009.04.
87. S. Egusa, S. Yokota, K. Tanaka, K. Esaki, Y. Okutani, Y. Ogawa, T. Kitaoka, M. Goto, H. Wariishi,, Surface modification of a solid-state cellulose matrix with lactose by a surfactant-enveloped enzyme in a nonaqueous medium, J. Mater. Chem., 19, 1836-1842(2009) , 2009.04.
88. K. Nakashima, N. Kamiya, D. Koda, T. Maruyama, M. Goto, Enzyme Encapsulation in Microparticles Composed of Polymerized Ionic Liquids for Highly Active and Reusable Biocatalysts, Org. Biomol. Chem., 35, 126-131(2009), 2009.04.
89. K. Nakashima, T. Maruyama, F. Kubota, M. Goto, Metal Extraction from Water and Organic Solvents into Fluorous Solvents by Fluorinated β-Diketone and Its Application to the Colorimetric Analysis of Metal Ions, Anal. Sci., 32, 241-245(2009) , 2009.01.
90. M.M. Zaman, N. Kamiya, and M. Goto, Biocatalysis in Water-in-Ionic Liquid Microemulsions: A Case Study with Horseradish  Peroxidase, Langmuir, 25, 977-982 (2009)., 2009.01.
91. Y. Tahara, S. Honda, N. Kamiya, H. Piao, A. Hirata, E. Hayakawa, T. Fujii, M. Goto, A solid-in-oil nanodispersion for transcutaneous protein delivery, J. Control. Release, 131, 14-18 (2008), 2008.12.
92. M. Kitaoka, N. Okamura, H. Ichinose, M. Goto, Detection of SNPs in Fish DNA: Application of the Fluorogenic Ribonuclease Protection (FRIP) Assay for the Authentication of Food Contents" , J. Agric. Food Chem., 56, 6246-6251 (2008), 2008.12.
93. S. Kiyoyama, T. Maruyama, N. Kamiya, M. Goto, Microcapsulation of DNA and the adsorption of toxic substances, J. Microencapsul., 25, 324-329 (2008), 2008.11.
94. M. Kukizaki, M. Goto, Demulsification of water-in-oil emulsions by permeation through Shirasu-porous-glass (SPG) membranes, J.Membr. Sci., 322, 196-203 (2008), 2008.10.
95. J. Shimada, T. Maruyama, T. Hosogi, J. Tominaga, N. Kamiya, M. Goto, Conjugation of DNA with protein using His-tag chemistry and its application to the aptamer-based detection system, Biotechnol. Lett., 30, 2001-2006 (2008), 2008.10.
96. F. Kubota, Y. Koyanagi, K. Nakashima, K. Shimojo, N. Kamiya, M. Goto, Extraction of Lanthanide Ions with an Organophosphorous Extractant into Ionic Liquids, Solvent Extr. Res. Dev, -Jpn., 15, 81-87 (2008), 2008.08.
97. T. Maruyama, H. Yamamura, M. Hiraki, Y. Kemori, H. Takata and M. Goto, Directed aggregation and fusion of lipid vesicles induced by DNA-surfactants, Colloids and Surfaces B: Biointerfaces, Biointerfaces, 66, 119-124 (2008), 2008.08.
98. H. Piao, N. Kamiya, A. Hirata, T. Fujii and M. Goto, A Nobel Solid-in-oil Nanosuspension for Transdermal Delivery of Diclofenac Sodium, Pharmaceutical Research., 25, 896-901 (2008)., 2008.08.
99. H. Yoshiura, Y. Tahara, M. Hashida, N. Kamiya, A. Hirata, T. Fujii and M. Goto, Design and in vivo evaluation of solid-in-oil suspension for oral delivery of human growth hormone, Biochemical Engineering Journal, 41, 106-110 (2008), 2008.07.
100. M. M. Zaman, N. Kamiya, K. Nakashima and M. Goto, Water-in-Ionic Liquid Microemulsions as a New Medium for Enzymatic Reactions, Green Chem., 10, 497-500 (2008), 2008.07.
101. Shiro Kiyoyama, Tatsuo Maruyama, Noriho Kamiya and Masahiro Goto, Immobilization of Proteins into Microcapsules and Their Adsorption Properties with respect to Precious-Metal Ions, Ind. Eng. Chem. Res., 47, 1527-1532 (2008), 2008.06.
102. Y. Tanaka, S. Doi, N. Kamiya, N. Kawata, S. Kamiya, K. Nakama, M. Goto, A chemically modified glass surface that facilitates transglutaminase-mediated protein immobilization, Biotechnol. Lett., 31, 1231-1236 (2008), 2008.05.
103. N. Kamiya, Y. Matsushita, M. Hanaki, K. Nakashima, M Narita, M. Goto and H. Takahashi, Enzymatic in situ Saccharification of Cellulose in Aqueous-Ionic Liquid Media, Biotechnol. Lett., 30, 1037-1040 (2008), 2008.04.
104. K. Nakashima, T. Maruyama, N. Kamiya and M. Goto, , Spectrophotometric Assay for Protease Activity in Ionic Liquids Using Chromogenic Substrates, Anal. Biochem., , 374, 285-290 (2008), 2008.03.
105. S. Hanioka, T. Maruyama, T. Sotani, M. Teramoto, H. Matsuyama, K. Nakashima, M. Hanaki, F. Kubota, M. Goto, , CO2 separation facilitated by task-specific ionic liquids using a supported liquid membrane, J. Membr. Sci. , 314, 1-4 (2008), 2008.02.
106. M. M. Zaman, N. Kamiya, K. Nakashima and M. Goto, Formation of Reverse Micelles in a Room-Temperature Ionic Liquid, ChemPhysChem. , 9, 689-692 (2008), 2008.01.
107. T. Oshima, B. Yoshinari, K. Shimojo, and M. Goto , Recognition of Lysine Residues on Protein Surfaces Using Calixarenes and Its Application. Curr. , Curr. Drug Discovery Tech., , 4, 220-228 (2007), 2007.12.
108. Muneharu GOTO, Hideaki KAWAKITA, Kazuya UEZU, Satoshi TUNEDA, Kyoichi SAITO, and Masahiro GOTO, , 'Design of Polymer Brushes for Immobilizing Enzymes onto Hollow Fiber Micropores in Organic Media Reaction', Biochem. Eng. J., , 37, 159-165 (2007), 2007.08.
109. Masato Kukizaki, Masahiro Goto, 'Preparation and characterization of a new asymmetric type of Shirasu porous glass (SPG) membrane used for membrane emulsification', JOURNAL OF MEMBRANE SCIENCE , 299 (1-2),190-199, 2007, 2007.08.
110. K. Shimojo, H. Naganawa, J. Noro, F. Kubota, and M. Goto, , Extraction Behavior and Separation of Lanthanides with a Diglycol Amic Acid Derivative and a Nitrogen-donor Ligand., , Anal. Sci., , 23, 1427-1430 (2007), 2007.05.
111. N.Kamiya, S.Doi, Y.Tanaka, H.Ichinose, M.Goto, , Functional immobilization of recombinant alkaline phosphatases bearing a glutamyl donor substrate peptide of microbial transglutaminase, , J. Biosci. Bioeng., , 104, 195-199 (2007), 2007.09.
112. T. Maruyama, T. Hosogi, M. Goto, Sequence-selective extraction of single-stranded DNA using DNA-functionalized reverse micelles. , Chem. Commun. , 4450 (2007) , 2007.08.
113. Hongyu Piao, Noriho Kamiya, Akihiko Hirata, Takeru Fujii and Masahiro Goto, 'A Novel Solid-in-oil Nanosuspension for Transdermal Delivery of Diclofenac Sodium' , Pharmaceutical Research, in press., 2007.10.
114. K. Shimojo, T. Oshima, H. Naganawa, M. Goto, "Calixarene-Assisted Protein Refolding via Liquid-Liquid Extraction", , Biomacromolecules,, 8, 3061-3066 (2007), 2007.10.
115. T. Maruyama, K. Nakashima, F. Kubota, M. Goto, Perfluorocarbon-based liquid-liquid extraction for separation of transition metal ions., , Anal. Sci., , 23, 763-765 (2007), 2007.07.
116. H. Naganawa, K. Shimojo, H. Mitamura, Y. Sugo, J. Noro, and M. Goto, A new "Green" Extractant of the Diglycol Amic Acid Type for Lanthanides, Solv. Extr. Res. & Devel. Japan, , 14, 151-160 (2007) , 2007.09.
117. F. Kubota, Y. Koyanagi, K. Nakashima, K. Shimojo, N. Kamiya and M. Goto, , Extraction of Cytochrome c by a Functionalized Ionic Liquid Containing a Crown Ether, Solv. Extr. Res. & Devel. Japan, , 14, 115-120 (2007), 2007.04.
118. M. Kukizaki, M. Goto, Preparation and evaluation of uniformly sized solid lipid microcapsules using membrane emulsification, COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS , 293(1-3), 87-94 (2007), 2007.05.
119. M. Kukizaki, M. Goto, Spontaneous formation behavior of uniform-sized microbubbles from Shirasu porous glass (SPG) membranes in the absence of water-phase flow. , COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS , 296(1-3) 174-181 (2007), 2007.05.
120. Yusuke Tanaka, Yukito Tsuruda, Motohiro Nishi, Noriho Kamiya, Masahiro Goto, 'Exploring Enzymatic Catalysis at a Solid Surface: A Case Study with Transglutaminase-Mediated Protein Immobilization.', Org. Biomol. Chem., , 5, 1764-1770 (2007), 2007.04.
121. J. Tominaga, N. Kamiya and M. Goto, , An enzyme-labeled protein polymer bearing pendant haptens. , Bioconjugate Chem. , 18, 860-865 (2007), 2007.05.
122. H. Yoshiura, M. Hashida, N. Kamiya, and M. Goto, , Factors affecting protein release behavior from surfactant-protein complexes under physiological conditions. , Int. J. Pharm. , 228, 654-658 (2007), 2007.06.
123. H. Piao, N. Kamiya, A. Hirata, H. Yokoyama, T. Fujii, I. Shimizu, S. Ito, M. Goto, , Reduction of gastric ulcerogenicity during multiple administration of diclofenac sodium by a novel solid-in-oil suspension. , Pharmaceutical Development and Technology, , 322, 312-316(2007), 2007.04.
124. T. Maruyama, H. Matsushita, Y. Shimada, I. Kamata, M. Hanaki, S. Sonokawa, N. Kamiya, M. Goto, Proteins and protein-rich biomass as environmental-friendly adsorbents selective for precious metal ions. , Environ. Sci. Technol. , 41(4),1359-1364 (2007), 2007.04.
125. J. Tominaga, Y. Kemori, Y. Tanaka, T. Maruyama, N. Kamiya and M. Goto, An enzymatic method for site-specific labeling of recombinant proteins with oligonucleotides. , Chem. Commun., 401-403 (2007), 2007.04.
126. T. Maruyama, C. Komatsu, J. Michizoe, S. Sakai and M. Goto, , Laccase-mediated degradation and reduction of toxicity of the postharvest fungicide imazalil. , Process Biochem., 42, 459-461 (2007), 2007.03.
127. S. Egusa, T. Kitaoka, M. Goto, H. Wariishi, Synthesis of Cellulose In Vitro by Using a Cellulase/Surfactant Complex in a Nonaqueous Mediumc, Angew. , Chem. Int. Edit., 46(12), 2063-2065, (2007), 2007.03.
128. J. Tominaga, N. Kamiya and M. Goto, An enzyme-labeled protein polymer bearing pendant haptens. , Bioconjugate Chem, 18, 860-865 (2007), 2007.03.
129. T. Maruyama, H. Matsushita, Y. Shimada, I. Kamata, M. Hanaki, S. Sonokawa, N. Kamiya, M. Goto, , Proteins and protein-rich biomass as environmental-friendly adsorbents selective for precious metal ions. , Environ. Sci. Technol. , 41, 1356-1364 (2007), 2007.03.
130. J. Tominaga, Y. Kemori, Y. Tanaka, T. Maruyama, N. Kamiya and M. Goto, An enzymatic method for site-specific labeling of recombinant proteins with oligonucleotides. , Chem. Commun., 401 - 403 (2007), 2007.03.
131. T. Maruyama, S. Sonokawa, H. Matsushita and M. Goto, Inhibitiory effects of gold(III) ions on ribonuclease and deoxyribonuclease. , J. Inorg. Biochem. , 101, 180-186 (2007), 2007.02.
132. T. Maruyama, C. Komatsu, J. Michizoe, S. Sakai and M. Goto, Laccase-mediated degradation and reduction of toxicity of the postharvest fungicide imazalil. , Process Biochem, 42, 459-461 (2007), 2007.01.
133. N. Othman, H. Mat, M. Goto, Separation of silver from photographic wastes by emulsion liquid membrane system. , JOURNAL OF MEMBRANE SCIENCE , 282, 171-177 (2006), 2006.11.
134. K. Shimojo, N. Kamiya, F. Tani, H. Naganawa, Y. Naruta and M. Goto, Extractive Solubilization, Structural Change, and Functional Conversion of Cytochrome c in Ionic Liquids via Crown Ether Complexation. , Anal. Chem., 78, 7735-7742 (2006), 2006.11.
135. M. Kukizaki, M. Goto, Size control of nanobubbles generated from Shirasu-porous-glass (SPG) membranes. , J. Membr. Sci, 281, 386-396 (2006), 2006.12.
136. M. Kukizaki, and M. Goto, Effects of Interfacial Tension and Viscosities of Oil and Water Phases on Monodispersed Droplet Formation Using a Shirasu-Porous-Glass (SPG) Membrane., Membrane, 31, 215-220 (2006), 2006.12.
137. K. Nakashima, T. Maruyama, N. Kamiya, M. Goto, Homogeneous Enzymatic Reactions in Ionic Liquids with Poly(ethylene glycol)-Modified Subtilisin. , Org. Biomol. Chem, 4, 3462-3467 (2006). , 2006.09.
138. M Goto, H Kawakita, K Uezu, S Tsuneda, K Saito, M Goto, M Tamada, T Sugo, Esterification of Lauric Acid Using Lipase Immobilized in the Micropores of a Hollow-Fiber Membrane. , J Amer Oil Chemists' Soc, , 83, 209-213 (2006), 2006.08.
139. K. Nakashima, J. Okada, T. Maruyama, N. Kamiya, M. Goto, Activation of lipase in ionic liquids by modification with comb-shaped poly(ethylene glycol), Sci. Technol. Adv. Mater, 7, 692-698 (2006), 2006.10.
140. T Mouri, N Kamiya, M Goto, Increasing the catalytic performance of a whole cell biocatalyst harboring a cytochrome P450cam system by stabilization of an electron transfer component. , Biotechnol. Lett, 28, 1509-1513 (2006), 2006.07.
141. N. Othman, C-K. Hie, M. Goto, H. Mat, Emulsion liquid membrane extraction of silver from photographic waste using CYANEX 302 as the mobile carrier. , Solv. Extr. Res. Dev. Jpn, 13, 191-202 (2006). , 2006.06.
142. M. Shinshi, Takayasu Sugihara, T. Osakai, M. Goto, Electrochemical Extraction of Proteins by Reverse Micelle Formation. , Langmuir, 22, 5937-5944 (2006). , 2006.07.
143. F. Kubota, H. Yamada, M. Goto, S. Furusaki, Extraction of adenosine nucleotides by a long-chain quaternary ammonium salt, Solv. Extr. Res. Dev. Jpn, 13, 107-114 (2006). , 2006.05.
144. H. Piao, N Kamiya, J Watanabe, H Yokoyama, A Hirata, T Fujii, I Shimizu, S Ito, M Goto, Oral delivery of diclofenac sodium using a novel solid-in-oil suspension, Int. J. Pharm, 313, 159-162 (2006), 2006.06.
145. K. Shimojo, H. Naganawa, F. Kubota, M. Goto, Solvent Extraction of Lanthanides into an Ionic Liquid Containing N, N, N', N'-tetrakis(2-pyridylmethyl)ethylenediamine. , Anal Biochem , 35, 484-485 (2006)., 2006.06.
146. T. Maruyama, T. Shinohara, T. Hosoki, H. Ichinose, N. Kamiya and M. Goto, Masking oligonucleotides improve sensitivity of mutation detection based on guanine quenching. , Anal Biochem , 354, 8-14 (2006). , 2006.05.
147. T. Ono, M. Goto, Peroxidative catalytic behavior of cytochrome c solubilized in reverse micelles, Biochem. Eng. J., 28, 156-160 (2006)., 2006.05.
148. T. Maruyama, C. Komatsu, J. Michizoe, H. Ichinose and M. Goto, Laccase-mediated oxidative degradation of the herbicide dymron. , Biotechnol Prog, 22, 426-430 (2006). , 2006.05.
149. T. Mouri, J. Michizoe, H. Ichinose, N. Kamiya, and M. Goto, A recombinant Escherichia coli whole cell biocatalyst harboring a cytochrome P450cam monooxygenase system coupled with enzymatic cofactor regeneration. , Appl. Microbiol. biotechnol, 72, 514-520 (2006), 2006.04.
150. K. Shimojo, K. Nakashima, N. Kamiya, M. Goto, Crown Ether-Mediated Extraction and Functional Conversion of Cytochrome c in Ionic Liquids, Biomacromolecules, Vol.7, 2-6 (2006), 2006.04.
151. H Ichinose, M Kitaoka, N Okamura, T Maruyama, N Kamiya, M Goto, Detection of single-base mutations by fluorogenic ribonuclease protection (FRAP) assay, Anal Chem, 10.1021/ac050782k, 77, 21, 7047-7053, 77, 7047-7053 (2005), 2005.10.
152. T. Oshima, H. Higuchi, K. Ohto, K. Inoue, M. Goto, Selective Extraction and Recovery of Cytochrome c by Liquid-Liquid Extraction Using a Calix[6]arene Carboxylic Acid Derivative, Langmuir,, 10.1021/la050364a, 21, 16, 7280-7284, 21, 7280-7284 (2005), 2005.09.
153. K. Nakashima, T. Maruyama, N. Kamiya, M. Goto, Comb-shaped poly(ethylene glycol)-modified subtilisin Carlsberg is soluble and highly active in ionic liquids, Chem. Commun, 10.1039/b505479f, 34, 4297-4299, 4297-4299 (2005), 2005.09.
154. E. Miyako, T. Maruyama, N. Kamiya, M. Goto, A supported liquid membrane encapsulating a surfactant-lipase complex for selective separation of organic acids, Chem Eur J, 10.1002/chem.200400691, 11, 4, 1163-1170, 11, 1163-1170 (2005)., 2005.01.
155. E. Miyako, T. Maruyama, N. Kamiya, and M. Goto, Enantioselective transport of (S)-ibuprofen through a lipase-facilitated supported liquid membrane based on ionic liquids, Chem Commun, 2926-2927 (2003)., 2003.12.
156. E. Toorisaka, H. Ono, K. Arimori, N. Kamiya, M. Goto, Hypoglycemic effect of surfactant-coated insulin solubilized in a novel solid-in-oil-in-water (S/O/W) emulsion, Int. J. Pharm, 10.1016/S0378-5173(02)00674-9, 252, 1-2, 271-274, 252, 271-274 (2003), 2003.12.
157. L.C. Park, T. Maruyama, M. Goto, DNA hybridization in reverse micelles and its application to mutation detection, Analyst, 10.1039/b208755n, 128, 2, 161-165, 128, 161-165 (2003)., 2003.06.
158. Masahiro Goto, Fumiyuki Nakashio, Development of new surfactants for liquid surfactant membrane process, J. Chem. Eng. Japan, 10.1252/jcej.20.157, 20, 2, 157-164, Vol. 20, No. 2, 157 - 164, 1987.06.
159. Development of Microbioreactor for biodegradation of organic pollutants.
Works, Software and Database
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Presentations
1. Kozaka Shuto, Nakata Takahiro, Akaki Junji, Tajima Shiro, Kunitomo Fiji, Goto Masahiro, Utilization of reverse micelles for skin permeation enhancement of bioactive macromolecules, The 30th ISChE, 2017.12.
2. R. M. Moshikur, R. Wakabayashi, Y. Tahara, M. Moniruzzaman, N. Kamiya, M. Goto, Salicylate amino acid esters as the novel Active Pharmaceutical Ingredient Ionic Liquids (API-ILs): characterization and cytotoxicity evaluation, 10th AFOB Regional Symposium 2018, 2018.01.
3. M. R. Chowdhury, R. Wakabayashi, Y. Tahara, M. Moniruzzaman, N. Kamiya, M.Goto, Ionic Liquids Based Paclitaxel IV Injection: A New Potential Formulation for Cancer Treatment, 10th AFOB Regional Symposium 2018, 2018.01.
4. Masahiro Goto, Novel Cancer Vaccine by Transcutaneous Drug Delivery System Using Solid-in-Oil Nano Carrier, 10th AFOB Regional Symposium 2018, 2018.01.
5. R. Wakabayashi, M. Katsuya, N. Kamiya, M. Goto, Multi-block fibrous assembly of peptide amphiphiles based on intrinsic immiscibility, The Second International Symposium on Biofunctional Chemistry (ISBC2017), 2017.12.
6. H. Kouno, Y. Tahara, R. Wakabayashi, N. Kamiya, M. Goto, Development of transcutaneous cancer vaccine by oil-based nanodispersion technique, The 30th International Symposium on Chemical Engineering, 2017.12.
7. Aiko Yamada, Rie Wakabayashi, Yoshiro Tahara, Noriho Kamiya, Masahiro Goto, Solid-in-Oil nanodispersion with an amino acid as a permeation enhancer for transcutaneous vaccine, The 30th International Symposium on Chemical Engineering, 2017.12.
8. R. Kono, W. Yoshida, F. Kubota, M. Goto, Separation of Au(III) from mobile phones using a polymer inclusion membrane with D2EHAG as the carrier, The 30th International Symposium on Chemical Engineering, 2017.12.
9. W. Yoshida, Y. Baba, F. Kubota, S.D. Kolev, M. Goto, Membrane transport of critical metal ions and stability studies of polymer inclusion membranes, The 30th International Symposium on Chemical Engineering, 2017.12.
10. R. Kono, W. Yoshida, F. Kubota, M. Goto, Selective extraction and recovery of precious metals with a novel amic acid extractant from waste mobile phones, The 21st International Solvent Extraction Conference(ISEC2017), 2017.11.
11. M. L. Firmansyah, F. Kubota, M. Goto, Effective separation of Pt(IV), Pd(II), and Rh(III) in acidic solution by using phosphonium-based ionic liquids, The 21st International Solvent Extraction Conference(ISEC2017), 2017.11.
12. M. Sharif, W. Yoshida, F. Kubota, M. Goto, Selective extraction of scandium from other REEs using binary extractant of PC-88A and versatic10 from nitrate media, The 21st International Solvent Extraction Conference(ISEC2017), 2017.11.
13. W. Yoshida, Y. Baba, F. Kubota, S.D. Kolev, M. Goto, Separation of scandium(III) from lanthanides using a polymer inclusion membrane containing an amic acid-extrctant carrier, The 21st International Solvent Extraction Conference, 2017.11.
14. W. Yoshida, Y. Baba, F. Kubota, S.D. Kolev, M. Goto, Selective membrane transport of rare earth ions by a polymer inclusion membrane, The 17th Congress of the Asian Pacific Confederation of Chemical Engineering, 2017.08.
15. 後藤 雅宏, 溶媒抽出プロセスに実利用可能な新規抽出剤の開発とその課題, 資源・素材&EARTH 2017, 2017.09.
16. Masahiro Goto, Cancer Vaccine by Transcutaneous Antigen-Peptide Delivery
Using Solid-in-Oil Technique
, APCChE 2017, 2017.08.
17. Masahiro Goto, TRANSDERMAL CANCER VACCINE BY SOLID-IN-OIL(S/O) NANODISPERSIONS, ACB 2017, 2017.07.
18. Qingliang Kong, Masahiro Goto, Transcutaneous immunotherapy for pollinosis using Solid-in-Oil Nanodispersion, 9th AFOB Regional Symposium, 2017.02.
19. Masahiro Goto, TRANSDERMAL CANCER VACCINE BY SOLID-IN-OIL(S/O) NANODISPERSIONS, 9th AFOB Regional Symposium, 2017.02.
20. Masato Sakuragi, Masahiro Goto, Solid-in-oil nanodispersions for transcutaneous cancer vaccine by induction of antitumor immunity against melanoma, The 29th International Symposium on Chemical Engineering, 2016.12.
21. Qingliang Kong, Masahiro Goto, Development of a transcutaneous immunotherapy for Japanese Cedar Pollinosis using Solid-in-Oil nanodispersions, The 29th International Symposium on Chemical Engineering, 2016.12.
22. Water Yoshida, Masahiro Goto, Development of polymer inclusion membranes containing an amid acid-type carrier for platinum group metal separation, The 29th International Symposium on Chemical Engineering, 2016.12.
23. Atsushi Oka, Masahiro Goto, Transcutaneous immunotherapy of Japanese ceder pollinosis using CpG as an adjuvant, The 29th International Symposium on Chemical Engineering, 2016.12.
24. Kouno Hideto, Masahiro Goto, Development of transcutaneous cancer vaccine by oil based nano dispersion technique, The 29th International symposium on Chemical Engineering, 2016.12.
25. Riho Kouno, Masahiro Goto, Recovery of precious metals from leach liquor of waste mobile phones with a novel amic acid extractant, The 29th International symposium on Chemical Engineering, 2016.12.
26. Rie Wakabayashi, Masahiro Goto, Enzyme-Reactive Self-Assembling Peptides for Biomacromolecular Functionalization, AIChE 2016 annual meeting, 2016.11.
27. Masahiro Goto, Solid-in-Oil (S/O) Nanodispersions for Transdermal Cancer Immunotherapy, AIChE 2016 annual meeting,, 2016.11.
28. Atsushi Oka, Masahiro Goto, Transcutaneous immunotherapy of Japanese ceder pollinosis with CpG as an adjuvant, The 22nd Symposium of Young Asian Biological Engineers’ Community, 2016.10.
29. Hidenao Kono, Masahiro Goto, Development of transcutaneous cancer vaccine by Solid-in-Oil technology, The 22nd Symposium of Young Asian Biological Engineers’ Community, 2016.10.
30. Qingliang Kong, Masahiro Goto, Development of Transcutaneous Pollinosis Immunotherapy by Solid-in-Oil technology, The 22nd Symposium of Young Asian Biological Engineers’ Community, 2016.10.
31. Qingliang Kong, Masahiro Goto, Transdermal pollinosis immunotherapy by Solid-in-Oil nanodispersions,, 化学工学会第48回秋季大会, 2016.09.
32. Masahiro Goto, Commercialization of Nano-capsules for Transdermal Drug Delivery Systems, Asian Federation of Biotechnology(AFOB) Summer Forum, 2016.08.
33. Masahiro Goto, Selective separation and recovery of strategically important metals using a polymer inclusion membrane containing an acidic extractant with alkylamide and amino acid moieties, IEX 2016: Ion Exchange, 2016.07.
34. Masahiro Goto, Challenge of Commercialization of Transdermal Drug Delivery Systems Invented in University, International Symposium on Biotechnology and Bioengineering, 2016.06.
35. Masahiro Goto, Challenge of Commercialization of Surfactant-Coating Nano Capsules Invented in University, International Colloid & Surfaces Symposium in Asia, 2016.06.
36. Masahiro Goto, New Extractants Applicable to Industrial Solvent Extraction Process for Rare Earth Separation, International Rare Earths Conference 2016, 2016.06.
37. Masahiro Goto, Activation of Biocatalysts in Ionic Liquids as a green reaction medium, International Catalysis Conference 2016, 2016.05.
38. Masahiro Goto, Cancer Immunotherapy by Transdermal Drug Delivery Systems Using Nano Coating Drug Carrier, ICES Seminar, 2016.05.
39. Rie Wakabayashi, Masahiro Goto, Enzyme-mediated assembly of biomolecules on a designer scaffold based on self-assembled peptides, The International Chemical Congress of Pacific Basin Societies 2015, 2015.12.
40. Masahiro Goto, New extractants applicable to industrial solvent extraction for the recovery of strategic metals, PacifChem 2015, 2015.12.
41. Wataru Yoshida, Masahiro Goto, Development of new extractant applicable to industrial solvent extraction for the recovery of precious metals, The 27th International Symposium on Chemical Engineering, 2015.12.
42. Yukiho Hosomomi, Masahiro Goto, Chemical modification of E. Coli and its application to the biosorption of metal ions, The 27th International Symposium on Chemical Engineering, 2015.12.
43. Oueng Kong, Masahiro Goto, Development of a transcutaneous pollinosis immunotherapy by using a solid-in-oil nanodispersion, The 28th International Symposium on Chemical Engineering, 2015.12.
44. Ayaka Suehiro, Masahiro Goto, Supramolecular Peptide Scaffold fr an Enzymatic Assembly of Functional Molecules, The 28th International Symposium on Chemical Engineering, 2015.12.
45. Takafumi Saeki, Masahiro Goto, Enzymatic assembly of proteins onto synthetic polymer scaffold, The 28th International Symposium on Chemical Engineering, 2015.12.
46. Takuji Kawakami, Masahiro Goto, Enzymatic Strategy for Lipidization of Functional Proteins, The 28th International Symposium on Chemical Engineering, 2015.12.
47. Masahiro Goto, Transdermal Cancer Immunization by a Surfactant-Coated Antigen Nanocarrier, ACB 2015, 2015.11.
48. Masahiro Goto, Transdermal Cancer Vaccine Using Antigen-Coating Nano Drug Carrier, AIChE 2015, 2015.11.
49. Wataru Yoshida, 後藤 雅宏, Extraction performance of novel amino acid derivative extractant for precious metal ions, 2015 Taiwan/Korea/Japan Joint Meeting on Chemical Engineering, 2015.11.
50. Yukiho Hosomomi, Masahiro Goto, Modification of E-coli cell surface for preparing a metal ion adsorbent, 2015 Taiwan/Korea/Japan Joint Meeting on Chemical Engineering, 2015.11.
51. Masato Sakuragi, Masahiro Goto, The melanoma prevention by transcutaneous cancer vaccine using Solid-in-Oil Technique, 2015 Taiwan/Korea/Japan Joint Meeting on Chemical Engineering, 2015.11.
52. Shota Araki, Masahiro Goto, Transdermal delivery of a vaccine antigen by the addition of ionic liquid, 2015 Taiwan/Korea/Japan Joint Meeting on Chemical Engineering, 2015.11.
53. Masahiro Goto, Ayaka Naritomi, Transcutaneous immunization using Solid-in-Oil nanodispersion with immunostimulatory adjuvants, 2015 Taiwan/Korea/Japan Joint Meeting on Chemical Engineering, 2015.11.
54. Masahiro Goto, Yukiho Hosomomi, Biosorption of rare earth elements by chemically modified E. coli, YABEC 2015, 2015.10.
55. Masahiro Goto, New Extractants Applicable to Industrial Solvent Extraction Processes for Critical Metal Separation, APCChE 2015, 2015.09.
56. 吉田航, 後藤 雅宏, Extraction of precious metals using novel amic acid-type extractant, 3rd International Symposium on Host Compounds for Separation and Functionality, 2015.07.
57. Masahiro Goto, Biocatalysis in Ionic Liquids, Southeast Asia Catalysis Conference 2015, 2015.05.
58. Masahiro Goto, Challenge to Commercialization of Advanced Drug Delivery Technology Developed in University, ICES Seminar, 2015.05.
59. Yoko Shin, Masahiro Goto, Development of transcutaneous pollinosis immunotherapy by using a solid-in-oil technique, The 27st International Symposium on Chemical Engineering, 2014.12.
60. Aya Naritomi, Masahiro Goto, Development of transcutaneous vaccine by Solid-in-Oil technology, The 27st International Symposium on Chemical Engineering, 2014.12.
61. Yuzo Baba, R.W. Cattrall, S.D. Kolev, Masahiro Goto, Development of polymer inclusion membrane with amide acid type extractant for separation of critical metals, 6th Int. Conf. Ion Exch., 2014 (ICIE 2014), 2014.11.
62. Yukiho Hosomomi, Masahiro Goto, Sorption properties of rare earth elements on chemically modified E. coli, 6th Int. Conf. Ion Exch., 2014 (ICIE 2014), 2014.11.
63. Yuzo Baba, R.W. Cattrall, S.D. Kolev, Masahiro Goto, Highly effective separation of scandium using a polymer inclusion membrane with an amide acid-type carrier, 10th Int. conf. Sep. Sci. Technol., 2014 (ICSST 14), 2014.11.
64. Masahiro GOTO, New extractants applicable to industrial solvent extraction process for rare metal separation, Int. Solv. Extrn. Cof., 2014, 2014.09.
65. Fukiko Kubota, Masahiro GOTO, Separation of rare earth metal ions by synergistic system with 2-thenoyltrifluoroacetone and TOPO, ISEC2014, 2014.09.
66. Yuzo Baba, Masahiro Goto, Effective Separation and Recovery of Rare Earth Metals by Using Supported Ionic Liquid Membrane System, The 8th International Membrane Science & Technology Conference (IMSTEC2013), 2013.12.
67. Yuya Hirakawa, Masahiro Goto, Development of transdermal cancer immunotherapy by using Solid-in-Oil nanodispersion
, The 26th International Symposium on Chemical Engineering, 2013.12.
68. Yukiho Hosomomi, Masahiro Goto, Chemical modification of E. coli for recovery of rare earth elements, 26th Int. Symp. Chem. Eng., Daejeon/Chungnam-Kyushu(Japan), 2013.12.
69. Masahiro Goto, Yusuke Tanaka, Transdermal delivery of salbutamol sulfate using a solid-in-oil nanodispersion, 26th Int. Symp. Chem. Eng., Daejeon/Chungnam-Kyushu(Japan), 2013.12.
70. Yoko Shin, Masahiro Goto, Transdermal pollinosis immunotherapy by a solid-in-oil nanodispersion, The 26th International Symposium on Chemical Engineering (ISChE 2013), 2013.12.
71. Masahiro Goto, Recycling of Rare Earth Metals by a Ionic Liquid Membrane, Critical Metal Recycle Symposium, 2013.11.
72. Masahiro Goto, Challenge to Commercialization of Advanced Technology in University, Japan-Korea-Taiwan Joint Chemical Engineering Symp., 2013.11.
73. Masahiro Goto, Directed Aggregation and Fusion of vesicles induced by a DNA surfactant, AIChE Annual Meeting, 2013,, 2013.11.
74. Masahiro Goto, Nanodispersion of Pharmaceutical Ingredients for Transdermal Drug Delivery Systems, INCHEM TOKYO, 2013.10.
75. Rie Wakabayashi, Masahiro Goto, Nanostructured Peptide Amphiphiles for Supramolecular Biomaterials, New Trends of Nano- or Bio-Materials Design in Supramolecular Chemistry (2013 NNBS), 2013.09.
76. Yukiho Hosomomi, Masahiro Goto, Development of bacterial biosorbent for rare earth elements, International Symposium on Cell Surface Structures and Functions, 2013.09.
77. Jian Yang, Masahiro Goto, Development of Membrane Separation System for Rare Metals Based on Ionic Liquids, Conference of Aseanian Membrane Society(AMS8), 2013.07.
78. Fukiko Kubota, Masahiro Goto, Selective Transport of Rare Earth Metals through a Supported Liquid Membrane Using Ionic Liquids, Conference of Aseanian Membrane Society(AMS8), 2013.07.
79. Yuko Abe, Noriho Kamiya, Masahiro Goto, Development of pH-sensitive double coating carrier for intracellular drug delivery
, The 25th International Symposium on Chemical Engineering, 2012.12.
80. Jian Yang, Fukiko Kubota, Masahiro Goto, Separation of precious metals by using ion-exchange polymers, The 25th International Symposium on Chemical Engineering, 2012.12.
81. Ryutaro Ishiyama, Masahiro Goto, A novel double-coating carrier for cancer-targeted drug delivery, The 25th International Symposium on Chemical Engineering, 2012.12.
82. Kana Imamura, Noriho Kamiya, Masahiro Goto, Development of transcutaneous vaccine formulation using Solid-in-Oil technology, The 25th International Symposium on Chemical Engineering, 2012.12.
83. Yusuke Tanaka, Masahiro Goto, Transdermal delivery of salbutamol sulfate by a solid-in-oil nanodispersion, The 25th International Symposium on Chemical Engineering, 2012.12.
84. Yukiho Hosomomi, Fukiko Kubota, Masahiro Goto, Biosorption of rare earth elements onto E. coli, The 25th International Symposium on Chemical Engineering, 2012.12.
85. Ionic Liquids and Bioscience .
86. Separation of Rare Earth Metals by Using Ionic Liquids.
Membership in Academic Society
  • SCEJ
  • ACS
  • AIChE
Awards
  • Molecular Design of Novel Extractants that show high selectivity to Rare Earth Metals
  • YABEC Award 2011
  • Development of new surfactants for liquid membranes
Educational
Educational Activities
Functional Material Processing
Bioprocess Desigh
Biochemical engineering
Other Educational Activities
  • 2010.04.
  • 2009.05.
  • 2009.02.
Social
Professional and Outreach Activities
Joint research
MIT
Prof. T Alan Hatton.