Kyushu University Academic Staff Educational and Research Activities Database
List of Papers
Akinori Hirashima Last modified date:2019.03.01

Associate Professor / Division of Molecular Biosciences / Department of Bioscience and Biotechnology / Faculty of Agriculture


Papers
1. Oany, A.R., Mia, M., Pervin, T., Hasan, Md.N. and Hirashima, A., Identification of potential drug targets and inhibitor of the pathogenic bacteria Shigella flexneri 2a through the subtractive genomic approach., In Silico Pharmacol. , 6, 11, 2018.06, [URL].
2. Md. Nazmul Hasan, Mohammad Jakir Hosen, Prasoon Kumar Thakur, Ruhshan Ahmed Abir, Abdullah Zubaer, Guo Renkai, Mayumi Yoshida, Hiroto Ohta, 李 在萬, 日下部 宜宏, 平島 明法, In vitro screening for inhibitor of cloned Drosophila melanogaster tyramine-β-hydroxylase and docking studies, International Journal of Biological Macromolecules, 93, 889-895, 2016.12, Biogenic amines are common biologically active substances extended within the whole animal kingdom where they play vital roles as signal transducer as well as regulator of cell functions. One of these biogenic amines called octopamine (OA) is synthesized from tyramine (TA) by the catalysis of tyramine-β-hydroxylase (TβH) originated in the insect nervous system. Both TA and OA act as neurotransmitters, neurohormones and neuromodulators in the arthropod nervous system. Herein, the inhibitory activity of 1-arylimidazole-2(3H)-thiones (AITs) was tested on cloned Drosophila tyramine-β-hydroxylase (DmTβH) expressed in Bombyx mori strain. Radiolabelled 3H-TA was used to analyze the activity of AITs exhibited inhibitory effects on DmTβH, whose ID50 values range from 0.02 to 2511 nM where DmTβH was inhibited in a dose-dependent manner at pH 7.6 and 25 °C during a 30 min of incubation. To understand the catalytic role of the TβH, a three dimensional structure of the TβH from Drosophila melanogaster was constructed by homology modeling using the Phyre2 web server with 100% confidence. The modeled three-dimensional structure of TβH was used to perform the docking study with AITs. This may give more insights to precise design of inhibitors for TβH to control insect’s population..
3. Md. Nazmul Hasan, Nur Kabidul Azam, Md. Nasir Ahmed, 平島 明法, A randomized ethnomedicinal survey of snakebite treatment in southwestern parts of Bangladesh, Journal of Traditional and Complementary Medicine, 6, 4, 337-342, 2016.10, Snakebite is the single most important toxin-related injury, causing substantial mortality in many parts of the Africa, Asia and the Americas. Incidence of snakebite is usually recorded in young people engaged in active physical work in rural areas. The various plant parts used to treat snakebite included whole plant, leaves, barks, roots and seeds. Most bites in Bangladesh are recorded between May and October with highest number in June. Lower and upper limbs are most common sites of snakebite, but it may happen in other sites as well. Snake venom has been the cause of innumerable deaths worldwide. However, antiserum does not provide enough protection against venom induced haemorrhage, necrosis, nephrotoxicity and hypersensitivity reactions. Informed consent was obtained from the practitioners prior to interviews. After the survey, it is concluded that the medicinal plants used by tribal medicinal practitioners in Bangladesh for treatment against snakebite are Acyranthes aspera L., Amaranthus Viridis L., Asparagus racemosus Wild and Emblica officinalis Gaertn, while the non-tribal communities used 35 plant species among them, most of the plants reported as new species used against snakebite in the belonging family. The plants present a considerable potential for discovery of novel compounds with fewer side effects for treatment of antisnake venom and can, at least in Bangladesh, become a source of affordable and more easily available drugs.
4. Md. Nazmul Hasan, Arafat Rahman Oany, 平島 明法, Comparative structure analysis of tyramine-β-hydroxylase from fruit fly and ADME/T-based profiling of 1-arylimidazole-2(3H)- thiones as potential inhibitors, Int. J. Anal. Bio-Sci., 4, 3, 37-45, 2016.10, Tyramine-β-hydroxylase (TβH) converts tyramine to octopamine, a neurotransmitter
present in insects similar to norepinephrine in mammals. The amino acid sequence of this enzyme
is homologous to that of dopamine-β-hydroxylase (DβH). The amino acids sequence of TβH from
Drosophila melanogaster (DmTβH) is highly similar (56.3%) with African malaria mosquito
Anopheles gambiae whereas 41.9% protein similarity was found with human DβH. In this study,
the 3D structure of DmTβH was predicted using 2 homology based predpiction servers:
MODELLER and Phyre2. The homology structures were evaluated and stereo-chemical analyses
were done by Ramachandan plot analysis. Validation of these homology models by Ramachandan
plot revealed that MODELLER generated model was comparatively better than that of the Phyre2.
Additionally. It was found that 1-[4-(trifluromethyl)phenyl]-1,3-dihydro-2H-imidazole-2-thione
binds to the protein with a binding energy value -10.4 (Kcal/mol) and showed a drug likeness
activity with no predicted health hazards. It can be used as a potent inhibitor of DmTβH to design
effective pesticide in future after advanced evaluation..
5. Hasan, Md. N., Munshi, M., Rahman, M. H., Alam, S.M.N., 平島 明法, Evaluation of antihperglycemic activity of Lasia spinosa leaf extracts in Swiss albino mice, World J. Pharm. Pharmaceut. Sciences , 3, 10, 118-124, 3(10), 118-124, 2014.10.
6. Hasan, Md. N., Rahman, Md. H., Guo, R., 平島 明法, Hypoglycemic activity of methanolic leaf extract of Blumea lacera in Swiss-albino mice, Asian Pac. J. Trop. Dis. , 10.1016/S2222-1808(14)60652-6, 5, 3 , 195-198, 2015.03.
7. Ahmed Ali, Nazmul Hasan, Renkai Guo, Hiroto Ohta, Akinori Hirashima, Molecular cloning and characterization of a recombinant Bombyx mori tyramine-β-hydroxylase in silkworm cell line using baculovirus expression vector system., J. Asia-Pac. Entomol. 17(3), 221-227, 17, 3, 221-227, 2014.02, Octopamine (OA) and tyramine (TA) are biogenic amines that act as neurotransmitters, neurohormones and neuromodulators in the invertebrate nervous system. Tyramine-β-hydroxylase (TβH) catalyzes the biosynthesis of OA from TA. In this study, cDNA encoding Bombyx mori TβH (BmTβH) was cloned from the head of B. mori. The BmTβH mRNA had 2,204 nucleotide residues, whose open reading frame found to encode 592 amino acid residues. The deduced amino acid sequence was found to have homology to a number of proteins belonging to the insect TβH family. Functional expression of the cloned cDNA was obtained using B. mori baculovirus expression vector system. Western blot analysis exhibited an immunoreactive band with a molecular mass of ~67.4 kDa. Reverse phase high-pressure liquid chromatography (HPLC) has been used to identify the products formed by the incubation of enzyme reaction mixture. BmTβH had the following characteristics; the optimum pH and temperature for the conversion of TA to OA were 7.5 and 25 °C, respectively. As far as the incubation time concerns, the reaction was linear for the first 30 min at 25 °C and pH 7.5. The inhibitory experiments using various concentrations of an inhibitor showed that this method can be applicable for screening of BmTβH inhibitors..
8. Ali, A.M.H., JAE MAN LEE, Yoshida, M., Sakashita, K., Torii, J., takahiro kusakabe, Akinori Hirashima, Expression and characterization of a recombinant Drosophila tyramine-beta-hydroxylase in silkworm infected with recombinant baculovirus, J. Asia-Pac. Entomol., 15, 567-572, 2012.06, Insect nervous system has biogenic amines such octopamine (OA), which is synthesized from tyramine (TA) with the catalysis of tyramine-beta-hydroxylase (TBH). In this study, Drosophila tyramine-beta-hydroxylase (DmTBH) protein with 70 kDa was purified after the recombinant nucleopolyhedrovirus isolated from Bombyx mori (BmNPV) containing TBH gene was injected into hemocoel of fifth instar larvae of d17 Bombyx mori stain. Western blot analysis exhibited an immunoreactive band with a molecular mass of 70 kDa. The products formed by the incubation of enzyme reaction mixture were separated and detected by the reverse phase high-performance liquid chromatography (HPLC). The optimum pH, temperature and incubation time that allow TA to convert into OA were 7.6, 25 °C and 30 min, respectively. The inhibitory experiments using various concentrations of 1-(2-methoxy-5-methylphenyl) imidazole-2(3H)-thione (MMIT) showed that MMIT inhibited DmTH dose dependently and this method can be applicable for screening of DmTH inhibitors..
9. Hirashima A, Regulation of bombykol production by tyramine and octopamine in Bombyx mori., J. Pestic. Sci. , 33 (1), 21-23, 2008.04.
10. Hirashima A, Huang H, Homology modeling, agonist binding site identification, and docking in octopamine receptor of Periplaneta americana., Computational Biology and Chemistry, 32, 185-190, 2008.05.
11. Hirashima A., H. Yamaji, T. Yoshizawa, E. Kuwano and M. Eto , Effect of tyramine and stress on sex-pheromone production in the pre- and post-mating silkworm moth, Bombyx mori. , J. Insect Physiol. , 53 (12), 1242-1249, 2007.12.
12. A. Hirashima, M. Matsushita, H. Ohta, K. Nakazono, E. Kuwano and M. Et, Prevention of progeny formation in Drosophila melanogaster by 1-arylimidazole-2(3H)-thiones, Pestic. Biochem. Physiol., 85(1), 15-20, 2006.05.
13. Hirashima A., M. Kimizu, Y. Shigeta, S. Matsugu, T. Eiraku, E. Kuwano and M. Eto, Pheromone production of female Plodia interpunctella was inhibited by tyraminergic antagonists, Chem. Biodiv., 1(11), 1652-1667, 2004.11.
14. Rauschenbach I. Yu., N. E. Gruntenko, N. S. Chentsova, A. Hirashima, M. Zh. Sukhanova, E. V. Andreenkova, and G. V. Glazko (2001) Interaction of hormones in controlling reproductive function of Drosophila females under stressful conditions is genetically determined. Genetika 37(9), 1243-1350.
15. A. Hirashima, T. Eiraku, E. Kuwano and M. Eto, Comparative Receptor Surface Analysis of Agonists for Tyramine Receptor which Inhibit Sex-Pheromone Production in Plodia interpunctella, Combinatorial Chemistry & High Throughput Screening, 7, 2, 83-91, Vol. 7, No. 2, 83-91, 2004.02.
16. Hirashima A., E. Kuwano and M. Eto, Comparative receptor surface analysis of octopaminergic antagonists for the locust neuronal octopamine receptor, Computional and Biological Chemistry, 10.1016/j.compbiolchem.2003.07.001, 27, 6, 531-540, 27/6, 531-540, 2003.12.
17. Akinori Hirashima ,Tomohiko Eiraku, Eiichi Kuwano, and Morifusa Eto, Three-dimensional molecular-field analyses of agonists for tyramine receptor which inhibit sex-pheromone production in Plodia interpunctella, Internet Electronic Jouranl of Molecular Design, 2(8), 511-526, 2003.05.
18. Akinori Hirashima , Masako Morimoto, Eiichi Kuwano and Morifusa Eto, Three-dimensional molecular-field analyses of octopaminergic agonists for the cockroach neuronal octopamine receptor, Bioorganic and Medicinal Chemistry, 10.1016/S0968-0896(03)00313-4, 11, 17, 3753-3760, 11(17),3753-3760, 2003.07.
19. Akinori Hirashima , Masako Morimoto, Eiichi Kuwano and Morifusa Eto, Octopaminergic agonists for the cockroach neuronal octopamine receptor, Journal of Insect Science, 3, 10, http://insectscience.org/3.10/, 2003.10.
20. Akinori Hirashima , Eiichi Kuwano and Morifusa Eto, Three dimensional receptor surface model of octopaminergic agonists for the locust neuronal octopamine receptor, Internet Electronic Journal of Molecular Design, 2, 274-287, 2003.02.
21. Akinori Hirashima , Yoko Shigeta, Eiichi Kuwano and Morifusa Eto, Inhibitors of calling behavior of Plodia interpunctella, Journal of Insect Science, 3, 4, http://insectscience.org/3.4/, 2003.03.
22. Eto N., S. Kurokui, S. Ikeda, K. Sone, A. Hirashima, Y. Fujimura, K. Tanino, H. Tachibana, M. Yasuda and C-C. Liu, Putative protection mechanism of CTL from killing by their own perforin, Animal Cell Technology: Basic & Applied Aspects, 12, 371-377, 2002.12.
23. Hirashima A., T. Eiraku, Y. Shigeta and E. Kuwano, Three-dimensional pharmacophore hypotheses of octopamine/tyramine agonists which inhibit [1-14C]acetate incorporation in Plodia interpunctella, Bioorganic and Medicinal Chemistry, 10.1016/S0968-0896(02)00320-6, 11, 1, 95-103, 11(1), 95-103, 2003.01.
24. Hirashima A., T. Eiraku, Y. Shigeta, E. Kuwano, E. Taniguchi and M. Eto, Three-dimensional common-feature hypotheses of inhibitors of calling behaviour and in vitro [14C]acetate incorporation by pheromone glands of Plodia interpunctella, Pest Management Science, 10.1002/ps.547, 58, 11, 1118-1125, 58(11), 1118-1125, 2002.11.
25. Hirashima A., M. Morimoto, E. Kuwano, E. Taniguchi and M. Eto, Three-dimensional common-feature hypotheses for octopamine agonist arylethanolamines, Journal of Molecular Graphics and Modeling, 10.1016/S1093-3263(02)00124-9, 21, 2, 81-87, 3(2), 56-68, 2002.02.
26. Three-dimensional common-feature hypotheses of novel inhibitors of calling and in vitro [14C]acetate incorporation by pheromone glands of Plodia interpunctella, Akinori Hirashima, Phytoparasitica, 30(2), 210 (2002).
27. Hirashima A., M. Morimoto, H. Ohta, E. Kuwano, E. Taniguchi and M. Eto, Three-dimensional common-feature hypotheses for octopamine agonist 1-arylimidazolidine-2-thiones, Internet Journal of Molecular Science, 3(2), 56-68, 2002.02.
28. Hirashima A., T. Eiraku, Y. Shigeta, E. Kuwano, E. Taniguchi and M. Eto, Three-dimensional pharmacophore hypotheses of octopamine receptor responsible for the inhibition of sex-pheromone production in Plodia interpunctella, Internet Electronic Journal of Molecular Design, 1(1), 37-51, 2002.01.
29. Hirashima A., M. Morimoto, E. Kuwano, E. Taniguchi and M. Eto, Three-dimensional common-feature hypotheses for octopamine agonist 2-(arylimino)imidazolidines, Bioorganic and Medicinal Chemistry, 10.1016/S0968-0896(01)00247-4, 10, 1, 117-123, 10(1), 117-123, 2002.01.
30. Rauschenbach I. Yu., N. E. Gruntenko, N. S. Chentsova, A. Hirashima, M. Zh. Sukhanova, E. V. Andreenkova, and G. V. Glazko, Regulation of reproductive function in Drosophila females due to hormonal interaction under stress is genetically determined, Russian Journal of Genetics, 10.1023/A:1011961514711, 37, 9, 1041-1047, 37(9), 1041-1047, 2001.09.
31. Hirashima, A., T. Eiraku, Y. Watanabe, E. Kuwano, E. Taniguchi and M. Eto, Identification of Novel Inhibitors of Calling and In Vitro [14C]Acetate Incorporation by Pheromone Glands of Plodia interpunctella, Pesticide Management Science, 10.1002/ps.345, 57, 8, 713-720, 57/8, 713-720, 2001.08.
32. Hirashima A., I. Yu. Rauschenbach and M. Jh. Sukhanova, Ecdysteroids in stress responsive and nonresponsive Drosophila virilis lines under stress conditions, Bioscience, Biotechnology and Biochemistry, 10.1271/bbb.64.2657, 64, 12, 2657-2662, 64/12, 2657-2662, 2000.12.
33. Hirashima A., M. Jh. Sukhanova and I. Yu. Rauschenbach, Biogenic amines in Drosophila virilis under stress conditions, Bioscience, Biotechnology and Biochemistry, 10.1271/bbb.64.2625, 64, 12, 2625-2630, 64/12, 2625-2630, 2000.12.
34. Rauschenbach I. Yu., M. Zh. Sukhanova, A. Hirashima, E. Suetsugu and E. Kuwano, Role of the Ecdysteroid System in the Regulation of Drosophila Reproduction under Environmental Stress, Doklady Biological Sciences, 375(1/6), 641-643, 2000.06.
35. Hirashima A., S. Hirokado, R. Takeya, E. Taniguchi and M. Eto, Metamorphosis of the red flour beetle, Tribolium freemani Hinton(Coleoptera : Tenebrionidae) : Alteration of octopamine modulates activity of juvenile-hormone esterase, ecdysteroid titer, and pupation, Archives of Insect Biochemistry and Physiology, 10.1002/(SICI)1520-6327(1998)37:1<33::AID-ARCH5>3.0.CO;2-W, 37, 1, 33-46, 37/1, 33-46, 1998.01.
36. Hirashima A., C. Pan, J. Tomita, E. Kuwano, E. Taniguchi and M. Eto, Quantitative structure-activity studies of octopaminergic agonists and antagonists against nervous system of Locusta migratoria, Bioorganic and Medicinal Chemistry, 10.1016/S0968-0896(98)00024-8, 6, 7, 903-910, 6/7, 903-910, 1998.06.
37. Hirashima A., K. Shinkai, E. Kuwano, E. Taniguchi and M. Eto, Synthesis and octopaminergic-agonist activity of 3-(substituted phenyl)imidazolidine-2-thiones and related compounds, Bioscience, Biotechnology, and Biochemistry, 10.1271/bbb.62.1179, 62, 6, 1179-1184, 62/6, 1179-1184, 1998.06.
38. Eto M. and A. Hirashima, Stress-induced insect biogenic amines and their analogs as biorequlators, Insects/chemical, Physiological and Environmental Aspects 1997, 55-63, 1998.02.
39. Akinori Hirashima, Three-Dimensional Common-Feature Hypotheses of Novel Inhibitors of Calling and In Vitro [14C]Acetate Incorporation by Pheromone Glands of Plodia interpunctella, Ecologically Sound New Plant Protection Technologies, 22, 2001.09.
40. Sukhanova M. Jh., A. Hirashima., E. Suetsugu, E. Kuwano and I. Yu. Rauschenbach, The system of ecdysteroids is a component of the stress-reaction in Drosophila adults, Drosophila Information Service, 83/7, 4-5, 2000.07.
41. Hirashima A., M. Jh. Sukhanova and I. Yu. Rauschenbach, Genetic control of biogenic - amine systems in Drosophila under normal and Stress conditions, Biochemical Genetics, 38, 5-6, 167-180, 38/5-6,163-176, 2000.05.
42. Hirashima A., K. Shinkai, E. Kuwano, E. Taniguchi and M. Eto, Quantitative structure - activity studies of octopaminergic agonists and antagonists against Locusta migratoria using similarity indexes, SAR and QSAR in Environmental Research, 10.1080/10629360008033228, 11, 1, 45-54, 11/1, 45-54, 2000.01.
43. Hirashima A., E. Kuwano and M. Eto, Docking study of enantiomeric fonofos oxon bound to the active site of Torpedon californica acetylcholinesterase, Bioorganic and Medicinal Chemistry, 10.1016/S0968-0896(99)00315-6, 8, 3, 653-656, 8/3, 653-656, 2000.03.
44. Hirashima A., T. Nagata, C. Pan, E. Kuwano, E. Taniguchi and M. Eto, Three dimensional molecular - field analyses of octopaminergic agonists and antagonists for the locust neuronal octopamine receptor (OAR3), Journal of Graphics and Modelling, 10.1016/S1093-3263(99)00031-5, 17, 3-4, 198-+, 17(3-4), 198-206, 1999.03.
45. Rafaeli A., C. Gileadi and A. Hirashima, Identification of novel synthetic octopamine receptor agonists which inhibit moth sex pheromone production, Pesticide Biochemistry and Physiology, 10.1006/pest.1999.2446, 65, 3, 194-204, 65/3,194-204, 1999.03.
46. Hirashima A., E. Suetsugu, S. Hirokado, E. Kuwano, E. Taniguchi and M. Eto, Effect of octopamine on the activity of juvenile - hormone esterase in the silkworm Bombyx mori and the red flour beetle Tribolium freemani, General and Comparative Endocrinology, 10.1006/gcen.1999.7378, 116, 3, 373-381, 116/3, 373-381, 1999.03.
47. Hirashima A., A. Rafaeli, C. Gileadi and E. Kuwano, Three - dimensional Pharmacophore hypotheses of octopamine receptor responsible for the inhibition of sex-pheromone production in Helicoverpa armigera, Journal of Molecular Graphics and Modelling, 10.1016/S1093-3263(99)00019-4, 17, 1, 43-+, 17/1,43-50, 1999.01.
48. Hirashima A., A. Rafaeli, C. Gileadi and E. Kuwano, Three - dimensional quantitative structure - activity studies of octopaminergic agonists responsible for the inhibition of sex - pheromone production in Helicoverpa armigera, Bioorganic and Medicinal Chemistry, 10.1016/S0968-0896(99)00189-3, 7, 11, 2621-2628, 7/11, 2621-2628, 1999.11.
49. Hirashima A., M. Jh. Sukhanova, E. Kuwano and I. Yu. Rauschenbach, Alteration of biogemic amines in Drosophila virilis under stress, Drosophila Information Service, 82/7, 30-31, 1999.07.
50. Hirashima A., C. Pan, E. Kuwano, E. Taniguchi and M. Eto, Three-dimensional pharmacophore hypotheses for the locust neuronal octopamine receptor (OAR3) : 2. Agonists, Bioorganic and Medicinal Chemistry, 10.1016/S0968-0896(99)00057-7, 7, 7, 1437-1443, 7/7, 1437-1443, 1999.07.
51. Hirashima A., S. Hirokado, H. Ohta, E. Suetsugu, M. Sakaguchi, E. Kuwano, E. Taniguchi and M. Eto, Titres of biogenic amines and ecdysteroids : effect of octopamine on the production of ecdysteroids in the silkworm Bombyx mori, Journal of Insect Physiology, 10.1016/S0022-1910(99)00068-2, 45, 9, 843-851, 45/9, 843-851, 1999.09.
52. Hirashima A., K. Shinkai, C. Pan, E. Kuwano, E. Taniguchi and M. Eto, Quantitative structure-activity studies of octopaminergic ligands against Locusta migratoria and Periplaneta americana, Pesticide Science, 10.1002/(SICI)1096-9063(199902)55:2<119::AID-PS880>3.3.CO;2-N, 55, 2, 119-128, 55/1, 119-128, 1998.01.