Kyushu University Academic Staff Educational and Research Activities Database
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Tomoki   Soh Last modified date:2019.05.20

Assistant Professor / Animal and Marine Bioresource Science, Graduate School of Bioresouce and Bioenvironmental Science
Department of Bioresource Sciences
Faculty of Agriculture


Graduate School
Undergraduate School


Homepage
http://www.agr.kyushu-u.ac.jp/lab/chiku1/lrp-top.html
Home Page of Laboratory of Animal reproductive Physiology .
Academic Degree
Ph. D. (Agriculture)
Country of degree conferring institution (Overseas)
No
Field of Specialization
Animal reproduction
Total Priod of education and research career in the foreign country
00years00months
Outline Activities
Reseach: Studying on implantation mechanism of mouse, and on donor cells for clone embryos of small wild cats.
Education: Laboratory Exercise in Animal Reproduction, Graduation Thesis Research, and lecture of Reproductive biology, Animal reproductive physiology, Animal & Marine Bioresource Sciences.
Research
Research Interests
  • Fundamental study on conservation of small wild cats.
    keyword : small wild cat, conservation
    2018.07~2021.03.
  • Study on the implantation mechanism of rat
    keyword : implantation, uterus, embryo, rat
    2015.04Title: Production of interspecific germ line chimera between quail and chicken. Key words: germ line chimera, quail, chicken We are challenging the hatch of chicken chick from quail egg laid by the chimera quail hen..
Academic Activities
Papers
1. Gakushi Kito, Hiroaki Tanaka, Tomoki   Soh, Nobuhiko Yamauchi, Masa-aki Hattori, The putative promoters of germ cell-specific genes and Nanog are hypomethylated in chicken sperm., Journal of Reproduction and Development, 60, 3, 224-229, 2014.06, Germ cell-specific genes such as Ddx4, Dnd1, and Dazl play critical roles in the proliferation and survival of germ cells. However, the methylation state of the promoter in mature germ cells is still unknown. Here, we investigated the methylation levels of these genes and the pluripotency marker gene Nanog in chicken sperm as compared with the Alb gene in the liver. CpG islands and/or promoter motifs such as TATA box, GC box and CAAT box were found within the putative promoter regions that we identified. By using the bisulfite reaction, CpG sites in the putative promoters were converted, and they were analyzed by sequencing. The putative promoters of Ddx4, Dnd1, Dazl and Nanog showed very low methylation levels in sperm, but they were highly methylated in the liver. Conversely, the Alb gene promoter was highly methylated in sperm and hypomethylated in the liver. However, no transcripts of Ddx4, Dnd1, Dazl and Nanog were detected in sperm or the liver. Also, no transcripts of Dnmt1 and Dnmt3a were detected in sperm. Our present results may indicate that these germ cell-specific genes and the pluripotency marker gene are ready to express any time after fertilization. Our findings showing that low methylation and selective DNA methylation of specific genes are present in chicken sperm contribute to our understanding of fertilization and embryogenesis of birds..
2. 木藤学志、荒牧伸弥、田中浩嗣、宗 知紀、山内伸彦、服部眞彰, Temporal and Spatial Differential Expression of Chicken Germline-specific Proteins cDAZL, CDH and CVH During Gametogenesis , Journal of Reproduction and Development, 56, 3, 341-346, 2010.06, [URL].
3. 荒牧伸弥、久保田海雄、宗 知紀、山内伸彦、服部眞彰, Chicken dead end homologue protein is a nucleoprotein of germ cells including primordial germ cells. , Journal of Reproduction and Development, 55, 2, 214-218, 2009.04.
4. Sinya Aramaki, Fuminori Sato, Takako Kato, Tomoki Soh, Yukio Kato, Masa-aki Hattori, Molecular cloning and expression of dead end homologue in chicken primordial germ cells, Cell and Tissue Research, 330:45-52, 2007.07.
5. 宗 知紀・井上良枝・席 咏梅・加藤幸雄・服部眞彰, Production of interspecific germline chimera between quail and chcken utilizing the cells from central disk of blastoderm and germinal crescent region, J. Fac. Agr. Kyushu Univ., 49, 1, 69-75, Vol. 49, No.1, pp69-75, 2004.01.
6. 宗 知紀・井上良枝・席 咏梅・加藤幸雄・服部眞彰, Utilization of central disk of blastoderm and germinal crescent region for production of interspecific germline chimera between chicken and quail., Asian J. Androl., 4, 2, 83-86, Vol. 4: pp83-86, 2002.06.
Presentations
1. Thein Zaw, 木藤学志, 田中宏明, 宮川愛美, 宗 知紀, 山内 伸彦, 服部 眞彰, Effect of neudesin on the proliferation of chicken PGCs and their characterization, 日本畜産学会, 2014.03.
2. Gakushi Kito, Hiroaki Tanaka, Tomoki   Soh, Nobuhiko Yamauchi, Masa-aki Hattori, Differential regulation of germ cell specific gene expression
between germ cells and somatic cells, International Joint Symposium Between Japan and Korea, 2012.11.
3. Satoshi Nyu, Gakushi Kito, Shinya Aramaki, Tomoki   Soh, Nobuhiko Yamauchi, Masa-aki Hattori, Expression and Function of NENF, Neuron-Derived Neurotrophic Factor, in Chicken Primordial Germ Cells, 米国生殖生物学会(SSR), 2011.07.
4. Intracellular localization and expression of a germline-specific protein cDAZL during chicken gametogenesis.
5. Molecular cloning and expression of dead end homologue in chicken germ cells..
6. Production of Interspecific Germline Chimera between Quail and Chicken Utilizing the Cells from Central Disk of Blastoderm and Germinal Crescent Region.
Soh T, Inoue Y,. Xi YM, Kato Y, Hattori M
West Japan Society of Animal Science, 2003, Naha.
Membership in Academic Society
  • Japanese society of animal reproduction
  • Japan Poultry Science Association
  • Warm regional society animal science, Japan
  • Japanese Avian Endocrinology
  • Japanese Society of Animal Science
Educational
Educational Activities
Underguraduated: Laboratory Exercise in Animal Reproduction, Science English, Graduation Thesis Research

Graduated: Laboratory Exercise in Animal Reproduction, Thesis Research, and lectures of Reproductive biology, Animal reproductive physiology and Animal & Marine Bioresource Sciences.