Kyushu University Academic Staff Educational and Research Activities Database
List of Papers
HIDESHI YASUI Last modified date:2019.07.05

Professor / Agricultural Bioresource Sciences / Department of Bioresource Sciences / Faculty of Agriculture

1. Yamagata, Y., K. T. Win, Y. Miyazaki, C. Ogata, H. Yasui, A. Yoshimura, Development of introgression lines of AA genome Oryza species, O. glaberrima, O. rufipogon, and O. nivara, in the genetic background of O. sativa L. cv. Taichung 65. , Breeding Science, 2019.03.
2. Horgan, F. G., C. C. Bernal, Q. Vu, M. Liberty, P. Almazan, A. F. Ramal, H. Yasui, and D. Fujita,, Virulence adaptation in a rice leafhopper: Exposure to ineffective genes compromises pyramided resistance., Crop Protection , 113:, 40-47, 2018.05.
3. Thu, T. T. P., H. Yasui, and T. Yamakawa, Allocation of macronutrients in roots, sheaths, and leaves determines salt tolerance in rice, American J. of Plant Sci. , 10.1186/s12284-017-0158-1, 9, (5), 2018.03.
4. Norimitsu Hamaoka, Hideshi Yasui, Yoshiyuki Yamagata, Yoko Inoue, Naruto Furuya, Takuya Araki, Ueno Osamu, Atsushi Yoshimura, A hairy-leaf gene, BLANKET LEAF, of wild Oryza nivara increases photosynthetic water use efficiency in rice, Rice, 10.1186/s12284-017-0158-1, 10, 1, 2017.12, Background: High water use efficiency is essential to water-saving cropping. Morphological traits that affect photosynthetic water use efficiency are not well known. We examined whether leaf hairiness improves photosynthetic water use efficiency in rice. Results: A chromosome segment introgression line (IL-hairy) of wild Oryza nivara (Acc. IRGC105715) with the genetic background of Oryza sativa cultivar ‘IR24’ had high leaf pubescence (hair). The leaf hairs developed along small vascular bundles. Linkage analysis in BC5F2 and F3 populations showed that the trait was governed by a single gene, designated BLANKET LEAF (BKL), on chromosome 6. IL-hairy plants had a warmer leaf surface in sunlight, probably due to increased boundary layer resistance. They had a lower transpiration rate under moderate and high light intensities, resulting in higher photosynthetic water use efficiency. Conclusion: Introgression of BKL on chromosome 6 from O. nivara improved photosynthetic water use efficiency in the genetic background of IR24..
5. Takeshi Kuroha, Keisuke Nagai, Usuke Kurokawa, Yoshiaki Nagamura, Miyako Kusano, Hideshi Yasui, Motoyuki Ashikari, Atsushi Fukushima, eQTLS regulating transcript variations associated with rapid internode elongation in deepwater rice, Frontiers in Plant Science, 10.3389/fpls.2017.01753, 8, 2017.10, To avoid low oxygen, oxygen deficiency or oxygen deprivation, deepwater rice cultivated in flood planes can develop elongated internodes in response to submergence. Knowledge of the gene regulatory networks underlying rapid internode elongation is important for an understanding of the evolution and adaptation of major crops in response to flooding. To elucidate the genetic and molecular basis controlling their deepwater response we used microarrays and performed expression quantitative trait loci (eQTL) and phenotypic QTL (phQTL) analyses of internode samples of 85 recombinant inbred line (RIL) populations of non-deepwater (Taichung 65)- and deepwater rice (Bhadua). After evaluating the phenotypic response of the RILs exposed to submergence, confirming the genotypes of the populations, and generating 188 genetic markers, we identified 10,047 significant eQTLs comprised of 2,902 cis-eQTLs and 7,145 trans-eQTLs and three significant eQTL hotspots on chromosomes 1, 4, and 12 that affect the expression of many genes. The hotspots on chromosomes 1 and 4 located at different position from phQTLs detected in this study and other previous studies. We then regarded the eQTL hotspots as key regulatory points to infer causal regulatory networks of deepwater response including rapid internode elongation. Our results suggest that the downstream regulation of the eQTL hotspots on chromosomes 1 and 4 is independent, and that the target genes are partially regulated by SNORKEL1 and SNORKEL2 genes (SK1/2), key ethylene response factors. Subsequent bioinformatic analyses, including gene ontology-based annotation and functional enrichment analysis and promoter enrichment analysis, contribute to enhance our understanding of SK1/2-dependent and independent pathways. One remarkable observation is that the functional categories related to photosynthesis and light signaling are significantly over-represented in the candidate target genes of SK1/2. The combined results of these investigations together with genetical genomics approaches using structured populations with a deepwater response are also discussed in the context of current molecular models concerning the rapid internode elongation in deepwater rice. This study provides new insights into the underlying genetic architecture of gene expression regulating the response to flooding in deepwater rice and will be an important community resource for analyses on the genetic basis of deepwater responses..
6. Thieu Thi Phong Thu, Hideshi Yasui, Takeo Yamakawa, Effects of salt stress on plant growth characteristics and mineral content in diverse rice genotypes, Soil Science and Plant Nutrition, 10.1080/00380768.2017.1323672, 63, 3, 264-273, 2017.03, When rice is grown under moderate salinity (6 dS m−1), yields are reduced by up to 50%. The development of salt-tolerant varieties is a key strategy for increasing yields. We conducted an experiment using a hydroponic system with ion components similar to seawater to determine useful parameters for assessing salt tolerance. Two-week-old seedlings were grown for 7 days on Yoshida hydroponic solution. The treatment group then additionally received an artificial seawater solution (electrical conductivity, 12 dS m−1). After a 2-week period of salt stress, standard evaluation scores (SES) of visual salt injuries were assessed. The K, Na, Mg, and Ca contents were then determined in the roots, sheaths, and leaves of each plant. Following the SES results, we divided the 37 genotypes into four groups: salt-tolerant groups (STGs), moderately salt-tolerant groups, salt-sensitive groups (HSSGs), and highly salt-sensitive groups (HSSGs). In the control, STGs had the highest sheath K content (30.1 mg g−1 dried weight [DW]), whereas HSSGs had the lowest (21.4 mg g−1 DW). Sheath K was also highly and negatively correlated with SES. This suggests that sheath K may be useful for identifying salt-tolerant varieties under non-saline conditions. Plant growth was significantly affected under salt stress, but STGs had the smallest decrease in sheath DW. SES was significantly correlated with sheath and leaf Na, sheath K and Mg, and sheath and leaf Na/K and Na/Mg ratios. The results suggested that sheath K, Na/K, and Na/Mg may be useful indicators for genetic analyses of salt-tolerant varieties under salt-stress conditions. The salt-tolerant cultivars, KCR20, KCR124, and KCR136, are possible candidates for such studies because they had high sheath K content (31.19, 31.21, 29.44 mg g−1 DW, respectively) under non-saline conditions and low SES (3.3, 3.6, 3.9, respectively), and low sheath Na/K (0.64, 0.52, 0.92, respectively) and Na/Mg ratios (2.96, 2.27, 3.03, respectively) under salt-stress conditions..
7. Win, K. T., Y. Yamagata, K. Doi, K. Uyama, Y. Nagai, Y. Toda, T. Tani, M. Ashikari, H. Yasui, and A. Yoshimura, A single base change explains the independent origin of and selection for the nonshattering gene in African rice domestication., New Phytologist, 10.1111/nph.14290, 213, 4, 1925-1935, 2017.03.
8. Mai, T. V., A. Yoshimura and H. Yasui, Characterization of resistance to the green rice leafhopper (Nephotettix cincticeps Uhler) in a core collection of landraces in rice (Oryza sativa L.), American Journal of Plant Sci., 10.4236/ajps.2017.82018, 8, 2, 2017.01.
9. Giao Ngoc Nguyen, Yoshiyuki Yamagata, Yuko Shigematsu, Miyako Watanabe, Yuta Miyazaki, Kazuyuki Doi, Kosuke Tashiro, Satoru Kuhara, Hiroyuki Kanamori, Jianzhong Wu, Takashi Matsumoto, Hideshi Yasui, Atsushi Yoshimura, Duplication and loss of function of genes encoding RNA polymerase III subunit C4 causes hybrid incompatibility in rice, G3: Genes, Genomes, Genetics, 10.1534/g3.117.043943, 7, 8, 2565-2575, 2017.01, Reproductive barriers are commonly observed in both animals and plants, in which they maintain species integrity and contribute to speciation. This report shows that a combination of loss-offunction alleles at two duplicated loci, DUPLICATED GAMETOPHYTIC STERILITY 1 (DGS1) on chromosome 4 and DGS2 on chromosome 7, causes pollen sterility in hybrid progeny derived from an interspecific cross between cultivated rice, Oryza sativa, and an Asian annual wild rice, O. nivara. Male gametes carrying the DGS1 allele from O. nivara (DGS1-nivaras) and the DGS2 allele from O. sativa (DGS2-T65s) were sterile, but female gametes carrying the same genotype were fertile. We isolated the causal gene, which encodes a protein homologous to DNA-dependent RNA polymerase (RNAP) III subunit C4 (RPC4). RPC4 facilitates the transcription of 5S rRNAs and tRNAs. The loss-of-function alleles at DGS1-nivaras and DGS2-T65s were caused by weak or nonexpression of RPC4 and an absence of RPC4, respectively. Phylogenetic analysis demonstrated that gene duplication of RPC4 at DGS1 and DGS2 was a recent event that occurred after divergence of the ancestral population of Oryza from other Poaceae or during diversification of AA-genome species..
10. Kanako Bessho-Uehara, Diane R. Wang, Tomoyuki Furuta, Anzu Minami, Keisuke Nagai, Rico Gamuyao, Kenji Asano, Rosalyn B. Angeles-Shim, Yoshihiro Shimizu, Madoka Ayano, Norio Komeda, Kazuyuki Doi, Kotaro Miura, Yosuke Toda, Toshinori Kinoshita, Satohiro Okuda, Tetsuya Higashiyama, Mika Nomoto, Yasuomi Tada, Hidefumi Shinohara, Yoshikatsu Matsubayashi, Anthony Greenberg, Jianzhong Wu, Hideshi Yasui, Atsushi Yoshimurah, Hitoshi Mori, Susan R. McCouch, Motoyuki Ashikari, Loss of function at RAE2, a previously unidentified EPFL, is required for awnlessness in cultivated Asian rice, Proceedings of the National Academy of Sciences of the United States of America, 10.1073/pnas.1604849113, 113, 32, 8969-8974, 2016.08, Domestication of crops based on artificial selection has contributed numerous beneficial traits for agriculture. Wild characteristics such as red pericarp and seed shattering were lost in both Asian (Oryza sativa) and African (Oryza glaberrima) cultivated rice species as a result of human selection on common genes. Awnedness, in contrast, is a trait that has been lost in both cultivated species due to selection on different sets of genes. In a previous report, we revealed that at least three loci regulate awn development in rice; however, the molecular mechanism underlying awnlessness remains unknown. Here we isolate and characterize a previously unidentified EPIDERMAL PATTERNING FACTOR-LIKE (EPFL) family member named REGULATOR OF AWN ELONGATION 2 (RAE2) and identify one of its requisite processing enzymes, SUBTILISIN-LIKE PROTEASE 1 (SLP1). The RAE2 precursor is specifically cleaved by SLP1 in the rice spikelet, where the mature RAE2 peptide subsequently induces awn elongation. Analysis of RAE2 sequence diversity identified a highly variable GC-rich region harboring multiple independent mutations underlying protein-length variation that disrupt the function of the RAE2 protein and condition the awnless phenotype in Asian rice. Cultivated African rice, on the other hand, retained the functional RAE2 allele despite its awnless phenotype. Our findings illuminate the molecular function of RAE2 in awn development and shed light on the independent domestication histories of Asian and African cultivated rice..
11. Bessho-Uehara, K., D. R.Wang, T. Furuta, A. Minami, K. Nagai, R. Gamuyao, K. Asanoa, R. B. Angeles-Shima, Y. Shimizua, M. Ayanoa, N. Komeda, K. Doi, K. Miura, Y. Toda, T. Kinoshita, S. Okuda, T. Higashiyama, M. Nomoto, Y. Tada, H. Shinohara, Y. Matsubayashi, A. Greenberg, J. Wu, H. Yasui, A. Yoshimura, H. Mori, S. R. McCouch, and M. Ashikari, Loss of function at RAE2, a previously unidentified EPFL, is required for awnlessness in cultivated Asian rice., Proceedings of the National Academy of Sciences, 113(32), 8969-8974, 2016.07.
12. Kurokawa, Y., T. Noda, Y. Yamagata, R. Angeles-Shim, H. Sunohara, K. Uehara, T. Furuta, K. Nagai, K.K. Jena, H. Yasui, A. Yoshimura, M. Ashikari, and K. Doi, Construction of a versatile SNP array for pyramiding useful genes of rice., Plant Sci., Plant Sci., 242, 131-139, 2016.06.
13. Yusuke Kurokawa, Tomonori Noda, Yoshiyuki Yamagata, Rosalyn Angeles-Shim, Hidehiko Sunohara, Kanako Uehara, Tomoyuki Furuta, Keisuke Nagai, Kshirod Kumar Jena, Hideshi Yasui, Atsushi Yoshimura, Motoyuki Ashikari, Kazuyuki Doi, Construction of a versatile SNP array for pyramiding useful genes of rice, Plant Science, 10.1016/j.plantsci.2015.09.008, 242, 131-139, 2016.01, DNA marker-assisted selection (MAS) has become an indispensable component of breeding. Single nucleotide polymorphisms (SNP) are the most frequent polymorphism in the rice genome. However, SNP markers are not readily employed in MAS because of limitations in genotyping platforms. Here the authors report a Golden Gate SNP array that targets specific genes controlling yield-related traits and biotic stress resistance in rice. As a first step, the SNP genotypes were surveyed in 31 parental varieties using the Affymetrix Rice 44K SNP microarray. The haplotype information for 16 target genes was then converted to the Golden Gate platform with 143-plex markers. Haplotypes for the 14 useful allele are unique and can discriminate among all other varieties. The genotyping consistency between the Affymetrix microarray and the Golden Gate array was 92.8%, and the accuracy of the Golden Gate array was confirmed in 3 F2 segregating populations. The concept of the haplotype-based selection by using the constructed SNP array was proofed..
14. Mai, T. V., D. Fujita, M. Matsumura, A. Yoshimura and H. Yasui, Genetic basis of multiple resistance to the brown planthopper (Nilaparvata lugens Stal) and the green rice leafhopper (Nephotettix cincticeps Uhler) in the rice cultivar ‘ASD7’ (Oryza sativa L. ssp. indica), Breed. Sci., 65, 420-429., 2015.12.
15. Tan Van Mai, Daisuke Fujita, Masaya Matsumura, Atsushi Yoshimura, Hideshi Yasui, Genetic basis of multiple resistance to the brown planthopper (Nilaparvata lugens stål) and the green rice leafhopper (nephotettix cincticeps uhler) in the rice cultivar ‘ASD7’ (oryza sativa L. ssp. indica), Breeding Science, 10.1270/jsbbs.65.420, 65, 5, 420-429, 2015.12, The rice cultivar ASD7 (Oryza sativa L. ssp. indica) is resistant to the brown planthopper (BPH; Nilaparvata lugens Stål) and the green leafhopper (Nephotettix virescens Distant). Here, we analyzed multiple genetic resistance to BPH and the green rice leafhopper (GRH; Nephotettix cincticeps Uhler). Using two independent F2 populations derived from a cross between ASD7 and Taichung 65 (Oryza sativa ssp. japonica), we detected two QTLs (qBPH6 and qBPH12) for resistance to BPH and one QTL (qGRH5) for resistance to GRH. Linkage analysis in BC2F3 populations revealed that qBPH12 controlled resistance to BPH and co-segregated with SSR markers RM28466 and RM7376 in plants homozygous for the ASD7 allele at qBPH6 Plants homozygous for the ASD7 alleles at both QTLs showed a much faster antibiosis response to BPH than plants homozygous at only one of these QTLs. It revealed that epistatic interaction between qBPH6 and qBPH12 is the basis of resistance to BPH in ASD7 In addition, qGRH5 controlled resistance to GRH and co-segregated with SSR markers RM6082 and RM3381. qGRH5 is identical to GRH1 Thus, we clarified the genetic basis of multiple resistance of ASD7 to BPH and GRH..
16. Thanga Suja Srinivasan, Maria Liberty P. Almazan, Carmencita C. Bernal, Daisuke Fujita, Angelee Fame Ramal, Hideshi Yasui, Mohan Kumar Subbarayalu, Finbarr G. Horgan, Current utility of the BPH25 and BPH26 genes and possibilities for further resistance against plant- and leafhoppers from the donor cultivar ADR52, Applied Entomology and Zoology, 10.1007/s13355-015-0364-5, 50, 4, 533-543, 2015.11, This study examines the resistance of recently developed near-isogenic rice lines (NILs) with BPH25 and BPH26 genes and the resistance donor cultivar ADR52 against Philippine populations of the brown planthopper, Nilaparvata lugens (Stål). Monogenic and pyramided lines with BPH25 and BPH26 were largely ineffective against the planthopper in a series of laboratory bioassays. In previous studies, BPH25 and a pyramided BPH25 + 26-NIL had been effective in reducing the fitness of N. lugens collected in Mindanao (Philippines); however, the virulence of the planthopper appears to have developed recently in Mindanao so the NILs are now ineffective. ADR52 showed marginal resistance against N. lugens, was more generally resistant against the white-backed planthopper, Sogatella furcifera (Horváth), and had strong resistance against the green leafhopper, Nephotettix virescens (Distant). Resistance against the latter two species was not derived from either BPH25 or BPH26, indicating that the cultivar possesses other resistance genes. For all three insect species, egg laying was constant on ADR52 as the plants aged; however, resistance against nymphs of all three insects increased as ADR52 aged. ADR52 had generally high tolerance against all three insects, which increased under high nitrogen conditions. The results of this study indicate ADR52 is a potential source of further resistance genes against leafhoppers and planthoppers. However, the results also indicate the need to carefully manage resistance genes to avoid adaptation by target insects as has occurred with BPH25 and BPH26..
17. Takayuki Asano, Yasumori Tamura, Hiroe Yasui, Kouji Satoh, Makoto Hattori, Hideshi Yasui, Shoshi Kikuchi, The rice GRH2 and GRH4 activate various defense responses to the green rice leafhopper and confer strong insect resistance, Plant Biotechnology, 10.5511/plantbiotechnology.15.0615a, 32, 3, 215-224, 2015.10, fonfers enhanced resistance to green rice leafhoppers (GRH), Nephotettix cincticeps Uhler. A pyramided line carrying GRH2 and GRH4 (TGRH29) showed strong resistance to GRH insects compared with a GRH2 near-isogenic line (TGRH11), although GRH4 alone did not confer any resistance to GRH. To explore the effects of GRH2 and GRH4 on GRH resistance, we investigated the transcriptional response of rice plants to GRH infestation using DNA microarray analysis. The expression of a large number of genes encoding pathogenesis-related proteins, lipoxygenases, terpene synthase (TPS) and WRKY transcription factor, was upregulated in response to GRH infestation in TGRH11 and TGRH29 compared with control plants. Quantitative RT-PCR revealed that expression of JAmyb and TPS was more strongly and more rapidly upregulated in TGRH29 compared with TGRH11 after GRH infestation. These results suggest that TGRH29 plants can more rapidly and strongly activate the defense response compared with plants carrying GRH2 alone. Furthermore, sesquiterpenes were emitted from TGRH29 plants in response to attack by GRH. The strong induction of sesquiterpene production in the TGRH29 line was correlated with the transcript levels of TPS genes. Our results suggest that GRH2 and GRH4 activate various defense responses and confer strong GRH insect resistance..
18. Asano, T., Y. Tamura, H. Yasui, K. Satoh, M. Hattori, H. Yasui, S. Kikuchi, The rice GRH2 and GRH4 activate various defense responses to the green rice leafhopper and confer strong insect resistance., Plant Biotechnology, 32, 215-224, 2015.07.
19. Srinivasan, T. S., M.L.P. Almazan, C.C. Bernal, D. Fujita, A. F. Ramal, H. Yasui, M. K. Subbarayalu, F. G. Horgan, Current utility of the BPH25 and BPH26 genes and possibilities for further resistance against plant- and leafhoppers from the donor cultivar ADR52, Appl. Entomol. Zool.,, 50, 533-543, 2015.07.
20. Tomoyuki Furuta, Norio Komeda, Kenji Asano, Kanako Uehara, Rico Gamuyao, Rosalyn B. Angeles-Shim, Keisuke Nagai, Kazuyuki Doi, Diane R. Wang, Hideshi Yasui, Atsushi Yoshimura, Jianzhong Wu, Susan R. McCouch, Motoyuki Ashikari, Convergent loss of awn in two cultivated rice species Oryza sativa and Oryza glaberrima is caused by mutations in different loci, G3: Genes, Genomes, Genetics, 10.1534/g3.115.020834, 5, 11, 2267-2274, 2015, A long awn is one of the distinct morphological features of wild rice species. This organ is thought to aid in seed dispersal and prevent predation by animals. Most cultivated varieties of Oryza sativa and Oryza glaberrima, however, have lost the ability to form long awns. The causal genetic factors responsible for the loss of awn in these two rice species remain largely unknown. Here, we evaluated three sets of chromosome segment substitution lines (CSSLs) in a common O. sativa genetic background (cv. Koshihikari) that harbor genomic fragments from Oryza nivara, Oryza rufipogon, and Oryza glaberrima donors. Phenotypic analyses of these libraries revealed the existence of three genes, Regulator of Awn Elongation 1 (RAE1), RAE2, and RAE3, involved in the loss of long awns in cultivated rice. Donor segments at two of these genes, RAE1 and RAE2, induced long awn formation in the CSSLs whereas an O. sativa segment at RAE3 induced long awn formation in O. glaberrima. These results suggest that the two cultivated rice species, O. sativa and O. glaberrima, have taken independent paths to become awnless..
21. Tamura, Y. M. Hattori, H. Yoshioka, M. Yoshioka, A. Takahashi, J. Wu, N. Sentoku and H. Yasui., Map-based cloning and characterization of a brown planthopper resistance gene BPH26 from Oryza sativa L. ssp. indica cultivar ADR52., Sci. Rep., 10.1038/srep05872, 4, 5872, 2014.07.
22. HIDESHI YASUI, Responses and adaptation by Nephotettix virescens to monogenic and pyramided rice lines with Grh-resistance genes. , Entomologia Experimentalis et Applicata 150: 179–190. , DOI: 10.1111/eea.12149, 150, 179-190, 2014.02.
23. Quynh Vu, Reyuel Quintana, Daisuke Fujita, Carmencita C. Bernal, Hideshi Yasui, Celia D. Medina, Finbarr G. Horgan, Responses and adaptation by Nephotettix virescens to monogenic and pyramided rice lines with Grh-resistance genes, Entomologia Experimentalis et Applicata, 10.1111/eea.12149, 150, 2, 179-190, 2014.02, The green leafhopper, Nephotettix virescens (Distant) (Hemiptera: Cicadellidae), occasionally damages rice in Asia either directly, by feeding on the host phloem, or indirectly by transmitting tungro virus. We assessed the nature of resistance against the leafhopper in monogenic and pyramided near-isogenic rice lines containing the resistance genes Grh2 and Grh4. Only the pyramided line was resistant to leafhopper damage. Leafhopper nymphs and adults had high mortality and low weight gain when feeding on the pyramided line and adults laid few eggs. In contrast, although there was some minor resistance in 45-day-old plants that possessed either Grh2 or Grh4 genes, the monogenic lines were generally as susceptible to the leafhopper as the recurrent parent line Taichung65 (T65). Resistance in the pyramided line was stable as the plant aged and under high nitrogen, and affected each of five Philippine leafhopper populations equally. Furthermore, in a selection study, leafhoppers failed to adapt fully to the pyramided resistant line: nymph and adult survival did improve during the first five generations of selection and attained similar levels as on T65, but egg-laying failed to improve over 10 generations. Our preliminary results suggested that resistance was associated with physiological costs to the plants in some experiments. The results of this study demonstrate the success of pyramiding resistance genes through marker-assisted breeding, to achieve a strong and potentially durable resistance. We discuss the utility of gene pyramiding and the development of near-isogenic lines for leafhopper management..
24. JIRAPONG Jairin, TETSUYA Kobayashi, HIDESHI YASUI, A Simple Sequence Repeat- and Single-Nucleotide Polymorphism-Based Genetic Linkage Map of the Brown Planthopper, Nilaparvata lugens 
 , DNA Research 20(1): 17-30 , doi:10.1093/dnares/dss030, 20, 17-30, 2013.02, In this study, we developed the first genetic linkage map for the major rice insect pest, the brown planthopper (BPH, Nilaparvata lugens). The linkage map was constructed by integrating linkage data from two backcross populations derived from three inbred BPH strains. The consensus map consists of 474 simple sequence repeats, 43 single-nucleotide polymorphisms, and 1 sequence-tagged site, for a total of 518 markers at 472 unique positions in 17 linkage groups. The linkage groups cover 1093.9 cM, with an average distance of 2.3 cM between loci. The average number of marker loci per linkage group was 27.8. The sex-linkage group was identified by exploiting X-linked and Y-specific markers. Our linkage map and the newly developed markers used to create it constitute an essential re- source and a useful framework for future genetic analyses in BPH.
Key words: Nilaparvata lugens; brown planthopper; genetic linkage map; SSR; SNP.
25. HIDESHI YASUI, Mapping and pyramiding of two major genes for resistance to the brown planthopper (Nilaparvata lugens Sta ̊l) in the rice cultivar ADR52, Theor Appl Genet, DOI 10.1007/s00122-011-1723-4, 124, 3, 494-504, 2012.02, The brown planthopper (BPH), Nilaparvata 11 lugens(Sta ̊l),isoneofthemostseriousanddestructive 12 pests of rice, and can be found throughout the rice-growing 13 areas of Asia. To date, more than 24 major BPH-resistance 14 genes have been reported in several Oryza sativa ssp. 15 indica cultivars and wild relatives. Here, we report the 16 genetic basis of the high level of BPH resistance derived 17 from an Indian rice cultivar, ADR52, which was previously 18 identified as resistant to the whitebacked planthopper 19 (Sogatella furcifera [Horva ́th]). An F2 population derived 20 from a cross between ADR52 and a susceptible cultivar, 21 Taichung 65 (T65), was used for quantitative trait locus 22 (QTL) analysis. Antibiosis testing showed that multiple 23 loci controlled the high level of BPH resistance in this F2 24 population. Further linkage analysis using backcross pop- 25 ulations resulted in the identification of BPH-resistance 26 (antibiosis) gene loci from ADR52. BPH25 co-segregated 27 with marker S00310 on the distal end of the short arm of
A1 Communicated by T. Tai.
A2 K. K. M. Myint and D. Fujita contributed equally to this work.
A3 K. K. M. Myint .
26. Daisuke Fujita, Atsushi Yoshimura and Hideshi Yasui, Development of near-isogenic lines and pyramided lines carrying resistance genes to green rice leafhopper (Nephotettix cincticeps Uhler) using the Taichung 65 genetic background in rice (Oryza sativa L.), Breed. Sci., 60, 1, 18-27, 60(1): 18-27, 2010.03.
27. Myint, K. K. M., Matsumura M, Takagi M and Yasui H , Demographic parameters of long-term laboratory strains of the brown planthopper, Nilaparvata lugens Stål, (Hompptera: Delphacidae) on resistance genes, bph20(t) and Bph21(t) in rice. , J. Fac. Agr. Kyushu Univ. , 54(1): 159-164, 2009.03.
28. Myint, K. K. M., H. Yasui, M. Takagi and M. Matsumura , Virulence of long-term laboratory populations of the brown planthopper, Nilaparvata lugens (Stål), and whitebacked planthopper, Sogatella furcifera (Horváth) (Homoptera: Delphacidae), on rice differential varieties. , Appl. Entomol. Zool., 44: 149-153, 44: 149-153, 2009.01.
29. Fujita, D., K. Doi, A. Yoshimura and H. Yasui, Molecular mapping of a novel gene, Grh5, conferring resistance to green rice leafhopper (Nephotettix cincticeps Uhler) in rice, Oryza sativa L., Theor Appl Genet., 113(4):567-73, 2006.08.
30. Yasui, H., Genetics and breeding of resistance to brown planthopper, Nilaparvata lugens Stal., in rice., Bull. Inst. Trop. Agr., Kyushu Univ., 28-1(Special issue): 51-55, 2005.11.
31. Wang, C., H. Yasui, A. Yoshimura, H. Zhaia and J. Wan, Inheritance and QTL mapping of antibiosis to green leafhopper in rice., Crop Sci., 44, 2, 389-393, 44:389-393., 2005.01.
32. Su, C. C., J. Wan, H. Q. Zhai, C. M. Wang, L. H. Sun, H. Yasui and A. Yoshimura, A new locus for resistance to brown planthopper identified in the indica rice variety DV85., Plant Breeding, 10.1111/j.1439-0523.2004.01011.x, 124, 1, 93-95, 124:93-95., 2005.01.
33. Kawano, R., T. Mochizuki, H. Yasui, K. Doi and A. Yoshimura, QTL analysis for floating ability in rice., Rice Genet. Newsl., 20:74-82., 2003.12.
34. Fujita, D., K. DOI, A. Yoshimura and H. Yasui, Mapping of a new resistance gene for green rice leafhopper introgressed from Oryza rufipogon Griff. into cultivated rice, Oryza sativa L., Rice Genet. Newsl., 20:81-87., 2003.12.
35. Yamasaki, M., A. Yoshimura and H. Yasui, QTL mapping of rice ovicidal response to two planthopper species., Rice Genet. Newsl., 20:81-87., 2003.12.
36. Sonoda, T., A. Yoshimura and H. Yasui, Detection of QTLs for antibiosis to brown planthopper., Rice Genet. Newsl., 20:83-85., 2003.12.
37. Yamasaki, M., A. Yoshimura and H. Yasui, Genetic basis of ovicidal response to whitebacked planthopper (Sogatella furucifera Horvath) in rice (Oryza sativa L.)., Molecular Breeding, 10.1023/A:1026018821472, 12, 2, 133-143, 12:133-148., 2003.02.
38. Yamasaki, M., A. Yoshimura and H. Yasui, Genetic Basis of Ovicidal Response to Whitebacked Planthopper (Sogatella furcifera Horv?h) in Rice (Oryza sativa L.), Molecular Breeding, 10.1023/A:1026018821472, 12, 2, 133-143, 12:133-143, 2003.01.
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