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Takeo Yamakawa Last modified date:2018.05.28

Associate Professor / Molecular Biosciences
Department of Bioscience and Biotechnology
Faculty of Agriculture


Graduate School
Undergraduate School
Other Organization


E-Mail
Homepage
http://bbs1.agr.kyushu-u.ac.jp/prweb2/b06/yamak/yamakawa.html
Phone
092-642-2847
Fax
092-642-2848
Academic Degree
Ph. D. (Agriculture)
Field of Specialization
Plant Nutrition
Outline Activities
The legumenous plant, soybean forms the nodules, which fix dinitrogen symbiotically, between soybean root nodule bacteria.
I am researching for the aim of maximum usage of the ability of this nitrogen fixation and the character of soybean. In other words, (1) The environment factor which effects on the symbiotic nitrogenous fixation, especially, carbon dioxide concentration of the rhizosphere and the influence of some micro element. (2) The clalification of the function of Rj-genotype specific nodulation gene (Rj-gsn gene) of Bradyrhizobium strains responsed to soybean hauboring Rj-genotyp. (3) The establishment of the inoculation technology using for the high compatibility with soybean cultivar, that is, the difference in nodulation ability (compatibility) seen between the Bradyrhizobium strains and the soybean cultivars. (4) The research related to the difference of the production ability seen characteristically in the soybean cultivars and the breeding of soybean lines producing the high level seed contents of isoflavonoids.
To the college student, Education about the knowledge about the inorganic nutrition of the plant, the nitrogen metabolism in plant and the present condition about these research. To the graduate student, the lecture about the present condition of the research about the various factors concerned with the food production, the research and education about application method for using effectively the biological nitrogen fixation and lowering the environment load.
As for the knowledge which could get it newly through the study, it is announced officially through the academic meeting and so on, and the opinion abou those results is adopted widely, and it wants to deepen these study more. And, it is working on the development of the bamboo powder manure and the development of application technology as one of the effective use of the bamboo material to avoid the erosion of bamboo wood whom it can think about with one of the causes of the forest destruction while environmental disruption is shouted.
 In recent years, Kumamoto Prefecture Kuma-gun Taragi town is set as a model district for the recycling of garbage. Our research group is working on the composting of food waste and the construction of a collection system of garbage from households. In other words, it is working on recycling of available waste resources.
Research
Research Interests
  • Methane fermentation using aquaculture sludge and unused organic matter in Mekong Delta region
    keyword : Compost, methane fermentation, Mekong Delta, unused organic matter, aquaculture sludge
    2015.01.
  • Applied technology to agriculture uses of some unimproved organic materials
    keyword : compost, methane fermentation, pruning branch, bamboo, rice plant, vegitable plant, nitrogen, phosphate and potassium
    2002.04.
  • Biological nitrogenn ffixation and nitrogen metabolism in soybean
    keyword : symbiosis, Bradyrhizobium, soybean, nitrogen Fixation, nitrogen metabolism, transporting substrate
    1989.04The biological nitrogen fixation and nitrogen metabolism of soybean plant.
  • Preference mechanisms of Rj-gene in soybean for nodulation by Bradyrhizobium japonicum
    keyword : Rj-gene, genotype specific gene, compatibility,Bradyrhizobium japonicum.
    1998.04The preference mechanism of Bradyrhizobium japonicum strains by the soybean harbouring Rj-gene.
  • Functions of Rj-gene genotype specific nodulation gene habored by Bradyrhizobium japonicum strains
    keyword : soybean, Bradyrhizobium japonicum, Rj-gene, nodulation gene
    2000.04The function of Rj-genotype specific nodulation gene (Rj-gsn gene) retained by Bradyrhizobium japonicum strains.
  • Effect of environmental factor and characteristics on the biosynthesis of isoflavones of soybean
    keyword : soybean, isoflavone, temperature, cultivar.
    2002.04The effects of soybean cultivars and envioronmental factors on the production of isoflavonoids.
Current and Past Project
  • It is an object of the present invention the construction of an area energy circulation system that combines regeneration biogas and high efficiency fuel cell.
  • It is intended to produce high crop yields of high quality in Vietnam.
Academic Activities
Books
1. Takeo Yamakawa, Yuichi Saeki, Chapter 5: Inoculation Methods of Bradyrhizobium japonicum onSoybean in South-West Area of Japan, In A Comprehensive Survey of International Soybean Research - Genetics, Physiology, Agronomy and Nitrogen Relationships, Ed by James E. Board , INTECH, Pages: 83-114, 2013.02, [URL], Inoculation with efficient rhizobia at the ordinary dose does not increase appreciably the seed yield of soybean because the occupation ratio of the inoculated rhizobial strains in the nodules is very low due to competition with less efficient indigenous rhizobia (1, 2). In order to increase the seed yield by rhizobial inoculation, the occupation ratio of the inoculated strains must be increased. The increase of the occupation ratio has been examined from various viewpoints such as improvement of inoculation method (3). For the screening of efficient and competitive strains, a large number of useful strains had been isolated from mutagenized and recombinant rhizobia (4, 5, 6).
Furthermore, Williams and Lynch (7) who identified a non-nodulating line of soybean among the progenies from the cross between cvs. Lincoln and Richard, showed that the abnormal nodulation reaction was controlled by a single recessive gene of the host plant, rj1. Thereafter, cv. Hardee was found to nodulate ineffectively with Bradyrhizobium japonicum belonging to the strains 3-24-44 and 122 serogroups (8, 9). It was demonstrated that the ineffective nodulation was controlled by a host dominant gene, Rj2. Furthermore, this cultivar was found to nodulate ineffectively with the R. japonicum strain 33 due to the presence of another Rj-gene, Rj3 (10). Cvs. Hill and Amsoy 71 harbor a gene (Rj4) that was responsible for the ineffective nodulation. These Rj4-cultivars were nodulated ineffectively with B. japonicum strain 61 (11). Soybean plants harboring these Rj-genes (Rj-cultivars) were considered to restrict effective nodulation with appropriate serogroups of strains and to prefer certain types of rhizobia for nodulation. If this assumption holds true, planting of Rj-cultivars could increase the populations of rhizobial strains highly compatible with those cultivars in soils. Therefore, the relationship between the Rj-genotypes of soybean and the preference of the Rj-cultivars for various types of Bradyrhizobium strain was examined (12, 13). These Bradyrhizobium strains were classified into three nodulation types, type A, B, and C, based on the compatibility with Rj-cultivars. Nodulation type A strains nodulated with almost all the cultivars except for the rj1-ones (non-nodulating lines) and were preferred by non-Rj-ones. Type B or type C strains nodulated soybean cultivars other than the Rj2Rj3-ones or Rj4-ones, respectively except for rj1-ones and were preferred by Rj4-ones or Rj2Rj3-ones, respectively.
This chapter deals with the developmental process and experimental trial of inoculation methods using effective Bradyrhizobium strains and various Rj-genotypes to increase the yield of soybean.
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2. Y. Saeki, M. Ikeda, T. Yamakawa and Y. Nagatomo, Basic Protocols for Soil Chemistry and Plant Nutrition Study., HAU-JICA ERCB Project, Hanoi, Vietnam, p.1-84, 2002.04.
Papers
1. Aung Zaw Htwe, Seinn Moh Moh, Kyi Moe and Takeo Yamakawa, Effects of co-inoculation of Bradyrhizobium japonicum SAY3-7 and Streptomycesgriseoflavus P4 on plant growth, nodulation, nitrogen fixation, nutrient uptake, and yield of soybean in a field condition, Soil Science and Plant Nutrition, 64, 2, 222-229, 2018.04.
2. Doan Cong Dien, Takeo Yamakawa, Toshihiro Mochizuki, Aung Zaw Htwe, Dry Weight Accumulation, Root Plasticity, and Stomatal Conductance in Rice (Oryza sativa L.) Varieties under Drought Stress and Re-Watering Conditions, American Journal of Plant Sciences, 8, 3189-3206, DOI: 10.4236/ajps.2017.812215, 2017.04.
3. Doan Cong DIEN, Toshihiro MOCHIZUKI and Takeo YAMAKAWA, Morphology and Dry Matter Accumulation in Rice (Oryza sativa L.) Seedlings Under Drought Conditions, Journal of the Faculty of Agriculture Kyushu University, 62, 2, 309-322, 2017.09.
4. Thieu Thi Phong Thu, Yasui Hideshi, Yamakawa Takeo, Effects of salt stress on plant growth characteristics and mineral contents in diverse rice genotypes, Soil Science and Plant Nutrition, 63, 264-273, DOI: 10.1080/00380768.2017.1323672, 2017.06.
5. Kyi Moe, Kumudra Win Mg, Kyaw Kyaw Win, Takeo Yamakawa, Effects of Combined Application of Inorganic Fertilizer and Organic Manures on Nitrogen Use and Recovery Efficiencies of Hybrid Rice (Palethwe-1), American Journal of Plant Sciences, 8, 1043-1064, 2017.04.
6. Kyi Moe, Kumudra Win Mg, Kyaw Kyaw Win, Takeo Yamakawa, Combined Effect of Organic Manures and Inorganic Fertilizers on the Growth and Yield of Hybrid Rice (Palethwe-1), American Journal of Plant Sciences, 8, 1022-1042, 2017.04.
7. Aung Zaw Htwe, Takeo Yamakawa, Incompatible Nodulation of Bradyrhizobium elkanii Strains BLY3-8 and BLY6-1 with Rj3 Gene-Harboring Soybean Cultivars, American Journal of Plant Sciences, 8, 178-190, 2017.02.
8. Aung Zaw Htwe, Yu Kanesaki, Hirofumi Yoshikawa, Hirohito Tsurumaru, Takeo Yamakawa, Draft genome sequences of Bradyrhizobium elkanii strains BLY3-8 and BLY6-1, which are incompatible with Rj3 genotype soybean cultivars., Genome Announcements, 4, 5, e01169-16, 2016.10.
9. Aung Zaw Htwe, Takeo Yamakawa, Low-density co-inoculation with Bradyrhizobium japonicum SAY3-7 and Streptomyces griseoflavus P4 promotes plant growth and nitrogen fixation in soybean cultivars, American Journal of Plant Sciences, 7, 12, 1652-1661, 2016.08.
10. Papa Saliou SARR, Shigeru ARAKI, Didier Aime BEGOUDE, Martin YEMEFACK, Gabriel Ambroise MANGA, Takeo Yamakawa, Aung Zaw HTWE, Phylogeny and nitrogen fixation potential of Bradyrhizobium species isolated from the legume cover crop Pueraria phaseoloides in Eastern Cameroon., Soil Science and Plant Nutrition, 10.1080/00380768.2015.1086279, 62, 1, 13-19, 2016.02.
11. Aung Zaw Htwe, Takeo Yamakawa, Enhanced plant growth and/or nitrogen fixation by leguminous and non-leguminous crops after single or dual inoculation of Streptomyces griseoflavus P4 with Bradyhizobium strains., African Journal of Microbiology Research, 9, 49, 2337-2344, 2015.12.
12. Aung Zaw Htwe, Takeo Yamakawa, Kyi Moe, Doan Cong Dien, Symbiotic effectiveness of different indigenous Bradyrhizobium strains on selected Rj-genes harboring Myanmar soybean cultivars., African Journal of Microbiology Research, 9, 49, 2345-2353, 2015.12.
13. Aung Zaw Htwe, Yuchi Saeki, Kyi Moe, Tomoyuki Sakata, Determining nodulation regulatory (Rj) genes of Myanmar soybean cultivars and their symbiotic effectiveness with Bradyrhizobium japonicum USDA110, American Journal of Plant Sciences, 6, 2799-2810, 2015.11.
14. Tomoyuki Sakata, Yu Kanesaki, Hirofumi Yoshikawa, Hirohito Tsurumaru, Takeo Yamakawa, Draft genome of Bradyrhizobium japonicum Is-1, which is incompatible with Rj2 genotype soybeans., Genome Announcements, 3, 5, e01219-15, 2015.10.
15. Aung Zaw Htwe, Takeo Yamakawa, Papa Saliou Sarr, Tomoyuki Sakata, Diversity and distribution of soybean-nodulation bradyrhizobia isolated from major soybean-growing regions in Myanma., African Journal of Microbiology Research, 9, 43, 2183-2196, 2015.10.
16. Hirohito Tsurumaru, Syougo Hashimoto, Kouhei Okizaki, Yu Kanesaki, Hirofumi Yoshikawa, Takeo Yamakawa, A putative T3SS effector encoded by the MA20_12780 gene in Bradyrhizobium japonicum Is-34 causes the incompatibility with Rj4 genotype soybeans. , Appl. Environ. Microbiol., 81, 17, 5812-5819, 2015.09.
17. Kyi Moe, Takeo Yamakawa, Thieu Thi Phong Thu, Yoshinori Kajihara, NPK accumulation and use efficiencies of Manawthukha rice affected by pre-transplant basal and split applications of nitrogen., Communications in Soil Science and Plant Analysis, 46, 20, 2534-2552, 2015.09.
18. Papa Saliou Sarr, Shunsei Fujimoto, Takeo Yamakawa, Nodulation, Nitrogen Fixation and Growth of Rhizobia-Inoculated Cowpea (Vigna unguiculata L. Walp) In Relation with External Nitrogen and Light Intensity., International Journal of Plant Biology & Researc, 3, 1, 1025-1036, 2015.01.
19. Hirohito Tsurumaru, Yu Kanesaki, Kouhei Okizaki, Syougo Hashimoto, Hirofumi Yoshikawa, Takeo Yamakawa, Draft genome of Bradyrhizobium japonicum Is-34 that is incompatible with Rj4 genotype soybeans., Genome Announcements, 2, 6, e01316-14, 2014.12.
20. Kyi Moe, Takeo Yamakawa, Thieu Thi Phong Thu, Yoshinori Kajihara, The effects of pre-transplant basal and split applications of nitrogen on the growth and yield of Manawthukha rice., Communications in Soil Science and Plant Analysis, 45, 2833-2851, 2014.11.
21. Thieu Thi Phong THU, Takeo Yamakawa, Kyi MOE, Effect of nitrogen application timing on growth, grain yield and eating quality of the KD18 and TH3-3 rice varieties., J. Fac. Agr. Kyushu Univ., 59, 1, 55-64, 2014.02, [URL].
22. Takeo Yamakawa, Youko FUKUSHIMA, Low inoculum densities of Bradyrhizobium japonicum USDA 110 is effective on production of soybean (Glycine max L. Merr.) cultivar Fukuyutaka., J. Fac. Agr. Kyushu Univ., 59, 1, 45-53, 2014.02, [URL].
23. YUICHI SAEKI, SOKICHI SHIRO, TOSHIYUKI TAJIMA, AKIHIRO YAMAMOTO, REIKO SAMESHIMA-SAITO, TAKASHI SATO, Takeo Yamakawa, Mathematical Ecology Analysis of Geographical Distribution of Soybean-Nodulating Bradyrhizobia in Japan., Microbes Environ, 10.1264/jsme2.ME13079, 28, 4, 470-478, 2013.12, [URL].
24. Khin Myat Soe, Takeo Yamakawa, Shogo Hashimoto, Papa Saliou Sarr, Phylogenetic diversity of indigenous soybean bradyrhizobia from different agro-climatic regions in Myanmar, ScienceAsia, 10.2306/scienceasia1513-1874.2013.39.574, 39, 6, 574-583, 2013.12.
25. Papa Saliou SARR, Macoumba DIOUF, Mariama Dalanda DIALLO, Saliou NDIAYE, Rouguiyatou DIA, Aliou GUISSE, Takeo Yamakawa, Effects of Different Types of Litters and Fertilizer Application on Growth and Productivity of Maize (Zea mays L. var. Across 86 Pool 16) in Senegal., J. Fac. Agr. Kyushu Univ., 58, 2, 259-267, 2013.10, [URL].
26. Khin Myat Soe, Takeo Yamakawa, Low-Density Co-Inoculation of Myanmar Bradyrhizobium yuanmingense MAS34 and Streptomyces griseoflavus P4 to Enhance Symbiosis and Seed Yield in Soybean Varieties., American Journal of Plant Sciences, 4, 9, 1879-1892, 2013.09.
27. Khin Myat Soe, Takeo Yamakawa, Evaluation of effective Myanmar Bradyrhizobium strains isolated from Myanmar soybean and effects of coinoculation with Streptomyces griseoflavus P4 
for nitrogen fixation. , Soil Science and Plant Nutrition, 10.1080/00380768.2012.682044, 59, 4, 361-370, 2013.08, [URL].
28. , [URL].
29. Khin Myat Soe, Ampan Bhromsiri, Dumnern Karladee, Takeo Yamakawa, Effects of Endophytic Actinomycetes and Bradyrhizobium japonicum strains on Growth, Nodulation, Nitrogen Fixation and Seed Weight of Different Soybean Varieties., Japanese Society of Soil Science and Plant Nutrition, 10.1080/00380768.2012.682044, 58, 3, 319-325, 2012.06, [URL].
30. Sokichi Shiro, Akihiro Yamamoto, Yosuke Umehara, Masaki Hayashi, Naoto Yoshida, Aya Nishiwaki, Takeo Yamakawa, Yuichi Saeki, Effect of Rj Genotype and Cultivation Temperature on the Community Structure of Soybean-Nodulating Bradyrhizobia, Appl. Environ. Microbiol., 78, 4, 1243-1250, 2012.02.
31. Takeo YAMAKAWA, Masayuki TANAKA, Masao SAKAI and Papa Saliou SARR, Genetic Grouping of Bradyrhizobium StrainsCompatible with Soybean (Glycine max L. Merr.) Harboring Rj-gene by AFLP-fingerprinting Analysis., J. Fac. Agr. Kyushu Univ., 56, 2, 223-229, 2011.10, [URL].
32. Aung Kyaw Myinta, Takeo Yamakawa, Yoshinori Kajihara, Khin Khin Marlar Myint and Takahide Zenmyo , Application of different organic and mineral fertilizers on the growth, yield and nutrient accumulation of rice in a Japanese ordinary paddy field. , Science World Journal, 5, 2, 47-54, 2010.07.
33. Aung Kyaw Myinta, Takeo Yamakawa, Yoshinori Kajihara, Khin Khin Marlar Myint and Takahide Zenmyo , Nitrogen dynamics in a paddy field fertilized with mineral and organic nitrogen sources. , American-Eurasian J. Agric. & Environ. Sci, 7, 2, 221-231, 2010.03.
34. Hoang Thi Bich THAO and Takeo YAMAKAWA, Phosphate absorption of intact komatsuna plants as influenced by phosphite. , Soil Sci. Plant Nutr, 56, 1, 133-139, 2010.02, [URL].
35. Yuichi Saeki, Hiroko Oguro, Isao Akagi, Takeo Yamakawa and Akihiro Yamamoto, Diversity of internal transcribed spacer regions between 16S-23S rRNA genes among the three copies of soybean-nodulating Sinorhizobum strains. , Soil Sci. Plant Nutr, 55, 5, 627-633, 2009.10, [URL].
36. Takeo Yamakawa, Mayuko Yamano and Motoki Ikeda 2009 Effect of applied position of crushed fibrous-banboo and nitrogen fertilizer on yield and N, P, K accumulation of soybean (Glycine max L. Merr.) cv. Fukuyutaka. Jpn. J. Soil Sci. Plant Nutr., 80(4), 379-386


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37. Hoang Thi Bich THAO・Takeo YAMAKAWA・Katsuhiro SHIBATA, Effect of phosphite-phosphate interaction on growth and quality of hydroponic lettuce (Lactuca sativa L.), J. Plant Nutr. Soil Sci, 172, 3, 385-392, 2009.06.
38. Hoang Thi Bich THAO・Takeo YAMAKAWA, Phosphite (Phosphorous acid): Fungicide, fertilizer or bio-stimulator? , Soil Sci. Plant Nutr, 55, 2, 228-234, 2009.04.
39. Papa SALIOU SARR・Takeo YAMAKAWA・Syunsei FUJIMOTO・Yuichi SAEKI・Hoang Thi Bich THAO・Aung Kyaw MYINT , Phylogenic diversity and symbiotic effectiveness of root-nodulating bacteria associated with cowpea in the South-West area of Japan, Microbes Environ., 24 (2), 105-112 , 2009.03.
40. Sarr P. S.,, Khouma M., Sene M., Guisse A., Badiane A. N. and Yamakawa T., Effect of natural phosphate rock enhanced compost on pearl millet-cowpea cropping systems. J. Fac. Agr. Kyushu Univ., 54 (1), 29-35, J. Fac. Agr. Kyushu Univ., 54, 1, 29-35, 2009.02.
41. Takeo Yamakawa, Mayuko Yamano and Motoki Ikeda 2009 Effect of K and Mulching of Crushed Fibrous-Bamboo on Growth and Yield of Soybean (Glycine max L. Merr.) cv. Fukuyutaka. Jpn. J. Soil Sci. Plant Nutr., 80(1), 7-13


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42. Hoang Thi Bich THAO, Takeo YAMAKAWA, Papa Saliou SARR, and Aung Kyaw MYINT, Effects of phosphite, a reduced form of phosphate, on growth and phosphorus nutrition of spinach (Spinacia oleracea L.) ., Soil Sci. Plant Nutr, 54(5), 738-743, 2008.10.
43. Hoang Thi Bich THAO・Takeo YAMAKAWA, Growth of Celery (Apium graveolens var. dulce) as influenced by Phosphite., J. Fac. Agr. Kyushu Univ., 53 (2), 375-378, 2008.10.
44. Hoang Thi Bich THAO, Takeo YAMAKAWA, Katsuhiro SHIBATA, Papa Saliou SARR, and Aung Kyaw MYINT, Growth response of komatsuna (Brassica rapa var. peruviridis) to root and foliar applications of phosphite fertilizer. , Plant and Soil, 54(2), 197-203, 2008.05.
45. Hirohito Tsurumaru, Takeo Yamakawa, Masayuki Tanaka and Masao Sakai, Tn5 mutants of Bradyrhizobium japonicum Is-1 with altered compatibility with Rj2-soybean cultivars., Soil Sci. Plant Nutr., 54(2), 197-203, 2008.04.
46. Papa Saliou SARR, Mamadou KHOUMA, Modou SENE, Aliou GUISSE, Aminata Niane BADIANE , Takeo YAMAKAWA, Effect of Pearl millet-cowpea cropping systems on nitrogen recovery, nitrogen use efficiency and biological fixation using 15N tracer technique., Soil Sci. Plant Nutr., 54 (1), 142-147, 2008.02.
47. Hirohito Tsurumaru, Takeo Yamakawa, Masayuki Tanaka and Masao Saka, The efficient strategy of plasmid rescue from Tn5 mutants derived from Bradyrhizobium japonicum Is-1, based on whole genome sequence information of strain USDA110., J. Fac. Agr. Kyushu Univ., 53 (1), 27-31, 2008.02.
48. Takeo Yamakawa, Yoshie Nakano and Arisa Yamada Methods of purification and quantitative determination of soybean isoflavones with HPLC. Jpn. J. Soil Sci. Plant Nutr., 78, 2, 171-177


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49. Takeo Yamakawa, Yoshie Nakano, Arisa Yamada, Sayuri Kajihara and Toshihiro Mochizuki Difference by soybean cultivar and harvest year of isoflavone content in soybean seed. Jpn. J. Soil Sci. Plant Nutr., 78, 1, 33-38



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50. Takeo Yamakawa, Yoshie Nakano, Sayuri Kajihara and Toshihiro Mochizuki Effect of filling temperature on the transition of isoflavone content in soybean plant. Jpn. J. Soil Sci. Plant Nutr., 78, 1, 39-44

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51. Yamakawa T., Ikeda T., and Ishizuka J., Effects of CO2 Concentration in Rhizosphere on Nodulation and N2 Fixation of Soybean and Cowpea, Soil Science and Plant Nutrition, 50, 5, 713-720, 50, 5, 713-720, 2004.10.
52. Yamakawa T., Hussain A.K.M.A., and Ishizuka J., Soybean preference for Bradyrhizobium japonicum for nodulation. Occupation of Serogroup USDA110 in nodules of soybean plants harboring various Rj-genes grown in a field., Soil Science and Plant Nutrition, 49, 6, 835-841, 49, 6, 835-841, 2003.12.
53. Manolov, I.G., Ikeda, M. and Yamakawa T., Effect of methods of nitrogen application on nitrogen recovery from 15N-labeled urea applied to paddy rice (Oryze sativa L.)., J. Fac. Agr. Kyushu Univ, 48, 1-2, 1-11, 48, 1-2, 1-11., 2003.10.
54. Yamakawa T. and Ishizuka J., Effect of nodulation with Bradyrhizobium japonicum and Shinorhizobium fredii on xylem sap composition of Peking (Glycine max L. Merr.)., Soil Sci. Plant Nutr., 48, 4, 521-527, 48, 4, 521-527, 2002.08.
55. Variental differnces in the effects of flooding on the nodule growth, nitrogen fixing activity in soybean. Shao-H. Zheng, Osamu Kusuda, Hirosaki Makamoto, Takeo Yamalkawa, Tadahiko Furuya and Masataka Fukuyama, J. Fac. Agr. Kyushu Univ. , 56, 3139-144, 2002.
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Presentations
1. Kyi Moe, Seinn Moh Moh, 山川 武夫, Effect of combined organic and inorganic fertilizer on growth and productivity of Komatsuna (Brassica rapa L. var. wakana komatsuna), 日本土壌肥料学会九州支部秋季例会, 2017.09, Objectives: This study was conducted to investigate the effect of combined organic and inorganic fertilizers on the growth and productivity of Komatsuna (Brassica rapa L. var. wakana komatsuna).
Materials and Methods: The three levels of chemical fertilizers (CF) and different combinations of organic manures and inorganic fertilizers were assigned in the randomized complete block design (RCB) with three replications. In this study, three types of poultry manures (PM); (1) ekono hakkou keifun (PME), (2) hakkou keifun (PMH), (3) keifun (PMK) and three types of cow manures (CM); (1) neobi-ru (CMN), (2) gyufun (CMG), (3) hakkou gyufun taihi (CMH) were used as tested manures. The standard rate of chemical fertilizer (100%CF) is 0.5 g NPK (as (NH4)2SO4) and 0.5 g P2O5 (as KH2PO4) and 0.5 g K2O (as K2HPO4) pot-1. The amount of N applied was calculated from estimated mineralizable N (%) based on total N of each manure according to Nishio (2007). Pots without organic and inorganic fertilizers was used as control (NPK0).
Results: There were significant differences in growth parameters and dry matter (DM) among CF levels as well as different combinations of organic and inorganic fertilizers. As the number of leaf plant-1 and leaf length, the higher values were found in CF+PMK combinations, the maximum one in 100% CF+100% PMK. The CMG also provided the higher growth parameters as next to PMK. The 150% CF produced the lower number of leaf, while it obtained the maximum SPAD values and leaf length. Significantly, the plants of 50% CF showed severe N deficiency symptom showing slow growth. The PMK gave the higher DM combined with CF, especially the highest DM in 50% CF+100% PMK. The 150% CF resulted the lower DM than all CF+PMK combinations but got the higher DM than 100% and 50% CF. In combinations with CF, the CMG also has a good performance showing the higher DM than 150% CF. As a virgin soil, the Futsukaichi soil has low fertility observing the lowest growth of plants in the control.
Conclusion: This study suggested that combined use of organic and inorganic fertilizers was effective for enhancing the growth of Komatsuna as well as reducing the chemical fertilizers dose.
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2. Doan Cong Dien, 山川 武夫, Screening for Cold Tolerance in Rice (Oryza sativa L.) at Germination Stage., 日本土壌肥料学会九州支部秋季例会, 2017.09, 〔Objective〕 This study was conducted in order to verify the effect of cold stress condition to rice at germination stage, and to select some cold tolerant rice varieties for further studies.
〔Materials and Methods〕 In this study, 186 rice varieties were used for a screening from April to May, 2016. Seeds of experimental varieties were germinated in a growth chamber under two conditions: 21 days in 13°C (cold stress) and seven days in 25°C (control). The number of germinated seeds was counted daily until 21 days after sowing in cold stress and 7 days after sowing in control. The germination index (GI %) was calculated by the following formula (Farzin et al., 2013): GI % = (G14 + G21)/ 10 x 100; where G14 and G21 are number of germinated seeds at 14 and 21 days after sowing, respectively; 10 is total seed number per rice variety. Coleoptile and radicle length (mm) of germinated seeds were obtained at 21 days after sowing in cold stress and at 7 days after sowing in control. Reduction percentage in coleoptile and radicle length under cold stress in comparison with control condition were calculated also.
〔Results〕 There was a large variation in germination index between varieties. Germination ability was severely affected by cold stress. Only 14 varieties showed the germination index higher than 50%, in which Tumo-Tumo expressed the highest germination index (95%) compared to other varieties. Low temperature significantly depressed the growing of coleoptile and radicle in rice varieties. The average reduction ratios in coleoptile length and radicle length under cold stress condition compared to control were 97.68% and 96.78%, respectively. Gompa 2 expressed the lowest reduction ratio for coleoptile length (94.07%) while Heichiao-chuili-hsiang-keng showed the lowest reduction ratio for radicle length (81.37%) among experimental varieties. Compared to other varieties, ARC 13829 variety retained the longest coleoptile length (8.40 mm) and radicle length (14.20 mm) under cold stress condition.
〔Conclusion〕 Tumo-Tumo, Gompa 2, Heichiao-chuili-hsiang-keng and ARC 13829 varieties were selected as cold tolerant varieties at germination stage and will be used for further experiment.
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3. Thieu Thi Phong Thu, Yasui Hideshi, 山川 武夫, Rice accessions response differently to salt stress with various mineral contents in roots, sheaths and leaves of seedling., ISSAAS (International Society for Southeast Asian Agricultural Science) International Congress, 2017.10, In order to determine the useful salt stress level for screening salt tolerance in rice at young seedling stage under growth chamber condition, this experiment was conducted to compare the responses of 2 varieties of KD18 (sensitive) and KKX (tolerance) against 4 different salt stress levels in mineral content of leaf sheath. After 3 days germination, germinated seeds were grown for 14 days on Yoshida hydroponic solution. Then, artificial sea water was used for stress treatments which were controlled at 2, 4 and 6 dS m-1 EC during 2 weeks. Seedlings which were continued growing on Yoshida solution as control treatment (Y) until the end of experiment. This experiment was carried out in growth chamber at 25oC temperature and 16 hours light and 8 hours dark regime. After two-week period of salt stress, shoot and root were measured for length before plant was cut into shoot and root. After oven drying, shoots was divided into sheaths and leaves, then sheath were cut into small pieces, which used for mineral content determination. The K, Na, Mg and Ca contents were determined by HNO3 digestion method, and followed by atomic absorption spectrophotometer (Z5300, Hitachi).
The sheath K contents decreased when EC levels increased in both varieties. The significant differences in sheath K content between 2 varieties were not obsevered at Y and 2 dS m-1EC, but obsevered at 4 and 6 dS m-1 EC. Those sheath K contents were higher in salt tolerant variety KKX than sensitive variety KD18. The largest difference between two varietes in sheath K content was obsevered at EC 4 dS m-1. In contrast to sheath K content, the sheath Na contents increased when EC levels increased in both rice varieties. The sheath Na contents of two varieties were not different in control treatment. KKX showed significantly lower sheath Na contents in compare with KD18 at 2, 4, 6 dS m-1 EC treatments. The most significant difference of sheath Na content was seen at 4 dS m-1 EC treatment. Mg contents was also expressed as the same with sheath Na contents, but the significant difference was not observerd at 6 dS m-1 EC treatment. Sheath Ca content between 2 varieties were not different in Y and 2 dS m-1 EC treatments, but significantly different in 4 and 6 dS m-1 EC treatments. Salt tolerant variety KKX had significant lower sheath Ca contents than salt sensitive varietiy KD18. The Na/K ratios and Mg/K ratios also were not different in Y and 2 dS m-1 EC treatments, but significant different in 4 and 6 dS m-1 EC treatments. The most clearly difference of these ratios between 2 varieties were at 4 dS m-1 EC treatment. Salt tolerant variety KKX had significant lower ratios than salt sensitive varietiy KD18.
From the results, the 4 dS m-1 EC level was suggested as a good level of salt stress for screening salt tolerance in rice at young seedling stage under growth chamber condition. This salt stress level shows the clearest differences between 2 varieties of not only the sheath K, Na, Mg and Ca contents but also the sheath Na/K and the sheath Mg/K ratios..
4. Aung Zaw Htwe, Seinn Moh Moh, Kyi Moe, 山川 武夫, Effects of co-inoculation of Bradyrhizobium japonicum SAY3-7 and Streptomyces griseoflavus P4 on plant growth, nodulation, nitrogen fixation, nutrient uptake and seed yield of soybean., 第14日韓合同国際シンポジウム(AFELiSA), 2017.11, Nowadays, co-inoculation of nitrogen fixing bacteria with plant growth promoting bacteria has become more popular than a sole inoculation of rhizobia or plant growth promoting bacteria because co-inoculation increases soybean yield and improves sustainability of agriculture (Hungria et al., 2015). Many researchers have reported that co-inoculation of rhizobia with plant growth promoting bacteria improve nodulation and nitrogen fixation (Li and Alexander, 1988; Vessey and Buss, 2002; Figueiredo et al. 2007). It has been reported that co-inoculation of bradyrhizobia with endophytic bacteria S. griseoflavus P4 showed the beneficial effects on N fixation in various soybean cultivars under the green house, environmentally controlled condition and open field condition (Soe et al., 2012; Soe and Yamakawa, 2013a; 2013b). Therefore, this study was conducted to investigate effects of co-inoculation of B. japonicum SAY3-7 and S. griseoflavus P4 on plant growth, nodulation, nitrogen fixation, nutrients accumulation and seed yield of Yezin-6 soybean cultivar.
The experiment was conducted at Kyushu University Farm, Fukuoka Prefecture, Japan (33°37' N, 130°25' E), from July to November 2016. In this study, randomized completely block design was used with three replications. Treatments were an uninoculated control (Control), a single inoculation with S. griseoflavus P4 (P4), a single inoculation of B. japonicum SAY3-7 (SAY3-7), and a co-inoculation of SAY3-7 and P4 (P4 + SAY3-7). Plant samples were collected from five different growth stages: V6 (six unfolded trifoliate leaves), R2 (Full flowering stage), R3.5 (early pod-fill stage) and R5.5 (early seed-fill stage), and R8 (maturity stage). In this study, nitrogen fixations were measured using the acetylene reduction assay (ARA) and ureide methods. After digestion of the nutrients using the H2SO4-H2O digestion method (Ohyama et al. 1991), total N accumulation in the shoot was measured by the indophenol method (Cataldo et al. 1974); total P was analyzed using the ascorbic acid method (Murphy and Riley 1962); and total K, Ca, and Mg were measured using atomic absorption spectrophotometry (Z-5300, Hitachi, Tokyo, Japan).
This study shows that a significant increase in plant growth, nodulation, nitrogen fixation, N, P, K, Ca, and Mg uptakes, yield related parameters and yield was occurred due to the single inoculation of B. japonicum SAY3-7, indicating that SAY3-7 is an effective nitrogen fixing bacterium and had the ability to compete with native bradyrhizobia. Moreover, the result findings of this study highlight that single inoculation of S. griseoflavus P4 had the ability to improve plant growth, nodulation, nitrogen fixation and seed yield compared with control. These indicate that S. griseoflavus P4 is an effective endophytic actinomycete for plant growth, nodulation and nitrogen fixation. Consequently, co-inoculation of SAY3-7 and P4 significantly improved nodule mass; nodule dry weight; shoot and root biomass; N fixation; N, P, K, Ca, and Mg uptake; and seed yield in “Yezin-6” compared with the control and single inoculation treatments. Therefore, combined use of SAY3-7 and P4 will be helpful for soybean production through enhancing plant growth, nodulation, nitrogen fixation and other major nutrient uptakes.
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5. Kyi Moe, Sein Moh Moh, 山川 武夫, Integrated effect of organic and inorganic fertilizers on NPK uptakes and recovery efficiencies, and productivity of Komatsuna (Brassica rapa L. var. wakana komatsuna)., 第14日韓合同国際シンポジウム(AFELiSA), 2017.11, This study was investigated the effect of integrated organic and inorganic fertilizers on NPK uptakes and recovery efficiencies (NPK-RE), and productivity of Komatsuna (Brassica rapa L. var. wakana komatsuna).
The randomized complete block design (RCB) was laid out with three replications. Three types of poultry manures (PM); ekono hakkou keifun (PME), hakkou keifun (PMH), keifun (PMK) and three types of cow manures (CM); neobi-ru (CMN), gyufun (CMG), hakkou gyufun taihi (CMH) were integrated with various levels of chemical fertilizer (CF). The integrated treatments are 14 treatments; 50%CF, 100%CF, 150%CF, 100%CF+50%PMH, 100%CF+50%PMK, 100%CF+50%PME, 100%CF+50%CMG, 100%CF+50%CMN, 100%CF+50%CMH, 50%CF+100%PMH, 50%CF+100%PMK, 50%CF+100%PME, 50%CF+100%CMG, 50%CF+100%CMN, 50%CF+100%CMH. The standard rate of chemical fertilizer (100%CF) is 0.5 g NPK (as (NH4)2SO4) and 0.5 g P2O5 (as KH2PO4) and 0.5 g K2O (as K2HPO4) pot-1. The amount of N applied was calculated from total N and estimated mineralizable N (%) of each manure according to Nishio (2007). Pot without organic and inorganic fertilizers was used as control (NPK 0).
Significant differences were observed in dry matter (DM), NPK uptakes, and NPK-RE (%) affected by CF levels as well as integration of organic and inorganic fertilizers. With the same rate 150%NPK applied, the N, P, Mg and Ca uptakes of (CF+PMK)s integrations were higher than those of 150% CF as well as (CF+CM)s. However, the 100%CF+50%CMN was maximized the K uptake which was similar to those of CF+PMs. On the low fertility of futsukaichi soil, the 100%CF could not provide enough NPK for Komatsuna, showing lower DM. The 100%CF+50%PMs produced the highest DM than 150%CF and 100%CF+CMs. On applying 150% NPK, the maximum NP-RE (%) was also observed in the 100%CF+50%PMK and K-RE (%) in 50%CF+100%PMK, followed by 150%CF and 100%CF. The sole application of chemical fertilizer resulted low NPK-RE(%) in 50%CF, 100%CF and 150%CF. Obviously, the PMK has a great potential for integration of chemical fertilizers while creating higher DM, NPK uptakes and NPK-RE(%).
This study clarify that integrated use of organic and inorganic fertilizers was effectively enhanced the growth, NPK uptakes and recovery efficiency of Komatsuna as well as created the opportunity for reducing the CF usage.
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6. Seinn Moh Moh, Kyi Moe, Aung Zaw Htwe, Yasumasa Obo, 山川 武夫, Effects of fermented sea weed liquid fertilizers on plant growth characteristics and mineral contents of Komatsuna (Brassica rapa L. var. wakana komatsuna)., 第14日韓合同国際シンポジウム(AFELiSA), 2017.11, Seaweeds or macro algae are aquatic plants belonging to the thallophyta of plant kingdom. Seaweeds are rich in minerals, protein, lipid, carbohydrate, vitamins, bromine, iodine etc. In recent years, seaweed extracts as liquid fertilizers have come in market (Divya. and Reddi, 2017). Seaweeds have recently gained importance as foliar sprays for several crops (Thivy, 1961; Metha et al., 1967; Bokil et al., 1974) because the extract contains growth promoting hormones (IAA and IBA), cytokinins, trace elements, vitamins and amino acids (Challen and Hemingway, 1965). The objective of this study is to observe the effect of basal and foliar application of seaweed fermented liquid fertilizer on the plant growth, dry matter accumulation (DMA) and N, P, K, Na, Ca and Mg contents of Komatsuna (Brassica rapa L. var. wakana komatsuna).
Three kinds of pot experiments were conducted at Kyushu University’s Phytoton (25°C and 75% RH), which contain one liter of vermiculite and different types of liquid fertilizer. Expt. 1 was used 4 types of seaweed fermented liquid fertilizers (SLFs; SLF1 (anaerobic) and SLF2 (aerobic) using non-washed Nori (Pyropia yezoensis) and SLF3 (anaerobic) and SLF4 (aerobic) using washed Nori) and Hoagland solution as control (6 treat.). All liquid fertilizers were adjusted total N and K to 7.5 mM and 3 mM, respectively. Expt. 2 was performed the basal and foliar application of SLFs (10 treat.). The concentration of inorganic N and K of 1/2 strength Hoagland solution and SLFs were adjusted to 5 and 3 mM by addition of NH4NO3, respectively. Expt. 3 was tested only the effects of foliar application of 10, 20, 50 times dilution of SLFs in 5 days interval (13 treat.). The 1/4 strength of Hoagland solution added 2.5 mM of NaNO3 was used as liquid fertilizer. All experiments were arranged by the completely randomized design with 3 replications. Growth parameters and SPAD value were recorded 3 days interval. After 35 days, the plant parts (shoot and root) in all experiments were harvested by cutting at the cotyledon node. For N and P contents in plant parts were analyzed by using salicylic acid-H2SO4-H2O2 digestion method (Ohyama et al. 1991), and N determination by Indophenol method (Cataldo et al., 1974), P determination by ascorbic acid method (Murphy and Riley 1962). K, Na, Ca and Mg contents were analyzed by nitric acid digestion method and Atomic absorption spectrophotometer (Z-5300, Hitachi).
In Expt. 1 and 2, plant growth and DMA of SLFs application were depressed compered with Hoagland solution. Furthermore, the color of all plants leaves applied SLFs become dark color and those SPAD value is higher than the Hoagland solution treatment. The reason was derived from P deficiency in all SLFs treatments, because the P contents in plants treated by SLFs were severely low. In Expt. 2, the application of 1/2 Hoagland solution containing NH4NO3 appeared some toxic symptom in shoot parts. This result was thought to reflect the application of NH4NO3. In Expt. 3, there was no toxic symptom occur, because of low concentration of nutrients and use of NaNO3. There were no significantly differences in nutrient contents among all foliar treatment. However, DMA of LF2 foliar application is better than other SLFs. According to the result of all experiments, the nutrients uptakes and growth of all plants by using SLFs is lower than Hoagland solution treatment. Under this experimental condition, since decomposed microorganisms are not present in the rhizosphere, it was thought that not only the organic matter was not mineralized but could not be absorbed. So, under the effective soil microorganisms in soil condition, the effectiveness of SFLs should be considered.

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7. Doan Cong Dien, 山川 武夫, Screening and selection for cold tolerance in rice (Oryza sativa L.) at seedling stage., 第14日韓合同国際シンポジウム(AFELiSA), 2017.11, Rice (Oryza sativa L.) is one of the most important staple food for half of the world population. Unlike other cereals such as wheat and barley, rice plants are susceptible to cold stress, which has negative impacts on germination, vegetative growth, and final productivity. In northern provinces of Vietnam, rice cultivation in spring season (February to June) is usually affected by low temperature at germination and seedling stages, which results in seed loss and delay in transplanting period. In this study, 175 rice varieties from different ecological regions over the world were screened under natural low temperature at seedling stage. The screening was conducted in a greenhouse of Kyushu University from November to December, 2014 in which air temperature was gradually decreased during the experiment. Cold tolerant ability of rice varieties in this screening was verified based on leaf colour scale (LCS) follow the Standard Evaluation System for Rice (IRRI, 2002): 1: Seedlings dark green; 3: Seedlings light green; 5: Seedlings yellow; 7: Seedling brown; 9: Seedlings dead. The screening found a large genotypic variation in leaf colour scale among experimental varieties (ranged from 1 to 9). There were 30 varieties scored at 1 (highly cold tolerant group - HCTG); 31 varieties scored at > 1 to 3 (moderate cold tolerant group); 21 varieties scored at >3 to 5 (cold tolerant group); 49 varieties scored at >5 to 7 (cold sensitive group); and 44 varieties scored at >7 to 9 (highly cold sensitive group - HCSG).
From the screening results, 53 varieties with different leaf colour scales were selected and used for another experiment at seedling stage. During the first week after sowing, seedlings of all varieties were nurtured at 25oC in a biotron. After that, rice seedlings were treated under 2 different conditions including natural low temperature (control) and warmed condition for 2 weeks. For warming treatment, a warming sheet was set at 25oC and put under the seedling trays. The experiment was carried out from March to April, 2015 in which natural air temperature gradually increased through the treatment time. The experimental results shown that warming treatment were significantly increased shoot length (129%), root length (127%) and shoot dry weight (142%) of rice seedlings compared to control condition. There was no significant correlation between leaf colour scale (in 2014 screening) and the warm/control ratio in parameters. This might be explained by the difference in natural low temperature between two experimental times. However, for shoot dry weight, varieties in HCTG showed the lowest warm/control ratio (1.33) while HCSG retained the highest warm/control ratio (1.54). In comparison between varieties, Domzard (LCS=1) expressed the lowest warm/control ratio (0.98) while Namsagui-19 (LCS=9) shown the highest warm/control ratio (2.40) in shoot dry weight accumulation. These suggested that using cold tolerant varieties and/or warming sheet are promising methods to overcome the negative effects of cold stress in rice at seedling stage. In addition, warming sheet is higher effective to cold sensitive varieties compared to cold tolerant varieties.
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8. Thieu Thi Phong Thu, 山川 武夫, Different salt stress affects on mineral contents in leaf sheaths of salt sensitive variety–KD18 and salt tolerant varitety- KKX., 第14日韓合同国際シンポジウム(AFELiSA), 2017.11, In order to determine the useful salt stress level for screening salt tolerance in rice at young seedling stage under growth chamber condition, this experiment was conducted to compare the responses of 2 varieties of KD18 (sensitive) and KKX (tolerance) against 4 different salt stress levels in mineral content of leaf sheath. After 3 days germination, germinated seeds were grown for 14 days on Yoshida hydroponic solution. Then, artificial sea water was used for stress treatments which were controlled at 2, 4 and 6 dS m-1 EC during 2 weeks. Seedlings which were continued growing on Yoshida solution as control treatment (Y) until the end of experiment. This experiment was carried out in growth chamber at 25oC temperature and 16 hours light and 8 hours dark regime. After two-week period of salt stress, shoot and root were measured for length before plant was cut into shoot and root. After oven drying, shoots was divided into sheaths and leaves, then sheath were cut into small pieces, which used for mineral content determination. The K, Na, Mg and Ca contents were determined by HNO3 digestion method, and followed by atomic absorption spectrophotometer (Z5300, Hitachi).
The sheath K contents decreased when EC levels increased in both varieties. The significant differences in sheath K content between 2 varieties were not obsevered at Y and 2 dS m-1EC, but obsevered at 4 and 6 dS m-1 EC. Those sheath K contents were higher in salt tolerant variety KKX than sensitive variety KD18. The largest difference between two varietes in sheath K content was obsevered at EC 4 dS m-1. In contrast to sheath K content, the sheath Na contents increased when EC levels increased in both rice varieties. The sheath Na contents of two varieties were not different in control treatment. KKX showed significantly lower sheath Na contents in compare with KD18 at 2, 4, 6 dS m-1 EC treatments. The most significant difference of sheath Na content was seen at 4 dS m-1 EC treatment. Mg contents was also expressed as the same with sheath Na contents, but the significant difference was not observerd at 6 dS m-1 EC treatment. Sheath Ca content between 2 varieties were not different in Y and 2 dS m-1 EC treatments, but significantly different in 4 and 6 dS m-1 EC treatments. Salt tolerant variety KKX had significant lower sheath Ca contents than salt sensitive varietiy KD18. The Na/K ratios and Mg/K ratios also were not different in Y and 2 dS m-1 EC treatments, but significant different in 4 and 6 dS m-1 EC treatments. The most clearly difference of these ratios between 2 varieties were at 4 dS m-1 EC treatment. Salt tolerant variety KKX had significant lower ratios than salt sensitive varietiy KD18.
From the results, the 4 dS m-1 EC level was suggested as a good level of salt stress for screening salt tolerance in rice at young seedling stage under growth chamber condition. This salt stress level shows the clearest differences between 2 varieties of not only the sheath K, Na, Mg and Ca contents but also the sheath Na/K and the sheath Mg/K ratios..
9. Thieu Thi Phong Thu, 山川 武夫, Effects of salt stress on plant growth characteristics and mineral content of diverse rice genotypes., 第13日韓合同国際シンポジウム(AFELiSA), 2016.11.
10. Doan Cong Dien, 山川 武夫, Effects of Drought to Morphological, Physiological and Biochemical Characteristics of Rice (Oryza sativa L.) at Vegetative Stage., 第13日韓合同国際シンポジウム(AFELiSA), 2016.11.
11. Aung Zaw Htwe, Hirohito Tsurumaru, Yuchi Saeki, Yu Kanesaki, Hirofumi Yoshikawa, 山川 武夫, Characterization of soybean cultivars and Bradyrhizobium strains from Myanmar., 第13日韓合同国際シンポジウム(AFELiSA), 2016.11.
12. Khem Borin, 平井 康丸, 山川 武夫, 森 裕樹, Eiji Inoue, 岡安 崇史, 光岡 宗司, Nitrogen balance in a paddy field cultivated with whole crop rice using cow manure and chemical fertilizer, システム農学会2016年度春季大会, 2016.05.
13. Aung Zaw Htwe, 山川 武夫, Low density co-inoculation of Brayrhizobium japonicum SAY3-7 and Streptomyces griseoflavus P4 promotes plant growth and nitrogen fixation in various soybean varieties, 日本土壌肥料学会全国大会, 2016.09.
14. Takeo Yamakawa, 山田 直隆, 西 幸夫, 岡本啓湖, Decontamination of radioactive cesium by stevia fermented product in Fukushima broccoli cultivation field, The 13th International Conference on Trace Elements, 2015.07.
15. Aung Zaw Htwe, 山川 武夫, Papa Saliou Sarr, Tomoyuki Sakata, Phylogenic diversity and nodulation types of indigenous bradyrhizobia isolated from five major soybean growing regions in Myanmar, 日本土壌肥料学会九州支部春期例会, 2015.04.
16. Khem Borin, 平井 康丸, 山川 武夫, 森 裕樹, Eiji Inoue, 岡安 崇史, 光岡 宗司, KEffects of manure application on growth and yield of rice for whole crop silage, 日本土壌肥料学会全国大会, 2014.09.
17. Khem Borin, 平井 康丸, 山川 武夫, 森 裕樹, Eiji Inoue, 岡安 崇史, 光岡 宗司, Nitrogen balance in the production of rice for whole crop silage, 第68回農業食料工学会九州支部例会, 2014.09.
18. Khin Myat Soe, Takeo Yamakawa, Coinoculation of B. yuanmingense MAS34 and S. griseoflavus P4 to enhance nodulation, N2 fixation and yield of soybean varieties., 18th International Congress on Nitrogen Fixation (18th ICNF), 2013.10, A field experiment was conducted to assess the coinoculation of B. yuanmingense MAS34 and S. griseoflavus P4 to enhance nodulation, N2 fixation and yield of soybean varieties. All the dry matter production, N2 fixation and seed yield were significantly influenced by the effect of inoculated strains alone (P<0.01). The variety effect was also significant (P<0.05) on nodule dry weight at V6 stage, percentage of N derived from atmosphere N2 at R3.5 stage and seed yield at maturity stage. Variety and strains interaction was detected on seed yield at maturity stage (P<0.05). The single inoculation of P4 did not show significant effects on dry matter production, N2 fixation and seed yield of all soybeans compared with uninoculated control. The dry matter production, relative ureide index, percentage of N derived from atmosphere N2 and seed yield were significantly (P<0.01) enhanced mostly by single inoculation of MAS34 and secondly by dual inoculation of P4+MAS34 in Yezin-3 and dual inoculation of P4+MAS34 in Yezin-6. .
19. KYI MOE, 山川 武夫, THIEU THI PHONG THU, Growth and yield parameters of rice variety Manawthukha affected by different application methods of nitrogen and split applications, 日本土壌肥料学会九州支部春期例会, 2013.04.
20. KYI MOE, 山川 武夫, THIEU THI PHONG THU, Effect of different application methods of nitrogen and split applications on NPK accumulations, nitrogen use efficiency of rice variety Manawthukha, 日本土壌肥料学会九州支部春期例会, 2013.04.
21. THIEU THI PHONG THU, 山川 武夫, KYI MOE, The growth parameters and seed quality of inbred and hybrid rice respond to different nitrogen application timings, 日本土壌肥料学会九州支部春期例会, 2013.04.
22. Khin Myat Soe, Takeo Yamakawa, PHYLOGENETIC DIVERSITY OF INDIGENOUS BRADYRHIZOBIA OF SOYBEAN FROM DIFFERENT AGRO-CLIMATIC REGIONS IN MYANMAR, The 2nd Asian Conference on Plant-Microbe Symbiosis and Nitrogen Fixation, 2012.10, Soybean (Glycine max L. Merr.) is the most important grain legume crop in the world and important cash crop in Myanmar. Biological nitrogen fixation is the agronomic importance in reducing the need for chemical nitrogen fertilizer for agriculturally-important crops. This study was aimed to characterize the indigenous bradyrhizobia of soybean from three different agro-climatic regions in Myanmar. In the present study, sequence analysis of the bacterial 16S-23S rRNA internal transcribed spacer (ITS) region was performed to characterize genetically 43 indigenous soybean rhizobia from three different geographic regions (Shan State: Upper Myanmar, Mandalay Region: Middle Myanmar and Yangon Region: Lower Myanmar) of Myanmar. The sequence analysis clustered all isolates in the genus Bradyrhizobium and the collected strains were conspecific with B. japonicum, B. elkanii, B. yuanmingense and Bradyrhizobium sp.. B. yuanmingense was isolated from Shan State and Yangon Region where it attained the frequency of 17% and 12%, respectively. B. japonicum was predominant at Shan State and Mandalay Region with more than 33% of the isolates. B. elkanii was mainly recorded the highest frequency of 88% to Yangon Region, 67% to Mandalay Region and 35% to Shan State. However, B. sp., MAS15 was isolated only from Shan State where it belonged to 4% of isolates. Highest genetic diversity (Shannon’s diversity index) of Bradyrhizobium strains was observed in Shan State soybean growing area. This study revealed that B. elkanii distributed throughout the soybean cultivation areas and recorded as the dominant strains in Myanmar. This is the first on the finding of B. yuanmingense from nodules of soybean and geographical distribution of indigenous bradyrhizobia of soybean from the different regions in Myanmar..
23. Khin Myat Soe, Takeo Yamakawa, EVALUATION OF NODULATION AFFINITY AMONG RJ-GENOTYPE SOYBEANS USING THE BRADYRHIZOBIAL ISOLATED FROM DIFFERENT REGIONS IN MYANMAR, The 2nd Asian Conference on Plant-Microbe Symbiosis and Nitrogen Fixation, 2012.10, Soybean (Glycine max L. Merr.) cultivars form nodules on roots by infection with Bradyrhizobium strains. However, soybean cultivars harbor nodulation conditioning genes, Rj2, Rj3 and Rj4 that ineffective nodules with some strains of Bradyrhizobium. The Bradyrhizobium strains were classified into three types based on their compatibility with Rj-genotype. Type A strains formed effective nodules with all Rj-genotype cultivars and were preferred by the non-Rj-genotype cultivars for nodulation. Type B strains that were incompatible with the Rj2Rj3-genotype were preferred by Rj4-cultivars, whereas type C strains that were incompatible with the Rj4-genotype were preferred by Rj2Rj3-cultivars. In this study, the nodulation type of indigenous bradyrhizobia of soybean on Rj-genotype cultivars was investigated using 43 bradyrhizobia isolated from three different agro-climatic regions (Shan State: Upper Myanmar, Mandalay Region: Middle Myanmar and Yangon Region: Lower Myanmar) in Myanmar. The phylogenetic trees of three different growing areas in Myanmar were constructed based on sequence analysis of the bacterial 16S-23S rRNA internal transcribed spacer (ITS) region. There were examined two nodulation types, type A and B in the Shan State where the nodulation type B was dominant. The Mandalay Region was prominently occupied by the nodulation type A, while in the Yangon Region, the isolated strains were classified as the nodulation type A, B and C. The present study revealed that there were two Rj-genotypes as Rj4- and non-Rj-genotypes among the nine soybean cultivars collected from Myanmar. These findings indicated that the compatibility between Rj4-genotypes soybeans and nodulation type B strains in Shan State and Yangon Region, but in Mandalay region, the compatibility between non-Rj-genotype soybeans and nodulation type A strains was clearly observed..
24. Khin Myat Soe, 山川 武夫, Effects of endophytic actinomycetes (Streptomyces griseoflavus, P4) on different crops and coinoculation with selected Bradyrhizobium strains, 日本土壌肥料学会九州支部秋期例会, 2012.10.
25. Khin Myat Soe, 山川 武夫, Effects of endophytic actinomycetes (Streptomyces griseoflavus, P4) on different crops and coinoculation with selected Bradyrhizobium strains, 日本土壌肥料学会九州支部秋期例会, 2012.10.
26. Papa Saliou Sarr, Shunsei Fujimoto, Takeo Yamakawa, Effect of external nitrogen concentration and light intensity on nodulation, nitrogen
fixation and growth of cowpea (Vigna unguiculata L. Walp.), XV International Congress on Molecular Plant-Microbe Interactions, 2012.07, Cowpea is a legume crop able to fix atmospheric nitrogen with soil rhizobia. The objective of this study was to investigate the concentration of external nitrogen that suppresses nodulation, and the effect of light intensity on the symbiosis and cowpea growth. In a hydroponic culture experiment, nitrogen was supplied at 0, 1, 2.5, 5, 7.5, 10 mM and cowpea was inoculated with Bradyrhizobium yuanmingense strains TSC7, DTC8, and TTC9. The nodulation was strongly inhibited over 7.5 mM N treatment, and only a slight difference was observed between the three strains. However, the application of small amounts (2.5 mM) of nitrogen positively affects the nodulation phenotype (nodule number, nitrogen fixation) at late stage (42 DAT). To assess the effect of Photon flux density on the nodulation phenotype of cowpea associated with nitrogen-fixing rhizobia, 0, 25, 50 and 75 % light intensities were set up. Defoliation was noteworthy in 25 % light intensity at 10 weeks after seedling, and dry weights of pods and seeds were very high compared to the others. No difference was observed in the nodulation phenotype between the three rhizobial strains. Hence, the variation on cowpea growth may arise from the differences between red (R) and far-red (FR) light ratios of treatments. The translocation of carbohydrates might have been accelerated in the 25 % intensity. However, nitrogen concentration of pods and seeds in this treatment was not so different with others. Appropriate shading would therefore, greatly improves cowpea yields, although seed protein content may not vary. .
27. Khin Myat Soe, Takeo Yamakawa, Evaluation of effective Bradyrhizobium strains from Myanmar and co-inoculation with endophytic Streptomyces sp, XV International Congress on Molecular Plant-Microbe Interactions, 2012.07, Soybean (Glycine max L.) is one of the important cash crops in Myanmar. Urea is the main source of nitrogen applied to all crops grown in Myanmar but it is very expensive and not readily available. Rhizobial inoculants can be used to substitute the nitrogenous fertilizers in food legume crops. Indigenous forty-eight root nodules bacteria (MAS1 to MAS48, where MAS means Myanmar Agriculture Service) were collected from different agro-climatic regions of Myanmar in order to evaluate their nitrogen fixing ability for soybean production. After purification, forty-three isolates gave pure colonies and were authenticated for nodule formation on host soybeans in sterilized vermiculite pots in Phytotron (25oC). Based on morphological characteristic, they were identified as Bradyrhizobium strains. These forty-three Bradyrhizobium strains were investigated in symbiosis association with Myanmar recommended soybean, Yezin-6. After inoculation, ten strains were pre-screened based on nitrogen fixation potential determined by using acetylene reduction assay method. When selected ten strains were examined in plant growth and nitrogen fixation with two Myanmar soybeans (Yezin-3 and Yezin-6), MAS23 was found being the most effective strain. The symbiotic relationship between six Bradyrhizobium strains (MAS23, MAS33, MAS34, MAS43, MAS48 and USDA110) and selected endophytic Streptomyces sp. strain (P4) were evaluated with four Myanmar soybean varieties (Yezin-3, Yezin-6, Hinthada and Shan Sein) in pot experiment by using sterilized vermiculite pots in Phytotron (25oC). It was found that dual inoculations of P4 were effectively responded in most of the soybean varieties..
28. Effects of green manure (Sesbania rostrata) on the growth and yield of rice plant and nitrogen fertility in pot experiment.
29. Rice growth, yield and nutrients uptake as affected by the application of rice straw, organic manures and chemical fertilizers as nitrogen sources in paddy field.
30. Isolation and identification root nodulating bacteria from three cowpea (Vigna unguiculata L. Walp.) cultivars.
31. Isolation and identification root nodulating bacteria from three cowpea (Vigna unguiculata L. Walp.) cultivars.
32. Effects of phosphite, a reduced form of phosphate, on the growth and phosphorus nutrition of spinach (Spinacia oleracea L.).
33. Isolation and identification of cowpea nodulating bacteria from the root nodules of three cowpea (Vigna unguiculata L. Walp.) cultivars.
34. Differences in rice production and nitrogen availability in paddy soils amended with organic manures as nitrogen sources.
35. Search for Rj2-gsn Gene of Bradyrhizobium japonicum Is-1 by Tn5 Mutation. Yousuke Ohtsuka, Hirohito Turumaru, Masao Sakai and Takeo Yamakawa, Japanease Society of Molecular Plant Microbe Interactions, 2006. 9.
36. Rj-genotype specific nodulation genes for soybean harboring Rj-genes -Cloning of Tn5 insertion flagment in nodulation mutants of B. japonicum strain Is-1 -. Hirohito Turumaru, Takeo Yamakawa, Natuko Kawanami, Yasuharu Ihara, Masao Sakai and Motoki Ikeda, Society of Japanease Soil Science and Plant Nutrition, 2004.09..
37. Cloning of the Rj-genotype specific nodulation genes for soybean harboring Rj-genes. Hirohito Turumaru, Takeo Yamakawa, Natuko Kawanami, Masao Sakai and Motoki Ikeda, Japanease society of Soil Science Plant Nutrition, 2003.08.
Educational
Educational Activities
Education for the graduate school (Master’s course): Advanced Natural Product Chemistry, Advanced Plant physiology/Biochemistry, Project research on Molecular Biosciences, Special studies on Molecular Biosciences I, Special studies on Molecular Biosciences II
Education for the graduate school (Doctor’s course): Research training on Molecular Biosciences, Teaching practice, Presentation skill for academic meeting, Internship, Project research, Advanced topics on Molecular Biosciences, Tutorial on Molecular Biosciences
Education for the college student: Experiments of Analytical Chemistry, Plant physiology/Biochemistry, Scientific English I, Scientific English II
International Development Research Course (Master’s course): Master’s Thesis Research I, Master’s Thesis Research II, Seminar in a Specified Field I, Seminar in a Specified Field II, Seminar in a Specified Field III
International Development Research Course (Doctor’s course): Research training on Molecular Biosciences, Teaching practice, Presentation skill for academic meeting, Internship, Project research, Advanced topics on Molecular Biosciences, Tutorial on Molecular Biosciences


Experimental Manual: Laboratory Methods for Soil Science and Plant Nutrition.IPSA-JICA Project Publication, 2, p.1-34, IPSA, Bangladesh, 1993. N-15 Tracer Techniques Using Emission Spectrometry.IPSA-JICA Project Publication, 3, p.1-16, IPSA, Bangladesh, 1993.(with J. Haider)
Basic protocols for Soil Chemistry and Plant Nutrition study, HAU-JICA Project, Hanoi, Vietnam, 2002

Intensive course (in Miyazaki University): A methodology for the use of radioisotopes (1998, 1999, 2000,2001, 2002)

Intensive course (in Kagoshima University): Topics in Applied Plant Physiology (2008)
Other Educational Activities
  • 2012.05.
  • 2011.09.
  • 2010.05.
  • 2009.05.
  • 2008.05.
  • 2007.05.
  • 2006.05.
  • 2005.05.
  • 2004.05.
  • 2003.10.
  • 2003.09.
Social
Professional and Outreach Activities
 Strategic International Collaborative Research Program
Science and Technology Research Partnership for Sustainable Development.