Kyushu University Academic Staff Educational and Research Activities Database
List of Papers
Takeo Yamakawa Last modified date:2020.02.03

Associate Professor / Molecular Biosciences / Department of Bioscience and Biotechnology / Faculty of Agriculture


Papers
1. Doan Cong Dien, Thieu Thi Phong Thu, Kyi Moe, Takeo Yamakawa, Proline and carbohydrate metabolism in rice varieties (Oryza sativa L.) under various drought and recovery conditions., Plant Physiology Reports, https://doi.org/10.1007/s40502-019-00462-y, 24, 3, 376-387, 2019.10, Drought stress is a main limitation of rice production in many regions worldwide, as most improved rice varieties are not drought tolerant. This study analyzed the
effects of various drought stresses and subsequent recovery on dry weight accumulation and biochemical characteristics of different rice varieties (the drought-tolerant variety DA8, the drought-sensitive variety Malagkit Pirurutong, and the fast-recovering variety Kinandang Patong). The water treatments were irrigated (control), drought stress, and subsequent re-watering. The results showed that under the same drought stress level, DA8 retained higher water content than the other varieties. In addition, water content in sheaths was higher than that in leaves. DA8 obtained a higher dry weight accumulation than the other varieties under both the control and drought conditions. Proline content in all varieties increased significantly after drought stress. In addition, it accumulated more in leaves than in
sheaths. DA8 expressed a higher ability to accumulate proline than Malagkit Pirurutong or Kinandang Patong. Total soluble sugar and starch contents of the three varieties decreased under drought stress compared to control..
2. Doan Cong Dien, Toshihiro Mochizuki and Takeo Yamakawa, Effect of various drought stresses and subsequent recovery on proline, total soluble sugar and starch metabolisms in rice (Oryza sativa L.) varieties., Plant Production Science, https://doi.org/10.1080/1343943X.2019.1647787, 9, 162, 2019.08, Rice (Oryza sativa L.) is one of the most important staple foods in the world, however most improved rice varieties are susceptible to drought stress. A two-year study was conducted to explore the effects of various drought stresses and subsequent recovery on the accumulation and degradation of proline, total soluble sugar and starch in different rice varieties at vegetative stage. The results showed that relative water content in the leaves and sheaths of rice varieties significantly decreased
under drought stresses, but not at the same rate. Under control and drought conditions, the water content in sheaths was higher than that in leaves. Interestingly, under severe drought stress in 2015, the leaf water content was higher than the sheath water content. The water distribution between leaves and sheaths might be a response of plants to protect leaf system fromdevastation by drought. Proline was highly accumulated under drought stress but rapidly decreased after re-watering. The drought tolerant variety DA8 expressed higher ability in accumulation of proline than susceptible varieties. In general, total soluble sugar and starch contents in leaves and sheaths of varieties decreased under drought stress conditions. Total soluble sugar and starch content of DA8 were less affected than other varieties under drought conditions. Our study indicated that metabolisms of total soluble sugar and starch in rice were affected by both environmental conditions and characteristics
of varieties. Proline accumulation ability of varieties can be used as a useful indicator for drought tolerant potential in rice breeding for water-limited environments..
3. Kyi Moe, #Seinn Moh Moh, #Aung Zaw Htwe, Yoshinori Kajihara, Takeo Yamakawa, Effect of integrated organic and inorganic fertilizers on yield and growth parameters of Japonica and Indica rice (Oryza sativa) varieties., Rice Science, http://dx.doi.org/10.1016/j.rsci.2019.08.005, 26, 5, 309-318, 2019.09, We investigated the effects of integrated organic and inorganic fertilizers on the growth and yield of indica rice variety Manawthukha and japonica rice variety Genkitsukushi. In a split-plot design, the two rice varieties were assigned as main plot factors, and the integrated treatments were the subplot factors, including no-N fertilizer (N0), 50% chemical fertilizer (CF) (CF50), 100% CF (CF100), 50% CF + 50% poultry manure (PM) (CF50PM50), 50% CF + 50% cow manure (CM) (CF50CM50), and 50% CF + 50% compost (CP) (CF50CP50). CF100 was equivalent to N at 85 kg/hm2. Manure was applied based on the estimated mineralizable nitrogen (EMN) level, which is dependent on total N (%) of each manure type. Manawthukha rice plants were taller with higher tiller number and dry matter content. However, higher soil-plant analysis development (SPAD) values were measured in Genkitsukushi throughout the crop growth period, resulting in higher seed-setting rate (%) and greater yield. At the same N level, CF50PM50 application in both rice varieties resulted in higher SPAD values, plant height and tiller number than CF100.
CF50PM50 containing total N more than 4% supplied synchronized N for the demands of the rice plants, resulting in maximum dry matter, yield and yield components. CF50CM50 and CF50CP50 treatments containing total N less than 4% resulted in lower yields which were similar to CF100. These results indicated that integrating organic and inorganic fertilizers enhanced growth parameters and yields of Manawthukha and Genkitsukushi, while reducing the dose of chemical fertilizer..
4. @Jeong Sung Jung, @Jin–Woong Cho and Takeo Yamakawa, Effects of Seeding Date and Cultivars on Yield and Growth Characteristics of Sorghum × Sudangrass Hybrid [Sorghum bicolor (L.) Moench] Cultivars in Central Region of South Korea., Journal of the Faculty of Agriculture Kyushu University, 64, 2, 191-198, 2019.09, [URL], Changing temperatures is one of the major threats to the maintenance of plant yield. Shifting the planting date is the best way to cope with climate change. The aim of this experiment was to determine optimum seeding dates for sorghum × sudangrass hybrid (SSH) cultivars that can provide foundational information to develop improved forage cropping system in central reign of South Korea (CRSK). Three SSHs (Jumbo, Sordan 79 and Revolution) were seeded at four planting dates between April 20 and June 20 (April 20, May 10, May 30 and June 20), respectively. The growing period from the planting to the heading stages was shorter for the cultivars planted on May 10 and longest for those planted on April 20, while those seeded on June 20 could be harvested only once due to the short growing period. Plant height, stem diameter, leaf number, and sugar content were high in the cultivars planted on April 20 and May 10. Among the three cultivars, Jumbo was significantly taller with a large stem diameter, while Revolution was the shortest with a small stem diameter. The highest sugar content was found in Revolution and the lowest in Jumbo. During the 2-year average, the total dry matter yield from the cultivars seeding on April 20 was approximately 54% higher than that of June 20. Additionally, it was difficult to apply seeding on April 20 in the double-cropping system with winter forage because of the prolonged growing period. Based on the yield and agronomic characters observed in this study, seeding on May 10 was ideal for developing improved forage cropping system in CRSK..
5. Kyi Moe, #Seinn Moh Moh, #Aung Zaw Htwe,Takeo Yamakawa, Nitrate, nutrient content and growth parameters of komatsuna (Brassica rapa L.) in response to manure application depending on EMN (estimated mineralizable nitrogen)., Journal of Plant Nutrition, doi.org/10.1080/01904167.2019.1643366, 42, 15, 1726-1739, 2019.08, We investigated the effects of poultry manures (PM) and cow manures (CM) using estimated mineralizable nitrogen (EMN) method on nitrate,
nutrient content and growth parameters of komatsuna and compared with chemical fertilizer (CF) in an abandoned soil. We applied manures as EMN
based on their total N content. The 100% of EMN by PM or CM enhanced dry matter (DM) but depressed nitrate content. The PM-Keifun (PMK) with
total N (4.87%) produced greater growth parameters, DM, nutrient content and lower nitrate content but manures with total N (<2%) could not. The solo CF150 did not promote leaf number but increased nitrate and Na content. In conclusion, a higher total N (>4%) concentration of manure led to increase availability and nutrient contents, DM, and depressed nitrate content of komatsuna, comparable with solo CF in an abandoned soil..
6. Doan Cong Dien and Takeo Yamakawa, Phenotypic variation and selection for cold tolerance in rice (Oryza sativa L.) at both germination and seedling stages., Agriculture, 10.3390/agriculture9080162, 9, 162, 2019.07, Owing to its origin in tropical and subtropical areas, rice is susceptible to cold stress. Low temperatures at the germination and seedling stages can result in seed loss, a delayed transplanting period, and lower final yield. In this study, 181 rice varieties from around the world were investigated for cold tolerance at the germination and seedling stages. At the germination stage, the responses of dierent rice varieties were examined based on the germination index, coleoptile length, and radicle length at low (13 C) and control temperatures (25 C). Significant variations in the germination index, coleoptile length, and radicle length were observed among varieties. Low temperature significantly decreased germination ability, and coleoptile and radicle growth in the studied varieties. At the seedling stage, cold tolerance of the rice varieties was evaluated based on the leaf color score under natural low temperature. Similar to the germination stage, at the seedling stage, significant variation in root and shoot growth was observed in the response of rice varieties to low temperature conditions. Based on the results from both the germination and seedling stages, two varieties (Hei-Chiao-Chui-Li-Hsiang and Ta-Mao-Tao) were selected as the best cold-tolerant varieties. Our results also indicate the benefits of warming treatments to protect rice seedlings from low temperature conditions..
7. Aung Zaw Htwe, Kenjirou Taira, Takeo Yamakawa, Searching for Myanmar indigenous Bradyrhizobium type C strains that best identify Rj
4
genotypes in soybean, Soil Science and Plant Nutrition, https://doi.org/10.1080/00380768.2019.1595137, 65, 3, 228-233, 2019.06, Bradyrhizobium species are symbiotic partners of soybean plants. However, some Bradyrhizobium bacteria do not form functional nodules on the roots of Rj
4
genotype soybean cultivars. Our objective was to identify the strains of Bradyrhizobium (i.e., type C strains) that are least competent to form nodules on the roots of this plant genotype. We checked (i) previously isolated type C strains of Myanmar Bradyrhizobium elkanii (MMY6-1, MMY6-2, and MMY6-5), (ii) previously isolated type C strains of Myanmarese Bradyrhizobium spp. (MMY3-5 and MMY3-7), and (iii) strain Is-34 of B. japonicum, for nodule formation when associated with Rj
4
and other Rj genotype soybeans. Strains in groups (i), (ii), and (iii) are known to be incompatible with Rj
4
soybean genotypes. MMY3-5 and MMY3-7 produced functional nodules when associated with Rj
4
and other Rj genotype soybean cultivars, except Hill (Rj
4
) cultivar. The ratios of ineffective nodule numbers/total nodule numbers (I/T ratios) for MMY6-1, MMY6-2, MMY6-5, and Is-34 in association with Rj
4
soybean cultivars were > 0.5, demonstrating incompatibility between these bacterial strains and the Rj
4
genotype. Interestingly, the I/T ratios of MMY6-1 and MMY6-2 were higher than that of Is-34 in almost all Rj
4
soybean cultivars. Thus, the nodule-forming abilities of the B. elkanii strains MMY6-1 and MMY6-2 were strongly suppressed in Rj
4
soybean cultivars; these strains may therefore be useful to identify the Rj
4
genotype in soybean cultivars..
8. Kyi Moe, Aung Zaw Htwe, Thieu Thi Phong Thu, Yoshinori Kajihara, Takeo Yamakawa, Effects on NPK status, growth, dry matter and yield of rice (Oryza sativa) by organic fertilizers applied in field condition., Agriculture, 10.3390/agriculture9050109, 9, 109, 2019.05, The decline in rice yields as a result of excessive chemical fertilizer (CF) inputs is a matter of great concern in rice-growing regions of Asia. In two-year’s field experiments, the nitrogen, phosphorus, and potassium (NPK) status, growth characteristics and yield of rice were examined by application of poultry manure (PM), cow manure (CM) and compost (CP). Organic fertilizers were applied as EMN (estimated mineralizable N) based on their total N content. Six treatments were assigned in a randomized complete block design: (1) no-N fertilizer (N0); (2) 50% CF (CF50), (3) 100% CF (CF100); 50% CF + 50% EMN from (4) PM or (5) CM or (6) CP. Compared with CF100, the CF50PM50 (total N 4%) accumulated higher N, P and K content in leaf, sheath, panicle and seeds, resulting in greater growth and yield. The CF50PM50 increased yield by 8.69% and 9.70%, dry matter by 4.76% and 5.27% over CF100 in both years. The continuous application of CF50CM50 (total N < 4%) and CF50CP50 (total N < 4%) treatments led to similar NPK contents but higher yields than those of CF100 treatment in 2018. In conclusion, the organic fertilizer (total N 4%) with the EMN method enhances
higher N availability in each year. Continuous application of organic fertilizer (total N < 4%) over two years eectively increased N availability in the second year. The 50% organic fertilizer (total N 4%) and 50% CF led to increased NPK availability and rice yields over the 100% CF treatment, reducing CF usage and leading for sustainable agriculture..
9. Aung Zaw Htwe, Seinn Moh Moh, Kyi Moe, Takeo Yamakawa, Biofertilizer production for agronomic application and evaluation of its symbiotic effectiveness in soybeans, Agronomy, 10.3390/agronomy9040162, 9, 4, 2019.03, This study was conducted to evaluate the effects of Bradyrhizobium japonicum SAY3-7, Bradyrhizobium elkanii BLY3-8, and Streptomyces griseoflavus P4 on the symbiotic effectiveness of soybeans before biofertilizer production, to produce biofertilizer containing the studied three strains (SAY3-7, BLY3-8, and P4), to test the effectiveness of the biofertilizer on soybean varieties, and to assess the varietal effects and interaction effects between variety and biofertilizer on plant growth, nodulation, nitrogen fixation, nutrient absorption, and seed yield. Nitrogen fixation was measured using the acetylene reduction assay and ureide methods. Contents of nutrients (N, P, K, Ca, and Mg) were also measured to calculate their uptakes. In this study, synergistic effects of nitrogen fixation were induced by combined inoculation with SAY3-7, BLY3-8 and P4 in all tested soybean varieties. Therefore, we assumed that an effective biofertilizer could be produced using these effective bacteria (SAY3-7, BLY3-8, and P4). After making biofertilizer using these effective bacteria, packages were stored at 30

C. The populations of the bacteria in the biofertilizer were maintained at a density of 1 × 10
8
colony forming units (cfu) g


1
for P4 and 7 × 10
9
cells g


1
for Bradyrhizobium. Diluting biofertilizer by 10


3
proved more effective for nodulation and nitrogen fixation than other dilution treatments. Moreover, this biofertilizer significantly promoted plant growth, nodulation, nitrogen fixation, nutrient uptakes, and seed yield in Yezin-3 and Yezin-6 soybean varieties. Yezin-6 is a more efficient variety than Yezin-3 for improved plant growth, nodulation, nitrogen fixation, nutrient absorption, and seed yield. Taken together, the application of an effective biofertilizer and the use of an efficient soybean variety can play important roles in promoting plant growth, nodulation, nitrogen fixation, and higher seed yield..
10. Aung Zaw Htwe, Seinn Moh Moh, Khin Myat Soe, Kyi Moe, Takeo Yamakawa, Effects of biofertilizer produced from bradyrhizobium and streptomyces griseoflavus on plant growth, nodulation, nitrogen fixation, nutrient uptake, and Seed Yield of Mung Bean, Cowpea, and Soybean, Agronomy, 10.3390/agronomy9020077, 9, 2, 2019.02, The use of biofertilizers is important for sustainable agriculture, and the use of nodule bacteria and endophytic actinomycetes is an attractive way to enhance plant growth and yield. This study tested the effects of a biofertilizer produced from Bradyrhizobium strains and Streptomyces griseoflavus on leguminous, cereal, and vegetable crops. Nitrogen fixation was measured using the acetylene reduction assay. Under N-limited or N-supplemented conditions, the biofertilizer significantly promoted the shoot and root growth of mung bean, cowpea, and soybean compared with the control. Therefore, the biofertilizer used in this study was effective in mung bean, cowpea, and soybean regardless of N application. In this study, significant increments in plant growth, nodulation, nitrogen fixation, nitrogen, phosphorus, and potassium (NPK) uptake, and seed yield were found in mung beans and soybeans. Therefore, Bradyrhizobium japonicum SAY3-7 plus Bradyrhizobium elkanii BLY3-8 and Streptomyces griseoflavus are effective bacteria that can be used together as biofertilizer for the production of economically important leguminous crops, especially soybean and mung bean. The biofertilizer produced from Bradyrhizobium and S. griseoflavus P4 will be useful for both soybean and mung bean production..
11. Masayuki Sugawara, Satoko Takahashi, Yosuke Umehara, Hiroya Iwano, Hirohito Tsurumaru, Haruka Odake, Yuta Suzuki, Hitoshi Kondo, Yuki Konno, Takeo Yamakawa, Shusei Sato, Hisayuki Mitsui, Kiwamu Minamisawa, Variation in bradyrhizobial NopP effector determines symbiotic incompatibility with Rj2-soybeans via effector-triggered immunity, Nature communications, 10.1038/s41467-018-05663-x, 9, 1, 2018.12, Genotype-specific incompatibility in legume–rhizobium symbiosis has been suggested to be controlled by effector-triggered immunity underlying pathogenic host-bacteria interactions. However, the rhizobial determinant interacting with the host resistance protein (e.g., Rj2) and the molecular mechanism of symbiotic incompatibility remain unclear. Using natural mutants of Bradyrhizobium diazoefficiens USDA 122, we identified a type III-secretory protein NopP as the determinant of symbiotic incompatibility with Rj2-soybean. The analysis of nopP mutations and variants in a culture collection reveal that three amino acid residues (R60, R67, and H173) in NopP are required for Rj2-mediated incompatibility. Complementation of rj2-soybean by the Rj2 allele confers the incompatibility induced by USDA 122-type NopP. In response to incompatible strains, Rj2-soybean plants activate defense marker gene PR-2 and suppress infection thread number at 2 days after inoculation. These results suggest that Rj2-soybeans monitor the specific variants of NopP and reject bradyrhizobial infection via effector-triggered immunity mediated by Rj2 protein..
12. Borin Khem, Yasumaru Hirai , Takeo Yamakawa, Yuki Mori, Eiji Inoue, Takashi Okayasu, Muneshi Mitsuoka, NPK balances in wholc crop ricc cultivation under diffcrcnt application rncthods of mantlrc alld chcllnical fcrtilizcr, システム農学, 64, 4, 87-99, 2018.12.
13. Seinn Moh Moh, Kyi Moe, Yasumasa Obo, Shiniti Obo, Aung Zaw Htwe, Takeo Yamakawa, Effects of Fermented Nori (Pyropia yezoensis) Seaweed Liquid Fertilizers on Growth Characteristics, Nutrient Uptake, and Iodine Content of Komatsuna (Brassica rapa L.) Cultivated in Soil., American Journal of Plant Sciences, 10.4236/ajps.2018.911161, 9, 2227-2243, 2018.10, We investigated the effects of fermented nori (Pyropia yezoensis ) liquid fertilizers on plant growth, soil plant analysis development (SPAD) values, and
nutrient uptake of komatsuna (Brassica rapa L. var. wakana komatsuna) plants. The four types of fermented nori seaweed liquid fertilizers (SLFs) evaluated
in this study were prepared by anaerobic fermentation of unwashed nori (SLF1), aerobic fermentation of unwashed nori (SLF2), anaerobic fermentation
of washed nori (SLF3), and aerobic fermentation of washed nori (SLF4). In Experiment 1, the highest plant growth, SPAD, and nutrient uptake
values were obtained from treatment with SLF2 and SLF4. There were no significant differences between the effects of basal and foliar application of
SLFs, except for iodine (I) content; plants treated with SLF1 had the highest I content. In Experiment 2, plant growth and nutrient uptake decreased with
higher concentrations of SLFs. Plants treated with 25% SLF2 + 75% chemical fertilizer (CF) or 25% SLF4 + 75% CF exhibited significantly higher plant
growth and nutrient uptake. The highest I content resulted from treatment with 75% SLF1 + 25% CF or 100% SLF1. Taken together, our results showed
that 25% SLF + 75% CF produced the best plant growth characteristics, nutrient uptake, and I content relative to the controls. Therefore, basal application
of these liquid organic fertilizers can be used to increase productivity, nutrient uptake, and I content and to reduce nitrate-nitrogen content in komatsuna production..
14. Aung Zaw Htwe, Takeo Yamakawa, Selection of type A and type B strains for improving symbiotic effectiveness on non-Rj and Rj4 genotype soybean varieties, Soil Science and Plant Nutrition, 10.1080/00380768.2018.1510285, 64, 5, 566-575, 2018.09, The selection of effective rhizobia for higher efficiency nitrogen fixation is one of the most important steps for inoculant production. Therefore, this experiment was conducted to select the most effective type A and type B strains for specific Rj-gene harboring soybean varieties and to test the symbiotic effectiveness of selected strains on different Rj-gene harboring soybean varieties. Screening experiments using the specific soybean varieties were done with a completely randomized design and three replications in this study. Evaluation of the effective Myanmar Bradyrhizobium strains for plant growth, nodulation and N2 fixation were studied in pot experiments using sterilized vermiculite in the Phytotron (controlled-environmental condition). Then, a pot experiment was conducted using Futsukaichi soil in the screen house (natural environmental condition). The N2 fixation ability of soybean was evaluated by acetylene reduction activity (ARA) and the relative ureide index method. In the first screening experiment, type A and type B strains with higher nitrogen fixation and proper nodulation on their respective soybean cultivars were selected for the next screening. In the second screening, Bradyrhizobium elkanii AHY3-1 (type A), Bradyrhizobium japonicum SAY3-7 (type A), B. elkanii BLY3-8 (type B) and B. japonicum SAY3-10 (type B) isolates, which showed higher nitrogen fixation and nodulation in Yezin-3 (Rj4) and Yezin-6 (non-Rj), were selected for the next experiment. In the third screening experiment, SAY3-7 and BLY3-8, which had higher nitrogen fixing potential and proper nodulation, were selected as effective isolates. These two isolates were compatible with non-Rj and Rj4 soybean varieties for nodulation and nitrogen fixation. Based on the results of the screening experiment, these two strains were tested for their symbiotic efficacy in Futsukaichi soil. This study shows that inoculation treatment of SAY3-7 and BLY3-8 significantly increased plant growth, nodulation, and N2 fixation at the V6, R3.5 and R8 stages in Yezin-3 (Rj4) and/or Yezin-6 (non-Rj), and the seed yield at R8 stage, in Yezin-3 (Rj4) and Yezin-6 (non-Rj) soybean varieties compared with the control treatment. It can be concluded that SAY3-7 and BLY3-8 are suitable for inoculant production because of their higher nitrogen fixation ability, proper nodulation and better productivity of Myanmar soybean cultivars..
15. Aung Zaw Htwe, Seinn Moh Moh, Kyi Moe and Takeo Yamakawa, Effects of co-inoculation of Bradyrhizobium elkanii BLY3-8 and Streptomyces griseoflavus P4 on Rj4 soybean varieties., Soil Science and Plant Nutrition, 10.1080/00380768.2018.1452574, 64, 4, 449-454, 2018.07, Co-inoculation of selected nitrogen-fixing bacteria with plant growth-promoting bacteria is the promising way for the improvement of soybean production through enhancing plant growth, nodulation, and N2 fixation. Therefore, this experiment was conducted to study the effects of co-inoculation of Bradyrhizobium elkanii BLY3-8 with Streptomyces griseoflavus P4 on plant growth, nodulation, N2 fixation, N uptake, and seed yield of Rj4 soybean varieties. Two experiments with completely randomized design and three replicates were done in this study. N2-fixation ability of soybean was evaluated by acetylene reduction activity (ARA) and relative ureide method. In the first experiment, synergetic effect in N2 fixation and nodulation was occurred in co-inoculation treatment (BLY3-8 + P4) in Yezin-3 and Fukuyutaka. Based on these results, co-inoculation effect of BLY3-8 and P4 was assessed on Yezin-3
and Fukuyutaka varieties at three different growth stages, using Futsukaichi soil under natural environmental conditions. This study shows that co-inoculation of BLY3-8 and P4 significantly increased N2 fixation at V6 stage; plant growth, nodulation, N2 fixation, and N uptake at R3.5 stage; and shoot
growth, N uptake, and seed yield at R8 stage, in Rj4 soybean varieties compared with the control. Significant difference in plant growth, nodulation, N2 fixation, N uptake, and yield between co-inoculation and control, not between single inoculation and control, suggests that there is a synergetic effect due to co-inoculation of BLY3-8 and P4..
16. Seinn Moh Moh, Kyi Moe, Yasumasa Obo, Shiniti Obo, Aung Zaw Htwe, Takeo Yamakawa , Effects of Fermented Nori (Pyropia yezoensis) Liquid Fertilizer on Plant Growth Characteristics and Nutrient Content of Komatsuna (Brassica rapa L. var. Wakana Komatsuna) Cultivated in Vermiculite., American Journal of Plant Sciences, 10.4236/ajps.2018.98116, 9, 1601-1617, 2018.07, We conducted plant growth experiments in microbe-free vermiculite to study the effects of four types of fermented seaweed liquid fertilizer (SLF) made from nori (Pyropia yezoensis ) seaweed on the germination, plant growth characteristics, SPAD value, and nutrient content and uptake of komatsuna (Brassica rapa L. var. wakana komatsuna). The four types of fermented nori SLF used in this study were prepared by anaerobic fermentation of unwashed
nori (SLF1), aerobic fermentation of unwashed nori (SLF2), anaerobic fermentation of washed nori (SLF3), and aerobic fermentation of washed nori
(SLF4). Komatsuna seeds treated with 200-, 300-, and 400-fold dilutions of SLFs exhibited improved relative germination ratios (RGRs) at 3 and 4 days
after sowing (DAS). At 4 DAS, the RGRs of seeds treated with 10-, 100-, 200-, 300-, and 400-fold SLF dilutions showed no differential effect. Seeds treated
with undiluted SLFs did not germinate by 4 DAS. SLF1 may promote komatsuna seed germination. The nitrogen (N), calcium, magnesium, sodium (Na),
and iodine (I) contents of plants treated with SLF1 were significantly increased relative to plants treated with the other SLFs. Moreover, the I and Na
contents of plants were significantly increased by foliar spray application of different dilutions of SLF1. However, SLF treatment markedly reduced the
shoot dry weight compared with ½-strength modified Hoagland nutrient (MHN) solution, although the same amounts of N and K were applied. SPAD
values of the plants treated with SLFs were significantly higher than those of plants treated with MHN. Foliar treatment with SLFs had no significant effect
on plant growth, SPAD value, or uptake of nutrients (except Na) relative to the control, but the I content was increased. Plants treated with SLF1 and
SLF2 exhibited the highest Na uptake. Foliar spray treatments with SLF1 resulted in the highest I contents in plants. Based on our results, SLF1 is suitable
for use as a liquid fertilizer to promote germination and increase nutrient content in komatsuna. These results need to be followed up in soil experiments
in the presence of microbes in the rhizosphere..
17. Borin Khem, Hirai Yasumaru, Takeo Yamakawa, Yuki Mori, Eiji Inoue, Takashi OKAYASU, Mitsuoka Muneshi, Effects of different application methods of fertilizer and manure on soil chemical properties and yield in whole crop rice cultivation., Soil Science and Plant Nutrition, 64, 3, 406-414, 2018.06.
18. Aung Zaw Htwe, Seinn Moh Moh, Kyi Moe and Takeo Yamakawa, Effects of co-inoculation of Bradyrhizobium japonicum SAY3-7 and Streptomycesgriseoflavus P4 on plant growth, nodulation, nitrogen fixation, nutrient uptake, and yield of soybean in a field condition, Soil Science and Plant Nutrition, 64, 2, 222-229, 2018.04.
19. Thieu Thi Phong Thu, Yasui Hideshi, Yamakawa Takeo, Allocation of Macronutrients in Roots, Sheaths, and Leaves Determines Salt Tolerance in Rice., American Journal of Plant Sciences, org/10.4236/ajps.2018.95081, 9, 1051-1069, 2018.04, To determine useful parameters for salt tolerance in rice and selection of salt-tolerant varieties, their macronutrient contents in roots, sheaths, and leaves were evaluated under salt stress condition. A hydroponic experiment was conducted to evaluate 29 rice varieties for salt tolerance. The salt stress treatment included an artificial seawater solution (electrical conductivity of 12 dS∙m−1). After a 2-week period of salt stress, standard evaluation scores (SES) of visual injuries for salt stress were assessed. In addition, we measured the contents of N, P, K, Na, Mg, and Ca in roots, sheaths, and leaves. The results showed that differences in macronutrients in the different plant tissues correlated with rice tolerance to the salt stress condition. Under the control treatment, salt-tolerant varieties exhibited low K content in root. Under the salt stress treatment, the salt-tolerant varieties exhibited low SES, high N content in leaves and sheaths, low Na content in leaves and sheaths, low Mg content in leaves and sheaths, and low Ca content in sheaths. The salt-tolerant varieties also exhibited high salt stress treatment/control treatment (ST/CT) ratios for dry matter in sheaths, N content in leaves and sheaths, and K content in sheaths, and low Na/K ratios in leaves and sheaths. Therefore, these parameters might be useful to understand salt tolerance in rice..
20. Doan Cong DIEN, Toshihiro MOCHIZUKI and Takeo YAMAKAWA, Morphology and Dry Matter Accumulation in Rice (Oryza sativa L.) Seedlings Under Drought Conditions, Journal of the Faculty of Agriculture Kyushu University, 62, 2, 309-322, 2017.09.
21. Thieu Thi Phong Thu, Yasui Hideshi, Yamakawa Takeo, Effects of salt stress on plant growth characteristics and mineral contents in diverse rice genotypes, Soil Science and Plant Nutrition, 63, 264-273, DOI: 10.1080/00380768.2017.1323672, 2017.06.
22. Yusuke Shiratori, Takeo Yamakawa, Mio Sakamoto, Hinomi Yoshida, Takuya Kitaoka, Quang Tuyen Tran, Duc Chanh Tin Doan and Mau Chien Dang, Biogas Production from Local Biomass Feedstock in the Mekong Delta and Its Utilization for a Direct Internal Reforming Solid Oxide Fuel Cell, Frontiers in Environmental Science, 5, doi: 10.3389/fenvs.2017.00025, 2017.05.
23. Kyi Moe, Kumudra Win Mg, Kyaw Kyaw Win, Takeo Yamakawa, Combined Effect of Organic Manures and Inorganic Fertilizers on the Growth and Yield of Hybrid Rice (Palethwe-1), American Journal of Plant Sciences, 8, 1022-1042, 2017.04.
24. Kyi Moe, Kumudra Win Mg, Kyaw Kyaw Win, Takeo Yamakawa, Effects of Combined Application of Inorganic Fertilizer and Organic Manures on Nitrogen Use and Recovery Efficiencies of Hybrid Rice (Palethwe-1), American Journal of Plant Sciences, 8, 1043-1064, 2017.04.
25. Doan Cong Dien, Takeo Yamakawa, Toshihiro Mochizuki, Aung Zaw Htwe, Dry Weight Accumulation, Root Plasticity, and Stomatal Conductance in Rice (Oryza sativa L.) Varieties under Drought Stress and Re-Watering Conditions, American Journal of Plant Sciences, 8, 3189-3206, DOI: 10.4236/ajps.2017.812215, 2017.04.
26. Aung Zaw Htwe, Takeo Yamakawa, Incompatible Nodulation of Bradyrhizobium elkanii Strains BLY3-8 and BLY6-1 with Rj3 Gene-Harboring Soybean Cultivars, American Journal of Plant Sciences, 8, 178-190, 2017.02.
27. Aung Zaw Htwe, Yu Kanesaki, Hirofumi Yoshikawa, Hirohito Tsurumaru, Takeo Yamakawa, Draft genome sequences of Bradyrhizobium elkanii strains BLY3-8 and BLY6-1, which are incompatible with Rj3 genotype soybean cultivars., Genome Announcements, 4, 5, e01169-16, 2016.10.
28. Aung Zaw Htwe, Takeo Yamakawa, Low-density co-inoculation with Bradyrhizobium japonicum SAY3-7 and Streptomyces griseoflavus P4 promotes plant growth and nitrogen fixation in soybean cultivars, American Journal of Plant Sciences, 7, 12, 1652-1661, 2016.08.
29. Papa Saliou SARR, Shigeru ARAKI, Didier Aime BEGOUDE, Martin YEMEFACK, Gabriel Ambroise MANGA, Takeo Yamakawa, Aung Zaw HTWE, Phylogeny and nitrogen fixation potential of Bradyrhizobium species isolated from the legume cover crop Pueraria phaseoloides in Eastern Cameroon., Soil Science and Plant Nutrition, 10.1080/00380768.2015.1086279, 62, 1, 13-19, 2016.02.
30. Youjin LIM, Kyosuk LEE, Dongsung LEE, Jin-Woong CHO, Takeo Yamakawa, Dougyoung CHUNG, Contents of Cations and Anions and Characterization of Stem Cell Wall Structures on Stem of Red Pepper Plants Infected by Phytophthora capsici. , J. Fac. Agr. Kyushu Univ., 61, 1, 23-28, 2016.02.
31. Aung Zaw Htwe, Takeo Yamakawa, Kyi Moe, Doan Cong Dien, Symbiotic effectiveness of different indigenous Bradyrhizobium strains on selected Rj-genes harboring Myanmar soybean cultivars., African Journal of Microbiology Research, 9, 49, 2345-2353, 2015.12.
32. Aung Zaw Htwe, Takeo Yamakawa, Enhanced plant growth and/or nitrogen fixation by leguminous and non-leguminous crops after single or dual inoculation of Streptomyces griseoflavus P4 with Bradyhizobium strains., African Journal of Microbiology Research, 9, 49, 2337-2344, 2015.12.
33. Aung Zaw Htwe, Yuchi Saeki, Kyi Moe, Tomoyuki Sakata, Determining nodulation regulatory (Rj) genes of Myanmar soybean cultivars and their symbiotic effectiveness with Bradyrhizobium japonicum USDA110, American Journal of Plant Sciences, 6, 2799-2810, 2015.11.
34. Aung Zaw Htwe, Takeo Yamakawa, Papa Saliou Sarr, Tomoyuki Sakata, Diversity and distribution of soybean-nodulation bradyrhizobia isolated from major soybean-growing regions in Myanma., African Journal of Microbiology Research, 9, 43, 2183-2196, 2015.10.
35. Tomoyuki Sakata, Yu Kanesaki, Hirofumi Yoshikawa, Hirohito Tsurumaru, Takeo Yamakawa, Draft genome of Bradyrhizobium japonicum Is-1, which is incompatible with Rj2 genotype soybeans., Genome Announcements, 3, 5, e01219-15, 2015.10.
36. Kyi Moe, Takeo Yamakawa, Thieu Thi Phong Thu, Yoshinori Kajihara, NPK accumulation and use efficiencies of Manawthukha rice affected by pre-transplant basal and split applications of nitrogen., Communications in Soil Science and Plant Analysis, 46, 20, 2534-2552, 2015.09.
37. Hirohito Tsurumaru, Syougo Hashimoto, Kouhei Okizaki, Yu Kanesaki, Hirofumi Yoshikawa, Takeo Yamakawa, A putative T3SS effector encoded by the MA20_12780 gene in Bradyrhizobium japonicum Is-34 causes the incompatibility with Rj4 genotype soybeans. , Appl. Environ. Microbiol., 81, 17, 5812-5819, 2015.09.
38. Borin Khem, Hirai Yasumaru, Takeo Yamakawa, 森 裕樹, Eiji Inoue, Takashi OKAYASU, Mitsuoka Muneshi, Production of rice for whole crop silage using manure in Itoshima region, Fukuoka prefecture. , J. Fac. Agr. Kyushu Univ., 60, 1, 65-71, 2015.02.
39. Lu-Jing GAN, Xiang-Yu WANG, Dan YANG, ung-Ah SHIN, Ki-Teak LEE, eung- Hee LEE, Takeo Yamakawa, Jin-Woong CHO, Comparison of nutritional composition of premature, mature and de-hulled barley in Korea., J. Fac. Agr. Kyushu Univ., 60, 1, 57-63, 2015.02.
40. Papa Saliou Sarr, Shunsei Fujimoto, Takeo Yamakawa, Nodulation, Nitrogen Fixation and Growth of Rhizobia-Inoculated Cowpea (Vigna unguiculata L. Walp) In Relation with External Nitrogen and Light Intensity., International Journal of Plant Biology & Researc, 3, 1, 1025-1036, 2015.01.
41. Hirohito Tsurumaru, Yu Kanesaki, Kouhei Okizaki, Syougo Hashimoto, Hirofumi Yoshikawa, Takeo Yamakawa, Draft genome of Bradyrhizobium japonicum Is-34 that is incompatible with Rj4 genotype soybeans., Genome Announcements, 2, 6, e01316-14, 2014.12.
42. Kyi Moe, Takeo Yamakawa, Thieu Thi Phong Thu, Yoshinori Kajihara, The effects of pre-transplant basal and split applications of nitrogen on the growth and yield of Manawthukha rice., Communications in Soil Science and Plant Analysis, 45, 2833-2851, 2014.11.
43. Takeo Yamakawa, Youko FUKUSHIMA, Low inoculum densities of Bradyrhizobium japonicum USDA 110 is effective on production of soybean (Glycine max L. Merr.) cultivar Fukuyutaka., J. Fac. Agr. Kyushu Univ., 59, 1, 45-53, 2014.02, [URL].
44. Thieu Thi Phong THU, Takeo Yamakawa, Kyi MOE, Effect of nitrogen application timing on growth, grain yield and eating quality of the KD18 and TH3-3 rice varieties., J. Fac. Agr. Kyushu Univ., 59, 1, 55-64, 2014.02, [URL].
45. Khin Myat Soe, Takeo Yamakawa, Shogo Hashimoto, Papa Saliou Sarr, Phylogenetic diversity of indigenous soybean bradyrhizobia from different agro-climatic regions in Myanmar, ScienceAsia, 10.2306/scienceasia1513-1874.2013.39.574, 39, 6, 574-583, 2013.12.
46. YUICHI SAEKI, SOKICHI SHIRO, TOSHIYUKI TAJIMA, AKIHIRO YAMAMOTO, REIKO SAMESHIMA-SAITO, TAKASHI SATO, Takeo Yamakawa, Mathematical Ecology Analysis of Geographical Distribution of Soybean-Nodulating Bradyrhizobia in Japan., Microbes Environ, 10.1264/jsme2.ME13079, 28, 4, 470-478, 2013.12, [URL].
47. Papa Saliou SARR, Macoumba DIOUF, Mariama Dalanda DIALLO, Saliou NDIAYE, Rouguiyatou DIA, Aliou GUISSE, Takeo Yamakawa, Effects of Different Types of Litters and Fertilizer Application on Growth and Productivity of Maize (Zea mays L. var. Across 86 Pool 16) in Senegal., J. Fac. Agr. Kyushu Univ., 58, 2, 259-267, 2013.10, [URL].
48. Khin Myat Soe, Takeo Yamakawa, Low-Density Co-Inoculation of Myanmar Bradyrhizobium yuanmingense MAS34 and Streptomyces griseoflavus P4 to Enhance Symbiosis and Seed Yield in Soybean Varieties., American Journal of Plant Sciences, 4, 9, 1879-1892, 2013.09.
49. , [URL].
50. Khin Myat Soe, Takeo Yamakawa, Evaluation of effective Myanmar Bradyrhizobium strains isolated from Myanmar soybean and effects of coinoculation with Streptomyces griseoflavus P4 
for nitrogen fixation. , Soil Science and Plant Nutrition, 10.1080/00380768.2012.682044, 59, 4, 361-370, 2013.08, [URL].
51. Sun-Ok CHUNG, Ki-Hyun CHO, Jin-Woong CHO, Ki-Youl JUNG, Takeo Yamakawa, Soil Texture Classification Algorithm Using RGB Characteristics of Soil Images, Journal of the Faculty of Agriculture, Kyushu University, 57, 2, 393-397, 2012.10, [URL].
52. Sun-Ok CHUNG, Jin-Woong CHO, Takeo Yamakawa, Development of a Motorized Digital Cone Penetrometer., Journal of the Faculty of Agriculture, Kyushu University, 57, 2, 399-404, 2012.10, [URL].
53. Khin Myat Soe, Ampan Bhromsiri, Dumnern Karladee, Takeo Yamakawa, Effects of Endophytic Actinomycetes and Bradyrhizobium japonicum strains on Growth, Nodulation, Nitrogen Fixation and Seed Weight of Different Soybean Varieties., Japanese Society of Soil Science and Plant Nutrition, 10.1080/00380768.2012.682044, 58, 3, 319-325, 2012.06, [URL].
54. Sokichi Shiro, Akihiro Yamamoto, Yosuke Umehara, Masaki Hayashi, Naoto Yoshida, Aya Nishiwaki, Takeo Yamakawa, Yuichi Saeki, Effect of Rj Genotype and Cultivation Temperature on the Community Structure of Soybean-Nodulating Bradyrhizobia, Appl. Environ. Microbiol., 78, 4, 1243-1250, 2012.02.
55. Hee Chung JI and Takeo YAMAKAWA, Cell wall contents and Estimation of Genetic Parameters for Polysora Rust Resistance in Tropical Maize (Zea mays L.)., J. Fac. Agr. Kyushu Univ., 56, 2, 231-236, 2011.10, [URL].
56. Takeo YAMAKAWA, Masayuki TANAKA, Masao SAKAI and Papa Saliou SARR, Genetic Grouping of Bradyrhizobium StrainsCompatible with Soybean (Glycine max L. Merr.) Harboring Rj-gene by AFLP-fingerprinting Analysis., J. Fac. Agr. Kyushu Univ., 56, 2, 223-229, 2011.10, [URL].
57. Papa SALIOU SARR, Takeo YAMAKAWA, Yuichi Saeki and Aliou GUISSE, Phylogenetic diversity of indigenous cowpea bradyrhizobia from soils in Japan based on sequence analysis of the 16S-23S rRNA Internal Transcribed Spacer (ITS) region., Systematic and Applied Microbiology, 34, 4, 285-292 , 2011.06.
58. Aung Kyaw MYINT, Takeo YAMAKAWA, Takahide ZENMYO, Hoang Thi Bich TAO and Papa Saliou SARR, Effects of organic-manure application on growth, grain yield, and nitrogen, phosphorus, and potassium recoveries of rice variety Manawthuka in paddy soils of differing fertility., Communications in Soil Science and Plant Analysis, 42, 4, 457–474, 2011.02.
59. Papa SALIOU SARR, Takeo YAMAKAWA, S. ASATSUMA, Syunsei FUJIMOTO and Masao Sakai, Investigation of endophytic and symbiotic features of Ralstonia sp. TSC1 isolated from cowpea nodules., African Journal of Microbiology Research, 4, 19, 1959-1963 , 2010.10.
60. Aung Kyaw Myinta, Takeo Yamakawa, Yoshinori Kajihara, Khin Khin Marlar Myint and Takahide Zenmyo , Application of different organic and mineral fertilizers on the growth, yield and nutrient accumulation of rice in a Japanese ordinary paddy field. , Science World Journal, 5, 2, 47-54, 2010.07.
61. Aung Kyaw Myinta, Takeo Yamakawa, Yoshinori Kajihara, Khin Khin Marlar Myint and Takahide Zenmyo , Nitrogen dynamics in a paddy field fertilized with mineral and organic nitrogen sources. , American-Eurasian J. Agric. & Environ. Sci, 7, 2, 221-231, 2010.03.
62. Hee Chung JI, Hee Bong LEE and Takeo YAMAKAWA, Major Agricultural Characteristics and Antioxidants Analysis of the New Developed Colored Waxy Corn., J. Fac. Agr. Kyushu Univ., 55, 1, 55-59, 2010.02, [URL].
63. Hoang Thi Bich THAO and Takeo YAMAKAWA, Phosphate absorption of intact komatsuna plants as influenced by phosphite. , Soil Sci. Plant Nutr, 56, 1, 133-139, 2010.02, [URL].
64. Aung Kyaw MYINT・Takeo YAMAKAWA・Takahide ZENMYO , Plant growth, seed yield and apparent nutrient recovery of rice by the application of manure and fertilizer as different nitrogen sources on paddy soils. , J. Fac. Agr. Kyushu Univ., 54, 2, 329-337, 2009.10.
65. Yadana Khin LATT・ Aung Kyaw MYINT・Takeo YAMAKAWA・Kazuo OGATA , The effects of green manure (Sesbania rostrata) on the growth and yield of rice. , J. Fac. Agr. Kyushu Univ., 54, 2, 313-319, 2009.10.
66. Yuichi Saeki, Hiroko Oguro, Isao Akagi, Takeo Yamakawa and Akihiro Yamamoto, Diversity of internal transcribed spacer regions between 16S-23S rRNA genes among the three copies of soybean-nodulating Sinorhizobum strains. , Soil Sci. Plant Nutr, 55, 5, 627-633, 2009.10, [URL].
67. Takeo Yamakawa, Mayuko Yamano and Motoki Ikeda 2009 Effect of applied position of crushed fibrous-banboo and nitrogen fertilizer on yield and N, P, K accumulation of soybean (Glycine max L. Merr.) cv. Fukuyutaka. Jpn. J. Soil Sci. Plant Nutr., 80(4), 379-386


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68. Hoang Thi Bich THAO・Takeo YAMAKAWA・Katsuhiro SHIBATA, Effect of phosphite-phosphate interaction on growth and quality of hydroponic lettuce (Lactuca sativa L.), J. Plant Nutr. Soil Sci, 172, 3, 385-392, 2009.06.
69. Hoang Thi Bich THAO・Takeo YAMAKAWA, Phosphite (Phosphorous acid): Fungicide, fertilizer or bio-stimulator?, Soil Sci. Plant Nutr, 55, 2, 228-234, 2009.04.
70. Papa SALIOU SARR・Takeo YAMAKAWA・Syunsei FUJIMOTO・Yuichi SAEKI・Hoang Thi Bich THAO・Aung Kyaw MYINT , Phylogenic diversity and symbiotic effectiveness of root-nodulating bacteria associated with cowpea in the South-West area of Japan, Microbes Environ., 24 (2), 105-112 , 2009.03.
71. Takeo Yamakawa, Mayuko Yamano and Motoki Ikeda 2009 Effect of K and Mulching of Crushed Fibrous-Bamboo on Growth and Yield of Soybean (Glycine max L. Merr.) cv. Fukuyutaka. Jpn. J. Soil Sci. Plant Nutr., 80(1), 7-13


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72. Sarr P. S.,, Khouma M., Sene M., Guisse A., Badiane A. N. and Yamakawa T., Effect of natural phosphate rock enhanced compost on pearl millet-cowpea cropping systems. J. Fac. Agr. Kyushu Univ., 54 (1), 29-35, J. Fac. Agr. Kyushu Univ., 54, 1, 29-35, 2009.02.
73. Jin-Woong CHO and Takeo YAMAKAWA, Comparison of Dry Matter Production and Photosynthetic Rate against Different Planting Dates between Late and Early Maturing Soybean cultivars in Paddy Field. , J. Fac. Agr. Kyushu Univ., 53 (2), 389-393, 2008.10.
74. Jin-Woong CHO , Young-Jin OH, Jung D. SO and Takeo YAMAKAWA , Evaluation of Dense Planting Adaptation Characteristics of Small seed Soybeans by Principal Components Analysis., J. Fac. Agr. Kyushu Univ., 53 (2), 385-388, 2008.10.
75. Hee Chung JI and Takeo YAMAKAWA , Inheritance of Long Husk Leaves of Maize in Recombinant Inbred lines (RILs) ., J. Fac. Agr. Kyushu Univ., 53 (2), 379-384, 2008.10.
76. Hoang Thi Bich THAO・Takeo YAMAKAWA, Growth of Celery (Apium graveolens var. dulce) as influenced by Phosphite., J. Fac. Agr. Kyushu Univ., 53 (2), 375-378, 2008.10.
77. Hoang Thi Bich THAO, Takeo YAMAKAWA, Papa Saliou SARR, and Aung Kyaw MYINT, Effects of phosphite, a reduced form of phosphate, on growth and phosphorus nutrition of spinach (Spinacia oleracea L.) ., Soil Sci. Plant Nutr, 54(5), 738-743, 2008.10.
78. Hoang Thi Bich THAO, Takeo YAMAKAWA, Katsuhiro SHIBATA, Papa Saliou SARR, and Aung Kyaw MYINT, Growth response of komatsuna (Brassica rapa var. peruviridis) to root and foliar applications of phosphite fertilizer. , Plant and Soil, 54(2), 197-203, 2008.05.
79. Ueda S., Ikeda M. and Yamakawa T. , Provision of Carbon Skeletons for Amide Synthesis in Non-nodulated Legume Roots in Response to Supply of Nitrogen Source. , Soil Sci. Plant Nutr., 54(5), 732-737, 2008.04.
80. Hirohito Tsurumaru, Takeo Yamakawa, Masayuki Tanaka and Masao Sakai, Tn5 mutants of Bradyrhizobium japonicum Is-1 with altered compatibility with Rj2-soybean cultivars., Soil Sci. Plant Nutr., 54(2), 197-203, 2008.04.
81. Koutaro Suzuki, Hiroko Oguro, Takeo Yamakawa, Akihiro Yamamoto, Shoichiro Akao and Yuichi Saeki, Diversity and Distribution of Indigenous Soybean-Nodulating Rhizobia in the Okinawa Islands, Japan., Soil Sci. Plant Nutr., 54(2), 237-246, 2008.04.
82. Takeo Yamakawa, Naoko Okuda and Kenjirou Taira, The Difference between the micronutrients content of seedling’s root and root hair in several plant species., J. Fac. Agr. Kyushu Univ., 53 (1), 33-38, 2008.02.
83. Hirohito Tsurumaru, Takeo Yamakawa, Masayuki Tanaka and Masao Saka, The efficient strategy of plasmid rescue from Tn5 mutants derived from Bradyrhizobium japonicum Is-1, based on whole genome sequence information of strain USDA110., J. Fac. Agr. Kyushu Univ., 53 (1), 27-31, 2008.02.
84. Hoang Thi Bich THAO, Thomas GEORGE, Takeo YAMAKAWA and Ladiyani Retno WIDOWATI, Effects of soil aggregate size on phosphorus extractability and uptake by rice (Oryza sativa L.) and corn (Zea mays L.) in two Ultisols from Philippines., Soil Sci. Plant Nutr., 54 (1), 148-158, 2008.02.
85. Papa Saliou SARR, Mamadou KHOUMA, Modou SENE, Aliou GUISSE, Aminata Niane BADIANE , Takeo YAMAKAWA, Effect of Pearl millet-cowpea cropping systems on nitrogen recovery, nitrogen use efficiency and biological fixation using 15N tracer technique., Soil Sci. Plant Nutr., 54 (1), 142-147, 2008.02.
86. Young-Jin OH, Kyong-Ho KIM, Jung-Gon KIM, Jin-Woong CHO and Takeo YAMAKAWA, Growth traits and sink capacity in late sown soybean cultivars with different stem lengths, J. Fac. Agr. Kyushu Univ., 52 (2), 299-305, 2007.10.
87. Hee Chung Ji, Young Il Kim, Hee Bong Lee, Jin-Woong Cho , Sung Seo and Takeo Yamakawa, Genetic Diversity of Colored Rice Lines Based on Botanical Characteristics and Simple Sequence Repeat (SSR) Markers., J. Fac. Agr. Kyushu Univ., 52 (2), 287-293, 2007.10.
88. Yuichi Saeki, Tadashi Murata, Takeo Yamakawa and Shoichiro Akao, Differentiation of soybean-nodulating Bradyrhizobium USDA strains by restriction fragment length polymorphism analysis of 23S-5S rRNA genes, Soil Sci. Plant Nutr., 53 (5), 562-567, 2007.10.
89. Takeo Yamakawa, Yoshie Nakano and Arisa Yamada Methods of purification and quantitative determination of soybean isoflavones with HPLC. Jpn. J. Soil Sci. Plant Nutr., 78, 2, 171-177


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90. Takeo Yamakawa, Yoshie Nakano, Sayuri Kajihara and Toshihiro Mochizuki Effect of filling temperature on the transition of isoflavone content in soybean plant. Jpn. J. Soil Sci. Plant Nutr., 78, 1, 39-44

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91. Takeo Yamakawa, Yoshie Nakano, Arisa Yamada, Sayuri Kajihara and Toshihiro Mochizuki Difference by soybean cultivar and harvest year of isoflavone content in soybean seed. Jpn. J. Soil Sci. Plant Nutr., 78, 1, 33-38



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92. Yamakawa T. and K. Taira Effect of inoculation of Bradyrhizobium japonicum withdifferent compatibility on deformation of root hairs in soybean plant. Sci. Bull. Fac. Agri. Kyushu Univ., 61, 2, 177-183.
93. Fukushima, Y. and T. Yamakawa Effect of difference of inoculation methods of Bradyrhizobium japonicum USDA110 on production of soybean (Glycine max L. Merr.). Sci. Bull. Fac. Agri. Kyushu Univ., 61, 2, 171-176.
94. Saeki Y., Aimi N., Tsukamoto S., Yamakawa T., Nagatomo Y. and Akao S., Diversity and Geographical Distribution of Indigenous Soybean-Nodulating bradyrhizobia in Japan., Soil Science and Plant Nutrition, 52, 4, 418-426, 2006.08.
95. Tanaka K., Shimizu T., Zakari M., Njoloma J., Saeki Y., Sakai M., Yamakawa T., Minamizawa K. and Akao S., Incorpration of a DNA sequence encoding Green fluorescent protein (GFP) into endphytic diazotrop from sugarcane and sweet potato and the colonizing ability of these bacteri in Brassica oleracea., Microbes Environ, 21, 2, 122-128, 2006.07.
96. Ji H. C., Cho J. -W. and Yamakawa T. , Diallel Analysis of Plant and Ear Heights in Tropical Maize (Zea mays L.). , J. Fac. Agr. Kyushu Univ, 51, 2, 233-238, 2006.02.
97. Cho J.-W., Ji H. C. and Yamakawa T., Comparison of Photosynthetic Response of Two Soybean Cultivars to Soil Flooding. , J. Fac. Agr. Kyushu Univ, 51, 2, 227-232, 2006.02.
98. Cho J.-W. and Yamakawa T., Tolerance Differences among Small Seed Soybean Cultivars against Excessive Water Stress Conditions. , J. Fac. Agr. Kyushu Univ, 51, 2, 195-199, 2006.02.
99. Cho J.-W. and Yamakawa T., Effects on Growth and Seed Yield of Small Seed Soybean Cultivars of Flooding Conditions in Paddy Field., J. Fac. Agr. Kyushu Univ, 51, 2, 189-193, 2006.02.
100. Saeki Y., Kaneko A., Hara T., Suzuki K., Yamakawa T., Minh N. T., Nagatomo Y., and Akao S., Phylogenetic Analysis of Soybean-Nodulating Rhizobia Isolated from Alkaline Soils in Vietnam., Soil Science and Plant Nutrition, 10.1111/j.1747-0765.2005.tb00143.x, 51, 7, 1043-1052, 51, 7, 1043-1052, 2005.12.
101. The condition of cultivation for the root hair of the soybean (Glycine max L. Merr.)seedlings to appear favorably, and the separation of the root hair
Takeo Yamakawa and Kenjirou Taira, Japnese Journal of soil Science and Plant Nutrition, 76 (4), 471-475.
102. Yamakawa T., Hussain A.K.M.A., and Ishizuka J., Soybean preference for Bradyrhizobium japonicum for nodulation. Occupation of Serogroup USDA110 in nodules of soybean plants harboring various Rj-genes grown in a field., Soil Science and Plant Nutrition, 49, 6, 835-841, 49, 6, 835-841, 2003.12.
103. Yamakawa T., Ikeda T., and Ishizuka J., Effects of CO2 Concentration in Rhizosphere on Nodulation and N2 Fixation of Soybean and Cowpea, Soil Science and Plant Nutrition, 50, 5, 713-720, 50, 5, 713-720, 2004.10.
104. Manolov, I.G., Ikeda, M. and Yamakawa T., Effect of methods of nitrogen application on nitrogen recovery from 15N-labeled urea applied to paddy rice (Oryze sativa L.)., J. Fac. Agr. Kyushu Univ, 48, 1-2, 1-11, 48, 1-2, 1-11., 2003.10.
105. Yamakawa T. and Ishizuka J., Effect of nodulation with Bradyrhizobium japonicum and Shinorhizobium fredii on xylem sap composition of Peking (Glycine max L. Merr.)., Soil Sci. Plant Nutr., 48, 4, 521-527, 48, 4, 521-527, 2002.08.
106. Variental differnces in the effects of flooding on the nodule growth, nitrogen fixing activity in soybean. Shao-H. Zheng, Osamu Kusuda, Hirosaki Makamoto, Takeo Yamalkawa, Tadahiko Furuya and Masataka Fukuyama, J. Fac. Agr. Kyushu Univ. , 56, 3139-144, 2002.
107. Variental differnces in the effects of submerging on the nodulation, nitrogenase activity of root nodule in soybean. S.-H. Zheng, O. Kusuda, H. Makamoto, T. Yamalkawa, T. Furuya and M. Fukuyama, Jpn. J. Crop Sci., 70, 325-326, 2001.
108. Yamakawa T., Shirai T., and Ishizuka J., Effects of Symbiosis with Rhizobium fredii on Transport of Fixed Nitrogen in the Xylem of Soybean Plants., Soil Sci. Plant Nutr, 46, 4, 885-892, 46, 4, 885-892, 2000.01.
109. Yamakawa T., Eriguchi M., Hussain A.K.M.A., and Ishizuka J., Soybean preference for Bradyrhizobium japonicum for nodulation. Nodulation by Rj2Rj3Rj4-genotypes isolated from the progenies of cross of soybean cvs. IAC-2 (Rj2Rj3) and Hill (Rj4)., Soil Sci. Plant Nutr., 45, 2, 461-469, 45, 2, 461-469, 1999.01.
110. Md. Mizanur R. Bhuiyan, Takeo Yamakawa, Masamichi Kikuchi and Motoki Ikeda, Irrigation of Secondary Sewage Effluent: Salinity and Nitrogen Effects on Growth and Nitrogen Fixation of Nodulated and Non-nodulated Soybeans., J. Fac. Agr. Kyushu Univ., 42, 3-4, 273-280, 43, 4, 273-280., 1998.01.
111. Saeki Y., Yamakawa T., Ikeda M., and Ishizuka J., Nodule formation and distribution of Rj2Rj3-genotype soybean infected with Bradyrhizobium japonicum., J. Fac. Agr. Kyushu Univ., 43, 3-4, 317-326, 43, 3-4, 317-326, 1999.01.