Kyushu University Academic Staff Educational and Research Activities Database
List of Presentations
Miki Nakao Last modified date:2019.09.08

Professor / Division of Molecular Bioscience / Department of Bioscience and Biotechnology / Faculty of Agriculture


Presentations
1. Rosli N, Yamamoto A, Nagasawa T, Somamoto T, Nakao M, Functional analysis of two complement C4 isotypes of carp using recombinant proteins, 日本水産学会秋季大会, 2018.09.
2. Rapid purification of carp complement component C5 utilizing a specific immunoadsorbent.
3. Praparation of anti-zebrafish C5a to elucidate roles of complement C5a in inflammatory reaction of fish.
4. Nakao M, Iwanaga S, Nagasawa T, Somamoto T, Evolutionary implication on the function of the classical pathway of the complement system, Asian Invertebrate Immunity Symposium, 2018.09.
5. Nagasawa T, Somamoto T, Nakao M, Type I IFN production by carp thrombocytes as professional antiviral leukocytes, like mammalian plasmacytoid dendritic cells, via IRF signaling pathway, Asian Invertebrate Immunity Symposium, 2018.09.
6. Nakao M, Iwanaga S, Nagasawa T, Somamoto T, Phylogenetic inference on functions of the classical complement pathway in bony fish, 14th Congress of International Society of Developmental and Comparative Immunology, 2018.06, Bony fish is one of the ancestral vertebrates that possess immunoglobulins, on which the classical pathway of complement system relies for target recognition. To access the role of the classical pathway in bony fish complement, we have analyzed the hemolytic reaction and C3b-deposition on the target surface of the common carp (Cyprinus carpio) complement, using carp serum immunochemically depleted of factor D (Df), a serine protease responsible for activation of the alternative pathway, an activation and amplification cascade that bypasses the classical pathway. To our surprise, the Df-depletion abolished hemolytic activities of the serum both through the classical and alternative pathways, when assayed using antibody-sensitized and non-sensitized sheep and rabbit erythrocytes. However, a low level of C3b-deposition, probably resulted from the classical pathway activation in the absence of Df was demonstrated on various target surfaces by flow cytometry and ELISA. In addition, Df-depletion inhibited C5-deposition essential for the cytotoxic complex formation on the target cells. The C3b-bound erythrocytes were hemolyzed by serum but easily lysed by Mg-EGTA-serum, probably triggering the alternative pathway amplification more efficiently than unbound erythrocytes. These results, taken together, suggest that an ancestral classical pathway alone lacks C5-activating ability but tags target cells with a small amount of C3b, which triggers enhanced C3-activation by the alternative pathway, leading to completion of the terminal cytolytic pathway..
7. Nagasawa T, Somamoto T, Nakao M, Viral ligands recognition of carp thrombocytes as natural type I interferon producing cells, 14th Congress of International Society of Developmental and Comparative Immunology, 2018.06.
8. Cytotoxicity of T cells against a prasite Ichthyophthirius multiliis in ginbuna crucian carp.
9. Cytotoxicity of T cells against a prasite Ichthyophthirius multiliis in ginbuna crucian carp.
10. Nakao M, Noguchi M, Akahoshi S, Nagasawa T, Somamoto T, Functional diversity of two C7 isotypes in bony fish, a primitive vertebrate model, European Meeting on Complement in Human Disease, 2017.09, [URL].
11. Prakash H, Motobe S, Nagasawa T, Somamoto T, Nakao M, Functional analysis of Tecrem, a CD46-like complement regulatory protein, on epithelial cells in the common carp, The JSFS 85th Anniversary-Commemorative International Symposium, 2017.09.
12. Development of KHV disease in ginbuna crucian carp by suppression of cellular immunity..
13. Cytotoxicity of T cells against a prasite Ichthyophthirius multiliis in ginbuna crucian carp.
14. Functional analysis of two C7 isotypes of carp complement system..
15. Harsha Prakash, Shiori Motobe, Takahiro Nagasawa, Tomonori Somamoto, Miki Nakao, Expression and homeostatic functions of Tecrem, a CD46-like complement regulatory protein on epithelial cells in bony fish., 26th International Complement Workshop, 2016.09, Mammalian CD46 has been reported as a multitasking immune modulator, which regulates complement activation on host cells, T cell-mediated adaptive responses, and wound repair by involving epithelial cells. It is of particular interest to explore the evolutionary path of such versatile functions of CD46.

Our group has identified a CD46-like molecule, termed teleost complement regulatory membrane protein or Tecrem, in a few cyprinid fish species and has shown its regulatory function on complement activation at the protein level. Furthermore, we have also found that Tecrem expressed on T cells modulates mitogen-induced T cell proliferation in ginbuna crucian carp, indicating that modulation of adaptive immunity is one of the evolutionarily conserved functions of the CD46-like complement regulator. In the present study, we have explored a homeostatic role of Tecrem in the maintenance of fish epithelium, by analyzing expression behavior of Tecrem on an epithelial cell line (KF-1) derived from carp fin.

Flow cytometric analysis of Tecrem expression on KF-1 using anti-carp Tecrem monoclonal antibody (MAb) (1F12) suggested that Tecrem expression may be affected by cell aggregation and adhesion. Fluorescent microscopic observation and ELISA-based assay for Tecrem also indicated a role of Tecrem in the adhesion of KF-1 to the surface of culture media. Furthermore, 1F12 MAb deposited on the culture plate significantly enhanced an early stage of cell adhesion process of KF-1.

Towards further functional analyses of carp Tecrem, we have prepared recombinant Tecrem proteins in the bacterial expression system. Among the four short consensus repeat (SCR) modules making up the extracellular domains of Tecrem, the N-terminal two SCRs (rSCR1-2) and the C-terminal two SCRs (rSCR3-4) were separately expressed as 6xHis-tagged soluble protein using pCold-I vector and Origami B strain. Interaction of the two recombinant domains with carp C3 isotypes and their inhibition of carp complement activation cascades are currently analyzed in parallel with raising polyclonal antibodies, for the clarification of functional modules and their mode of action.
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16. Kazuki Yoshioka, Yoko Kato-Unoki, Takahiro Nagasawa, Tomonori Somamoto, Miki Nakao, Carp properdin: structural and functional diversity of two isotypes., 26th International Complement Workshop, 2016.09, The alternative pathway (ACP) of the complement system is an antibody-independent activation pathway, in which properdin (Pf) has been known as a positive regulator of the activation and a possible pattern-recognition molecule to trigger ACP activation. Teleost complement system has a striking feature that some of its components are diversified into multiple isoforms with different functions. However this diversity is less characterized for teleost Pf, especially at the protein level. The present study was aimed at elucidating isotypic diversity and functional differentiation of Pf in the common carp. Molecular cloning of carp Pf revealed two distinct full-length cDNA sequences, CaPf1 and CaPf2, that predicts mature proteins composed of seven thrombospondin type1 domains (TSP0-TSP6), sharing 77% amino acid sequence identity. Genomic Southern hybridization suggested that CaPf1 and CaPf2 are encoded by single each gene in carp genome. Real-time quantitative PCR indicated that expression level of CaPf1 is most abundant in the spleen, whereas CaPf2 was detected mainly in head kidney and renal kidney. Rabbit antibodies were raised against their recombinant proteins corresponding to TSP4-6 domains. Western blotting using anti-CaPf1 and anti-CaPf2 revealed that CaPf1 and CaPf2 are mainly present as a hexamer of polypeptide with molecular weights of 49,000 and 48,000, respectively, in carp serum. Interestingly, CaPf1 and CaPf2 showed different spectra of binding to various microbes, suggesting their functional diversity..
17. Gene knockout of myeloperoxidase in zebrafish that lacks adaptive immunity.
18. Effects of dietary soybean meal protein on antibody response in fish.
19. Kazuki Yoshioka, Yoko Kato-Unoki, Tomonori Somamoto, Miki Nakao, Structural and functional diversity of properdin isotypes in the common carp complement system. , 13th Congress of the International Society for Developmental and Comparative Immunology, 2015.06, The alternative pathway (ACP) of the complement system is an antibody-independent activation pathway, in which properdin (Pf) has been known as an essential positive regulator of the activation and possibly as a pattern-recognition molecule to trigger ACP activation. Teleost complement system has a striking feature that some of its components are diversified into multiple isoforms with different functions. However this diversity is less characterized for teleost Pf, especially at the protein level. The present study was aimed at elucidating isotypic diversity and functional differentiation of Pf in the common carp. Molecular cloning of carp Pf revealed two distinct full-length cDNA sequences, CaPf1 and CaPf2, that predicts mature proteins composed of seven thrombospondin type1 domains (TSP0-TSP6), sharing 77% amino acid sequence identity. Genomic Southern hybridization suggested that CaPf1 and CaPf2 are encoded by single each gene in carp genome. Real-time quantitative PCR indicated that expression level of CaPf1 is most abundant in the spleen, whereas CaPf2 was detected mainly in head kidney and renal kidney. Rabbit antibodies were raised against their recombinant proteins corresponding to TSP4-6 domains. Western blotting using anti-CaPf1 and anti-CaPf2 revealed that CaPf1 and CaPf2 are mainly present as a hexamer of polypeptide with molecular weights of 49,000 and 48,000, respectively, in carp serum. Interestingly, CaPf1 and CaPf2 showed different spectra of binding to various microbes, suggesting their functional diversity..
20. Immune-regulatory mechanism in rag1-deficient zebrafish.
21. Effect of soy bean meal feeding on the complement system of rainbow trout.
22. Indriyani Nur, 原田光利, 杣本 智軌, 中尾 実樹, 中村亮太, 辻倉正和, Characterizations of membrane-bound complement regulatory protein in Ginbuna Crucian Carp Carassius auratus langsdorfii, 日本比較免疫学会学術集会, 2013.08.
23. Carp thrombocytes are activated by other leukocytes to show phagocytosis..
24. Monoclonal antibody preparation and expression analysis of ginbuna CD83, a dendritic cell marker..
25. Takahiro Nagasawa, Chihaya Nakayasu, Tomomasa Matsuyama, Aja M. Rieger, Daniel R. Barreda, Tomonori Somamoto, Miki Nakao, Phagocytosis and bactericidal abilities of teleost thrombocytes, 12th Congress of International Society of Developmental and Comparative Immunology, 2012.07, Thrombocytes have been recognized as haemostatic cells in non-mammalian vertebrates. Unlike mammalian
platelets, thrombocytes are nucleated cells with a lymphocyte-like morphological feature, and possible
involvement of thrombocytes in innate immune function has been considered in addition to the haemostatic
function. In the present study, we report phagocytic abilities of some teleost thrombocytes. Using a monoclonal
antibody specific for thrombocytes of common carp (Cyprinus carpio), thrombocytes were isolated from
peripheral blood and examined for expression of various immune-related genes, resulting in detection of
significant level of lysozyme, iNOS and MHC class II using RT-PCR. Upon flow cytometry-based phagocytosis
assay, a number of thrombocytes ingested fluorescent latex particles (0.5 μm, 1 μm, 2 μm and 3 μm), bacteria
(Escherichia coli) and zymosan particles. Phagocytosis by the thrombocytes was also confirmed by fluorescent
microscopy and transmission electron microscopy, which revealed internalization of these particles into
thrombocytes. We also observed that thrombocytes of the olive flounder (Paralichthys olivaceus) had similar
phagocytic behavior. These data indicate that thrombocytes of those species are potent phagocytes, suggesting
that those phagocytic characteristics of thrombocytes are widely conserved in teleosts. We also assessed a
phagolysosome formation ability of teleost thrombocytes against the ingested pathogens, for detecting
intracellular bactericidal activities of those thrombocytes. By using an ImageStream multi-spectral flow
cytometer, we assessed phagolysosome fusion of goldfish (Carassius auratus) thrombocytes. On this analysis
we detected that lysosomes of these thrombocytes visualized with fluorescent dextran were co-localized with the
ingested small beads and formed a ring around large beads like other typical phagocytes. Overall, the results
indicate that teleost thrombocytes have dual functions as not only haemostatic cells but also as phagocytic
immune cells against microbial infections..
26. Haruka Tsukamoto, Yutaka Fukuda, Tomonori Somamoto, Miki Nakao, Functions of CD8-positive and CD4-positive lymphocytes against virus-infection in ginbuna crucain carp, 12th Congress of International Society of Developmental and Comparative Immunology, 2012.07, Two serotypes of Streptococcus parauberis, pathogens of streptococcosis in Japanese flounder (Paralichthys
olivaceus), have caused severe losses of the livestock, due to the lack of effective vaccine and its resistance to
antibiotics approved for flounder culture. These strains show different virulence, but their mechanisms of their
pathogenicity are totally unknown, and identification of virulence factors are needed for effective vaccine
development. The present study, therefore, was aimed at clarifying effect of S. parauberis culture supernatant on
flounder. Since the diseased flounder shows anaemia, we examined the effects of S. parauberis on flounder
peripheral blood. As a result, S. parauberis -injected flounder showed decreased numbers of peripheral
erythrocytes, suggesting that S. parauberis may produce a haemolytic factor. To characterize the haemolytic
factor, a supernatant of S. parauberis culture (25°C for 48h in Todd Hewitt broth) was mixed with sheep
erythrocytes, resulting in significant level of haemolysis. The haemolytic factor was stable on heating at 100°C
for 10 min, and passed through an ultrafiltration membrane with a 5 kDa cut off limit (Amicon Ultra15),
indicating that the factor is not proteinaceous haemolysin but a heat-resistant low molecular mass substance. We
also investigated the effects of the culture supernatant on flounder leukocytes. Peripheral leukocytes were
separated from flounder and carp using Percoll discontinuous density gradient centrifugation and cultured with
the supernatant in 96-well plate. The stimulated flounder cells showed agglutination and significant proliferation
as assayed by BrdU uptake, while carp cells did not. These results suggest that S. parauberis secrets a mitogen
specific for flounder leucocytes. Characterization of the mitogen and resulting leucocyte response is in progress..
27. Tomokazu Yamaguchi, Kazufumi Takamune, Masakazu Kondo, Yukinori Takahashi, Miki Nakao, Yoko Kato-Unoki, Tamotsu Fujii, Identification of Pattern Recognition Molecules in Hagfish Complement System, 12th Congress of International Society of Developmental and Comparative Immunology, 2012.07, All extant jawed vertebrates share a common adaptive immune system in which immunoglobulin domain-based
molecules act as antigen receptors. On the other hand, jawless vertebrates, lamprey and hagfish, use variable
lymphocyte receptors composed of leucine-rich repeat cassettes for antigen recognition. Since invertebrates and
primitive chordates do not have an adaptive immune system, the immune system seems to change dramatically
in the course of evolution from primitive chordates to gnathostomes. From a phylogenic perspective of defence
mechanisms, previously we found complement C3 and mannose-binding lectin-associated serine protease 1
(MASP-1) in hagfish and suggested the involvement of lectin pathway in hagfish innate immune system. In this
study, we focused on the pattern recognition molecules in the hagfish, Eptatretus burgeri, and tried to purify
them from serum by affinity chromatography. When the serum was treated with GlcNAc-agarose, followed by
successive elution of the binding molecules with GlcNAc and EDTA, mainly four proteins (31 kDa, 27 kDa, 26
kDa, and 19 kDa) and 26 kDa protein were detected in the GlcNAc-eluate and EDTA-eluate, respectively.
Collagenase treatment showed the presence of collagen-like domain only in the 26 kDa protein in the EDTAeluate.
Since common pattern recognition molecules such as mannose-binding lectins possess collagen-like
domains, we examined the entity of the 26 kDa protein by sequencing its N-terminal amino acids and cDNA
obtained by 3’ and 5’ RACE methods, and identified it as a member of C1q family. Herein, it will be referred to
as hagfish C1q (hagC1q). Western blot analyses using anti-hagC1q, MASP-1, and complement C3 antibodies
showed that hagC1q associated with MASP-1 and complement C3 in the serum and had binding ability to
Escherichia coli as a divalent cation-dependent manner. These results suggest that hagC1q plays an important
role in hagfish innate immune system..
28. Vo Kha Tam, Chie Okura, Masakazu Kondo, Tomonori Somamoto, Miki Nakao, Isotypic diversity in the ontogenetic expression of the complement component in the common carp (Cyprinus carpio), 12th Congress of International Society of Developmental and Comparative Immunology, 2012.07, Isotypic diversity of complement components is a striking feature of the teleost complement system. As a first
line of innate defence, the complement system has been considered as an important clue to humoral defence in
early development however functional diversity of the isotypes during teleost ontogeny is poorly understood.
The present study aimed at clarifying comprehensive picture of ontogenetic expression of the diversified
complement component isotypes in carp. Real-time quantitative PCR detected embryonic expression of C1r/s,
MASPs, factor B/C2, C3, C4, C5, C6, C7, C8, C9, and factor I. A remarkable difference in the expression time
course was noted between the isotypes in C3, C4, and C5. Especially, teleost-specific isotypes of C3 and C4
(non-histidine-type) started around hatching, in contrast to evolutionarily common isotypes (histidine-type),
which showed much earlier expression. Whole-mount in situ hybridization of carp embryos revealed some
difference in embryonic expression sites of two major C3 isotypes (C3-H1 and C3-S) in addition to common
expression sites such as the yolk syncytial layer. The temporal and spatial differences in expression among the
isotypes suggest that the isotypes are functionally differentiated in teleost early development..
29. Miki Nakao, Masakazu Tsujikura, Satoko Ichiki, Vo Kha Tam, Tomonori Somamoto, Structural and functional diversity of the complement system, an innate immune factor, in fish, 8th International Congress of Comparative Physiology and Biochemistry, ICCPB-Nagoya, 2011.06.
30. Miki Nakao, Satoko Ichiki, Masakazu Tsujikura, Vo Kha Tam, Tomonori Somamoto, Complement system in teleost fish: Isotypic diversity in pathogen-recognition, activation cascade, and ontogeny, Comparative Immunology and Pathology Workshop Edmonton 2011, 2011.05.
31. Takahiro Nagasawa, Chihara Nakayasu, Tomomasa Matsuyama, Tomonori Somamoto, Miki Nakao, Phagocytic activities of carp (Cyprinus carpio ) thrombocytes, Comparative Immunology and Pathology Workshop Edmonton 2011, 2011.05.
32. Phagocytic function of fish thrombocytes.
33. Diversity in target-recognition of carp serum collectins related to the lectin pathway of the complement
Akito Ichiki, Haruka Tsukamoto, Tomonori Somamoto, and Miki Nakao.
34. Functional diversity of the complement component isotypes in bony fish innate immunity: a model study using the common carp.
Nakao, M., Ichiki, S., Mutsuro, J., Tsujikura, M., and Somamoto, T..
35. Functional differentiation of C7 isotypes in the common carp complement system.
36. Recognition specificity of carp serum lectins associated with the complement system.
37. Structural diversity and functional differentiation of the complement components in bony fish.
Miki Nakao.
38. Subcomponent structure of the first complement component (C1) in the common carp.
Takashi Ichii, Masakazu Tsujikura, Tomonori Somamoto, Yoko Kato-Unoki, Shin-ichi Kato, Michiyasu Yoshikuni, Miki Nakao.
39. Expression pattern of complement component C3 isotypes during early development of the common carp.
Vo Kha Tam, Chie Okura, Tomonori Somamoto, Miki Nakao.
40. Functional differentiation of complement C3 isotypes in the teleost immune defense.
Satoko Ichiki, Yoko Kato-Unoki, Tomonori Somamoto, Miki Nakao.
41. cDNA cloning and expression analysis of Fas ligand in ginbuna crucian carp.
42. Phagocytic ability of carpo thrombocytes.
43. Subcomponent structure of the first complement component (C1) in the common carp.
44. Cloning, purification and characterization of zymosan-binding proteins in Nile Tilapia (Oreochromis niloticus)
Soha Gomaa, Tomonori Somamoto and Miki Nakao.
45. Complement components constituting the classical activation pathway, an antibody-dependent activation cascade of the complement system..
46. cDNA cloning of four lineages of MHC class I genes in a cell line from clonal ginbuna crucian carp, and their expression in the cell line infected with CHNV.
47. Identification of a novel membrane-bound regulator of complement activation in carp and zebrafish.
48. Diversity of carp serum lectins involved in the lectin pathway of the complement activation..
49. Identification and expression analysis of two isotypes of carp complement component C7.
50. Structural and functional diversity of the complement components in bony fish: implication for innate immune defense.
51. Structure and regulation of the isotypic genes of the complement factor B in the common carp.
52. Identification of a novel complement regulatory factor in bony fish.
53. Structure and regulation of the isotypic genes of the complement factor B in the common carp.
54. Antibacterial substances from bony fish: structure, function, and application.
55. Functional analysis of carp C3 isotypes using monoclonal antibodies.
56. Identification and expression analysis of a novel interleukine 8 (IL-8)-like CXC chemokine in carp (Cyprinus carpio).
57. Expressed sequence tag analysis of kidney and spleen from ginbuna crucian carp,
Carassius auratus langsdorfii
.
58. cDNA cloning of MHC class I and MHC-related genes in a cell line from clonal ginbuna crucian carp.
59. Expression analysis of complement component isotypes during early development of the common carp.
60. Identification of Carp Complement Component C1.
61. Functional analysis of carp C3 isoforms using monoclonal antibodies.
62. Identification of novel regulators of complement activation in fish.
63. Recombinant carp complement component C4 and C5: Expression and functional analysis.
64. Identification and expression analysis of zebrafish complement component genes.
65. Inference of bio-defensive functions of the complement system based on its gene expression pattern..
66. EST Analysis of mRNAs Expressed in Gill and Intestine of Carp (Cyprinus carpio. L).
67. Identification of the genes encoding complement components from the zebrafish genome database.
68. Molecular cloning of C3d, a fragment from complement component C3 with possible adjuvant activity, from marine cultured fish.
69. Molecular cloning of a complement-regulatory factor from zebrafish.
70. Molecular cloning of a novel complement-regulatory factor from the common carp.
71. Molecular cloning of C1q A, B and C chains from common carp.
72. DIVESIFICATION OF COMPLEMENT COMPONENT ISOTYPES IN THE COMMON CARP: EXPRESSION PATTERN AND SOME FUNCTIONAL ASPECTS.
73. MOLECULAR CLONING AND CHARACTERIZATION OF CD4 IN GINBUNA CRUCIAN CARP.
74. Molecular cloning and characterization of the complement C1q A, B, and C chains in common carp .
75. THE COMPLEMENT SYSTEM IN INVERTEBRATES AND LOWER VERTEBRATES.
76. JADCI: EVOLVING THROUGH ANNUAL MEETINGS.
77. Complement component-encoding genes identified from the zebrafish genome database..
78. Functional analysis of factor I from carp complement.
79. Cyprinid fish as model animals for fish immunological research.
80. Diversity of alpha-2-macroglobulin in rainbowtrout.
81. In silico cloning of zebrafish complement components.
82. Effect of the complement C3d fragment on the antibody response in the common carp.
83. cDNA cloning of grass carp complement components.
84. Two MBL-like lectins functioning in the lectin pathway of carp complement.
85. An MBL-MASP2 complex that functions in the lectin pathway of a bony fish, the common carp (Cyprinus carpio).
86. Diversity of the mannose-binding lectin family in the lectin pathway of carp complement.
87. Nakao M, Maeda H, Hatanaka D, Matsushita M, Nakata M, Fujita T, Yano T.
Purification and cloning of the mannose-binding lectin from the common carp.
Japanese Association of Developmental and Comparative Immunology,
Tokyo, August 2003..
88. Katayose Y, Nakao M, Matsushita M, Fujita T, Yano T.
cDNA cloning of a C1q-like gene from the common carp.
Japanese Association of Developmental and Comparative Immunology,
Tokyo, August 2003..
89. Nakao M, Miura C, Ito S, Nakahara M, Okumura K, Yano T
Generation of a complement C3 fragment equivalent to mammalian C3d and detection of its receptor in the common carp (Cyprinus carpio).
35th Complement Symposium, Kumamoto, July 2003..
90. Nakahara M, Nakao M, Mutsuro J, Yano T.
Functional analysis of carp complement factor B/C2 isotypes.
Symposium of Japanese Society for Aqua-Genome, Nagasaki, September 2002..
91. Nakao M, Mutsuro J, Yano T.
Diversity of the complement-encoding genes in bony fish and its biological implication.
Symposium of Japanese Society for Aqua-Genome, Nagasaki, September 2002..
92. Nakao M, Hisamatu S, Nakahara M, Mutsuro J, Kato Y, Yano T.
Molecular cloning of the complement regulatory factor I from the common carp.
Japanese Association of Develpmental and Comparative Immunology, Nagoya, August 2002..
93. Nakao M, Nakahara M, Harada H, Mutsuro J, Kato Y, Yano T.
Linkage analysis and functional diversity of the complement factor B/C2 isotypes in the common carp.
39th Complement Symposium, Tokyo, July 2002..
94. Nakao M, Yano T.
Cytokine receptors and complement receptors in bony fish.
Symposium of Japanese Society for Fisheries Science, Nara, April 2002..
95. Yano T, Nakao M.
The complement system of bony fish.
Symposium of Japanese Society for Fisheries Science, Nara, April 2002..
96. Nakao M.
A strategy for host defense of bony fish: diversity of the complement system.
A Joint Symposium of the Develpmental and Comparative Immunology, Comparative Endocrinology, and Comparative Biochemistry, Tokyo, December 2001..
97. Nakahara M, Nakao M, Yano T.
Expression of recombinant protein of carp complement factor B/C2.
Japanese Society of Fish Pathology, Miyazaki, October 2001..
98. Miura C, Nakao M, Mutsuro J, Yano T.
Generation of a C3d fragment from carp C3.
Japanese Society of Fish Pathology, Miyazaki, October 2001..
99. Kato Y, Nakao M, Mutsuro J, Yano T.
cDNA cloning of carp complement C5.
38th Complement Symposium, Kyoto, August 2001..
100. Shimozono M, Kodama H, Moritomo T, Nakao M, Yano T, Tomana M, Nakanishi T.
Effect of IgM isoforms on phygocytic activity of carp neutrophils.
Japanese Society for Bio-Defense, Kyoto, August 2001..
101. Nakao M, Mutsuro J, Tanaka N, Kato Y, Yano T.
Diversity and evolution of the thioester-containing proteins in the common carp.
Japanese Association for Developmental and Comparative Immunology, Sapporo, July 2001..
102. Nakao M, Nakahara M, Bayne CJ, Fujiki K, Yano T.
cDNA cloning of the complement component C2 from bony fish.
Japanese Society for Fisheries Science, Tokyo, April 2001..
103. Nakao M, Matsumoto M, Nakazawa M, Fujiki K, Yano T.
Diversity of carp complement factor B/C2.
37th Complement Symposium, Osaka, August 2000..
104. Miura C, Nakao M, Kimura M, Yano T.
Detection of carp CR3 beta-subunit using anti-peptide antibodies.
37th Complement Symposium, Osaka, August 2000..
105. Kimura M, Fujiki K, Nakao M, Shin DH, Yano T.
cDNA cloning of carp leukocyte integrin molecules.
Japanese Association for Developmental and Comparative Immunology, Tokyo, July 2000..
106. Nakao M, Osaka K, Kato Y, Yano T.
Molecular cloning of the complement component C1r/C1s from the common carp.
Japanese Association for Develpmental and Complarative Immunology, Tokyo, July 2000..
107. Nakao M, Shimozono M, Kodama H, Moritomo T, Nakanishi T, Yano T.
Purification and functional analysis of carp IgM isoforms.
Japanese Society for Fisheries Science, Tokyo, April 2000..
108. Nakao M, Matsumoto M, Mutsuro J, Kimura M, Miura C, Fujiki K, Yano T.
Cloning and expression analysis of a novel complement factor B/C2 isotype from the common carp.
Japanese Society for Fish Pathology, Tokyo, April 2000..
109. Nakao M, Osaka K, Mutsuro J, Kimura M, Yano T.
cDNA cloning of the complement component C1r/C1s from the common carp.
Japanese Society for Fisheries Science, Tokyo, April 2000..
110. Tanaka N, Mutsuro J, Nakao M, Yano T.
cDNA cloning of the complement C4 isotypes (C4A, C4B) from the common carp.
Japanese Society for Fisheries Science, Tokyo, April 2000..
111. Kato Y, Mutsuto J, Nakao M, Yano T.
Monoclonal antibodies against carp complement component C5.
Japanese Society for Fish Pathology, Tokyo, April 2000..
112. Kimura M, Fujiki K, Nakao M, Shin DH, Yano T.
cDNA cloning and gene analysis of the C3 receptor beta-subunit from the common carp.
Japanese Association for Developmental and Comparative Immunology, Fukuoka, Augusut 1999..
113. Nagai T, Nakao M, Kato Y, Mutsuro J, Kimura M, Fujiki K, Yano T.
Molecular cloning and gene analysis of the MASP isoforms from the common carp.
Japanese Association for Developmental and Comparative Immunology, Fukuoka, Augusut 1999..
114. Mutsuro J, Nakao M, Kato Y, Fujiki K, Yano T.
Isolation and identification of carp complement C3 isoforms.
Japanese Association for Developmental and Comparative Immunology, Fukuoka, Augusut 1999..
115. Nakao M, Mutsuro J, Totsuka S, Tanaka N, Kato Y, Yano T.
Purification and cloning of the complement component C4 from the common carp.
38th Complement Symposium, Tokyo, June 1999..
116. Fujiki K, Shin DH, Nakao M, Yano T.
cDNA cloning of cytokines from the common carp using the SSH technique.
Japanese Association for Developmental and Comparative Immunology, Tochigi, August 1998..
117. Shin DH, Fujiki K, Nakao M, Yano T.
cDNA cloning of NKEF from the commmon carp.
Japanese Association for Developmental and Comparative Immunology, Tochigi, August 1998..
118. Nakao M, Mutsuro J, Fujiki K, Yano T.
Molecular cloning of multiple C3 cDNA from the common carp.
35th Complement Symposium, Osaka, July 1998..