|Miki Nakao||Last modified date：2013.5.19|
Miki's Kernel & Shell (A personal diary on my academic and non-academic activities).
Field of Specialization
Marine Biochemistry, Comparative Immunology, Immunogenetics
My major research interest is structural and functional diversity of the complement components in bony fish. In contrast to mammalian complement system, bony fish have multiple copies of the genes encoding several complement components such as C3, C4, C5 and factor B/C2. Currently I am analyzing biological and evolutionary impact of the complement diversity on the innate immunity of the bony fish. Data obtained will be utilized for establishment of prevention of infectious diseases, which damage many kinds of cultured fish stock, and also for genotype-based selection of fish lineage with higher degree of host defense. Other research subject of my interest is phylogeny of the innate and adaptive immune system. Molecular cloning of innate immune components such as cytokines, complement components, and lectins will give some insights into genetic (genomic) mechanism that generated a number of immune-related genes in the lower vertebrates and invertebrates. Application of the finding obtained by the studies will be of importance to develop better means of disease prevention in fish and invertebrate aquaculture.
- Elucidation of structure, function and their diversity of the complement system in fish
keyword : fish, complement, structure, function, diversity
- Comparative Immunology of innate immune factors in aquatic vertebrates and invertebrates
keyword : fish, aquatic animals, invertebrates, comparative immunology, innate immunity
- Elucidation of crosstalk between innate and adaptive immune systems in fish
keyword : fish, innate immunity, adaptive immunity, acquired immunity, cellular immunity, interaction, crosstalk
|1.||Evolution of complement system in vertebrates in "Introduction to Complement", Ed. by Ooi H, Kinoshita T, and Matsushita M..|
|1.||Nakao M, Tsujikura M, Ichiki S, Vo TK, Somamoto T.,The complement system in teleost fish: Progress of post-homolog-hunting researches.,Epub,Dev Comp Immunol. ,2011.05.|
|2.||Phylogeny and evolution of activation pathways of the complement system.|
|3.||Nakao M, Kato-Unoki Y, Nakahara M, Mutsuro J and Somamoto T,Diversified components of bony fish complement system: More genes for robuster innate defense?,586: 121-138,Advances in Experimental Medicine and Biology,2006.03.|
|4.||Nakao M, Mutsuro J, Nakahara M, Kato Y, Yano T,Expansion of genes encoding complement components in bony fish: biological implications of the complement diversity.,27: 749-762.,Developmental and Comparative Immunology,2003.01.|
|1.||Ichiki S, Kato-Unoki Y, Somamoto T, Nakao M.,The binding spectra of carp C3 isotypes against natural targets independent of the binding
specificity of their thioester.,Developmental and Comparative Immunology,Vol.38,No.1,pp.10-16,2012.08.
|2.||Forlenza M, Nakao M, Wibowo I, Joerink M, Arts JA, Savelkoul HF, Wiegertjes GF,Nitric oxide hinders antibody clearance from the surface of Trypanoplasma borreli and increases susceptibility to complement-mediated lysis.,Molecular Immunology,Vol.46,No.16,pp.3188-3197,2009.10.|
|3.||VO Kha Tam, Masakazu TSUJIKURA, Tomonori SOMAMOTO, and Miki NAKAO,Expression responses of the complement components in zebrafish organs after stimulation with poly I:C, mimicry of viral infection,Journal of Faculty of Agriculture, Kyushu University,Vol.54,No.2,pp.389-395,2009.10.|
|4.||VO Kha Tam, Masakazu TSUJIKURA, Tomonori SOMAMOTO, and Miki NAKAO,Identification of cDNA sequences encoding the complement components of zebrafish (Danio rerio),Journal of Faculty of Agriculture, Kyushu University,Vol.54,No.2,pp.373-387,2009.10.|
|5.||Nevien K. Abdelkhalek, Asuka Komiya, Yoko Kato-Unoki, Tomonori Somamoto, Miki Nakao,Molecular evidence for the existence of two distinct IL-8 lineages of teleost CXC-chemokines,Fish & Shellfish Immunology,Vol.27,No.6,pp.763-767,2009.09.|
|6.||Dong-Ho Shin, Barbara M. Webb, Miki Nakao, Sylvia L. Smith,Characterization of shark complement factor I gene(s): Genomic analysis of a novel shark-speciﬁc sequence,Molecular Immunology,doi:10.1016/j.molimm.2009.04.002,2009.05.|
|7.||Nomiyama H, Hieshima K, Osada N, Kato-Unoki Y, Otsuka-Ono K, Takegawa S, Izawa T, Yoshizawa A, Kikuchi Y, Tanase S, Miura R, Kusada J, Nakao M, Yoshie O,Extensive Expansion and Diversification of the Chemokine Gene Family in Zebrafish: Identification of a Novel Chemokine Subfamily CX,BMC Genomics,9: 222.,2008.05.|
|8.||Nonaka S, Somamoto T, Kato-Unoki Y, Ototake M, Nakanishi T, Nakao M,Molecular cloning of CD4 from ginbuna crucian carp Carassius auratus langsdorfii ,Fisheries Science,74(2): 341-346,2008.04.|
|9.||Shin DH, Webb B, Nakao M, Smith SL,Molecular cloning, structural analysis and expression of complement component Bf/C2 genes in the nurse shark, Ginglymostoma cirratum
,Dev Comp Immunol,31(11):1168-1182,2007.04.
|10.||Somamoto T, Yoshiura Y, Sato A, Nakao M, Nakanishi T, Okamoto N, Ototake M.,Expression profiles of TCRbeta and CD8alpha mRNA correlate with virus-specific cell-mediated cytotoxic activity in ginbuna crucian carp.,Virology,348(2): 370-377,2006.05.|
|11.||Nakao M, Kajiya T, Sato Y, Somamoto T, Kato-Unoki Y, Matsushita M, Nakata M, Fujita T, Yano T,Lectin pathway of bony fish complement: Identification of two homologues of the mannose-binding lectin associated with MASP2 in the common carp (Cyprinus carpio),Journal of Immunology,177(8): 5471-5479,2006.10.|
|12.||Mutsuro J, Tanaka N, Kato Y, Dodds AW, Yano T, Nakao M,Two divergent isotypes of the fourth complement component from a bony fish, the common carp (Cyprinus carpio).,Journal of Immunology,Vol.175,No.7,175(7): 4508-4517,2005.10.|
|13.||Kato Y, Nakao M, Mutsuro J, Zarkadis IK, Yano T (2003),The complement component C5 of the common carp (Cyprinus carpio) : cDNA cloning of two distinct isotypes that differ in a functional site.,Immunogenetics,Vol.54,No.11,54: 807-815.,2003.01.|
|14.||Nakao M, Hisamatsu S, Nakahara M, Kato Y, Smith SL, Yano T,Molecular cloning of the complement regulatory factor I isotypes from the common carp (Cyprinus carpio).,Immunogenetics,Vol.54,No.11,54: 801-806.,2003.01.|
|15.||Fujiki K, Nakao M, Dixon B,Molecular cloning and characterization of a carp (Cyprinus carpio) cytokine-like cDNA that shares sequence similarity with IL-6 subfamily cytokines CNTF, OSM and LIF.,Developmental and Comparative Immunology,Vol.27,No.2,27: 127-136.,2003.01.|
|16.||Nakao M, Fujiki K, Kondo M, Yano T,Detection of complement receptors on head kidney phagocytes of the common carp (Cyprinus carpio).,Fisheries Science,Vol.69,No.5,69: 927-933.,2003.01.|
|17.||Nakao M, Uemura T, Yano T,Characterization of the soluble membrane attack complex (SMAC) of carp (Cyprinus carpio) complement.,Journal of Faculty of Agriculture, Kyushu University,Vol.48,No.1-2,48: 127-134.,2003.01.|
|18.||Ishikawa J, Imai E, Moritomo T, Nakao M, Yano T, Tomana M,Characterisation of a fourth immunoglobulin light chain isotype in the common carp.,Fish & Shellfish Immunology,Vol.16,No.3,16 (3), 369-379,2004.03.|
|19.||Kono T, Fujiki K, Nakao M, Yano T, Endo M, Sakai M,The immune responses of common carp, Cyprinus carpio L., injected with carp interleukin-1b gene.,Journal of Interferon and Cytokine Research,Vol.22,No.4,22: 413-419.,2002.01.|
|20.||Shimasaki Y, Oshima Y, Yokota Y, Kitano T, Nakao M, Kawabata S, Imada N and Honjo T,Purification and identification of a tributyltin-binding protein from serum of Japanese flounder, Paralichthys olivaceus.,Environmental Toxicology and Chemistry,Vol.21,No.6,21: 1229-1235.,2002.01.|
|21.||Nakao M, Matsumoto M, Nakazawa M, Fujiki K and Yano T,Diversity of complement factor B/C2 in the common carp (Cyprinus carpio): Three isotypes of B/C2-A expressed in different tissues.,Developmental and Comparative Immunology,Vol.26,No.6,26: 533-541.,2002.01.|
|22.||Tomana M, Ishikawa J, Imai E, Moritomo T, Nakao M, Yano T,Characterization of immunoglobulin light chain isotypes in the common carp.,Immunogenetics,Vol.54,No.2,54: 120-129.,2002.01.|
|23.||Nakao M, Osaka K, Kato Y, Fujiki K, Yano T,Molecular cloning of the complement C1r/C1s/MASP2-like serine proteases from the common carp (Cyprinus carpio).,Immunogenetics,Vol.52,No.3-4,52: 255-263.,2001.01.|
|24.||Fujiki K, Bayne CJ, Shin DH, Nakao M, Yano T,Molecular cloning of carp (Cyprinus carpio) C-type lectin and pentraxin by use of suppression subtractive hybridisation.,Fish & Shellfish Immunology,Vol.11,No.3,11:275-279.,2001.01.|
|25.||Bayne CJ, Gerwick L., Fujiki K., Nakao M, Yano T,Immune relevant genes identified in the liver of rainbow trout, Oncorhynchus mykiss, by means of suppression subtractive hybridization.,Developmental and Comparative Immunology,Vol.25,No.3,24: 205-217.,2001.01.|
|26.||Muturo J, Nakao M, Fujiki K, Yano T,Multiple forms of a2-macroglobulin from a bony fish, the common carp (Cyprinus carpio): Striking sequence diversity in functional sites.,Immunogenetics,Vol.51,No.10,51: 847-855.,2000.01.|
|1.||Carp thrombocytes are activated by other leukocytes to show phagocytosis..|
|2.||Phagocytic function of fish thrombocytes.|
|3.||Diversity in target-recognition of carp serum collectins related to the lectin pathway of the complement
Akito Ichiki, Haruka Tsukamoto, Tomonori Somamoto, and Miki Nakao.
|4.||Functional diversity of the complement component isotypes in bony fish innate immunity: a model study using the common carp.
Nakao, M., Ichiki, S., Mutsuro, J., Tsujikura, M., and Somamoto, T..
|5.||Functional differentiation of C7 isotypes in the common carp complement system.|
|6.||Recognition specificity of carp serum lectins associated with the complement system.|
|7.||Structural diversity and functional differentiation of the complement components in bony fish.
|8.||Functional differentiation of complement C3 isotypes in the teleost immune defense.
Satoko Ichiki, Yoko Kato-Unoki, Tomonori Somamoto, Miki Nakao.
|9.||Expression pattern of complement component C3 isotypes during early development of the common carp.
Vo Kha Tam, Chie Okura, Tomonori Somamoto, Miki Nakao.
|10.||Subcomponent structure of the first complement component (C1) in the common carp.
Takashi Ichii, Masakazu Tsujikura, Tomonori Somamoto, Yoko Kato-Unoki, Shin-ichi Kato, Michiyasu Yoshikuni, Miki Nakao.
|11.||Subcomponent structure of the first complement component (C1) in the common carp.|
|12.||Phagocytic ability of carpo thrombocytes.|
|13.||cDNA cloning and expression analysis of Fas ligand in ginbuna crucian carp.|
|14.||Cloning, purification and characterization of zymosan-binding proteins in Nile Tilapia (Oreochromis niloticus)
Soha Gomaa, Tomonori Somamoto and Miki Nakao.
|15.||Complement components constituting the classical activation pathway, an antibody-dependent activation cascade of the complement system..|
|16.||Diversity of carp serum lectins involved in the lectin pathway of the complement activation..|
|17.||Identification of a novel membrane-bound regulator of complement activation in carp and zebrafish.|
|18.||cDNA cloning of four lineages of MHC class I genes in a cell line from clonal ginbuna crucian carp, and their expression in the cell line infected with CHNV.|
|19.||Identification and expression analysis of two isotypes of carp complement component C7.|
|20.||Structural and functional diversity of the complement components in bony fish: implication for innate immune defense.|
|21.||Identification of a novel complement regulatory factor in bony fish.|
|22.||Structure and regulation of the isotypic genes of the complement factor B in the common carp.|
|23.||Structure and regulation of the isotypic genes of the complement factor B in the common carp.|
|24.||Antibacterial substances from bony fish: structure, function, and application.|
|25.||Identification and expression analysis of a novel interleukine 8 (IL-8)-like CXC chemokine in carp (Cyprinus carpio).|
|26.||Functional analysis of carp C3 isotypes using monoclonal antibodies.|
|27.||Identification of novel regulators of complement activation in fish.|
|28.||Functional analysis of carp C3 isoforms using monoclonal antibodies.|
|29.||Identification of Carp Complement Component C1.|
|30.||Expression analysis of complement component isotypes during early development of the common carp.|
|31.||cDNA cloning of MHC class I and MHC-related genes in a cell line from clonal ginbuna crucian carp.|
|32.||Expressed sequence tag analysis of kidney and spleen from ginbuna crucian carp,
Carassius auratus langsdorfii
|33.||Identification and expression analysis of zebrafish complement component genes.|
|34.||Recombinant carp complement component C4 and C5: Expression and functional analysis.|
|35.||Inference of bio-defensive functions of the complement system based on its gene expression pattern..|
|36.||Identification of the genes encoding complement components from the zebrafish genome database.|
|37.||EST Analysis of mRNAs Expressed in Gill and Intestine of Carp (Cyprinus carpio. L).|
|38.||Molecular cloning of a complement-regulatory factor from zebrafish.|
|39.||Molecular cloning of C3d, a fragment from complement component C3 with possible adjuvant activity, from marine cultured fish.|
|40.||Molecular cloning of C1q A, B and C chains from common carp.|
|41.||Molecular cloning of a novel complement-regulatory factor from the common carp.|
|42.||THE COMPLEMENT SYSTEM IN INVERTEBRATES AND LOWER VERTEBRATES.|
|43.||Molecular cloning and characterization of the complement C1q A, B, and C chains in common carp .|
|44.||MOLECULAR CLONING AND CHARACTERIZATION OF CD4 IN GINBUNA CRUCIAN CARP.|
|45.||DIVESIFICATION OF COMPLEMENT COMPONENT ISOTYPES IN THE COMMON CARP: EXPRESSION PATTERN AND SOME FUNCTIONAL ASPECTS.|
Undergraduage course: Fundamental Chemistry B, Introduction to Animal Production Science, Fish Immunology, Marine Biochemistry, Biochemical Experiment training, Marine Biochemical Experiment training.
Master course: Molecular and Cellular Biochemistry, Comparative Immunology
Master course: Molecular and Cellular Biochemistry, Comparative Immunology
Professional and Outreach Activities
Simple and rapid methods to monitor the degree of fish health are required to produce cultured fish. I have studied if serum complement acitivity is a good measure to estimate the level of bio-defense activity of cultured fish, aiming at development of a convenient kit for fish complement assay. Several natural materials have been tested for their possible immunostimulation.