Kyushu University Academic Staff Educational and Research Activities Database
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Ken-ichi Honjoh Last modified date:2019.04.19

Associate Professor / Division of Food Science and Biotechnology
Department of Bioscience and Biotechnology
Faculty of Agriculture


Graduate School
Undergraduate School


E-Mail
Homepage
http://www.agr.kyushu-u.ac.jp/lab/foodhygienicchemistry/eindex.html
Phone
092-802-4758
Fax
092-802-4757
Academic Degree
Ph.D of Agriculture
Country of degree conferring institution (Overseas)
No
Field of Specialization
Food Preservation
Outline Activities
Some plant species develop freezing tolerance when exposed to low, non-freezing temperatures. If freezing tolerance could be provided to cold-sensitive plants, the contribution to stable production of crops would be significant. In our laboratory, we found that hardened cells of Chlorella vulgaris C-27 survive slow freezing to -196°C. We are investigating the mechanism of the development of freezing tolerance of Chlorella. Furthermore, based on the research of Chlorella, enhancement of freezing tolerance of higher plants is also carried out.
Research
Research Interests
  • Development of a method for lowering contamination with Listeria to fresh produce during cultivation
    keyword : Listeria monocytogenes, fresh produce, decontamination
    2013.05.
  • Studies on new compatible solutes for freezing tolerance
    keyword : compatible solutes, freezing tolerance, taurine, tolehalose
    2009.04.
  • Studies on the mechanisms of the development of freezing tolerance of chlorella
    keyword : chlorella, freezing tolerance, hardening
    1991.04~2017.03Studies on the mechanism of the development of freezing tolerance of Chlorella.
  • Studies of enhancement of freezing tolerance of Saccharomyces cerevisiae
    keyword : freezing tolerance, Saccharomyces cerevisiae
    1996.04Enhancement of freezing tolerance of yeast.
  • Studies on the enhancement of freezing tolerance of higher plants
    keyword : freezing tolerance, Arabidopsis thaliana, lettuce, plants
    1994.04Enhancement of freezing tolerance of higher plants.
Academic Activities
Books
1. Takeshi Machida, Akiko Ishibashi, Eri Kato, Jun-ichi Sato, Shinji Kawasaki, Youichi Niimura, Ken-ichi Honjoh and Takahisa Miyamoto, Chloroplast NADPH-dependent thioredoxin reductase from Chlorella vulgaris functions as an antioxidant system with 2-Cys peroxiredoxin; The 5th International Joint Symposium between Japan and Korea. The Recent Status and Perspective of Food System, Agricultural Environment and Biology, 340-351, 2008.12.
Papers
1. Ken-ichi HONJOH, Hitomi OKANO, Aya KAWABATA, Masaru KUROKAWA, Taiki KIMURA, Takeshi MACHIDA, Yoshimitsu MASUDA and Takahisa MIYAMOTO, Freezing tolerance of Lactuca sativa and induction of CBF and GolS genes during cold treatment, Journal of Faculty of Agriculture, Kyushu University, 63, 2, 249-257, 2018.09.
2. Ken-ichi HONJOH, Yin LIN, Kiyomi JO, Yuri IWAIZAKO, Masayuki MAEDA, Nobuyuki KIJIMA, and Takahisa MIYAMOTO, Possible contamination routes of Listeria monocytogenes to leaf lettuce during cultivation, Food Science and Technology Research, 24, 5, 911-920, 2018.09.
3. Honjoh, K., Iwaizako, Y., Lin, Y., Kojima, N., Miyamoto, T., Possibilities of contamination of tomato fruits by Listeria monocytogenes during cultivation, Food Science and Technology Research, https://doi.org/10.3136/fstr.22.349, 22, 3, 349-357, 2016.05, [URL].
4. Approaches of Improvement of Freezing-Stress Tolerance of Yeast.
5. Honjoh, K., Mishima, T., Kido, N., Shimamoto, M., Miyamoto, T., Investigation of possible contamination routes of Salmonella via soils and the effects of mulch for avoiding its contamination to leafy lettuce plants during cultivation, Food Science and Technology Research, https://doi.org/10.3136/fstr.20.961, 20, 5, 961-969, 2014.09, [URL].
6. Machida, T., Ishibashi, A., Kirino, A., Sato, J., Kawasaki, S., Niimura, Y., Honjoh, K., Miyamoto, T., Chloroplast NADPH-dependent thioredoxin reductase from Chlorella vulgaris alleviates environmental stresses in yeast together with 2-Cys peroxiredoxin, PLoS ONE, 7, 9, e45988, 2012.09.
7. Machida, T., Honjoh, K., Aso, A., Yamamoto, M., Iio, M., Miyamoto, T., Trehalose 6-phosphate synthase and trehalose 6-phosphate phosphatase from Nicotiana tabacum function in trehalose biosynthesis and environmental stress tolerance of yeast, Journal of Faculty of Agriculture, Kyushu University, 55, 2, 261-268, 55, 2, 261-268, 2010.11.
8. Honjoh, K., Matsuura, K., Machida, T., Nishi, K., Nakao, M., Yano, T., Miyamoto, T., Iio, M., Enhancement of menadione stress tolerance in yeast by accumulation of hypotaurine and taurine: Co-expression of cDNA clones, from Cyprinus carpio, for cysteine dioxygenase and cysteine sulfinate decarboxylase in Saccharomyces cerevisiae, Amino Acids, 10.1007/s00726-009-0328-6., 38, 4, 1173-1183, 38(4): 1173-1183, 2010.04.
9. Machida, T., Ohashi, N., Mimura, A., Honjoh, K., Iio, M., Miyamoto, T., Chloroplastic glucose 6-phosphate dehydrogenase from Chlorella vulgaris alleviates freezing and menadione-indued oxidative stresses in Saccharomyces cerevisiae, Journal of the Faculty of Agriculture, Kyushu University , 55, 1, 29-38, 2010.03.
10. Machida, T., Honjoh, K., Shimizu, H., Yamamoto, M., Iio, M., Miyamoto, T., Expression of a gene encoding a functional glycosyl hydrolase, trehalase, from Nicotiana tabacum in Saccharomyces cerevisiae, Journal of the Faculty of Agriculture, Kyushu University , 54, 2, 297-304, 2009.11.
11. Machida, T., Kato, E., Ishibashi, A., Sato, J., Kawasaki, S., Niimura, Y., Honjoh, K., Miyamoto, T., Expression pattern of a chloroplast NADPH-dependent thioredoxin reductase in Chlorella vulgaris during hardening and its interaction with 2-Cys peroxiredoxin., Biosci. Biotechnol. Biochem., 73(3), 695-701, 2009.03.
12. Honjoh, K., Fujihara, K., Haraguchi, T., Ono, Y., Kobayashi, H., Hiwaki, H., Kamikado, H., Jang, S.S., Ryu, S., Miyamoto, T., Subtyping of Listeria monocytogenes based on nucleotide polymorphism in clpC, inlA, hlyA, and plcA genes and rapid identification of L. monocytogenes genetically similar to clinical isolates, Food Sci. Technol. Res., Vol. 14, No.6, 557-564, 2008.12.
13. Machida, T., Murase, H., Kato, E., Honjoh, K., Matsumoto, K., Miyamoto, T., Iio, M., Isolation of cDNAs for hardening-induced genes from Chlorella vulgaris by suppression subtractive hybridization., Plant Science, 175, 3, 238-246, 2008.09.
14. Machida, T., Kato, E., Ishibashi, A., Ohashi, N., Honjoh, K., Miyamoto, T., Molecular characterization of low-temperaure-inducible NTR-C in Chlorella vulgaris, Nucleic Acids Symposium Series, No. 51, 463-464, 2007.11.
15. Honjoh, K., Machida, T., Nishi, K., Matsuura, K., Soli, K.W., Sakai, T., Ishikawa, H., Matsumoto, K., Miyamoto, T., Iio, M., Improvement of freezing and oxidative stress tolerance in Saccharomyces cerevisiae by taurine, Food Sci. Technol. Res., 13,2, 145-154, 2007.06.
16. Honjoh,K., Machida, T., Hagisako, T., Suga, K., Yonekura, M., Shimizu, H., Ohashi, N., Miyamoto, T., Hatano, S., Iio, M., Molecular cloning and characterization of a cDNA for low-temperature inducible cytosolic glucose 6-phosphate dehydrogenase gene from Chlorella vulgaris and expression of the gene in Saccharomyces cerevisiae, Plant Science, 172, 649-658, 2007.03.
17. Honjoh, K., Mimura, A., Kuroiwa, E., Hagisako, T., Suga, K., Shimizu, H., Dubey, R.S., Miyamoto, T., Hatano, S., and Iio, M., Purification and characterization of two isoforms of glucose 6-phosphate dehydrogenase (G6PDH) from Chlorella vulgaris C-27, Biosci. Biotechnol. Biochem., 10.1271/bbb.67.1888, 67, 9, 1888-1896, 67(9): 1888-1896, 2003.09.
18. Honjoh, K., Suga, K., Shinohara, F., Maruyama, I., Miyamoto, T., Hatano, S., and Iio, M., Preparation of protoplasts from Chlorella vulgaris K-73122 and cell wall regeneration of protoplasts from C. vulgaris K-73122 and C-27, J. Fac. Agr., Kyushu Univ., 47, 2, 257-266, 47(2): 257-266, 2003.02.
19. Shimizu, H., Furuya, N., Suga, K., Honjoh, K., Miyamoto, T., Hatano, S., and Iio, M., Freezing tolerance of transgenic tobacco with increased content of unsaturated fatty acid by expressing the CvFad2 or CvFad3 gene, J. Fac. Agr., Kyushu Univ., 47, 2, 307-317, 47(2): 307-317, 2003.02.
20. Suga, K., Honjoh, K., Furuya, N., Shimizu, H., Nishi, K., Shinohara, F., Hirabaru, Y., Maruyama, I., Miyamoto, T., Hatano, S., and Iio, M.,, Two low-temperature-inducible Chlorella genes for D12 and w-3 fatty acid desaturase (FAD): Isolation of D12 and w-3 fad cDNA clones, expression of D12 fad in Saccharomyces cerevisiae , and expression of w-3 fad in Nicotiana tabacum, Biosci. Biotechnol. Biochem., 66, 6, 1314-1327, 66(6): 1314-1327, 2002.06.
21. Honjoh,K., Shimizu, H., Nagaishi, N., Matsumoto, H., Suga, K., Miyamoto, T., Iio, M., and Hatano, S., Improvement of freezing tolerance in transgenic tobacco leaves by expressing the hiC6 gene, Biosci. Biotechnol. Biochem., 10.1271/bbb.65.1796, 65, 8, 1796-1804, 65(8): 1796-1804, 2001.08.
22. Honjoh, K., Matsumoto, H., Shimizu, H., Ooyama, K., Tanaka, K., Oda, Y., Takata, R., Joh, T., Suga, K., Miyamoto, T., Iio, M. and Hatano, S., Cryoprotective activities of group3 late embryogenesis abundant proteins from Chlorella vulgaris C-27, Biosci. Biotechnol. Biochem., 10.1271/bbb.64.1656, 64, 8, 1656-1663, 64(8): 1656-1663, 2000.08.
23. K. Honjoh, Y. Oda, R. Takata, T. Miyamoto, and S. Hatano, Induction of the hiC6 gene, which encodes a homologue of a late embryogenesis abundant (LEA) protein, enhances freezing tolerance of yeast, Journal of Plant Physiology, Vol.155, No.4,5, 509-512, 1999.01.
24. Honjoh, K., Yoshimoto, M., Joh, T., Kajiwara, T., Miyamoto, T., Hatano, S., Isolation and characterization of hardening-induced proteins in Chlorella vulgaris C-27: Identification of late embryogenesis abundant proteins, Plant Cell Phyisiol., 36, 8, 1421-1430, 36(8), 1421-1430, 1995.12.
25. Joh, T., Honjoh, K., Yoshimoto, M., Funabashi, J., Miyamoto, T., Hatano, S., Molecular cloning and expression of hardening-induced genes in Chlorella vulgaris C-27: the most abundant clone encodes a late embryogenesis abundant protein, Plant Cell Physiol., 36, 1, 85-93, 36(1), 85-93, 1995.01.
26. Joh, T., Yoshimoto, M., Honjoh, K., Miyamoto, T., Hatano, S., Changes in translatable RNA population during hardening of Chlorella ellipsoidea C-27., J. Fac. Agr., Kyushu Univ., 37, 3-4, 257-263, 37(3,4), 257-263, 1993.02.
Presentations
1. Kimura, T., Kurokawa, M., Honjoh, K., Masuda, Y., Miyamoto T., Functional analysis of galactinol synthase gene from Lactica sativa in Escherichia coli, JSBBA West 1st Student Forum, 2018.11.
2. cDNA cloning of cold-induced galactinol synthase genes from lettuce.
3. Ken-ichi Honjoh, Tomoko Mishima, Nozomi Kido, Yuri Iwaizako, Ying Lin, Takahisa Miyamoto, Studies on contamination routes of Salmonella Enteritidis and Listeria monocytogenes in vegetables, 43rd Annual Meeting United States - Japan Cooperative Program in Natural Resources Food and Agricultural Panel, 2014.10.
4. Ken-ichi Honjoh, Hitomi Okano, Aya Kawabata, Ayako Shiraishi, Takahisa Miyamoto, Molecular response of Lactuca sativa L. to low temperature, 13TH ASEAN FOOD CONFERENCE 2013, 2013.09.
5. Functional analysis of new antioxidant enzymes of Chlorella for the development of freezing tolerance.
6. Expression of tobacco genes involved in trehalose biosynthesis in yeast..
7. Low-temperature-inducible NTR-C from Chlorella vulgaris acts as an electron donor to 2-Cys Prx and promotes peroxide detoxification.
8. Molecular cloning and characterization of cDNA for the low-temperature inducible NADPH-dependent thioredoxin reductase gene from Chlorella vulgaris.
9. Molecular cloning of cDNAs for two isoforms of glucose 6-phosphate dehydrogenase from Chlorella vulgaris and their expression patterns u
nder low temperature condition.
10. Isolation of cDNAs for hardening specific genes from Chlorella vulgaris by using suppression subtractive hybridization
Ken-ichi Honjoh, Takeshi Machida, Eri Kato, Hisako Murase, Masayoshi Iio, and Takahisa Miyamoto.
11. Cloning and expression of torehalose synthetase gene of tobacco.
12. Cloning of hardening inducible NADPH-thioredoxin reductase gene of Chlorella.
13. Cloning and expression behavour of g6pdh gene located in chloroplast of Chlorella.
14. Expression of Chlorella g6pdh gene in yeast and involvement of the gene in aquirement of freezing torelance..
15. FLUORESCENT MICROSCOPE WAS APPLIED FOR COUNTING VIABLE NUMBER IN MILK.
16. Genetic classification of Listeria monocytogenes based on DNA sequences of some specific genes.
17. Simultanious detection of 5 food poisoning bacteria in food.
18. Studies on taurine uptake using ABC transporter gene deletion sitain of yeast.
19. Screening of cold shock inducible genes of Chlorella by cDNA subtraction.
20. Cloning of genes involved in trehalose metabolism of tobacco plant.
21. Cloning and expression in yeast of taurine synthetase from carp.
Membership in Academic Society
  • Japanese Society for Safety Education
  • Japanese Society for Plant Cell and Molecular Biology
  • Japan Association of Food Preservation Science
  • Japan Society for Bioscience, Biotechnology, and Agrochemistry
  • The Japanese Society for Food Science and Technology
  • The Japanese Society of Plant Physiologists
  • Japanese Society of Food Microbiology