Kyushu University Academic Staff Educational and Research Activities Database
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MASAO SATO Last modified date:2019.06.24



Graduate School
Undergraduate School
Other Organization


Homepage
http://www.agr.kyushu-u.ac.jp/lab/nutrchem/
Home page of Lab. Nutrition chemistry .
Academic Degree
Ph.D
Country of degree conferring institution (Overseas)
No
Field of Specialization
Nutrition Chemistery
Total Priod of education and research career in the foreign country
01years02months
Outline Activities
<Research>
1.Control of lipid metabolism with food ingredients
2.Effects of food ingredients on development of arteriosclerosis
3.Effects of exogenous and endogenous oxycholesterols on lipid metabolism
4.Relationship analysis between metabolic syndrome and serum fatty acid compositions in human
5.Epigenetic regulation of gene expression by dietary intervention
6.Identification of responsible genes for hypercholesterolemia of disease model animals
7.Effects of food ingredients on development of obesity
Research
Research Interests
  • Analysis of relation between serum fatty acid composition and disease
    keyword : serum fatty acid composition
    2005.04.
  • Development of rice oil source from breakthrough rice strain
    keyword : rice, rice bran oil
    2009.09.
  • Metabolism and absorption of nutrients
    keyword : lymph duct, lipids, Intestinal mucosa
    1989.04.
  • Development of anti-metabolic syndrome food
    keyword : metabolic syndrome, lipid metabolism
    2005.04.
  • Investigation of metabolic memory via DNA methylation by lipid nutrients
    keyword : DNA Methylation, Metabolic memory
    2003.04.
  • 動脈硬化症発症の原因となるリポタンパク質profileを変化させる食事を探索している。
    keyword : Atherosclerosis,HDL,LDL
    1984.04~1991.03.
  • Mechanism of diet induced hypercholesterlemia.
    keyword : ExHC rat, Linkage mapping
    1984.04.
  • Effects of dietary and endogenous oxycholesterols on various deseases
    keyword : oxycholesterol
    2004.04.
  • Effects of diets on lipid metabolism and atherosclerosis
    keyword : diet, lipid metあbolism
    1997.04.
Academic Activities
Reports
1. Key Roles of Cholesterol in vivo.
2. Oxycholesterol In Vivo and Physiologycal Function - Molecular Itemized Discussion -.
3. Anti-metabolic syndrome effect by probiotics.
4. Anti-metabolic syndrome effect by probiotics.
5. Absorption of oxysterols and the related diseases.
6. Absorption and metabolism of oxidaized sterols and their function .
7. Current opinion in nutrigenomics and life-style related disease.
Papers
1. Shirouchi B, Furukawa Y, Nakamura Y, Kawauchi A, Imaizumi K, Oku H, Sato M., Inhibition of Niemann-Pick C1-Like 1 by Ezetimibe Reduces Dietary 5β,6β-Epoxycholesterol Absorption in Rats., Cardiovasc Drugs Ther, 10.1007/s10557-019-06854-4, 2019.05.
2. Niibo, M., Shirouchi, B., Umegatani, M., Morita, Y., Ogawa, A., Sakai, F., Kadooka, Y., Sato, M, Probiotic Lactobacillus gasseri SBT2055 improves insulin secretion in a diabetic rat model, Journal of Dairy Science, 10.3168/jds.2018-15203, 102, 2, 997-1006, 2019.02.
3. Takeyama, A., Nagata, Y., Shirouchi, B., Nonaka, C., Aoki, H., Haraguchi, T., Sato, M., Tamaya, K., Yamamoto, H., Tanaka, K., Dietary Sparassis crispa reduces body fat mass and hepatic lipid levels by enhancing energy expenditure and suppressing lipogenesis in rats, Journal of Oleo Science, 10.5650/jos.ess18043, 67, 9, 1137-1147, 2018.09, Accumulation of abdominal fat triggers metabolic syndrome, which is a cluster of metabolic abnormalities, such as dyslipidemia, glucose intolerance, insulin resistance or hyperinsulinemia, and hypertension, that leads to the development of diabetes and cardiovascular disease. Mushrooms have been used as a foodstuff and folk medicine worldwide. Among these mushrooms, Sparassis crispa (SC) is a relatively newly cultivated edible and medicinal mushroom, which has been reported to have anti-diabetic and anti-hypertensive properties. However, little is known about the anti-obesity and anti-hyperlipidemic properties of SC. In the present study, we investigated the effects of dietary SC on lipid metabolism and energy expenditure in Sprague-Dawley rats with diet-induced obesity and diabetes, and conducted respiratory gas analysis to determine how energy metabolism is altered by SC. After feeding periods of 3 and 7 weeks, dietary SC had significantly reduced hepatic triacylglycerol and cholesterol contents in a dose-dependent manner. These changes were attributable to suppression of fatty acid and cholesterol synthesis in the liver and increased insulin sensitivity in the body. In addition, after a feeding period of 6 weeks, dietary SC significantly increased energy expenditure through carbohydrate oxidation, reducing abdominal fat mass after 7 weeks. In conclusion, our results indicate that in addition to the previously reported anti-diabetic and anti-hypertensive activities, dietary SC exhibits anti-obesity and anti-hyperlipidemic activities that help protect against metabolic syndrome..
4. Shirouchi, B., Yamanaka, R., Tanaka, S., Kawatou, F., Hayashi, T., Takeyama, A., Nakao, A., Goromaru, R., Iwamoto, M., Sato, M., Quantities of phospholipid molecular classes in japanese meals and prediction of their sources by multiple regression analysis, Journal of Nutritional Science and Vitaminology, 10.3177/jnsv.64.215, 64, 3, 215-221, 2018.05, Dietary intake of total phospholipids (PLs) accounts for approximately 10% of total dietary lipids. Each PL molecular class has various beneficial effects on health. However, limited information is available regarding the intake of phosphatidylcholine (PC), phos-phatidylethanolamine (PE), phosphatidylinositol (PI), phosphatidylserine (PS), lysophos-phatidylcholine (LPC) and sphingomyelin (SM) among Japanese people, and the relevant food sources. In this study, we quantified the contents of PC, PE, PI, PS, LPC, and SM in 120 meal samples served in a Japanese company’s dormitory and cafeteria. Additionally, we measured the weight of each food group and estimated the contents of nutrients in these meals. Furthermore, we conducted a stepwise multiple regression analysis to identify pre-dictors (food groups) of each PL class intake. The contents of total PL, PC, PE, PI+PS, LPC, and SM (mean value) were 4.44, 2.17, 0.632, 0.123, 0.313, and 0.127 g/d, respectively. These values were considered as daily PL intake in accordance with data (three macronu-trients, vitamins, and minerals) from our study and the National Health and Nutrition Survey (NHNS) Japan, 2015. The content of eggs, meat, fish and shellfish, milk, pulses, fruits, mushrooms, cereals, and fats and oils in the meals predicted the PL and PC contents. The content of eggs, pulses, and mushrooms in the meals predicted the PE contents. Our results determined the daily intake of PL molecular classes among Japanese people and the food sources of PC and PE, and suggested that multiple regression analysis is useful for the prediction of food sources of bioactive components.
5. Kimura Y, Yasuda K, Kurotani K, Akter S, Kashino I, Hayabuchi H, Sato M, Mizoue T, Circulating ferritin concentrations are differentially associated with serum adipokine concentrations in Japanese men and premenopausal women., Eur J Nutr,, 10.1007/s00394-016-1285-y, 56, 8, 2497-2505, 2017.12, PURPOSE:
Increased iron storage, as measured by circulating ferritin, has been linked to an increased risk of various diseases including diabetes. We examined the association of circulating ferritin with serum adiponectin, leptin, resistin, plasminogen activator inhibitor-1 (PAI-1), and visfatin levels.
METHODS:
We conducted a cross-sectional study among 429 Japanese employees (284 men and 145 premenopausal women, mean age: 42.5 ± 10.5 years). Serum adipokines were measured using Luminex suspension bead-based multiplexed array, and serum ferritin was determined using a chemiluminescence immunoassay. Multivariable regression analysis was performed to calculate mean concentrations of adipokine according to the tertile of ferritin concentrations with adjustment for potential confounders.
RESULTS:
Leptin and visfatin concentrations increased with increasing ferritin concentrations in men after multivariable adjustment of physical activity, smoking, alcohol use, and body mass index (P for trend = 0.02 and 0.01 for leptin and visfatin, respectively). Serum ferritin concentrations were inversely and significantly associated with adiponectin in women (P for trend = 0.01). Resistin and PAI-1 were not appreciably associated with ferritin concentration.
CONCLUSIONS:
Increased iron storage may be associated with higher circulating concentrations of leptin and visfatin in men and with lower concentrations of adiponectin in women..
6. Matsuoka R, Shirouchi B, Umegatani M, Fukuda M, Muto A, Masuda Y, Kunou M, Sato M, Dietary egg-white protein increases body protein mass and reduces body fat mass through an acceleration of hepatic β-oxidation in rats., Br J Nutr., 10.1017/S0007114517002306, 118, 6, 423-430, 2017.09, Egg-white protein (EWP) is known to reduce lymphatic TAG transport in rats. In this study, we investigated the effects of dietary EWP on body fat mass. Male rats, 4 weeks old, were fed diets containing either 20 % EWP or casein for 28 d. Carcass protein levels and gastrocnemius leg muscle weights in the EWP group were significantly higher than those in the casein group. In addition, carcass TAG levels and abdominal fat weights in the EWP group were significantly lower than those in the casein group; adipocyte size in abdominal fat in the EWP group was smaller than that in the casein group. To identify the involvement of dietary fat levels in the rats, one of two fat levels (5 or 10 %) was added to their diet along with the different protein sources (EWP and casein). Abdominal fat weight and serum and hepatic TAG levels were significantly lower in the EWP group than in the casein group. Moreover, significantly higher values of enzymatic activity related to β-oxidation in the liver were observed in the EWP group compared with the casein group. Finally, abdominal fat weight reduction in the EWP group with the 10 % fat diet was lower than that in the EWP group with the 5 % fat diet. In conclusion, our results indicate that, in addition to the inhibition of dietary TAG absorption reported previously, dietary EWP reduces body fat mass in rats through an increase of body protein mass and the acceleration of β-oxidation in the liver..
7. Akter, S., Email Author, Kurotani, K., Sato, M., Hayashi, T., Kuwahara, K., Matsushita, Y., Nakagawa, T., Konishi, M., Honda, T., Yamamoto, S., Hayashi, T., Noda, M., Mizoue, T., High serum phospholipid dihomo-γ-linoleic acid concentration and low Δ5-desaturase activity are associated with increased risk of type 2 diabetes among Japanese adults in the hitachi health study, J Nutri., 10.3945/jn.117.248997, 147, 8, 1558-1566, 2017.08, Background: The association between the circulating fatty acid (FA) composition and type 2 diabetes (T2D) has been reported in Western populations, but evidence is scarce among Asian populations, including Japanese, who consume large amounts of fish. Objective: The objective of the present study was to prospectively examine the association between circulating concentrations of individual FAs and T2D incidence among Japanese adults. Methods: We conducted a nested case-control study in a cohort of 4754 employees, aged 34-69 y, who attended a comprehensive health checkup in 2008-2009 and donated blood samples for the Hitachi Health Study. During 5 y of follow-up, diabetes was identified on the basis of plasma glucose, glycated hemoglobin, and self-report. Two controls matched to each case by sex, age, and date of checkup were randomly chosen by using density sampling, resulting in 336 cases and 678 controls with FA measurements. GC was used to measure the FA composition in serum phospholipids. Cox proportional hazards regression was used to estimate the HRs and 95% CIs after adjusting for potential confounders. We examined the association of T2D risk with 25 different individual and combinations of FAs. Results: T2D risk was positively associated with serum dihomo-γ-linoleic acid concentration (highest compared with the lowest quartile-HR: 1.49; 95% CI: 1.04, 2.11; P-trend = 0.02) and inversely associated with Δ5-desaturase activity (highest compared with the lowest quartile-HR: 0.72; 95% CI: 0.52, 0.99; P-trend = 0.02), independent of body mass index (BMI). There were also inverse associations between T2D risk with serum total n-6 (ω-6) polyunsaturated fatty acids (PUFAs), linoleic acid, and cis-vaccenic acid, but these were attenuated and became nonsignificant after adjustment for BMI. Serum n-3 (ω-3) PUFAs and saturated fatty acids (SFAs) were not associated with T2D risk. Conclusions: T2D risk was associated with circulating concentrations of the n-6 PUFA dihomo-γ-linoleic acid and Δ5-desaturase activity but not with n-3 PUFA or SFA concentrations in Japanese adults..
8. Hiraki E, Furuta S, Kuwahara R, Takemoto N, Nagata T, Akasaka T, Shirouchi B, Sato M, Ohnuki K, Shimizu K., Anti-obesity activity of Yamabushitake (Hericium erinaceus) powder in ovariectomized mice, and its potentially active compounds., J Nat Med, 10.1007/s11418-017-1075-8, 71, 3, 482-491, 2017.07, Hericium erinaceus (H. erinaceus) improves the symptoms of menopause. In this study, using ovariectomized mice as a model of menopause, we investigated the anti-obesity effect of this mushroom in menopause. Mice fed diets containing H. erinaceus powder showed significant decreases in the amounts of fat tissue, plasma levels of total cholesterol, and leptin. To determine the mechanism, groups of mice were respectively fed a diet containing H. erinaceus powder, a diet containing ethanol extract of H. erinaceus, and a diet containing a residue of the extract. As a result, H. erinaceus powder was found to increase fecal lipid levels in excreted matter. Further in vitro investigation showed that ethanol extract inhibited the activity of lipase, and four lipase-inhibitory compounds were isolated from the extract: hericenone C, hericenone D, hericenone F, and hericenone G. In short, we suggest that H. erinaceus has an anti-obesity effect during menopause because it decreases the ability to absorb lipids..
9. Bungo Shirouchi, Kentaro Kashima, Yasutaka Horiuchi, Yuki Nakamura, Yumiko Fujimoto, Li-Tao Tong, MASAO SATO, 27-Hydroxycholesterol suppresses lipid accumulation by down-regulating lipogenic and adipogenic gene expression in 3T3-L1 cells, Cytotechnology, 10.1007/s10616-016-9962-5, 69, 3, 485-492, 2017.06.
10. Kurotani K, Sato M, Yasuda K, Kashima K, Tanaka S, Hayashi T, Shirouchi B, Akter S, Kashino I, Hayabuchi H, Mizoue T., Even- and odd-chain saturated fatty acids in serum phospholipids are differentially associated with adipokines., PLoS One, 10.1371/journal.pone.0178192, 12, 5, e0178192, 2017.05, BACKGROUND:
Saturated fatty acids are generally thought to have detrimental effects on health. However, a recent study showed that even- and odd-chain saturated fatty acids had opposite associations with type 2 diabetes. Limited studies of Western populations examined the associations of circulating saturated fatty acids with adipokines, an important role in glucose metabolism.
OBJECTIVE:
We examined the associations of saturated fatty acids in serum phospholipids with circulating levels of adipokines among a Japanese population.
DESIGN:
A cross-sectional study was conducted among 484 Japanese employees (284 men and 200 women) aged 20-65 years. The serum fatty acid composition in the phospholipid fraction was measured by gas-chromatography. Serum leptin, adiponectin, plasminogen activator inhibitor-1 (PAI-1), resistin, and visfatin were measured using a Luminex suspension bead-based multiplexed array. Multiple linear regression analysis was performed to assess the association between saturated fatty acids and adipokines, with adjustment for potential confounding variables.
RESULTS:
Even- and odd-chain saturated fatty acids were differentially associated with adipokines. Higher levels of even-chain saturated fatty acids (14:0 myristic, 16:0 palmitic, and 18:0 stearic acids) were associated with higher levels of resistin (P for trend = 0.048) and lower levels of adiponectin (P for trend = 0.003). By contrast, odd-chain saturated fatty acids (15:0 pentadecanoic and 17:0 heptadecanoic acids) showed inverse associations with leptin and PAI-1 (P for trend = 0.048 and 0.02, respectively). Visfatin was positively associated with both even- and odd-chain saturated fatty acids.
CONCLUSIONS:
The results suggest that even- and odd-chain saturated fatty acids are differentially associated with adipokine profile..
11. Michio Shimabukuro, Chinami Okawa, Shuhei Yanagi, Etsuko Uematsu, Noriko Sugasawa, Hirotsugu Kurobe, Yoichiro Hirata, Joo-ri Kim-Kaneyama, Xiao-Feng Lei, Shoichiro Takao, Yasutake Tanaka, Daiju Fukuda, Shusuke Yagi, Takeshi Soeki, Tetsuya Kitagawa, Hiroaki Masuzaki, MASAO SATO, Masataka Sata, The pathophysiological role of oxidized cholesterols in epicardial fat accumulation and cardiac dysfunction: a study in swine fed a high caloric diet with an inhibitor of intestinal cholesterol absorption, ezetimibe, Journal of Nutritional Biochemistry, 10.1016/j.jnutbio.2016.05.010, 35, 1, 66-73, 2016.09, Oxidized cholesterols (oxycholesterols) in food have been recognized as strong atherogenic components, but their tissue distributions and roles in cardiovascular diseases remain unclear. To investigate whether accumulation of oxycholesterols is linked to cardiac morphology and function, and whether reduction of oxycholesterols can improve cardiac performance, domestic male swine were randomized to a control diet (C), high caloric diet (HCD) or HCD + Ezetimibe, an inhibitor of intestinal cholesterol absorption, group (HCD + E) and evaluated for: (1) distribution of oxycholesterol components in serum and tissues, (2) levels of oxycholesterol-related enzymes, (3) paracardial and epicardial coronary fat thickness, and (4) cardiac performance. Ezetimibe treatment for 8 weeks attenuated increases in oxycholesterols in the HCD group almost completely in liver, but reduced only levels of 4β-hydroxycholesterol in left ventricular (LV) myocardium. Ezetimibe treatment altered the expression of genes for cholesterol and fatty acid metabolism and decreased the expression of CYP3A46, which catabolizes cholesterol to 4β-hydroxycholesterol, strongly in liver. An increase in epicardial fat thickness and impaired cardiac performance in the HCD group were improved by ezetimibe treatment, and the improvement was closely related to the reduction in levels of 4β-hydroxycholesterol in LV myocardium. In conclusion, an increase in oxycholesterols in the HCD group was closely related to cardiac hypertrophy and dysfunction, as well as an increase in epicardial fat thickness. Ezetimibe may directly reduce oxycholesterol in liver and LV myocardium, and improve cardiac morphology and function..
12. Kazuo ERAMI, Yasutake TANAKA, Sayaka KAWAMURA, Motonori MIYAGO, Ai SAWAZAKI, katsumi imaizumi, MASAO SATO, Dietary Egg Yolk Supplementation Improves Low-Protein-Diet-Induced Fatty Liver in Rats, Journal of Nutritional Science and Vitaminology, 10.3177/jnsv.62.240, 62, 4, 240-248, 2016.08, [URL], Egg yolk is an important source of nutrients and contains different bioactive substances. In the present study, we studied the benefits of egg yolk in preventing low-protein-diet-induced fatty liver in rats. Rats were fed the following diets, which were based on the AIN-76 formula, for 2 wk: an adequate-protein diet containing 20% casein (C), a low-protein diet containing 5% casein (LP-C), a low-protein diet supplemented with 12.5% egg yolk (LP-EY), and a low-protein diet supplemented with 4.1% egg yolk oil (LP-EYO). The low-protein diets were adjusted to contain 4.13% protein and 4.7% lipids. The LP-C diet resulted in a greater increase in the liver trigriceride (TG) and the vacuolation and a greater decrease in the serum TG and free fatty acid (FFA) than did the C diet. These deviations in the serum and liver TG, serum FFA levels and the liver histopathology were corrected in rats fed the LP-EY diet but not in those fed the LP-EYO diet. Compared to rats fed the LP-C diet, although the activities of lipogenesis-related enzymes (fatty acid synthase, glucose-6-phosphate dehydrogenase, and malic enzyme) decreased in rats fed both of the LP-EY and LP-EYO diets, the level of the microsomal TG transfer protein (MTP) increased only in rats fed the LP-EY diet. Collectively, these results suggest that dietary egg yolk supplementation decreases the LP diet-induced accumulation of TG in the liver by increasing transport of TG in the liver, and egg yolk oil alone is not sufficient enough to bring about these benefits..
13. Bungo Shirouchi, Kouji Nagao, Minami Umegatani, Aya Shiraishi, Yukiko Morita, Shunichi Kai, Teruo Yanagita, Akihiro Ogawa, Yukio Kadooka, MASAO SATO,  Probiotic Lactobacillus gasseri SBT2055 improves glucose tolerance and reduces body weight gain in rats by stimulating energy expenditure., British Journal of Nutrition, 116, 451-458, 2016.05.
14. Ikuko Kashino, Akiko Nanri, Kayo Kurotani, Shamima Akter, Kazuki Yasuda, MASAO SATO, Hitomi Hayabuchi, Tetsuya Mizoue, Association of dietary patterns with serum adipokines among Japanese: a cross-sectional study
, Nutrition Journal, 10.1186/s12937-015-0046-8., 14, 58, 2015.08.
15. Ngoc Minh Phama, Akiko Nanri, Siyan Yi, Kayo Kurotani,, Shamima Akter, Leng Huat Foo, Nobuo Nishi, MASAO SATO, Hitomi Hayabuchi, Tetsuya Mizoue, Habitual consumption of coffee and green tea in relation to serum adipokines: a cross-sectional study, European Journal of Nutrition , 10.1007/s00394-014-0701-4, 54, 2, 205-214, 2015.03, PURPOSE:Coffee and green tea consumption may be associated with circulating adipokines, but data are inconsistent, scarce or lacking. We examined the association of coffee and green tea consumption with serum adiponectin, leptin, visfatin, resistin and plasminogen activator inhibitor-1 (PAI-1) among a Japanese working population.
METHODS:
The authors analyzed data (n = 509) from a cross-sectional survey among Japanese workers aged 20-68 years. Serum adipokines were measured using a Luminex suspension bead-based multiplexed array. Coffee and green tea consumption was assessed using a validated diet history questionnaire, and caffeine consumption from these beverages was estimated. Multiple regression analysis was performed with adjustment for potential confounding variables.
RESULTS:
Coffee consumption was significantly, inversely associated with leptin and PAI-1 (P for trend = 0.007 and 0.02, respectively); compared with subjects consuming <1 cup per day, those consuming ≥4 cups per day had 13 and 10 % lower means of leptin and PAI-1, respectively. Similar associations were observed for caffeine consumption (P for trend = 0.02 for both leptin and PAI-1). Additionally, we noted a significant positive association between coffee consumption and adiponectin in men (P for trend = 0.046), but not in women (P for trend = 0.43, P for interaction = 0.11). Moreover, there was a positive association between coffee consumption and resistin in current male smokers (P for trend = 0.01), but not in male non-smokers (P for trend = 0.35, P for interaction = 0.11). Green tea consumption was not associated with any adipokine.
CONCLUSIONS:
Higher consumption of coffee and caffeine but not green tea was associated with lower serum levels of leptin and PAI-1 in Japanese adults.
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16. Ryousuke Matsuoka, Bungo Shirouchi, Masao Sato, Sayaka Kawamura, Sanae Baba, Sawako Shiratake, Kazuko Nagata, Katsumi Imaizumi, Dietary Egg White Protein Inhibits Lymphatic Lipid Transport in Thoracic Lymph Duct-Cannulated Rats, Journal of Agricultural and Food Chemistry, 10.1021/jf502741b, 62, 44, 10694-10700, 2014.11, Dietary egg white protein (EWP) decreases serum cholesterol levels. We previously showed that EWP decreased cholesterol absorption in the intestine. Rats subjected to permanent lymph duct cannulation were used to investigate the effects of dietary EWP on lipid transport. They were fed diets with 20% EWP and casein, and their lymph was collected to quantify lymphatic lipid levels. Dietary EWP decreased lymphatic cholesterol transport compared with casein. It was previously shown that EWP excluded cholesterol from bile acid micelles. Therefore, pepsin-hydrolyzed EWP and casein were prepared. EWP was not completely digested. Ovalbumin, which is the most abundant protein in EWP, showed resistance to digestion by pepsin. This study investigated the effects of EWP pepsin hydrolysate (EWP-ph) on cholesterol micellar solubility, cholesterol transfer from the micellar to the oil phase, water-holding capacity (WHC), settling volume in water (SV), and relative viscosity and compared them with the effects of casein pepsin hydrolysate (C-ph). EWP-ph significantly decreased the micellar solubility and transfer rate and increased the WHC, SV, and relative viscosity compared with C-ph. Moreover, the pepsin hydrolysate of ovalbumin, a major protein in EWP, played a role in decreasing cholesterol micellar solubility, leading to the inhibition of cholesterol absorption. In conclusion, dietary EWP decreased cholesterol intestinal absorption by exerting combined effects of these physicochemical properties in the gut..
17. Ai Hori, Hiroshi Kasai, Kazuaki Kawai, Akiko Nanri, MASAO SATO, Masanori Ohta, Tetsuya Mizoue, COFEE INTAKE IS ASSOCIATED WITH REDUCING OXIDATIVE DNA DAMAGE AND DECREASING BODY IRON STORAGE IN HEALTHY WOMEN, Nutrition and Cancer , 10.1080/01635581.2014.932398., 66, 6, 964-969, 2014.06, Habitual coffee drinking has been linked to a lower risk for some forms of cancer, but the mechanism remains elusive. Coffee may decrease oxidative DNA damage, an important pathway to carcinogenesis. We examined the association between coffee consumption and urinary 8-hydroxydeoxyguanosine (8-OHdG) concentrations, a biomarker of systemic oxidative DNA damage and repair, in 507 healthy subjects (298 men and 209 women aged 21-67 years) while adjusting for age, sex, smoking status, body mass index, job type, and fasting blood glucose in multivariable regression models. The association with green tea consumption was also assessed. Urinary 8-OHdG concentrations tended to decrease with coffee consumption (Trend P=0.068) only in women, with participants drinking 2-3 cups of coffee per day showing the lowest mean of urinary 8-OHdG concentrations. This association diminished after adjustment for serum ferritin concentrations, a marker of body iron storage (Trend P=0.87). Green tea consumption was not significantly associated with urinary 8-OHdG concentrations. Coffee drinking may be associated with decreased systemic oxidative DNA damage through decreasing body iron storage in women..
18. Hiroyuki Kikuchi, Akiko Nanri, Ai Hori, MASAO SATO, Kazuaki Kawai, Hiroshi Kasai, Tetsuya Mizoue, Lower serum levels of total cholesterol are associated with higher urinary levels of 8-hydroxydeoxyguanosine, Nutirition and Metabolism, 10.1186/10.1186/1743-7075-10-59, 10, Art. NO. 59, 2014.03, [URL], Background
Lower serum total (TC), high-density lipoprotein (HDL-C) and low-density lipoprotein cholesterols (LDL-C) have been linked to an increased risk of cancer in various sites, but its underlying mechanism remains unclear. In an attempt to clarify the association between cholesterol levels and oxidative DNA damage, we investigated the relationship between serum cholesterol and urinary 8-hydroxydeoxyguanosine levels in a Japanese working population.
Methods
The study subjects were 294 men and 209 women aged 21-66 years in two Japanese municipal offices. Urinary 8-hydroxydeoxyguanosine (8-OHdG) was measured using an automated high-pressure liquid chromatography. Linear regression analysis was used to examine the associations of urinary 8-OHdG with TC, HDL-C and LDL-C levels with adjustment for sex, age, smoking and body mass index. Subgroup analyses were conducted by smoking status in men and age in women. Analysis of covariance was employed to estimate adjusted means of urinary 8-OHdG across TC category.
Results
After multivariate adjustment, urinary 8-OHdG levels were inversely associated with serum TC levels (β = −0.0015, p < 0.05) and LDL-C levels (β = −0.0012, p = 0.07). The inverse association with TC was apparent among smoking men (β = −0.0017, p < 0.05) and among women aged less than 48 years (β = −0.0040, p < 0.01). 8-OHdG decreased as TC increased (up to 219 mg/dL); subjects with TC levels of <160 mg/dL had a 17.4% higher adjusted mean of 8-OHdG than did those with TC levels of 200–219 mg/dL.
Conclusion
Results suggest that circulating low TC levels are associated with higher oxidative DNA damage.
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19. Ngoc Minh Pham, Akiko Nanri, Takeshi Kochi, Keisuke Kuwahara, Hiroko Tsuruoka, Kayo Kurotani, Shamima Akter, Isamu Kabe, MASAO SATO, Hitomi Hayabuchi, Tetsuya Mizoue, Coffee and green tea consumption is associated with insulin resistance in Japanese adults, Metabolism - Clinical and Experimental, 10.1016/j.metabol.2013.11.008, 63, 3, 400-408, 2014.03, Objective. Higher coffee and green tea consumption has been suggested to decrease risk of type 2 diabetes, but their roles in insulin resistance (IR) and insulin secretion remain unclear. This study examined the association between habitual consumption of these beverages with markers of glucose metabolism in a Japanese working population.
Materials/Methods. Participants were 1,440 Japanese employees (1,151 men and 289 women) aged 18-69 years. Consumption of coffee and green tea was ascertained via a validated brief diet history questionnaire. Multilevel linear regression was used to estimate means and 95% confidence intervals of fasting insulin, fasting plasma glucose, homeostatic model assessment of IR (HOMA-IR), homeostatic model assessment of β-cell function (HOMA-β) and glycated hemoglobin (HbA1c) with adjustment for potential confounding variables. Stratified analyses by smoking and body mass index (BMI) were also performed.
Results. Coffee consumption was significantly, inversely associated with HOMA-IR (P for trend = .03), and the association appeared to be confined to overweight subjects (BMI ≥25kg/m2 ) (P for trend = .01, P for interaction = .08). Contrarily, there was a significant, positive association between green tea consumption and HOMA-IR (P for trend = .015), though dose response relationship among daily consumers of green tea was not observed. Neither coffee nor green tea consumption was associated with HOMA-β and HbA1c.
Conclusions. Our findings indicate that coffee consumption may be associated with decreased IR, but not with insulin secretion. The positive association between green tea consumption and IR warrants further investigation..
20. Kayo Kurotani, MASAO SATO, Yuko Ejima, Kentaro Kashima, Akiko Nanri, Ngoc Minh Pham, Keisuke Kuwahara, Tetsuya Mizoue, Serum alpha-linolenic and linoleic acids are inversely associated with depressive symptoms in adults, e-SPEN Journal, 10.1016/j.clnme.2013.12.003, 9, 1, e7-e12, 2014.02, Background & Aims: Fatty acid composition in diet and serum has been linked to depression, but the evidence on this issue is limited among Japanese, who consume large amounts of fish rich in n-3 polyunsaturated fatty acids. We examined the association between fatty acid composition in serum and depressive symptoms in Japanese men and women.
Methods: The subjects were 496 participants aged 21-67 years in a cross-sectional study. Depressive symptoms were assessed using the Center for Epidemiologic Studies Depression (CES-D) scale. Free, cholesterol ester, and phospholipid fatty acids in serum were measured by gas-liquid chromatography. Multiple logistic regression was used to assess the association between serum fatty acid and depressive symptoms.
Results: A higher free alpha-linolenic acid level was marginally significantly associated with a decreased prevalence of depressive symptoms (CES-D≥16) (P for trend = 0.07). When a cut-off of CES-D≥19 was used, the association was strengthened; the multivariable-adjusted odds ratios (95% confidence interval) of depressive symptoms for the lowest through highest tertile of alpha-linolenic acid levels were 1.00 (reference), 0.49 (0.29-0.84), and 0.47 (0.26-0.83), respectively (P for trend = 0.007). A higher n-6 PUFA in cholesterol esters and free linoleic acid were also significantly associated with a decreased prevalence of depressive symptoms (CES-D≥19) (P for trend = 0.03 and 0.048, respectively). The other polyunsaturated fatty acids including marine-derived n-3 PUFA were not associated with depressive symptoms.
Conclusions: Fatty acid composition with high levels of serum alpha-linolenic and linoleic acids might be protectively associated with depressive symptoms in Japanese adults.
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21. Yasutake Tanaka, Koji Nagao, Hideaki Nakagiri, Toshirou Nagaso, Yasue Iwasa, Haruhiko Mori, Makoto Asahina, katsumi imaizumi, MASAO SATO, Unavailability of liver triacylglycerol increases serum cholesterol concentration induced by dietary cholesterol in exogenously hypercholesterolemic (ExHC) rats, Lipids in Health and Disease, 10.1186/1476-511X-13-19, 13, 19-30, 2014.01, Background
Exogenously hypercholesterolemic (ExHC) rats develop hypercholesterolemia and low hepatic triacylglycerol (TAG) levels when dietary cholesterol is loaded. The responsible gene Smek2 was identified via linkage analysis using the original strain Sprague–Dawley (SD) rats. In this study, we compared SD and ExHC rats to investigate a relationship between hypercholesterolemia and the low hepatic TAG levels observed in ExHC rats.
Methods
Male 4-weeks-old ExHC and SD rats were fed a 1% cholesterol diet for 1 week. Serum and liver parameters were analyzed. Gene expression and enzyme activities related to TAG metabolism were also assessed.
Results
We reproducibly observed higher serum cholesterol and lower hepatic TAG levels in ExHC rats than in SD rats. Golgi apparatus in the livers of ExHC rats secreted β-very-low-density lipoprotein (β-VLDL) that had higher cholesterol ester (CE) and lower TAG content than those in the β-VLDL secreted by SD rats. Gene expression related to fatty acid and TAG synthesis in ExHC rats was lower than that in SD rats. Enzymatic activities for fatty acid synthesis were also relatively lower in ExHC rats. Moreover, the fatty acid composition of hepatic and serum CE in ExHC rats showed that these CEs were not modified after secretion from the liver despite the similar activities of serum lecithin-cholesterol acyltransferase (LCAT) in ExHC rats to those in SD rats.
Conclusions
Low production of liver TAG and secretion of CE-rich, TAG-poor β-VLDL without modification by LCAT in the circulation contributed to hypercholesterolemia induced by dietary cholesterol in ExHC rats.
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22. Yukio Kadooka, MASAO SATO, Akihiro Ogawa, Masaya Miyoshi, Hiroshi Uenishi, Hitomi Ogawa, Ken Ikuyama, Masatoyo Kagoshima, Takashi Tsuchida, Effect of Lactobacillus gasseri SBT2055 in fermented milk on abdominal adiposity in adults in a randomized controlled trial, British Journal of Nutrition, 10.1017/S0007114513001037, 110, 9, 1696-1703, PMID: 23614897, 2013.10, Consumption of fermented milk (FM) containing a probiotic, Lactobacillus gasseri SBT2055 (LG2055), previously showed a reduction in abdominal adiposity in a randomised controlled trial (RCT) using FM with 108 colony-forming units (cfu) of LG2055/g. However, whether the effectiveness is observed at lower concentrations, the recommended minimum or intermediate levels of probiotics (106 or 107cfu/g, respectively), remains to be examined. A multi-centre, double-blind, parallel-group RCT was conducted using 210 healthy Japanese adults with large visceral fat areas (80·2-187·8 cm2). They were balanced for their baseline characteristics and randomly assigned to three groups receiving FM containing 107, 106 or 0 (control) cfu LG2055/g of FM, and were asked to consume 200 g FM/d for 12 weeks. Abdominal visceral fat areas, which were determined by computed tomography, at week 12, changed from baseline by an average of - 8·5 % (95 % CI - 11·9, - 5·1; P< 0·01) in the 107 dose group, and by - 8·2 % (95 % CI - 10·8, - 5·7; P< 0·01) in the 106 dose group. Other measures including BMI, waist and hip circumferences, and body fat mass were also significantly decreased from baseline at week 12 in both groups; interestingly, the cessation of taking FM for 4 weeks attenuated these effects. In the control group, none of these parameters significantly decreased from baseline. These findings demonstrate that consumption of LG2055 at doses as low as the order of 108cfu/d exhibited a significant lowering effect on abdominal adiposity, and suggest that constant consumption might be needed to maintain the effect..
23. Ngoc Minh Pham, Shamima Akter, Kayo Kurotani, Akiko Nanri, Masao Sato, Hitomi Hayabuchi, Kazuki Yasuda, Tetsuya Mizoue, Green tea and coffee consumption is inversely associated with depressive symptoms in a Japanese working population, Public Health Nutrition, org/10.1016/j.psychres.2012.04.040, 9, 1, PMID: 23453038, Epub ahead of print, 2013.07, Although several studies have reported an association between blood folate concentrations and
depressive symptoms, few studies have prospectively examined the association. This study aimed to
investigate the cross-sectional and prospective associations between serum folate concentrations and
depressive symptoms among Japanese. We analysed data among 545 subjects who participated in a
health survey at the time of periodic check-up in 2009 for a cross-sectional association and among 272
subjects without depressive symptoms at baseline (in 2006) who responded to both baseline (2006)
and follow-up (2009) surveys for prospective association. Depressive symptoms were assessed using
the Center for Epidemiologic Studies Depression (CES-D) scale. In a cross-sectional analysis, serum
folate concentrations were significantly associated with a decreased prevalence of depressive symptoms
(CES-D scale of Z16). Moreover, serum folate concentrations at baseline were significantly
inversely associated with depressive symptoms after 3 years; the multivariate-adjusted odds ratios
(95% confidence intervals) of depressive symptoms for the lowest through highest tertile categories of
serum folate concentrations were 1.00 (reference), 0.66 (0.29–1.52) and 0.40 (0.16–0.99) (P for
trend¼0.047). Our findings suggest that a higher serum folate may be associated with decreased risk
of depressive symptoms in Japanese..
24. Ayami Kume, Kayo Kurotani, MASAO SATO, Yuko Ejima, Ngoc Minh Pham, Akiko Nanri, Keisuke Kuwahara, Tetsuya Mizoue, Polyunsaturated fatty acids in serum and homocysteine concentrations in Japanese men and women: a cross-sectional study, Nutrition & Metabolism, 10.1186/1743-7075-10-41, 10, 1, Article NO. 41, 2013.07, Background: Supplementation studies have suggested a role of n-3 polyunsaturated fatty acids
(PUFAs) in homocysteine metabolism, but the evidence is limited and inconsistent among studies
that measured blood levels of n-3 and n-6 PUFAs. We examined the association between blood
levels of PUFAs and homocysteine in Japanese men and women.
Methods: The subjects were 496 employees (290 men and 206 women) of 2 municipal offices in
Japan. Fatty acid composition in serum phospholipids and cholesterol ester (CE) was measured
using gas-liquid chromatography. Multiple regression was used to calculate means of homocysteine
concentrations according to PUFA tertile with adjustment for potential confounders.
Results: Serum homocysteine concentration decreased with increasing levels of total n-3 PUFA,
eicosapentaenoic acid and docosahexaenoic acid (DHA) in serum phospholipids and CE with
adjustment for age, sex and workplace. However, only DHA in serum phospholipids remained
statistically significant after additional adjustment for other potential confounders including serum
folate (P-trend = 0.04). N-6 PUFAs were not significantly associated with homocysteine
concentrations.
Conclusions: Higher proportion of DHA in serum phospholipids may be associated with lower
homocysteine concentrations in Japanese men and women..
25. Akiko Nanri, Minh N Pham, Kayo Kurotani, Ayami Kume, Keisuke Kuwahara, MASAO SATO, Hitomi Hayabuchi, Tetsuya Mizoue, Serum pyridoxal concentrations and depressive symptoms among Japanese adults: results from a prospective study, European Journal of Clinical Nutrition, 10.1038/ejcn.2013.115, PMID: 23801094, 2013.07, BACKGROUND/OBJECTIVE: Vitamin B6 is suggested to play a protective role against depression. However, the association between vitamin B6 intake and depression remains inconclusive, and few studies have examined the relationship between circulating vitamin B6 concentrations and depressive symptoms. Here, we investigated the cross-sectional and prospective associations between serum pyridoxal concentrations and depressive symptoms among Japanese workers.
SUBJECTS/METHODS: Participants were 422 municipal employees (aged 21-67 years) who participated in a baseline survey in 2006 for cross-sectional analysis, and 210 subjects without depressive symptoms at baseline (2006) who completed both baseline and follow-up (2009) surveys for prospective analysis. Depressive symptoms were assessed using the Center for Epidemiologic Studies Depression (CES-D) scale. Logistic regression analysis was used to estimate the odds ratio of depressive symptoms (CES-D scale of ≥19) according to tertile of serum pyridoxal with adjustment for potential confounding variables.
RESULTS: In the cross-sectional analysis, serum pyridoxal concentrations were significantly associated with a decreased prevalence of depressive symptoms (P for trend = 0.03); the multivariable-adjusted odds ratio of depressive symptoms for the highest tertile of pyridoxal was 0.54 (95% confidence interval 0.30-0.96) compared with the lowest tertile. In longitudinal analyses, higher serum pyridoxal concentrations at baseline were associated with a trend towards reduced depressive symptoms after three years; the multivariable-adjusted odds ratio of depressive symptoms for the highest versus the lowest tertile of pyridoxal concentration was 0.55 (95% confidence interval 0.13-2.32).
CONCLUSIONS: A higher vitamin B6 status may be associated with a decreased risk of depressive symptoms in Japanese..
26. Keisuke Kuwahara, Akiko Nanri, Minh N Pham, MASAO SATO, Kayo Kurotani, Kazuaki Kawai, Hiroshi Kasai, Tetsuya Mizoue, Serum vitamin B6, folate, and homocysteine concentrations and oxidative DNA damage in Japanese men and women
, Nutrition, 10.1016/j.nut.2013.03.014, 29, 10, 1219-1223, 2013.07.
27. Akter, S, Kurotani, K, Nanri, A., Pham, N.M, MASAO SATO, Hayabuchi, H., Mizoue, T, Dairy consumption is associated with decreased insulin resistance among the Japanese, Nutrition Research, 10.1016/j.nutres.2013.01.009, 33, 4, 286-292, 2013.04, Beneficial effects of dairy consumption in preventing obesity and metabolic syndrome have been suggested, but the association between dairy intake and insulin resistance (IR) is not clear. To test the hypothesis that higher dairy consumption is associated with lower insulin resistance, the authors investigated the association between dairy intake (taking fat content into consideration) and markers of IR in a Japanese working population. A cross-sectional study was conducted in 2009 among 496 subjects aged 20-68 years who participated in a health survey during a periodic checkup. Dairy consumption was assessed using a validated brief dietary history questionnaire. Multiple regression analysis was used to assess the association between fasting serum insulin, plasma glucose, and the homeostatic model assessment of IR (HOMA-IR) and dairy consumption with adjustment for potential confounding variables. The subjects in the highest quartile for total dairy intake showed the lowest mean IR markers compared to those in other categories, although the difference was not statistically significant. Intake of full-fat dairy products was inversely associated with HOMA-IR (P for trend = 0.02). The multivariable adjusted mean HOMA-IR values (with 95% confidence intervals) for the four groups, from those who consumed the fewest to those who consumed the most servings of full-fat dairy products, were 1.04 (0.96-1.12), 1.04 (0.96-1.13), 1.00 (0.91-1.08), and 0.86 (0.76-0.96), respectively. Low-fat dairy intake was not significantly associated with any IR markers. The results suggest that the consumption of full-fat dairy products may be associated with lower IR among Japanese adults..
28. Ngoc Minh Phama, Akiko Nanri, Siyan Yi, Kayo Kurotani,, Shamima Akter, Leng Huat Foo, Nobuo Nishi, MASAO SATO, Hitomi Hayabuchi, Tetsuya Mizoue, Serum ferritin is associated with markers of insulin resistance in Japanese men but not in women, Metabolism: Clinical and Experimental, 10.1016/j.metabol.2012.07.025 , 62, 4, 561-567, 2013.04, OBJECTIVE: Several epidemiological studies have reported that high concentrations of circulating ferritin, a marker of iron stores, are related to insulin resistance (IR); however, questions remain regarding inconsistent data between Asian men and women and the inadequate consideration of potential confounding effects on the relationship between ferritin and IR. Our aim was to examine the relationship between serum ferritin concentrations and IR markers in the Japanese population. MATERIALS/METHODS: We analyzed data (n=493) from a cross-sectional survey conducted in 2009 among a Japanese working population aged 20-68years. Fasting serum ferritin and insulin levels and fasting plasma glucose levels were determined, and the homeostatic model assessment of IR (HOMA-IR) was calculated. Multiple regression analysis was performed with adjustments for demographic and lifestyle factors, body mass index and serum C-reactive protein. RESULTS: Fasting insulin and HOMA-IR significantly increased with increasing levels of serum ferritin after adjustment for covariates in men (P for trend=.005 and .001, respectively). Compared with men in the lowest tertile of serum ferritin, those in the highest tertile had a 24% higher HOMA-IR score. Additional data suggested a positive association between iron intake and HOMA-IR (P for trend=.07) in men. Neither serum ferritin nor iron intake was related to IR markers in women, even in postmenopausal women. CONCLUSIONS: Serum ferritin concentrations were positively associated with fasting insulin and HOMA-IR in men but not in women, suggesting an important role of iron storage in the pathogenesis of IR in Japanese men..
29. Ngoc Minh Pham, Shamima Akter, Kayo Kurotani, Akiko Nanri, MASAO SATO, Hitomi Hayabuchi, Kazuki Yasuda, Tetsuya Mizoue, Serum 25-hydroxyvitamin D and markers of insulin resistance in a Japanese working population, European Journal of Clinical Nutrition, 10.1038/ejcn.2012.169, 66, 12, 1323-1328, PMID: 23093338, 2012.12, Background/Objectives:The association between vitamin D status and insulin resistance (IR) has been less studied among Asians, and it remains elusive whether calcium could modify such an association. We examined the association of serum 25-hydroxyvitamin D (25(OH)D) concentrations with IR markers and the potential effect modification by calcium intake among a Japanese population.
Subjects/Methods:The authors analyzed data (n=494) from a cross-sectional survey conducted in 2009 among a Japanese working population aged 20–68 years. Fasting serum 25(OH)D and insulin, as well as fasting plasma glucose were determined, and homeostatic model assessment of IR (HOMA-IR) was calculated. Calcium intake was assessed using a validated diet history questionnaire. Multiple linear regression was done with adjustment of potential confounding variables.
Results:Fasting insulin and HOMA-IR were significantly inversely associated with 25(OH)D concentration across quartiles of 25(OH)D after fully adjusting for covariates (Ptrend=0.04 and 0.02, respectively). Across clinically relevant categories of 25(OH)D, compared with participants in the vitamin D sufficiency group, those in the vitamin D insufficiency group had a 5% higher HOMA-IR score, and those in the hypovitaminosis D group had an 18% higher HOMA-IR score (Ptrend=0.01). In an analysis by calcium intake, the HOMA-IR score was highest among participants with both a low calcium intake and lowest 25(OH)D concentrations, with significant inverse trend being observed in the group with lower calcium intake (Ptrend=0.02).
Conclusions:Our findings suggest that low vitamin D status is associated with IR among Japanese adults..
30. Akiko Nanri, Hitomi Hayabuchi, Masanori Ohta, MASAO SATO, Norio Mishima, Tetsuya Mizoue, Serum folate and depressive symptoms among Japanese men and women: a cross-sectional and prospective study, Psychiatry Research, 10.1016/j.psychres.2012.04.040, 200, 2-3, 349-353, PubMed ID: 22682152, 2012.12, Although several studies have reported an association between blood folate concentrations and depressive symptoms, few studies have prospectively examined the association. This study aimed to investigate the cross-sectional and prospective associations between serum folate concentrations and depressive symptoms among Japanese. We analyzed data among 545 subjects who participated in a health survey at the time of periodic checkup in 2009 for cross-sectional association and among 272 subjects without depressive symptoms at baseline (in 2006) who responded to both baseline (2006) and follow-up (2009) surveys for prospective association. Depressive symptoms were assessed using the Center for Epidemiologic Studies Depression (CES-D) scale. In a cross-sectional analysis, serum folate concentrations were significantly associated with a decreased prevalence of depressive symptoms (CES-D scale of ≥16). Moreover, serum folate concentrations at baseline were significantly inversely associated with depressive symptoms after three years; the multivariate-adjusted odds ratios (95% confidence intervals) of depressive symptoms for the lowest through highest tertile categories of serum folate concentrations were 1.00 (reference), 0.66 (0.29-1.52), and 0.40 (0.16-0.99) (P for trend = 0.047). Our findings suggest that higher serum folate may be associated with decreased risk of depressive symptoms in Japanese..
31. Bungo Shirouchi, Nakamura Y, Furukawa Y, Shiraishi A, Tomoyori H, katsumi imaizumi, MASAO SATO, Ezetimibe inhibits lymphatic transport of esterified cholesterol but not free cholesterol in thoracic lymph duct-cannulated rats., Cardiovascular Drugs and Therapy, 10.1007/s10557-012-6403-3, 26, 5, 427-431, 2012.10, [URL], Purpose Ezetimibe has been shown to inhibit dietary choles- terol absorption in animal models and humans, but studies on lymphatic lipid transport have not yet been performed. Rats subjected to permanent lymph duct cannulation were used to investigate the effects of ezetimibe on lipid transport. Methods Rats were fed diets with and without ezetimibe (5.0 mg/kg), and their lymph was collected after feeding to quantify lymphatic lipid levels. Total cholesterol content in the intestinal mucosa was also measured.
Results Rats that consumed ezetimibe had significantly lower lymphatic total cholesterol transport with the reduction of esterified cholesterol transport. According to the calculation based on cholesterol consumption, ezetimibe reduced the total cholesterol lymphatic recovery rate by 54 %. We also deter- mined that ezetimibe significantly reduced the total cholester- ol content in the intestinal mucosa.
Conclusion This is the first direct evidence that ezetimibe inhibits esterified but not free cholesterol lymphatic transport in thoracic duct-cannulated rats. The results also indicate that ezetimibe is not involved in the lymphatic transport of triacyl- glycerols, phospholipids, or α-tocopherol..
32. Masako Iwamoto, Katsumi Imai, Hideaki Ohta, Bungo Shirouch and Masao Sato, Supplementation of highly concentrated beta-cryptoxanthin in a satsuma mandarin beverage improves adipocytokine profiles in obese Japanese women, Lipids in Health and Disease, 10.1186/1476-511X-11-52, 11, 1, 52, 2012.09, Background: Serum β-cryptoxanthin levels are lower in overweight subjects than in normal subjects. Abnormalities of adipocytokine profiles in obesity subjects have been reported. There are several reports that serum β-cryptoxanthin levels in them were relatively lower than normal subjects.
Objective: We hypothesize that supplementation of highly concentrated β-cryptoxanthin improves serum adipocytokine profiles in obese subjects. This study tested the association between β-cryptoxanthin intake and serum adipocytokine levels.
Methods: An intervention study consisted of a 3-week long before-and-after controlled trial, where β-cryptoxanthin (4.7 mg/day) was given to 17 moderately obese postmenopausal women.
Results: The results indicated no significant changes in body weight or body mass index (BMI). Serum β-cryptoxanthin levels increased significantly by 4-fold. Serum high molecular weight (HMW)-adiponectin levels increased significantly, while serum plasminogen activator inhibitor (PAI)-1 levels decreased.
Conclusions: We concluded that increasing the intake of β-cryptoxanthin to approximately 4 mg per day for 3 weeks may have beneficial effects on the serum adipocytokine status and consequently alleviate progression of metabolic syndrome.
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33. Kayo Kurotani, MASAO SATO, Yuko Ejima, Nanri, A., Yi, S., Pham, N.M, Akter, S., Poudel-Tandukar, K., Kimura, Y., katsumi imaizumi, High levels of stearic acid, palmitoleic acid, and dihomo-gamma-linolenic acid and low levels of linoleic acid in serum cholesterol ester are associated with high insulin resistance, Nutrition Research, org/10.1016/j.nutres.2012.07.004, 32, 9, 669-675, 2012.07, The association of fatty acid composition with insulin resistance and type 2 diabetes has been reported in Western populations, but there is limited evidence of this association among the Japanese, whose populace consume large amounts of fish. To test the hypothesis that high palmitic, palmitoleic, and dihomo-gamma-linolenic acids and low linoleic and n-3 fatty acids are associated with higher insulin resistance among the Japanese, the authors investigated the relationship between serum fatty acid composition and serum C-peptide concentrations in 437 Japanese employees, aged 21-67 years, who participated in a workplace health examination. Serum cholesterol ester and phospholipid fatty acid compositions were measured by gas-liquid chromatography. Desaturase activity was estimated by fatty acid product-to-precursor ratios. A multiple regression was used to assess the association between fatty acid and C-peptide concentrations. C-peptide concentrations were associated inversely with linoleic acid levels in cholesterol ester and phospholipid (P for trend = 0.01 and 0.02, respectively) and positively with stearic and palmitoleic acids in cholesterol ester (P for trend = 0.02 and 0.006, respectively) and dihomo-gamma-linolenic acid in cholesterol ester and phospholipid (P for trend <0.0001 for both). C-peptide concentrations were not associated with n-3 polyunsaturated fatty acids. C-peptide concentrations significantly increased as delta-9-desaturase (16:1 n-7/16:0; D9D-16) and delta-6-desaturase (18:3 n-6/18:2 n-6; D6D) increased (P for trend = 0.01 and 0.03, respectively) and delta-5-desaturase (20:4 n-6/20:3 n-6; D5D) decreased (P for trend = 0.004). In conclusion, a fatty acid pattern with high levels of serum stearic, palmitoleic, or dihomo-gamma-linolenic acids; D9D-16 or D6D activities; and low levels of serum linoleic acid or D5D activity might be associated with higher insulin resistance in Japanese adults..
34. Yasumi Kimura, Masao Sato, Kayo Kurotani, Akiko Nanri, Kazuaki Kawai, Hiroshi Kasai, Katsumi Imaizumi, Tetsuya Mizoue, PUFAs in serum cholesterol ester and oxidative DNA damage in Japanese men and women, American Journal of Clinical Nutrition, 10.3945/ajcn.111.030817, 95, 1209-1214, 2012.05, Background: PUFAs are susceptible to lipid peroxidation and play a role in inflammation, both of which can induce oxidative stress. However, the relation of PUFA to oxidative DNA damage in humans is elusive.

Objective: We examined the association between concentrations of circulatory PUFAs and urinary 8-oxo-7,8-dihydroguanine (8-oxoGua) in Japanese men and women.

Design: The subjects were 495 participants (290 men and 205 women) in a cross-sectional study in 2 municipal offices in Japan. Serum cholesterol ester (CE) and phospholipid fatty acid composition were measured by gas-liquid chromatography. Urinary 8-oxoGua concentrations were measured by HPLC, and 8-oxoGua values for each tertile of PUFA after adjustment for covariates were calculated by multiple regression.
Results: Urinary 8-oxoGua concentrations increased with increasing concentrations of n–3 (omega-3) PUFAs, EPA, and DHA in serum CE (P-trend = 0.001, 0.01, and 0.009, respectively), whereas they decreased with increasing concentrations of n–6 PUFAs and linoleic acid (P-trend = 0.02 and 0.051, respectively).
Conclusion: Oxidative DNA damage may be greater with higher concentrations of long-chain n–3 PUFAs but lower with higher concentrations of n–6 (omega-6) PUFAs.
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35. Masaki KATO, Yusuke ITO, Yasutake TANAKA, Masao SATO, Katsumi IMAIZUMI, Nao INOUE, and Ikuo IKEDA, SHRSP/Izm and WKY/NCrlCrlj Rats Having Missense Mutation in Abcg5 Deposited Plant Sterols in the Body, But not Change Their Biliary Secretion and Lymphatic Absorption --- Comparison with Jcl:Wistar and WKY/Izm Rats , Bioscience, Biotechnology, and Biochemistry, 2012.03.
36. Tadateru HAMADA, Mariko KUDO, Masao SATO, Nao INOUE, Koji NAGAO, Katsumi IMAIZUMI and Ikuo IKEDA, Effect of a Liver X Receptor Agonist on Deposition and Lymphatic Absorption of Plant Sterols in Stroke-Prone Spontaneously Hypertensive Rats Having a Mutation in ATP-Binding Cassette Transporter G5, Bioscience, Biotechnology, and Biochemistry, 10.1271/bbb.110554, 75, 12, 2359-2363, 2012.03.
37. Kalpana Poudel-Tandukara, Masao Sato, Yuko Ejima, Akiko Nanric, Yumi Matsushitac, Katsumi Imaizumib, Tetsuya Mizoue, Relationship of serum fatty acid composition and desaturase activity to C-reactive protein in Japanese men and women, Atherosclerosis, 0.1016/j.atherosclerosis.2011.11.012 , 220, 2, 520-524, 2012.02, Background: Although fatty acid composition in serum and desaturase activity, which alters serum fatty acid composition, has been associated with C-reactive protein (CRP) concentration in Western populations, no study has been carried out in non-Western populations. We examined the association of serum fatty acids and estimated desaturase activity with CRP concentrations in Japanese men and women. Methods: Subjects were 489 Japanese municipal employees aged 21-67 years who participated in a survey at the time of a periodic health check-up. Serum high-sensitivity CRP concentrations were measured using the latex agglutination nephelometry method. Fatty acid composition was measured in serum cholesteryl esters and desaturase activities by fatty acid product-to-precursor ratios. Relationships were assessed using multiple regression. Results: Serum CRP concentration was positively associated with palmitic acid (P for trend = 0.002) and inversely with alpha-linolenic acid (P for trend = 0.01) in men, and positively with dihomo-gamma-linolenic acid (P for trend in men or women = 0.01) and inversely with delta-5-desaturase (20:4n-6/20:3n-6) (P for trend in men and women = 0.05 and 0.002, respectively) in men and women. Conclusions: Low-grade inflammation may be associated with a serum fatty acid pattern of high palmitic acid or low alpha-linolenic acid in men, and of high dihomo-gamma-linolenic acid or low delta-5-desaturase in both sexes..
38. Li-Tao Tong, Yumiko Fujimoto, Naoki Shimizu, Mariko Tsukino, Taiki Akasaka, Yukiko Kato, Wakako Iwamoto, Sawako Shiratake, Katsumi Imaizumi, Masao Sato, Rice α-globulin decreases serum cholesterol concentrations in rats fed a hypercholesterolemic diet and ameliorates atherosclerotic lesions in apolipoprotein E-deficient mice, Food Chemistry, 10.1016/j.foodchem.2011.10.056, 132, 194-200, 2012.01.
39. Bungo Shirouchi, Sayaka Kawamura, Ryosuke Matsuoka, Sanae Baba, Kazuko Nagata, Sawako Shiratake, Hiroko Tomoyori, Katsumi Imaizumi, and Masao Sato, Dietary guar gum reduces lymph flow and diminishes lipid transport in thoracic duct-cannulated rats, Lipids, DOI: 10.1007/s11745-011-3570-0, 46, 8, 789-793, 2011.08.
40. Masao Sato, Takatoshi Ueda , Kazuko Nagata , Sawako Shiratake , Hiroko Tomoyori , Mitsuo Kawakami , Yukio Ozaki , Hiroshi Okubo , Bungo Shirouchi , Katsumi Imaizumi, Dietary kakrol (Momordica dioica Roxb.) flesh inhibits triacylglycerol absorption and lowers the risk for development of fatty liver in rats, Experimental Biology and Medicine, 10.1258/ebm.2011.011037, 236, 1139-1146, 2011.05.
41. Yukio Kadooka, Akihiro Ogawa, Ken Ikuyama, Masao Sato, The probiotic Lactobacillus gasseri SBT2055 inhibits enlargement of visceral adipocytes and upregulation of serum soluble adhesion molecule (sICAM-1) in rats, International Dairy Journal, 10.1016/j.idairyj.2011.02.001, 2011.04.
42. A Nanri, Y Kimura, Y Matsushita, M Ohta, M Sato, N Mishima, S Sasaki, T Mizoue, Dietary patterns and depressive symptoms among Japanese men and women, European Journal of Clinical Nutrition, 64, 8, 832-839, 2010.08.
43. Yukio Kadooka, Masao Sato, Katsumi Imaizumi, Akihiro Ogawa, Ken Ikuyama, Yoshihito Akai, Masako Okano , Masatoyo Kagoshima, Takashi Tsuchida, Regulation of abdominal adiposity by probiotics (Lactobacillus gasseri SBT2055) in adults with obese tendencies in a randomized controlled trial, European Journal of Clinical Nutrition, 64, 6, 636-643, 2010.06.
44. Makoto Asahina, Waka Haruyama, Yasuhiro Ichida, Mai Sakamoto, Masao Sato, Katsumi Imaizumi , Identification of SMEK2 as a candidate gene for regulation of responsiveness to dietary cholesterol in rats , Journal of Lipid Research, 50:41-46, 2009.01.
45. Ichiko Miyamoto, Jun Shiono, Jie Liu, Kuniyoshi Shimizu, Masao Sato, Akiko Kukita, Toshio Kukita, Ryuichiro Kondo, Regulation of osteoclastogenesis by ganoderic acid DM isolated from Ganoderma lucidum, European Journal of Pharmacology , 6002(1), 1-7, 2009.01.
46. E. M. Hamad, M. Sato, K. Uzu, T. Yoshida, H. Kadooka, H. Kawakami, H. Matsuyama, I. A. A. El-Gawaqd, and K. Imaizumi, Milk fermented by Lactobacillus gasseri SBT2055 influences adipocyte size via inhibition of dietary fat absorption in Zucker rats, Br. J. Nutr., 1-9, 2008.08.
47. M. Sato, K. Uzu, T. Yoshida, E. M. Hamad, H. Kawakami, H. Matsuyama, I. A. A. El-Gawaqd, and K. Imaizumi, Effects of milk fermented by Lactobacillus gasseri SBT2055 on adipocyte size in rats,, Br. J. Nutr., 99, 1013-1017, 2007.12.
48. M. Sato, Y. Kawata· K. Erami,· I. Ikeda· K. Imaizumi, LXR agonist increases the lymph HDL transport in rats by promoting reciprocally intestinal ABCA1 and apo A-I mRNA levels, Lipids, 43 (2), 125-131, 2007.12.
49. M. Sato, H. Sato, A. Ogawa, R. Nomura, S. Takashima, H.-J. Bang, H. Matsuoka and K. Imaizumi, Antiatherogenic effect of isoflavones in ovariectomized apolipoprotein E-deficient mice, J. Agr. Food Chem., 55 (22), 8967–8971, 2007.09.
50. K. Shimizu, M. Yamanaka, M. Gyokusen, S. Kaneko, M. Tsutsui, J. Sato. M. Sato, R. Kondo, Estrogen-like Activity and Prevention Effect of Bone Loss in Calcium Deficient Ovariectomized Rats by the Extract of Pleurotus eryngii, Phytother. Res., 20,659-664, 2006.06.
51. M. Asahina, M. Sato , K. Imaizumi, Genetic analysis of diet-induced hypercholesterolemia in exogenously hypercholesterolemic rats, Journal of Lipid Research, 10.1194/jlr.M5000257-JLR200, 46, 10, 2289-2294, 46: 2289-2294, 2005.10.
Presentations
1. MASAO SATO, Physiological significance of oxidized cholesterols, ACN2015, a joint meeting with The 69th Annual Meeting of Japan Society of Nutrition and Food science, 2015.05.
2. Bungo Shirouchi, Asuka Kawauti, Yumiko Furukawa, Yuko Arima, MASAO SATO, Effects of dietary phosphatidylcholine on postprandial lymphatic inflammatory cytokine levels in rats, ACN2015, a joint meeting with The 69th Annual Meeting of Japan Society of Nutrition and Food science, 2015.05.
3. Yasutake Tanaka, Kiyomi Takahashi, Taiki Akasaka, Naoko Fujita, Yuhi Saito, Bungo Shirouchi, MASAO SATO, Screening of rice (Oryza sativa L.) grains with antidiabetic functionalities, ACN2015, a joint meeting with The 69th Annual Meeting of Japan Society of Nutrition and Food science, 2015.05.
4. Bungo Shirouchi, Asuka Kawauchi, Takuya Kato, Yumiko Furukawa, Yuko Arima, MASAO SATO, Dietary phosphatidylcholine reduces lymphatic inflammatory cytokine levels in thoracic lymph-duct cannulated rats, 1st Asian Conference on Oleo Science (ACOS2014), 2014.09.
5. Bungo Shirouchi, Ryuei Baba, Takuya Kato, Nagao K, MASAO SATO, Dietary Resveratrol Alleviates Atherosclerotic Lesions in ApoE-deficient Mice: Comparison with Pterostilbene, a Resveratrol Analog, 27th International Conference on Polyphenols & 8th Tannin Conference, 2014.09.
6. Tanaka Y, Nagao K, Nakagiri H, Nagaso T, Iwasa Y, Mori H, Asahina M, katsumi imaizumi, MASAO SATO, Increase of Serum Cholesterol Level Induced by Dietary Cholesterol Involves Secretion of Triacylglycerol from Liver in EXHC Rats, IUNS 20th International Congress of Nutrition, 2013.09.
7. Shimabukuro M, 大川知奈美, SATO MASAO, Higashida M, Sato H, Kurobe H, Takao S, Hirata Y, Nakayama T, Lei XF, Kim-Kaneyama JR, Fukuda D, Yamada H, Kitagawa T, Sata M, Effects of Ezetimibe on Oxidized Cholesterol and Fatty-acid Components in Epicardial fat and Myocardium: A Gas Chromatography-Mass Spectrometry Analysis, 第77回日本循環器学会学術集会, 2013.03, Background: Growing evidence suggest that epicardial adipose tissue (EAT) may contribute to the development of coronary artery disease (CAD). Proinflammatory cytokines/adipocytokines are expressed and secreted at a higher level in EAT of individuals with coronary artery disease (CAD) (Hirata et al, JACC 2011). Oxidized cholesterol and fatty acids in food serve as strong atherogenic components, but their distribution in EAT remain unclarified. Objective: We determined effects of ezetimibe on oxidized cholesterol and fatty-acid components in EAT and myocardium using gas chromatography-mass spectrometry analysis.Methods: Male guinea mini pigs were divided into 3 groups of control (80 kcal/kg/day), 1.5% cholesterol-containing high-fat diet (120 kcal/kg/day) (HFD), and HFD+
ezetimibe (0.3mg/kg/day) (Ezetimibe). Fourteen oxidized cholesterol and twenty fatty-acid components were measured by a gas chromatography-mass spectrometry analysis. Results: 1. Plasma levels of Α- and Β-epoxy-cholesterol were increased and 7-ketocholesterol was decreased in HFD group;Α- and Β-epoxy-cholesterol in EAT were decreased in HFD group;Α-epoxy-cholesterol in plasma and epicardial fat were decreased in Ezetimibe group as compared to HFD group, 2. Levels of arachidonic acids were increased comparably in HFD and Ezetimibe groups, 3. EAT volume and echocardiographic indices were comparable among three groups. Conclusion: Components of atherogenic oxidized cholesterol and fatty acids are altered in plasma and perivascular fat and ezetimibe ma
y be beneficial in regulating these components..
8. Dietary guar gum reduces lymph flow and diminishes lipid transport in thoracic duct-cannulated rats..
9. Effects of Lactobacillus gasseri SBT2055 on glucose metabolism in diabetic rats.
10. Positional identification of a gene that regulates serum HDL cholesterol and apolipoprrotein A-I in rats and mice.
11. Atherosclerosis protected by foods -beyond cholesterol-According to a meta-analysis of the reports in Apo E -/- mice-.
Membership in Academic Society
  • The Japanese Society for Food Science and Technology Secretariat
  • Japan Atherosclerosis Society
  • The Japanese Society of Animal Models
  • The Japanese Conference on the Biochemistry of Lipids
  • Japan Society for Bioscience, Biotechnology and Agrochemistry
  • Japanease Society of Nutrition and Food Science
Educational
Educational Activities
In undergraduate education, I have several classes which are "Nutrition Chemistry", " Nutrition physiology", "Nutrition chemistry student experiment" and classes related to those.
In graduate education, I mainly conducted creation of master theses and theses and have advanced classes of "Food Nutrition" and around.