| 1. |
Man Quang LE, Kazuhiro IIYAMA, Haruki NISHIYAMA, Hana OTOFUJI, Yasuhiro SUGA, Kenichi TSUCHIYA, Naruto FURUYA, Whole genome analysis of two Pectobacterium species isolated from blackleg disease of potato occurred in Nagasaki Prefecture, Japan, J. Fac. Agr., Kyushu Univ., 69, 1, 1-10, 2024.02, In Japan, Dickeya dianthicola, D. chrysanthemi, Pectobacterium atrosepticum, P. parmentieri, and P. brasiliense are known as blackleg disease pathogens of potato. A transient outbreak of the blackleg disease occurred in Nagasaki Prefecture, and the pathogens were identified as P. parmentieri and P. brasiliense. Because repetitive extragenic palindromic PCR showed clonal relatedness among strains in each species, two Nagasaki strains (NK5 and NK14) were selected as the representative strains, and their whole genome sequences were determined. Average nucleotide identity analysis supported the identification in the previous study. Multilocus sequence analysis with seven housekeeping genes indicated the relationship between NK14 and other strains isolated in Japan, Russia, China, Switzerland, Poland, and Belgium. NK5 was related to the strains from various geographic locations including Syria, China, Canada, South Korea, Netherlands, Algeria, South Africa, Mexico, and Belarus. Subsequently, core genome multilocus sequence typing was performed with publicly available sequences, indicating that NK14 and NK5 were most closely related to the strains Poland/Russian and Netherlands/Belarus/Russia, respectively. This study concerns the first phylogenetic analyses based on the whole genome sequences of Japanese strains of P. parmentieri and P. brasiliense, and contributes to the molecular epidemiological analysis of the pathogen of potato blackleg.
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| 2. |
Kazuhiro Iiyama, Honoka Kusakabe, Natsuki Juukurogi, Monami Takehara, Ryota Sakai, Shun Yonehara, Mitsuo Horita, Kenichi Tsuchiya, Naruto Furuya , Prediction of gene clusters associated with host specificity of Ralstonia pseudosolanacearum race 4 based on comparative genomic analysis., Journal of General Plant Pathology, 10.1007/s10327-023-01150-4, 89, 328-332, 2023.09. |
| 3. |
Man Quang LE, Kazuhiro IIYAMA, Yasuhiro SUGA, Hana OTOFUJI, Kenichi TSUCHIYA, Naruto FURUYA, Isolation and identification of the causal agents of blackleg disease of potato occurred in Nagasaki Prefecture, Japan, J. Fac. Agr., Kyushu Univ., 68, 1, 13-19, 2023.02. |
| 4. |
Tomomi Inoue, Kazuhiro Iiyama, Moe Asakuma, Momoka Imamura, Shun Yonehara, Htet Wai Wai Kyaw, Kazutaka Yano, Kenichi Tsuchiya, Naruto Furuya, Screening and risk assessment of biological control agents against bacterial wilt of ginger, Biological Control, 10.1016/j.biocontrol.2022.105059, 175, 105059, 2022.09. |
| 5. |
Kazuhiro Iiyama, Ryo Michishita, Hikaru Arima, Htet Wai Wai Kyaw, Kazutaka Yano, Mitsuo Horita, Kenichi Tsuchiya, Naruto Furuya, Possible invasion pathway of Ralstonia pseudosolanacearum race 4 in ginger plant, Journal of General Plant Pathology, 10.1007/s10327-022-01072-7, 88, 246-252, 2022.07. |
| 6. |
Htet Wai Wai Kyaw, Kazuhiro Iiyama, Shun Yonehara, Tin Aye Aye Naing and Naruto Furuya, Occurrence of bacterial wilt disease of ginger caused by Ralstonia solanacearum species complex in Myanmar, J. Fac. Agr., Kyushu Univ., 67, 2, 129-132, 2022.06. |
| 7. |
Kazuhiro Iiyama, Momoka Imamura, Tomomi Inoue, Htet Wai Wai Kyaw, Kazutaka Yano, Mitsuo Horita, Kenichi Tsuchiya, Naruto Furuya, Pathogenicity evaluation of Ralstonia pseudosolanacearum race 4 on ginger by leaf-clipping inoculation, Journal of General Plant Pathology, 10.1007/s10327-021-01017-6, 87, 269-272, 2021.07. |
| 8. |
Kazuhiro Iiyama, Sayo Tani, Haruka Yagi, Sara Hashimoto, Yasuhiro Suga, Kenichi Tsuchiya, Naruto Furuya , d-Tartrate utilization correlates with phylogenetic subclade in Pseudomonas cichorii, FEMS microbiology letters, 10.1093/femsle/fnaa223, 368, 2, 2021.01. |
| 9. |
Mai Morishita, Hiroaki Mon, Jae Man Lee, Takahiro Kusakabe, Maximiano Corrêa Cassal, Chisa Yasunaga-Aoki and Kazuhiro Iiyama, Toxin complex is a major virulence determinant of Enterobacter sp. 532 against the silkworm, Bombyx mori, J. Insect Biotechnol. Sericol. , 88, 1, 21-25, 2019.02. |
| 10. |
Kenji Kikuhara, Kazuhiro Iiyama, Masaru Matsumoto, Naruto Furuya, First report on DMI fungicide resistance in Gymnosporangium asiaticum, the causal agent of Japanese pear rust, in Japan, JOURNAL OF GENERAL PLANT PATHOLOGY, 10.1007/s10327-018-0820-5, 85, 1, 49-56, 2019.01, Japanese pear rust, caused by Gymnosporangium asiaticum Miyabe ex Yamada, is one of the most important fungal plant diseases affecting Japanese pear cultivation. Although sterol demethylation inhibitor (DMI) fungicides have controlled this disease effectively, outbreaks have been frequent in some areas of Japan since 2008. We, therefore, evaluated fungal populations from Fukuoka Prefecture for DMI fungicide sensitivity in vivo. Aeciospores on diseased pear leaves were collected in 2013 from four orchards in Fukuoka Prefecture and one control orchard in Ibaraki Prefecture. Juniperus chinensis var. kaizuka Hort. test plants were sprayed with difenoconazole and fenarimol at recommended rates, and the plants were inoculated with aeciospores. Difenoconazole and fenarimol effectively controlled the population of rust fungus from Ibaraki Prefecture. In contrast, fenarimol and difenoconazole were found to be ineffective against fungal populations from Fukuoka Prefecture. Subsequently, telia were collected from diseased juniper twigs in five areas in Fukuoka Prefecture and one control area in Ibaraki Prefecture in 2013 and 2014. Similarly, test plants of Japanese pear ‘Kousui’ were treated with difenoconazole or fenarimol and inoculated with basidiospores taken from those telia. Both difenoconazole and fenarimol were largely ineffective against the populations from Fukuoka Prefecture. In addition, field trials conducted in an experimental orchard in 2014 and 2015 showed that difenoconazole and fenarimol were not effective in controlling this disease. These results indicate the occurrence of Japanese pear rust resistant to DMI fungicides in Fukuoka Prefecture.. |
| 11. |
Mai Morishita, Akitsu Masuda, Hiroaki Mon, Jae Man Lee, Takahiro Kusakabe, Kosuke Tashiro, Chisa Yasunaga-Aoki, Kazuhiro Iiyama, Identification of insecticidal toxin produced by Enterobacter sp. strain 532 isolated from diseased Bombyx mori silkworms, FEMS Microbiology Letters, 366, 2, fny295, 2019.01, Although Enterobacter sp. 532 shows pathogenicity in Bombyx mori, the insecticidal mechanisms are unclear. Here, we identified and characterised an insecticidal protein from Enterobacter. The insecticidal protein was purified from the strain and inoculated into B. mori larvae. Intracellular proteins were prepared, purified and separated by preparative native polyacrylamide gel electrophoresis (PAGE); one protein band had insecticidal activity. Sodium dodecyl sulfate-PAGE showed the presence of several bands, indicating that the insecticidal protein formed a complex. Peptide mass fingerprinting of a prominent 255.3-kDa band revealed 64 peptides that matched one protein with 33.0% sequence coverage. This protein was a homologue of the A component of the toxin complex (Tc), and the VRP1 domain was conserved; thus, the gene was named itcA (insecticidal toxin complex A). In the itcA downstream region, B and C component gene homologues were found, and these genes were located on an 86.2-kb contig sequence. Two repA genes and 27 genes related to conjugation transfer of plasmids were located on the contig, suggesting that the contig originated from a mobilisable plasmid. Therefore, these findings suggested that the strain may have acquired the Tc genes by horizontal transfer. This is the first description of Tc produced by the genus Enterobacter.. |
| 12. |
Shahadat Hossain, Abu Ashraf Khan, Md Rahman, Kazuhiro Iiyama, Naruto Furuya, First Report of Soft Rot Disease of Papaya Caused by Klebsiella variicola in Bangladesh, J. Fac. Agr., Kyushu Univ., 63, 2, 201-205, 2018.12. |
| 13. |
Htet Wai Wai Kyaw, Kenichi Tsuchiya, Masaru Matsumoto, Kazuhiro Iiyama, Seint San Aye, Myo Zaw, Daisuke Kurose, Mitsuo Horita, Naruto Furuya, Genetic diversity of Ralstonia solanacearum strains causing bacterial wilt of solanaceous crops in Myanmar, J Gen Plant Pathol, 59, 221-233, 2017.07, In 2013 and 2014, an extensive survey of bacterial wilt in Myanmar was performed, and 70 strains of Ralstonia solanacearum (Rs) were collected from wilting plants of tomato, potato, chili and eggplant. Myanmar Rs strains were characterized by traditional and molecular methods. Polymerase chain reaction (PCR) test using Rs-specific primer set amplified one specific band (281-bp) from template DNA of all strains. Pathogenicity tests on the four solanaceous plants differentiated the strains into six pathogenic groups. Biovar determination tests showed that biovar 3 strains predominated (63%) among all Rs strains. Biovar 4 strains (7%) were obtained from both tomato and chili strains, whereas biovar 2 (30%) strains were isolated only from potato. Multiplex-PCR analysis indicated that tomato, eggplant and chili strains belonged to phylotype I, whereas potato strains comprised phylotype I and phylotype II. Strains in phylotype I, which was suggested to have originated from Asia, were the most prevalent in all surveyed areas. Phylogenetic analysis based on the endoglucanase (egl) gene sequences revealed that Myanmar strains partitioned into two major clusters that corresponded to phylotype I and II. Strains in phylotype I were further divided into seven subclusters, each corresponding to a distinct sequevar (15, 17, 46, 47, 48, unknown 1 or unknown 2). All strains in phylotype II belonged to sequevar 1. This is the first comprehensive report of the presence of diverse Rs strains in Myanmar.. |
| 14. |
Kazuhiro Iiyama, Eigo Takahashi, Jae Man Lee, Hiroaki Mon, Mai Morishita, Takahiro Kusakabe, Chisa Yasunaga-Aoki, Alkaline protease contributes to pyocyanin production in Pseudomonas aeruginosa., FEMS Microbiology Letters, doi.org/10.1093/femsle/fnx051, 364, fnx051, 2017.04. |
| 15. |
Kazuhiro Iiyama, Mai Morishita, Jae Man Lee, Hiroaki Mon, Takahiro Kusakabe, Kosuke Tashiro, Taiki Akasaka, Chisa Yasunaga-Aoki and Kazuhisa Miyamoto, A reconsideration of the taxonomic position of two bacterial strains isolated from flacherie-diseased silkworms in 1965, Journal of Insect Biotechnology and Sericology, 86, 35-41, 2017.04, Recent advances in bacterial characterization methodologies have made taxonomic categorization significant- ly more accurate. Here, we re-evaluated the position of bacterial strains (532 and 652) belonging to the genus Hafnia, isolated from flacherie-diseased silkworms in 1965. Phylogenetic analysis based on the 16S rRNA gene sequences of these strains suggests that they belong to genus Enterobacter. Using multilocus sequence analy- sis (MLSA), these strains were further classified to MLSA group A, which is a “core” group of Enterobacter con- taining E. cloacae (the type species of the genus). Although these strains were closely related to E. mori, E. tabaci, and E. asburiae, they also had other MLSA characteristics that distinguished them from these neighbor- ing bacterial species. These data were supported by further biochemical analysis. Thus, it appears that the 532 and 652 strains isolated almost half a century ago belong to genus Enterobacter, and their unique characteristics strongly suggest that they are a novel bacterial species.. |
| 16. |
Erika Taira, Hiroaki Mon, Jae Man Lee, Takahiro Kusakabe, Chisa Yasunaga-Aoki, Kazuhiro Iiyama, Virulence of lipopolysaccharide-deficient mutants of Serratia liquefaciens toward the silkworm, Bombyx mori., Journal of Insect Biotechnology and Sericology, 10.11416/jibs.85.1_007, 85, 7-14, 2016.04. |
| 17. |
Eigo Takahashi, Jae Man Lee, Hiroaki Mon, Yuuka Chieda, Chisa Yasunaga-Aoki, Takahiro Kusakabe, Kazuhiro Iiyama, Effect of antibiotics on extracellular protein level in Pseudomonas aeruginosa., Plasmid, 10.1016/j.plasmid.2016.03.001, 84-85, 44-50, 2016.03. |
| 18. |
Oumi Nishi, Kazuhiro Iiyama, Chisa Yasunaga-Aoki, Susumu Shimizu, Phylogenetic status and pathogenicity of Metarhizium majus isolated from a fruit beetle larva in Japan, Mycological Progress, 10.1007/s11557-015-1082-7, 14, 8, 58-58, 2015.08. |
| 19. |
Kazuhiro Iiyama, Hiroaki Mon, Kazuki Mori, Takumi Mitsudome, Jae Man Lee, Takahiro Kusakabe, Kousuke Tashiro, Shin-Ichiro Asano, Chisa Yasunaga-Aoki, Characterization of KfrA proteins encoded by a plasmid of Paenibacillus popilliae ATCC 14706T., Meta Gene, 10.1016/j.mgene.2015.03.001, 4, 29-44, 2015.06. |
| 20. |
Erika Taira, Kazuhiro Iiyama, Hiroaki Mon, Kazuki Mori, Taiki Akasaka, Kousuke Tashiro, Chisa Yasunaga-Aoki, Jae Man Lee, Takahiro Kusakabe, Draft genome sequence of entomopathogenic Serratia liquefaciens strain FK01., Genome Announcements, 2, 3, e00609-14-e00609-14, 2014.06. |
| 21. |
Kazuhiro Iiyama, Masahiro Otao, Kazuki Mori, Hiroaki Mon, Jae Man Lee, Takahiro Kusakabe, Kousuke Tashiro, Shin-Ichiro Asano, Chisa Yasunaga-Aoki, Phylogenetic relationship of Paenibacillus species based on putative replication origin regions and analysis of an yheCD-like sequence found in this region., Bioscience, Biotechnology, and Biochemistry, 10.1080/09168451.2014.905188, 78, 891-897, 2014.05. |
| 22. |
Oumi Nishi, Kazuhiro Iiyama, Chisa Yasunaga-Aoki, Susumu Shimizu, Comparison of the germination rates of Metarhizium spp. conidia from Japan at high and low temperatures, Letters in Applied Microbiology, 10.1111/lam.12150, 57, 6, 554-560, 2013.12. |
| 23. |
Mon Hiroaki, JAE MAN LEE, Fukushima Mai, Nagata Yudai, Fujii Mie, Xu Jian, Nishi Oumi, Kazuhiro Iiyama, takahiro kusakabe, Production and characterization of the celery mismatch endonuclease CEL II using baculovirus/silkworm expression system, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 10.1007/s00253-012-4583-1, 97, 15, 6813-6822, 2013.08. |
| 24. |
Kazuhiro Iiyama, Kazuki Mori, Hiroaki Mon, Yuuka Chieda, Jae Man Lee, Takahiro Kusakabe, Kosuke Tashiro, Shin-ichiro Asano, Chisa Yasunaga-Aoki, Susumu Shimizu, Draft Genome Sequence of Paenibacillus popilliae ATCC 14706T, Journal of Insect Biotechnology and Sericology, 82, 2, 45-48, 2013.06. |
| 25. |
Kazuhiro Iiyama, Oumi Nishi, Hiroaki Mon, JAE MAN LEE, takahiro kusakabe, Asano Shin-ichiro, Chisa Yasunaga-Aoki, Susumu Shimizu, Phylogenetic analysis of Paenibacillus popilliae and its related taxa based on housekeeping genes., J. Insect Biotechnol. Sericol. , 10.11416/jibs.82.1_001, 82, 1-11, 2013.02. |
| 26. |
Arai Hiroyuki, Iiyama Kazuhiro, Role of nitric oxide-detoxifying enzymes in virulence of Pseudomonas aeruginosaagainst the silkworm, Bombyx mori, Biosci. Biotechnol. Biochem, 77, 1, 198-200, 2013.01. |
| 27. |
Kaibara Fusako, Iiyama Kazuhiro, Chieda Yuuka, Lee Jae Man, kusakabe takahiro, Yasunaga-Aoki Chisa, Shimizu Susumu, Construction of serralysin-like metalloprotease-deficient mutants of Serratia liquefaciens and their virulence in the silkworm, Bombyx mori, J. Insect Biotechnol. Sericol. , 81, 55-61, 2012.12. |
| 28. |
Chieda, Y., Iiyama, K., Lee, J.M., Kusakabe, T., Yasunaga-Aoki, C. and Shimizu, S., Virulence of an exotoxin A-deficient strain of Pseudomonas aeruginosa toward the silkworm, Bombyx mori., Microbial Pathogenesis, 51, 6, 407-414, 2011.12. |
| 29. |
Iiyama, K., Lee, J.M., Kusakabe, T., Yasunaga-Aoki, C. and Shimizu, S. , Improvement in pHERD vectors to express recombinant proteins tagged with hexahistidine at either the NH2- or COOH- terminal in Pseudomonas aeruginosa, J. Insect Biotechnol. Sericol., 80, 57-61, 2011.06. |
| 30. |
Egami, I., Iiyama, K., Zhang, P., Chieda, Y., Ino, N., Hasegawa, K., Lee, J.M., Kusakabe, T., Yasunaga-Aoki, C. and Shimizu, S., Insecticidal bacterium isolated from an ant lion larva from Munakata, Japan., J. Appl. Entomol., vol. 133 No. 2 117-124, 2009.03. |
| 31. |
Iiyama, K., Chieda, Y., Lee, J.M., Kusakabe, T., Yasunaga-Aoki, C. and Shimizu, S., Virulence of Phospholipase C Mutants of Pseudomonas aeruginosa PAO1 against the Silkworm, Bombyx mori., J. Insect Biotechnol. Sericol., vol. 77, No. 2, 115-120, 2008.06. |
| 32. |
Chieda, Y., Iiyama, K., Lee, J.M., Kusakabe, T., Yasunaga-Aoki, C. and Shimizu, S., Inactivation of pyocyanin synthesis genes has no effect on the virulence of Pseudomonas aeruginosa PAO1 toward the silkworm, Bombyx mori, FEMS Microbiology Letters, 278 101-107, 2008.01. |
| 33. |
Chieda, Y., Iiyama, K., Lee, J.M., Kusakabe, T., Yasunaga-Aoki, C. and Shimizu, S., The gacA gene of Pseudomonas aeruginosa PAO1 is not required for full virulence in Bombyx mori., J. Insect Biotechnol. Sericol. , Vol. 76, 89-95, 2007.06. |
| 34. |
Iiyama K, Chieda Y, Lee JM, Kusakabe T, Yasunaga-Aoki C, Shimizu S., Effect of superoxide dismutase gene inactivation on virulence of Pseudomonas aeruginosa PAO1 toward the silkworm, Bombyx mori, Appl Environ Microbiol., 73(5):1569-75, 2007.03. |
| 35. |
Chieda, Y., Iiyama, K., Yasunaga-Aoki, C., Lee, J.M., Kusakabe, T. and Shimizu, S., Pathogenicity of gacA mutant of Pseudomonas aeruginosa PA01 in the silkworm, Bombyx mori, FEMS Microbiol. Lett., 10.1016/j.femsle.2005.01.032, 244, 1, 181-186, 244巻 181-186, 2005.03. |
| 36. |
Iiyama, K., Chieda, Y., Yasunaga-Aoki, C., Hayasaka, S. and Shimizu, S., Analyses of the ribosomal DNA region in Nosema bombycis NIS 001., J. Eukaryot. Microbiol, 10.1111/j.1550-7408.2004.tb00592.x, 51, 6, 598-604, 51巻 598-604., 2004.01. |
