Kyushu University Academic Staff Educational and Research Activities Database
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KOHJI YAMAMOTO Last modified date:2019.06.14



Graduate School
Other Organization


Homepage
http://www.agr.kyushu-u.ac.jp/lab/sanshi/insect/
web site for the laboratery of insect genetic resources .
Academic Degree
Ph.D. in Biochemistry
Country of degree conferring institution (Overseas)
No
Field of Specialization
Structural Biology, Applied entomology, Enzymology, Biochemistry
Total Priod of education and research career in the foreign country
03years00months
Outline Activities
Research Interests
(a) The resistance to oxidative stress of insect: Active oxygen such as superoxide anion and hydrogen dioxide is highly reactive and is toxic to organisms. However, the protective reaction against oxidative stress has not characterized in the silkworm, Bombyx mori. Catalase is one of antioxidant enzymes and produces water and oxygen from hydrogen dioxide. To elucidate biochemical aspects of the resistance to oxidative stress, we have cloned and sequenced gene encoding catalase from the silkworm, Bombyx mori.

(b) DNA/RNA binding protein in insect: During metamorphosis in insect, it is known that gene expressions are regulated. DNA/RNA binding proteins are thought be responcible for the regulation. We focus on Y-box protein, RNA binding protein and MCM helicase, DNA binding protein. we have analyzed the structure and function of these proteins.
Research
Research Interests
  • Detoxification enzymes in lepidopteran
    keyword : glutathione S-transferase, sulfotransferase, cytochrome P450
    2005.04~2020.03.
  • Antioxidant enzymes in insects
    keyword : catalase, superoxide dismutase, reactive oxygen species, oxidative stress
    2003.04~2020.03Active oxygen such as superoxide anion and hydrogen dioxide is highly reactive and is toxic to organisms. However, the protective reaction against oxidative stress has not characterized in the silkworm, Bombyx mori. Catalase is one of antioxidant enzymes and produces water and oxygen from hydrogen dioxide. To elucidate biochemical aspects of the resistance to oxidative stress, we have cloned and sequenced gene encoding catalase from the silkworm, Bombyx mori..
  • DNA/RNA-binding proteins
    keyword : DNA/RNA-binding proteins, MCM
    2002.05~2020.03During metamorphosis in insect, it is known that gene expressions are regulated. DNA/RNA binding proteins are thought be responcible for the regulation. We focus on Y-box protein, RNA binding protein and MCM helicase, DNA binding protein. we have analyzed the structure and function of these proteins..
  • DNA replication initiation
    keyword : DNA replication, DnaA, DnaB, intein
    1999.02~2017.03I have studied on DNA replication initiation in mycobacteria. DnaA, initiater protein, was cloned and sequenced. Also, Overexpression and purification of DnaA was constructed. Interaction between the expressed DnaA and oriC has been investigated..
  • Enzymes related to skin tanning in insects
    keyword : Phenoloxidase, Dopachrome conversion enzyme
    1993.04~2020.03.
Academic Activities
Books
1. Kohji Yamamoto, Pingbo Zhang, Yutaka Banno and Hiroshi Fujii, Recent Research Developments in Bioenergetics, TRANSWORLD RESEARCH NETWORK, vol4, chapter 2 "Antioxidant defense in the silkworm, Bombyx mori", p33-42, 2006.01.
Reports
1. DNA replication initiation in Mycobacteria.
2. The mycobacterium avium-intracellulare complex dnaB locus and protein intein splicing.
Papers
1. Haque MR, Higashiura A, Nakagawa A, Hirowatari A, Furuya S, Yamamoto K, Molecular structure of a 5,10-methylenetetrahydrofolate dehydrogenase from the silkworm Bombyx mori, FEBS Open Bio, 9, 618-628, 2019.01.
2. Cheng T.*, Wu J.*, Wu Y.*, Chilukuri R.*, Huang L.*, Yamamoto K.*, Feng L.*, Li W.* et al., Arunkumar KP., Kishino H., Goldsmith MR., Feng Q., Xia Q., Mita K., Wide distribution of a major East Asian noctuid pest explained by genomic adaptation to polyphagy and insecticides, Nature Ecology & Evolution, *equal contribution, 1 (11), 1747-1756, 2017.07, 重要農業害虫であるハスモンヨトウ(Spodoptera litura)のゲノム塩基配列を解読した。本昆虫ゲノム中には、苦味物質ならびに有毒物質の受容体、シトクロムP450、カルボキシルエステラーゼそしてグルタチオン-Sトランスフェラーゼなどの解毒酵素をコードする複数の遺伝子ファミリーが多数存在しており、多くの生体異物や植物二次化合物を検知および解毒できる能力を備えていることを見いだした。.
3. Yamamoto K., Higashiura A., Suzuki M., Aritake K., Urade Y., Nakagawa A., Molecular structure of a prostaglandin D synthase requiring glutathione from the brown planthopper, Nilaparvata lugens, Biochemical and Biophysical Research Communications, 492, 166-171, 2017.07.
4. Yamamoto K., Yamada N., Identification of a diazinon-metabolizing glutathione transferase in the silkworm, Bombyx mori, Scientific Reports, 6, 30073, 2016.03.
5. Yamamoto K., Higashiura A., Suzuki M., Shiotsuki T., Sugahara R., Fujii T., Nakagawa A., Structural characterization of an aldo-keto reductase (AKR2E5) from the silkworm Bombyx mori, Biochemical and Biophysical Research Communications, 474, 104-110, 2015.12.
6. Yamamoto K, Suzuki M, Higashiura A, Aritake K, Urade Y, Uodome N, Hossain MDT, Nakagawa A, New insights into the catalytic mechanism of Bombyx mori prostaglandin E synthase gained from structure-function analysis, Biochemical and Biophysical Research Communications, 440, 762-767, 2013.10.
7. Yamamoto K, Szuki M, Higashiura A, Nakagawa A, Three-dimensional structure of a Bombyx mori Omega-class glutathione transferase, Biochemical and Biophysical Research Communications, 438, 588-593, 2014.03.
8. Yamamoto K, Wilson DK., Identification, characterization, and crystal structure of an aldo-keto reductase (AKR2E4) from the silkworm Bombyx mori, Archives of Biochemistry and Biophysics, 538, 156-163, 2013.10.
9. Yamamoto K, Aso Y, Yamada N, Catalytic function of an Epsilon-class glutathione transferase of the silkmoth
, Insect Mol. Biol., 22, 5, 523-531, 2013.04.
10. Yamamoto K, Higashiura A, Suzuki M, Aritake K, Urade Y, Uodome N, Nakagawa A, Crystal structure of a Bombyx mori Sigma-class glutathione transferase exhibiting prostaglandin E synthase activity, Biochim Biophys Acta—General Subjects, 1830, 3711-3718, 2013.02.
11. Yamamoto K, Usuda K, Kakuta Y, Kimura M, Higashiura A, Nakagawa A, Aso Y, Suzuki M, Structural basis for catalytic activity of a silkworm Delta-class glutathione transferase. , Biochim Biophys Acta-General Subjects, 1820, 1469–1474, 2012.07.
12. Yamamoto K, Teshiba S, Shigeoka Y, Aso Y, Banno Y, Fujiki T, Katakura Y, Characterization of an omega-class glutathione S-transferase in the stress response of the silkmoth, Insect Mol. Biol., 20, 3, 379-386, 2011.05.
13. Yamamoto K, Ichinose H, Aso Y, Banno Y, Kimura M, Nakashima T, Molecular characterization of an insecticide-induced novel glutathione transferase in silkworm. , Biochim Biophys Acta-General Subjects, 1810 , 420–426, 2011.04, Glutathione transferase (GST) は代表的な農薬解毒酵素である。頻用農薬ダイアジノンに対するカイコ耐性ならびに感受性系統間においてunclassified GST (GSTu) mRNAの発現量に差があることがマイクロアレイ分析により判明した。大腸菌により組換えGSTuを作製したところ、本酵素は、酸化ストレス誘発剤・1-chloro-2,4-dinitrobenzeneへの還元グルタチオン(GSH)転移活性を有していた。部位特異的アミノ酸変異分析の結果、N末端側のTyr7, Ser12そしてAsn50が活性発現に重要であることが分かった。以上の結果より、カイコGSTuは、農薬耐性に関与している可能性が示唆された。.
14. Yamamoto K, Muniruzzaman S, Rajagopalan M, Madiraju M, Modulation of Mycobacterium tuberculosis DnaA protein-adenine nucleotide interactions by acidic phospholipids, Biochem J., 363, 305-311, 2002.01.
15. Yamamoto K, Low B, Rutherford SA, Rajagopalan M, Madiraju M, The Mycobacterium avium-intracellulare complex dnaB locus and protein intein splicing, Biochem. Biophys. Res. Commun., 280, 898-903, 2001.01.
16. Yamamoto K, Yakiyama M, Fujii H, Kusakabe T, Koga K, Aso Y, Ishiguro M, Expression of prophenoloxidase mRNA during silkworm hemocyte development : Developmental changes of prophenoloxidase, Biosci. Biotech. Biochem., 10.1271/bbb.64.1197, 64, 6, 1197-1202, 2000.06.
Works, Software and Database
1. Proteome database in the silkworm
[URL].
Presentations
1. Kohji Yamamoto, David K. Wilson, Identification and characterization of a novel AKR from the silkworm, Bombyx mori, Carbonyl conference 2018, 2018.07.
2. Characterization of glutathione S-transferases of the silkworm and the fall webworm.
3. Antioxidant defense in the silkworm, Bombyx mori.
4. Cloning and charcterization of glutathione S-transferase from the silkworm.
5. Superoxide dismutase from the silkworm, Bombyx mori.
6. Catalase from the silkworm, Bombyx mori.
7. Cloning, sequencing and expression of gene encoding catalase from the silkworm, Bombyx mori.
8. The dnaA gene region of Mycobacterium avium and the autonomous replication activity.
9. Expression, Purification and characterization of DnaA of Mycobacterium tuberculosis.
Membership in Academic Society
  • Pesticide Science Society of Japan
  • Japan Society for Bioscience, Biotechnology, and Agrochemistry
  • The Japanese Society of Sericultural Science
  • The Japanese Biochemical Society