|kuniyoshi shimizu||Last modified date：2019.09.18|
Associate Professor / Systematic Forest and Forest Products Science / Department of Agro-environmental Sciences / Faculty of Agriculture
|1.||Isao Kubo, Kuniyoshi Shimizu, Ken Ichi Nihei, Maniçoba, a quercetin rich amazonian dish
Antioxidative prospects, Nova Science Publishers, Inc., 129-154, 2012, A large quantity of quercetin was characterized from a local dish, known as maniçoba in Pará, Brazil. The fresh leaf of Manihot esculenta Crantz (Euphorbiaceae), a principal ingredient for maniçoba, was found to contain a large amount of rutin or quercetin 3-O-rutinoside which was completely hydrolyzed to quercetin during cooking processes. Antioxidant activity of quercetin and its microfloral metabolites was examined. Quercetin inhibited the oxidation of linoleic acid catalyzed by soybean lipoxygenase-1 (EC 18.104.22.168, Type 1) with an IC50 of 4.5 μM, while rutin did not inhibit this oxidation up to 200 μM. The progress curves for the enzyme reaction indicate that quercetin shows slow binding kinetics. In the pseudoperoxidase activity, hydroperoxides slowly oxidize quercetin to a rather stable intermediate, 2-(3,4-dihydroxybenzoyl)-2,4,6-trihydroxy-3(2H)-benzofuranone. A microfloral metabolite of quercetin and rutin after ingestion, 3,4-dihydroxyphenylacetic acid, also scavenged superoxide anion generated by xanthine oxidase and inhibited uric acid formation. The antioxidant activity observed with the methanol extract of maniçoba is due mainly to multifunctional quercetin's response..
|2.||I. Kubo, K. Shimizu, K. Fujita, Naturally Occurring Antifungal Agents and Their Modes of Action.
Fungicides for Plant and Animal Diseases, InTech, D. Dhanasekaran, N. Thajuddin and A. Panneerselvam (Ed.), 2012.11, [URL].
|3.||I. Kubo, K. Shimizu, K. Nihei, MANICOBA, A quercetin rich Amazonian dish
In: Quercetin: Dietary Sources, Functions and Health Benefits, Nova Science Publishers, Inc., 2012.11.
|4.||I. Kubo, K. Fujita, K. Shimizu, Anti-Salmonella Agents from Tanacetum Balsamita, A Brazilian Medicinal Plant, and their Application
In: Antioxidants and Biocides from Latin American Plants, CABI Publishing (UK), C.L. Cespedes, D.A. Sampietro, D.S. Seigler, Ed., 2012.11.
|5.||N. Masuoka, K. Shimizu, I. Kubo, Antioxidant Activity of Anacardic Acids
In: Antioxidants and Biocides from Latin American Plants;
, CABI Publishing (UK), C.L. Cespedes, D.A. Sampietro, D.S. Seigler, Ed., 2012.11.
|6.||I. Kubo, T. J. Ha, K. Shimizu, Molecular Design of Soybean Lipoxygenase Inhibitors Based on Natural Products, A Comprehensive Survey of International Soybean Research - Genetics, Physiology, Agronomy and Nitrogen Relationships, InTech, James E. Board (Ed.)
DOI: 10.5772/52703., 2013.03, [URL].
|7.||Isao Kubo, Ken Ichi Fujita, Kuniyoshi Shimizu, Anti-salmonella agents from the Brazilian medicinal plant tanacetum balsamita and their applications, CABI Publishing, 239-253, 2013.04.|
|8.||Noriyoshi Masuoka, Kuniyoshi Shimizu, Isao Kubo, Antioxidant activity of anacardic acids, CABI Publishing, 137-147, 2013.04.|
|9.||Amira Mira, Kuniyoshi Shimizu, An update on antitumor activity of Angelica species, Springer Singapore, 10.1007/978-981-10-1085-9_15, 363-371, 2016.01, Genus Angelica (Family: Apiaceae) comprises more than 60 species of medicinal plants. Many of these species have long been used in ancient traditional medicine systems, especially in the Eastern countries. For many years, several species of Angelica, e.g., A. archangelica, A. dahurica, A. keiskei, A. gigas, A. pubescens, A. sinensis, A. shikokiana have been used traditionally as anti-inflammatory, diuretic, expectorant, and diaphoretic. Also, these have been used as remedies for hepatitis, arthritis, indigestion, coughs, chronic bronchitis, pleurisy, fever, rheumatism, bacterial and fungal infections, and diseases of the urinary organs. Several classes of compounds such as coumarins, acetylenic compounds, chalcones, sesquiterpenes, and polysaccharides have been reported from these plants. This chapter outlines the results of various scientific studies on Angelica species that were reported to have anticancer and antitumor activities..|