Kyushu University Academic Staff Educational and Research Activities Database
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Yoshizumi Ishino Last modified date:2015.5.20

Graduate School
Undergraduate School
Administration Post

Academic Degree
Doctor of Phamaceutical Science
Field of Specialization
Molecular Biology, Genetic Engineering, Protein Engineering
Research Interests
  • Monitoring of mrine eovironment by metagenomic analysis
    keyword : metagenome, NGS, DNA sequencing, marine
  • Target recognition and expression mechanism of intrinsically disordered protein

    keyword : intrinsically disordered protein, molecular recognition, protein structure
  • Molecular Mechanism of DNA replication
    keyword : DNA replication, molecular mechanism, archaea, hyperthermophiles
  • Molecular mechanisms of DNA recombinatinal repair and genome integrity
    keyword : DNA repair, DNA recombination, endonucelase, homologous recombination, Holliday junction
  • Application of the enzymes and proteins to the development of novel techniques for genetic engineering
    keyword : Genetic Engineering, Gene amplification, Recombinant DNA, NUcleic Acid-relarted enzyme
Current and Past Project
  • Target recognition and expression mechanism of intrinsically disordered protein
Academic Activities
1. Yoshizumi Ishino, Sonoko Iahino,DNA replication in thermophilic microorganisms, In Thermophilic Microorganisms. Horizon Scientific Press, Norwich, UK,Horizon Scientific Press, Norwich, UK,2015.05.
2. Yoshizumi Ishino, Sonoko Iahino,The Mechanisms of DNA Replication
DNA Replication in Archaea, the Third Domain of Life
,INTECH d.o.o., Rijeka, Croatia,pp. 91-126,2013.03.
3. Sonoko Iahino, Yoshizumi Ishino,Thermophilic microbes in environmental and industrial biotechnology
DNA polymerases and DNA ligases
,Springer, 429-457,2013.03.
4. Sonoko Iahino, Yoshizumi Ishino,Microorganisms in Sustainable Agriculture and Biotechnology.
Application of environmental DNA resources to create useful DNA polymerases with different properties.
,Springer,pp. 663-678,2012.03.
1. Yoshizumi Ishino, Sonoko Iahino,DNA polymerases as useful reagents for biotechnology - the history of developmental research in the field. ,2014.08.
2. Yoshizumi Ishino, Sonoko Iahino,The archaeal DNA replication machinery: past, present and future. ,Genes and Genetic Systems,2013.12.
3. Ishino, Y. and Ishino, S.,Rapid progress of DNA replication studies in Archaea, the third domain of life. ,Sci. China, Ser-C Life Sci. ,2012.05.
4. Ishino, S and Ishino, Y.,Application of environmental DNA resources to create useful DNA polymerases with different properties.,Microorganisms in Sustainable Agriculture and Biotechnology. (Springer Science+Business Media),2012.01.
1. Yoshizumi Ishino, Sonoko Iahino, Takeshi YAMAGAMI,A novel endonuclease that may be responsible for damaged DNA base repair in Pyrococcus furious ,Vol.43,No.5,2015.02.
2. Yoshizumi Ishino, Sonoko Iahino,Activation of the MCM helicase from the thermophilic archaeon, Thermoplasma acidophilum by interactions with GINS and Cdc6-2., Springer,Vol.18,2014.07.
3. Yoshizumi Ishino, Sonoko Iahino, Takeshi YAMAGAMI,Multiple interactions of the intrinsically disordered region between the helicase and the nuclease domains of the archaeal Hef protein. ,ASBMB,Vol.289,No.31,2014.06.
4. Kazuo Tori, Sonoko Iahino, Shinichi Kiyonari, Saki Tahara, Yoshizumi Ishino, A novel single-strand specific 3'-5' exonuclease found in the hyperthermophilic archaeon,, Pyrococcus furiosus. ,PLoS ONE,Vol.8,No.3,2013.03.
5. Yumani Kuba, Sonoko Iahino, Takeshi YAMAGAMI, Masahiro Tokuhara, Tamotsu Kanai, Ryosuke Fujikane, Hiromi Daiyasu, Haruyuki Atomi, Yoshizumi Ishino,Comparative analyses of the two PCNAs from the hyperthermophilic archaeon, Thermococcus kodakarensis ,Genes to Cells ,Vol.17,No.11,2012.11.
6. ISHINO Yoshizumi and ISHINO Sonoko,Rapid progress of DNA replication studies in Archaea, the third domain of life.,SCIENCE CHINA  Life Sciences,Vol.55,pp.1-18,2012.05.
7. Ishino, S., Kawamura, A., and Ishino, Y.,Application of PCNA to processive PCR by reducing the stability of its ring structure. ,J. Jap. Soc. Extremophiles. ,Vol.11,No.2,pp. 印刷中,2012.05.
8. Ishino, S., Fujino, S., Tomita, H., Ogino, H., Takao, K., Daiyasu, H., Kanai, T., Atomi, H., and Ishino, Y.,Biochemical and genetical analyses of the three mcm genes from the hyperthermophilic archaeon, Thermococcus kodakarensis. ,Genes to Cells,Vol.16,No.12,pp.1176-1189,2011.11.
9. Oyama T, Ishino S, Fujino S, Ogino H, Shirai T, Mayanagui K, Saito M, Nagasawa N, Ishino Y, Morikawa K.,Architectures of archaeal GINS complexes, essential DNA replication initiation factors,BMC Biol.,Vol.9,pp.28,2011.04.
10. Fujikane, R., Ishino, S., Ishino, Y., Forterre, P.,Genetic analysis of DNA repair in the hyperthermophilic archaeon, Thermococcus kodakaraensis. ,Genes Genet. Syst. ,Vol.85, ,pp.243-257,2010.08.
11. Akita, M., Adachi, A, Takemura, K., Yamagami, T., Matsunaga, F., and Ishino, Y.,Cdc6/Orc1 from Pyrococcus furiosus may act as the origin recognition protein and Mcm helicase recruiter. ,Gens to Cells,Vol.15,No.5,pp.537-552,2010.05.
12. Nishida, H., Mayanagi, K., Kiyonari, S., Sato, Y., Oyama, T., Ishino, Y., and Morikawa, K.,Structural determinant for switching between the polymerase and exonuclease modes in the PCNA-replicative DNA polymerase complex.,Proc. Natl. Acad. Sci. USA. ,Vol.106,No.49,pp.20693-20698,2009.12.
13. Kiyonari, S., Tahara, S., Shirai, T., Iwai, S., Ishino, S., and Ishino, Y.,Biochemical properties and BER complex formation of AP endonuclease from Pyrococcus furiosus,Nucleic Acids Res.,Vol.37,No.19,pp.6439-6453,2009.09.
14. Matsukawa H, Yamagami T, Kawarabayasi Y, Miyashita Y, Takahashi M, Ishino Y.,A useful strategy to construct DNA polymerases with different properties by using genetic resources from environmental DNA,Genes Genet. Syst.,84, 3-13,2009.02.
15. Oyama T, Oka H, Mayanagi K, Shirai T, Matoba K, Fujikane R, Ishino Y, Morikawa K,Atomic structures and functional implications of the archaeal RecQ-like helicase Hjm.,BMC Struct. Biol.,9, 2 (1-12),2009.03.
16. Mayanagi. K., Kiyonari, S., Saito, M., Shirai, T., Ishino, Y., and Morikawa, K.,Mechanism of replication machinery assembly as revealed by the DNA ligase-PCNA-DNA complex architechture.,Proc. Natl. Acad. Sci. USA.,106, 4657-4652 ,2009.03.
17. S. Kiyonari, S. Tahara, M. Uchimura, T. Shirai, S. Ishino, and Yoshizumi Ishino,,Studies on the base excision repair (BER) complex in Pyrococcus furiosus.,Biochem. Soc. Transact.,in press,2008.12.
18. S . Kiyonari, M. Uchimura, T. Shirai, and Yoshizumi Ishino,,Physical and Functional Interactions between Uracil-DNA glycosylase and proliferating cell nuclear antigen from the euryarchaeon Pyrococcus furiosus.,J. Biol. Chem.,283, No35, 24185-24193,2008.08.
19. Yoshimochi, T., Fujikane, R., Kawanami, M., Matsunaga, F., and Ishino, Y.*,The GINS complex from Pyrococcus furiosus stimulates the MCM helicase activity.,Journal of Biological Chemistry,283, No3, 1601-1609,2008.01.
20. Tori, K., Kimizu, M., Ishino, S., and Ishino. Y. ,Both DNA polymerase BI and D from the hyperthermophilic archaeon, Pyrococcus furiosus bind to PCNA at the C-terminal PIP box motifs. ,Journal of Bacteriology,189, 5652-5657,2007.05.
21. Kiyonari, S., Kamigochi, T., and Ishino, Y. ,A single amino acid substitution in the DNA-binding domain of Aeropyrum pernix DNA ligase impairs its interaction with proliferating cell nuclear antigen,Extremophiles,11, 675-684,2007.05.
22. Matsunaga, F., Glatigny, A., Mucchielli-Giorgi, M. H., Agier, N., Delacroix, H., Marisa, M., Durosay, P., Ishino, Y., Aggerbeck, L., and Forterre, P. ,Genomewide and Biochemical Analyses of DNA-binding activity of Cdc6/Orc1 and Mcm proteins in Pyrococcus sp. ,Nucleic Acids Res. ,35, 3214-3222.,2007.04.
23. Imamura, K., Fukunaga, K., Kawarabayasi, Y., and Ishino, Y.,Specific interactions of three PCNAs with replication-related proteins in Aeropyrum pernix.,Molecular Microbiology ,64, 308-318,2007.01.
24. Kiyonari, S., Takayama, K., Nishida, H., Ishino, Y.,Identification of a novel binding motif in Pyrococcus furiosus DNA ligase for the functional interaction with proliferating cell nuclear antigen.,J. Biol. Chem.,281 巻 28023-28032ページ,2006.07.
25. Miyata, T., Suzuki, H., Oyama, T., Mayanagi, K., Ishino, Y., and Morikawa, K.,Open clamp structure in the clamp-loading complex visualized by electron microscopic image analysis.,Proc. Natl. Acad. Sci. USA,Vol.102,No.39,102, 13795-13800,2005.09.
26. Fujikane, R., Komori, K., Shinagawa, H., and Ishino, Y.,Identification of a novel helicase activity unwinding branched DNAs from the hyperthermophilic archaeon, Pyrococcus furiosus.,J. Biol. Chem.,Vol.280,No.13,280, 12351-12358.,2005.04.
1. Ishino Y. (2008) Contribution of the proteins from hyperthermophilic archaea to structural and functional analyses of DNA replication/repair apparatus. Keynote Presentation at Extremophiles 2008 Sept. 7-11, Cape Town, South Africa..