Kyushu University Academic Staff Educational and Research Activities Database
List of Presentations
Tetsuo Kondo Last modified date:2019.08.13

Professor / Division of Sustainable Bioresources Science / Department of Agro-environmental Sciences / Faculty of Agriculture


Presentations
1. Tetsuo Kondo, Process Design Due to Nano-Fusion for Reinforced Nanocomposites by Embedding Nanocellulose Honeycomb Frames, 2019 International Conference of Nanotechnology for Renewable Materials (TAPPI Nano 2019), 2019.06.
2. Shingo Yokota, Koki Miura, Tetsuo Kondo, Oriented deposition of bacterial nanocellulose induced by nematic ordered cellulose templates with unique surface energy distribution, 257th ACS National Meeting, 2019.04, Ordered deposition of nanocelluloses on the nematic ordered cellulose (NOC) was investigated in the viewpoint of the surface energy distribution of substrates. NOC is a unique template that induces oriented deposition of other objects due to the alternately aligned amphiphilic molecular tracks on its surface. To chenge the surface energy, styrene oligomers were introduced onto the cellulose molecular tracks on the surface of NOC by surface-initiated atom transfer radical polymerization. The introduced hydrophobic moieties were partly converted into hydrophilic by the following UV-irradiation. Surface energy of the resultant templates was quantitatively estimated based on contact angle measurements. Gluconacetobacter xylinus (G. xylinus) cells were then cultivated on the more hydrophilic NOC template above prepared. G. xylinus secreted bacterial cellulose (BC) that was deposited parallel to the oriented molecular tracks on the substrate similar to the original NOC, resulting in a linear movement pattern of the bacteria. However, decrease in the moving rate of G. xylinus and the morphology change of deposited BC indicated less attractive interaction engagements onto the more hydrophilic NOC templates comparing to the original NOC. This suggests that oriented deposition of BC on the NOC is presumably induced by strong hydrophobic interfacial interaction..
3. Tetsuo Kondo, Gento Ishikawa, Filed surface coating for PET with bamboo-ACC nanocellulose to allow to find a suitable container for the resin-adsorbable nanocellulose, 257th ACS National Meeting, 2019.04, The authors proposed Aqueous counter collision (ACC) process, which is a gentle and rapid method, to prepare separate cellulose nanofibers (ACC-nanocellulose) as dispersion in water using only a pair of water jets, without the need for any chemical modifications. Although the surface of nanocellulose has been widely presumed as highly hydrophilic, the ACC process has shown the capability to impart hydrophobic faces onto the nanocellulose surfaces, resulting in amphiphilic Janus faces.
The unique amphiphilic surface properties of non-modified ACC-nanocellulose, exhibited adsorption capability to surfaces on hydrophobic resins such as polypropylene (PP). Therefore, this paper attempts to clarify availability of adsorption for the amphiphilic Janus ACC-nanocellulose onto various surfaces of widely used resins. Not only PP particles but other surfaces were expected to be applicable for the adsorption of ACC-nanocellulose. However, the result exhibited that the adsorption was better for PP rather than other plastic resins, and moreover the ability was found more or less for any plastic resin except PET. In fact, the surface on PET particles indicated almost no adsorption with ACC-nanocellulose. Reversely taking this into consideration, we could find a suitable container for our resin- adsorbable nanocellulose..
4. Tetsuo Kondo, Shingo Yokota, Eiko Megan Uchida, Gento Ishikawa, Satomi Tagawa, Masato Kamogawa, Design for reinforced nanocomposites having embedded bamboo-ACC nanocellulose honeycomb by fabrication process due to nano-fusion, 257th ACS National Meeting, 2019.04, The authors proposed Aqueous counter collision (ACC) process, which is a gentle and rapid method, to prepare separate cellulose nanofibers (ACC-nanocellulose) as dispersion in water using only a pair of water jets, without the need for any chemical modifications. Although the surface of nanocellulose has been widely presumed as highly hydrophilic, the ACC process has shown the capability to impart hydrophobic faces onto the nanocellulose surfaces, resulting in amphiphilic Janus faces.
The ACC-nanocellulose in fact exhibited different surface characteristics from those for other nanofibers prepared in different manners; particularly it appeared in stability of their emulsion, adsorption to hydrophobic resins and others, due to the hydrophobic faces on the ACC-nanocellulose surface.
This study involved how such some unique amphiphilic surface properties of the non-modified nanocellulose prepared by ACC can be applied to fabricate nanocomposites directly with polypropylene (PP) without thermal mixing/excluding. The critical process for it is to minimally coat the PP particle as a starting material with ACC-nanocellulose simply by immersing in the diluted aqueous suspension. The coated surface on PP particles are supposed to exhibit the melting point depression due to the interaction between the two components, whereas the cores of the PP are still intact. The DSC curves indicated appearance of the surface melting and then the core melting as heating temperature increased. Therefore, preheating with pressing at the temperature for the surface melting induced fusion among the coating ACC-nanocellulose to form cross-linking, leading to honeycomb frames..
5. Suresh Rao. N, @近藤 哲男, A nano-architecture on polysacchride scaffold using ACC biotic nanofibers for skin engineering application, 第33回繊維学会西部支部 講演会・見学会, 2018.12.
6. @Tetsuo Kondo, Genetically modified bacterial nano-machines for in vivo producing cellulose bio-nanocomposites, International Conference on Pulping, Papermaking and Biotechnology 2018(ICPPB’18), 2018.11, 基調講演:International Conference on Pulping, Papermaking and Biotechnology 2018(ICPPB’18)、中国・南京市で開催.
7. Suresh Rao, 近藤 哲男, Double phase biopolysacchride scaffolds coated with ACC biotic nanofibers for skin engineering application, セルロース学会第25回年次大会, 2018.07.
8. Tetsuo Kondo, Preparation of Polypropylene Nanocomposites with Amphiphilic Janus ACC-Nanocellulose Created by Aqueous Counter Collision, 2018 International Conference on Nanotechnology for Renewable Materials(TAPPI), 2018.06, 2018 TAPPI International Conference on Nanotechnology for Renewable Materials:アメリカ・マディソンで開催.
9. Takeru Ishihara, Daisuke Tatsumi, Tetsuo Kondo, Characterization of cellulose/cellulose acetate films prepared by coagulation method of blended ionic liquid solution, The 4th International Cellulose Conference(ICC2017), 2017.10.
10. Yuri Uchi, Satomi Tagawa, Tetsuo Kondo, Semi-artificial system producing β-1,3-glucan micro-hollow fiber by fixing a single plant cell into micro channel flowing device, The 4th International Cellulose Conference(ICC2017), 2017.10.
11. Kunio Tsuboi, Tsubasa Tsuji, Tetsuo Kondo, Mineralization Process In Preparation Of Cellulose Nanofiber-Calcium Carbonate Nanocomposites Produced By The On-site Aqueous Counter Collision Method, The 4th International Cellulose Conference(ICC2017), 2017.10.
12. Hikari Utsunomiya, Tetsuo Kondo, “Cellulose nano-anemone” as a Janus nanofiber having nano-tentacles, The 4th International Cellulose Conference(ICC2017), 2017.10.
13. Shingo Yokota, Koki Miura, Tetsuo Kondo, Oriented deposition of nanocellulose secreted from Gluconacetobacter xylinus induced by nematic ordered cellulose templates with unique surface energy distribution, The 4th International Cellulose Conference(ICC2017), 2017.10.
14. Yukako Hishikawa, Eiji Togawa, Tetsuo Kondo, Characterization of hydrogen bonds in cellulose II crystals using polarized FTIR accompanied with vapor-phase deuteration, The 4th International Cellulose Conference(ICC2017), 2017.10.
15. Natsumi Kitazaki, Momoko Kitamikado, Daisuke Tatsumi, Tetsuo Kondo, Viscoelastic Properties of Cellulose Gels Having “Macroscopic Hierarchical Patterns”, The 4th International Cellulose Conference(ICC2017), 2017.10.
16. Misa Miyazaki, Daisuke Tatsumi, Tetsuo Kondo, Long periodic structure of mercerized cellulose using X-ray and light scattering measurements, The 4th International Cellulose Conference(ICC2017), 2017.10.
17. Kohei Yamashita, Yuka Koga, Daisuke Tatsumi, Tetsuo Kondo, Preparation of optical anisotropic gels from chitin and cellulose, The 4th International Cellulose Conference(ICC2017), 2017.10.
18. Gento Ishikawa, Tetsuo Kondo, Dual Nano-size Effects of ACC-nanocellulose Characterized by Poly(vinyl alcohol) Crystallization Behavior as A Probe, The 4th International Cellulose Conference(ICC2017), 2017.10.
19. Aki Sugiyama, Tetsuo Kondo, Unique properties of “Green emulsion” using ACC-nanocellulose, The 4th International Cellulose Conference(ICC2017), 2017.10.
20. Eiko Megan Uchida, Shingo Yokota, Tetsuo Kondo, Novel nanocomposites prepared from polypropylene micro-particles coated with amphiphilic ACC bamboo nanocellulose, The 4th International Cellulose Conference(ICC2017), 2017.10.
21. Suresh Rao, Tetsuo Kondo, Nematically ordered Polysaccharide scaffold coated with ACC treated cellulose nanofibers for skin tissue engineering applications, The 4th International Cellulose Conference(ICC2017), 2017.10.
22. Satomi Tagawa, Tetsuo Kondo, Visualization of cellulose deposition onto surfaces of plasma membranes in plant protoplasts during primary cell wall formation, The 4th International Cellulose Conference(ICC2017), 2017.10.
23. Ryo Takahama, Tetsuo Kondo, Characterization of Polysaccharide Nanocomposites in vivo Synthesized by Transgenic Gluconacetobacter xylinus, The 3rd Symposium of Bacterial NanoCellulose(BNC2017), 2017.10.
24. Suresh Rao, Tetsuo Kondo, Nematically ordered Polysaccharide scaffold coated with ACC treated cellulose nanofibers for skin tissue engineering applications, The 3rd Symposium of Bacterial NanoCellulose(BNC2017), 2017.10.
25. Shingo Yokota, Keita Kamada, Mariko Ago, Orlando J Rojas, Tetsuo Kondo, Surface active nanocellulose prepared by the aqueous counter collision method, EPNOE2017 5th EPNOE International Polysaccharide Conference, 2017.08.
26. Hikari Utsunomiya, Tetsuo Kondo, “Cellulose nano-anemone” having fibrillated reducing ends as an anisotropic cellulose nanofiber fabricated by the aqueous counter collision, EPNOE2017 5th EPNOE International Polysaccharide Conference, 2017.08.
27. Aki Sugiyama, Tetsuo Kondo, Amphiphilic ACC-nanocellulose pickering emulsion as a template for fabrication of three dimensional hierarchical structures, EPNOE2017 5th EPNOE International Polysaccharide Conference, 2017.08.
28. Eiko Megan Uchida, Tetsuo Kondo, Novel polypropylene composites with non-modified but amphiphilic bamboo nanocellulose, EPNOE2017 5th EPNOE International Polysaccharide Conference, 2017.08.
29. Satomi Tagawa, Yusuke Yamagishi, Ugai Watanabe, Ryo Funada, Tetsuo Kondo, Formation of β-1,3-glucan hollow fiber from plant protoplasts in response to intracellular and extracellular environmental stimuli, EPNOE2017 5th EPNOE International Polysaccharide Conference, 2017.08.
30. Tetsuo Kondo, Polysaccharide nanotechnology using bio-alchemy and water, EPNOE2017 5th EPNOE International Polysaccharide Conference, 2017.08, 基調講演:5th EPNOE International Polysaccharide Conference (欧州多糖類国際会議2017:ドイツ・イエナで開催 )(2017/8/20~8/24)
「Polysaccharide Nanotechnology Using Bio-alchemy and Water」という題目で、水と生物機能を用いる近藤独自のナノテクノロジーについて基調講演を行った。.
31. Suresh Rao.N, 近藤 哲男, Feasibility of nematically ordered polysaccharide templates for skin tissue engineering application, 第54回化学関連支部合同九州大会, 2017.07.
32. Tetsuo Kondo, Shingo Yokota, Eiko Megan Uchida, Feasible Application of Hydrophobicity in Amphiphilic ACC-Nanocellulose Created by Aqueous Counter Collision (ACC), TAPPI's 2017 International Conference on Nanotechnology for Renewable Materials, 2017.06.
33. Shingo Yokota, Keita Kamada, Mariko Ago, Orland J Rojas, Tetsuo Kondo, Emulsification Behavior of Amphiphilic Nanocellulose Prepared by Aqueous Counter Collision, TAPPI's 2017 International Conference on Nanotechnology for Renewable Materials, 2017.06.
34. Tetsuo Kondo, Satomi Tagawa, Visualization of dynamic changing in formation of cell wall cellulose and callose along with arrangements of microtubules with GFP on surfaces of protoplast cells, 253th ACS National Meeting, 2017.04.
35. Tetsuo Kondo, LB film preparation of regioselectively substituted cellulose cinnamates on nematic ordered cellulose templates
, 253th ACS National Meeting, 2017.04.
36. Tetsuo Kondo, Kunio Tsuboi, Shingo Yokota, Determination of hydrophobicity in amphiphilic nanocellulose imparted by Aqueous Counter Collision (ACC), 253th ACS National Meeting, 2017.04.
37. Shingo Yokota, Keita Kamada, Tetsuo Kondo, Pickering emulsion stabilized using amphiphilic ACC-nanocellulose, PFI(Paper and Fibre Research Institute), 2016.10, Stable Pickering emulsion was successfully prepared by using amphiphilic cellulose nanofibers obtained by the aqueous counter collision (= ACC) method using dual water-jets. In the ACC process, water-dispersed single nanofibers (ACC-nanocellulose, (= ACC-Nac)) was prepared through selective cleavage of van der Waals forces in native crystalline cellulose, which would trigger the exposure of the inherent hydrophobic face on the surface of ACC-Nacs. In this study, we investigated regarding ACC-Nacs as an emulsifier. Water dispersions of the ACC-Nac derived from wood-related microcrystalline cellulose were ultrasonically mixed with non-polar solvents. As a result, oil-in-water Pickering emulsions were reproducibly prepared, whereas the O/W emulsion state was negligibly changed at room temperature for a long-time. Besides, the stability of Pickering emulsion from ACC-Nacs was dependent on the permittivity of non-polar solvents. Such an inducing ability of Pickering emulsion with the ACC-Nac was significantly unique and higher than hydrophilic TEMPO-oxidized cellulose nanofibers, which indicates the unique amphiphilic surface properties of ACC-Nacs..
38. 近藤 哲男, “Bio-alchemy Using Water and Biological Systems”ーThree-dimensional biofabrication on ordered cellulose templates and ACC-nanodecompositionー, Inha University-seminar, 2016.07.
39. @近藤 哲男, Pure single cellulose nanofibers of amphiphilic properties with hydrophobic surfaces created by aqueous counter collision, 2016 TAPPI International Conference on Nanotechnology for Renewable Materials, 2016.06.
40. 近藤 哲男, Which was the first to appear, β-1,4 or β-1,3 glucans?, 251th ACS National Meeting, 2016.03.
41. 横田 慎吾, 西元 愛里, 近藤 哲男, Surface activation of ACC-nanocellulose for chemical modification in an aqueous system, 251th ACS National Meeting, 2016.03.
42. 菱川 裕香子, 近藤 哲男, Analyses on hydrogen bonding in noncrystalline regions of regioselectively methylated cellulose films by a combination of vapor-phase deuteration and generalized two-dimensional correlation IR spectroscopy, 251th ACS National Meeting, 2016.03.
43. 横田 慎吾, 近藤 哲男, Chemical modification of cellulose nanofibers via surface activation in an aqueous dispersion system, EMN Meeting on Cellulose (Energy Materials Nanotechnology), 2016.03.
44. 近藤 哲男, Brief introduction on Nanocellulose researches in Japan, 日本・カナダナノセルロース国際シンポジウム, 2016.01.
45. 鎌田 啓大, 横田 慎吾, 近藤 哲男, Pickering emulsion stabilized using amphiphilic cellulose nanofibers prepared by the aqueous counter collision method, The 2015 International Chemical Congress of Pacific Basin Societies(PACIFICHEM 2015), 2015.12, This study deals with the amphiphilic surface characteristics of the cellulose nanofibers prepared by the aqueous counter collision (=ACC) method. The ACC method allows bio-based materials to pulverize into nano-objects using dual high-speed water jets. Intermolecular interactions of bio-based materials are cleaved selectively depending on the ejecting pressure. By the ACC-treatment of native cellulose fibers, water-dispersed single nanofibers are obtained, which is termed as “ACC-nanocellulose”. In the crystallization step of cellulose, glucan chains associate to form glucan sheets via hydrogen bonds to establish a situation that glucan sheets are stacked through van der Waals forces between hydrophobic planes. Kinetic energy of ACC is theoretically capable of selective cleavage of van der Waals forces in cellulose fibers, resulting in the exposure of the hydrophobic surface of ACC-nanocellulose.
In this study, we investigated the emulsifying behaviors of the aqueous dispersion of thus prepared ACC-nanocellulose with non-polar solvents in order to demonstrate the apparent amphiphilicity. When aqueous dispersions of ACC-nanocellulose derived from wood were ultrasonically mixed with non-polar solvent, Pickering emulsion was formed at the interface between the aqueous phase containing ACC-nanocellulose and non-polar solvent phase. In particular, a stable emulsion was formed using cyclohexane in comparison to n-hexane, toluene, and ethyl acetate. Such Pickering emulsion forming ability of ACC-nanocellulose was significantly unique and higher than hydrophilic TEMPO-oxidized cellulose nanofibers, which strongly indicates the amphiphilic surface properties of ACC-nanocellulose.
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46. 田川 聡美, 山岸 祐介, 渡邊 宇外, 船田 良, 近藤 哲男, Production of a β-1,3-glucan hollow fiber due to an environmental stress response in plant protoplasts , The 2015 International Chemical Congress of Pacific Basin Societies(PACIFICHEM 2015), 2015.12, In our previous report, it was found that under a Ca2+-rich and acidic condition, protoplasts isolated from white birch (Betula platyphylla) leaves calluses produced a bundle of hollow fibrils consisted of β-1,3-glucan (a callose hollow fiber) due to an environmental stress response.
In this unique phenomenon, cortical microtubules (CMTs) were predicted to contribute to formation of the above structure, because the distributions of callose synthases in plasma membrane may be controlled by CMTs as previously reported. Therefore, we investigated changes of CMTs of protoplasts in producing a callose hollow fiber. To visualize CMTs, the mammalian microtubule-associated protein 4 with the green fluorescent protein (GFP-MAP4) gene was introduced into the calluses. When protoplasts prepared from the genetically-modified calluses were cultured under the above stress condition, GFP-MAP4-labeled CMTs surrounded the producing site of a callose hollow fiber in a random manner. To monitor the formation process of a callose hollow fiber, polymerization or de-polymerization of CMTs was inhibited by using oryzalin or paclitaxel, respectively. In the case of paclitaxel, the fiber width became larger when compared with those in a stress condition without inhibitors. In contrast for the case of oryzalin, the fiber width became thinner. These results indicate that changing of CMTs possibly affects the production quantity or self-assembly formation of callose hollow fibrils. This study would provide an understanding why the three-dimensional hollow structure of a biological polysaccharide was formed.
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47. 辻田 裕太郎, 近藤 哲男, Nano-pulverization of collagen fibrils by aqueous counter collision with assistance of activated water, 2015 Pusan-Gyeongnam/Kyushu-Seibu Joint Symposium on High Polymers (17th) and Fibers (15th), 2015.11.
48. 宇都宮 ひかり, 近藤 哲男, "Cellulose nano-anemone” prepared by aqueous counter collision of bacterial nanocellullose, 2nd International Symposium on Bacterial Nanocellulose GDANSK 2015, 2015.09.
49. 近藤 哲男, Bacterial cellulose architecture from "molecules" via nano to "3D-materials", 2nd International Symposium on Bacterial Nanocellulose GDANSK 2015, 2015.09.
50. Ju Fang, 河野 信, 田島 健次, 近藤 哲男, Celllulose/Curdlan Nanocomposites from Gene-engineered Gluconacetobacter xylinus
, セルロース学会第22回年次大会, 2015.07.
51. 近藤 哲男, Fabrication of "Cellulose Nano-Anemone", 2015 TAPPI International Conference on Nanotechnology for Renewable nanomaterials, 2015.06.
52. Yukako Hishikawa, Tetsuo Kondo, Characterization of noncrystalline regions in regioselectively methylated cellulosic films using vapor-phase deuteration and generalized 2D correlation infrared spectroscopy, 249th ACS National Meeting, 2015.03.
53. Tetsuo Kondo, Fabrication and Characterization of cellulose nanoanemone, 249th ACS National Meeting, 2015.03.
54. Shingo Yokota, Tetsuo Kondo, Surface Reactivity in an Aqueous System of Bio-Nanofibers Prepared by the Aqueous Counter Collision Method
, International Symposium on Fiber Science and Technology 2014 (ISF2014), 2014.09.
55. Yohei Kawano, Tetsuo Kondo, Preparation of Aqueous Dispersions of Multi-Walled Carbon Nanotubes by the Aqueous Counter Collision Method
, International Symposium on Fiber Science and Technology 2014 (ISF2014), 2014.09.
56. Hikari Utsunomiya, Shingo Yokota, Tetsuo Kondo, Preferential Subfibrillation from Reducing Ends in the Initial Stage of Nano-Pulverization Using Aqueous Counter Collision
, International Symposium on Fiber Science and Technology 2014 (ISF2014), 2014.09.
57. Yutaro Tsujita, Shingo Yokota, Tetsuo Kondo, Self-Assembling Behaviors of Collagen Nanofibers in the Aqueous Dispersions Prepared by the Aqueous Counter Collision Method
, International Symposium on Fiber Science and Technology 2014 (ISF2014), 2014.09.
58. Chemical Reactivity on Surface of Cellulose Nanofibers Prepared by the Aqueous Counter Collision Method
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59. 方 駒, 近藤 哲男, 田島 健次, 河野 信, 形質転換された酢酸菌より分泌されるカードラン/セルロース複合体, セルロース学会第21回年次大会, 2014.07, Gluconacetobacter xylinus is well known for its remarkable ability to secrect cellulose nanofiber extracellularly. By modifying this natural nanofiber producing system, it’s possible to obtain other kinds of polysaccharide materials or composites. In this study, a novel curdlan/cellulose composite was successfully obtained by transforming a curdlan synthase gene from Agrobacterium sp. to Gluconacetobacter xylinus. The results suggested that the curdlan might be intracellularly synthesized then secreted accompanied with the formed cellulose nanofibers, resulted in the formation of a curdlan-covered pellicle. This study explored a potential approach to obtain polysaccharide composites through biosynthesis..
60. Different Nano-pulverizing Behaviors in the Aqueous Counter Collision Process of Microbial Cellulose Fibers Secreted by Gluconacetobacter xylinus under Different Culture Conditions
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61. Change of cell polarity in secreting β-1,3-glucan hollow fibrils of callus protoplasts under a stress culture.
62. Nanocomposite of Cellulose Nanofiber and CaCO3 Prepared by the On-site Aqueous Counter Collision Method.
63. Preparation of Thinner Cellulose Nanofibers Using the Modified Aqueous Counter Collision System with a Novel Additive.
64. Yukako Hishikawa, Tetsuo Kondo, Characterization of noncrystalline regions of cellulose derivatives using vapor-phase deuteration and generalized 2D correlation infrared spectroscopy, 247th ACS National Meeting, 2014.03.
65. Tetsuo Kondo, Gento Ishikawa, Nanosize dependence of fiber width on interfacial interactions in cellulose nanocomposites with poly(vinyl alcohol), 247th ACS National Meeting, 2014.03.
66. Yutaro Tsujita, Shingo Yokota, Tetsuo Kondo, Collagen nanofibers as a novel building block prepared by the aqueous counter collision method, 247th ACS National Meeting, 2014.03.
67. Yohei Kawano, Tetsuo Kondo, Induced water dispersibility of the multi-walled carbon nanotubes by the aqueous counter collision method, 2013 Kyushu-Seibu/Pusan-Gyeonnam Joint Symposium on High Polymers(16th) and Fibers(14th), 2013.11.
68. Kunio Tsuboi, Shingo Yokota, Tetsuo Kondo, Difference between bamboo- and wood-derived cellulose nanofibers prepared by the aqueous counter collision method, 2013 Kyushu-Seibu/Pusan-Gyeonnam Joint Symposium on High Polymers(16th) and Fibers(14th), 2013.11.
69. Liwei Yu, Daisuke TATSUMI, Tetsuo Kondo, Preparation of nano carbon particles from activated carbon using the aqueous counter collision treatment, 2013 Kyushu-Seibu/Pusan-Gyeonnam Joint Symposium on High Polymers(16th) and Fibers(14th), 2013.11.
70. Kazuya Fujiwara, Daisuke TATSUMI, Tetsuo Kondo, Single relaxation behavior in dynamic viscoelastic properties of green seaweed polysaccharide ulvan/AlCl3 aqueous systems, 2013 Kyushu-Seibu/Pusan-Gyeonnam Joint Symposium on High Polymers(16th) and Fibers(14th), 2013.11.
71. Shingo Yokota, Shiro Sakoda, Tetsuo Kondo, Interfacial Design of Nano-sized and Nano-structured Cellulose Materials by Chemical Modification, EPNOE2013 3rd EPNOE International Polysaccharide Conference, 2013.10.
72. Ju Fang, Satoshi Nakagawa, Shin Kawano, Kenji Tajima, Tetsuo Kondo, Secretion of cellulose / curdlan nanocomposites by gene-transformed Gluconacetobacter xylinus, EPNOE2013 3rd EPNOE International Polysaccharide Conference, 2013.10.
73. Saki Nagamoto, Tetsuya Takahashi, Shingo Yokota, Tetsuo Kondo, Polysaccharide nanofibers secreted by the pink snow mold fungus in Antarctica depending on temperature stress, EPNOE2013 3rd EPNOE International Polysaccharide Conference, 2013.10.
74. Hikari Utsunomiya, Shingo Yokota, Tetsuo Kondo, Preferential cleavage of reducing ends in cellulose fibers for nano-pulverization using aqueous counter collision, EPNOE2013 3rd EPNOE International Polysaccharide Conference, 2013.10.
75. Takahiro Kojima, Shingo Yokota, Tetsuo Kondo, Ordered biomineralization mediated by a host-guest reaction on unique oriented polysaccharide templates, EPNOE2013 3rd EPNOE International Polysaccharide Conference, 2013.10.
76. Tetsuo Kondo, Tomoko Seyama, Three-dimensional biofabrication on nematic ordered cellulose templates, EPNOE2013 3rd EPNOE International Polysaccharide Conference, 2013.10.
77. 方 駒, 中川 理, 近藤 哲男, 田島 健次, 河野 信, アグロバクテリウム由来のカードラン合成遺伝子の酢酸菌への導入の試み
, セルロース学会第20回年次大会, 2013.07, Gluconacetobacter xylinus, a representative cellulose production bacterium, is well-known for its 3-D cellulose fiber secreting ability. In this work, an attempt of introduction of the (1→3)-β-glucan (curdlan) synthesizing ability to Gluconacetobacter xylinus has been carried out by transforming the curdlan synthesizing gene from Agrobacterium sp. ATCC 31749 to Gluconacetobacter xylinus ATCC 23769. The successful construction of the expression vector was confirmed by electroporation and sequence detection. The results showed that the modified G. xylinus lost an ability to form pellicle but secreted polysaccharides-like compounds dispersed in the medium. The composition and structure of the secretion is now under investigation. .
78. 方 駒, 中川 理, 河野 信, 田島 健次, 近藤 哲男, 遺伝子組換えした酢酸菌によるカードランナノコンポジットファイバーの生産, 第50回化学関連支部合同九州大会, 2013.07, Gluconacetobacter xylinus(G. xylinus) is widely studied because of its excellent 3-D cellulose fiber properties. In this work, we tried to introduce a(1→3)-β-glucan(curdlan) synthesizing ability to G. xylinus by transforming the curdlan synthesizing gene (crdS) from Agrobacterium sp. in order to allow G. xylinus to have a multiple fiber forming capacity to produce a novel curdlan-containing composite nanofiber..
79. Tetsuo Kondo, Gento Ishikawa, Nano-size effct of cellulose fibers in interfacial interactions for nano-composites with poly(vinyl alcohol), The 17th International Symposium on Wood,Fiber and Pulping Chemistry, 2013.06.
80. Shingo Yokota, Tetsuo Kondo, Surface acetylation of cellulose-based nanofibers prepared by aqueous counter collision, The 17th International Symposium on Wood,Fiber and Pulping Chemistry, 2013.06.
81. Tetsuo Kondo, Fabrication of a uniaxially oriented nano-fibrous film by drawing of microbial cellulose pellicle secreted by Gluconacetobacter xylinus under an oxygen–lacking environment, 245th ACS National Meeting and Exposition, 2013.04, A drawable microbial cellulose pellicle having a minimum physical cross-linkage of the nanofibers was secreted by Gluconacetobacter xylinus cultured in a closed space of Schramm-Hestrin culture medium covered with silicone oil for preventing immediate use of the ambient oxygen gas. The crystalline structure of the fibers thus obtained was more than 90 % rich in cellulose I crystalline phase, which the normal culture had not provided to date. Moreover, the obtained pellicle allowed stretching at 1.5 times to provide a novel film with oriented crystalline nanofibers. The mechanical properties and thermal stability exhibited superior to widely used polymeric materials. It was also noted that the heating process induced transformation of the dominant cellulose I crystalline phase into I phase without a loss of the crystallinity and the high Young’s modulus. The microbial culture under an oxygen-lacking stress could offer fabrication of a novel oriented nano-fibrous film of cellulose I promising excellent potential properties. .
82. Interfacial design of cellulose nano-objects by chemical modification.
83. Surface acetylation of cellulose nanofibers prepared by aqueous counter collision.
84. Novel ordered cellulose templates mediating host-guest biomineralization.
85. Preparation of three-dimensional architecture by living radical polymerization initiated from nematic ordered cellulose
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86. Preparation of the water-dispersible carbon nanotube using aqueous counter collision.
87. Gelation behavior of Konjac-glucomannan with different dispersibility.
88. Preparation of oriented films using a novel cellulose nanofiber gel
secreted by Gluconacetobacter xylinus.
89. Surface modification of cellulose nanofibers prepared by aqueous counter collision.
90. Attempt for preparation of conductive cellulose seats using fine graphite particles.
91. An attempt to introduce a fiber-secreting ability of (1→3)-β-glucan
to Gluconacetobacter xylinus.
92. Nanofibres prepared from bio-based materials using aqueous counter collision.
93. “Biomimic-mineralization" mediated by a bifunctional cellulose template.
94. Surface modification in an aqueous system of biobased nanofibers prepared by counter collision.
95. Characterization of single cellulose nanofibers prepared by the aqueous counter collision of pellicles secreted by Acetobacter xylinum.
96. Size Dependence of Cellulose Nanofibers on The Interfacial Interaction with Poly(Vinyl Alcohol) Molecules in The Composites.
97. Aqueous counter collision as a novel tool to hierarchically and rapidly decompose a cellulose fiber into the single nanofibers.
98. Morphology of single "nanocellulose" prepared from the pellicle of Acetobacter xylinum using aqueous counter collision

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99. Influential factors to enhance the moving rate of Acetobacter xylinum due to its nanofiber secretion on oriented templates
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100. Mineralization of hydroxyapatite on nematic ordered cellulose templates.
101. Investigation of molecular orientation in nematic ordered cellulose films with vapor-phase deuteration and polarized FTIR
.
102. Patterning in movements and deposition of secreted cellulose nanofiber of Acetobacter xylinum directed by an interfacial interaction on ordered chitin templates.
103. Regulated movements and cellulose nanofiber deposition of Acetobacter Xylinum on polysaccharide templates as a scaffold .
104. The morphology of cellulose nanofibers obtained using the aqueous counter collisionates.
105. Synthesis,properties and their LB film preparation of regioselectively substituted cellulose cinnamates .
106. Synthesis,properties and their LB film preparation of regioselectively substituted cellulose cinnamates .
107. Inducing orientation of building blocks on the ordered cellulose templates.
108. Attempts for fabrication of an artificial cell wall.
109. Hierarchical organizing design for 3-D architecture of cellulose as a building block.
110. Structure of honeycomb-patterned film from polysaccharides using W/O emalsion.
111. Nanofabrication of orientated inorganic-organic layered composites.
112. Characterization of noncrystalline regions in cellulose using a FTIR deuteration monitoring and generalized two-dimensional correlation spectroscopy.
113. Concentration Dependence of the Reaction Mechanism of Hydroxymethylphenols in Alkaline Medium.
114. An Artificial Cell Wall.
115. Recent Advances in Cellulose Science -Potential of Bio-Nanofibers Secreted by a Bacterium-.
116. A hierarchical organizing design for 3-D architecture using a bacterium extruding a cellulose nanofiber.
117. Fabrication of honeycomb-patterned carbohydrate polymer films.
118. A new approach for nano-assembly of orientated imogolite-cellulose layered composites.
119. Fabrication of honeycomb-patterned carbohydrate polymer films.
120. A hierarchical organizing design for 3-D architecture using a bacterium extruding a cellulose nanofiber.
121. A hierarchical organizing design for 3-D architecture using a bacterium extruding a cellulose nanofiber.
122. Autofabrication of 3D structure on honeycomb-patterened cellulose film as a scaffold by Acetobacter xylinum.
123. Auto nano-fabrication of 3D structure using Acetobacter xylinum.
124. A hierarchical structure of a gigantic callose fiber secreted from Betula protoplasts.
125. Ordered characteristic surfaces with various scales prepared by deposition of callose fiberes onto the nematic ordered cellulose substrate.
126. Hierarchical structure of a gigantic callose fiber secreted from a protoplast.
127. Bottom−up auto-nanofabrication of 3-dimentional structures by regulated movements and fiber-seceretion of bacteria on the scaffold.
128. Fabrication and Characterization of Honeycomb-Patterened Cellulose.
129. Uniaxial ordering of hyaluronan molecular assembly on a nematic ordered cellulose template.
130. Synthesis and Properties of Regioselectively Substituted Cellulose Cinnamates.
131. Structure of the polysaccharide produced by the snow mold fungus,Microdochium nivale.
132. A suitable medium for the deposition of nanofibrils of bacteria on the nematic ordered cellulose template.
133. Structural characteristics of simultaneous-biaxial drawn cellulose film prepared from water-swollen cellulose.
134. Localization of acsC and D proteins in Acetobacter xylinum revealed by immunocytochemistry.
135. Function of ORF2 gene in cellulose biosynthesis of Acetobacter xylinum.
136. Fabrication and characterization of honeycomb-patterned film from cellulose and celluose/chitosan blends.
137. Nanotechnology for development of functionalized biomaterials from wood.
138. Properties of the cellulose/imogolite nanocomposite.
139. Synthesis and properties of regioselectively substituted cyanoethylcellulose.
140. Reduction of VOC in houses constructed by WB building method.
141. Dynamic analysis on formation of wood cell wall structure.
142. Gigantic callose fiber secreted from a protoplast.
143. The structure of callose fiber produced from protoplast.
144. Structural characteristics and mechanical properties of biaxially drawn cellulose films prepared from water-swollen cellulose.
145. Chracterization of the noncrystalline regions of cellulose derivatives using generalized two-dimensional correlation spectroscopy.
146. Structure of monolayers from cellulose derivatives.
147. Deposition of secreted cellulose fibers and movements of Acetobacter xylinum on nematic ordered chitin template.
148. Analysis of the noncrystalline regions of cellulose using generalized two- dimensional correlation spectroscopy.
149. Two-Dimensional Molecular Aggregation in Monolayers from Regioselective
Substituted Cellulose Ethers with Long Alkyl Chains.
150. Characterization of Non-Crystalline Regions in Cellulose Using Deuteration and
Generalized Two-Dimensional Correlation Spectroscopy.
151. Unique Characteristics of Nematic Ordered Cellulose Derived from Its Molecular Chain Arrangements.
152. Secretion of Cellulose in Microdochium nivale.
153. Hydrophobic Sheets from Bacterial Cellulose: Surface and Mechanical Characteristics.
154. Bio-directed Epitaxial Nanodeposition of Polymers on Oriented Macromolecular
Templates.
155. Secretion of single gigantic callose fiber from a protoplast cell.
156. Surface and physical properties of biodegradable boards from bacterial cellulose.
157. Biomacromolecular tracks cntrolling direction in movements of bacteria secretion of the fibers.
158. Three dimentional architecture of biomaterials constructed by regulating the deposition of the product and the movements due to the secretion of the fibers from bacteria using biomacromolecular tracks.
159. Secretion of gigantic callose fiber from Butula protoplast.
160. Secretion mechanism of cellulose from M. Nivale.
161. Structure-mechanical property relationship in nematic ordered cellulose.
162. Developement of plates with a hydrophobic surface from bacterial cellulose.
163. Characterization of supermolecular structure of cellulose in noncrystalline domains using FTIR wirh deuteration.
164. Structural change of monolayers from cellulose derivatives under compression.
165. Enzymatic degaradation behavior of cellulase in the presence of lignin.
166. Biomacromolecular tracks cntrolling movements of bacteria.
167. Microscopic FTIR analyses as a best tool to characterize cellulosic materials.
168. Nanobiotechnology using bacteria regulated by nanotracks.
169. A concept for development of biomaterials based on cellulose.
170. A novel and fuzzy cellulose material having an ordered structure-Flexible gray rather than black and white colors.
171. Change in FTIR spectra for cellulose acetate with different distrubution of substituent.
172. Analysis on the supermolecular structure of nematic ordered cellulose using FTIR with deuteration.
173. Dependence of the substrates in the interaction of cellulase originated from Trichoderma viride.
174. AFM observation of monolayers from cellulose derivatives.
175. Change in FTIR spectra of Tunicate and Cladophora cellulose microfibrils attributed to enzymatic degardation process .
176. Physical properties of Nematic ordered cellulose.
177. Biomacromolecular tracks to simultaneously control both secretion and formation of bacterial cellulose from Acetobacter xylinum.
178. Bacterial recognition of surface of cellulose: Biomacromolecular rail.
179. Biodegaradation process of microcrystalline cellulose by brown rot fangi (21) -Mocrocrystals of cellulose in the degrated wood.
180. Minor change in Tunicate cellulose microfibrils during the enzymatic hydrolysis.
181. Monolayer structure of O-alkylcellulose ethers having controlled distribution of substituents.
182. Facile preparation of cellulose polymorphs from nematic ordered cellulose.
183. Characterization of the supermolecular structure of Nematic Ordered Cellulose using deuteration and FTIR.
184. Monolayer of O-alkylcellulose ethers having controlled distribution of substituents.
185. Characterization of noncrystalline domains in cellulose using FTIR deuteration monitoring analysis.
186. New Concepts and Uses for a Well- known Industrial Biopolymer, Cellulose.
187. Analysis of intramolecular hydrogen bonds in cellulosic solution.
188. Facile crystallization of nematic ordered cellulose obtained from drawing of cellulose.
189. Novel structure of cellulose named “Nematic Ordered Cellulose”.
190. Biomacromolecular Rails:A New Device to Control Movements of Bacteria.
191. Relashionship between cellulose supermolecular structure and the hydrolyzing behavior of cellulase.
192. Supermolecular architecture of cellulose influenced by orientation/drawing.
193. Enzymatic degradation behavoir of cellulase for Cladophora cellulose microfibrils.
194. Crystallization of Nematic ordered cellulose.
195. Properties of domains in endoglucanaseⅡ originated from Trichoderma reesei.
196. Influence of coagulants on the selfaggregation of cellulose towards noncrystalline domains in the film.
197. Preparation of monolayer from regioselectively substituted cellulose ethers with long alkyl side chains.
198. Surface analysis of cellulose crystalline structure using high resolution atomic force microscopy (II).
199. Characterization of the novel supermolecular structure of cellulose, "Nematic ordered cellulose".
200. Analysis on the supermolecular structure of tunicate cellulose microfibrils using an image-analyzing techinique for electronmicrophotographs.
201. Characterization of cellulose gel formed under various solvent-exchanging conditions.
202. Surface analysis of cellulose crystalline structure using high resolution atomic force microscopy.
203. Biodegaradation process of microcrystalline cellulose by brown rot fangi (20) -Change in degree of polymerization of cellulose-.
204. Average Distance between Two Molecules in The Noncrystalline Domains of Cellulose.
205. Facile Crystallization of Nematic-ordered Cellulose Obtained from Drawing of Water-Swollen Cellulose.
206. Characterization of A Novel Structure of Cellulose Named "Nematic ordered Cellulose".
207. Hydrogen Bonds in Regioselectively Substituted 3-O-Methylcellulose.
208. Supermolecular Archtecture of Cellulose in Some Native and Artificial Systems.
209. Structure, function and formation mechanism of wood cell walls.
210. Hydrogen Bonds in Cellulose ⅡCrystals.
211. Analysis on the orientation of mercerized Quasi-tactic cellulose films.
212. Supermolecular Architecture of Wood Cell Wall Cellulose:Characterization Using Aqueous-mode Atomic Force Microscopy (II).
213. Crystallization of Quasi-tactic cellulose.
214. Hydrogen bonds engaged in celluloseII crystalline domains.
215. A novel supermolecular structure of cellulose:“Quasi-tactic”.
216. Fluorecent spectroscopic analyses on the interaction between cellulase and the substrate.
217. CrystallinePhase Transition from Cellulose Ia to Ib by Heat Treatment.
218. Supermolecular Architecture of Wood Cell Wall Cellulose:Characterization Using Infrared Microspectroscopy and Aqueous-mode Atomic Force Microscopy.
219. Crystallization of Drawn Cellulose Films Prepared from Water-Swollen Cellulose.
220. Characterization of Amorphous Domains in Cellulose Using a FTIR Deuteration Monitoring Analysis.
221. Enzymatically Produced I-Beta Crystalline Domains of Cellulose.
222. Enzymatic Degradability of Regioselectively Substituted Water-soluble Cellulose Derivatives.
223. Biodegaradation process of microcrystalline cellulose by brown rot fangi (19).
224. Electron diffraction Image analyses on enzymatically produced short microrystalline cellulose.
225. Influence of Substituents in Cellulose Derivatives on Enzymatic Hydrolysis.
226. Analysis of Orientation of Beta-Glucan Chains in Quasi-tactic Cellulose” Using Deuteration and Polarized FTIR.
227. Crystallization of Cellulose starting from “Quasi-tactic Cellulose ”.
228. Molecular Images of Single Beta-Glucan Chains from a New Form of Cellulose Termed “Quasi-tactic”.
229. Enzymatically Produced I-beta Crystalline Domains of Cellulose.
230. Supermolecular Architecture of Wood Cell Wall Crystalline Cellulose.
231. Kinetic Study of Cellulase for Water-soluble Cellulose Derivatives.
232. Molecular Images of Single Glucan Chains in Ordered Non-crystalline Regions of Cellulose.
233. AFM Liquid Mode Analysis of Freshly-Formed Cellulose Microfibrils of the Developing Coniferous Tracheid Cell Walls.
234. AFM Characterization in Fluid for Nascent Wood Cell Wall Cellulose Microfiburils.
235. Characterization of supermolecular structure of cellulose microfibrils using image analyses of TEM images.
236. Characterization of intramolecular hydrogen bonds engaged in the aqueous solution of methylcellulose.
237. Interaction between cellulase and the substrate using fluorescent spectroscopy.
238. Crystallization of stretched films from water-swollen celulose gels.
239. Characterization of ordered cellulose films using FTIR after deuteration.
240. Characterization of ordered, noncrystalline supermolecular structure of cellulose.
241. Characterization of mercerized Quasi-tactic cellulose films using FTIR after deuteration
 .
242. Molecular image of cellulose in "Quasi-tactic" cellulose as a novel supermolecular structure.
243. Supermolecular architecture of cellulose in cell wall formation (1).
244. Characterization of supermolecular structure of cellulose microfibrils using image analyses of microdiffraction images.
245. Formation of hydrogen bonds in a methylcellulose/DMSO/water system.
246. Regulation of supermolecular structure of cellulose by crystallization of Quasi-tactic cellulose.
247. Characteriztion of hydrogen bondes in the aqueous solution of regioselectively methylated cellulose derivatives.
248. Gelation mechanism of regioselectively methylated cellulose derivatives.