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Takane Kaneko Last modified date:2019.06.05

Assistant Professor / Agricultural Bioresource Sciences
Department of Bioresource Sciences
Faculty of Agriculture


Graduate School
Undergraduate School
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Homepage
http://www.agr.kyushu-u.ac.jp/lab/doubutsu/
The introduction of the zoology laboratory .
Academic Degree
Agriculture
Country of degree conferring institution (Overseas)
No
Field of Specialization
Developmental Biology
Total Priod of education and research career in the foreign country
00years00months
Outline Activities
Research activity: Research theme "The research on fertilization mechanism and differentiation mechanism of the reproductive cell in the small mammal". Using the small mammal, especially Suncus of Insectivora which are a basis of Eutheria, I aim at clarifying the interaction of spermatozoa and cumulus-oocyte complexes by the biochemical, morphological and molecularly-biological technique. In the differentiation of reproductive cell, to understand the mechanisms of testicular germ cell differentiation, it is necessary to isolate genes which are specifically expressed in a certain stage of testis development. I apply a differential display method to identify genes which are developmentally upregulated during rat testis development.
Educational activity: In experiment, practical training, practice, and research guidance for the paper preparation, the education is carried out on the basis of zoology, embryology, cell biological study.
Society activity: For senior high school student and general people, the result of the zoology research is opened to public.
Research
Research Interests
  • Ovulation and Fertilization in the house musk shrew, Suncus Murinus.
    keyword : Suncus Murinus, acrosome reaction, fertilization, sperm, cumulus-oocyte complex
    2000.04In the oviduct of the musk shrew, Suncus murinus, spermatozoa with an intact acrosome bind to the surface of the cumulus oophorus, and display a vesiculation of the sperm plasma membrane and outer acrosomal membrane. These reacting spermatozoa appear to kill the adjacent cumulus cells on one side and penetrate through these cells. All spermatozoa penetrating further through the cumulus and entering the peri-zonal space had shed the giant acrosome. The cumulus in Suncus may therefore function as an essential mediator of fertilization by inducing the acrosome reaction, as it appears to in another shrew - Cryptotis parva. Reacted spermatozoa seem likely to attach or bind to the zona pellucida by barbs exposed on the elaborate apical body (perforatorium), and penetrate it mechanically by the one dimensional lateral beat of the tail. At present, it is observed that the micro structure in the interaction of spermatozoa and cumulus-oocyte complexes of the Suncus. In addition, by biochemistry and molecularly-biological technique, the acrosome is analyzed..
  • Differentiation mechanism of germ cells in small mammalian.
    keyword : development biology, sperm, germ cell, differentiation, spermatogenesis
    2002.10Mammalian spermatogenesis is an excellent model system to study regulation of gene expression during differentiation. Postnatal development of the rat seminiferous tubule can be subdivided into three parts: (1) a premeiotic phase characterized by mitotic division of diploid spermatogonia; (2) a meiotic phase, which leads to the formation of haploid round spermatids; and (3) a post-meiotic phase, which includes the remarkable morphological changes required for spermatozoa formation (spermiogenesis). To understand the mechanisms of testicular germ cell differentiation, it is necessary to isolate genes which are specifically expressed in a certain stage of testis development. I apply a differential display method to identify genes which are developmentally upregulated during rat testis development..
Academic Activities
Papers
1. Kaneko, T., Minohara, T., Shima, S., Yoshida, K., Fukuda, A., Iwamori, N., Inai, T. and Iida, H.,, A membrane protein, TMCO5A, has a close relationship with manchette microtubules in rat spermatids during spermiogenesis, Molecular Reproduction and Development, 10.1002/mrd.23108, 86, 3, 330-341, 2019.03, We isolated the transmembrane and coiled-coil domains 5A (Tmco5A) gene using polymerase chain reaction-based subtraction technique and showed that Tmco5A was predominantly expressed in rat testes starting at 4 weeks of postnatal development. When expressed in COS7 cells, TMCO5A was found to be distributed in the endoplasmic reticulum-nuclear membrane (ER-NM) of cells as a membrane-associated protein, while TMCO5AΔC lacking the transmembrane region (TM) mislocalized and diffused throughout the cytoplasm. The result suggested that TM is responsible for the retention of TMCO5A at the ER-NM. Immunocytochemical and immunoblotting analyses indicated that TMCO5A was localized along the posterior part of the nuclei in both round and elongated rat spermatids but disappeared from epididymal spermatozoa. Double immunolabeling of isolated spermatids with the anti-TMCO5A and the anti-β tubulin antibodies showed that TMCO5A was always found to be closely associated with developing manchette microtubules but did not completely colocalize with them. On the other hand, we found that almost all TMCO5A colocalized with SUN4, a linker of nucleoskeleton and cytoskeleton complex protein present at the posterior part of spermatid nuclei. These data suggested that TMCO5A is located closer to the nuclei than the manchette microtubules. It is likely that TMCO5A, in association with manchette microtubules, is involved in the process of spermiogenesis..
2. Yusuke Sato, Hideaki Ohtsubo, Naohiro Nihei, Takane Kaneko, Yoriko Sato, Shin Ichi Adachi, Shinji Kondo, Mako Nakamura, Wataru Mizunoya, Hiroshi Iida, Ryuichi Tatsumi, Cristina Rada, Fumiaki Yoshizawa, Apobec2 deficiency causes mitochondrial defects and mitophagy in skeletal muscle, FASEB Journal, 10.1096/fj.201700493R, 32, 3, 1428-1439, 2018.03, Apobec2 is a member of the activation-induced deaminase/apolipoprotein B mRNA editing enzyme catalytic polypeptide cytidine deaminase family expressed in differentiated skeletal and cardiac muscle. We previously reported that Apobec2 deficiency in mice leads to a shift in muscle fiber type, myopathy, and diminished muscle mass. However, the mechanisms ofmyopathy caused by Apobec2 deficiency and its physiologic functions are unclear. Here we show that, although Apobec2 localizes to the sarcomeric Z-lines in mouse tissue and cultured myotubes, the sarcomeric structure is not affected in Apobec2-deficient muscle. In contrast, electron microscopy reveals enlarged mitochondria and mitochondria engulfed by autophagic vacuoles, suggesting that Apobec2 deficiency causes mitochondrial defects leading to increased mitophagy in skeletal muscle. Indeed, Apobec2 deficiency results in increased reactive oxygen species generation and depolarized mitochondria, leading to mitophagy as a defensive response. Furthermore, the exercise capacity of Apobec2-/- mice is impaired, implying Apobec2 deficiency results in ongoing muscle dysfunction. The presence of rimmed vacuoles in myofibers from 10-mo-old mice suggests that the chronic muscle damage impairs normal autophagy. We conclude that Apobec2 deficiency causes mitochondrial defects that increase muscle mitophagy, leading to myopathy and atrophy. Our findings demonstrate that Apobec2 is required for mitochondrial homeostasis to maintain normal skeletal muscle function..
3. Kaneko, T., Toshimori, K. and Iida H., Subcellular localization of MS4A13 isoform 2 in mouse spermatozoa., Reproduction, doi: 10.1530/REP-17-0477, 154, 6, 843-857, 2017.12, To identify upregulated genes during the development of spermatozoa, we performed PCR-selected subtraction analysis of testes RNA
samples from 10-day-old and 12-week-old shrews. A transcript, highly homologous to two mouse transcripts, Ms4a13-1 and Ms4a13-
2, was differentially regulated. Ms4a13-2, but not Ms4a13-1, was shown to be primarily expressed in mouse testes in an agedependent
manner. Ms4a13-2 cDNA contains an open-reading frame of 522 nucleotides, encoding a protein of 174 amino acids, with
predicted molecular mass, 19,345 Da. MS4A13-2 protein was expressed along the periphery of nuclei of round and elongated
spermatids (steps 3–16) in adult mouse testes, and in the equatorial region of the heads of fresh mature mouse spermatozoa. In
addition, MS4A13-2 was found to localize to the outer acrosomal membrane in the equatorial region of heads in fresh spermatozoa.
In acrosome-reacted spermatozoa, the MS4A13-2 expression extended to the entire sperm head including the postacrosomal region
and acrosomal cap. MS4A family proteins are known to facilitate intracellular protein–protein interactions as ion channel/adaptor
proteins by oligomerization, and have important regulatory roles in cellular growth, survival and activation. We report that the MS4A
family member, MS4A13-2, may form oligomers in sperm membranes, which may be involved in an interaction with the zona
pellucida or cumulus during fertilization..
4. Shotaro Nishimura, Miyu Yamashita, Takane Kaneko, Fuminori Kawabata, Shoji Tabata, Cytokeratin-positive folliculo-stellate cells in chicken adenohypophysis, Animal Science Journal, 10.1111/asj.12866, 88, 11, 1835-1841, 2017.11, Folliculo-stellate (FS) cells are non-endocrine cells found in the adenohypophysis and are identified in many animals by the S100 protein marker. Although keratin is another FS marker in several animals, there is no information on localization of keratin in the avian adenohypophysis. In this study, localization of cytokeratin in chicken adenohypophyseal cells was investigated immunohistochemically. Basic cytokeratin (bCK)-positive cells were arranged radially in the cell cords with their cytoplasmic processes reaching the basal lamina. The cell bodies encircled a follicle in the center of the cell cord. Furthermore, the bCK-positive cells were also S100B-positive. Growth hormone, prolactin, adrenocorticotrophic hormone, and luteinizing hormone β-subunit did not co-localize with the bCK-positive cells. In addition, the bCK-positive cells had a laminin-positive area in their cytoplasm. Transmission electron microscopy observed agranular cells equipped with several microvilli that encircled a follicle. These results indicate that bCK-positive cells in the chicken adenohypophysis may be a predominant FS cell population and produce laminin. It is suggested that they function as sustentacular cells to sustain the adjacent endocrine cells and the structure of the cell cords in the chicken adenohypophysis..
5. Airi Yamaguchi, Takane Kaneko, hiroshi iida, Molecular Cloning and Subcellular Localization of Tektin2-Binding Protein1 (Ccdc 172) in Rat Spermatozoa., Journal of Histochemistry and Cytochemistry, 62, 4, 286-297, 2014.04.
6. Takiguchi, H., Murayama, E., Kaneko, T., Kurio, H., Toshimori, K. and Iida, H. , Characterization and subcellular localization of Tektin 3 in rat spermatozoa., Molecular Reproduction and Development, 78, 611-620, 2011.08.
7. Kurio, H., Hatsuda, H., Murayama, E., Kaneko, T. and Iida, H., Identification of CEACAM6 as an intermediate filament-associated protein expressed in Sertoli cells of rat testis., Biology of Reproduction, 85, 924-933, 2011.06.
8. Kaneko, T., Iwamoto, S., Murayama, E., Kurio, H., Inai, T., Oda, S. and Iida, H. , Immunolocalization of Spetex-1 at the connecting piece in spermatozoa of the musk shrew (Suncus murinus)., Zoological Science, 28, 6, 444-452, 2011.06.
9. Shimasaki, S., Yamamoto, E., Murayama, E., Kurio, H., Kaneko, T., Shibata, Y., Inai, T. and Iida, H. , Subcellular Localization of Tektin2 in Rat Sperm Flagellum., Zoological Science, 27, 755-761, 2010.09.
10. Kaneko, T., Murayama, E., Kurio, H., Yamaguchi, A. and Iida, H. , Characterization of Spetex-1, a new component of satellite fibrils associated with outer dense fibers in the middle piece of rodent sperm flagella. , Molecular Reproduction and Development, 77, 363-372, 2010.04.
11. Yano, R., Matsuyama, T., Kaneko, T., Kurio, H., Murayama, E., Toshimori, K. and Iida, H. , Bactericidal/permeability - increasing protein (BPI) is associated with the acrosome region of rodent epididymal spermatozoa. , Journal of Andrology, 31, 201-214, 2010.03.
12. Morphological Studies of Functional Molecules Building Mammalian Sperm Flagella..
13. The reexamined mammalian systematics..
14. Kurio, H., Murayama, E., Kaneko, T., Shibata, Y., Inai, T. and Iida, H., Intron 
retention generates a novel isoform of CEACAM6 that possibly acts as 
an adhesion molecule at ectoplasmic specialization between spermatids 
and sertoli cells in rat testis., Biology of Reproduction, 79:1062-1073., 2008.08.
15. Murayama, E., Yamamoto, E., Kaneko, T., Shibata, Y., Inai, T. and Iida, H., Tektin5, a new TEKTIN family member, is a component of the middle piece of flagella in rat spermatozoa., Molecular Reproduction and Development, 75:650–658, 2008.04.
16. Murayama, E., Katoh, M., Kanebayashi, A., Kaneko, T., Shibata, Y., Inai, T. and Iida, H. , Germ cell-less like 2 protein is a new component of outer dense fibers in rat sperm flagella. , Reproduction, 134:749-756, 2007.12.
17. Acrosome reaction of suncus spermatozoa. (2007) Kaneko, T. and Iida, H. Laboratory Animal Technology and Science, 19(4): 25-29..
18. Kaneko, T., Mori, T. and Ishijima, S., Digital image analysis of the flagellar beat of activated and hyperactivated Suncus spermatozoa., Molecular Reproduction and Development, 74:478-485, 2007.01.
19. Doiguchi, M., Kaneko, T., Urasoko, A., Nishitani, H. and Iida, H., Identification of a heat shock protein Hsp40, DjB1, as an acrosome- and a tail-associated component in rodent spermatozoa., Molecular Reproduction and Development, 74:223-232, 2007.01.
20. Iida, H., Noda, M., Kaneko, T., Doiguchi, M. and Mori, T., Identification of rab12 as a vesicle-associated small GTPase highly expressed in Sertoli cells of rat testis., Molecular Reproduction and Development, 10.1002/mrd.20294, 71, 2, 178-185, 71:178-185, 2005.05.
21. Iwamoto, Y., Kaneko, T., Ichinose, J., Mori, T., Shibata, Y., Toshimori, K. and Iida, H., Molecular cloning of rat Spetex-2 family genes mapped on chromosome 15p16, encoding a 23-kilodalton protein associated with the plasma membranes of haploid spermatids., Biology of Reproduction, 10.1095/biolreprod.104.032516, 72, 2, 284-292, 72:284-292, 2005.02.
22. Iida, H., Yamashita, H., Doiguchi, M. and Kaneko, T., Molecular cloning of rat spergen-3, a spermatogenic cell-specific gene-3, encoding a novel 75 kDa protein bearing EF-hand motifs., Journal of Andrology, 25, 6, 885-892, 25:885-892, 2004.11.
23. Iida, H., Ichinose, J., Kaneko, T., Mori, T. and Shibata Y., Complementary DNA cloning of rat Spetex-1, a spermatid-expressing gene-1, encoding a 63 kDa cytoplasmic protein of elongate spermatids., Molecular Reproduction and Development, 10.1002/mrd.20101, 68, 4, 385-393, 68:385-393, 2004.08.
24. Kaneko, T., Iida, H., Bedford, J. M., Oda, S. and Mori, T., Mating-induced cumulus-oocyte maturation in the shrew, Suncus murinus., Reproduction, 10.1530/rep.0.1260817, 126, 6, 817-826, 126:817-826, 2003.12.
25. Iida, H., Mori, T., Kaneko, T., Urasoko, A., Yamada, F. and Shibata, Y., Disturbed spermatogenesis in mice prenatally exposed to an endocrine disruptor, Bisphenol A., Mammal Study, 27: 73-82, 2002.05.
26. Kaneko, T., Iida, H., Bedford, J. M. and Mori, T., Spermatozoa of the shrew, Suncus murinus, undergo the acrosome reaction and then selectively kill cells in penetrating the cumulus oophorus., Biology of Reproduction, 10.1095/biolreprod65.2.544, 65, 2, 544-553, 65: 544-553, 2001.05.
27. Iida, H., Kaneko, T., Tanaka, S. and Mori, T., Association of the developing acrosome with multiple small Golgi units, the Golgi satellites, in spermatids of the musk shrew, Suncus murinus., Journal of Reproduction and Fertility, 10.1530/reprod/119.1.49, 119, 1, 49-58, 119: 49-58, 2000.01.
Works, Software and Database
1. .
2. .
3. .
4. Kyushu University Museum:Chiropteran Databases
[URL].
5. Kyushu University Museum:Insectivora and Rodentia Databases
[URL].
6. Kyushu University Museum:Chiropteran, Avian, Amphibian, Reptile Databases
[URL].
Presentations
1. Localization of CCDC176 in rat spermatozoa.
Kaneko T., Sunano H., Sasaki T, Fujita I., Yoshiura Y., Iwamori N. and Iida H.
The 72th Kyushu meeting of the Zoological Society of Japan..
2. 金子たかね, マウスの受精におけるMS4A13の機能解析, 第2回九州大学女性研究者 ダイバーシティシンポジウム, 2019.03.
3. Functional Analysis of MS4A13 in Mouse Fertilization.
Kaneko T., Toshimori K., Yoshiura Y., and Iida. H.
The 89th annual meeting of the Zoological Society of Japan..
4. Iida H., Kaneko T., Yoshida K., Moshida I. and Iwamori N.
The 74th annual meeting of the Japanese Society of Microscopy..
5. Functional Analysis of MS4A13 in Mouse Sperm Head.
Kaneko, T., Toshimori, K., Yoshiura, Y., Sasaki, T. and Iida, H.
The 74th annual meeting of the Japanese Society of Microscopy..
6. Iida H., Kaneko T., Yoshida K., Moshida I. and Iwamori N.
The 71th Kyushu meeting of the Zoological Society of Japan..
7. Kaneko, T., Toshimori, K. and Iida. H.
Chugoku, Shikoku and Kyushu meeting of the Zoological Society of Japan..
8. Kaneko, T. and Iida. H.
The 70th Kyushu meeting of the Zoological Society of Japan..
9. Study of reproduction of Suncus murinus.
Kaneko, T.
The 11th Research Society of Suncus murinus..
10. 山下 未侑   , 西村 正太郎, 金子 たかね, 川端二功, 田畑 正志, Folliculo-stellate cells produce a basal lamina component in the chicken adenohypophysis., 17th AAAP ANIMAL SCIENCE CONGRESS, 2016.08.
11. Localization of Ms4a13 in mouse spermatozoa.
Kaneko, T. and Iida. H.
The 85th annual meeting of the Zoological Society of Japan..
12. Identification of Tmco5A in Mammalian germ cell.
Fukuda, A., Kaneko, T. and Iida. H.
The 85th annual meeting of the Zoological Society of Japan..
13. Hukuda, A., Kaneko, T. and Iida. H.
The 67th Kyushu meeting of the Zoological Society of Japan..
14. Kaneko, T. and Iida. H.
The 67th Kyushu meeting of the Zoological Society of Japan..
15. Immunolocalization of Tektin2 and Tektin3 in Suncus spermatozo.
Yoshimatu, S., Kaneko, T. and Iida. H.
The 84th annual meeting of the Zoological Society of Japan..
16. Araki, A., Kaneko, T. and Iida. H.
The 66th Kyushu meeting of the Zoological Society of Japan..
17. APOBEC2 Deficiency Causes Increased Autophagy and Abnormal Mitochondria in Skeletal Muscle.
Sato, Y., Ohtsubo, H., Kaneko, T., Mizunoya, W., Nakamura, M., Tatsumi, R., Iida, H., Ikeuchi, Y., Yoshizawa, F., Sugahara, K., Neuberger, M. S. and Rada, C.
2012 FASEB Summer Research Conference on “Skeletal Muscle Satellite & Stem Cells”.
18. Subcellular Localization of Tektin1 in Mammalian Spermatozoa.
Oiki S., Kaneko, T. and Iida. H.
The 83th annual meeting of the Zoological Society of Japan..
19. Ectopic Expression of CEACAM6 and CEACAM6-L in Rat Seminiferous Epithelium .
Kurio, H., Murayama, E., Kaneko, T. and Iida. H.
The 8-th International AFSF Joint Symposium between Japan and Korea..
20. Subcellular Localization of Tektin2 binding protein (Tekitn2-BP1) in Rat Sperm Flagellum.
Yamaguchi Airi, Murayama, E., Kaneko, T. and Iida. H.
The 82th annual meeting of the Zoological Society of Japan..
21. Ectopic Expression of CEACAM6 and CEACAM6-L in Rat Seminiferous Epithelium .
Kurio, H., Murayama, E., Kaneko, T. and Iida. H.
The 82th annual meeting of the Zoological Society of Japan..
22. Tektin5, a new TEKTIN family member, interacts with Tektin4 and Odf1.
Murayama, E., Kurio, H., Kaneko, T. and Iida. H.
The 82th annual meeting of the Zoological Society of Japan..
23. Expression of Ceacam6 in rat seminiferous epithelium.
Kurio, H., Murayama, E., Kaneko, T. and Iida. H.
The 64th Kyushu meeting of the Zoological Society of Japan..
24. Localization of Tektin2 BP1 in the flagella of rat spermatozoa.
Yamaguchi, A., Murayama, E., Kaneko, T. and Iida. H.
The 64th Kyushu meeting of the Zoological Society of Japan..
25. Localization of Tektin5 BP1 in the flagella of rat spermatozoa.
Murayama, E., Kurio, H., Kaneko, T. and Iida. H.
The 64th Kyushu meeting of the Zoological Society of Japan..
26. Ectopical expression of Ceacam6 and Ceacam6-L in rat seminiferous epithelium.
Kurio, H., Murayama, E., Kaneko, T. and Iida. H.
The 64th annual meeting of the Japanese Society of Microscopy..
27. Immunolocalization of Splunc3 protein in the rat sperm flagella.
Saeki, R., Kaneko, T. and Iida. H.
The 52th Kyushu meeting of the Japanese Society of Microscopy..
28. Immuno localization of HRAS-like suppressor family member 5 (Hrasls 5) in rat spermatozoa.
Ryu, K., Kaneko, T. and Iida. H.
The 52th Kyushu meeting of the Japanese Society of Microscopy..
29. Localization of Tektin2 in mammalian sperm flagella.
Shimasaki, S., Murayama, E., Kaneko, T. and Iida. H.
The 52th Kyushu meeting of the Japanese Society of Microscopy..
30. Localization of Tektin3 in rat sperm flagella.
Takiguchi, H., Murayama, E., Kaneko, T. and Iida. H.
The 52th Kyushu meeting of the Japanese Society of Microscopy..
31. Immunolocalization of Splunc3 protein in the rat sperm flagella.
Saeki, R., Kaneko, T. and Iida. H.
The 81th annual meeting of the Zoological Society of Japan..
32. Immuno localization of HRAS-like suppressor family member 5 (Hrasls 5) in rat spermatozoa.
Ryu, K., Kaneko, T. and Iida. H.
The 81th annual meeting of the Zoological Society of Japan..
33. Identification of Spetex-1 in mammalian sperm flagella.
Kaneko, T., Murayama, E., Kurio, H. and Iida. H.
The 81th annual meeting of the Zoological Society of Japan..
34. Localization of Tektin3 in rat sperm flagella.
Takiguchi, H., Murayama, E., Kaneko, T. and Iida. H.
The 81th annual meeting of the Zoological Society of Japan..
35. Bactericidal/permeability- increasing protein (BPI) is associated with the acrosome region of rodent epididymal spermatozoa.
H. Iida, T. Kaneko, H. Kurio, E. Murayama.
11th International Symposium on Spermatology, Okinawa, June 24-29, 2010..
36. Ectopic expression of Ceacam6 and Ceacam6-L in rat seminiferous epithelium.
H. Kurio, E. Murayama, T. Kaneko, H. Iida.
11th International Symposium on Spermatology, Okinawa, June 24-29, 2010..
37. Molecular localization of Spetex-1 in mammalian spermatozoa.
T. Kaneko, E. Murayama, H. Kurio, H. Iida.
11th International Symposium on Spermatology, Okinawa, June 24-29, 2010..
38. Identification of HRAS-like suppressor family, member 5 (Hrasls) in rat spermatozoa.
Ryu, K., Kaneko, T. and Iida. H.
The 63th Kyushu meeting of the Zoological Society of Japan..
39. Identification of Tektin3 in rat sperm flagellum.
Takiguchi, H., Murayama, E., Kaneko, T. and Iida. H.
The 63th Kyushu meeting of the Zoological Society of Japan..
40. Identification of Spetex-1 in sperm flagellum in Mammalia.
Kaneko, T., Murayama, E., Kurio, H. and Iida. H.
The 63th Kyushu meeting of the Zoological Society of Japan..
41. Localization of Gcl-2 BP1 in the middle piece of flagella of rat spermatozoa.
Murayama, E., Kurio, H., Kaneko, T. and Iida. H.
The 51th Kyushu meeting of the Japanese Society of Microscopy..
42. Identification of Spetex-1 in sperm flagellum in Mammalia.
Kaneko, T., Murayama, E., Kurio, H. and Iida. H.
The 51th Kyushu meeting of the Japanese Society of Microscopy..
43. Immunolocalization of Spetex-1 at the segmented column in Suncus sperm flagellum.
Iwamoto, S., Kaneko, T. and Iida, H.
The 80th annual meeting of the Zoological Society of Japan..
44. Subcellular localization of Tektin2 and Tektin2-binding protein in rat sperm flagella.
Shimasaki, S., Murayama, E., Kaneko, T. and Iida, H.
The 80th annual meeting of the Zoological Society of Japan..
45. Localization of Gcl-2 BP1 in the middle piece of flagella of rat spermatozoa.
Murayama, E., Kurio, H., Kaneko, T. and Iida, H.
The 80th annual meeting of the Zoological Society of Japan..
46. Molecular network of skeletal proteins in rodent sperm flagella
Kaneko, T., Murayama, E., Kurio, H. and Iida, H.
The 80th annual meeting of the Zoological Society of Japan..
47. Subcellular localization of Tektin2 and Tektin2-binding protein in rat sperm flagella.
Shimasaki, S., Murayama, E., Kurio, H., Kaneko, T. and Hiroshi Iida
The 42th annual meeting of the Japanese Soccciety of Developmental Biologists of Japan..
48. Identification and localization of Tektin2-binding protein in rat sperm flagella.
Shimasaki, S., Murayama, E., Kaneko, T. and Iida. H.
The 62th Kyushu meeting of the Zoological Society of Japan..
49. Localization of a binding protein Gcl-2 at the rat sperm flagella.
Murayama, E., Kurio, H., Kaneko, T. and Iida. H.
The 62th Kyushu meeting of the Zoological Society of Japan..
50. Identification of Spetex-1 in sperm flagellum in the musk shrew, Suncus murinus.
Iwamoto, S., Kaneko, T. and Iida. H.
The 62th Kyushu meeting of the Zoological Society of Japan..
51. Localization of a haploid-specific novel protein Nu3 at the rat sperm flagella.
Hayashida, S., Murayama, E., Kurio, H., Kaneko, T. and Iida. H.
The 79th annual meeting of the Zoological Society of Japan..
52. Immunolocalization of Tarm protein on the mitochondrial surface of rat sperm flagella.
Kawarada, K., Murayama, E., Kaneko, T. and Iida, H.
The 79th annual meeting of the Zoological Society of Japan..
53. Expression and localization of Ceacam6-L that acts as a novel adhesion molecule between Sertoli cells and spermatids in rat testis.
Kurio, H., Murayama, E., Kaneko, T. and Iida, H.
The 79th annual meeting of the Zoological Society of Japan..
54. Identification of Ex2 expressed in sperm acrosome in rat and suncus.
Oda, K., Murayama, E., Kurio, H., Kaneko, T. and Iida. H.
The 79th annual meeting of the Zoological Society of Japan..
55. Intron retention generates a novel isoform of Ceacam6 that acts as an adhesion molecule at ectoplasmic specialization between Sertoli cells and spermatids in rat testis.
Kurio, H., Murayama, E., Kaneko, T. and Iida, H.
The 41th annual meeting of the Japanese Soccciety of Developmental Biologists of Japan..
56. Identification of Spetex-1 and Tektin 4 as outer dense fiber-associated molecules in flagella of rodent spermatozoa.
Kaneko, T., Murayama, E., Kurio, H. and Iida, H.
The 64th annual meeting of the Japanese Society of Microscopy..
57. Identification of Ex2 expressed in sperm acrosome.
Oda, K., Kaneko, T. and Iida. H.
The 61th Kyushu meeting of the Zoological Society of Japan..
58. Localization of Nu3 expressed in the haploid germ cells.
Hayashida, S., Kaneko, T. and Iida. H.
The 61th Kyushu meeting of the Zoological Society of Japan..
59. Intron retention generates a novel isoform of Ceacam6 that acts as an adhesion molecule at ectoplasmic specialization between Sertoli cells and spermatids in rat testis.
Kurio, H., Murayama, E., Kaneko, T. and Iida. H.
The 61th Kyushu meeting of the Zoological Society of Japan..
60. Molecule localization of Tektin2 and Tektin2 - binding protein in sperm flagellum.
Murayama, E., Yamamoto, E., Kurio, H., Kaneko, T. and Iida. H.
The 61th Kyushu meeting of the Zoological Society of Japan..
61. Fertilization in the house musk shrew, Suncus murinus.
Kaneko, T.
The 113th annual meeting of the Japanese Association of Anatomists.
The Third annual meeting of society for the study of Suncus..
62. Localization of BPI secreted from epididymal epithelium.
Yano, R., Iida, H. and Kaneko, T.
The 78th annual meeting of the Zoological Society of Japan..
63. Cloning of a novel immunoglobulin superfamily gene expressed in rat testis.
Kurio, H., Kaneko, T. and Iida, H.
The 78th annual meeting of the Zoological Society of Japan..
64. Localization of rat gcl-2 at the cortex of outer dense fiber in rat sperm flagella.
Iida, H., Murayama, E. and Kaneko, T.
The 78th annual meeting of the Zoological Society of Japan..
65. Localization of Tektin5, a new TEKTIN family member, in the middle piece of flagella of rat spermatozoa.
Murayama, E., Yamamoto, E., Kaneko, T. and Iida, H.
The 78th annual meeting of the Zoological Society of Japan..
66. Tektin2 specifically expressed in the haploid germ cells is localized in rat sperm flagella.
Yamamoto, E., Murayama, E., Kaneko, T. and Iida, H.
The 78th annual meeting of the Zoological Society of Japan..
67. Identification of Spetex-1 and Tektin 4 as outer dense fiber-associated molecules in flagella of rodent spermatozoa.
Kaneko, T., Kwarada, K., Sada, J. and Iida, H.
The 78th annual meeting of the Zoological Society of Japan..
68. Characterization of BPI associated with the acrosomal region of epididymal spermatozoa..
Iida. H., Yano, R., Kaneko, T. and Kurio, H.
The 40th annual meeting of the Japanese Soccciety of Developmental Biologists of Japan..
69. Spetex-1, a component of satellite fibrils in rodent sperm flagella, interacts with Tektin 4.
Kaneko, T., Kawarada, K., Sada, J. and Iida. H.
The 40th annual meeting of the Japanese Soccciety of Developmental Biologists of Japan..
70. Identification of Spetex-1 as a component of satellite fibrils associated with outer dense fiber of flagella in rodent spermatozoa.
Kaneko, T., Kawarada, K., Kurio H., Sada, J. and Iida. H.
The 60th Kyushu meeting of the Zoological Society of Japan..
71. Molecular localization of Spetex-1 in the middle piece of flagellum in rodent spermatozoa.
Iida, H., Kaneko, T., Murayama, E. and Yamamoto, E.
The 62th Kyushu meeting of The Japanese Association of Anatomists..
72. comparison of neck and upper middle piece of mammalian spermatozoa.
Kaneko, T., Ishijima, S. and Mori, T.
The 77th annual meeting of the Zoological Society of Japan..
73. Identification of heat shock protein Hsp40/DjB1 as an acrosome- and a tail-associated component in rodent spermatozoa.
Iida, H., Doiguchi M., Kaneko, T., Kwarada, K., Kurio, H., Sada, J. and Yano, E.
The 77th annual meeting of the Zoological Society of Japan..
74. Identification of Spetex-1 and Tektin 4 as outer dense fiber-associated molecules in flagella of rodent spermatozoa.
Iida, H., Kaneko, T.
20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress..
75. Molecular localization of Spetex-1 in the middle piece of flagellum in rodent spermatozoa.
Kaneko, T. and Iida, H.
The 39th annual meeting of the Development Growth and Differentiation of Japan..
76. Tektin4 is located on outer dense fibers, not associated with axonemal tubulins of flagella in rodent spermatozoa.
Iida, H. and Kaneko, T.
The 39th annual meeting of the Development Growth and Differentiation of Japan..
77. Novelties of spermatogenesis in insectivorous mammals (Lipotyphla).
Kaneko, T., Mori, T., Morita, T.and Oda, S.
The 76th annual meeting of the Zoological Society of Japan..
78. Identification of rab12 as a vesicle-associated small GTPase highly expressed in Sertoli cells of rat testis.
Noda, M., Kaneko, T., Mori, T. and Iida, H.
The 76th annual meeting of the Zoological Society of Japan..
79. DAILY TORPOR IN HOUSE MUSK SHREW, Suncus murinus.
Toyofuku, H., Takahashi, T., Morita, T., Tsuchiya, K., Kaneko, T., Mori, T. and Oda, S.
The 9th International Mammalogical Congress..
80. Molecular cloning of rat Spetex-2 family genes mapped on chromosome 15p16, encoding a 23-kilodalton protein associated with the plasma membranes of haploid spermatids.
Iwamoto, Y., Kaneko, T., Mori, T. and Iida, H.
The 75th annual meeting of the Zoological Society of Japan..
81. Identification of rab12 as a vesicle-associated small GTPase highly expressed in Sertoli cells of rat testis.
Noda, M., Kaneko, T., Mori, T. and Iida, H.
The 75th annual meeting of the Zoological Society of Japan..
82. The micro structure and functional morphology of spermatozoa, and The systematic taxonomy examination in the Apodemus speciosus.
Iida, H., Kaneko, T.
The 37th annual meeting of the Development Growth and Differentiation of Japan..
Membership in Academic Society
  • The Japanese Society of Microscopy
  • The Zoological Society of Japan
Educational
Educational Activities
Graduate school doctor later stage and master degree program :I guide the research for making doctor and master's degree paper.
Department : I guide the research for making the graduation thesis.
Other: I am in charge of zoologic sample practical training for applying for the qualification of the curator (the Kyushu Univ. museum ).
Other Educational Activities
  • 2006.06, Mr. Une lecture meeting host.
Social
Professional and Outreach Activities
Observation such as the museum for the general people by the Zoological Society of Japan
Poster session of the senior high school student by the Society of Japan
The open campus for the senior high school student
The protection of the Tsushima wildcat (The zoo in Fukuoka City).