Kyushu University Academic Staff Educational and Research Activities Database
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NOZOMU OKINO Last modified date:2018.09.02

Associate Professor / Molecular Bioscience
Department of Bioscience and Biotechnology
Faculty of Agriculture

Graduate School
Undergraduate School

Academic Degree
Country of degree conferring institution (Overseas)
Field of Specialization
Marine Resource Chemistry, Biochemistry, Microbiology
Total Priod of education and research career in the foreign country
Outline Activities
My research projects are as follows:
1. Search and Utilization of New Marine Resources
(1). Molecular cloning and functional analyses of genes from marine microbes and marine invertebrates
(2). Utilization of marine bacteria for glycobiology and glycotechnology
(3). Purification and characterization of novel sphingolipid-degrading enzyme inhibitors from marine resources
(4). Isolation, characterization and cDNA cloning of novel sphingolipid-degrading enzymes and tumor-specific lectin from marine invertebrates (jellyfish, starfish etc.)
(5). Purification, characterization and gene cloning of sphingolipid-degrading enzymes from marine bacteria
2. Molecular Mechanism for Inversion of Pathogens and Expression of Their Phathogenecity
(1). Expression cloning of hemolysin related to fish disease
(2). Functional analyses of bacterial sphingolipid-degrading enzymes
3. Glycobiology and Sphingolipid Technology
(1). Isolation of sphingolipid-degrading bacteria from patients with atopic dermatitis
(2). A novel but highly conserved gene family of neutral/alkaline ceramidases
(3). Molecular evolution of endoglycoceramidase
(4). Sphingolipid-mediated signal transduction
Research Interests
  • Analysis of sphingolipid metabolizing enzymes
    keyword : Sphingolipid, enzyme, ceramide
    2003.04Analysis of sphingolipid metabolizing enzymes.
  • Marine Biotechnology
    keyword : Poly unsaturated fatty acid, Lipid binding protein
    2003.04Analysis of sphingolipid metabolizing enzymes.
Academic Activities
1. Biological Function and the Survival of Humans
Discovery of a New Ceramidase Family
Nozomu Okino
1. Makoto Ito, Nozomu Okino, Motohiro Tani, New insight into the structure, reaction mechanism, and biological functions of neutral ceramidase, Biochimica et Biophysica Acta, 1841, 682–691, 2014.05.
2. The critical reason for production of ceramidase in Pseudomonas aeruginosa
Nozomu Okino.
1. Nozomu Okino, Hiroyoshi Wakisaka, Yohei Ishibashi, Makoto Ito, Visualization of Endoplasmic Reticulum and Mitochondria in Aurantiochytrium limacinum by the Expression of EGFP with Cell Organelle-Specific Targeting/Retaining Signals, Marine Biotechnology, 10.1007/s10126-018-9795-7, 1-11, 2018.01.
2. Nozomu Okino, Makoto Ito, Molecular mechanism for sphingosine-induced Pseudomonas ceramidase expression through the transcriptional regulator SphR, Scientific Reports, 6, 38797, 2016.12.
3. Inoue T, Okino N, Kakuta Y, Hijikata A, Okano H, Goda HM, Tani M, Sueyoshi N, Kambayashi K, Matsumura H, Kai Y, Ito M., Mechanistic insights into the hydrolysis and synthesis of ceramide by neutral ceramidase., J Biol Chem, 284(14):9566-9577, 2009.04.
4. N. Okino and M. Ito, Ceramidase Enhances Phospholipase C-induced Hemolysis by Pseudomonas aeruginosa, J. Biol. Chem. , 282, 6021-6030, 2007.03.
5. H. Monjusho, N. Okino, M. Tani, M. Maeda, M. Yoshida, and M. Ito, A Neutral Ceramidase Homologue of Dictyostelium discoideum Exhibits an Acidic pH Optimum, Biochem. J., 10.1042/BJ20030652, 376, 473-479, 376, pp473-479, 2003.12.
6. N. Okino, X. He, S. Gatt, K. Sandhoff, M. Ito, and E. H. Schuchman, The Reverse Activity of Human Acid Ceramidase, J. Biol. Chem., 10.1074/jbc.M303310200, 278, 32, 29948-29953, 278, pp29948-29953, 2003.08.
7. X. He, N. Okino, R. Dhami, A. Dagan, S. Gatt, H. Schulze, K. Sandhoff, and E. H. Schuchman, Purification and Characterization of Recombinant, Human Acid Ceramidase, J. Biol. Chem., 278, pp32978-32986, 2003.08.
Works, Software and Database
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1. Nozomu Okino, Makoto Ito, Transcriptional regulation of Pseudomonas ceramidase through the sphingosine-specific transcriptional regulator SphR
, 58th International Conference on the Bioscience of Lipids, 2017.09.
2. 沖野 望, Yoshimitsu Kakuta, 鵜木(加藤)陽子, 大坪 怜央, 石橋 洋平, 小林 宇太郎, makoto kimura, 伊東 信, Crystal structures of unique glycosphingolipid-degrading enzymes, The 7thACGG conference, 2015.11.
3. 沖野 望, 伊東 信, Marine biotechnology: New understanding and developments, PKNU – KU Joint Symposium SMART Oceans and Fisheries, 2015.10.
4. 沖野 望, 伊東 信, Identification of a transcriptional regulator SphR for ceramidase expression in Pseudomonas aeruginosa, 第10回スフィンゴテラピィ研究会, 2015.06.
Educational Activities
Faculty of Agriculture
(Animal Production Science)

Marine Resource Chemistry
Science English I,II

Graduate School
(Bioscience and Biotechnology)
Advanced Marine Biochemistry
Molecular Biosciences