Kyushu University [keiji yasukawa (Assistant Professor) Faculty of Pharmaceutical Sciences, Department of Chemo-Pharmaceutical Sciences]

Department of Chemo-Pharmaceutical Sciences
Graduate School of Pharmaceutical Sciences

Department of General Pharmaceutical Sciences
School of Pharmaceutical Sciences

Ph.D.

Biophysics

Contents of my research are followings:
1) Research on in vivo redox change associated with on-set/development of gastrointestinal diseases

It is almost clarified that the unbalance of redox balance between production of reactive oxygen species and free radicals and engoneous antioxidants is closely associated with on-set/development of various diseases. It is useful to combine the redox imager such as in vivo ESR and OMRI with spin probe technique in order to measure in vivo redox status non-invasibly. The relationship between the redox balance and the disease has been investigating in the experimental models of gastric ulcer, ulcerative colitis, and small intestinal inflammation using this combined technique.

2) Development of image processing software for redox imager such as ESRI, ESRI/OMRI, and OMRI

Because image quality of ESRI and the multimodality (ESRI/MRI) is influenced by irregular noise followed by real-time measurement in organisms, the optimum filter in the image process was investigated using phantom and tested for animal study. The software making the map of probe metabolism rate from raw OMRI data was developed. Moreover, the development of 3-dimensional imaging software fused between redox and oxygen concentration, which are obtainable from OMRI.

3) Research on bioinformatics such as bioassay data

The software of curve-fitting based on the logistic formula for bioassay data of a few hundred of chemicals and of making matrix data of parameters-bioassays in each chemical.

4) Research on reactivity between nitroxyl radicals and reactive oxygen species/reactive nitrogen species

It is investigated that reactivity between nitroxyl radical and hydroxyl radical, which is most hazardous to biomolecules, superoxide, and nitric oxide is investigated using ESR spectroscopy.

Research Interests

Research on in vivo redox change associated with on-set/development of gastrointestinal diseases
keyword : gastric ulcer, ulcerative colitis, small intestinal inflammation, free radical, electron spin resonance, nitroxyl radical
1998.04.
Development of imaging processing software for redox imager
keyword : electron spin resonance, proton magnetic resonance imaging, nitroxyl radical
2003.10.
Research on bioinformatics such as bioassay data
keyword : gastric ulcer, free radical, electron spin resonance, nitroxyl radical
2006.04.
Study on reactivity of nitroxyl radicals with reactive oxygen/nitrogen species
keyword : nitroxyl radical, reactive oxygen species, nitric oxide
2003.10.

Current and Past Project

JSPS Core-to-Core Program
Coordinator in Japan is Dr. Keiji Yasukawa.
Core Institute is Kyushu University.
Title of the proposal; Center for Magnetic Resonance Molecular Imaging for In vivo Redox System
Period; 2009/04/01 – 2012/03/31
JSPS Core-to-Core Program "Integrated Action Initiatives"
Coordinator in Japan is Prof. Hideo UTSUMI.
Core Institute is Kyushu University.
Title of the proposal; Center for Magnetic Resonance Molecular Imaging for In vivo Redox System
Period; 2007/04/23 – 2009/03/31
JSPS Asia Core Program
Establishment of a Research and Education Center for Hematopoietic Disorders in Asia

Academic Activities

Books

Show All Books >>

1.	K. Yasukawa, K. Yamada, K. Ichikawa, and H. Utsumi, Methods in Redox Signaling,Mary Ann Liebert, Inc.,pp. 90-99., In vivo ESR/Spin Probe Technique.,2010.01.
2.	Title : Approach for electron spin science and spin technology Authors : K.Yasukawa, F.Hyodo and H.Utsumi Publishing office : Nippon Gakkai Zimu Center.
3.	K.Yasukawa and H.Utsumi,EPR in the 21st century: basics and applications to materials, life and earth sciences,ELSEVIER,pp 548-551,2002.01.

Reports

Show All Reports >>

1.	Organizing Committee of Winter School in Fukuoka (Keiji Yasukawa, Fuminori Hyodo),Abstract of 16th JSPS Core-to-Core Seminar: Winter School in Fukuoka,ミドリ印刷,2011.01.
2.	Report of exchange program for young researchres (FY2008) JSPS Core-to-Core Program "Center for Magnetic Resonance Molecular Imaging of In Vivo Redox System" Coordinator in Japan side:Hideo Utsumi, secretary general:Keiji Yasukawa.
3.	Title:Molecular Imaging of in vivo Redox Dynamics using Magnetic Resonance System Authors: Hideo Utsumi and Keiji Yasukawa Journal:"Function & Materials", CMC Books, July 2008..
4.	Report of exchange program for young researchres (FY2007) JSPS Core-to-Core Program "Center for Magnetic Resonance Molecular Imaging of In Vivo Redox System" Coordinator in Japan side:Hideo Utsumi, secretary general:Keiji Yasukawa.

Papers

Show All Papers >>

1.	keiji yasukawa, Hiroto Tokuda, Xin Tun, Hideo Utsumi, Ken-ichi yamada,The detrimental effect of nitric oxide on tissue is associated with infl ammatory events in the vascular endothelium and neutrophils in mice with dextran sodium sulfate-induced colitis.,Free Radical Research,Vol.46,No.12,2012.12.
2.	Kosem N, Naganuma T, Ichikawa K, Phumala Morales N, Yasukawa K, Hyodo F, Yamada KI, Utsumi H.,Whole-body kinetic image of a redox probe in mice using Overhauser-enhanced MRI.,Free Radic Biol Med.,Epub ahead of print,2012.04.
3.	H. Deguchi, K. Yasukawa, T. Yamasaki, F. Mito, Y. Kinoshita, T. Naganuma, S. Sato, M. Yamato, K. Ichikawa, K. Sakai, H. Utsumi and K. Yamada.,Nitroxides prevent exacerbation of indomethacin-induced gastric damage in adjuvant arthritis rats,Free Radic. Biol. Med.,Vol.51,No.9,pp.1799-1805,2011.09.
4.	Kato TA, Monji A, Yasukawa K, Mizoguchi Y, Horikawa H, Seki Y, Hashioka S, Han YH, Kasai M, Sonoda N, Hirata E, Maeda Y, Inoguchi T, Utsumi H, Kanba S.,Aripiprazole inhibits superoxide generation from phorbol-myristate-acetate (PMA)-stimulated microglia in vitro: Implication for antioxidative psychotropic actions via microglia.,Schizophr Res,Vol.129,No.2-3,pp.172-182,2011.07.
5.	Araki S, Hirooka Y, Kishi T, Yasukawa K, Utsumi H, Sunagawa K.,Olmesartan reduces oxidative stress in the brain of stroke-prone spontaneously hypertensive rats assessed by an in vivo ESR method.,Hypertens Res,Sep 18. [Epub ahead of print],2009.09.
6.	Yasukawa K, Miyakawa R, Yao T, Tsuneyoshi M, Utsumi H.,Non-invasive monitoring of redox status in mice with dextran sodium sulphate-induced colitis.,Free Radical Research,43(5): 505-513,2009.03.
7.	Utsumi, H.; Yasukawa, K.; Soeda, T.; Yamada, K.; Shigemi, R.; Yao, T.; Tsuneyoshi, M.,Non-invasive mapping of reactive oxygen species by in vivo electron spin resonance spectroscopy in indomethacin-induced gastric ulcers in rats,Journal of Pharmacology and Experimental Therapeutics,317(1):228-35,2006.01.
8.	K.Yasukawa, K.Kasazaki, F.Hyodo and H.Utsumi,Non-invasive analysis of reactive oxygen species generated in rats with water immersion restraint-induced gastric lesions using in vivo electron spin resonance spectroscopy,Free Radical Research,Vol.38,No.2,Vol.38(2), pp147-155,2004.02.

Presentations

Show All Presentations >>

1.	Title: Redox Imaging of gastrointestinal diseases in experimental animals using Overhauser MRI. Author:Keiji Yasukawa, Kazuhiro Ichikawa, and Hideo Utsumi Name of Conference:The 7th Annual Meeting of The Japanese Gastroenterological Association Place:Kyoto Date: Feb 19, 2011.
2.	Noninvasive analysis of in vivo redox status involved in the initiation/development of gastric mucosal lesion in rats with in vivo ESR spectroscopy, Keiji Yasukawa, Tomomi Kanbe, Ryota Shigemi, Tetsuhiro Soeda, Keiko Kasazaki, Hideo Utsumi .
3.	Monitoring of in vivo redox status in rats with indomethacin-induced gastric lesion using Overhauser-enhanced MRI, Keiji Yasukawa,Tomomi Kanbe, Ryota Shigemi, Yao Takashi, Masazumi Tsuneyoshi and Hideo Utsumi, A Joint Conference of the 13th In Vivo ESR/EPR Spectroscopy & Imaging and the 10th International EPR Spin Trapping/Spin Labeling (EPR2008), JAL Resort Sea Hawk Hotel Fukuoka, Sep 28-30 (2008) .
4.	Title:Imaging analysis of in vivo redox reaction in rats with indomethacin-induced gastric lesion Authors: Keiji Yasukawa, Tomomi Kanbe, Ryota Shigemi, Takashi Yao, Masazumi Tsuneyoshi, Hideo Utsumi.
5.	Title:Imaging Analysis of Generation of Reactive Oxygen Species in Rats with Indomethacin- Induced Gastric Lesion Authors:Keiji Yasukawa,Tomomi Kanbe, Ryota Shigemi, Yao Takashi, Masazumi Tsuneyoshi and Hideo Utsumi May 25-27(2008), The Blackwell Inn, Columbus, OH, USA [URL].
6.	Title:Basic Study of Reactivity of Nitroxyl Radical Analogues and Nitric Oxide Authors:Keiji Yasukawa, Yusaku Mutsumoto, Hideo Utsumi Name:The 4th JSPS Core-to-Core Program Seminar:International In Vivo Redox Symposium Period:November 4-5, 2007 Place:Nihon-Daira Hotel, Shizuoka, Shimizu, Japan [URL].
7.	Title:Basic Study of Reactivity of Nitroxyl Radical Analogues and Nitric Oxide Authors:Keiji Yasukawa, Yusaku Mutsumoto, Hideo Utsumi Name:ISESS/SEST2007 Period:November 6-9, 2007 Place:Granship Shizuoka, Shizuoka, Japan.
8.	Title:Molecular Imaging of Free Radical Generation involved in rats with indomethacin-induced gastric lesion Authors:Yasukawa Keiji, Tomomi Kanbe, Ryota Shigemi, Tetsuhiro Soeda, Utsumi Hideo Name:5th JSPS Core-to-Core Program Seminar:Winter School on Redox Biology, Chemistry & Imaging Period:November 12, 2007 Place:NIH/NCI Building 10, Bethesda, MD [URL].
9.	Title:IMAGING ANALYSIS OF GENERATION OF REACTIVE OXYGEN SPECIES IN RATS WITH INDOMETHACIN-INDUCED GASTRIC LESION Authors:Keiji YASUKAWA, Tomomi KANBE, Ryota SHIGEMI, Tetsuhiro SOEDA, Kazuhiro ICHIKAWA, Kenichi YAMADA and Hideo UTSUMI Name:SFRBM2007 Period:November 14-18, 2007 Place:Washington DC, USA.
10.	NON-INVASIVE ANALYSIS OF REACTIVE OXYGEN SPECIES GENERATION USING IN VIVO ELECTRON SPIN RESONANCE SPECTROSCOPY IN MICE WITH DEXTRAN SODIUM SULFATE-INDUCED COLITIS, Keiji YASUKAWA, Reiko MIYAKAWA, and Hideo UTSUMI, EPR 2007: A Joint Conference of the 12th In Vivo EPR Spectroscopy and Imaging and the 9th International EPR Spin Trapping/Spin Labeling, Hilton Suites Chicago/Magnificent Mile, Chicago, USA, April 29-May 3, 2007 .
11.	Reaction of nitroxyl radical derivatives with nitric oxide, Keiji YASUKAWA, Yusaku MUTSUMOTO, and Hideo UTSUMI, The 7th Annual Scientific Meeting of the NO Society of Japan, Shiga, Japan, May 17-18, 2007 .
12.	Title : Non-invasive analysis of reactive oxygen species generation using in vivo electron spin resonance spectroscopy in mice with dextran sodium sulfate-induced colitis Authors : K. Yasukawa, R. Miyakawa, H. Utsumi Name : 13th Society of Free Radical Biology and Medicine (SFRBM2006) Location and Date : Denver, Colorado, USA(November 16th-19th, 2006).
13.	Title : Molecular imaging of in vivo redox and its application to evaluation of drug medicines and medicinal plants Authors : K. Ichikawa, K. Yasukawa, T. Naganuma, H. Utsumi Name : Tentative Program JSPS Asia Core Program Kyushu-Mahidol Universities Location and Date : Bangkok, Thailand (August 8th-10th, 2006).
14.	Title : Image analysis of reactive oxygen species generation using OMRI in indomethacin-induced gastric ulcers in rats Authors : K. Yasukawa, R. Sigemi, T. Soeda, K. Yamada, H. Utsumi Name : 15th workshop on digestive diseases and free radicals Location and Date : Kyoto (February 25th, 2006).
15.	Title : Image analysis of reactive oxygen species generation using in vivo ESR spectroscopy and PEDRI in indomethacin-induced gastric ulcers in rats Authors : K. Yasukawa, T. Soeda, R. Sigemi, K. Yamada, S. Matsumoto, H. Utsumi Name : 12th Society of Free Radical Biology and Medicine (SFRBM) Location and Date : Austin, Texas , United States of America (November 17th~20th, 2005).
16.	Title: Image analysis of reactive oxygen species generated in the initiation and the progression of DSS-induced colitis. Authors: K. Yasukawa, R. Miyakawa, K. Okuma and H. Utsumi Name: The 44th society of electron spin science and spin technology Location and Date: Sendai, JAPAN (24th October - 26th October, 2005).
17.	Title: Evaluation of gastric ulcer formation using in vivo ESR/spin probe technique Authors: K. Yasukawa, K. Kasazaki and H. Utsumi Name: Pharmaceutical Sciences World Congress(PSWC)2004 Location and Date: Kyoto, JAPAN (29th May-3rd June, 2004).
18.	Title : Evaluation of gastric ulcer formation using in vivo ESR/spin probe technique Authors : K.Yasukawa, K.Kasazaki and H.Utsumi Name : International symposium on the instrumentation of EPR spectroscopy Location and Date : Yamagata, JAPAN (December 3rd-4th, 2003).
19.	Title : Evaluation of gastric ulcer formation using in vivo ESR/spin probe technique Authors : K.Yasukawa, K.Kasazaki and H.Utsumi Name : The 20th pharmaceutical society of Japan in Kyushu branch Location and Date : Fukuoka, JAPAN (November, 2003).
20.	Title : Anti-oxidative activity of nitroxyl radical in gastric mucosal lesion in rats Authors : K. Yasukawa, T. Soeda, R. Shigemi and H. Utsumi Name : Fifth AFMC International Medicinal Chemistry Symposium Location and Date : Kyoto Park Hotel (October 14th~17th, 2003).
21.	Title : Basic study on the reaction of hydroxyl radical with a nitroxyl spin probe Authors : F. Oda, K. Yasukawa and H. Utsumi Name : The 10th International Workshop on Bio-Medical ESR Spectroscopy and Imaging Location and Date : Yamanoue Hotel, Fukuoka, Japan (April 1st~3rd, 2003).
22.	Title : Non-invasive analysis of in vivo free radical reaction in NSAIDs-induced gastric lesion model rats Authors : K. Yasukawa, T. Soeda and H. Utsumi Name : The 10th International Workshop on Bio-Medical ESR Spectroscopy and Imaging Location and Date : Yamanoue Hotel, Fukuoka, Japan (April 1st~3rd, 2003).
23.	Title : Non-invasive analysis of in vivo free radical reactions involved in the formation of gastric mucosal lesions induced by NSAIDs Authors : K.Yasukawa, T.Soeda and H.Utsumi Name : The 12th workshop on digestive diseases and free radicals Location and Date : Kyoto, JAPAN (February 15th, 2003).
24.	Title : In vivo ESR analysis of stress-induced gastric ulcer in rats Authors: K. Yasukawa, K. Kasazaki, H. Sano and H. Utsumi Name : International Workshop on Techniques and Bio-Medical Applications of In Vivo EPR and PEDRI Location and Date : University of Aberdeen, Scotland, England(September 12th~17th, 1999).

Membership in Academic Society

The Pharmaceutical Society of Japan
Society for Free Radical Research ,Japan
The Society of Electron Spin Science and Technology
NO Society of Japan
Japanese Society of Magnetic Resonance in Medicine

Professional and Outreach Activities

1) JSPS Core-to-Core Program
"Center for Magnetic Resonance Molecular Imaging of In Vivo Redox System"

2) JSPS Asia Core Program
“Establishment of a Research and Education Center for Hematopoietic Disorders in Asia”
Research title: Study on oxidative diseases and antioxidant properties of plant extracts in Thailand

[URL]This URL is the HP of Laboratory of Bio-function Science, Graduate School of Pharmaceutical Sciences..

[URL]
This URL is the HP of Laboratory of Bio-function Science, Graduate School of Pharmaceutical Sciences..