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Naohisa Wada Last modified date：2023.10.06

Professor / Division of Interdisciplinary Dentistry
Department of Dental Science
Faculty of Dental Science


Graduate School
Department of Dental Science
Graduate School of Dental Science
Department of Dental Science
Graduate School of Dental Science
Undergraduate School
Department of Dentistry
School of Dentistry
Other Organization
Research Center for Education in Health Care System
Clinical Education Center
Health　Networking　Center
Comprehensive Dentistry


Homepage
https://kyushu-u.elsevierpure.com/en/persons/naohisa-wada
　Reseacher Profiling Tool　Kyushu University Pure
http://www.dent.kyushu-u.ac.jp/dh/
Kyushu University Hospital Dental Center .
Phone
092-642-6490
Academic Degree
Ph.D
Country of degree conferring institution (Overseas)
No
Field of Specialization
General Dentistry (Endodontology, Operative Dentistry, Periodontology)
Total Priod of education and research career in the foreign country
02years07months
Outline Activities
research: To achieve regeneration of periodontal ligament, I study about chracterization of periodontal ligament and searching the specific factor for periodontal ligament.
Research
Research Interests
  • Establishing novel method to regenerate dentin-dental pulp complex using stem cells, signal molecules and scaffolds.
    keyword : dental pulp, dentin, stem cell, tissure engineering
    2013.04.
  • Identification of novel growth factor and extracellular matrix protein which are specific in periodontal ligament stem cell population
    keyword : Periodontal ligament stem cell
    2010.04.
  • Reprogramming of human gingival fibroblasts and periodontal ligament cells to pluripotency with defined factors
    keyword : gingival cell, periodontal ligament cell, iPS
    2008.04～2009.03.
  • Immunosuppressive mechanisms of allogeneic periodontal ligament stem cells on T-lymphocytes
    keyword : periodontal ligament stem cell, T-lymophocyte, allogeneic transplantation
    2007.09～2010.03.
  • Identification of novel factors to induce periodontal tissue regeneration
    keyword : periodontal ligament
    2006.04.
  • Establishment and characterisation of human periodontal ligament cell clone and epithelial cell clone derived from human epithelial rests of Malassez
    keyword : periodontal ligament, cell clone
    2003.04～2006.03.
  • Lipopolysaccharide stimulates expression of osteoprotegerin and receptor activator of NF-kappa B ligand in periodontal ligament fibroblasts through the induction of interleukin-1 beta and tumor necrosis factor-alpha
    keyword : periodontal ligament, osteoclast, LPS
    2001.04～2003.03.
  • The study of the mechanism(s) and the factor for periodontal ligament cells to regulate the osteoclast differentiation and function
    keyword : periodontal ligament, osteoclast
    1998.04～2002.03.
  • Apoptosis of epithelial cells proliferated in human periapical lesions
    keyword : periapical granuloma, periapical cyst, apoptosis
    1997.04～1998.03.
Academic Activities
Books
 Show All Books >>
1. Naohisa Wada, Atsushi Tomokiyo, Hidefumi Maeda, Dental Stem Cells, Springer International Publishing Switzerland, 2016.05.
2. Hidefumi Maeda, Naohisa Wada, Fujii Shinsuke, Atsushi Tomokiyo, Akifumi Akamine, Periodontal ligament stem cells. In: Stem Cells in Clinic and Research, InTech, Rijeka, Croatia, Chapter 25, 619-636, 2011.07.
Reports
 Show All Reports >>
1. Naohisa wada, Stan Gronthos, P.Mark Bartold, Immunomodulatory Effects of Stem Cells, Periodontol 2000. 63(1):198-216., 63(1):198-216, 2013.10.
Papers
 Show All Papers >>
1. Naati Fakatava, Hiromi Mitarai, Asuka Yuda, Akira Haraguchi, Hiroko Wada, Daigaku Hasegawa, Hidefumi Maeda, Naohisa Wada, Actin alpha 2, smooth muscle, a transforming growth factor-β1-induced factor, regulates collagen production in human periodontal ligament cells via Smad2/3 pathway., Journal of dental sciences, 10.1016/j.jds.2022.08.030, 18, 2, 567-576, 2023.04, BACKGROUND/PURPOSE: Actin alpha 2, smooth muscle (ACTA2) is an actin isoform that forms the cytoskeleton. Actin plays a crucial role in numerous cellular functions. ACTA2 is a marker of functional periodontal ligament (PDL) fibroblasts and is upregulated by transforming growth factor-β1 (TGF-β1); however, the underlying function of ACTA2 in PDL tissue is unknown. We aimed to examine the localization and potential function of ACTA2 in PDL tissues and cells. MATERIALS AND METHODS: RNA expression was determined using semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) and quantitative RT-PCR. Protein expression was determined using immunofluorescence staining and Western blot analysis. Soluble and insoluble collagen production was examined using the Sircol collagen assay and picrosirius red staining, respectively. Small interfering RNA (siRNA) was used for knockdown assay to examine the effect of ACTA2 in human PDL cells. RESULTS: ACTA2 expression was observed in human primary PDL cells and PDL cell line (2-23 cells). TGF-β1 upregulated ACTA2, collagen type Ⅰ alpha1 chain (COL1A1), periostin (POSTN), and fibrillin-Ⅰ(FBN1) expression and soluble and insoluble collagen production in 2-23 cells. However, ACTA2 depletion by siRNA strongly suppressed PDL-related gene expression and collagen production compared with those of TGF-β1-stimulated control cells. Furthermore, ACTA2 knockdown significantly suppressed the phosphorylation of Smad2 and Smad3. CONCLUSION: ACTA2 plays a crucial role in PDL-related marker expression and collagen production via Smad2/3 phosphorylation. Our findings might contribute to the development of novel and effective periodontal therapies..
2. Shinsuke Fujii, Kengo Nagata, Shinji Matsumoto, Kenichi Kouhashi, Akira Kikuchi, Yoshinao Oda, Tamotsu Kiyoshima, Naohisa Wada, Wnt/β-catenin signaling, which is activated in odontomas, reduces Sema3A expression to regulate odontogenic epithelial cell proliferation and tooth germ development, Scientific reports, 10.1038/s41598-019-39686-1, 9, 1, 2019.12.
3. Daigaku Hasegawa, Naohisa Wada, Shinichiro Yoshida, Hiromi Mitarai, Mai Arima, Atsushi Tomokiyo, Sayuri Hamano, Hideki Sugii, Hidefumi Maeda, Wnt5a suppresses osteoblastic differentiation of human periodontal ligament stem cell-like cells via Ror2/JNK signaling, Journal of Cellular Physiology, 10.1002/jcp.26086, 233, 2, 1752-1762, 2018.02.
4. H. Mitarai, Naohisa Wada, Daigaku Hasegawa, Shinichiro Yoshida, M. Sonoda, Atsushi Tomokiyo, Sayuri Hamano, S. Serita, H. Mizumachi, Hidefumi Maeda, Transgelin mediates transforming growth factor-β1-induced proliferation of human periodontal ligament cells, Journal of Periodontal Research, 10.1111/jre.12466, 52, 6, 984-993, 2017.12.
5. Shinichiro Yoshida, Naohisa Wada, Daigaku Hasegawa, H. Miyaji, H. Mitarai, Atsushi Tomokiyo, Sayuri Hamano, Hidefumi Maeda, Semaphorin 3A Induces Odontoblastic Phenotype in Dental Pulp Stem Cells., J Dent Res, 10.1177/0022034516653085, 95, 11, 1282-1290, 2016.06.
6. Daigaku Hasegawa, Naohisa Wada, Hidefumi Maeda, S.Yoshida, H. Mitarai, Atsushi Tomokiyo, Monnouchi Satoshi, S. Hamano, A. Yuda, Akifumi Akamine, Wnt5a Induces Collagen Production by Human Periodontal Ligament Cells through TGFβ1-mediated Upregulation of Periostin Expression., J Cell Physiol, 230, 11, 2647-2660, 2015.11.
7. Zakaria MN, Toru Takeshita, Yukie Shibata, Hidefumi Maeda, Naohisa Wada, Akifumi Akamine, Yoshihisa Yamashita, Microbial community in persistent apical periodontitis: a 16S rRNA gene clone library analysis., Int Endod J, in press, 2014.08.
8. Menicanin D, Mrozik M, Naohisa Wada, Marino V, Shi S, Bartold PM, Gronthos S., Periodontal Ligament Derived Stem Cells Exhibit the Capacity for Long-Term Survival, Self-Renewal and Regeneration of Multiple Tissue Types in Vivo. , Stem Cells Dev, 23, 9, 1001-1011, 2014.05.
9. Naohisa Wada, Hidefumi Maeda, Hasegawa D, Gronthos S, Bartold PM, Menicanin D, Fujii S, Yoshida D, Tomokiyo A, Monnouchi Satoshi, Akifumi Akamine, Semaphorin 3A induces mesenchymal stem-like properties in human periodontal ligament cells., Stem Cells Dev, in press, 2014.02.
10. Mrozik K, Naohisa Wada, Marino V, Richter W, Shi S, Wheeler DL, Gronthos S, Bartold PM, Regeneration of periodontal tissues using allogeneic periodontal ligament stem cells in an ovine model., Regen Med, 8, 6, 711-723, 2013.06.
11. Wada N, Wang B, Lin NH, Laslett AL, Gronthos S, Bartold PM, Induced pluripotent stem cell lines derived from human gingival fibroblasts and periodontal ligament fibroblasts., J Periodontal Res, 46, 4, 438-447, 2011.05.
12. Wada N, Bartold PM, Gronthos S, Human foreskin fibroblasts exert immunomodulatory properties by a different mechanism to bone marrow mesenchymal stem cells, Stem Cells Dev, 20, 4, 647-659, 2010.09.
13. Wada N, Menicanin D, Shi S, Bartold PM, Gronthos S, Immunomodulatory properties of human periodontal ligament stem cells, J Cell Physiol, 219, 667-676, 2009.01.
14. Tomokiyo A, Maeda H, Fujii S, Wada N, Shima K, Akamine A, Development of a multipotent clonal human periodontal ligament cell line. Differentiation, Differentiation, 76, 4, 337-347, 2008.04.
15. Fujii S, Maeda H, Wada N, Tomokiyo A, Saito M, Akamine A., Investigating a clonal human periodontal ligament progenitor/stem cell line in vitro and in vivo, J Cell Physiol, 215, 3, 743-749, 2008.03.
16. Fujii S, Maeda H, Wada N, Kano Y, Akamine A, Establishing and characterizing human periodontal ligament fibroblasts immortalized by SV40T-antigen and hTERT gene transfer., Cell and Tissue Research, vol.324(1):pp117-125, 2006.04.
17. Nomiyama H, Egami K, Wada N, Tou K, Horiuchi M, Matsusaki H, Miura R, Yoshie O, Kukita T, Identification of genes differentially expressed in osteoclast-like cells., Journal of Interferon and Cytokine Research, 10.1089/jir.2005.25.227, 25, 4, 227-231, vol.25: pp227-231, 2005.01.
18. Kukita T, Wada N, Kukita A, Kakimoto T, Sandra F, Toh K, Nagata K, Iijima T, Horiuchi M, Matsusaki H, Hieshima K, Yoshie O, Nomiyama H., RANKL-induced DC-STAMP is essential for osteoclastogenesis., Journal of Experimental Medicine, 10.1084/jem.20040518, 200, 7, 941-946, vol.200: pp941-946, 2004.06.
19. Wada N, Maeda H, Yoshimine Y, Akamine A, Lipopolysaccharide stimulates expression of osteoprotegerin and receptor activator of NF-kappa B ligand in periodontal ligament fibroblasts through the induction of interleukin-1 beta and tumor necrosis factor-alpha., Bone, 10.1016/j.bone.2004.04.023, 35, 3, 629-635, vol.35: pp629-635, 2004.04.
20. Wada N, Maeda H, Tanabe K, Tsuda E, Yano K, Nakamuta H, Akamine A, Periodontal ligament cells secrete the factor that inhibits osteoclastic differentiation and function: the factor is osteoprotegerin / osteoclastogenesis inhibitory factor., Journal of Periodontal Research, 10.1034/j.1600-0765.2001.00604.x, 36, 1, 56-63, vol.36: pp56-63, 2001.01.
Presentations
 Show All Presentations >>
1. Hiromi Mitarai, Naohisa Wada, Daigaku Hasegawa, Shinichiro Yoshida, Mai Sonoda, Atsushi Tomokiyo, Sayuri Hamano, Hidefumi Maeda, Transgelin Mediates TGF-beta1-induced Human Periodontal Ligament Cell Proliferation., 95th General Session & Exhibition of the International Association for Dental Research (IADR), 2017.03.
2. Daigaku Hasegawa, Naohisa Wada, Sayuri Hamano, Atsushi Tomokiyo, Shinichiro Yoshida, Hiromi Mitarai, Mai Sonoda, Hideki Sugii, Hidefumi Maeda, Identification of a novel periodontal ligament stem cell marker, 94th General Session & Exhibition of the International Association for Dental Research (IADR), 2016.06.
3. Shinichiro Yoshida, Naohisa Wada, Daigaku Hasegawa, Atsushi Tomokiyo, Sayuri Hamano, Hiromi Mitarai, Hideki Sugii, Hidefumi Maeda, Semaphorin 3A induces odontoblastic phenotype in dental pulp stem cells., 94th General Session & Exhibition of the International Association for Dental Research (IADR), 2016.06.
4. Sayuri Hamano, Atsushi Tomokiyo, Naohisa Wada, Daigaku Hasegawa, Hideki Sugii, Shinichiro Yoshida, Suguru Serita, Hiroyuki Mizumachi, Hiromi Mitarai, Hidefumi Maeda, Directing human iPS cells toward PDL stem cells, 94th General Session & Exhibition of the International Association for Dental Research (IADR), 2016.06.
5. Shinichiro Yoshida, Naohisa Wada, Daigaku Hasegawa, Atsushi Tomokiyo, Sayuri Hamano, Hiromi Mitarai, Serita S, Mizumachi H, Hidefumi Maeda, Semaphorin 3A induces odontoblastic phenotype in dental pulp stem cells., Kyudai Oral Bioscience 2016 -Frontiers in Dental Research and Education in East Asia-, 2016.02.
6. Daigaku Hasegawa, Naohisa Wada, Hidefumi Maeda, Shinichiro Yoshida, Monnouchi Satoshi, Koori Katsuaki, Sayuri Hamano, Hiromi Mitarai, Akifumi Akamine, The Effects of Wnt5a on Human Periodontal Ligament Cells., Kyudai Oral Bioscience 2014 - 8th International Symposium -, 2014.02.
7. Shinichiro Yoshida, naohisa wada, Hidefumi Maeda, Daigaku Hasegawa, Sato H, Monnouchi Satoshi, Akifumi Akamine, Dental pulp stem cells on bone tissue express periodontal ligament-related gene., The 9th World Endodontic Congress, 2013.05.
8. naohisa wada, Hidefumi Maeda, Daigaku Hasegawa, Stan Gronthos, P.Mark Bartold, Daniela Menicanin, Fujii Shinsuke, Hiroko Wada, Atsushi Tomokiyo, Monnouchi Satoshi, Akifumi Akamine, Conversion of periodontal ligament cells into multipotent stem-like cells, 91st General Session & Exhibition of the IADR, 2013.03.
9. naohisa wada, Hidefumi Maeda, Daigaku Hasegawa, Stan Gronthos, P.Mark Bartold, Daniela Menicanin, Fujii Shinsuke, Hiroko Wada, Atsushi Tomokiyo, Monnouchi Satoshi, Akifumi Akamine, Induction of stem/undifferentiated cells from human periodontal ligament cells by Sema3A, The Australian Health and Medical Research Congress 2012, 2012.11.
10. Immunosuppressive properties of human periodontal ligament stem cells.
Membership in Academic Society
  • Japanese society of regenerative medicine
Educational
Educational Activities
My educational activities are supervision and direction of Residents and students of 5th and 6th grade. Especially, general dental treatments, operative dentistry and endodontology are my specialty to teach young doctors.
Other Educational Activities
  • 2023.01.
  • 2023.01.
  • 2022.12.
  • 2022.03.
  • 2021.12.
  • 2021.11.
  • 2021.09.
  • 2019.12.
  • 2018.12.
  • 2018.06.
  • 2018.06.
  • 2018.05.
  • 2017.12.
  • 2017.11.
  • 2016.03.
  • 2015.12.
  • 2015.09.
  • 2015.09.
  • 2015.07.
  • 2015.03.
  • 2014.05.
  • 2011.11.
  • 2010.04.
  • 2010.08.
  • 2010.09.
  • 2007.05.
  • 2006.09.
  • 2006.06.
  • 2006.06.
  • 2005.10.


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