Kyushu University Academic Staff Educational and Research Activities Database
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Yuichi Tsukada Last modified date:2018.06.23

Professor / Division of Medical Molecular Cell Biology, Graduate School of Systems Life Sciences
Advanced Biological Information Research Division
Inamori Frontier Research Center

Graduate School
Other Organization

Academic Degree
Country of degree conferring institution (Overseas)
Field of Specialization
Life Science
Total Priod of education and research career in the foreign country
Outline Activities
・Epigenetic regulation of gene expression
・Molecular basis of totipotency
Research Interests
  • Molecular basis of totipotency
    keyword : totipotency, genome reprogramming, early development
  • Epigenetic regulation of gene expression
    keyword : epigenetics, epigenome, methylation, early development
Academic Activities
1. Kaneda, A., Tsukada, Y. (Editor), DNA and Histone Methylation as Cancer Targets, Springer Nature, 2017.09, Epigenetics is a novel dogma that regulates gene expression profiles in the cells and
is known to be involved in cancer. Among numerous epigenetic factors, DNA and
histone methylation has a unique feature that this covalent modification is stable
compared to other covalent modifications, thereby its reversibility had been
unknown until recently. The significant advance of epigenetics has also occurred in
methylation. Histone methylation was revealed to be reversible by the discoveries of
histone demethylases, and DNA demethylation pathway was elucidated by the discovery
of DNA oxidases.
This book focuses on DNA and histone methylation in epigenetics and describes
how it is involved in the molecular mechanisms responsible for the development of
cancer. Each chapter summarizes the current knowledge of the molecular basis of
DNA and histone methylation to explain its involvement in cancer, and describes
the features of DNA and histone methylation associated with a particular type of
cancer, the current diagnostic/therapeutic applications, and future directions of
study on DNA and histone methylation as cancer targets..
2. Yuichi Tsukada, Yujiang Geno Shi, Epigenetics 2nd edition "The discovery of histone demethylases", Cold Spring Harbor Press, pp13-15, 2015.02.
3. Yuichi Tsukada, Yujiang Geno Shi, Cold Spring Harbor Perspective in Biology "The discovery of histone demethylases", Cold Spring Harbor Laboratory Press, 2013.09.
4. Yu-ichi Tsukada, Keiichi I, Nakayama, Cold Spring Harbor Protocol "In vitro histone demethylase assay", Cold Spring Harbor Laboratory Press, 10, 2010.10.
5. Tsukada, Y., Zhang, Y., Methods "Purification of histone demethylases from HeLa cells", 40: 318-326, 2006.12.
1. Tsukada, Y., Hydroxylation mediates chromatin demethylation., J.Biochem., 2012.03.
1. Yuichi Tsukada, Tomohiko Akiyama, Keiichi Nakayama, Maternal TET3 is dispensable for embryonic development but is required for neonatal growth, Sci. Rep., 5, 15876, 2015.10.
2. Takayoshi Suzuki, Hiroki Ozasa, Yukihiro Itoh, Peng Zhan, Hideyuki Sawada, Koshiki Mino, Louise Walport, Rei Ohkubo, Akane Kawamura, Masato Yonezawa, Yuichi Tsukada, Anthony Tumber, Hidehiko Nakagawa, Makoto Hasegawa, Ryuzo Sasaki, Tamio Mizukami, Christopher J. Schofield, Naoki Miyata, Identification of the KDM2/7 histone lysine demethylase subfamily inhibitor and its antiproliferative activity, J Med Chem., 56, 18, 7222-7231, 2013.09.
3. Yuichi Tsukada, Tohru Ishitani, Keiichi Nakayama, KDM7 is a dual demethylase for histone H3 Lys 9 and Lys 27 and functions in brain development, GENES & DEVELOPMENT, 24, 5, 432-437, 2010.03.
4. Masaaki Nishiyama, 押川 清孝, Yuichi Tsukada, Nakagawa, T., Iemura, S., Natsume, T., Fan, Y., Kikuchi, A., Skoultchi, I. A., Keiichi Nakayama, CHD8 suppresses p53-mediated apoptosis during early embryogenesis., Nat. Cell Biol., 11: 172-82, 2009.02.
5. He, J., Kallin, E. M., Yuichi Tsukada, Zhang, Y., The H3K36 demethylase Jhdm1b/Kdm2b regulates cell proliferation and senescence through p15Ink4b., Nat. Struct. Mol. Biol., 15: 1169-75, 2008.11.
6. Shirako E, Hirayama N, Yuichi Tsukada, Tanaka T, Kitamura N, Up-regulation of p21CIP1 expression mediated by ERK-dependent and -independent pathways contributes to hepatocyte growth factor-induced inhibition of HepG2 hepatoma cell proliferation., J Cell Biochem., 104(1):176-88., 2008.05.
7. Maemura, M., Yoshimoto, A., Yuichi Tsukada, Morishita, Y., Miyazawa, K., Tanaka, T., Kitamura, N., Inhibitory effect of c-Met mutants on the formation of branching tubules by a porcine aortic endothelial cell line. , Cancer Sci. , 2006.12.
8. Yuichi Tsukada, JHDM2A, a JmjC-Containing H3K9 Demethylase, Facilitates Transcription Activation by Androgen Receptor, Cell, 2006.05.
9. Yuichi Tsukada, Histone demethylation by a family of JmjC domain-containing proteins, Nature, 2006.02.
10. Cao, R., Tsukada, Y., and Zhang, Y. , Role of Bmi-1 and Ring1A in H2A ubiquitylation and Hox gene silencing., Mol. Cell, 2005.12.
11. Han, J., Tsukada, Y., Hara, E., Kitamura, N., and Tanaka, T. , Hepatocyte growth factor induces redistribution of p21ClP1 and p27KlP1 through ERK-dependent p16INK4a up-regulation, leading to cell cycle arrest at G1 in HEPG2 hepatoma cells. , J. Biol. Chem. , 2005.09.
12. Yuichi Tsukada, Miyazawa, K., Kitamura, N., High Intensity ERK Signal Mediates Hepatocyte Growth Factor-induced Proliferation Inhibition of Human Hepatocellular Carcinoma Cell Line HepG2., J. Biol. Chem. , 2001.11.
13. Tsukada, Y., Tanaka T., Miyazawa, K., and Kitamura, N. , Involvement of down-regulation of Cdk2 activity in hepatocyte growth factor-induced cell cycle arrest at G1 in the human hepatocellular carcinoma cell line HepG2., J. Biochem. (Tokyo) , 2004.11.
1. Yuichi Tsukada, Toward Understanding the Molecular Basis of Totipotency in Mammals, West Lake Symposium on Epigenetics, 2018.04.
2. M. Maemura, Y. Nakajima, Y. Ohkawa, Y. Tsukada, Developmental potential of single blastomere derived from pre-implantation embryo in mouse, Consortium of Biological Science 2017, 2017.12.
3. Y. Nakajima, M. Gotoh, Y. Tsukada, Developmental potential of single mouse blastomere, World Congress of Reproductive Biology 2017, 2017.09.
4. Yuichi Tsukada, The role of zygotic 5mC oxidation in mouse development, International Symposium on Epigenome Dynamics and Regulation in Germ Cells, 2016.02.
5. Yuichi Tsukada, Chemical modifications of chromatin, 9th International Symposium on Nanomedicine, 2015.12.
6. 束田 裕一, Regulation of Major Satellite Transcripts in Mouse Zygotes, The 1st International Symposium of Chemistry and Biology of RNA Interference, 2014.11.
7. 束田 裕一, Epigenetic Mechanisms of Gene Regulation, Kyocera Professor Meeting, 2014.09.
8. 束田 裕一, Regulation Mechanism of DNA Oxidation in Mammalian Zygotes, 8th Annual Conference of Asia Epigenome Alliance and 2nd Taipei Epigenetics and Chromatin Meeting, 2013.11.
9. Yuichi Tsukada, A unique regulatory phase of chromatin modification in the early mammalian embryo, The Fukuoka International Symposium on Genomics & Epigenomics, 2013.09.
10. 束田 裕一, Regulation of chromatin demethylation, The 8th International Symposium of the Institute Network, 2013.06.
11. KDM7 is a dual demethylase for histone H3 lysines 9 and 27 and functions in
brain development.
  • A study of epigenetic regulation of gene expression