|Shinya Oki||Last modified date：2019.07.02|
Lab Home Page (Developmental Biology) .
Researchmap (Shinya Oki) .
ChIP-Atlas (An integrative and comprehensive database for published ChIP-seq data) .
SraTailor (A simple GUI software for processing ChIP-seq data) .
Ph.D in Medicine
Field of Specialization
Developmental biology, Bioinformatics
Research InterestsMembership in Academic Society
- Development of the tools for analyzing and exploiting the ChIP-seq data.
keyword : ChIP-seq, Data processing, Visualization
- Dissection of the role of Nodal signaling in early mouse development
keyword : Mouse, development, Nodal
- Dissecting the Role of Fgf Signaling During Gastrulation and Left-Right Axis Formation in Mouse Embryos
keyword : left-right asymmetry, nodal, node, lateral plate
2009.04～2011.03Situs-specific organogenesis in the mouse results from leftward fluid flow in the node cavity and subsequent left-sided expression of Nodal in lateral plate mesoderm (LPM). Nodal expression at the node is essential for the subsequent asymmetric Nodal expression in left LPM, but the precise role of Nodal produced at the node has remained unknown. We have now investigated how the Nodal signal is transferred from the node to LPM. Externally supplied Nodal protein did not signal to LPM, suggesting that the Nodal signal is transferred to LPM via an internal route rather than an external one. Transgenic rescue experiments showed that the Nodal coreceptor Cryptic is required only in LPM, not at the node, for asymmetric Nodal expression in LPM, indicating that the Nodal signal is not relayed indirectly between the node and LPM. Nodal interacts in vitro with sulfated glycosaminoglycans (GAGs), which are specifically localized to the basement membrane–like structure between the node and LPM in the mouse embryo. Inhibition of sulfated GAG biosynthesis prevents Nodal expression in LPM. These data suggest that Nodal produced at the node may travel directly to LPM through interaction with sulfated GAGs..
Works, Software and Database
|1.||An integrative and comprehensive database for visualizing and making use of public ChIP-seq data.
- Japanese Association for Medical Artificial Intelligence