| 1. |
Honda, M., Muta, A., Shimazaki, A., Akasaka, T., Yoshikuni, M., Shimasaki, Y., Oshima, Y., High concentrations of perfluorooctane sulofonate in mucus of tiger puffer fish Takifugu rubripes: a laboratory exposure study., Environmental Science and Polution Research, 25, 2, 1551-1558, 2018.01. |
| 2. |
Mercedes Maceren-Pates, Yasuhisa Kurita, Gaudioso Pates Jr., Michiyasu Yoshikuni, A model for germ cell developement in a fully segmented worm., Zoological Letters, 10.1186/s40851-015-0035-y, 1, Vol. 1, Article NO. 34., 2015.12, Introduction
Polychaetes are segmented marine worms with body segments separated by a complete or incomplete septum. In most polychaetes the whole body cavity is filled with gametes during the breeding season. Platynereis dumerilii (Pl. dumerilii), whic. |
| 3. |
Keisuke Yamano, Atsushi Fujiwara, Michiyasu Yoshikuni, Induced spawning in the sea cucumber Apostichopus japonicus by neuropeptide, cubifirin., Bulletin of Fisheries Research Agency , 40, 121-128, 2015.03, In hatcheries the induction of spawning in sea cucumbers has been typically carried out by regulation of rearing conditions such as temperature and light intensity. However, this method is relatively ineffective and the rate of spawning is unpredictable. . |
| 4. |
Michiyasu Yoshikuni, Keisuke Yamano, Atsushi Fujiwara, Akifumi Nakamura, In vitro induction of oocyte maturation in the Japanese sea cucumber Apostichopus japonicus by cubifrin and the developmental ability of the eggs, Fisheries Science, 10.1007/s12562-013-0665-y, on line, 2013.08, We describe the process of cubifrin-induced in vitro oocyte maturation in the Japanese sea cucumber Apostichopus japonicus, and we propose a procedure for practical hatchery application. Oocyte maturation occurred 40–50 min after stimulation of the ovaries by cubifrin. Concurrently, eggs were released from the ovarian tubules, initially by slow peristalsis, followed by active release after about 60 min. The first and second polar bodies were released from the eggs at 90–100 min and about 140 min, respectively. Cubifrin was more effective in inducing oocyte maturation at 1–100 nM than at 100 pM. Cubifrin at 1 pM–10 nM in rearing seawater had no effect on larval development. Delayed fertilization reduced the developmental ability of eggs. Broodstock could be maintained at 15–16 °C for up to about 50 days without affecting the developmental ability of eggs. Eggs matured in vitro and in vivo were similar in their developmental ability. In conclusion, the in vitro induction of oocyte maturation is effective in Japanese sea cucumber and represents a viable alternative to the induction of spawning by cubifrin injection.. |
| 5. |
Michiyasu Yoshikuni, Yasushi Shibata, Takashi Iwamatsu, Norio Suzuki, Graham Young, Kiyoshi Naruse, Yohsitaka Nagahama, An oocyte-specific astacin family protease, alveolin, is released from cortical granules to trigger egg envelope hardening during fertilization in medaka (Oryzias latipes)., Developmental Biology, 372, 2, 239-248, 2012.12. |
| 6. |
Atsushi Fujiwara, Keisuke Yamano, Shin-ichi Kato, Michiyasu Yoshikuni, Spawning induced by cubifrin in the Japanese common sea cucumber, Apostichopus japonicus., Fisheries Science, 76, 5, 795-801, 2010.05. |
| 7. |
Masatoshi Mita*, Michiyasu Yoshikuni*, Kaoru Ohno, Yasushi Shibata, Paul Prasanth Bindhu, Pitchayawasin Sutashinee, Minoru Isobe, Yoshitaka Nagahama, A relaxin-like peptide purified from radial nerves induces oocyte maturation and ovulation in the starfish., PNAS: Proceedings of the National Academy of Sciences of the United States of America., 106, 23, 9507-9512., 2009.06. |
| 8. |
Senthilkumaran B, Sudhakumari CC, Wang DS, Sreenivasulu G, Kobayashi T, Kobayashi HK, Yoshikuni M, Nagahama Y., Novel 3beta-hydroxysteroid dehydrogenases from gonads of the Nile tilapia: phylogenetic significance and expression during reproductive cycle., Molecular Cellular Endocrinology, 299巻2号146-152頁, 2009.02. |
| 9. |
Shinichi Kato, Saori Tsurumaru, Makoto Taga, Tomoki Yamane, Yasushi Shibata, Kaoru Ohno, Atushi Fujiwara, Keisuke Yamano and Michiyasu Yoshikuni, Neuronal peptides induce oocyte maturtion and gamete spawning of sea cucumber, Apostichopus japonicus., Developmental Biology, 326巻1号169-176頁, 2009.02. |
| 10. |
Masatoshi Mita, Kazutoshi Yamamoto, Michiyasu Yoshikuhni, Kaoru Ohno, Yoshitaka Nagahama, Preliminary study on the receptor of gonad-stimulating subustance (GSS) as a gonadotropin of stafish., General and Comparative Endocrinology, 153巻299-301頁, 2007.08. |
| 11. |
Yoshikuni M., Mita M., Ohno K., Pitchayawasin S., Isobe M., Nagahama Y., Starfish gonad-stimulating substance (GSS); the first identified invertebrate gonadotropin. , Frontiers in Molecular Endocrinology, P21, 2005.04. |
| 12. |
Hirai T., Matsubara H., Shibata Y., Yoshikuni M., Nagahama Y., Itakura R., Mita M., cDNA cloning of a nove member of the glycoprotein hormone receptor family from a sea urchin Hemicentratus pulcherrimus., 5th Congress of the Asia and Oceania Society for Comparative Endocrinology, P, 2004.04. |
| 13. |
Shibata Y., Ono K., Peter Thomas, Nagahama Y., Yoshikuni M., Multiple expressions of membrane progestin receptors in medaka (Olyzias latipes) ovary., 3rd International Meeting on Rapid Responses to Steroid Hormones, P20, 2003.04. |
| 14. |
Hirai T., Shibata Y., Matsuda M., Yoshikuni M., Nagahama Y., Glycoprotein hormone receptor family in medaka (Olyzias latipes)., 7th International Symposium on Reproductive Physiology of Fish, P87, 2003.04. |
| 15. |
Yoshikuni M., Sagegami R., Nagahama Y., Proteome analysis: A new approach to identify key proteins involved in acquisition of maturational competence and oocyte maturation of medaka oocytes., 7th International Symposium on Reproductive Physiology of Fish, P99, 2003.04. |
| 16. |
Shibata Y., Nagahama Y. Yoshikuni M., Astacin-like protease, alveolin, is released from cortical vesicles and induces the changes of egg envelope proteins during fertilization in medaka (Olyzias latipes)., 7th International Symposium on Reproductive Physiology of Fish, P94, 2003.04. |
| 17. |
Yoshikuni M., Sagegami R., Nagahama Y., Proteome analysis of medaka oocytes: Studies of acquisition of maturational competence and signal transduction of oocyte maturation., 4th Intercongress of the Asia and Oceania Society for Comparative Endocrinology, P50, 2002.04. |
| 18. |
Shibata Y., Nagahama Y., Yoshikuni M., Alveolin, an astacin- like protease, is exo- cytozed from cortical vesicle during fertilization in medaka (Oryzias latipes) eggs., 14th International Congress of Comparative Endocrinology, P184, 2001.04. |
| 19. |
Mita M., Hirai T., Oba Y., Yoshikuni M., Nagahama Y., Glycoprotein hormone- receptor family in ovaries of starfish Asterina pectinifera., 14th International Congress of Comparative Endocrinology, O20, 2001.04. |
| 20. |
Yoshikuni M., Sagegami R., Nagahama Y., Proteome analysis of medaka (Oryzias latipes) oocytes during the acquisition of maturational competence., 14th International Congress of Comparative Endocrinology, P169, 2001.04. |
| 21. |
Mita M., Yoshikuni M., Nagahama Y., Role of ecto-ATP diphosphohydrolase in ovarian follicle cells of starfish Asterina pectin ifera., 3rd International Symposium on the Molecular and Cellular Biology of the Egg and Embryo Extracellular Matrix, P20, 2000.04. |
| 22. |
Shibata Y., Iwamatsu T., Nagahama Y., Suzuki N., Yoshikuni M., Identification and cDNA cloning of Alveolin, an extracellular metalloproteinase, which induces chorion hardening of medaka (Oryzias latipes) eggs upon fertilization., 3rd International Symposium on the Molecular and Cellular Biology of the Egg and Embryo Extracellular Matrix, P15, 2000.04. |
| 23. |
Guan G., Tanaka M., Todo T., Young G., Yoshikuni M., Nagahama Y., Carbonyl reductase-like 20β-hydroxysteroid dehydrogenases in the ovarian follicle of a teleost fish, the rainbow trout (Oncorhynchus mykiss): Their structures and reproductive functions in oocyte maturation., 6th Symposium of Reproductive Physiology of Fish, P70, 1999.04. |
| 24. |
Yoshikuni M., Oba Y., Nagahama Y., Purification of maturation-inducing hormone, 17a20b-dihydroxy-4-pregnen-3-one, receptor solubilized from isolated cortices of goldfish (Caracius auratus) oocytes., 6th Symposium of Reproductive Physiology of Fish, P191, 1999.04. |
| 25. |
Yamaguchi T., Shimizu F., Hirai T., Yoshikuni M., Nagahama Y., Saneyoshi M., Photo affinity labeling of the primer binding domain in HIV-1 reverse transcriptase with an oligodeoxyribonuleotide containing a phenyldiazirine derivative., Nucleic Acids Symposium Series, 39, 47-48, 1998.04. |
| 26. |
Yoshikuni M., Oba Y., Nagahama Y., A pertussis toxin sensitive GTP-binding protein is involved in the signal transduction pathway of the maturation-inducing hormone (17α，20β-dihydroxy-4- pregnen-3-one)., 1st International Meeting of Rap- id responses to Steroid Hormones, P58, 1998.04. |
| 27. |
Todo T., Kobayashi T., Kobayashi H., Suzuki K., Yoshikuni M., Yamauchi K., Nagahama Y. , Molecular cloning an characterization of a cDNA encoding nuclear progesterone receptor of the Japanese eel, Anguila japonica., 3rd International Symposium on Fish Endocrinology, P162, 1996.04. |
| 28. |
Jiang J.Q., Yamashita M., Yoshikuni M., Fukada S., Nagahama Y., Organization of cytoplasmic microtubules during 17α，20β-DP induced meiotic maturation of oocytes in goldfish(Carassius auratus)., 3rd International Symposium on Fish Endocrinology, P173, 1996.04. |
| 29. |
Yoshikuni M., Oba Y., Nagahama Y., A pertussis toxin sensitive GTP-binding protein is involved in the signal transduction pathway of the maturation-inducing hormone (17α,20β-dihydroxy-4-pregnen-3-one) of rainbow trout (Oncorhynchus mykiss) oocytes., 5th International Symposium of Reproductive Physiology of Fish, P348, 1995.04. |
| 30. |
Yoshikuni M., Nagahama Y., Specific bindings of [3H]17α,20β-dihydroxy-4-pregnen-3-one to oocyte cortices and plasma of rainbow trout (Oncorhynchus mykiss), 2nd International Symposium on Fish Endocrinology, L13, 1992.04. |
