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Etsuro Ono Last modified date:2019.08.15

Professor / Graduate School of medical sciences, Department of Basic Medicine, Department of Physiological Sciences, Department of Biomedicine
Department of Basic Medicine
Faculty of Medical Sciences


Graduate School
Other Organization
Other
Other
Other


E-Mail
Phone
092-642-6147
Fax
092-642-6165
Academic Degree
Ph.D.
Field of Specialization
Laboratory animal science, Virology
Research
Research Interests
  • Development of Disease-resistant Animals
    keyword : Disease-resistant Animals, Disease-resistant genes, Soluble form of viral receptor, intracellular immunization, Transgenic animal
    1990.06Generation of infectious disease-resistant animals Transgenic mice expressing resistant genes against pseudorabies have been generated for the development of livestock with enhanced resistance to pseudorabies. Nectin-1 is an alphaherpesvirus receptor that binds to virion glycoprotein D (gD). In order to assess the antiviral potential of an ectopic expression of the nectin-1 ectodomain in vivo, transgenic mouse lines possessing the chimeric gene expressing a fusion protein consisting of an extracellular domain of porcine nectin-1 and the Fc portion of human IgG1 were generated and showed a marked resistance to PRV infection..
  • Development of Animal Models for Virus-Induced Neurological Disorders
    keyword : Neurovirus, Neuropathogenecity, Transcription factor, Transgenic mice
    1995.05Generation and analysis of transgenic mice expressing transcription factors of neurotropic viruses Pseudorabies virus (PRV) infection in animals other than its natural host almost always gives rise to fatal diseases in the central nervous system as a result of infection of peripheral neurons and subsequent to the brain. To study the role of immediate-early protein (IE180) of PRV in neuropathogenicity, we have generated transgenic mouse lines expressing IE180 in a tetracycline-regulated system. The transgenic mice showed cerebellar symptoms such as ataxic gait, tremor and motor discoordination. In the transgenic mice, histopathology of the cerebellum was remarkable for a failure of layer formation and reduced cerebellum sizes. These findings suggest that IE180 affects the cascade of gene expression for development of the murine cerebellum. Borna disease virus infection causes a spectrum of behavioral deficits. To study the role of p24 of BDV in neuropathogenicity, we have generated transgenic mouse lines expressing p24 in astrocytes. The transgenic mice showed enhanced intermale aggressiveness and abnormalities in spatial learning. In the transgenic mice, brain-derived neurotrophic factor and synaptophysin were markedly decreased in hippocampus and cerebellum. These findings suggest that expression and accumulation of p24 in astrocytes creates neurodevelopmental disturbances. .
  • Development of Human disease Models using Micromini Pig
    keyword : Human disease model, Micromini Pig, Genome editing
    2014.04Generation and analysis of transgenic mice expressing transcription factors of neurotropic viruses Pseudorabies virus (PRV) infection in animals other than its natural host almost always gives rise to fatal diseases in the central nervous system as a result of infection of peripheral neurons and subsequent to the brain. To study the role of immediate-early protein (IE180) of PRV in neuropathogenicity, we have generated transgenic mouse lines expressing IE180 in a tetracycline-regulated system. The transgenic mice showed cerebellar symptoms such as ataxic gait, tremor and motor discoordination. In the transgenic mice, histopathology of the cerebellum was remarkable for a failure of layer formation and reduced cerebellum sizes. These findings suggest that IE180 affects the cascade of gene expression for development of the murine cerebellum. Borna disease virus infection causes a spectrum of behavioral deficits. To study the role of p24 of BDV in neuropathogenicity, we have generated transgenic mouse lines expressing p24 in astrocytes. The transgenic mice showed enhanced intermale aggressiveness and abnormalities in spatial learning. In the transgenic mice, brain-derived neurotrophic factor and synaptophysin were markedly decreased in hippocampus and cerebellum. These findings suggest that expression and accumulation of p24 in astrocytes creates neurodevelopmental disturbances..
Academic Activities
Reports
1. Etsuro Ono, Toshimitsu Uede, Implication of Soluble Forms of Cell Adhesion Molecules in Infectious Disease and Tumor: Insights from Transgenic Animal Models. , International Journal of Molecular Sciences, 10.3390/ijms19010239, 2018.01.
Papers
1. Yoshikazu Fujimoto, Yukiko Tomioka, Kinuyo Ozaki, Keiko Takeda, Haruka Suyama, Sayo Yamamoto, Hiroki Takakuwa, Masami Morimatsu, Toshimitsu Uede, Etsuro Ono, Comparison of the antiviral potential among soluble forms of herpes simplex virus type-2 glycoprotein D receptors, herpes virus entry mediator A, nectin-1 and nectin-2, in transgenic mice., Journal of General Virology, 98, 7, 1815-1822, 2017.07.
2. Yoshikazu Fujimoto, Yukiko Tomioka, Hiroki Takakuwa, Gen-ichiro Uechi, Toshiyo Yabuta, Kinuyo Ozaki, Haruka Suyama, Sayo Yamamoto, Masami Morimatsu, Le Q. May, Tetsu Yamashiro, Toshihiro Ito, Koichi Otsuki, Etsuro Ono, Cross-protective potential of anti-nucleoprotein human monoclonal antibodies against lethal influenza A virus infection., Journal of General Virology, 97, 9, 2104-2116, 2016.09.
3. Lopes-Ramos J C, Tomioka Y, Morimatsu M, Yamamoto S, Ozaki K, Ono E, Deldado-Garcia J M, Motor-coordination-dependent learning, more than others, is impaired in transgenic mice expressing pseudorabies virus immediate-early protein IE180, PloS ONE, 10.1371, 5, 8, e12123, 2010.08.
4. Tomioka Y, Miyazaki T, Taharaguchi S, Yoshino S, Morimatsu M, Uede T, Ono E and Watanabe M, Cerebellar pathology in transgenic mice expressing the pseudorabies virus immediate-early protein IE180, European Journal of Neuroscience , 27 (8) , 2115–2132 , 2008.04.
5. Ono E, Amagai K, Taharaguchi S, Tomioka Y, Yoshino S, Watanabe Y, Cherel P, Houdebine LM, Adam M, Eloit M, Inobe M, Uede T., Transgenic mice expressing a soluble form of porcine nectin-1/herpesvirus entry mediator C as a model for pseudorabies-resistant livestock., Proc Natl Acad Sci U S A, 101(46):16150-16155, 2004.11.
6. Ono E, Yoshino S, Amagai K, Taharaguchi S, Kimura C, Morimoto J, Inobe M, Uenishi T, Uede T., Enhanced resistance to herpes simplex virus type 1 infection in transgenic mice expressing a soluble form of herpesvirus entry mediator., Virology, 320(2):267-275, 2004.03.
7. Kamitani W, Ono E, Yoshino S, Kobayashi T, Taharaguchi S, Lee BJ, Yamashita M, Kobayashi T, Okamoto M, Taniyama H, Tomonaga K, Ikuta K., Glial expression of Borna disease virus phosphoprotein induces behavioral and neurological abnormalities in transgenic mice., Proc Natl Acad Sci U S A, 100(15):8969-8974, 2003.07.
8. Taharaguchi S, Kon Y, Yoshino S, Ono E., Impaired development of the cerebellum in transgenic mice expressing the immediate-early protein IE180 of pseudorabies virus., Virology, 307(2):243-254, 2003.03.
Membership in Academic Society
  • The Japanese Society for Virology, The Molecular Biology Society of Japan, The Japanese Society of Veterinary Science, Japanese Association for Laboratory Animal Sciences