Kyushu University Academic Staff Educational and Research Activities Database
Researcher information (To researchers) Need Help? How to update
Mako Nakamura Last modified date:2019.07.03

Associate Professor / Faculty of Agriculture, Center for Promotion of International Education and Research
Attached Promotive Center for International Education and Research of Agriculture
Faculty of Agriculture


Graduate School
Undergraduate School
Other Organization


E-Mail
Academic Degree
Ph.D.
Country of degree conferring institution (Overseas)
No
Field of Specialization
Developmental Biology, Cell Biology, Muscle Biology
ORCID(Open Researcher and Contributor ID)
0000-0002-6129-7560
Total Priod of education and research career in the foreign country
05years00months
Outline Activities
1. Research
My research focus is to understand mechanism of muscle stem cell development and Differentiation. There are several projects on going.
1) Muscle stem cell marker, Pax7 function on myocyte/adipocyte cell differentiation
2) Peroxisome specific marker Nudt7 involvement on muscle cell differentiation and muscle development
3) Nuclear membrane specific protein, emerin function on muscle cell differentiation.
4) Smooth muscle cell differentiation and development in chick gizzard.

2. Education
1) Problem Based Learning I, II (Graduate School of Bioresource and Bioenvironmental Sciences)
2) Advanced Animal and Marine Science (Graduate School of Bioresource and Bioenvironmental Sciences)
3) Genetics and Evolution (School of Interdisciplinary Science and Innovation)
4) Developmental Biology (School of Interdisciplinary Science and Innovation)

3. International Education
1). Management of short-term oversea programs in school of Agriculture
2). Management of short-term inbound programs in school of Agriculture and graduate school of Bioresource and Bioenvironmental Sciences.
Research
Research Interests
  • 1. Research
    My research focus is to understand mechanism of muscle stem cell development and Differentiation. There are several projects on going.
    1). Muscle stem cell marker, Pax7 function on myocyte/adipocyte cell differentiation
    2). Peroxisome specific marker Nudt7 involvement on muscle cell differentiation and muscle development
    3). Nuclear membrane specific protein, emerin function on muscle cell differentiation.
    keyword : Animal development, muscle, differentiation
    2002.04~2021.06.
Academic Activities
Papers
1. Nakamura M, Choe SK, Runko AP, Gardner PD, Sagerström CG., Nlz1/Znf703 acts as a repressor of transcription., BMC Dev Biol., 2008 Nov 12;8:108, 2008.11.
2. Nakamura M., Runko AP., and Sagerstrom CG, A novel subfamily of zinc finger genes involoved in embryonic development, J. Cell. Biochem., 2004.11.
Presentations
1. Mako Nakamura, Wakana Izumi, Wataru Mizunoya, and Ryuichi Tatsumi, Paired box 7 inhibits adipogenic differentiation -A possible role of Pax7 in non-myogenic cells-, Avian Model Systems 10, 2018.06, Myogenesis is precisely proceeded by myogenic regulatory factors. Myogenic stem cells are activated, proliferated and fused into a multinuclear myofiber. Pax7, paired box 7, one of the earliest markers during myogenesis. It has been reported that Pax7 regulates the muscle marker genes, Myf5 and MyoD toward differentiation. The possible roles of Pax7 in myogenic cells have been well researched. However, it has not yet been clarified if Pax7 itself is able to induce myogenic fate in non-myogenic lineage cells. We performed experiments using stably expressed Pax7 in 3T3-L1 preadipocytes to elucidate if Pax7 inhibits adipogenesis. These cells showed decreased expression of PDGFR, PPAR and Fabp4 and inhibited forming lipid droplets. However, they could not differentiate into myogenic cells. We think the Pax7 functions to pause cell differentiation and needs co-factors to induce into a specific cell type. To see the function of Pax7 in a cell type where possible myogenic co-factors may be available, we decided to use smooth muscle cells (SMCs). The SMCs culture model with E15 chick gizzard has been developed. The cells are cultured in the serum free medium supplemented with BSA and IGF-1. The cells are currently analyzed for the marker gene expression to find out if they keep the differentiation state..
Educational
Educational Activities
Class taught
1) Problem Based Learning I, II (Graduate School of Bioresource and Bioenvironmental Sciences)
2) English-Taught Lecture on Animal and Marine Bioresource Sciences
3) Genetics and Evolution (School of Interdisciplinary Science and Innovation)
4) Developmental Biology (School of Interdisciplinary Science and Innovation)

~2017
1) M05 Current Topics in Agriculture and Food Environment (Graduate School of Bioresource and Bioenvironmental Sciences) 2).Japanese Research Progress in Life Science (JTW program)
Other Educational Activities
  • 2010.08.