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Atsushi Tomokiyo Last modified date:2019.05.28

Lecturer / Oral rehabilitation
Oral Rehabilitation
Kyushu University Hospital


Graduate School
Administration Post
Other


E-Mail
Phone
092-642-6432
Fax
092-642-6366
Academic Degree
PhD
Country of degree conferring institution (Overseas)
No
Field of Specialization
Endodontic treatment
Total Priod of education and research career in the foreign country
02years01months
Outline Activities
The goal of endodontic treatment is to preserve teeth for a long time without pain. In addition, our research focuses on the regeneration of periodontal ligament. Periodontal ligament is a very important organ to fix teeth to alveolar bone. Therefore, the success of regeneration of periodontal ligament should prevent tooth extraction that is caused by periodontitis.
Research
Research Interests
  • Generation of a new method for periodontal regeneration using iPS cells and genomic drug discovery.
    keyword : iPS cells, genomic drug discovery, periodontal regeneration
    2018.04~2020.03.
  • Development of new periodontal regenerative therapy based on biomimetics using iPS cell-derived neural crest-like cells
    keyword : iPS cells, periodontal ligament, regeneration, biomimetics
    2017.04~2020.03.
  • A comparative analysis of MTA discoloration after the application of endodontic and restorative treatment-related solutions
    keyword : Mineral Trioxide Aggregate, Discoloration
    2015.07~2016.08.
  • The generation of neural crest like cells from human iPS cells and the application of iPSC-neural crest cells for tooth regeneratioin
    keyword : iPS cells, neural crest cells, tooth regeneration
    2014.10~2016.10.
  • Neural regenerative potential of periodontal ligament stem cells.
    keyword : stem cell, periodontal ligament, neuron, regeneraiton
    2009.04~2010.12.
  • The effect of AhR signaling on sollagen metabolism in human periodontal ligament cells
    keyword : periodontal ligament, AhR, dioxin, collagen metabolism
    2008.04~2009.03.
  • Effect of multipotent human periodontal ligament cell line on differentiation and migration of neurocyte
    keyword : periodontal ligament, neuron, stem cells, regeneration
    2008.07~2009.07Previously, some groups have reported the functional recovery of neurons by bone marrow derived mesenchymal stem cells. Therefore, we hypothesized that PDL stem cells also have neural regenerative potential, but there has been no report. Recently, we established a clonal human PDL cell line.These cells exhibited typical spindle-shaped fibroblastic morphologies, however they differentiated into osteoblast, chondrocyte, neurocyte, and adipocyte after respective induction. To investigate the neural regenerative potential of PDL stem cells, we aimed to analyze the effects of a multipotent PDL cell line on differentiation and migration of neurocyte. .
Current and Past Project
  • We research about the neural regenerative potential of the multipotent PDL cell line (cell line 1-17) to establish the new treatment for the neural regeneration.
Academic Activities
Books
1. Atsushi Tomokiyo, Naohisa Wada, Hidefumi Maeda, Contribution of Stem Cells to Dental Tissue Regeneration: Isolation, Function, and Application. , Bentham Science Publishers, Vol.2 p3-38, 2015.12.
Reports
1. Tomokiyo A, Yoshida S, Hamano S, Hasegawa D, Sugii H, Maeda H., Detection, Characterization, and Clinical Application of Mesenchymal Stem Cells in Periodontal Ligament Tissue., Stem Cells Int., 2018 Aug 26;2018:5450768. doi: 10.1155/2018/5450768. eCollection 2018., 2018.08.
2. Atsushi Tomokiyo, Naohisa Wada, Sayuri Hamano, Daigaku Hasegawa, Hideki Sugii, Shinichiro Yoshida, Hidefumi Maeda, Periodontal Ligament Stem Cells in Regenerative Dentistry for Periodontal Tissues, Journal of Stem Cell Research & Therapeutics, 2016.07, The ideal periodontal treatment is regenerating functional periodontal tissues destroyed by severe periodontitis. The tissue engineering triad includes stem cells, biological signals, and cell-seeded scaffold that are considered to be essential for tissue regeneration. Periodontal ligament (PDL) tissue contains a stem cell population, periodontal ligament stem cells (PDLSC), that is derived the neural crest tissue. PDLSC demonstrate high proliferative capacity and multipotency that make them a highly promising stem cell population for use in the regeneration of damaged periodontal tissues. Here, we review the current understanding of the features and functions of PDLSC..
Papers
1. Atsushi Tomokiyo, Shinichiro Yoshida, Sayuri Hamano, Daigaku Hasegawa, Hideki Sugii, Hidefumi Maeda, Detection, characterization, and clinical application of mesenchymal stem cells in periodontal ligament tissue, Stem Cells International, 10.1155/2018/5450768, 2018, 2018.01, Mesenchymal stem cells (MSCs) are a kind of somatic stem cells that exert a potential to differentiate into multiple cell types and undergo robust clonal self-renewal; therefore, they are considered as a highly promising stem cell population for tissue engineering. MSCs are identified in various adult organs including dental tissues. Periodontal ligament (PDL) is a highly specialized connective tissue that surrounds the tooth root. PDL also contains MSC population, and many researchers have isolated them and performed their detailed characterization. Here, we review the current understanding of the features and functions of MSC population in PDL tissues and discuss their possibility for the application of PDL regeneration..
2. Atsushi Tomokiyo, Kim Hynes, Jia Ng, Danijela Menicanin, Agnes Arthur, Esther Camp, Stan Gronthos, P. Mark Bartold, Generation of neural crest-like cells from human periodontal ligament cell-derived induced pluripotent stem cells., Journal of Cellular Physiology, 10.1002, 2016.05, Neural crest cells (NCC) are a transient multipotent stem cell population that migrates into its destination in the vertebrate embryo and gives rise to many different types of cells. NCC hold great promise for stem cell-based tissue engineering, however the inability to easily obtain large numbers of neural crest cells is a major factor limiting their use in studies of development and regenerative medicine. Induced pluripotent stem cells (iPSC) are emerging as a novel candidate that could provide an unlimited source of NCC because of their self-renewal capacity and pluripotency. In the present study, we examined the potential of neural crest tissue-derived periodontal ligament (PDL) iPSC to differentiate into neural crest-like cells (NCLC) relative to iPSC generated from a non-neural crest derived tissue, foreskin fibroblasts (FF). We detected high HNK1 expression during the differentiation of PDL and FF iPSC into NCLC as a marker for enriching for a population of cells with neural crest characteristics. Using magnetic-activated cell sorting, we isolated PDL iPSC- and FF iPSC-derived NCLC which highly expressed HNK1. The isolated NCLC also expressed the neural crest markers SLUG and SOX9. A high proportion of the HNK1-positive cell populations generated, expressed the MSC markers; CD73, CD90, CD105, CD146, and CD166, whilst very few cells expressed the pluripotency markers; TRA160, alkaline phosphatase, or the hematopoietic markers CD14, CD34, and CD45. The PDL and FF HNK1-positive populations gave rise to smooth muscle, neural, glial, osteoblastic and adipocytic like cells and exhibited higher expression of smooth muscle, neural, and glial cell-associated markers than the PDL and FF HNK1-negative populations. Interestingly the HNK1-positive cells derived from the PDL-iPSC exhibited a greater ability to differentiate into smooth muscle, neural, glial cells and adipocytes, than the HNK1-positive cells derived from the FF-iPSC. Our work suggests that HNK1-enriched NCLC from neural crest tissue-derived iPSC more closely resemble the phenotypic and functional hallmarks of NCC compared to the HNK1-low population and non-neural crest iPSC-derived NCLC..
3. Atsushi Tomokiyo, Hidefumi Maeda, Fujii Shinsuke, Monnouchi Satoshi, Naohisa Wada, Kono Kiyomi, Koori Katsuaki, naohide yamamoto, Yoko Teramatsu, Akifumi Akamine, Alternation of extracellular matrix remodeling and apoptosis by activation of the aryl hydrocarbon receptor pathway in human periodontal ligament cells, JOURNAL OF CELLULAR BIOCHEMISTRY, 10.1002/jcb.24186, 113, 10, 3093-3103, 2012.10.
4. Tomokiyo A, Maeda H, Fujii S, Monnouchi S, Wada N, Kono K, Koori K, Yamamoto N, Teramatsu Y, Akamine A. , A multipotent clonal human periodontal ligament cell line with neural crest cell phenotypes promotes neurocytic differentiation, migration, and survival.

, Journal of Cellular Physiology, 2011.07.
5. Tomokiyo A, Maeda H, Fujii S, Wada N, Shima K, Akamine A., Development of a multipotent clonal human periodontal ligament cell line., Differentiation. , 76(4):337-47, 2008.04.
Presentations
1. TOMOKIYO Atusushi, HAMANO Sayuri, HASEGAWA Daigaku, SUGII Hideki, YOSHIDA Shinichirou, MITARAI Hiromi, ARIMA Mai, NOZU Aoi, WADA Naohisa, MAEDA Hidefum, Discoloration of White Mineral Trioxide Aggregate Immersed in Various Solutions, 96th International Association of Dental Research, 2018.07.
Membership in Academic Society
  • Japanese society for regenerative medicine
  • Japanese Society of Gerodontology
Educational
Educational Activities
We give an education about clinical treatment for the students at 5 and 6 degree.
We give an advise about clinical treatment for the doctor-in-training.
We give an advise about dental research and clinical treatment for the PhD students.
Social
Professional and Outreach Activities
I conducted the dental students in Pusan university to our treatment area when they come to Kyushu university hospital.
I could not speak Korean, therefore I used English to explain the dental treatment methods and tools.
When the Brazilian researcher stayed in Japan, I gave advices for dental research adn explained the detail of dental treatment tools..