Kyushu University Academic Staff Educational and Research Activities Database
Researcher information (To researchers) Need Help? How to update
Kosaku Kurata Last modified date:2018.08.30

Associate Professor / Bioengineering
Department of Mechanical Engineering
Faculty of Engineering


Graduate School
Undergraduate School


E-Mail
Homepage
http://www.mech.kyushu-u.ac.jp/~hmt/HMT_lb_en.html
The heat and mass transfer laboratory in the Department of Mechanical Engineering under the direction of Prof. Takamatsu is dedicated particularly but not restricted to the studies in heat and mass transfer in biological systems. Research in this lab is broadly in the area of thermal engineering, bioengineering and biothermal engineering. .
Phone
092-802-3124
Fax
092-802-3127
Academic Degree
Doctor of Engineering
Country of degree conferring institution (Overseas)
No
Field of Specialization
Bioengineering, Biothermal Engineering
Total Priod of education and research career in the foreign country
02years05months
Outline Activities
Field of research and education;
Bioengineering, Cellular biomechanics, Biothermal engineering

Research subjects;
# Bone remodeling mechanism induced by microdamage
# Responses of bone-derived cells to mechanical stimulation
# Effect of mechanical vibration on bone mass and morphology
# Evaluation of thermal damage induced by bone cement polymerization
# Cell and tissue preservation at low temperature
# Visualization of freeze damage of biomacromolecules by using raman imaging
# Development of new tissue ablation therapy using irreversible electroporation
Research
Research Interests
  • Development of a series of open-source bio-hardware to inspire the talents with bio-innovative minds
    keyword : Open source, Biological experiment hardware, Active learning, Biotechnology innovation
    2017.07.
  • Inhibitory mechanism of cell division by spatiotemporally modulated electric field and its application

    keyword : Electric field, Mitosis, Tumor treatment, Tumor treating fields, Less invasive treatment
    2018.04.
  • Spectroscopic Quantification of ice-biomacromolecules interactions in frozen solutions using Raman imaging
    keyword : Raman imaging, Freeze damage, Biomacromolecules
    2011.04.
  • A study on nonthermal irreversible electroporation to ablate tumors
    keyword : Irreversible electroporation, Electropermeabilization, Electric field distribution, Temperature distribution, Tumor
    2011.04.
  • Effects of mechanical stimulation on the proliferation and differentiaton of mesenchymal stem cells
    keyword : Mesenchymal stem cells, Mechanical stimulation, Cell differentiation, Bone remodeling
    2012.04.
  • Mechanism of cell activation by thermal treatment and its application to tissue engineering
    keyword : Thermal treatment, Osteoblast, Bone formation, Tissue engineering
    2011.02~2012.03.
  • Study on heat conduction in bone and thermally-induced bone cell injury
    keyword : Thermal injury, Bone, Osteocyte, Heat conduction, Numerical analysis, Bone cement
    2010.04.
  • Initiating mechanism of bone remodeling by sensing fatigue microdamage
    keyword : Osteocyte, Osteoclast, Microdamage, Remodeling, Cellular network
    2009.04~2013.03.
  • Effect of mechanical vibration on bone mass and morphology
    keyword : Osteoporosis, Mechanical vibration, Bone remodeling
    2008.04.
Academic Activities
Reports
1. Guoliang Gu, Kosaku Kurata, Zhi Chen, Kalervo H. Väänänen, Osteocyte: a cellular basis for mechanotransduction in bone, Journal of Biomechanical Science and Engineering, 10.1299/jbse.2.150, Vol.2, No.4, pp.150-165, 2007.10, [URL].
Papers
1. Jan G. Hazenberg, Teuvo A. Hentunen, Terhi J. Heino, Kosaku Kurata, Thomas C. Lee, David Taylor, Microdamage detection and repair in bone
Fracture mechanics, histology, cell biology, Technology and Health Care, 10.3233/THC-2009-0536, 17, 1, 67-75, 2009.02, Bone is an elementary component in the human skeleton. It protects vital organs, regulates calcium levels and allows mobility. As a result of daily activities, bones are cyclically strained causing microdamage. This damage, in the form of numerous microcracks, can cause bones to fracture and therefore poses a threat to mechanical integrity. Bone is able to repair the microcracks through a process called remodelling which is tightly regulated by bone forming and resorbing cells. However, the manner by which microcracks are detected, and repair initiated, has not been elucidated until now. Here we show that microcrack accumulation causes damage to the network of cellular processes, resulting in the release of RANKL which stimulates the differentiation of cells specialising in repair..
2. Kosaku Kurata, Junpei Matsushita, Atsushi Furuno, Junichi Fujino, Hiroshi Takamatsu, Assessment of thermal damage in total knee arthroplasty using an osteocyte injury model, Journal of Orthopaedic Research, 10.1002/jor.23600, 35, 12, 2799-2807, 2017.12, Polymethylmethacrylate bone cement has been widely used for the anchorage of artificial implants in various orthopedic surgeries. Although it is one of the most successful biomaterials in use, excess heat generation intrinsically causes thermal damage to bone cells adjacent to the bone cement. To estimate a risk of thermal injury, a response of bone cells to cement polymerization must be elucidated because of the occurrence of thermal damage. Thermal damage is affected not only by maximal temperature but also by exposure time, temperature history, and cell type. This study aimed at quantifying the thermal tolerance of bone cells for the development of a thermal injury model, and applying this model for the estimation of thermal damage during cement polymerization in total knee arthroplasty. Osteocytes, osteoblasts, and fibroblasts were respectively subjected to steady supraphysiological temperatures ranging from 45 to 50°C. Survival curves of each cell and temperatures were used to formulate the Arrhenius model. A three-dimensional heat conduction analysis for total knee arthroplasty was conducted using the finite element model based on serial CT images of human knee. A maximal temperature rise of 50°C was observed at the interface between the 3-mm thick cement and the tissue immediately beneath the tibial tray of the prosthesis. The probability of thermal damage to the osteocyte, which was calculated using the Arrhenius model, was negligible at a distance of at least 1 mm away from the cement–bone interface..
3. Shuto Yoshimatsu, Masahiro Yoshida, Kosaku Kurata, Hiroshi Takamatsu, Development of contact irreversible electroporation using a comb-shaped miniature electrode, Journal of Thermal Science and Technology, 10.1299/jtst.2017jtst0023, 12, 2, 2017.08, Irreversible electroporation (IRE) has been studied as a less invasive method for tumor treatment. Since the mechanism of the treatment is based on the fatal perforation of the cell membrane caused by an external electric field, a tumor can be ablated non-thermally if an appropriate electric field is selected. However, an electric field more than a few kV/cm is required to accomplish ablation. In this study, we aim to examine the feasibility of a comb-shaped miniature electrode for reducing the required voltage for IRE. The reduction of the applied voltage while maintaining the potential difference was realized by narrowing the gap between the electrodes. A 150-μm-wide miniature electrode with a 100-μm gap between its teeth was fabricated using photolithography. In the experiment, the electrode was contacted onto a tissue phantom consisting of fibroblasts cultured in agarose gel three-dimensionally. After the application of electric pulses, cell ablation depth was examined using fluorescent staining. The miniature electrode successfully ablated the cells at the surface of the tissue phantom by the application of 90 electric pulses at 100 V. The maximum and average ablation depth were 72.7 μm and 61.0 ± 11 μm, respectively, which was approximately 40 % of that estimated by the numerical analysis. Our study showed that the contact-IRE using a miniature electrode in the order of sub-millimeter does ablate the superficial cells of targeted tissues upon the application of electric pulses of less than 100 V; however, further studies are required to maximize the ablation depth under the constraint of limited applied voltage..
4. Kosaku Kurata, Seiji Nomura, Hiroshi Takamatsu, Three-dimensional analysis of irreversible electroporation: Estimation of thermal and non-thermal damage, International Journal of Heat and Mass Transfer, 72, 66-74, 2014.05.
5. , [URL].
6. Kosaku Kurata, Ryo Ueno, Masahiro Matsushita, Takanobu Fukunaga, Hiroshi Takamatsu, Experimental and Analytical Studies on Contact Irreversible Electroporation for Superficial Tumor Treatment, Journal of Biomechanical Science and Engineering, https://doi.org/10.1299/jbse.8.306, 8, 4, 306-318, 2013.12, [URL].
7. Takashi Kono, Yasunori Ayukawa, Yasuko Moriyama, Kosaku Kurata, Hiroshi Takamatsu, Kiyoshi Koyano, The effect of low-magnitude, high-frequency vibration stimuli on the bone healing of rat incisor extraction socket, Journal of Biomechanical Engineering, 134, 9, 091001(6 pages), 2012.09.
8. Kosaku Kurata, Takashi Yoshii, Satoru Uchida, Takanobu Fukunaga, Hiroshi Takamatsu, Visualization of electroporation-induced temperature rise using temperature-sensitive ink, International Journal of Heat and Mass Transfer, 55, 23-24, 7207-7212, 2012.08.
9. Kosaku Kurata, Masahiro Matsushita, Takashi Yoshii, Takanobu Fukunaga, Hiroshi Takamatsu, Effect of Irreversible Electroporation on Three-Dimensional Cell Culture Model, Proceedings of the 34th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, 179-182, 2012.08.
10. Tomoki Nakashima, Mikihito Hayashi, Takanobu Fukunaga, Kosaku Kurata, Masatsugu Oh-hora, Jian Q Feng, Lynda F Bonewald, Tatsuhiko Kodama, Anton Wutz, Erwin F Wagner, Josef M Penninger, Hiroshi Takayanagi, Evidence for osteocyte regulation of bone homeostasis through RANKL expression, Nature Medicine, 17, 1231-1234, 2011.11.
11. Kosaku Kurata, Hiroshi Takamatsu, Effect of Hyperthermal Treatment on the Viability of Bone-Derived Cells, Journal of Biomechanical Science and Engineering, https://doi.org/10.1299/jbse.6.101, 6, 2, 101-113, 2011.04, [URL].
12. Hiroshi Takamatsu, Toshiyuki Tanaka, Yusaku Furuya, Satoru Uchida, Kosaku Kurata, Koji Takahashi, Preliminary Study of the Measurement of Thermal Conductivity of Fluids with a Micro-Beam MEMS Sensor, Proceedings of the 9th Asian Thermophysical Properties Conference, 2010.10.
13. Hideshi Miura, Kenta Okawachi, Hyun-Goo Kang, Fujio Tsumori, Kosaku Kurata, Nobuhiro Arimoto, Laser Forming of Ti-6Al-7Nb Alloy Powder Compacts for Medical Devices, Materials Science Forum, Vols. 654-656, pp. 2057-2060, 2010.06.
14. Hideshi Miura, Kenta Okawachi, Hyun-Goo Kang, Fujio Tsumori, Kosaku Kurata, Nobuhiro Arimoto, Laser Forming Technique For Medical Devices of Ti Alloy powders, Proceeding of the 13th International Conference on Metal Forming, pp.1308-1311, 2010.05.
15. S. Imai, T.J. Heino, A. Hienola, K. Kurata, K. B?ki, Y. Matsusue, H.K. V??n?nen, H. Rauvala, Osteocyte-derived HB-GAM (pleiotrophin) is associated with bone formation and mechanical loading, Bone, 10.1016/j.bone.2009.01.004, 44, 5, 785-794, Vol.44, No.5, pp.785-794, 2009.05.
16. T. Fukunaga, K. Kurata, J. Matsuda, H. Higaki, Effects of strain magnitude on mechanical responses of three-dimensional gel-embedded osteocytes studied with a novel 10-well elastic chamber, Journal of Biomechanical Science and Engineering, https://doi.org/10.1299/jbse.3.13, 3, 1, 13-24, Vol.3, No.1, pp.13-24, 2008.02, [URL].
17. K. KURATA, H. TANIGUCHI, T. FUKUNAGA, J. MATSUDA, H. HIGAKI, Development of a compact microbubble generator and its usefulness for three-dimensional osteoblastic cell culture, Journal of Biomechanical Science and Engineering, https://doi.org/10.1299/jbse.2.166, 2, 4, 166-177, 2007.10, [URL].
18. K. KURATA, T. FUKUNAGA, J. MATSUDA, H. HIGAKI, Role of mechanically damaged osteocytes in the initial phase of bone remodeling, International Journal of Fatigue, Vol.29, No.6, pp.1010-1018, 2007.06.
19. , [URL].
20. N. TSUKAMOTO, T. MAEDA, H. MIURA, S. JINGUSHI, A. HOSOKAWA, K. HARIMAYA, H. HIGAKI, K. KURATA, Y. IWAMOTO, Repetitive tensile stress to rat caudal vertebrae inducing cartilage formation in the spinal ligaments: a possible role of mechanical stress in the development of ossification of the spinal ligaments, Journal of Neurosurgery Spine, Vol.5, No.3, pp.234-242, 2006.09.
21. K. KURATA, T. J. HEINO, H. HIGAKI, H. K. V__N_NEN, Bone marrow cell differentiation induced by mechanically damaged osteocytes in 3D gel-embedded culture, Journal of Bone and Mineral Research, 10.1359/jbmr.060106, 21, 4, 616-625, Vol.21, No.4, pp.616-625, 2006.04.
22. K. KURATA, H. HIGAKI, H. MIURA, T. MAWATARI, T. MURAKAMI, Y. IWAMOTO, Influences of newly formed woven bone on tissue stresses in rat caudal vertebrae subjected to mechanical loading: A study based on morphological measurement using a micro-CT and computational stress analysis, JSME International Journal, Series C, 10.1299/jsmec.45.558, 45, 2, 558-566, Vol.45, No.2, pp.558-566, 2002.06, [URL].
23. K. KURATA, T. UEMURA, A. NEMOTO, T. TATEISHI, T. MURAKAMI, H. HIGAKI, H. MIURA, Y. IWAMOTO, Mechanical strain effect on bone resorbing activity and mRNA expressions of marker enzymes in isolated osteoclast culture, Journal of Bone and Mineral Research, 10.1359/jbmr.2001.16.4.722, 16, 4, 722-730, Vol.16, No.4, pp.722-730, 2001.04.
24. K. KURATA, H. HIGAKI, H. MIURA, T. MURAKAMI, Y. IWAMOTO, Alteration of mechanical properties of remodeling bone adapted to mechanical stimuli, JSME International Journal, Series C, https://doi.org/10.1299/jsmec.43.822, 43, 4, 822-829, Vol.43, No.4, pp.822-829, 2000.12, [URL].
25. , [URL].
26. K. KURATA, H. HIGAKI, H. MIURA, T. MAWATARI, T. MURAKAMI, Y. IWAMOTO, The morphological measurements with a micro CT and the stress analyses of the adaptive remodeling by applied mechanical stimuli in rat caudal vertebrae, JSME International Journal, Series C, 10.1299/jsmec.42.492, 42, 3, 492-500, Vol.42, No.3, pp.492-500, 1999.09, [URL].
Presentations
1. Kosaku Kurata, Development of a rotary bending device for 3D cell culture, 2nd Bone and Biomaterials Workshop, 2016.08.
Membership in Academic Society
  • Heat Transfer Society of Japan
  • International Bone and Mineral Society
  • Japanese Society for Bone Morphometry
  • The Japanese Society for Bone and Mineral Research
  • The Japan Society of Mechanical Engineers