Kyushu University Academic Staff Educational and Research Activities Database
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Haruyoshi Yamaza Last modified date:2019.05.25

Associate Professor / Division of Oral Health, Growth and Development
Department of Dental Science
Faculty of Dental Science


Graduate School
Undergraduate School
Other Organization


E-Mail
Phone
092-642-6402
Fax
092-642-6468
Academic Degree
Ph.D
Country of degree conferring institution (Overseas)
No
Field of Specialization
Pediatric Dentistry, Special Needs Dentistry
ORCID(Open Researcher and Contributor ID)
0000-0003-1138-3943
Total Priod of education and research career in the foreign country
02years04months
Research
Research Interests
  • Study of stem cells from human exfoliated deciduous teeth
    keyword : mesenchymal stem cell, primary tooth
    2009.04.
  • Elucidation of extened lifespan by caloric restriction
    keyword : caloric restriction longevity
    2001.04.
  • Molecular analysis of odontogenesis
    keyword : odontogenesis
    1997.04.
Academic Activities
Papers
1. Haruyoshi Yamaza, Soichiro Sonoda, Kazuaki Nonaka, Toshio Kukita, Takayoshi Yamaza, Pamidronate decreases bilirubin-impaired cell death and improves dentinogenic dysfunction of stem cells from human deciduous teeth, Stem Cell Research and Therapy, 10.1186/s13287-018-1042-7, 9, 1, 2018.11, BACKGROUND: Hyperbilirubinemia that occurs in pediatric liver diseases such as biliary atresia can result in the development of not only jaundice in the brain, eyes, and skin, but also tooth abnormalities including green pigmentation and dentin hypoplasia in the developing teeth. However, hyperbilirubinemia-induced tooth impairments remain after liver transplantation. No effective dental management to prevent hyperbilirubinemia-induced tooth impairments has been established. METHODS: In this study, we focused on pamidronate, which is used to treat pediatric osteopenia, and investigated its effects on hyperbilirubinemia-induced tooth impairments. We cultured stem cells from human exfoliated deciduous teeth (SHED) under high and low concentrations of unconjugated bilirubin in the presence or absence of pamidronate. We then analyzed the effects of pamidronate on the cell death, associated signal pathways, and dentinogenic function in SHED. RESULTS: We demonstrated that a high concentration of unconjugated bilirubin induced cell death in SHED via the mitochondrial pathway, and this was associated with the suppression of AKT and extracellular signal-related kinase 1 and 2 (ERK1/2) signal pathways and activation of the nuclear factor kappa B (NF-κB) signal pathway. The high concentration of unconjugated bilirubin impaired the in vitro and in vivo dentinogenic capacity of SHED, but not the low concentration. We then demonstrated that pamidronate decreased the bilirubin-induced cell death in SHED via the altered AKT, ERK1/2, and NF-κB signal pathways and recovered the bilirubin-impaired dentinogenic function of SHED. CONCLUSIONS: Our findings suggest that pamidronate may prevent tooth abnormalities in pediatric patients with hyperbilirubinemia..
2. Haruyoshi Yamaza, E. Tomoda, S. Sonoda, K. Nonaka, Toshio Kukita, Takayoshi Yamaza, Bilirubin reversibly affects cell death and odontogenic capacity in stem cells from human exfoliated deciduous teeth, Oral Diseases, 10.1111/odi.12827, 24, 5, 809-819, 2018.01, Objective: Hyperbilirubinemia in patients with biliary atresia causes deciduous tooth injuries such as green pigmentation and dentin hypoplasia. In patients with biliary atresia who received liver transplantation, tooth structure appears to be recovered radiographically. Nevertheless, little is known about cellular mechanisms underlying bilirubin-induced damage and suppression of deciduous tooth formation. In this study, we examined the effects of bilirubin in stem cells from human exfoliated deciduous teeth (SHED) in vitro. Materials and Methods: SHED were cultured under exposure to excess of bilirubin and then interruption of bilirubin stimulation. Results: Bilirubin induced cell death and inhibited the odontogenic capacity of SHED by suppressing AKT and extracellular signal-regulated kinase 1 and 2 (ERK1/2) pathways and enhancing nuclear factor kappa B p65 (NF-κB p65) pathway. The interruption of bilirubin stimulation reduced cell death and recovered the inhibited odontogenic capacity of bilirubin-damaged SHED. The bilirubin interruption also normalized the impaired AKT, ERK1/2, and NF-κB p65 signaling pathways. Conclusion: These findings suggest that tooth hypodontia in patients with hyperbilirubinemia might be due to bilirubin-induced cell death and dentinogenic dysfunction of odontogenic stem cells via AKT, ERK1/2, and NF-κB pathways and also suggested that bilirubin-induced impairments in odontogenic stem cells were reversible when bilirubin stimulation is interrupted..
3. Jingxian Fang, Haruyoshi Yamaza, Takeshi Uchiumi, Yoshihiro Hoshino, Keiji Masuda, Yuta Hirofuji, Frank A.D.T.G. Wagener, Dongchon Kang, Kazuaki Nonaka, Dihydroorotate dehydrogenase depletion hampers mitochondrial function and osteogenic differentiation in osteoblasts, European Journal of Oral Sciences, 10.1111/eos.12270, 124, 3, 241-245, 2016.06, Mutation of the dihydroorotate dehydrogenase (DHODH) gene is responsible for Miller syndrome, which is characterized by craniofacial malformations with limb abnormalities. We previously demonstrated that DHODH was involved in forming a mitochondrial supercomplex and that mutated DHODH led to protein instability, loss of enzyme activity, and increased levels of reactive oxygen species in HeLa cells. To explore the etiology of Miller syndrome in more detail, we investigated the effects of DHODH inhibition in the cells involved in skeletal structure. Dihydroorotate dehydrogenase in MC3T3-E1 cells derived from mouse calvaria osteoblast precursor cells was knocked down by specific small interfering RNAs (siRNAs), and cell proliferation, ATP production, and expression of bone-related genes were investigated in these cells. After depletion of DHODH using specific siRNAs, inhibition of cell proliferation and cell cycle arrest occurred in MC3T3-E1 cells. In addition, ATP production was reduced in whole cells, especially in mitochondria. Furthermore, the levels of runt-related transcription factor 2 (Runx2) and osteocalcin (Ocn) mRNAs were lower in DHODH siRNA-treated cells compared with controls. These data suggest that depletion of DHODH affects the differentiation and maturation of osteoblasts. This study shows that mitochondrial dysfunction by DHODH depletion in osteoblasts can be directly linked to the abnormal bone formation in Miller syndrome..
4. Ryotaro Kamohara, Haruyoshi Yamaza, Tomoshi Tsuchiya, Toshimitsu Komatsu, Seongjoon Park, Hiroko Hayashi, Takuya Chiba, Ryoichi Mori, Shuichi Otabe, Kentaro Yamada, Takeshi Nagayasu, Isao Shimokawa, Overexpression of the adiponectin gene mimics the metabolic and stress resistance effects of calorie restriction, but not the anti-tumor effect, EXPERIMENTAL GERONTOLOGY, 10.1016/j.exger.2015.02.011, 64, 46-54, 2015.04.
5. Yamaza H, Komatsu T, Wakita S, Kijogi C, Park S, Hayashi H, Chiba T, Mori R, Furuyama T, Mori N, Shimokawa I., FoxO1 is involved in the antineoplastic effect of calorie restriction, Aging Cell, 2010.03.
6. Zhan M.*, Yamaza H.*, Sun Y., Sinclair J., Li H. and Zou S. (*These authors contributed equally to this work) , Temporal and Spatial Transcriptional Profiles of Aging in Drosophila melanogaster, Genome Res., 17(8): 1236-1243, 2007.08.
7. Yamaza H., Komatsu T., To K., Toyama H., Chiba T., Higami Y. and Shimokawa I. , Involvement of insulin-like growth factor-1 in the effect of calorie restriction; regulation of plasma adiponectin and leptin. , J. Gerontol. A Biol. Sci. Med. Sci., 62(1): 27-33, 2007.01.
8. Yamaza H., Komatsu T., Chiba T., Toyama H., To K., Higami Y. and Shimokawa I. , A transgenic dwarf rat model as a tool for the study of calorie restriction and aging., Exp. Gerontol., 39 (2): 269-272, 2004.02.
9. Yamaza H., Matsuo K., Kiyoshima T., Shigemura N., Kobayashi I., Wada H., Akamime A. and Sakai H., Detection of differentially expressed genes in the early developmental stage of the mouse mandible. , Int. J. Dev. Biol. , 45, 4, 675-680, 45(4): 675-680, 2001.06.
10. Yamaza H., Matsuo K., Kobayashi I., Wada H., Kiyoshima T., Akhtar M., Ishibashi Y., Sakai T., Akamine A. and Sakai H. , Expression of set-alpha during morphogenesis of mouse lower first molar. , Histochem. J. , 10.1023/A:1014491111628, 33, 8, 437-441, 33(8): 437-441, 2001.05.
Presentations
1. @Haruyoshi Yamaza, Bilirubin reversibly affects cell death and odontogenic capacity of SHED, ASCB|EMBO 2018 meeting, 2018.12.
2. Bilirubin reversibly affects cell death and odontogenic capacity in stem cells from human exfoliated deciduous teeth.
3. Haruyoshi Yamaza, Dihydroorotate dehydrogenase depletion hampersmitochondrial function and osteogenic differentiation in osteoblasts, 頭脳循環Kick Off Symposium, 2015.02.
4. Haruyoshi Yamaza, Inhibition of mitochondrial function by depletion of Dihydroorotate dehydrogenase depletion in osteoblast, The 25th Fukuoka International Symposium On Pediatiric/Maternal-Child Health Research, 2014.08.
5. Haruyoshi Yamaza, Kazuaki Nonaka, Dihydroorotate dehydrogenase depletion inhibits mitochondrial function in osteoblasts, Gordon Research Conferences: Craniofacial Morphogenesis & Tissue Regeneration, 2014.03.
6. Haruyoshi Yamaza, SHED in regenerative dental medicine, USJI Week Event5: Contribution of US-Japan exchange of researchers in development of the molecular basis of dental and maxillofacial regenerative medicine leading collaboration among South-East Asian countries and US-Japan, 2013.09.
7. Haruyoshi Yamaza, Caloric Restriction in Health, Kyudai Oral Bioscience 2013 -7th International Symposium-, 2013.03.
8. Haruyoshi Yamaza, Stem cell researches in regenerative dentistry and the future., USJI Week Event4: Craniofacial Translational Research Based on Molecular Craniofacial Developmental Research, 2012.09.
Membership in Academic Society
  • American Aging Association
  • Japanese Socoety of Pathology
  • Japan Society for Biomedical Gerontology
  • Japanese Association for Oral Biology
  • Japanese Society for Disability and Oral Health
  • Japanese Society of Pediatric Dentistry
Awards
  • Award for good poster presentation
  • Travel Support for attending the American Aging Association 35th Annual Meeting and poster presentation.
Educational
Educational Activities
Pre-clinical training for Pediatric dentistry
Clinical training for Pediatric dentistry
Lecture of Pediatric dentistry
Lecture for Special needs dentistry
Other Educational Activities
  • 2019.03, The external reviewer for OSCE at Osaka Dental University on March, 2019.
  • 2018.09, The internal reviewer for OSCE at Kyushu University school of dentistry on September, 2018.
  • 2018.08, The external reviewer for OSCE at Tokyo Medicak and Dental University on August, 2018.
  • 2017.09, The internal reviewer for OSCE at Kyushu University school of dentistry on September, 2017.
  • 2017.07.
  • 2015.01.
  • 2015.09, The internal reviewer for OSCE at Kyushu University school of dentistry on September, 2015.
  • 2014.09, The internal reviewer for OSCE at Kyushu University school of dentistry on September, 2014.
  • 2014.09, The internal reviewer for OSCE at Kyushu University school of dentistry on September, 2014.
  • 2013.03.
  • 2013.09, The internal reviewer for OSCE at Kyushu University school of dentistry on September, 2013.
  • 2013.11.
  • 2012.09, The internal reviewer for OSCE at Kyushu University school of dentistry on September, 2012.
  • 2011.09, The internal reviewer for OSCE at Kyushu University school of dentistry on September ,2011.
  • 2010.01.
  • 2010.01.
  • 2010.09, The internal reviewer for OSCE at Kyushu University school of dentistry on September ,2010.
  • 2009.09, The internal reviewer for OSCE at Kyushu University school of dentistry on September ,2009.
  • 2009.10.
Social
Professional and Outreach Activities
Oral health examination at Showa-Gakuenn in Fukutsu City, Japan
Oral health examination at Eucaly-Gakuenn, Institute for the handicapped, in Kurume City, Japan
Oral health examination at Center for the handicapped in Fukuoka City, Japan
Oral health examination at public health center of Hakata-ku and Minami-ku in Fukuka City, Japan
.