Kyushu University Academic Staff Educational and Research Activities Database
List of Papers
Hiroyuki Sasaki Last modified date:2019.03.26

Professor / Department of Molecular and Structural Biology / Medical Institute of Bioregulation


Papers
1. Ishiuchi, T., Ohishi, H., Sato, T., Kamimura, S., Yorino, M., Abe, S., Suzuki, A., Wakayama, T., Suyama, M. & Sasaki, H., Zfp281 shapes the transcriptome of Trophoblast stem cells and is essential for placental development. , Cell Rep. (in Press), 2019.03.
2. Au Yueng, W.K., Brind’Amour, J., Hatano, Y., Yamagata, K., Feil, R., Lorincz, M.C., Tachibana, M., Shinkai, Y. & Sasaki, H., Histone H3K9 methyltransferase G9a in oocytes is essential for preimplantation development but dispensable for CG methylation protection.
, Cell Rep. (in press), 2019.03.
3. Kamae, C., Imai, K., Kato, T., Okano, T., Honma, K., Nakagawa, N., Yeh, T.-W., Noguchi, E., Ohara, A., Shigemura, T., Takahashi, H., Takakura, S., Hayashi, M., Honma, A., Watanabe, S., Shigemori, T., Ohara, O., Sasaki, H., Kubota, T., Morio, T., Kanegae, H. & Nonoyama, S., Clinical and immunological characterization of ICF syndrome in Japan. , J. Clin. Immunol. (in press)., 2019.03.
4. Kubo, N., Harada, T., Shiraishi, Y., Nosaki, K., Nakagaki, N., Takeshita, M., Ouchi, H., Iwama, E., Tanaka, K., Okamoto, I., Sasaki, H. & Nakanishi, Y. (in press), Identification of Genomic Alterations Acquired During Treatment with EGFR-TKIs in Non-small Cell Lung Cancer., Anticancer Res., 2018.12.
5. Kamae, C., Imai, K., Kato, T., Okano, T., Honma, K., Nakagawa, N., Yeh, T.-W., Noguchi, E., Ohara, A., Shigemura, T., Takahashi, H., Takakura, S., Hayashi, M., Honma, A., Watanabe, S., Shigemori, T., Ohara, O., Sasaki, H., Kubota, T., Morio, T., Kanegae, H. & Nonoyama, S., Clinical and immunological characterization of ICF syndrome in Japan., J. Clin. Immunol. (in press), 2018.10.
6. Unoki, M., Funabiki, H., Velasco, G., Francastel, C. & Sasaki, H. , CDCA7 and HELLS mutations undermine non-homologous end joining in centromeric instability syndrome., J. Clin. Invest. (in press), 2018.10.
7. Yamashiro, C., Sasaki, K., Yabuta, Y., Kojima, Y., Nakamura, T., Okamoto, I., Yokobayashi, S., Murase, Y., Ishikura, I., Shirane, K., Sasaki, H., Yamamoto, T. & Saitou, M., Generation of human oogonia from induced pluripotent stem cells in vitro., Science (in press), 2018.09.
8. Sakakibara, Y., Nagao, K., Blewitt, M., Sasaki, H., Obuse, C. & Sado, T., Role of SmcHD1 in establishment of epigenetic states required for the maintenance of the X-inactivated state in mice., Development (in press), 2018.08.
9. Matsuda, S., Yasukawa, T., Sakaguchi, Y., Ichiyanagi, K., Unoki, M., Gotoh, K., Fukuda, K., Sasaki, H., Suzuki, T. & Kang, D., Accurate estimation of 5-methylcytosine in mammalian mitochondrial DNA., Sci. Rep., 2018.03.
10. Shimosuga, K., Fukuda, K., Sasaki, H. & Ichiyanagi, K., Locus-specific hypomethylation of the mouse IAP retrotransposon is associated with transcription factor-binding sites., Mobile DNA, 8, 20, 2017.12.
11. Suzuki, A., Kawano, S., Mituyama, T., Suyama, M., Kanai, Y., Shirahige, K., Sasaki, H., Tokunaga, K., Tsuchihara, K., Sugano, S., Nakai, K. & Suzuki, Y., DBTSS/DBKERO for integrated analysis of transcriptional regulation., Nucleic Acids Res., 46, D229-D238, 2017.11.
12. Okae, H., Toh, H., Sato, T., Hiura, H., Takahashi, S., Shirane, K., Kabayama, Y., Suyama, M., Sasaki, H. & Arima, T., Derivation of human trophoblast stem cells, Cell Stem Cell, 22, 50-63, 2017.11, 国際ヒトエピゲノムコンソーシアム(International Human Epigenome Consortium, IHEC)に参画して決定した各種ヒト胎盤細胞の遺伝子発現データを利用し、世界で初めてヒト胎盤幹細胞を培養する技術を確立することに成功した。この細胞や技術を用いることで、ヒトの胎盤の形成機構や特性を詳細に解明できるほか、妊娠高血圧症や胞状奇胎などの病因解明と治療法の確立、各種薬剤の胎盤通過性のスクリーニング、人工胎盤の作出などが容易になることが期待される。.
13. Maenohara, S., Unoki, M., Toh, H., Ohishi, H., Sharif, J., Koseki, H. & Sasaki, H., Role of UHRF1 in de novo DNA methylation in oocytes and maintenance methylation in preimplantation embryos, PLoS Genet., 13, e1007042, 2017.10.
14. Chigi, Y., Sasaki, H. & Sado, T., The 5’ region of Xist RNA has the potential to associate with chromatin through the A-repeat., RNA, 23, 1894-1901, 2017.12.
15. Inoue, K., Ichiyanagi, K., Fukuda, K., Glinka, M. & Sasaki, H., Switching of dominant retrotransposon silencing strategies from posttranscriptional to transcriptional mechanisms during male germ-cell development in mice, PLoS Genet., 13, e1006926, 2017.07.
16. Sakata, Y., Nagao, K., Hoki, Y., Sasaki, H., Obuse, C. & Sado, T., Defects in dosage compensation impact global gene regulation in the mouse trophoblast., Development, 144, 2784-2797, 2017.08.
17. Fukuda, K., Inoguchi, Y., Ichiyanagi, K., Ichiyanagi, T., Go, Y., Nagano, M., Yanagawa, Y., Takaesu, N., Ohkawa, Y., Imai, H. & Sasaki, H., Evolution of the sperm methylome of primates is associated with retrotransposon insertions and genome instability., Hum. Mol. Genet., 26, 3508-3519, 2017.09.
18. Ohta, H., Kurimoto, K., Okamoto, I., Nakamura, T., Yabuta, Y., Miyauchi, H., Yamamoto, T., Okuno, Y., Hagiwara, M., Shirane, K., Sasaki, H. & Saitou, M., In vitro expansion of mouse primordial germ cell-like cells recapitulates an epigenetic blank slate, EMBO J., 36, 1888-1907, 2017.07.
19. Hidaka, N., Iwama, E., Kubo, N., Harada, T., Miyawaki, K., Tanaka, K., Okamoto, I., Baba, E., Akashi, K., Sasaki, H. & Nakanishi, Y., Most T790M mutations are present on the same EGFR allele as activating mutations in patients with non-small cell lung cancer, Lung Cancer, 108, 75-82, 2017.02.
20. Kabayama, Y., Toh, H., Katanaya, A., Sakurai, T., Chuma, S., Kuramochi-Miyagawa, S., Saga, Y., Nakano, T. & Sasaki, H., Roles of MIWI, MILI, and PLD6 in small RNA regulation in mouse growing oocytes, Nucl. Acids Res., 45, 5387-5398, 2017.05, 配偶子において転移性遺伝因子による挿入突然変異を防ぐ小分子RNAに着目し、変異マウスを用いて卵子におけるその合成経路を初めて解明した。また、卵子形成過程では精子形成過程とは異なり、特定の少数の転移性遺伝因子だけがこのRNAで制御されるなど、ゲノムの防御システムに性的二形があることを明らかにした.
21. Kobayashi, M., Kato, H., Hada, H., Itoh-Nakadai, A., Fujiwara, T., Muto, A., Inoguchi, Y., Ichiyanagi, K., Houjou, W., Tomosugi, N., Sasaki, H., Harigae, H. & Igarashi, K., Iron-heme-Bach1 axis is involved in erythroblast adaptation to iron deficiency., Haematologica, 102, 454-465, 2017.03.
22. Toh, H., Shirane, K., Miura, F., Kubo, N., Ichiyanagi, K., Hayashi, K., Saitou, M., Suyama, M., Ito, T. & Sasaki, H., Software updates in the Illumina HiSeq platform affect whole-genome bisulfite sequencing., BMC Genomics, 18, 31, 2017.01.
23. Ishikura, Y., Yabuta, Y., Ohta, H., Hayashi, K., Nakamura, T., Okamoto, I., Yamamoto, T., Kurimoto, K., Shirane, K., Sasaki, H. & Saitou, M., In vitro derivation and propagation of spermatogonial stem cell activity from mouse pluripotent stem cells., Cell Rep., 17, 2789-2804, 2016.12.
24. Hamada, H., Okae, H., Toh, H., Chiba, H., Hiura, H., Shirane, K., Sato, T., Suyama, M., Yaegashi, N., Sasaki, H. & Arima, T., Allele-specific methylome and transcriptome analysis reveals widespread imprinting in the human placenta., Am. J. Hum. Genet., 99, 1045-1058, 2016.11.
25. Shirane, K., Kurimoto, K., Yabuta, Y., Yamaji, M., Satoh, J., Ito, S., Watanabe, A., Hayashi K., Saitou, M. & Sasaki, H., Global landscape and regulatory principles of DNA methylation reprogramming for germ cell specification by mouse pluripotent stem cells., Dev. Cell, 39, 87-103, 2016.10, 配偶子の形成にはDNAメチル化などのエピゲノムの制御が重要であり、その制御機構の解明は不妊や流産などの克服に資する。我々は高精度で網羅的なDNAメチル化解析法を用いて、京大・斎藤通紀らとの共同研究により、培養下の胚性幹細胞から誘導された始原生殖細胞様細胞のメチル化状態を解析し、そのメチル化状態は基本的に生体内のそれに酷似すること、転写制御因子Prdm14が始原生殖細胞様細胞におけるメチル化パターンの形成に必須であることを明らかにした。.
26. Amouroux, R., Nashun, B., Shirane, K., Nakagawa, S., Hill, P.W., D’Souza, Z., Nakayama, M., Matsuda, M., Turp, A., Ndjetehe, E., Encheva, V., Kudo, N.R., Koseki, H., Sasaki, H. & Hajkova, P., De novo DNA methylation drives 5hmC accumulation in mouse zygotes., Nat. Cell Biol., 18, 225-233, 2016.02.
27. Amakawa, Y., Sakata, Y., Hoki, Y., Arata, S., Shioda, S., Fukagawa, T., Sasaki, H. & Sado, T., A new Xist allele driven by a constitutively active promoter is dominated by Xist locus environment and exhibits the parent-of-origin effects, Development, 142, 4299-4308, 2015.12.
28. Kubo, N., Toh, H., Shirane, K., Shirakawa, T., Kobayashi, H., Sato, T., Sone, H., Sato, Y, Tomizawa, S., Tsurusaki, Y., Shibata, H., Saitsu, H., Suzuki, Y., Matsumoto, N, Suyama, M., Kono, T., Ohbo, K. & Sasaki, H., DNA methylation and gene expression dynamics during spermatogonial stem cell differentiation in the early postnatal mouse testis., BMC Genomics, 16, 1, 624, 2015.08.
29. Thijssen, P.E., Ito, Y., Grillo, G., Wang, J., Velasco, G., Nitta, H., Unoki, M., Yoshihara, M., Suyama, M., Sun, Y., Lemmers, R.J.L.F., de Greef, J.C., Gennery, A., Picco, P., Kloeckener-Gruissem, B., Güngör, T., Reisli, I., Picard, C., Kebaili, K., Roquelaure, B., Iwai, T., Kondo, I., Kubota, T., van Ostaijen-Ten Dam, M.M., van Tol, M.J.D., Weemaes, C., Francastel, C., van der Maarel, S.M. & Sasaki, H., Mutations in CDCA7 and HELLS cause immunodeficiency, centromeric instability and facial anomalies syndrome., Nat. Commun., 28, 6, 7870, 2015.06, 常染色体劣性遺伝病であるICF症候群の原因遺伝子としてCDCA7とHELLSの2つを発見した。ICF症候群は先天性の免疫不全症で、染色体の動原体領域のDNA低メチル化が特徴的である。今回の研究成果はICF症候群の確定診断と早期治療に役立つばかりでなく、将来的に遺伝子治療の開発へと繋がるものである。また、発見された2つの遺伝子がどのように動原体のDNAメチル化に関わるのかを解明することで、染色体の安定性や免疫細胞の分化の機構が明らかになる。.
30. Ichiyanagi, T., Ichiyanagi, K., Ogawa, A., Kuramochi-Miyagawa, S., Nakano, T., Sasaki, H. & Udono, H., HSP90 alpha plays an important role in piRNA biogenesis and retrotransposon repression in mouse, Nucleic Acids Res., 42, 11903-11911, 2014.10.
31. Liu, S., Brind’Amour, J., Karimi, M.M., Shirane, K., Bogutz, A., Lefebvre, L., Sasaki, H., Shinkai, Y. & Lorincz, M.C., Setdb1 is required for germ line development and silencing of H3K9me3 marked endogenous retroviruses in primordial germ cells., Genes Dev., 8, 2041-2055, 2014.09.
32. Velasco, G., Walton, L. E., Sterlin, D., Hedouin, S., Nitta, H., Ito, Y., Fouyssac F., Megarbane, A., Sasaki, H., Picard, C. & Francastel, C., Germline genes hypomethylation and expression define a molecular signature in peripheral blood of ICF patients: implications for diagnosis and etiology., Orphanet J. Rare Dis., 9, 56, 2014.04.
33. Shiura, H., Okamoto, A., Sasaki, H. & Abe, K., Whole-mount MeFISH: a novel technique for simultaneous visualization of specific DNA methylation and protein/RNA expression., PloS One, 9, e95750, 2014.04.
34. Liao, H.-F., Chen, W.S.C., Chen, Y.-H., Kao, T.-H., Tseng, Y.-T., Lee, C.-Y., Chiu, Y.-C., Lee, P.-L., Lin, Q.-J., Ching, Y.-H., Hata, K, Cheng, W.T.K., Tsai, M.-H., Sasaki, H., Ho, H.-N., Wu, S.-C., Huang, Y.-H., Yen, P. & Lin, S.-P., DNMT3L promotes quiescence in postnatal spermatogonial progenitor cells., Development, 141, 2402-2413, 2014.05.
35. Yokomizo, H., Inoguchi, T., Sonoda, N., Sakaki, Y., Maeda, Y., Inoue, T., Hirata, E., Takei, R., Ikeda, N., Fujii, M., Fukuda, K., Sasaki, H. & Takayanagi, R., Maternal high fat diet induces insulin resistance and deterioration of pancreatic β cell function in adult offspring with gender differences in mice., Am. J. Physiol. Endocrinol. Metab., 306, E1163-E1175, 2014.04.
36. Okae, H., Matoba, S., Nagashima, T, Mizutani, E, Inoue, K., Ogonuki, N., Chiba, H., Funayama, R., Tanaka, S., Yaegashi, N., Nakayama, K., Sasaki, H., Ogura, A. & Arima, T., RNA sequencing-based identification of aberrant imprinting in cloned mice., Hum. Mol. Genet., 23, 992-1001, 2014.02.
37. Li, Y., Miyanari, Y., Shirane, K., Nitta, H., Kubota, T., Ohashi, H., Okamoto, A. & Sasaki, H., Sequence-specific microscopic visualization of DNA methylation status at satellite repeats in individual cell nuclei and chromosomes., Nucl. Acids Res., 41, e186, 2013.10.
38. Nitta, H., Unoki, M., Ichiyanagi, K., Kosho, T., Takahashi, H., Velasco, G., Francastel, C., Picard, C., Kubota, T. & Sasaki, H., Three novel ZBTB24 mutations identified in Japanese and Cape Verdean type 2 ICF syndrome patients., J. Hum. Genet., 58, 455-460, 2013.06.
39. Fukuda, K., Ichiyanagi, K., Yamada, Y., Go, Y., Udono, T., Wada, S., Maeda, T., Soejima, H., Saitou, N., Ito, T. & Sasaki, H., Regional DNA methylation differences between humans and chimpanzees are associated with genetic changes, transcriptional divergence and disease genes., J. Hum. Genet., 58, 446-454, 2013.06.
40. Shirane, K., Toh, H., Kobayashi, H., Miura, F., Chiba, H., Ito, T., Kono, T. & Sasaki, H., Mouse oocyte methylomes at base resolution reveal genome-wide accumulation of non-CpG methylation and role of DNA methyltransferases. PLoS Genet. 9, e1003439 (2013)., PLoS Genet., 9, e1003439, 2013.04.
41. Unoki, M., Masuda, A., Dohmae, N., Arita, K., Yoshimatsu, M., Iwai, Y., Fukui, Y., Ueda, K., Hamamoto, R., Shirakawa, M., Sasaki, H. & Nakamura, Y., Lysyl 5-Hydroxylation, a Novel Histone Modification, by Jumonji Domain Containing 6 (JMJD6), J. Biol. Chem., 10.1074/jbc.M112.433284, 288, 9, 6053-6062, 2013.03.
42. Yamamoto, Y., Watanabe, T., Hoki, Y., Shirane, K., Li, Y., Ichiyanagi, K., Kuramochi-Miyagawa, S., Toyoda, A., Fujiyama, A., Oginuma, M., Suzuki, H., Sado, T., Nakano, T. & Sasaki, H., Targeted gene silencing in mouse germ cells by insertion of a homologous DNA into a piRNA generating locus, Genome Res., 10.1101/gr.137224.112, 23, 2, 292-299, 2013.02, レトロトランスポゾンはRNAを中間体として転移、増幅を繰り返すゲノムのパラサイト(寄生体)である。哺乳動物の生殖細胞ではPIWI-interacting RNA(piRNA)とよばれる小分子RNAがレトロトランスポゾンの活性を抑制している。今回、我々は生殖細胞のpiRNA経路のマシナリーを遺伝子抑制ツールとして利用できるかどうか調べた。パキテン期piRNAを産生するゲノム領域へ外来性DNA断片を挿入したマウスを作製し、この断片がpiRNAとなることを確認した。このマウスに、外来性DNAと相同な配列をもつレポーター遺伝子を有するマウスを交配すると、レポーター遺伝子の活性が顕著に抑制された。すなわち、パキテン期piRNA経路を利用した標的遺伝子の制御が可能であることを示した。.
43. Ichiyanagi, T., Ichiyanagi, K., Miyake, M. & Sasaki, H., Accumulation and loss of asymmetric non-CpG methylation during male germ-cell development, Nucl. Acids Res., 10.1093/nar/gks1117, 41, 2, 738-745, 2013.01.
44. Liu, Y., Toh, H., Sasaki, H., Zhang, X. & Cheng, X., An atomic model of ZFP57 recognition of CpG methylation within a specific DNA sequence., Genes Dev. 26, 2374-2379 , 2012.09.
45. Takahashi, T., Matsuzaki, H., Tomizawa, S., Okamura, E., Fukamizu, A., Sasaki, H. & Tanimoto, K. , Sequences in the H19 ICR that are transcribed as small RNA in oocytes are dispensable for methylation imprinting in YAC transgenic mice., Gene 508, 26-34, 2012.08.
46. Okae, H., Hiura, H., Miyauchi, N., Abe, C., Nishida, Y., Funayama, R., Tanaka, S., Chiba, H., Yaegashi, N., Nakayama, K., Sasaki, H. & Arima, T., Re-investigation and RNA sequencing-based identification of genes with placenta-specific imprinted expression, Hum. Mol. Genet. 21,548-558, 2012.02.
47. Ohnishi, Y., Totoki, Y., Toyoda, A., Watanabe, T., Yamamoto, Y., Tokunaga, K., Sakaki, Y., Sasaki, H. & Hohjoh, H., Active role of small non-coding RNAs derived from SINE/B1 retrotransposon during early mouse development., Mol. Biol. Rep. 39, 903-909, 2011.07.
48. Ichiyanagi, K., Li, Y., Watanabe, T., Ichiyanagi, T., Fukuda, K., Kitayama, J., Yamamoto, Y., Kuramochi-Miyagawa, S., Nakano, T., Yabuta, Y., Seki, Y., Saitou, M. & Sasaki, H., Locus- and domain-dependent control of DNA methylation at mouse B1 retrotransposons during male germ cell development, Genome Res. 21, 2058-2066, 2011.12.
49. Hoki, Y., Ikeda, R., Mise, N., Sakata, Y., Ohhata, T., Sasaki, H., Abe, K., and Sado, T. , Incomplete X-inactivation initiated by a hypomorphic Xist allele in the mouse
, Development 138, 2649-2659 , 2011.07.
50. Watanabe, T., Tomizawa, S., Mitsuya, K., Totoki, Y., Yamamoto, Y., Kuramochi-Miyagawa, S., Iida, N., Hoki, Y., Murphy, P.J., Toyoda, A., Gotoh, K., Hiura, H., Arima, T., Fujiyama, A., Sado, T., Shibata, T., Nakano, T., Lin, H., Ichiyanagi, K., Soloway, P.D. & Sasaki, H., Role for piRNAs and non-coding RNA in de novo DNA methylation of the imprinted mouse Rasgrf1 locus., Science 332, 848-852, 2011.05.
51. Tomizawa, S., Kobayashi, H., Watanabe, T., Andrews, S., Hata, K., Kelsey, G. & Sasaki, H., Dynamic stage-specific changes in imprinted differentially methylated regions during early mammalian development and prevalence of non-CpG methylation in oocytes., Development 138, 811-820, 2011.01.
52. Borgel, J., Guibert, S., Li, Y., Chiba, H., Schuebeler, D., Sasaki, H., Forne, T. & Weber, M., Targets and dynamics of promoter DNA methylation during early mouse development, Nat. Genet. 42, 1093-1100, 2010.12.
53. Takashima, S., Takehashi, M., Lee, J., Chuma, S. Okano, M., Hata, K. Suetake, I., Nakatsuji, N., Miyoshi, H., Tajima, S., Tanaka, Y., Toyokuni, S., Sasaki, H., Kanatsu-Shinohara, M. & Shinohara, T., Abnormal DNA methyltransferase expression in mouse germline stem cells results in spermatogenesis defects, Biol. Reprod. 81, 155-164, 2009.02.
54. Xie, Z.-H., Huang, Y.-N., Chen, Z.-X., Riggs, A.D., Ding, J.-P., Gowher, H., Jeltsch, A., Sasaki, H., Hata K. & Xu, G.-L., Mutations in DNA methyltransferase DNMT3B in ICF syndrome affect its regulation by DNMT3L, Hum. Mol. Genet. 15, 1375-1385, 2006.05.
55. Plass, C., Shibata, H., Kalcheva, I., Mullins, L., Kotelevtseva, N., Mullins, J., Kato, R., Sasaki, H., Hirotsune, S., Okazaki, Y., Held, W.A., Hayashizaki, Y. & Chapman, V.M., Identification of Grf1 on mouse chromosome 9 as an imprinted gene by RLGS-M, Nature Genet. 14, 106-109, 10.1038/ng0996-106, 14, 1, 106-109, 1996.09.
56. Hayashizaki, Y., Shibata, H., Hirotsune, S., Sugino, H., Okazaki, Y., Sasaki, N., Hirose, K., Imoto, H., Okuizumi, H., Muramatsu, M., Komatsubara, H., Shiroishi, T., Moriwaki, K., Matsuki, M., Hatano, N., Sasaki, H., Ueda, T., Mise, N., Takagi, N., Plass, C. & Chapman, V.M., Identification and characterization of an imprinted U2af binding protein related sequence on mouse chromosome 11 detected by efficient genomic screening using restriction landmark genomic scanning (RLGS-M), Nature Genet. 6, 33-40, 1994.01.
57. Izumi, R., Yamada, T., Yoshikai, S., Sasaki, H., Hattori, M. & Sakaki, Y., Positive and negative regulatory elements for the expression of the Alzheimer’s disease amyloid precursor-encoding gene in mouse, Gene 112, 189-195, 10.1016/0378-1119(92)90375-Y, 112, 2, 189-195, 1992.03.
58. Yamada, T. Araki, E., Izumi, R., Goto, I., Sasaki, H. & Sakaki, Y., Expression of Alzheimer amyloid beta-protein precursor gene in neuronal cells, Gerontology 37, 24-30, 37, 24-30, 1991.12.
59. Yoshikai, S., Sasaki, H., Doh-ura, K., Furuya, H. & Sakaki, Y., Genomic organization of the human amyloid beta-protein precursor gene, Gene 102, 291-292, 1991.06.
60. Oda, N., Ogawa, T., Ohno, M., Sasaki, H., Sakaki, Y. & Kihara, H., Cloning and sequence analysis of cDNA for Trimeresurus flavoviridis phospholipase A2 and consequent revision of the amino acid sequence, J. Biochem. 108, 816-821, 108, 5, 816-821, 1990.11.
61. Yoshikai, S., Sasaki, H., Doh-ura, K., Furuya, H. & Sakaki, Y., Genomic organization of the human amyloid beta-protein precursor gene, Gene 87, 257-263, 10.1016/0378-1119(90)90310-N, 87, 2, 257-263, 1990.03.
62. Yamada, T., Sasaki, H., Doh-ura, K., Goto, I. & Sakaki, Y., Structure and expression of the alternatively spliced forms of mRNA for the mouse homolog of Alzheimer's disease amyloid β protein precursor, Biochem. Biophys. Res. Commun. 158, 906-912, 10.1016/0006-291X(89)92808-8, 158, 3, 906-912, 1989.02.
63. Furuya, H., Sasaki, H., Goto, I., Wong, C. W., Glenner, G. G. & Sakaki, Y., Amyloid b protein gene duplication is not common in Alzheimer's disease: analysis by polymorphic restriction fragments, Biochem. Biophys. Res. Commun. 150, 75-81, 10.1016/0006-291X(88)90488-3, 150, 1, 75-81, 1988.01.
64. Yamada, T., Sasaki,H., Furuya, H., Miyata, T., Goto, I. & Sakaki, Y., Complementary DNA for the mouse homolog of the human amyloid b protein precursor, Biochem. Biophys. Res. Commun. 149, 665-671, 10.1016/0006-291X(87)90419-0, 149, 2, 665-671, 1987.12.
65. Sasaki, H., Oishi, N., Furuya, H., Yoshioka, K., Yamada, T., Ogawa, S., Wong, C. W., Glenner, G. G. & Sakaki, Y., A HindIII polymorphism detected by the cDNA encoding amyloid b protein of Alzheimer's disease, Nucl. Acids Res. 15, 6309, 10.1093/nar/15.15.6309, 15, 15, 6309-6309, 1987.08.