Kyushu University Academic Staff Educational and Research Activities Database
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Shu Takakura Last modified date:2019.06.10

Assistant Professor / Department of Psychosomatic Medicine
Psychosomatic Medicine
Kyushu University Hospital


Graduate School
Other Organization
Administration Post
Other
Other
Other


Homepage
http://edsupport-fukuoka.jp
Fukuoka Prefectural Treatment and Support Center for Eating Disorders .
Phone
092-642-5318
Fax
092-642-5336
Academic Degree
Medical science
Country of degree conferring institution (Overseas)
No
Field of Specialization
Psychosomatic medicine, Eating disorders, Thyroid disease, Molecular biology
Total Priod of education and research career in the foreign country
00years00months
Outline Activities
Psychosomatic medicine, Clinical and basic research on eating disorders
Research
Research Interests
  • Development of standard treatment for eating disorders
    keyword : eating disorders
    2015.04~2019.03.
  • Two-month Hospitalization Program for Patients with Anorexia Nervosa- A Prospective Study-
    keyword : Two-month hospitalization, anorexia nervosa, prospective study
    2008.04~2011.03.
  • Long-term starvation stress changes expression pattern of miRNA in mice brain
    keyword : eating disorders, anorexia nervosa, miRNA
    2009.04~2015.03.
Academic Activities
Papers
1. Tomokazu Hata, Yasuhiro Asano, Kazufumi Yoshihara, Tae Todani-Kimura, Noriyuki Miyata, Xue-Ting Zhang, Shu Takakura, Yuji Aiba, Yasuhiro Koga, Nobuyuki Sudo, Regulation of gut luminal serotonin by commensal microbiota in mice, PLoS One, 12, 7, 2017.07.
2. Keisuke Kawai, Megumi Nakashima, Masayasu Kojima, Sakino Yamashita, Shu Takakura, Miki Shimiizu, Chiharu Kubo, Nobuyuki Sudo, Ghrelin activation and neuropeptide Y elevation in response to medium chain triglyceride administration in anorexia nervosa patients, Clin Nutr ESPEN, 2017.02.
3. Chihiro Morita, Hirokazu Tsuji, Tomokazu Hata, Motoharu Gondo, Shu Takakura, Keisuke Kawai, Kazufumi Yoshihara, Kiyohito Ogata, Koji Nomoto, Kouji Miyazaki, Nobuyuki Sudo, Gut Dysbiosis in Patients with Anorexia, 10, 12, 2015.12.
4. Shu Takakura, Hiroaki Yokoyama, Chie Suzuyama, Keita Tatsushima, Makoto Yamashita, Chihiro Morita, Tomokazu Hata, Masato Takii, Keisuke Kawai, Nobuyuki Sudo, Three cases of appendicitis with anorexia nervosa under inpatient care, Journal of Eating Disorders, 3, 38, 2015.11.
5. Sakino Yamashita, Keisuke Kawai, Gen Komaki, Miki Shimizu, Megumi Nakashima, Sanami Eto, Shu Takakura, Masato Takii, Chiharu Kubo, Nobuyuki SUDO, The outcome of treatment for anorexia nervosa inpatients who required urgent hospitalization., BioPsychoSocial Medicine, 2014.09.
6. Kawai K, Yamashita S, Ymanaka T, Gondo M, Morita C, Nozaki T, Takakura S, Hata T, Yamada Y, Matsubayashi S, Takii M, Kubo C, Sudo N, The longitudinal BMI pattern and body composition of patients with anorexia nervosa who require urgent hospitalization: A case control study, BioPsychhoSocial Medicine, 5, 14, 2011.05.
7. Arimura C, Nozaki T, Takakura S, Kawai K, Takii M, Sudo N, Kubo C., Predictors of menstrual resumption by patients with anorexia nervosa, Eating and Weight Disorders, 2010.05.
8. Ujifuku K, Mitsutake N, Takakura S, Matsuse M, Saenko V, Suzuki K, Hayashi K, Matsuo T, Kamada K, Nagata I, Yamashita S., miR-195, miR-455-3p and miR-10a( *) are implicated in acquired temozolomide resistance in glioblastoma multiforme cells, Cancer Letters, 2010.05.
9. Takakura S, Mitsutake N, Nakashima M, Namba H, Saenko VA, Rogounovitch TI, Nakazawa Y, Hayashi T, Ohotusuru A, Yamashita S, Oncogenic role of miR-17-92 cluster in anaplastic thyroid cancer cells, Cancer Science, 99, 6, 1147-1154, 2008.06.
10. Kumagai A, Namba H, Takakura S, Inamasu E, Saenko VA, Ohtsuru A, Yamashita S., No evidence of ARAF, CRAF and MET mutations in BRAFT1799A negative human papillary thyroid carcinoma, Endocrine Journal, 53, 5, 615-20, 2006.08.
11. Takakura S, Nozaki M, Nomura Y, Koreeda C, Urabe H, Kawai K, Takii M, Kubo C, Factors related to renal dysfunction in patitents with anorexia nervosa, Eating and Weight Disorders, 11, 73-77, 2006.06.
12. Nozaki T, Takao M, Takakura S, Koreeda-Arimura C, Ishido K, Yamada Y, Kawai K, Takii M, Kubo C, Psychopathologial features of patients with prolonged anorexia nervosa as assessed by the Minnesota Multiphasic Personality Inventry (MMPI), Eating and Weight Disorders, 11, 59-65, 2006.06.
13. Bulgin D, Podtcheko A, Takakura S, Mitsutake N, Namba H, Saenko V, Ohtsuru A, Rogounovitch T, Palona I, Yamashita S., Selective pharmacologic inhibition of c-Jun NH2-terminal kinase radiosensitizes thyroid anaplastic cancer cell lines via induction of terminal growth arrest, Thyroid, 16, 3, 217-24, 2006.03.
Presentations
1. Shu Takakura, Hiroaki Yokoyama, Chie Suzuyama, Keita Tatsushima, Makoto Yamashita, Chihiro Morita, Tomokazu Hata, Masato Takii, Keisuke Kawai, Nobuyuki SUDO, Appendicitis with Anorexia Nervosa under Weight Gain, 23RD World Congress on Psychosomatic Medicine, 2015.08.
2. Shu Takakura, Specific miRNA expressions under chronic starvation stress in mouse hippocampus, The ICPM 22nd World Congress, 2013.09, Recently, DNA methylation abnormalities in anorexia nervosa (AN) patients were reported, which were suggesting epigenetic gene regulation. Therefore, in this study, we hypothesized that microRNAs (miRNAs), consists of 18-20 nucleotides and regulate translation of messenger RNAs (mRNAs), were altered under chronic starvation stress and played important roles in the mechanism of various abnormalities in AN patients.
We used 9-week BALB/c female mice and divided into five groups: control, 40% dietary restriction, 50% dietary restriction, 60% dietary restriction group and water avoidance chronic stress group. After twenty-one days dietary restriction, or 10 days (1 hour /day) water avoidance stress, we extracted total RNA from mouse hippocampus for microarray analysis and further examinations.
We observed that mortality rate was increased in the 50% and 60% restriction groups, in contrast that of 40% group was quite low. Therefore, we used 40% restriction group as a model of chronic starvation stress and for further examinations. Microarray analysis revealed that miR-1 was most down-regulated and miR-7 was most up-regulatted among 20 significantly altered miRNAs under starvation stress condition compared to the control. We compared expression levels of those miRNAs to those of water avoidance stress group using real-time RT-PCR. We present several chronic starvation stress specific miRNAs and putative target mRNAs. Putative target of such miRNAs were several mRNAs related to cell growth or neurogenesis.
In the current study, we found different miRNAs were altered and might play important roles under chronic starvation stress.
Those findings are quite novel and might be important to understand pathological mechanisms of AN from the viewpoint of epigenetics.
.
3.  Anorexia nervosa (AN) is one of diseases psychologically and physically severe which is difficult to treat. In AN patients, we observe various psycho-behavioral and physical abnormalities, however most mechanism of such abnormalities have been still unknown. Recently, DNA methylation abnormalities in AN patients were reported, which were suggesting epigenetic gene regulation. Therefore, in this study, we hypothesized that microRNAs (miRNAs), consists of 18-20 nucleotides and regulate translation of messenger RNAs (mRNAs), were altered under chronic starvation stress and played important roles in the mechanism of various abnormalities in AN patients.
We used 9-week BALB/c female mice and divided into four groups: control, 40% dietary restriction, 50% dietary restriction and 60% dietary restriction group. After twenty-one days dietary restriction, we extracted total RNA from mouse hippocampus for microarray analysis and further examinations.
We observed that mortality rate was increased in the 50% and 60% restriction groups, in contrast that of 40% group was quite low. Therefore, we used 40% restriction group as a model of chronic starvation stress and for further examinations. Microarray analysis using total RNA from mouse hippocampus revealed that expressions of several miRNAs were significantly altered under starvation stress condition compared to control group. Among significantly down- or up-regulated miRNAs, we focused on miR-1 and miR-7 whose manifestations were most down- and up-regulated. Real-time reverse transcription PCR analysis revealed that miR-7 was down-regulated in the 40% restriction group compared to control group. Putative target of miR-7 searched with Target Scan were several mRNAs related to cell growth or neurogenesis.
In the current study, we found many different miRNAs were altered and might play important roles under chronic starvation stress.
Those findings are quite novel and might be important to understand pathological mechanisms of AN from the viewpoint of epigenetics.
.
4. Two-Month Hospitalization Program for Patients with Anorexia Nervosa - A Prospective Study-.
Membership in Academic Society
  • Academy for Eating Disorders
  • Japanese Society of Behavioral Medicine
  • Japanese Society of Psychosomatic Internal Medicine
  • The Japanese Society of Internal Medicine
  • Japanese Society of Psycosomatic Medicine
  • Japan Society of Eating Disorders
  • Japan Thyroid Association
  • The Japan Endocrine Society
Awards
  • Chronic starvation stress alters miRNA expressions
Educational
Other Educational Activities
  • 2017.04.
  • 2016.04.
  • 2015.04.
Social
Professional and Outreach Activities
Fukuoka Prefectural Treatment and Support Center for Eating Disorders.