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Bungo Shirouchi Last modified date:2019.09.04

Assistant Professor / Food Science & Technology
Department of Bioscience and Biotechnology
Faculty of Agriculture


Graduate School
Undergraduate School


Academic Degree
Ph. D. (Agriculture)
Country of degree conferring institution (Overseas)
No
Field of Specialization
Nutrition Chemistry
ORCID(Open Researcher and Contributor ID)
orcid.org/0000-0003-4237-5081
Total Priod of education and research career in the foreign country
00years00months
Outline Activities
<Outline of research>
We are investigating how dietary factors influence our body. Main purpose of our study is to clarify the way to keep us healthy and best condition via modifying our dietary habit.
It consists of eight major themes as follows.
1. Effects of exogenous and endogenous oxycholesterols on lipid metabolism
2. Effects of food ingredients on development of obesity
3. Analysis of relation between serum fatty acid composition and disease
4. Identification of responsible genes for hypercholesterolemia of disease model animals
5. Epigenetic regulation of gene expression by dietary intervention
6. Effects of food ingredients on development of dyslipidemia and arteriosclerosis
7. Effects of food ingredients on lymphatic lipid transport
8. Research and development of rapid and sensitive micro-assay for the determination of rice lipids
Research
Research Interests
  • Effects of exogenous and endogenous oxycholesterols on various diseases
    keyword : Oxycholesterols, Lipid metabolism
    2010.03~2020.03.
  • Studies on regulation of lipid metabolism and alleviation of atherosclerosis with dietary components
    keyword : Lipid metabolism, Atherosclerosis, Dietary components
    2010.03~2020.03.
  • Analysis of relation between serum fatty acid composition and disease
    keyword : Serum fatty acid composition, Epidemiological study
    2010.03~2020.03.
  • Studies on prevention and alleviation of obesity with dietary components
    keyword : Obesity, Metabolic syndrome, Dietary components
    2010.03~2020.03.
  • Effects of dietary components on portal vein and lymphatic absorption
    keyword : Intestinal absorption, Portal vein, Lymph duct, Dietary lipids (Phospholipids)
    2010.03~2020.03.
Academic Activities
Books
1. Shirouchi B, Nagao K, and Yanagita T, Docosahexaenoic acid containing phosphatidylcholine alleviates obesity-related disorders in obese rats. Omega-3 Oils: Applications in Functional Foods, ed. by EM. Hernandez, M. Hosokawa, AOCS press, USA, p205-212, 2011.05.
2. Shirouchi B, Nagao K, and Yanagita T, Dietary phosphatidylinositol in metabolic syndrome. Biocatalysis and Biomolecular Engineering, ed. by C.T. Hou, J.F. Shaw, John Wiley & Sons, Inc., USA, p165-174, 2010.05.
Reports
1. Evaluation of Dietary Lipid Absorption under Physiological Conditions.
2. Physiological Significance of Oxidized Cholesterols.
3. Anti-metabolic syndrome effect by probiotics.
Papers
1. Akane Kanasaki, Zhe Jiang, Takuya Mizokami, Bungo Shirouchi, Tetsuo Iida, Yasuo Nagata, Masao Sato., Dietary D-allulose alters cholesterol metabolism in Golden Syrian hamsters partly by reducing serum PCSK9 levels., Journal of functional foods, 10.1016/j.jff.2019.103429, Accepted 18 Jun 2019., 2019.07.
2. Bungo Shirouchi, Ryosuke Matsuoka, Alleviation of Metabolic Syndrome with Dietary Egg White Protein, Journal of Oleo Science, 10.5650/jos.ess19084, 68, 6, 517-524, 2019.06, [URL], Abdominal fat accumulation causes metabolic syndrome, which is a cluster of metabolic abnormalities such as dyslipidemia, glucose intolerance, insulin resistance or hyperinsulinemia, and hypertension, leading to the development of diabetes and cardiovascular disease. Diets are known to contribute to the development or prevention of metabolic syndrome. Several studies have reported that the quality of dietary proteins may be an important modulator of the risk of this syndrome. We investigated the effects of consuming egg white protein (EWP) or lactic-fermented egg white (LE), an easy-to-consume form of egg white, on the development of metabolic syndrome in animal models and humans. In comparison with casein, dietary EWP decreased lymphatic lipid transport in thoracic lymph duct-cannulated rats. In an in vitro experiment, EWP pepsin hydrolysate decreased the cholesterol micellar solubility and cholesterol transfer rate from micelles to oil phase, and increased water-holding capacity, settling volume in water, and relative viscosity compared with casein pepsin hydrolysate. The daily consumption of LE for 8 weeks reduced serum total cholesterol and LDL cholesterol levels in men with mild hypercholesterolemia. Furthermore, dietary EWP reduced the body fat mass of rats by increasing the body protein mass and accelerating hepatic β-oxidation. The daily consumption of LE for 12 weeks reduced the visceral fat area and improved the ratio of the visceral to subcutaneous fat area. Taken together, these results indicated that dietary EWP and LE would be useful for preventing or alleviating metabolic syndrome..
3. Bungo Shirouchi, Yumiko Furukawa, Yuri Nakamura, Asuka Kawauchi, Katsumi Imaizumi, Hirosuke Oku, Masao Sato, Inhibition of Niemann-Pick C1 Like 1 by ezetimibe reduces dietary 5β,6β-epoxycholesterol absorption in rats., Cardiovascular Drugs and Therapy, 10.1007/s10557-019-06854-4., 33, 1, 35-44, 2019.01, [URL].
4. Misato Niibo*, Bungo Shirouchi*, Minami Umegatani, Yukiko Morita, Akihiro Ogawa, Fumihiko Sakai, Yukio Kadooka, Masao Sato (*These authors contributed equally.), Probiotic Lactobacillus gasseri SBT2055 improves insulin secretion in a diabetic rat model., Journal of Dairy Science, 10.3168/jds.2018-15203, Available online 22 November 2018, 2018.11, [URL], The probiotic Lactobacillus gasseri SBT2055 (LG2055) has a protective effect against metabolic syndrome in rats and humans. Metabolic syndrome increases the risk of type 2 diabetes mellitus. In this study, Goto-Kakizaki rats were used as a diabetic model and fed diets containing LG2055-fermented or nonfermented skim milk for 4 wk. Indices of diabetes such as blood glucose levels, serum glucagon levels, plasma levels of insulin, C-peptide, and glucagon-like peptide-1, tissue glycogen contents, and pancreatic mRNA levels were measured. The plasma C-peptide levels and pancreatic mRNA levels of insulin genes (Ins1 and Ins2) and Pdx1 (a transcriptional factor of insulin genes) were increased in LG2055 diet-fed rats. The increase in insulin secretion corresponded to an improvement in serum and pancreatic inflammatory status, associated with decreases in serum levels of serum amyloid P and pancreatic levels of granulocyte colony-stimulating factor. Insulin resistance in Goto-Kakizaki rats was ameliorated by increased glycogen storage in the liver and quadriceps femoris muscles and decreased serum free fatty acid levels. This improvement may be related to the increased cecal production of short-chain fatty acids. In conclusion, dietary LG2055 improved insulin secretion in diabetic rats by improving the inflammatory status in the pancreas and serum..
5. Bungo Shirouchi, Rikako Yamanaka, Shoji Tanaka, Fagyun Kawatou, Takuya Hayashi, Ai Takeyama, Akiko Nakao, Ryoko Goromaru, Masako Iwamoto, Masao Sato., Quantities of phospholipid molecular classes in Japanese meals and prediction of their sources by multiple regression analysis., Journal of Nutritional Science and Vitaminology, 10.3177/jnsv.64.215, 64, 3, 215-221, 2018.05, [URL], Dietary intake of total phospholipids (PLs) accounts for approximately 10% of total dietary lipids. Each PL molecular class has various beneficial effects on health. However, limited information is available regarding the intake of phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylinositol (PI), phosphatidylserine (PS), lysophosphatidylcholine (LPC) and sphingomyelin (SM) among Japanese people, and the relevant food sources. In this study, we quantified the contents of PC, PE, PI, PS, LPC, and SM in 120 meal samples served in a Japanese company's dormitory and cafeteria. Additionally, we measured the weight of each food group and estimated the contents of nutrients in these meals. Furthermore, we conducted a stepwise multiple regression analysis to identify predictors (food groups) of each PL class intake. The contents of total PL, PC, PE, PI+PS, LPC, and SM (mean value) were 4.44, 2.17, 0.632, 0.123, 0.313, and 0.127 g/d, respectively. These values were considered as daily PL intake in accordance with data (three macronutrients, vitamins, and minerals) from our study and the National Health and Nutrition Survey (NHNS) Japan, 2015. The content of eggs, meat, fish and shellfish, milk, pulses, fruits, mushrooms, cereals, and fats and oils in the meals predicted the PL and PC contents. The content of eggs, pulses, and mushrooms in the meals predicted the PE contents. Our results determined the daily intake of PL molecular classes among Japanese people and the food sources of PC and PE, and suggested that multiple regression analysis is useful for the prediction of food sources of bioactive components..
6. Yasutake Tanaka, Kiyomi Takahashi, Jun-ichi Kato, Ai Sawazaki, Taiki Akasaka, Naoko Fujita, Toshihiro Kumamaru, Yuhi Saito, Bungo Shirouchi, Masao Sato., Starch synthase (SS) IIIa and starch branching enzyme (BE) IIb-deficient mutant rice line ameliorates pancreatic insulin secretion in rats: Screening and evaluating mutant rice lines with anti-diabetic functionalities., British Journal of Nutrition, 10.1017/S0007114518000314, 119, 9, 970-980, 2018.05, [URL], Diabetes mellitus is a metabolic disease spreading worldwide that has been reported to worsen the development and progression of other diseases (cancer, vascular diseases and dementia). To establish functional rice lines with anti-postprandial hyperglycaemic effects, we developed mutant rice lines, which lack one or two gene(s) related to starch synthesis, and evaluated their effects. Powder of mutant rice lines or other grains was loaded to rats fasted overnight (oral grain powder loading test). Incremental area under time-concentration curves (iAUC) were calculated with monitored blood glucose levels. Rice lines with anti-postprandial hyperglycaemic effects were separated by cluster analysis with calculated iAUC. A double mutant rice #4019 (starch synthase IIIa (ss3a)/branching enzyme IIb (be2b)), one of the screened mutant rice lines, was fed to Goto-Kakizaki (GK) rats, an animal model for type 2 diabetes, for 5 weeks. Plasma levels of C-peptide, a marker of pancreatic insulin secretion, were measured with ELISA. For in vitro study, a rat pancreatic cell line was cultured with a medium containing rat serum which was sampled from rats fed #4019 diet for 2 d. After 24-h of incubation, an insulin secretion test was performed. Through the oral rice powder loading test, seven rice lines were identified as antidiabetic rice lines. The intake of #4019 diet increased plasma C-peptide levels of GK rats. This result was also observed in vitro. In rat serum added to cell medium, ornithine was significantly increased by the intake of #4019. In conclusion, the mutant rice #4019 promoted pancreatic insulin secretion via elevation of serum ornithine levels..
7. Junko Nagamoto, Ai Sawazaki, Motonori Miyago, Bungo Shirouchi, Mitsukazu Sakata, Li-Tao Tong, Toshihiro Kumamaru, Masao Sato., Rapid semi-quantification of triacylglycerols, phosphatidylcholines, and free fatty acids in the rice bran of one rice grain., Journal of Integrative Agriculture (formerly Agriculture Sciences in China), http://www.chinaagrisci.com/Jwk_zgnykxen/EN/abstract/abstract11495.shtml, 17, 5, 977-981, 2018.05, [URL], We developed a microplate assay method for determining the contents of triacylglycerols (TAGs), phosphatidylcholines (PCs), and free fatty acids (FFAs) in the rice bran of one grain using enzymatic reactions. In this method, enzymes from commercially available kits were used. Optimum reaction conditions were established. It was found that Nonidet P-40 was the optimal among the three surfactants used (Triton X-100, Tween 40, and Nonidet P-40) when lipid was dissolved in a reaction solution. Using this method, it was possible to quantify TAGs, PCs, and FFAs in concentration ranges of 7–150, 5–70, and 8–200 mg L−1, respectively. Furthermore, when the TAG contents in the rice bran were measured, the values closely corresponded to those obtained by extracting from large amounts of rice bran. However, sufficient data on the PC and FFA contents in rice bran are not available for valid comparisons. Although this method can accurately quantify the TAG contents in the rice bran of one grain, the accuracy of the PC and FFA contents has not been verified. Hence, future study is necessary..
8. Ryosuke Matsuoka*, Bungo Shirouchi*, Minami Umegatani, Meguri Fukuda, Ayano Muto, Yasunobu Masuda, Masaaki Kunou, Masao Sato (*These authors contributed equally.)., Dietary egg-white protein increases body protein mass and reduces body fat mass through an acceleration of hepatic β-oxidation in rats., British Journal of Nutrition, 10.1017/S0007114517002306., 118, 6, 423-430, 2017.09, [URL], Egg-white protein (EWP) is known to reduce lymphatic TAG transport in rats. In this study, we investigated the effects of dietary EWP on body fat mass. Male rats, 4 weeks old, were fed diets containing either 20 % EWP or casein for 28 d. Carcass protein levels and gastrocnemius leg muscle weights in the EWP group were significantly higher than those in the casein group. In addition, carcass TAG levels and abdominal fat weights in the EWP group were significantly lower than those in the casein group; adipocyte size in abdominal fat in the EWP group was smaller than that in the casein group. To identify the involvement of dietary fat levels in the rats, one of two fat levels (5 or 10 %) was added to their diet along with the different protein sources (EWP and casein). Abdominal fat weight and serum and hepatic TAG levels were significantly lower in the EWP group than in the casein group. Moreover, significantly higher values of enzymatic activity related to β-oxidation in the liver were observed in the EWP group compared with the casein group. Finally, abdominal fat weight reduction in the EWP group with the 10 % fat diet was lower than that in the EWP group with the 5 % fat diet. In conclusion, our results indicate that, in addition to the inhibition of dietary TAG absorption reported previously, dietary EWP reduces body fat mass in rats through an increase of body protein mass and the acceleration of β-oxidation in the liver..
9. Bungo Shirouchi, Kentaro Kashima, Yasutaka Horiuchi, Yuki Nakamura, Yumiko Fujimoto, Li-Tao Tong, Masao Sato., 27-Hydroxycholesterol suppresses lipid accumulation by down-regulating lipogenic and adipogenic gene expression in 3T3-L1 cells., Cytotechnology, 10.1007/s10616-016-9962-5, 69, 3, 485-492, 2017.06, [URL], Cholesterol oxidation products (oxycholesterols) are produced from cholesterol by automatic and/or enzymatic oxidation of the steroidal backbone and side-chain. Oxycholesterols are present in plasma and serum, suggesting that oxycholesterols are related to the development and progression of various diseases. However, limited information is available about the absolute amounts of oxycholesterols in organs and tissues, and the physiological significance of oxycholesterols in the body. In the present study, we quantified the levels of 13 oxycholesterols in white adipose tissue (WAT) of mice and then evaluated correlations between each oxycholesterol level and WAT weight. The sum of the levels of 13 oxycholesterols in WAT (white adipose tissue) was 15.9 ± 3.4 μg/g of WAT weight and approximately 1 % of cholesterol level. Among oxycholesterols, the levels of 27-hydroxycholesterol (27-OH), an endogenous oxycholesterol produced by enzymatic oxidation, and the relative WAT weights were significantly negatively correlated. Next, we evaluated the effects of 27-OH on lipogenesis and adipogenesis in 3T3-L1 cells. TO901317 (TO), a potent and selective agonist for LXRα, significantly increased intracellular TAG contents, while 27-OH significantly reduced the contents to half when compared with control (DMSO) and completely abolished the effect of TO. In addition, 27-OH significantly reduced the mRNA levels of lipogenic (LXRα and FAS) and adipogenic genes (PPARγ and aP2) during adipocyte maturation of 3T3-L1 cells. In conclusion, our results indicate that 27-OH suppresses lipid accumulation by down-regulating lipogenic and adipogenic gene expression in 3T3-L1 cells..
10. Kayo Kurotani, Masao Sato, Kazuki Yasuda, Kentaro Kashima, Shoji Tanaka, Takuya Hayashi, Bungo Shirouchi, Shamima Akter, Ikuko Kashino, Hitomi Hayabuchi, Tetsuya Mizoue., Even- and odd-chain saturated fatty acids in serum phospholipids are differentially associated with adipokines., PLoS One, 10.1371/journal.pone.0178192, 12, 5, e0178192, 2017.05, [URL], BACKGROUND: Saturated fatty acids are generally thought to have detrimental effects on health. However, a recent study showed that even- and odd-chain saturated fatty acids had opposite associations with type 2 diabetes. Limited studies of Western populations examined the associations of circulating saturated fatty acids with adipokines, an important role in glucose metabolism.
OBJECTIVE: We examined the associations of saturated fatty acids in serum phospholipids with circulating levels of adipokines among a Japanese population.
DESIGN: A cross-sectional study was conducted among 484 Japanese employees (284 men and 200 women) aged 20-65 years. The serum fatty acid composition in the phospholipid fraction was measured by gas-chromatography. Serum leptin, adiponectin, plasminogen activator inhibitor-1 (PAI-1), resistin, and visfatin were measured using a Luminex suspension bead-based multiplexed array. Multiple linear regression analysis was performed to assess the association between saturated fatty acids and adipokines, with adjustment for potential confounding variables.
RESULTS: Even- and odd-chain saturated fatty acids were differentially associated with adipokines. Higher levels of even-chain saturated fatty acids (14:0 myristic, 16:0 palmitic, and 18:0 stearic acids) were associated with higher levels of resistin (P for trend = 0.048) and lower levels of adiponectin (P for trend = 0.003). By contrast, odd-chain saturated fatty acids (15:0 pentadecanoic and 17:0 heptadecanoic acids) showed inverse associations with leptin and PAI-1 (P for trend = 0.048 and 0.02, respectively). Visfatin was positively associated with both even- and odd-chain saturated fatty acids.
CONCLUSIONS: The results suggest that even- and odd-chain saturated fatty acids are differentially associated with adipokine profile..
11. Bungo Shirouchi, Koji Nagao, Minami Umegatani, Aya Shiraishi, Yukiko Morita, Shunichi Kai, Teruyoshi Yanagita, Akihiro Ogawa, Yukio Kadooka, Masao Sato., Probiotic Lactobacillus gasseri SBT2055 improves glucose tolerance and reduces body weight gain in rats by stimulating energy expenditure., British Journal of Nutrition, 10.1017/S0007114516002245, 116, 3, 451-458, 2016.08, [URL], Probiotic Lactobacillus gasseri SBT2055 (LG2055) reduces postprandial TAG absorption and exerts anti-obesity effects in rats and humans; however, the underlying mechanisms are not fully understood. In the present study, we addressed the mechanistic insights of the anti-obesity activity of LG2055 by feeding Sprague-Dawley rats diets containing skimmed milk fermented or not by LG2055 for 4 weeks and by analysing energy expenditure, glucose tolerance, the levels of SCFA in the caecum and serum inflammatory markers. Rats fed the LG2055-containing diet demonstrated significantly higher carbohydrate oxidation in the dark cycle (active phase for rats) compared with the control group, which resulted in a significant increase in energy expenditure. LG2055 significantly reduced cumulative blood glucose levels (AUC) compared with the control diet after 3 weeks and increased the molar ratio of butyrate:total SCFA in the caecum after 4 weeks. Furthermore, the LG2055-supplemented diet significantly reduced the levels of serum amyloid P component - an indicator of the inflammatory process. In conclusion, our results demonstrate that, in addition to the inhibition of dietary TAG absorption reported previously, the intake of probiotic LG2055 enhanced energy expenditure via carbohydrate oxidation, improved glucose tolerance and attenuated inflammation, suggesting multiple additive and/or synergistic actions underlying the anti-obesity effects exerted by LG2055..
12. Chisato Inoue, Tomomi Kozaki, Yukiko Morita, Bungo Shirouchi, Katsuya Fukami, Kuniyoshi Shimizu, Masao Sato, Yoshinori Katakura., Kibizu concentrated liquid suppresses the accumulation of lipid droplets in 3T3-L1 cells., Cytotechnology, 10.1007/s10616-015-9849-x, 67, 4, 721-725, 2015.08, [URL], Adipocyte size is closely related to the occurrence of diabetes, metabolic syndrome, and insulin resistance. Thus, researchers are searching for active substances that function to reduce adipocyte size. In the present study, we focused on sugar cane vinegar, Kibizu, and evaluated the function of Kibizu to reduce adipocyte size by using an in vitro model system, because people in Amami Oshima famous for longevity regularly consume Kibizu. Results showed that Kibizu treatment significantly reduced the size and number of lipid droplets in 3T3-L1 cells, relative to treatment with Kurozu, another traditional vinegar. Results of an extraction experiment suggest that the active components in Kibizu are lipophilic and hydrophobic. In addition, an in vivo experiment on rats treated with Kibizu showed that the active components were contained in large vein blood. Results of an additional in vivo experiment suggest that metabolites generated by Kibizu-treated rats are primarily contained or modified specifically in the large vein blood..
13. Ryosuke Matsuoka*, Bungo Shirouchi*, Sayaka Kawamura, Sanae Baba, Sawako Shiratake, Kazuko Nagata, Katsumi Imaizumi, Masao Sato (*These authors contributed equally.)., Dietary egg white protein inhibits lymphatic lipid transport in thoracic lymph duct-cannulated rats., Journal of Agricultural and Food Chemistry, 10.1021/jf502741b, 62, 44, 10694-10700, 2014.11, [URL], Dietary egg white protein (EWP) decreases serum cholesterol levels. We previously showed that EWP decreased cholesterol absorption in the intestine. Rats subjected to permanent lymph duct cannulation were used to investigate the effects of dietary EWP on lipid transport. They were fed diets with 20% EWP and casein, and their lymph was collected to quantify lymphatic lipid levels. Dietary EWP decreased lymphatic cholesterol transport compared with casein. We previously showed that EWP excluded cholesterol from bile acid micelles. Therefore, we prepared pepsin-hydrolyzed EWP and casein. EWP was not completely digested. Ovalbumin, which is the most abundant protein in EWP, showed resistance to digestion by pepsin. Here we investigated the effects of EWP pepsin hydrolysate (EWP-ph) on cholesterol micellar solubility, cholesterol transfer from the micellar to the oil phase, water-holding capacity (WHC), settling volume in water (SV), and relative viscosity and compared them with the effects of casein pepsin hydrolysate (C-ph). EWP-ph significantly decreased the micellar solubility and transfer rate and increased the WHC, SV, and relative viscosity compared with C-ph. Moreover, the pepsin hydrolysate of ovalbumin, a major protein in EWP, played a role in decreasing cholesterol micellar solubility and leading to the inhibition of cholesterol absorption. In conclusion, dietary EWP decreased cholesterol intestinal absorption by exerting combined effects of these physicochemical properties in the gut..
14. Kazuaki Yoshinaga, Keiichi Sasaki, Hiroyuki Watanabe, Koji Nagao, Nao Inoue, Bungo Shirouchi, Teruyoshi Yanagita, Toshiharu Nagai, Hoyo Mizobe, Koichi Kojima, Fumiaki Beppu, Naohiro Gotoh., Differential effects of triacylglycerol positional isomers containing n-3 series highly unsaturated fatty acids on lipid metabolism in C57BL/6J mice., The Journal of Nutritional Biochemistry, 10.1016/j.jnutbio.2014.09.004, 26, 1, 57-63, 2015.01, The present study investigated the effects of binding position of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) to triacylglycerol (TAG) on lipid metabolism in C57BL/6 J mice. Mice were treated with pure TAG positional isomers, including 1,2(2,3)-dipalmitoyl-3(1)-eicosapentaenoyl glycerol, 1,3-dipalmitoyl-2-eicosapentaenoyl glycerol, 1,2(2,3)-dipalmitoyl-3(1)-docosahexaenoyl glycerol, and 1,3-dipalmitoyl-2-docosahexaenoyl glycerol. Compared to DHA bound to the α-position of TAG, DHA bound to the β-position more effectively inhibited fatty-acid synthetic enzymes and cholesterol-metabolism enzymes and thus reduced TAG and cholesterol concentrations in the serum and liver. EPA bound to the α-position of TAG, but not EPA bound to the β-position of TAG, significantly decreased hepatic cholesterol concentrations. Additionally, EPA bound to the α-position of TAG increased the ratio of PGI2 to TXA2 to a higher degree than EPA bound to the β-position. These results suggested that the binding position of EPA and DHA to TAG affected TAG and cholesterol metabolism as well as eicosanoid production in C57BL/6 J mice..
15. Akihiro Ogawa, Yukio Kadooka, Ken Kato, Bungo Shirouchi, Masao Sato., Lactobacillus gasseri SBT2055 reduces postprandial and fasting serum non-esterified fatty acid levels in Japanese hypertriacylglycerolemic subjects., Lipids in Health and Disease, 10.1186/1476-511X-13-36, 13, 36, 2014.02, [URL], BACKGROUND: Lactobacillus gasseri SBT2055 (LG2055) inhibits dietary fat absorption in rats and exerts preventive effects on abdominal adiposity in rats and humans. The present study aimed to evaluate the effects of LG2055 on postprandial serum lipid responses in Japanese subjects with hypertriacylglycerolemia after the intake of oral fat-loading test (OFLT) meals.
METHODS: We conducted a single-blind, placebo-controlled, within-subject, repeated-measure intervention trial. Twenty subjects initially ingested the fermented milk (FM) without LG2055 for 4 weeks (control FM period), followed by a 4-week washout period, and then consumed FM containing LG2055 for 4 weeks (active FM period). The subjects were asked to consume FM at 200 g/day. At the end of each 4-week period, an 8-h OFLT was conducted. Blood samples were collected at fasting and every hour for 8 h after OFLT meal intake. Thereafter, postprandial serum non-esterified fatty acid (NEFA) and triacylglycerol (TAG) levels and fasting blood parameters were measured.
RESULTS: The OFLT showed that the postprandial serum NEFA levels from 120 to 480 min and the postprandial serum TAG level at 120 min in the active FM period were significantly (P < 0.05) lower than those in the control FM period. The fasting serum NEFA level in the active FM period significantly (P < 0.001) decreased at week 4 from the initial period compared with the control FM period.
CONCLUSIONS: The consumption of probiotic LG2055 reduced postprandial and fasting serum NEFA levels, suggesting its possible contribution to the reduction of the risk for obesity and type 2 diabetes mellitus. Trial registration: UMIN000011605..
16. Masao Sato, Yukiko Morita, Akihiro Ogawa, Yukio Kadooka, Bungo Shirouchi, EFFECTS OF DIETARY LACTOBACILLUS GASSERI SBT2055 ON GLUCOSE METABOLISM IN RATS., Annals of Nutrition and Metabolism, 10.1159/000354245, 63(suppl 1), 1632-1633, 2013.09, [URL].
17. Bungo Shirouchi, Yuri Nakamura, Yumiko Furukawa, Asuka Kawauchi, Katsumi Imaizumi, Masao Sato, DIETARY LIPID DYNAMICS IN THE THORACIC LYMPH REGULATED BY FOOD COMPONENTS AND DRUG IN THE INTESTINAL LUMEN., Annals of Nutrition and Metabolism, 10.1159/000354245, 63(suppl 1), 1261, 2013.09, [URL].
18. Teruyoshi Yanagita, Bungo Shirouchi, Koji Nagao, BENEFICIAL EFFECTS OF DIETARY PHOSPHOLIPIDS ON METABOLIC SYNDROME., Annals of Nutrition and Metabolism, 10.1159/000354245, 63(suppl 1), 59, 2013.09, [URL].
19. Masako Iwamoto, Kaori Yagi, Kayoko Yazumi, Airi Komine, Bungo Shirouchi, Masao Sato., Eating a healthy lunch improves serum alanine aminotransferase activity., Lipids in Health and Disease, 10.1186/1476-511X-12-134, 12, 134, 2013.09, [URL], BACKGROUND:
Nutritional guidance and diet control play important roles in the treatment of obesity and non-alcoholic fatty liver. However, in Japan, nutritional guidance is difficult to provide in practice. Therefore, we evaluated the effects of providing the 'once-a-day' intervention of a healthy lunch on various metabolic parameters.
METHODS:
For a 1-month preparatory period, 10 subjects generally consumed the lunches that were provided by the worksite cafeteria. This was followed by a 1-week washout period, after which, the subjects consumed healthy, low-calorie, well-balanced lunches for a 1-month test period. After the preparatory and test periods, blood samples were obtained from all subjects. The serum levels of indices relevant to metabolic syndrome and fatty liver were measured.
RESULTS:
Serum alanine aminotransferase activity significantly decreased by 20.3% after the healthy intervention. However, the indices of metabolic syndrome did not significantly change. Analysis of the relationship between serum alanine aminotransferase activity and nutrient content indicated that the improvement of serum alanine aminotransferase status was due to the higher vegetable content and lower animal-source protein of the meals provided.
CONCLUSIONS:
In summary, the 'once-a-day' intervention of providing a healthy lunch improved serum alanine aminotransferase status. A diet high in vegetables and low in animal-based protein is important in maintaining a healthy condition..
20. Effects of ezetimibe on lymphatic absorption of dietary oxycholesterols and its accumulation in body.
21. Bungo Shirouchi, Elke Albrecht, Gerd Nuernberg, Steffen Maak, Samadmanivong Olavanh, Yoshinori Nakamura, Takaufmi Gotoh, Masao Sato, Karin Nuernberg., Fatty acid profiles and adipogenic gene expression of various fat depots in Japanese Black and Holstein steers., Meat Science, 10.1016/j.meatsci.2013.06.027., 96, 1, 157-164, 2014.01.
22. Bungo Shirouchi, Yuri Nakamura, Yumiko Furukawa, Aya Shiraishi, Hiroko Tomoyori, Katsumi Imaizumi, Masao Sato, Ezetimibe inhibits lymphatic transport of esterified cholesterol but not free cholesterol in thoracic lymph duct-cannulated rats., Cardiovascular Drugs and Therapy, 10.1007/s10557-012-6403-3, 26, 5, 427-431, 2012.07, [URL], Purpose
Ezetimibe has been shown to inhibit dietary cholesterol absorption in animal models and humans, but studies on lymphatic lipid transport have not yet been performed. Rats subjected to permanent lymph duct cannulation were used to investigate the effects of ezetimibe on lipid transport.
Methods
Rats were fed diets with and without ezetimibe (5.0 mg/kg), and their lymph was collected after feeding to quantify lymphatic lipid levels. Total cholesterol content in the intestinal mucosa was also measured.
Results
Rats that consumed ezetimibe had significantly lower lymphatic total cholesterol transport with the reduction of esterified cholesterol transport. According to the calculation based on cholesterol consumption, ezetimibe reduced the total cholesterol lymphatic recovery rate by 54 %. We also determined that ezetimibe significantly reduced the total cholesterol content in the intestinal mucosa.
Conclusion
This is the first direct evidence that ezetimibe inhibits esterified but not free cholesterol lymphatic transport in thoracic duct-cannulated rats. The results also indicate that ezetimibe is not involved in the lymphatic transport of triacylglycerols, phospholipids, or α-tocopherol..
23. Masashi Inafuku, Koji Nagao, Saori Nomura, Bungo Shirouchi, Nao Inoue, Naoki Nagamori, Hideyuki Nakayama, Takayoshi Toda, Teruyoshi Yanagita., Protective effects of fractional extracts from Panellus serotinus on non-alcoholic fatty liver disease in obese, diabetic db/db mice., British Journal of Nutrition, 10.1017/S0007114511003485, 107, 5, 639-646, 2012.03.
24. Bungo Shirouchi, Sayaka Kawamura, Ryosuke Matsuoka, Sanae Baba, Kazuko Nagata, Sawako Shiratake, Hiroko Tomoyori, Katsumi Imaizumi, Masao Sato., Dietary guar gum reduces lymph flow and diminishes lipid transport in thoracic duct-cannulated rats., Lipids, 10.1007/s11745-011-3570-0, 46, 8, 789-793, 2011.08.
25. Masao Sato, Takatoshi Ueda, Kazuko Nagata, Sawako Shiratake, Hiroko Tomoyori, Mitsuo Kawakami, Yukio Ozaki, Hiroshi Okubo, Bungo Shirouchi, Katsumi Imaizumi., Dietary kakrol (Momordica dioica Roxb.) flesh inhibits triacylglycerol absorption and lowers the risk for development of fatty liver in rats., Experimenal Biology and Medicine, 10.1258/ebm.2011.011037, 236, 10, 1139-1146, 2011.07.
26. Nagao K, Yamano N, Shirouchi B, Inoue N, Murakami S, Sasaki T, Yanagita T., Effects of Citrus Auraptene (7-Geranyloxycoumarin) on Hepatic Lipid Metabolism in Vitro and in Vivo., J Agric Food Chem., 10.1021/jf1020329, 58, 16, 9028-9032, 2010.11.
27. Shirouchi B, Nagao K, Furuya K, Shiojiri M, Liu X, Yanagita T., Physiological effects of dietary soybean-derived phospholipid PIPS in obese Zucker (fa/fa) rats., Biosci Biotechnol Biochem., 10.1271/bbb.100408, 74, 11, 2333-2335, 2010.11.
28. Bungo Shirouchi, Koji Nagao, Kenta Furuya, Toshiharu Nagai, Kenji Ichioka, Shigeru Tokairin, Yasuhiro Iida, Teruyoshi Yanagita., Physiological Functions of iso-type Short-Chain Fatty Acid and Omega 3 Polyunsaturated Fatty Acids Containing Oil in Obese OLETF Rats., Journal of Oleo Science, 10.5650/jos.59.299, 59, 6, 299-305, 2010.06.
29. Nagao K, Inoue N, Inafuku M, Shirouchi B, Morooka T, Nomura S, Nagamori N, Yanagita T., Mukitake mushroom (Panellus serotinus) alleviates nonalcoholic fatty liver disease through the suppression of monocyte chemoattractant protein 1 production in db/db mice., J Nutr Biochem., 10.1016/j.jnutbio.2009.01.021, 21, 5, 418-423, 2010.05.
30. Gotoh N, Nagao K, Onoda S, Shirouchi B, Furuya K, Nagai T, Mizobe H, Ichioka K, Watanabe H, Yanagita T, Wada S., Effects of three different highly purified n-3 series highly unsaturated fatty acids on lipid metabolism in C57BL/KsJ-db/db mice., J Agric Food Chem., 10.1021/jf9026553, 57, 22, 11047-11054, 2009.11.
31. Shirouchi B, Nagao K, Koga S, Matsumoto H, Otsubo K, Nagata J, Yanagita T, DIETARY PHOSPHATIDYLINOSITOL PREVENTS METABOLIC SYNDROME IN OBESE, DIABETIC RATS, Ann. Nutr. Metab., 55, Suppl.1, 537-537, 2009.10.
32. Bungo Shirouchi, Koji Nagao, Kenta Furuya, Nao Inoue, Masashi Inafuku, Masaaki Nasu, Kazumasa Otsubo, Shinji Koga, Hideyuki Matsumoto, Teruyoshi Yanagita., Effect of dietary phosphatidylinositol on cholesterol metabolism in Zucker (fa/fa) rats., Journal of Oleo Science, 10.5650/jos.58.111, 58, 3, 111-115, 2009.03.
33. Bungo Shirouchi, Koji Nagao, Nao Inoue, Kenta Furuya, Shinji Koga, Hideyuki Matsumoto, Teruyoshi Yanagita., Dietary phosphatidylinositol prevents the development of nonalcoholic fatty liver disease in Zucker (fa/fa) rats., Journal of Agricultural and Food Chemistry, 10.1021/jf703578d, 56, 7, 2375-2379, 2008.04.
34. Bungo Shirouchi, Koji Nagao, Nao Inoue, Takeshi Ohkubo, Hidehiko Hibino, Teruyoshi Yanagita., Effect of dietary omega 3 phosphatidylcholine on obesity-related disorders in obese Otsuka Long-Evans Tokushima fatty rats., Journal of Agricultural and Food Chemistry, 10.1021/jf071225x, 55, 17, 7170-7176, 2007.08.
Presentations
1. Bungo Shirouchi, Alleviation of metabolic syndrome with dietary egg white protein, 日本油化学会第57回年会 JOCS-AOCS Joint Symposium, 2018.09, [URL].
2. Takuya Mizokami, Yasutake Tanaka, Bungo Shirouchi, Satoshi Ishizuka, Taizou Nagura, Michihiro Fukushima, Masao Sato, Effects of dietary trimethylglycine on cholesterol metabolism, AFELiSA (International Symposium on Agriculture, Food, Environmental and Life Science in Asia) 2017, 2017.11.
3. Asami Teramoto, Bungo Shirouchi, Masao Sato, Effects of dietary phosphatidylserine on gastric emptying and postprandial glycemic response in rats, AFELiSA (International Symposium on Agriculture, Food, Environmental and Life Science in Asia) 2017, 2017.11.
4. Ai Sawazaki, Yoshinori Katakura, Bungo Shirouchi, Masao Sato, Neural morphometric appraisal by addition of apolipoprotein E in human neuroblastoma cell line SH-SY5T, AFELiSA (International Symposium on Agriculture, Food, Environmental and Life Science in Asia) 2017, 2017.11.
5. Motonori Miyago, Yurika Miyazaki, Masahiro Ono, Bungo Shirouchi, Masao Sato, Cerebral cortical oxycholesterols as indices for dementia in the rats, AFELiSA (International Symposium on Agriculture, Food, Environmental and Life Science in Asia) 2017, 2017.11.
6. Misato Niibo, Bungo Shirouchi, Masao Sato, Development of hepatic disease model mice by inducing inflammation, AFELiSA (International Symposium on Agriculture, Food, Environmental and Life Science in Asia) 2017, 2017.11.
7. Yasutake Tanaka, Kiyomi Takahashi, Jun-ichi Kato, Taiki Akasaka, Naoko Fujita, Yuhi Saito, Bungo Shirouchi, Masao Sato, Screening of rice (Oryza sativa L.) grains with antidiabetic functionalities, ACN2015 (12th Asian Congress of Nutrition), 2015.05.
8. Bungo Shirouchi, Asuka Kawauchi, Yumiko Furukawa, Yuko Arima, Masao Sato, Effects of dietary phosphatidylcholine on postprandial lymphatic inflammatory cytokine levels in rats, ACN2015 (12th Asian Congress of Nutrition), 2015.05.
9. Bungo Shirouchi, Asuka Kawauchi, Yumiko Furukawa, Yuko Arima, Masao Sato, Dietary phosphatidylcholine reduces lymphatic inflammatory cytokine levels in thoracic lymph-duct cannulated rats, 1st Asian Conference on Oleo Science (ACOS2014), 2014.09.
10. Bungo Shirouchi, Ryuei Baba, Takuya Kato, Koji Nagao, Teruyoshi Yanagita, Masao Sato, Dietary Resveratrol Alleviates Atherosclerotic Lesions in ApoE-deficient Mice: Comparison with Pterostilbene, a Resveratrol Analog, 27th International Conference on Polyphenols & 8th Tannin Conference, 2014.09.
11. Bungo Shirouchi, Masao Sato, Lymphatic Lipid Transport Regulated by Dietary Components and Drug, 105th AOCS Annual Meeting & Expo, A Joint World Congress with the Japan Oil Chemists' Society, 2014.05.
12. Bungo Shirouchi, Final Report of the JSPS Strategic Young Researcher Overseas Visits Program for Accelerating Brain Circulation, 2014.02.
13. Masao Sato, Yukiko Morita, Akihiro Ogawa, Yukio Kadooka, Bungo Shirouchi, EFFECTS OF DIETARY LACTOBACILLUS GASSERI SBT2055 ON GLUCOSE METABOLISM IN RATS., IUNS 20th International Congress of Nutrition, 2013.09, [URL].
14. Bungo Shirouchi, Yuri Nakamura, Yumiko Furukawa, Asuka Kawauchi, Katsumi Imaizumi, Masao Sato, DIETARY LIPID DYNAMICS IN THE THORACIC LYMPH REGULATED BY FOOD COMPONENTS AND DRUG IN THE INTESTINAL LUMEN., IUNS 20th International Congress of Nutrition, 2013.09, [URL].
15. Teruyoshi Yanagita, Bungo Shirouchi, Koji Nagao, BENEFICIAL EFFECTS OF DIETARY PHOSPHOLIPIDS ON METABOLIC SYNDROME., IUNS 20th International Congress of Nutrition, 2013.09, [URL].
16. Effects of ezetimibe on lymphatic absorption of dietary oxycholesterols and its accumulation in body.
17. Dietary guar gum reduces lymph flow and diminishes lipid transport in thoracic duct-cannulated rats..
18. Research for serum fatty acid indices of improvement in metabolic syndrome..
19. Effects of Lactobacillus gasseri SBT2055 on glucose metabolism in diabetic rats.
Membership in Academic Society
  • Lipid Hydroperoxide Biology and Medicine
  • Japan Oil Chemists' Society
  • Japanese Society of Nutrition and Food Science
  • Japan Society for Bioscience, Biotechnology, and Agrochemistry