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Kaori Tabata Last modified date:2019.05.27

Assistant Professor / Division of Molecular Bioinformatics
Department of Chemo-Pharmaceutical Sciences
Faculty of Pharmaceutical Sciences


Graduate School
Undergraduate School


E-Mail
Academic Degree
Ph.D.
Field of Specialization
molecular biology, protein science
Research
Research Interests
  • Structural Basis for synthesis of tropane by polyketide synthase from Datura
    keyword : polyketide synthase
    2014.04.
  • Studies on the mechanism of cell death induced by cannabinoids
    keyword : cell death
    2013.01.
  • Structure and function analysis of heparanase involved in cancer metastasis
    keyword : structural analysis
    2010.06.
  • Silkworm expression of immune cell surface receptor
    keyword : protein science
    2007.04.
  • Structure and function of DNA replication related protein in Escherichia coli
    keyword : structural analysis
    2003.04.
  • Studies on the metabolic enzyme from medicinal plants
    keyword : enzyme, cloning
    2000.04.
Academic Activities
Papers
1. Sasaki K, Taura F, Shoyama Y, Morimoto S, Molecular characterization of a novel beta-glucuronidase from Scutellaria baicalensis georgi, J. Biol. Chem., 275, 35, 27466-27472, 2000.09.
2. Sasaki K, Ose T, Tanaka T, Mizukoshi T, Ishigaki T, Maenaka K, Masai H, Kohda D, Crystallization and preliminary crystallographic analysis of the N-terminal domain of PriA from Escherichia coli, Biochim. Biophys. Acta, 1764, 1, 157-160 , 2006.01.
3. Sasaki K, Ose T, Okamoto N, Maenaka K, Tanaka T, Masai H, Saito M, Shirai T, Kohda D, Structural basis of the 3'-end recognition of a leading strand in stalled replication forks by PriA, EMBO J., 26, 10, 2584-2593 , 2007.05.
4. Sasaki K, Kajikawa M, Kuroki K, Motohashi T, Shimojima T, Park EY, Kondo S, Yagi H, Kato K, Maenaka K, Silkworm expression and sugar profiling of human immune cell surface receptor, KIR2DL1, Biochem. Biophys. Res. Commun., 387, 3, 575-580 , 2009.09.
Presentations
1. Sasaki-Tabata K, Matsuo A, Fuchida H, Ojida A, Tanaka H and Morimoto S, Cloning, expression and characterization of polyketide synthase gene from Scopolia Japonica, The 9th CSP-KSP-JSP Joint Symposium on Pharmacognosy, 2016.05, BACKGROUND AND PURPOSE: Various important pharmacological compounds such as atropine and scopolamine are included in Scopolia japonica. These compounds are synthesized using ornithine as a starting material, however, it’s unclear which enzyme catalyzes the tropinone synthesis reaction. Hence, in the present study we aimed to identify function and structure of novel polyketide synthase (PKS) from S. japonica.
EXPERIMENTAL APPROACH: A total RNA was extracted from leaves of S. japonica and cDNA was synthesized using a reverse transcriptase. Then, degenerate PCR, 5' and 3' RACE was performed using cDNA as a template. Two novel genes were obtained and expressed in E. coli. The recombinant protein was purified with affinity column and ion exchange column. We analyzed the enzyme activity using HPLC. Moreover, we crystallized and performed crystallographic analysis of PKS.
KEY RESULTS: Two novel genes of PKS from S. japonica were obtained. The recombinant PKSs expressed in E. coli as a soluble fraction was highly purified using affinity and ion exchange column and the enzyme assay with HPLC showed that new compounds were synthesized using some substrates. Moreover, crystallization screening resulted in obtaining needle or plate crystals. We could get single crystals in an optimized condition. The crystal diffracted to 2.0 angstrom at KEK-PF and the structures of PKS were determined using molecular replacement method.
CONCLUSION AND IMPLICATIONS: These PKS genes seem to belong to PKS family because they showed high similarity to other CHS from various plants. These novel findings may contribute to clarifying the mechanism of tropane synthesis..
Membership in Academic Society
  • The Pharmaceutical Society of Japan
  • The Japanese Society of Pharmacognosy
  • The Molecular Biology Society of Japan