||Takeki Hamasaki, Harada Gakuro, Noboru Nakamichi, Shigeru Kabayama, Kiichiro Teruya, Fugetsu, Bunshi, Gong, Wei, Sakata, Ichiro, Sanetaka Shirahata, Electrochemically reduced water exerts superior reactive oxygen species scavenging activity in HT1080 cells than the equivalent level of hydrogen-dissolved water
, PLoS ONE, 10.1371/journal.pone.0171192, 12, 2, 2017.02, Electrochemically reduced water (ERW) is produced near a cathode during electrolysis and exhibits an alkaline pH, contains richly dissolved hydrogen, and contains a small amount of platinum nanoparticles. ERW has reactive oxygen species (ROS)-scavenging activity and recent studies demonstrated that hydrogen-dissolved water exhibits ROS-scavenging activity. Thus, the antioxidative capacity of ERW is postulated to be dependent on the presence of hydrogen levels; however, there is no report verifying the role of dissolved hydrogen in ERW. In this report, we clarify whether the responsive factor for antioxidative activity in ERW is dissolved hydrogen. The intracellular ROS scavenging activity of ERW and hydrogen-dissolved water was tested by both fluorescent stain method and immuno spin trapping assay. We confirm that ERW possessed electrolysis intensity-dependent intracellular ROS-scavenging activity, and ERW exerts significantly superior ROS-scavenging activity in HT1080 cells than the equivalent level of hydrogen-dissolved water. ERW retained its ROS-scavenging activity after removal of dissolved hydrogen, but lost its activity when autoclaved. An oxygen radical absorbance capacity assay, the 2,2-diphenyl-1-picrylhydrazyl assay and chemiluminescence assay could not detect radical-scavenging activity in both ERW and hydrogen-dissolved water. These results indicate that ERW contains electrolysis-dependent hydrogen and an additional antioxidative factor predicted to be platinum nanoparticles..
||Takeki Hamasaki, Noboru Nakamichi, Kiichiro Teruya, Sanetaka Shirahata, Removal Efficiency of Radioactive Cesium and Iodine Ions by a Flow-Type Apparatus Designed for Electrochemically Reduced Water Production., PLoS ONE, 10.1371/journal.pone.0102218, 9, 7, 2014.06, The Fukushima Daiichi Nuclear Power Plant accident on March 11, 2011 attracted people's attention, with anxiety over possible radiation hazards. Immediate and long-term concerns are around protection from external and internal exposure by the liberated radionuclides. In particular, residents living in the affected regions are most concerned about ingesting contaminated foodstuffs, including drinking water. Efficient removal of radionuclides from rainwater and drinking water has been reported using several pot-type filtration devices. A currently used flow-type test apparatus is expected to simultaneously provide radionuclide elimination prior to ingestion and protection from internal exposure by accidental ingestion of radionuclides through the use of a micro-carbon carboxymethyl cartridge unit and an electrochemically reduced water production unit, respectively. However, the removability of radionuclides from contaminated tap water has not been tested to date. Thus, the current research was undertaken to assess the capability of the apparatus to remove radionuclides from artificially contaminated tap water. The results presented here demonstrate that the apparatus can reduce radioactivity levels to below the detection limit in applied tap water containing either 300 Bq/kg of 137Cs or 150 Bq/kg of 125I. The apparatus had a removal efficiency of over 90% for all concentration ranges of radio-cesium and -iodine tested. The results showing efficient radionuclide removability, together with previous studies on molecular hydrogen and platinum nanoparticles as reactive oxygen species scavengers, strongly suggest that the test apparatus has the potential to offer maximum safety against radionuclide-contaminated foodstuffs, including drinking water. Removal Efficiency of Radioactive Cesium and Iodine Ions by a Flow-Type Apparatus Designed for Electrochemically Reduced Water Production. Available from: https://www.researchgate.net/publication/264010807_Removal_Efficiency_of_Radioactive_Cesium_and_Iodine_Ions_by_a_Flow-Type_Apparatus_Designed_for_Electrochemically_Reduced_Water_Production [accessed May 2, 2017]..
||Hamasaki T, Aramaki S, Imada T, Teruya K, Kabayama S, Katakura Y, Otubo K, Morisawa S, Shirahata S. , Induction of caspase-3-dependent apoptosis by electrolyzed reduced water/platinum nanoparticles in cancer cells., Cell Technology for Cell Products 87-89. (2007)., 2007.07.
||Hamasaki T, Kashiwagi T, Aramaki S, Imada T, Komatsu T, Li Y, Teruya K, Katakura Y, Kabayama S, Otsubo K, Morisawa S, Shirahata S., Suppression of cell growth by platinum nanocolloids as scavengers against reactive oxygen species. , Animal Cell Technology Meets Genomics 249-251. (2005), 249-251, 2005.07.