Kyushu University Academic Staff Educational and Research Activities Database
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Keishi Sakaguchi Last modified date:2019.05.27

Associate Professor / Fisheries Research Institute of Karatsu, Department of Joint Research
Faculty of Agriculture

Academic Degree
Field of Specialization
Molecular Biology, Biochemistry, bioengineering
Outline Activities
* Development molecular techniques applicable to aquaculture industry
Research Interests
  • Study on Structure and Function of Glycosphingolipid-specific Glycohydrolase and Glycosyltransferase
    keyword : glycosphinglipid, sphingolipid-degrading enzyme, catalytic mechanism, glycosyltransferase, in vivo function, zebrafish, knockdown
  • Study on structure-function relationship of seaweed polysaccharide degrading enzyme(s)
    keyword : seaweed polysacchride degrading enzyme, catalytic mechanism, X-ray crystal structure analysis
  • Study on a HUFA-synthesizing machinery of marine thraustochytrids
    keyword : thraustochytrids, transformation system, genetic modification, highly-unsaturated fatty acids, DHA, EPA, genome analysis, biofuel
  • Studies on the development of the efficient aquaculture methods
    keyword : genetically modified animal, genome editing, animal factory, model animal, aquaculture
Academic Activities
1. Sakaguchi K, Kiyohara M, Watanabe N, Yamaguchi K, Ito M, Kawamura T, Tanaka I., Preparation and preliminary X-ray analysis of the catalytic module of beta-1,3-xylanase from the marine bacterium Vibrio sp. AX-4., Acta Crystallogr D Biol Crystallogr., 10.1107/S0907444904013411, 60, Pt 8, 1470-1472, 2004.08.
2. Kiyohara M, Sakaguchi K, Yamaguchi K, Araki T, Nakamura T, Ito M., Molecular cloning and characterization of a novel beta-1,3-xylanase possessing two putative carbohydrate-binding modules from a marine bacterium Vibrio sp. strain AX-4., Biochem J., 10.1042/BJ20050190, 388, Pt 3, 949-957, 2005.06.
3. Kiyohara M, Sakaguchi K, Yamaguchi K, Araki T, Ito M., Characterization and application of carbohydrate-binding modules of beta-1,3-xylanase XYL4., J Biochem., 146, 5, 633-641, 2009.11.
4. Kobayashi T, Sakaguchi K, Matsuda T, Abe E, Hama Y, Hayashi M, Honda D, Okita Y, Sugimoto S, Okino N, Ito M., Increase of eicosapentaenoic acid in thraustochytrids through thraustochytrid ubiquitin promoter-driven expression of a fatty acid {delta}5 desaturase gene., Appl Environ Microbiol., 77, 11, 3870-3876, 2011.01.
5. Goddard-Borger ED, Sakaguchi K, Reitinger S, Watanabe N, Ito M, Withers SG., Mechanistic insights into the 1,3-xylanases: useful enzymes for manipulation of algal biomass., J Am Chem Soc., 134, 8, 3895-3902, 2012.02.
6. Sakaguchi K, Matsuda T, Kobayashi T, Ohara J, Hamaguchi R, Abe E, Nagano N, Hayashi M, Ueda M, Honda D, Okita Y, Taoka Y, Sugimoto S, Okino N, Ito M., Versatile transformation system that is applicable to both multiple transgene expression and gene targeting for Thraustochytrids., Appl Environ Microbiol., 78, 9, 3193-3202, 2012.05.
7. Matsuda T, Sakaguchi K, Hamaguchi R, Kobayashi T, Abe E, Hama Y, Hayashi M, Honda D, Okita Y, Sugimoto S, Okino N, Ito M., Analysis of Δ12-fatty acid desaturase function revealed that two distinct pathways are active for the synthesis of PUFAs in T. aureum ATCC 34304., J Lipid Res., 53, 6, 1210-1222, 2012.06.
8. Junichiro Ohara, Keishi Sakaguchi, Yuji Okita, NOZOMU OKINO, Makoto Ito, Two fatty acid elongases possessing C18-D6/C18-D9/C20-D5 or C16-D9 elongase activity in Thraustochytrium sp. ATCC 26185., Marine Biotechnology, 15, 4, 476-486, 2013.08.
Membership in Academic Society
  • The Japanese Society of Fisheries Science
  • Japan Society for Bioscience, Biotechnology, and Agrochemistry
  • Japanese Society for Marine Biotechnology