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Yu Hoshino Last modified date:2017.05.26

Associate Professor / Molecular System and Biosystem Engineering,
Department of Chemical Engineering
Faculty of Engineering

Graduate School
Undergraduate School

Academic Degree
Ph. D
Field of Specialization
Biomolecular Engineering, Protein Science, Polymer Science, Molecular Recognition, Colloid & Surface Chemistry
Outline Activities
We have been developing general procedures to create synthetic nano materials, that function as antibody and enzymes, from inexpensive and stable acrylic monomers.

1, Development of Plastic Antibodies
General procedures for the creation of synthetic nanoparticles with biomacromolecular recognition sites are of significant interest as a route to stable, robust, and mass-produced substitutes for antibodies. Ideally, recognition of complex biological targets, including proteins, peptides, and carbohydrates, requires multiple functional groups that contact target molecules by a combination of electrostatic, hydrogen-bonding, van der Waals, and/or hydrophobic interactions. We have shown that copolymerization of optimized combination and ratio of functional acrylic-monomers creates synthetic polymer materials with molecular recognition sites for peptides and proteins. In addition to optimizing functional complementarity, the affinity sites can be further enhanced by molecular imprinting and/or affinity purification process. Those particles are capable of recognizing, neutralizing, and clearing toxic peptide and proteins even in the bloodstream of living animals. However, in contrast to antibodies whose exact sequence can be determined and cloned, polymerized materials result in heterogeneous structures with a distribution of recognition sites. Our goal is to achieve general strategy to create plastic antibodies with uniform molecular structure as "monoclonal plastic antibodies".

2, Development of Plastic Enzymes
Life is a dissipate structure appears in the non-equilibrium open system. To maintain the structure, enzymes mediates most reactions in the body including molecular transportation, energy conversion and molecular conversion that are necessarily for the life. To keep the life alive, it is important for the each enzyme to recognize and bind to specific molecules and ions and catalyze specific reaction just in time. It is also very important to control binding and dissociating kinetics to keep the dissipate structure.
One of the challenges for us is to construct synthetic molecular system like living life. So far, we found that target binding and dissociation kinetics of nanoparticles can be tuned by conformation change and polymer density of the particles. Binding constant to target proteins and ions can be reversibly switched on/off by temperature-induced phase transition of the polymer chains. By applying the fundamental findings, we are trying to develop inexpensive and scalable chemical process for the conversion of low temperature waste heat in to reusable chemical potential.

(1) PNAS 109 (1), 33-38, 2012
(2) JACS 134, 15765-15772, 2012
(3) JACS 130, 15242-15243, 2008
(4) JACS 132, 13648-13650, 2010
(5) JACS 136, 1194-1197, 2014
(6) JACS 132, 6644-6645, 2010
(7) JACS 137, 10878-10881, 2015
(8) Angew. Chem. Intl Ed. 51, 2405-2408, 2012
(9) JACS 134, 15209-15212, 2012
(10) Biomacromolecules 15, 541–547, 2014
(11) JACS 138, 4282-4285, 2016
(12) Biomacromolecules,16, 411–421, 2015
(13) Advanced Materials 26, 2449–2606, 2014
(14) JACS 134, 18177-18180, 2012
(15) Chem. Sci. 6, 6112, 2015
(16) Angew. Chem. Intl Ed. 53, 2654–2657, 2014
Research Interests
  • Development of CO2 Separation Membranes
    keyword : CO2
  • Development of protein separation media
    keyword : Synthetic Polymer, Moleculr Recognition, Antibody
  • Development of Materials for Heat Energy Transduction
    keyword : Heat Energy Transduction
  • Development of Energy Efficient CO2 Adsorbent
    keyword : CO2
  • Development of Plastic Antibodies
    keyword : Synthetic Polymer, Moleculr Recognition, Antibody
Academic Activities
1. Masahiko Nakamoto, Tadashi Nonaka, Yoshiko Miura, Kenneth J. Shea, Yu Hoshino*, Design of Synthetic Polymer Nanoparticles that Facilitate Resolubilization and Refolding of Aggregated Positively Charged Lysozyme, J. Am. Chem. Soc., 138, 2016.02, [URL].
2. Lee Haejoo, Yu Hoshino*, Yusuke Wada, Yuka Arata, Atsushi Maruyama, Yoshiko Miura, Minimization of synthetic polymer ligands for specific recognition and neutralization of a toxic peptide, J. Am. Chem. Soc., 137, ASAP, 2015.09, [URL].
3. Mengchen Yue, Yu Hoshino*, Yoshiko Miura, Design Rationale of Thermally Responsive Microgel Particle Films That Reversibly Absorb Large Amounts of CO2: Fine Tuning the pKa of Ammonium Ions in the Particles, Chem. Sci., ASAP, 2015.08, [URL].
4. Keiichi Yoshimatsu, Hiroyuki Koide, Yu Hoshino, Kenneth J. Shea, Preparation of abiotic polymer nanoparticles for sequestration and neutralization of a target peptide toxin, Nature protocols, 10, 595–604, 2015.04, [URL].
5. Yoke-Ming Wong, Yu Hoshino*, Kumar Sudesh, Yoshiko Miura, Keiji Numata*, Optimization of poly (N-isopropylacrylamide) as an artificial amidase, Biomacromolecules, 16, 411–421, 2015.01, [URL].
6. Yusuke Wada, Haejoo Lee, Yu Hoshino*, Shunsuke Kotani, Kenneth J. Shea, Yoshiko Miura, Design of multi-functional linear polymers that capture and neutralize a toxic peptide: a comparison with cross-linked nanoparticles, Journal of Materials Chemistry B, 3, 1706-1711, 2015.01, [URL].
7. Yu Hoshino*, Yuka Arata, Haejoo Lee, Yusuke Yonamine, Shih-Hui Lee, Aki Yamasaki, Ryousuke Tsuhara, Katsuhiko Yano, Kenneth J Shea, Yoshiko Miura, Preparation of nanogel-immobilized porous gel beads for affinity separation of proteins: fusion of nano and micro gel materials, Polymer Journal , 47, 220–225, 2015.01, [URL].
8. Adam Weisman, Yingyao Allie Chen, Yu Hoshino, Huiting Zhang, Kenneth J. Shea, Engineering Nanoparticle Antitoxins Utilizing Aromatic Interactions, Biomacromolecules, 15, 3290–3295, 2014.06, [URL].
9. Yu Hoshino*, Ryohei C. Ohashi, Yoshiko Miura, Rational Design of Synthetic Nanoparticles with a Large Reversible Shift of Acid Dissociation Constants: Proton Imprinting in Stimuli Responsive Nanogel Particles, Advanced Mater, 26, 2449–2606, 2014.03, [URL].
10. Mengchen Yue, Yu Hoshino*, Yukinori Ohshiro, Kazushi Imamura, Yoshiko Miura, Temperature-Responsive Microgel Films as Reversible Carbon Dioxide Absorbents in Wet Environment, Angew. Chem. Int. Ed., 126, 2692–2695, 2014.01, [URL].
11. K. Yoshimatsu, T. Yamazaki, Yu Hoshino, L. F. Epstein, L. P. Miranda, P. Tagari, J. M. Beierle, Y. Ynamine, K. J. Shea, Epitope Discovery for a Synthetic Polymer Nanoparticle: A New Strategy for Developing a Peptide Tag, J. Am. Chem. Soc., 136, 1194–1197, 2014.01, [URL].
12. Masahiko Nakamoto, Yu Hoshino*, Yoshiko Miura*, Effect of Physical Properties of Nanogel Particles on the Kinetic Constants of Multipoint Protein Recognition Process, Biomacromolecules, 15, 541–547, 2013.12, [URL].
13. Yonamine, Yusuke, Yoshimatsu, Keiichi, Lee, Shih-Hui, Yu Hoshino, Okahata, Yoshio, Shea, Kenneth J, Polymer Nanoparticle-Protein Interface. Evaluation of the Contribution of Positively Charged Functional Groups to Protein Affinity, ACS APPLIED MATERIALS & INTERFACES, 5, 2, 374-379, 2013.01.
14. Y. Hoshino*, K. Imamura , M. Yue , G. Inoue , Y. Miura*, Reversible absorption of CO2 triggered by phase transition of amine-containing micro- and nano-gel particles, J. Am. Chem. Soc., 134, 18177-18180, 2012.10, [URL].
15. S.-H. Lee, Y. Hoshino, A. Randall, Z. Zeng, P. Baldi, R.-a. Doong and K. J. Shea, Engineered Synthetic Polymer Nanoparticles as IgG Affinity Ligands, J. Am. Chem. Soc., 134, 15765−15772, 2012.09, [URL].
16. Y. Hoshino*, M. Nakamoto, and Y. Miura*, Control of protein-binding kinetics on synthetic polymer nanoparticles by tuning flexibility and inducing conformation changes of polymer chains, J. Am. Chem. Soc., 134, 15209−15212, 2012.09, [URL].
17. Y. Yonamine, Y. Hoshino, and K. J. Shea, An ELISA-mimic screen for synthetic polymer nanoparticles with high affinity to target proteins, Biomacromolecules, 13, 2952–2957, 2012.07, [URL].
18. K. Yoshimatsu, B. K. Lesel, Y. Yonamine, J. M. Beierle, Y. Hoshino, and K. J. Shea, Temperature-Responsive “Catch and Release” of Proteins by using Multifunctional Polymer-Based Nanoparticles, Angew. Chem. Int. Ed., 51, 2405–2408, 2012.03, [URL].
19. Y. Hoshino*, H. Koide, K. Furuya, W. W. Haberaecker III, S. Lee, T. Kodama, H. Kanazawa, N. Oku, and K. J. Shea*, The Rational Design of a Synthetic Polymer Nanoparticles that Neutralizes a Toxic Peptide in Vivo, Proc. Natl. Acad. Sci. USA, 109, 33-38, 2012.01, [URL].
20. Y. Hoshino, and K. J. Shea*, Evolution of Plastic Antibodies, J. Mat. Chem., 2010.10, [URL].
21. Y. Hoshino*, W. W. Haberaecker III, T. Kodama, Z. Zeng, Y. Okahata, and K. J. Shea*, Affinity Purification of Multifunctional Polymer Nanoparticles, J. Am. Chem. Soc., 132, 13648-13650, 2010.09, [URL].
22. Y. Hoshino*, H. Koide, T. Urakami, H. Kanazawa, T. Kodama, N. Oku, and K. J. Shea*, Recognition, Neutralization, and Clearance of Target Peptides in the Bloodstream of Living Mice by Molecularly Imprinted Polymer Nanoparticles: A Plastic Antibody, J. Am. Chem. Soc., 132, 6644–6645, 2010.04, [URL].
23. Z. Zeng, Y. Hoshino, A. Rodoriguez, H. Yoo, and K. J. Shea, Synthetic Polymer Nanoparticles with Antibody-Like Affinity for a Hydrophilic Peptide, ACS nano, 4, 199–204, 2009.12, [URL].
24. Y. Hoshino*, T. Urakami, T. Kodama, H. Koide, N. Oku, Y. Okahata, and K. J. Shea*, Design of Synthetic Polymer Nanoparticles that Capture and Neutralize Toxic Peptide, Small, 5, 1562–1568, 2009.03.
25. Y. Hoshino, T. Kodama, Y. Okahata, and K. J. Shea, Peptide Imprinted Polymer nanoparticles “Plastic Antibodies”, J. Am. Chem. Soc., 130, 15242-15243, 2008.10, [URL].
26. Y. Hoshino, T. Kawasaki, and Y. Okahata, Effect of Ultrasound on DNA Polymerase Reactions: Monitoring on a 27-MHz Quartz Crystal Microbalance, Biomacromolecules, 7, 682–685, 2006.02, [URL].
27. Y. Hoshino, S. Tajima, H. Nakayama, and Y. Okahata, A RNA-aligned Film Prepared from a RNA-Lipid Complex, Macromol. Rapid Commun., 23, 253-255, 2002.03, [URL].
1. 星野 友, 大橋 良平, 三浦 佳子, Development of Enzyme-Mimic Proton Transfer Systems, IUMRS-International Conference on Electronic Materials (IUMRS-ICEM 2014), 2014.08.27.
2. 星野 友, 大橋 良平, 三浦 佳子, Preparation of Temperature Responsive Nanogels with Carboxylic Acids which Undergo Large and Reversible pKa Shift, IUMRS-International Conference on Electronic Materials (IUMRS-ICEM 2014), 2014.08.27.
3. 星野 友, 大橋良平, 三浦 佳子, Preparation of Proton Imprinted Nanoparticles with Switchable pKa Values: Toward Plastic Enzymes, MRS Fall Meeting,2013, 2013.12.02.
4. Yu Hoshino, Kazushi Imamura, Mengchen Yue, Yoshiko Miura, Reversible absorption of CO2 by aqueous solution of amine-containing poly-N-isopropylacrylamide particles, The 244th ACS National Meeting , 2013.04.10.
5. Ryohei Ohashi, Yu Hoshino, Yoshiko Miura, Reversible absorption of protons by temperature-responsive gel-particles, The 244th ACS National Meeting , 2013.04.10.
6. Yu Hoshino, Mengchen Yue, Yoshiko Miura, Hydrogel films consisting of temperature-responsive nanogels as an absorbent to capture CO2 reversibly, The 244th ACS National Meeting , 2013.04.10.
7. LEE HAEJOO, Yusuke Wada, Yu Hoshino, Yoshiko Miura, Preparation and characterization synthetic polymer ligands that recognize target peptides with multipoint interaction, The 244th ACS National Meeting , 2013.04.08.
8. LEE HAEJOO, Yusuke Wada, Yu Hoshino, Yoshiko Miura, Minimization of Polymer Ligands that Interact with Targeted Peptide, Soft-interfaces Mini-symposium 2013-Physical Chemistry and Characterization of Soft-interfaces-(SIMS2013) , 2013.03.14.
9. Mengchen Yue, Yu Hoshino, Yoshiko Miura, Study on reversible CO2 absorption by thermo-responsive hydrogel films, Soft-interfaces Mini-symposium 2013-Physical Chemistry and Characterization of Soft-interfaces-(SIMS2013) , 2013.03.14.
Membership in Academic Society
  • Materials Research Society
  • American Chemical Society
  • The Chemical Society of Japan
  • The Electrochemical Society of Japan
  • The Society of Chemical Engineers
  • The Society of Polymer Science, Japan
  • for the work of "Preparation of Temperature Responsive Nanogels with Carboxylic Acids which Undergo Large and Reversible pKa Shift"